DE1008867B - Process for obtaining salt-free, easily soluble dry preparations of proteins, in particular globulins - Google Patents
Process for obtaining salt-free, easily soluble dry preparations of proteins, in particular globulinsInfo
- Publication number
- DE1008867B DE1008867B DESCH20229A DESC020229A DE1008867B DE 1008867 B DE1008867 B DE 1008867B DE SCH20229 A DESCH20229 A DE SCH20229A DE SC020229 A DESC020229 A DE SC020229A DE 1008867 B DE1008867 B DE 1008867B
- Authority
- DE
- Germany
- Prior art keywords
- proteins
- globulins
- free
- easily soluble
- dry preparations
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B3/00—Drying solid materials or objects by processes involving the application of heat
- F26B3/02—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air
- F26B3/10—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air the gas or vapour carrying the materials or objects to be dried with it
- F26B3/12—Drying solid materials or objects by processes involving the application of heat by convection, i.e. heat being conveyed from a heat source to the materials or objects to be dried by a gas or vapour, e.g. air the gas or vapour carrying the materials or objects to be dried with it in the form of a spray, i.e. sprayed or dispersed emulsions or suspensions
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Microbiology (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Description
Verfahren zur Gewinnung salzfreier, leicht löslicher Trockenpräparate von Proteinen, insbesondere Globulinen Im Hauptpatent 959 127 ist ein Verfahren zur Herstellung salzarmer, leicht löslicher Trockenpräparate von Proteinen, insbesondere Globulinen, auf dem Wege der Gefriertrocknung salzhaltiger wäßriger Lösungen der Proteine beschrieben, welches dadurch gekennzeichnet ist, daß man von solchen wäßrigen Proteinlösungen ausgeht, die nur Salze hoher Flüchtigkeit, wie Ammoniumcarbonat, Ammoniumformiat, Ammoniumacetat u. dgl., gegebenenfalls mit Zusätzen flüchtiger Basen, wieArnmoniak, oder flüchtiger Säuren, wie Ameisensäure, Essigsäure usw., zwecks Auspufferung auf ein gewünschtes PH enthalten.Process for obtaining salt-free, easily soluble dry preparations of proteins, especially globulins. Main patent 959 127 is a process for the production of low-salt, easily soluble dry preparations of proteins, in particular Globulins, by freeze-drying saline aqueous solutions of the Proteins described, which is characterized in that one of such aqueous Runs out of protein solutions that only contain salts of high volatility, such as ammonium carbonate, Ammonium formate, ammonium acetate and the like, optionally with volatile additives Bases, such as ammonia, or volatile acids, such as formic acid, acetic acid, etc., included for the purpose of buffering to a desired PH.
In Abänderung des Verfahrens des Hauptpatents wurde nun gefunden, daß man zu gleichwertigen Protein-Trockenpräparaten gelangt, wenn man die Ausgangslösungen des Hauptpatents statt der Gefriertrocknung einer Sprühtrocknung bei tunlichst schonender Temperatur, erforderlichenfalls bei entsprechend erniedrigtem Druck, unterwirft.In an amendment to the process of the main patent, it has now been found that one arrives at equivalent protein dry preparations if one uses the starting solutions of the main patent, instead of freeze-drying, spray-drying with the greatest possible care Temperature, if necessary at a correspondingly reduced pressure, subjected.
Auch mittels dieser technisch einfacheren. Trocknungsmet!hode läßt sich der Salzgehalt der Ausgangslösungen mit Leichtigkeit praktisch völlig beseitigen, und man erhält die Proteine als lockeres Trockenpulver von guter Wiederauflösbarkeit. Letztere läßt sich mitunter noch wesentlich verbessern, wenn man der Ausgangslösung in geringer Menge ein Netzmittel, wie das unter dem Markennamen »Tween 80« im Handel befindliche, zusetzt.Even by means of this technically simpler one. Drying method the salt content of the starting solutions is almost completely eliminated with ease, and the proteins are obtained as a loose dry powder with good redissolubility. The latter can sometimes be improved significantly if you use the initial solution a small amount of a wetting agent, such as the one under the brand name »Tween 80« located, clogs.
Beispiel l 51 frisches Rinderserum werden unter Bewegung gegen 0,1 mol. Ammonbikarbonatpuffer (etwa PH 7,5) dialysiert. Das klare dialysierte Serum wird unter schonenden Bedingungen sprühgetrocknet. Es wird hierbei ein Pulver erhalten, das praktisch frei von Salzen ist. Das Pulver (Globuline) ist nicht löslich in destilliertem Wasser, vollständig löslich dagegen in physiologischer Kochsalzlösung.Example 1 51 fresh bovine serum are agitated against 0.1 mol. Dialyzed ammonium bicarbonate buffer (approx. PH 7.5). The clear dialyzed serum is spray-dried under gentle conditions. A powder is obtained here, which is practically free of salts. The powder (globuline) is not soluble in distilled Water, on the other hand completely soluble in physiological saline solution.
Beispiel 2 201 einer 1d/oigen Papainlösung werden zur Ausfällung von Ballastproteinen mit Ammon.i-umsulfat gesättigt. Das klare Filtrat, enthaltend Enzym und 6 kg Ammoniumsulfat wird unter Rühren und Kohlensäure-Einleiten bei -3' mit 8,4 kg Bariumsulfat versetzt. Nach Absirtzen des gebildeten Bariumsulfats wird dekantiert und zentrifugiert.Example 2 201 of a 1% papain solution are saturated with ammonium sulfate to precipitate ballast proteins. The clear filtrate, containing enzyme and 6 kg of ammonium sulfate, is mixed with 8.4 kg of barium sulfate while stirring and passing in carbonic acid at -3 '. After the barium sulfate formed has evaporated, it is decanted and centrifuged.
Die klare Lösung wird im Vakuum bei 30 bis 35° InnentenperatuT auf 4 bis 5 1 eingeengt und enthält dann nur noch etwa 1 kg Ammoniumcarlxinat. Während des Einengens erfolgt keine Ausfällung des Proteins. (Wenn erwünscht, kann der Salzgehalt durch Zugabe von etwa 10 1 Wasser und nochmaliges Einengen unter den gleichen Bedingungen auf 500 g und darunter heruntergedrückt werden.) Dieser Papainlösung werden zweckmäßig als Netzmittel einige Tropfen »Tween 80« zugesetzt.The clear solution is in a vacuum at 30 to 35 ° internal temperature 4 to 5 1 concentrated and then contains only about 1 kg of ammonium carbonate. While after concentration, there is no precipitation of the protein. (If desired, the salinity can be by adding about 10 l of water and concentrating again under the same conditions be pressed down to 500 g and below.) This papain solution will be useful A few drops of "Tween 80" were added as a wetting agent.
Die Lösung wird sprühgetrocknet. Das anfallende Papainpulver ist in destilliertem Wasser löslich und hat nichts an biologischer Aktivität (Einheiten/mg) verloren. Die Lösungsgeschwindigkeit des sprühgetrockneten Pulvers wird hier durch das Netzmittel besonders günstig beei,nflußt.The solution is spray dried. The resulting papain powder is in soluble in distilled water and has no biological activity (units / mg) lost. The speed of dissolution of the spray-dried powder is here through the wetting agent is particularly beneficial.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DESCH20229A DE1008867B (en) | 1956-06-09 | 1956-06-09 | Process for obtaining salt-free, easily soluble dry preparations of proteins, in particular globulins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DESCH20229A DE1008867B (en) | 1956-06-09 | 1956-06-09 | Process for obtaining salt-free, easily soluble dry preparations of proteins, in particular globulins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1008867B true DE1008867B (en) | 1957-05-23 |
Family
ID=7428697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DESCH20229A Pending DE1008867B (en) | 1956-06-09 | 1956-06-09 | Process for obtaining salt-free, easily soluble dry preparations of proteins, in particular globulins |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE1008867B (en) |
-
1956
- 1956-06-09 DE DESCH20229A patent/DE1008867B/en active Pending
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