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DE1642661A1 - Verfahren zur Gewinnung von Urokinase - Google Patents

Verfahren zur Gewinnung von Urokinase

Info

Publication number
DE1642661A1
DE1642661A1 DE19651642661 DE1642661A DE1642661A1 DE 1642661 A1 DE1642661 A1 DE 1642661A1 DE 19651642661 DE19651642661 DE 19651642661 DE 1642661 A DE1642661 A DE 1642661A DE 1642661 A1 DE1642661 A1 DE 1642661A1
Authority
DE
Germany
Prior art keywords
urokinase
sodium phosphate
carboxymethyl cellulose
production
eluted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE19651642661
Other languages
English (en)
Inventor
Alex Lesuk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STWB Inc
Original Assignee
Sterling Drug Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sterling Drug Inc filed Critical Sterling Drug Inc
Publication of DE1642661A1 publication Critical patent/DE1642661A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (1)

  1. Patentansprüche
    1.) Verfahren zur Gewinnung von Urokinase aus einer wäßrigen Lösung, die mindestens 500 CTA-Einheiten Urokinase pro ml enthält durch Absorption und Elution, dadurch gekennzeichnet, daß die wäßrige Lösung bei einem pH von 5,0 bis 6,5 mit Carboxymethylcellulose kontaktiert und anschließend die gereinigte Urokinase von der Carboxymethylcellulose eluiert wird.
    2.) Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß die molare Konzentration der Ausgangslösung an Natriumphosphat, falls Natriumphosphat vorhanden ist, vor der Zugabe der Carboxymethylcellulose auf einen Wert von nicht größer als 0,15 eingestellt wird.
    5.) Verfahren nach Anspruch 1 oder 2, dadurch gekennzeichnet, daß die Urokinase mit einem Natriumphosphatpuffer von pH 6,0 bis 7,0 und mit einer molaren Konzentration an Natriumphosphat von mindestens 0,25 eluiert wird.
    109831/0429
DE19651642661 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase Pending DE1642661A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US404156A US3355361A (en) 1964-10-15 1964-10-15 Recovery and purification of urokinase

Publications (1)

Publication Number Publication Date
DE1642661A1 true DE1642661A1 (de) 1971-07-29

Family

ID=23598395

Family Applications (4)

Application Number Title Priority Date Filing Date
DE19651642662 Pending DE1642662A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase
DE19651642660 Pending DE1642660A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase
DE19651642661 Pending DE1642661A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase
DE19651442162 Pending DE1442162A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE19651642662 Pending DE1642662A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase
DE19651642660 Pending DE1642660A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE19651442162 Pending DE1442162A1 (de) 1964-10-15 1965-10-13 Verfahren zur Gewinnung von Urokinase

Country Status (8)

Country Link
US (1) US3355361A (de)
JP (3) JPS5142195B1 (de)
AT (1) AT278239B (de)
CH (1) CH495384A (de)
DE (4) DE1642662A1 (de)
DK (4) DK117821B (de)
GB (1) GB1068817A (de)
SE (1) SE314045B (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH467075A (de) * 1964-07-01 1969-01-15 Pharmexbio S A Verfahren zur Gewinnung von Urokinase
US3477910A (en) * 1966-10-17 1969-11-11 Century Lab Inc Method of production of urokinase
US3542646A (en) * 1966-11-22 1970-11-24 Green Cross Corp Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine
US3477913A (en) * 1967-10-31 1969-11-11 Century Lab Inc Method of production of urokinase
US3544427A (en) * 1968-04-18 1970-12-01 Century Lab Inc Method of production of urokinase
US3620924A (en) * 1969-04-01 1971-11-16 Baxter Laboratories Inc Production of acid-active lactase
FR2190412A1 (en) * 1972-06-30 1974-02-01 Choay Sa Stable,pyrogen-free urokinase prepns - from human urine
BE789189A (fr) * 1971-09-24 1973-03-22 Choay Sa Perfectionnements aux procedes de preparation, de depyrogenation et de stabilisation de l'urokinase et compositions obtenues
JPS5341485A (en) * 1976-09-25 1978-04-14 Wakamoto Pharma Co Ltd Removing method of pyrogenic substance containe in urokinase
US4066506A (en) * 1976-10-08 1978-01-03 The United States Of America As Represented By The Secretary Of Health, Education And Welfare Method of separating and purifying two active forms of urokinase using affinity chromatography
JPS53142592A (en) * 1977-05-13 1978-12-12 Green Cross Corp:The Stabilization of urokinase heating
JPS53142593A (en) * 1977-12-12 1978-12-12 Green Cross Corp:The Stabilization of urokinase by heating
JPS54155784U (de) * 1978-04-21 1979-10-30
WO1981001417A1 (en) * 1979-11-13 1981-05-28 S Husain Isolation of plasminogen activators useful as therapeutic and diagnostic agents
USRE32271E (en) * 1979-11-13 1986-10-28 Isolation of plasminogen activators useful as therapeutic and diagnostic agents
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
JPS58189122A (ja) * 1982-04-30 1983-11-04 Kaken Pharmaceut Co Ltd 高脂血症予防治療剤
DE3439980A1 (de) * 1984-11-02 1986-05-07 Behringwerke Ag, 3550 Marburg Verfahren zur reinigung sowie pasteurisierung von urokinase
US4920051A (en) * 1988-02-03 1990-04-24 Damon Biotech, Inc. Recovery of urokinase compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3281331A (en) * 1961-11-08 1966-10-25 Astra Ab Process of isolating and separating proteolytic enzymes

Also Published As

Publication number Publication date
CH495384A (de) 1970-08-31
US3355361A (en) 1967-11-28
DK117821B (da) 1970-06-08
DK115536B (da) 1969-10-20
JPS5619228B1 (de) 1981-05-06
JPS5619229B1 (de) 1981-05-06
DK112162B (da) 1968-11-18
SE314045B (de) 1969-09-01
GB1068817A (en) 1967-05-17
DE1642662A1 (de) 1971-05-19
DE1442162A1 (de) 1969-01-16
AT278239B (de) 1970-01-26
JPS5142195B1 (de) 1976-11-13
DE1642660A1 (de) 1971-07-29
DK112232B (da) 1968-11-25

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