DE3120258A1 - Device for the withdrawal of blood, and its use for the separation of blood cells - Google Patents

Abstract

A device for the withdrawal of blood by puncturing of blood vessels and for the isolation of cells from blood is described, which device consists of two communicating elements with two or three chambers.

Description

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BEHRINGWERKE AKTIENGESELLSCHAFT Hoe 81 / B 00 9 - Ma <+06 ANR: 1 0 00 764 Dr Ha / Bl.

Device for drawing blood and its use for separating blood cells

The invention relates to a device for removal of blood with a device for puncturing blood vessels, combined with a reaction vessel for the Isolation of cells from blood consisting of two communicating elements with three or where appropriate two chambers.

The elements for the pretreatment of the blood and the extraction of the blood are located in each of the chambers of the elements Lymphocytes required fluids. By moving or twisting the two elements against each other Openings released so that the contents of the elements can mix.

From DE-AS 20 12 607 a two-chamber syringe is known, which consists of a cylinder with a sliding one Piston and a separator. The cylinder wall has a narrowed cross section. By moving of the piston, the separating element performs a tilting movement. The contents of the two chambers can then mix .

A syringe that can be filled, the two telescopic to each other Has displaceable coaxial chambers is described in DE-OS 19 65 990.

This prior art cannot be used to isolate blood cells of a type from whole blood in one work step.
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The invention is therefore based on the object of designing a device of the specified type so that blood taken by puncturing blood vessels can be further processed in such a way that refills are not necessary, the sterile condition is guaranteed and blood cells can be won.

This object is achieved according to the invention with a device solved, which has two separable chambers, one of which allows an increase in volume. The device consists of two telescoping one inside the other sliding tubes, the outer tube being one-sided is closed and possibly an openwork Has intermediate bottom, the inner tube is provided with a bottom, which is optionally perforated, in which If the geometrical shapes of the two openings are matched to one another in such a way that they match the unopened part the openwork part can cover, or where that can be covered with a bottom. closed inner tube has lateral openings near the bottom on its circumference, the inner tube has a displaceable therein Piston possesses, with a continuously hollow piston rod, the bore of which on a sealing elevation of a possibly existing intermediate floor meets and one Has a cone for attaching a cannula.

An essential feature of the device according to the invention is that by rotating the inner tube, the openings at the bottom of the opening-free places of the intermediate floor of the outer tube or through, possibly rotating, moving the inner tube side openings are closed, creating two separate chambers are formed. By moving the piston inside the inner tube,

on the other hand one by the possibly existing The intermediate bottom of the inner tube and the piston plate limited third chamber formed.

It is also possible to use the piston bore and the inner tube to close.

The invention relates to a device consisting from a suitable for the absorption of liquids Tube closed on one side with a second tube which can be moved telescopically inside and which has a cover is completed, a piston displaceable therein with a continuously hollow piston rod and at the other end a closure that can be turned, shifted -bench or rotating displacement of the inner tube opens a passage to the otherwise closed tube.

Thus, the device can be maintained fill, ship and then under sterile conditions the blood in the laboratory without the addition of further reaction liquids to edit.

Two preferred embodiments are shown as drawings and are described in more detail below. 25th

It shows:

Figure 1 - a sectional view of an embodiment of the device according to the invention In addition:

Figure 2 - a longitudinal section of the head with Closing cap for the inner tube and the piston bore

Figure 3 - a top view of the piston with cone for

the cannula and guide of the plunger

Figure h - A top view of a recess of the intermediate floor of the inner tube

Figure 5 - A section along the plane V-V in

Figure, 4
5

Figure 6 - the bottom of the inner tube in an enlarged

scale

FIG. 7 - a view from below of the floor according to FIG. 6 10

FIG. 8 - a section VIII - VIII through the floor according to FIG. 7.

As is shown by way of example in FIG. 1, the device has two elements C 1 + 2) with 3 chambers (3, 4 + 5). The outer element CO is a cylindrical tube open on one side with a recess as an intermediate floor (6). This retraction 6 has two openings (7 + 8), which are advantageously opposite each other like an angular circle are attached, as in the bottom 10.

In element 1, a cylindrical one runs telescopically Tube (2). It can be connected by a bayonet catch (9) be locked with element 1.

The bottom (10) is, for example by means of a thread, connected to element 2. It has cruciform sealing lips (11) and a sealing ring (12) on the underside. Opposite, between sealing cross (11) and sealing ring (12), the bottom (10) has two quarter circles Openings (13, 14) which are closed in the assembled state of the elements 1 and 2 by the remaining ones Halves of the collection (6) and the bottom (10) are sealed. The recovery (15) of the second element has the Function to seal the bore of the piston and the piston rod (16). The retraction (15) is designed as a web. the Chamber (4) is closed by the two sealing rings (17) of the

Piston sealed. Immediately above the piston there is a predetermined breaking point (18) for breaking off the Piston rod.

The opening of element 2 has an external thread O9) provided for receiving a closure cap (20). These Closure cap (20) has a conical elevation (21) on the inside to seal the piston bore.

The cannula for puncturing the blood vessel is placed on the cone (22) of the piston rod (16).

The inside of the element 2 has two rings (23 + 24) on the upper edge. The function of the ring (23) to lock the piston. Ring (24) is intended to prevent the piston from breaking out of the element if the piston rod breaks off 2 is pushed out. The ring (24) also has a guide function for the piston rod resulting from this Basic three-legged (Fig. 3 0 is designed.

Another embodiment is shown in FIGS shown.

It shows :

Figure .9 - a sectional view of this embodiment of the device according to the invention
In addition:

Figure 10 - a partial. Section of the sealing plug

Figure 11 - a side view of the sealing plug

Plugging
35

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As shown in Figure 9, this embodiment of the device has two telescopically nested ones Tubes (25 and 26,27). The outer element (25) is preferably a cylindrical one that is open on one side Tube with a cone (29) which is matched to the bottom part (27) of the inner tube (26).

The inner element, consisting of the individual parts (26,27), is closed on both sides, whereby the bottom part (27) is laterally provided with radial openings (28).

In the assembled state, the two-sided Cone of inner (26,27) or outer element (25) that the openings (28) in the bottom part (27) of the inner element (26,27) are sealed and the chamber (30) both in the direction of the chamber (31) and in the direction Outside atmosphere is complete. For better dosage the opposing axial displacements of the two elements 25 and 26,27 are internal (26,27) as well Outer element (25) is provided, for example, with interlocking threads (32). The desired axial Movement is made by turning the two in opposite directions Elements (25 and 26,27) brought about. Turn in one Direction causes the cone sealing surfaces to be pressed together more strongly, turning in the other direction are the openings (28) in the bottom of the inner element free and sorbitol connects the chamber (30) with the chamber (31).

Inside the inner tube (26) there is a piston (33) which separates the chamber (30) from the chamber (34) and is detachably connected to the piston rod (35).

The piston rod (35) is in the opening of the cover guided by the inner tube (26). The mouth is in shape a circumferential collar (36) with inner and outer cones and a circumferential outer locking groove.

The hollow piston rod C 35) has at the free end a rotatable handle (37) which is locked in the annular groove (38) and a cone (39) for attaching the cannula, as well as an enlarged cross-section in the form of an outer cone, which in the normal position of the piston sealingly closes the inner cone of the collar (36) from the inner tube (26) and at the same time limits the stroke of the piston (33) in the direction of the bottom part of the inner element (27), so that the chamber (3 ¹ O, as required for sterility reasons, against the outside atmosphere * is sealed.

The hollow piston rod (35) is provided with a longitudinal groove (40) which ensures that the air present in chamber (34), which is displaced by piston G3) when blood is drawn, through the opening between the longitudinal groove (40) and the opening of the lid of the inner tube (26) is present, can escape.

The hollow piston rod (35) is, for example, by a thread (41) ' connected to the piston (33), the piston through a recess (42), which with the cam (43) on the inside of the lid of the inner tube (-26) after Blood withdrawal is engaged and is thus prevented from turning when the piston rod is unscrewed.

The sealing plug (44) seals by means of an inner plug (45) the bore of the hollow piston rod (35) and the cone (39) and holds the piston rod (35) sealingly in the starting position, which is achieved by the two locking tubes (46), which engage in the outer locking groove is achieved.

During the stopper (44) its task until the blood sample fulfilled, sealing plug (47) according to Figurio and 11 needed after blood draw. 35

Is unscrewed from the KolbenC33) the hollow piston rod (35), the closure plug C ^ is screwed 7) in the plunger until such intervene in plugs of ¹ O, the latching ears C ^ * D in the Außenrastnut and simultaneously the inner cone of the circumferential Collar C36) of the inner tube C26) is sealed.

Both plugs (Λ70 and 0 * 5) are used for Loosen the plug of the rate tubes, ("46) or (48>) canceled. .

It is at the discretion of the person skilled in the art, the inventive To further develop the principle. So are the ones given Embodiments can be varied in many ways, in particular the design and location of the intermediate floor, the closures and the closures, the piston rod, the Cannula opening and the main elements in general.

As a material, substances are used that are customary for the production of test tubes, blood sampling devices or injection syringes.

Clear polystyrene, polyethylene, and polypropylene are preferred.

In the following, the handling of the in FIGS to 8 embodiment shown by way of example be written:

One of them is used to obtain the lymphocytes Chamber C5) an aqueous solution with a precisely set density of 1.077 g / l. She sits down as follows together :

.

CR) *

23.3 ml Uromlro ^ ^ 380 80% ί g CR-X-ray contrast medium)

+ 6.0 g FIcoll CLymphocyte separation med J from Flow)

+ 130.0 ml H ₂ O dist.

The second chamber C ¹ O contains a dilution solution to the blood and an anticoagulant. The dilution solution is made up as follows:

7.5 G in NaCl ad 100 0 ml H, _? O least. 0.75 G KCl 0.1 G Na ₂ H PO ₄ 2 H ₂ O 0.12 G substance d i ent for example 0.5 G As ger i nn-obstructing Hepar

After puncturing the blood vessel, hold the cannula and plunger rod with two fingers and pull back the blood collection device. The recovery C15) opens the bore of the piston rod CI6) and the blood flows into the filled with diluent and anticoagulant substance chamber C3 + ¹ O. The device is retracted so far., That the groove C49) of the piston in the sealing ring C23) engages . The amount of blood corresponding to the volume of the dilution liquid has now been drawn in.

The piston rod O6) is now at the predetermined breaking point C18) canceled and can be discarded. The blood collection device can be closed with the cap C20) and is thus dispatchable.

To separate the lymphocytes from the thinned blood the device is handled as follows:

The cap C20) is unscrewed. By twisting the Elements 1 and 2 against each other is the bayonet lock

opened. In this state, the two circle openings open from the ground (10) and retraction (6). Element 2 is now carefully pulled out of element 1.

The sugar solution is now covered with the thinned blood. Element 1 is now long at 200 g for 20 minutes centr i fug i ed.

When the centrifugation is complete, the drawer is located above the drawer (6) a layer with lymphocytes. The confiscation

(6) has the advantage that a pipette can be placed on it can and receives all lymphocytes when sucking up.

An exemplary description of the handling follows the embodiment shown in Figures g to 11:

A solution with a density of 1.077 g / l is located in one chamber (3i) to collect lymphocytes. It is composed as stated above.

The second chamber £ 30) contains a diluting solution for the blood and an anticoagulant substance. The dilution solution is also composed as indicated above.

An example is used as an anticoagulant substance Hepar in.

Before taking blood, remove the plug η (Α4), places a cannula on the cone (39) of the hollow piston rod (35), punctures the blood vessel and pulls the blood collection device while holding the piston rod by the handle (37). The resulting negative pressure ensures that the blood through the cannula and the hollow piston rod (35) flows into the chamber (30) that is already containing thinning inhibitor Substance is filled. The chamber (30) is enlarged

by the piston movement at the expense of the chamber the air from chamber ^ **) through the piston C33) over the existing opening between the longitudinal groove 00) of the piston rod C35) and the guide hole in the lid of the inner tube C26) escapes.

If the piston C33) reaches the cover of the inner element, once the blood collection has been completed, the hollow piston rod C35) is unscrewed from the piston C33) and through Screw a plug C ^ 7) into the piston C33) replaced. The device is now ready for dispatch.

To separate the lymphocytes from the thinned blood the device is handled as follows:

The plug C47) - is removed for ventilation. By counter-rotating the elements 25 and 26,27 are the tubes pulled apart until the openings C ^ S) in the bottom of the inner element C ^ 6.27) connect chamber 30 and chamber C31) and blood and dilution solution slowly and radially from the inner element (26,27) to the Inner walls of the outer element (25) flow into chamber (3i) and overlay the sugar solution already there.

The inner element (C26,27) is now removed while the Außene1ementC25) if necessary with a not shown Closure cap is provided and at 200 g Centrifuged for 20 min. After the There is centrifugation in the area of the cone Layer of lymphocytes. This retraction through the cone has the advantage that the placement of a pipette for Aspiration of lymphocytes is facilitated.

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Claims (2)

Patent claims: A device characterized by a for Absorption of liquids suitable on one side closed tube with a telescopic in it movable second tube, which is closed with a cover, and by a movable one therein Piston with a continuously hollow piston rod and an end at the other end that goes through Rotating, moving or rotating moving the inner tube creates a passage to the one-sided closed Tube opens. 2. Use of a device according to claim 1 for the separation of blood cells.