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DE10352416B4 - Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts - Google Patents

Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts Download PDF

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Publication number
DE10352416B4
DE10352416B4 DE10352416A DE10352416A DE10352416B4 DE 10352416 B4 DE10352416 B4 DE 10352416B4 DE 10352416 A DE10352416 A DE 10352416A DE 10352416 A DE10352416 A DE 10352416A DE 10352416 B4 DE10352416 B4 DE 10352416B4
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DE
Germany
Prior art keywords
positioning
methods
field
examining
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE10352416A
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English (en)
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DE10352416A1 (de
Inventor
Thomas Schnelle
Torsten Mueller
Andre Hoemke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Revvity Cellular Technologies GmbH
Original Assignee
Evotec Technologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Technologies GmbH filed Critical Evotec Technologies GmbH
Priority to DE10352416A priority Critical patent/DE10352416B4/de
Priority to PCT/EP2004/012741 priority patent/WO2005045400A1/de
Priority to US10/595,771 priority patent/US20070119714A1/en
Priority to EP04797789A priority patent/EP1682868A1/de
Publication of DE10352416A1 publication Critical patent/DE10352416A1/de
Application granted granted Critical
Publication of DE10352416B4 publication Critical patent/DE10352416B4/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1022—Measurement of deformation of individual particles by non-optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G01N2015/1495—Deformation of particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Es werden Verfahren zur Untersuchung von mindestens einem deformierbaren Objekt (O) in einer Suspensionsflüssigkeit beschrieben, mit den Schritten: Erzeugung eines elektrischen Positionierungs-Feldes und Positionierung des Objekts (O) in einem Potentialminimum des Positionierungs-Feldes, Erzeugung eines elektrischen Deformations-Feldes, derart, dass eine Deformationskraft auf das Objekt (O) ausgeübt wird, und Detektion von mindestens einer Eigenschaft aus der Gruppe der dielektrischen, geometrischen und optischen Eigenschaften des Objekts (O), wobei das Positionierungs-Feld in einem Kompartiment (12) eines fluidischen Mikrosystems (10) erzeugt wird, und die Positionierung des Objekts (O) in frei suspendiertem Zustand berührungslos erfolgt. Es werden auch Messapparaturen zur Durchführung dieser Verfahren beschrieben.
DE10352416A 2003-11-10 2003-11-10 Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts Expired - Fee Related DE10352416B4 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE10352416A DE10352416B4 (de) 2003-11-10 2003-11-10 Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts
PCT/EP2004/012741 WO2005045400A1 (de) 2003-11-10 2004-11-10 Verfahren und vorrichtungen zur untersuchung eines deformierbaren objekts
US10/595,771 US20070119714A1 (en) 2003-11-10 2004-11-10 Methods and devices for analysing a deformable object
EP04797789A EP1682868A1 (de) 2003-11-10 2004-11-10 Verfahren und vorrichtungen zur untersuchung eines deformierbaren objekts

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10352416A DE10352416B4 (de) 2003-11-10 2003-11-10 Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts

Publications (2)

Publication Number Publication Date
DE10352416A1 DE10352416A1 (de) 2005-06-16
DE10352416B4 true DE10352416B4 (de) 2005-10-20

Family

ID=34559545

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10352416A Expired - Fee Related DE10352416B4 (de) 2003-11-10 2003-11-10 Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts

Country Status (4)

Country Link
US (1) US20070119714A1 (de)
EP (1) EP1682868A1 (de)
DE (1) DE10352416B4 (de)
WO (1) WO2005045400A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE285590T1 (de) * 2002-10-25 2005-01-15 Evotec Technologies Gmbh Methode und vorrichtung zur aufnahme dreidimensionaler abbildungen von schwebend gehaltenen mikroobjekten unter verwendung hochauflösender mikroskopie
US7542131B2 (en) * 2003-06-23 2009-06-02 Sewon Meditech, Inc. Apparatus for measuring blood cell deformability
DE102004017474A1 (de) * 2004-04-08 2005-10-27 Evotec Technologies Gmbh Messeinrichtung zur Impedanzspektroskopie und zugehöriges Messverfahren
DE102006052925A1 (de) * 2006-11-09 2008-05-15 Evotec Technologies Gmbh Feldkäfig und zugehöriges Betriebsverfahren
US9126198B2 (en) * 2009-02-20 2015-09-08 Japan Science And Technology Agency Transportation of micrometer-sized object and extraction of mechanical work by constant electric field
DE102010023099B3 (de) * 2010-06-09 2011-11-17 Celltool Gmbh Verfahren und Vorrichtung zum Charakterisieren von biologischen Objekten
WO2015038998A1 (en) * 2013-09-13 2015-03-19 The Administrators Of The Tulane Educational Fund Apparatus, systems & methods for non-contact rheological measurements of biological materials
DE102018213965A1 (de) * 2018-08-20 2020-02-20 Universität Ulm Vorrichtung und Verfahren zur Detektion einer räumlichen Streckung von mindestens einer adhärenten biologischen Zelle

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4434883A1 (de) * 1994-02-24 1995-08-31 Stefan Fiedler Formen von Mikropartikeln in elektrischen Feldkäfigen
DE19903001A1 (de) * 1999-01-26 2000-08-24 Evotec Biosystems Ag Verfahren und Vorrichtung zur Detektion mikroskopisch kleiner Objekte

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525666A (en) * 1982-05-03 1985-06-25 Coulter Electronics, Inc. Cell breakdown
US4970154A (en) * 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
EP0746408B1 (de) * 1994-02-24 1998-09-23 Fraunhofer-Gesellschaft Zur Förderung Der Angewandten Forschung E.V. Formen von mikropartikeln in elektrischen feldkaefigen
DE19859459A1 (de) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Mikrosysteme zur Zellpermeation und Zellfusion
US6067859A (en) * 1999-03-04 2000-05-30 The Board Of Regents, The University Of Texas System Optical stretcher
FR2831084B1 (fr) * 2001-10-22 2004-08-27 Centre Nat Rech Scient Procede et systeme pour manipuler par dielectrophorese des particules dielectriques, en particulier des cellules biologiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4434883A1 (de) * 1994-02-24 1995-08-31 Stefan Fiedler Formen von Mikropartikeln in elektrischen Feldkäfigen
DE19903001A1 (de) * 1999-01-26 2000-08-24 Evotec Biosystems Ag Verfahren und Vorrichtung zur Detektion mikroskopisch kleiner Objekte

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
H.Engelhardt et al.: Viscoelastic properties of erythrocyte membranes in high frequency electric fields, Nature, Bd.307, 1987, S.378-380 *
K.J.Müller et al.: Reversible Electropermeabili- zation of mammalian cells by high-intensity, ul- tra short pulses of submicrosecond duration, J.Membrane Biol., Bd.184, 2001, S.161-170 *
T.Schnelle et al.: Trapping in ac octode field cages, J.Electrostat., Bd.50, 2000, S.17-29 *
Viren und Bakterien berührungslos handhaben - ein Lab-on-a-Chip-System mit integrierten 3D-Nanoelek-trodenarrays, VDI-Technologiezentrum, Info Phys Tech Nr. 42 *

Also Published As

Publication number Publication date
DE10352416A1 (de) 2005-06-16
US20070119714A1 (en) 2007-05-31
WO2005045400A1 (de) 2005-05-19
EP1682868A1 (de) 2006-07-26

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