DE19647811A1 - Hydrolysis of nucleic acids in foodstuffs - Google Patents

Abstract

Process for hydrolysing nucleic acids in foodstuffs comprises adding nucleases to the foodstuffs.

Description

The invention relates to a method / treatment for the degradation of nucleic acids in food of vegetable and animal origin.

It is known that the population against the backdrop of a growing market genetically modified food is uncertain about the extent to which it poses a risk there is a food intake of these foods. It is therefore difficult for industry to do that Products known to be, or possibly only, genetically engineered include process to sell on the market.

On the other hand, there is a demonstrable additional allergy risk for the population the consumption of these products.

The additional allergy risk is based on the fact that genetic engineering changes an existing organism by inserting foreign genetic information for the concerned DNA and proteins that do not occur naturally in foods can be detected.

Foreign biomolecules in foods modified in this way can thus be consumed when consumed trigger allergic reaction, even if there has been no reaction to the natural product had occurred.

There is therefore a need for a product that already contains genetic material Food consumption decomposes. According to the invention, the solution to this problem is that endo- and Exonucleases are able to hydrolyze nucleic acids (DNA and RNA), i.e. H. in Disassemble oligonucleotides and nucleotides.

The invention thus relates to the method characterized in the claims.

The process according to the invention is subject to all those which can be eaten through food Nucleic acids (plasmids, DNA, RNA, chromosomal DNA).

DNSases (deoxyribonucleases) are already being used in therapy of people suffering from cystic fibrosis, in which highly concentrated solutions of the nuclease directly Bronchi is supplied to dissolve the mucus made of DNA there.

The effect of DNAse is based on the catalytic cleavage of the phosphodiester bond in Nucleic acids and the associated dissolution of the viscous bronchial mucus.

The additional uptake of nucleases (deoxyribonucleases, Ribonucleases) with food decomposes genetic material in an analogous manner.

The nucleases are soon broken down by the body and damage the human organism demonstrably not.

The taking of nucleases according to the invention thus contributes to complete pre-digestion intact genetic material. This is the subject of the general discussion. There there is uncertainty among consumers with regard to genetic engineering genetically manipulated foods and products from their further processing literally boycotted.

The worldwide trade network is so complexly structured and networked that the actual It is difficult for individual consumers to swell individual components of a food are understandable.

So there is also uncertainty on the part of the population if the provider of the Guarantees the naturalness of its products. A labeling requirement under discussion will cannot clarify any component of the food in this regard.  

The use of nucleases according to the invention allows the buyer to freely Decision also to consume products that may have been genetically modified Contain raw materials.

Based on the publicly discussed danger of so-called "gene tomatoes" or "Genetic soybeans" may go out, the use of nucleases according to the invention can be certain contribute to the consumption of these and similar foods in terms of ingestion genetically manipulated genetic material can be done without hesitation.

Ribonucleases and are suitable for carrying out the process according to the invention Deoxyribonucleases, primarily from pancreas or other suitable sources.

Suitable processes for obtaining nucleases of appropriate purity already exist.

Solid nuclease forms have proven to be a suitable form of application in the sense of the invention. Preparations or solutions consisting of the respective lyophilized enzyme in the presence a carrier or auxiliary or dissolved in stabilizing buffer solutions. The Intake can thus take place via tablets and granules or drops.

The nuclease concentration of a unit to be administered depends on the activity of the respective enzyme and is between 0.001 and 100 ppm.

The following examples are intended to illustrate the invention:

example 1

2 mg of herring sperm DNA was added to 10 ml of a human saliva sample. The Sample was mixed with 10 µl of a solution of DNAse I with a concentration of 0.01 ppm at 37 ° C incubated. The DNA was completely broken down into its subunits after only 30 seconds. Of the Evidence was provided by conductometry of the increase in the conductance of the solution.

Example 2

The cell nucleus disruption of tomatoes is highly viscous and forms due to the released DNA a tough slime. By adding nuclease solutions in a concentration of 0.1 ppm and incubation at 37 ° C, the solution immediately becomes thin, because the DNA is broken down.

Claims (5)

1. A process for the hydrolysis of nucleic acids in foods, characterized in that in addition nucleases are added to the food. 2. The method according to claim 1, characterized in that ribonucleases as nucleases and deoxyribonucleases from any suitable source. 3. The method according to claim 1 and 2, characterized in that nucleases in the form of Tablets or granules administered 4. The method according to claim 1 and 2, characterized in that nucleases in the form of Solutions administered. 5. The method according to claim 1, characterized in that a nuclease concentration of 0.001-100 ppm per unit to be administered is used.