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DE10010281B4 - Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben - Google Patents

Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben Download PDF

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Publication number
DE10010281B4
DE10010281B4 DE10010281A DE10010281A DE10010281B4 DE 10010281 B4 DE10010281 B4 DE 10010281B4 DE 10010281 A DE10010281 A DE 10010281A DE 10010281 A DE10010281 A DE 10010281A DE 10010281 B4 DE10010281 B4 DE 10010281B4
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DE
Germany
Prior art keywords
ligase
detection
dna samples
cytosine methylation
polymerase method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE10010281A
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English (en)
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DE10010281A1 (de
Inventor
Alexander Olek
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE10010281A priority Critical patent/DE10010281B4/de
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to AU2001242279A priority patent/AU2001242279B2/en
Priority to JP2001561769A priority patent/JP2003523752A/ja
Priority to AU4227901A priority patent/AU4227901A/xx
Priority to CA002401198A priority patent/CA2401198A1/en
Priority to US10/204,961 priority patent/US7405040B2/en
Priority to PCT/DE2001/000749 priority patent/WO2001062961A1/de
Priority to EP01915053A priority patent/EP1261740A1/de
Publication of DE10010281A1 publication Critical patent/DE10010281A1/de
Application granted granted Critical
Publication of DE10010281B4 publication Critical patent/DE10010281B4/de
Priority to AU2006236059A priority patent/AU2006236059A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
DE10010281A 2000-02-25 2000-02-25 Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben Expired - Fee Related DE10010281B4 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE10010281A DE10010281B4 (de) 2000-02-25 2000-02-25 Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
JP2001561769A JP2003523752A (ja) 2000-02-25 2001-02-23 Ｄｎａプローブにおいてシトシンのメチル化を検出するリガーゼ／ポリメラーゼ法
AU4227901A AU4227901A (en) 2000-02-25 2001-02-23 Ligase/polymerase method for detecting cytosine methylation in dna samples
CA002401198A CA2401198A1 (en) 2000-02-25 2001-02-23 Ligase/polymerase method for detecting cytosine methylation in dna samples
AU2001242279A AU2001242279B2 (en) 2000-02-25 2001-02-23 Ligase/polymerase method for detecting cytosine methylation in DNA samples
US10/204,961 US7405040B2 (en) 2000-02-25 2001-02-23 Ligase/polymerase method for detecting cytosine methylation in DNA samples
PCT/DE2001/000749 WO2001062961A1 (de) 2000-02-25 2001-02-23 Ligase/polymerase-verfahren zur detektion von cytosin-methylierung in dna proben
EP01915053A EP1261740A1 (de) 2000-02-25 2001-02-23 Ligase/polymerase-verfahren zur detektion von cytosin-methylierung in dna proben
AU2006236059A AU2006236059A1 (en) 2000-02-25 2006-11-17 Ligase/polymerase method for detecting cytosine methylation in DNA samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10010281A DE10010281B4 (de) 2000-02-25 2000-02-25 Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben

Publications (2)

Publication Number Publication Date
DE10010281A1 DE10010281A1 (de) 2001-09-06
DE10010281B4 true DE10010281B4 (de) 2005-03-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE10010281A Expired - Fee Related DE10010281B4 (de) 2000-02-25 2000-02-25 Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben

Country Status (7)

Country Link
US (1) US7405040B2 (de)
EP (1) EP1261740A1 (de)
JP (1) JP2003523752A (de)
AU (3) AU4227901A (de)
CA (1) CA2401198A1 (de)
DE (1) DE10010281B4 (de)
WO (1) WO2001062961A1 (de)

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US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
DE10010280B4 (de) * 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10154318A1 (de) * 2001-10-26 2003-05-15 Epigenomics Ag Verfahren zur Analyse genomischer Methylierungsmuster
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1860200A1 (de) * 2001-12-03 2007-11-28 Illumina, Inc. Multiplexverfahren zur Methylierungsdetektion
DE10201138B4 (de) * 2002-01-08 2005-03-10 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
DE60331852D1 (de) * 2002-12-02 2010-05-06 Illumina Cambridge Ltd Bestimmung der methylierung von nukleinsäuresequenzen
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
DE602004028478D1 (de) * 2003-06-17 2010-09-16 Human Genetic Signatures Pty Verfahren zur genomamplifikation
DE602004018801D1 (de) * 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nukleinsäurenachweistest
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
ATE476439T1 (de) * 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
NZ555620A (en) * 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
AU2005318874B2 (en) * 2004-12-23 2007-05-03 Human Genetic Signatures Pty Ltd Detection of human papilloma virus
WO2006113770A1 (en) 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample
CA2609218C (en) * 2005-05-26 2016-10-11 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP1943353B1 (de) * 2005-09-14 2011-11-02 Human Genetic Signatures PTY Ltd Gesundheitszustandstest
EP3095878A1 (de) 2005-11-17 2016-11-23 Epigenomics AG Verfahren zur bestimmung des dna-methylierungsgrads einer cpg-position in identischen zellen in einer gewebeprobe
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8084734B2 (en) * 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20100092972A1 (en) * 2007-03-16 2010-04-15 Human Genetic Signatures Pty Ltd. Assay for gene expression
EP2152902A2 (de) 2007-06-08 2010-02-17 Epigenomics AG Verfahren zur methylierungsanalyse
JP5431351B2 (ja) * 2007-11-27 2014-03-05 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド バイサルファイト修飾された核酸を増幅およびコピーするための酵素
EP2222850A4 (de) * 2007-12-20 2011-12-07 Human Genetic Signatures Pty Beseitigung von kontaminanten in zusammenhang mit nukleinsäureamplifikation
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
US8110796B2 (en) 2009-01-17 2012-02-07 The George Washington University Nanophotonic production, modulation and switching of ions by silicon microcolumn arrays
US9490113B2 (en) * 2009-04-07 2016-11-08 The George Washington University Tailored nanopost arrays (NAPA) for laser desorption ionization in mass spectrometry
EP2309005B1 (de) 2009-08-03 2015-03-04 Epigenomics AG Verfahren zur Konservierung von genomischer DNA-Sequenzkomplexität
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
SG11201400217WA (en) 2011-09-07 2014-03-28 Human Genetic Signatures Pty Molecular detection assay
JP2023516084A (ja) 2020-03-03 2023-04-17 コーデックス ディーエヌエー インコーポレイテッド 核酸をアセンブルするための方法

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US5728526A (en) * 1995-06-07 1998-03-17 Oncor, Inc. Method for analyzing a nucleotide sequence
WO1999067414A1 (en) * 1998-06-24 1999-12-29 Glaxo Group Limited Nucleotide detection method

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US6013431A (en) * 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
AU694187B2 (en) * 1994-02-07 1998-07-16 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis
EP0912761A4 (de) * 1996-05-29 2004-06-09 Cornell Res Foundation Inc Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
DE10010280B4 (de) * 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10029915B4 (de) * 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US5728526A (en) * 1995-06-07 1998-03-17 Oncor, Inc. Method for analyzing a nucleotide sequence
WO1999067414A1 (en) * 1998-06-24 1999-12-29 Glaxo Group Limited Nucleotide detection method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SnuPE). Gonzalgo, M.L. & Jones, P.A., Nucleic Acid Res. (1997)25, 2529-2531 *

Also Published As

Publication number Publication date
CA2401198A1 (en) 2001-08-30
AU2001242279B2 (en) 2006-10-26
US7405040B2 (en) 2008-07-29
WO2001062961A1 (de) 2001-08-30
EP1261740A1 (de) 2002-12-04
US20030119025A1 (en) 2003-06-26
DE10010281A1 (de) 2001-09-06
AU4227901A (en) 2001-09-03
AU2006236059A1 (en) 2006-12-07
JP2003523752A (ja) 2003-08-12

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R082 Change of representative

Representative=s name: DR. VOLKER VOSSIUS PATENT- UND RECHTSANWALTSKA, DE

Representative=s name: ZSP PATENTANWAELTE PARTNERSCHAFTSGESELLSCHAFT, DE

Representative=s name: ZSP PATENTANWAELTE PARTG MBB, DE

R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee