CY1876A

CY1876A - Preparation of antihypercholesterolemic tetrazole intermediates

Info

Publication number: CY1876A
Application number: CY187696A
Authority: CY
Original assignee: Bristol Myers Squibb Co
Priority date: 1988-02-18
Filing date: 1996-04-05
Publication date: 1996-04-05
Also published as: GB9105304D0; CY1875A; GB2244706B; GB9105305D0; GB2244706A; GB2244705B; GB2244705A

Description

PREPARATION OF A\TlHYFERCHOLESTEROLE*ttC TETRAZQLE OT.POUDS A\D 1NTERMEDIATE5 THEREOF

SUMMARY OF THE IN"YENT3 CN

The present inventir-n provides novel intermediates r.rd processes for the preparation of ccrpcuncs cf the

R' R10 tr^o o

•-!£

Ilia

9 io 12

in substantially the cis form vherein R , R and R. are as defined below which are useful for the preparation of inhibitors of the enzyme 3-hydroxy-3-nethylglutaryl coenzyme A (HMG-CoA) reductase, and therefore, are useful in the treatment of hypercholesterolemia, hyperlipoproteinemia and atherosclerosis. The present invention also provides a simple and convenient chiral process for the preparation of inhibitors of HMG-CoA reductase and to certain chiral intermediates thereof.

-2-

DESCRIPTICN OF THE INDENTION

The present invention provides intermediates which are useful for the preparation of antihyperchclesterolemic agents, and which have the formulae

X

R10

= 12

OR

Ilia in substantially the cis form wherein

Illb

R9 and R10

, 12

.10

each are C^_^alkyl or R anc R", taken together with the carbon atom to which they are attached, is cyclopentyl, cyclohexyl or cycloheptyl; and is hydrogen, C^_^alkyl or a metal cation.

This invention also provides intermediates and processes for the preparation of the compounds of Formulae Ilia and Illb and to intermediates and processes for the preparation of antihypercholesterolemic agents of the formulae R5

OH 0

II

-3-

BNSDOCID: <GB 2244706A L>

wherein R and R each ere independently hydrogen, halogen, C^^alkyl, C^^alkoxy or trifluoromethyl; , R3, R5 and R6 each are independently hydrogen, halogen, ^alkyl or C^_^alkoxy; and R is hydrogen, a hydrolyzable ester group or a cation to form a non-toxic pharmaceutical^ acceptable salt.

The teras "C^^alky 1", "C^_g£lkyl" and "C^_^alkoxy" as used herein and in the claims (unless the context indicates otherwise) mean unbranched or branched chain alkyl or alkoxy groups such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, amyl, hexyl, etc. Freferably, these groups contain from 1 to 4 carbon atoms and, most preferably, they contain 1 or 2 carbon atoms. Unless otherwise specified in the particular instance, the term , "halogen" as used herein and in the claims is intended to include chlorine, fluorine, bromine and iodine while the term "halide" as used herein and in the claims is intended to include chloride, bromide and iodide anion. The term "a cation to form a non-toxic pharmaceutical^ acceptable salt" as used herein and in the claims is intended to include non-toxic alkali metal salts such as sodium, potassium, calcium and magnesium, the ammonium salt and salts with non-toxic amines such as trialkylamines, dibenzylamine, pyridine, N-methylmorpholine, N-methylpiperidine and other amines which have been used to form salts of carboxylic

-4-

BNSDOCID: <GB 2244706A l_>

acids. Unless otherwise specified, the term "a hydrolyzable ester group" as used herein and in the claims is intended to include an ester group which is physiologically acceptable and hydrolyzable under physiological conditions such as C^_6alkyl, phenylmethyl and pivaloyloxymethyl.

In the compounds of formulae I, II, XI and XII, it is intended that the double bonds are in the trans configuration, i.e., (E), as indicated in the structural formulae used herein and in the claims. VThereas in the compounds of Formulae IV, V, VI, VII, VIII and IX, it is intended that the configuration of the double bends are trans, cis or mixtures thereof, i.e., (E), (Z) when n=o and (E)(E), (Z)(Z), (E)(Z) and (Z)(E) when n=l, as indicated herein and in the claims.

As the compounds of the present invention possess two asymmetric carbon atoms, the invention includes the enantiomeric and diastereorneric forms of the intermediates utilized in the processes for the preparation of compounds of Formulae I and II as described herein and in the claims. The compounds of Formulae I and II which contain two centers of asymmetry may have four possible stereoisomers designated as the RR, RS, SR and SS enantiomers. Specifically, the compounds of Formula I having two asymmetric carbon atoms bearing the hydroxy groups in the 3 and 5 position may have four possible stereoisomers which are designated as the

t

(3R,5S), (3S,5R), (3R,5R) and (3S,5S) stereoisomers. As used herein and in the claims, the term "erythro" is intended to include a mixture of (3R,5S) and (3S,5R) enantiomers, and the term "threo" is intended to include a mixture of (3R.5R) and (3S,5S) enantiorr.ers. The use of single designation such as (3R,5S) is intended to include substantially one stereoisomer. The lactone compounds of Formula II also have two asymmetric carbon atoms at the 4 and 6 position, and the resulting four stereoisomers may be designated as the (4R,6S), (4S,6R), (4R,6R) and (^S,6S) stereoisomers. As used herein and in the claims, the term "trans" lactone is intended to include a mixture cf (4R,6S) and (4S,6R) enantiomers while the term "cis" lactone is intended to include a mixture of (4R,6R) and (4S,6S) enantiomers. The use of a single designation such as (4R,6S) is intended to include substantially one enantiomeric lactone.

The substituted 1,3-dioxane compounds of Formula Ilia, Illb and other similar compounds described herein and in the claims also contain two asymmetric carbon atoms at the 4 and 6 position as shown below,

X

-6-

BNSDOCJD: <GB 2244706A |_>

and the resulting four stereoisomers may be designated as the (4R,6S), (4S,6R), (4R,6R) and (4S,6S) stereoisomers. As used herein and in the claims, the term "trans" - l,3-dio>;ane is intended to include a mixture of (4R,6R) and (4S,6S) enantiomers while the term "cis"-1.3-dioxane is intended to include a mixture of (4R,6S) and (4S,6R) enantiomers. Since the most preferred enanticmer of the lactone compounds of Formula II has fortuitously the same (4R.,6S) stereoiscmeric designation as the most preferred enantiomer of the 1,3-dioxane intermediates of the present invention, the additional designation of "trans" or "cis" is included to avoid any possible confusion.

9

In the compounds of Formulae Ilia and Illb, R and R^ each are C^_^alkyl or R9 and R^, taken together ; with the carbon atom to which they are attached, is

9

cyclopentyl, cyclohexyl or cycloheptyl. Freferably, R and each are methyl or R^ and R^, taken together with the carbon atom to which they are attached, is cyclohexyl. It

12

is preferred that R is hydrogen, methyl or a metal cation especially lithium. The cis isomer of the compounds of Formula Ilia is preferred and the cis-(4R,6S) isomer of the compounds of Formula Illb is most preferred.

The antihypercholesterolemic compounds of Formulae I and II may be prepared by various procedures and preferably

-7-

BNSDOCID: <rGB 2244706A__I„>

by employing the intermediates of the formulae

9 10 12

in substantially the cis form wherein R , R and R are as defined previously. Thus, the present invention provides a process for the preparation of the intermediates of Formulae Ilia and Illb and also provides an irr.prcved processs for the preparation of compounds of the Formulae I and II.

The compounds of Formulae Ilia and Illb may be prepared by the reaction of an aldehyde of Formula IV with an ester of acetoacetic acid and then reacting a ketone or ketal vith a compound of Formula VI followed by hydrolysis of the resulting 1,3-dicxane of Formula VII and optionally resolving the acid of Formula VIII, as shown in Reaction Scheme 1.

-8-

BNSDOCID: <GB 2244706A_I_>

Reaction Scheme 1

1 A

+ C H-

0 0

p-1 1

U(\

IV

OH 0 0

CR

U

V

UK

11

VI

9 D10

R8

X

CR11

VII

p 9 d 1 0

cr^o

(^N

VIII

RV .R10

><

0 0

OR

1£

Ilia

R9 DIO

X

IX

}V*5

ir^o

3? ^10

'0 o

OR

ie

Illb

-9-

BNSDOCID: <GB 2244706A_L>

7 8

In Reaction Scheme 1, R and R each ara independently hydrogen, C^_^alkyl cr phenyl which is optionally substituted by one or two C^_^alkyl, halogen,

^alkoxy or trif luoromethyl; R^ is a hydrolyzable ester

9 10

group, n is zero or 1 and R and R are as previously defined. The ketoester of Formula V may be prepared by the reaction of an ester of acetoacetic'acid with an aldehyde of Formula IV by procedures well-known to those skilled in the art in an inert organic solvent such as tetrahydrcfuran at temperatures of about 0°C to about -78°C in the presence of a base such as sodium hydride, lithium diisoprcpylamide and n-butyllithium.

The starting materials of Formula IV wherein n=0 and n=l are known or may readily be prepared by known methods. The starting materials of Formula IV wherein n=l may also be prepared by the reaction of compounds of Formula IV wherein n=0 with Vittig reagents such as triphenylphos-phoranylidene acetaldehyde and other methods well-known in the art. It should be appreciated by those skilled in the art that the relative configuration of the double bond (n=0) or double bonds (n=l) in the starting materials of Formula IV may be trans, cis or mixtures thereof. The relative amounts of each geometric isomer (E) or (Z) will be determined by commercial availability or the reaction conditions employed in the preparation. In a specific example described herein, a mixture containing mostly trans

-10-

(E) isomer was employed. Even though a small percent of the other isomer may be present throughout the series of reactions shown in Reaction Scheme 1, it should be evident to those skilled in the art that the relative amount of isomers is not critical since the double bond is oxidized and thereby removed in the ozonolys is re act ion.

The ketoester of Formula V may be reduced to the dihydroxyester of Formula VI by reduction of the ketone group with reducing agents well-known in the art.

Preferably, the reduction is carried out in a stereospecific manner by a two-step stereospecific reduction in order to maximize the production of the preferred ervthro isomer of the dihydroxyester of Formula VI. The stereospecific reduction is carried out with trisubstitutedalkylboranes, preferably triethyIborane or tri-n-butylborane, or alkoxydialkyIboranes, preferably methoxydiethylborane or ethoxydiethyIborane TTetrahedron Letters. 28. 155 ( 1987 )] at a temperature of about -70°C to about ambient temperature. The complex which is produced is then reduced with sodium borohydride at a temperature of about -50°C to about -78°C in an inert organic solvent such as tetrahydrofuran, diethylether and 1,2-dimethoxyethane, preferably tetrahydrofuran. The reduction is then completed by the addition of methanol with or without the addition of aqueous hydrogen peroxide and buffer. Some of the compounds of Formula VI are known and described in U.S. Patent No.

-II-

4.248.889 (issued February 3, 1981) and U.S. Patent No.

4.650.890 (issued March 17, 1987).

The compounds of Formula VII may be prepared from the compounds of Formula VI by reacting a ketone such as 2-propanone, 3-pentanone, cyclopentancne and cyclohexar.one in a suitable inert organic solvent, e.g. toluene, ber.zene or xylene at tenperatures of about 20°C to the reflux temperature of the solvent employed in the presence of a small amount'of organic, mineral or resin acid, e.g., p-toluenesulfonic acid and sulfuric acid and optionally removing the water which is formed with a drying agent, e.g., Na2S0^, KgSO^ and molecular sieves or by azeotropical removal with a Dean-Stark trap or similar apparatus. The reaction of a compound of Formula VI with a ketone may also be carried out without solvent. Alternatively, the reaction described above of compounds of Formula VII may be carried out with a ketal such as 2,2-dimethoxypropane, 1,1-dimeth-oxycyclohexane and the like.

12

The compounds of Formula Ilia wherein R is a hydrolyzable ester group, and preferably, C^_^alkyl may be prepared from the corresponding compounds of Formula VII by oxidation of the olefinic group to an aldehyde group using conventional means. Alternatively, a compound of Formula VII is first hydrolyzed by basic hydrolysis to a compound of Formula VIII which is then oxidized to give a compound of

-12-

BNSDOCID: <GB 2244706A_I_>

12

Formula Ilia wherein R is hydrogen. A particularly convenient oxidation method is the reaction of a compound of Formula VII or VIII in an inert organic solvent such as methanol, ethyl acetate and methylene chloride with ozcne at temperatures of about -50°C to about -78°C. When the reaction with ozone is complete as evidence by the color of the reaction mixture, the intermediate ozonide is decomposed by the addition of a mild reducing agent, e.g., dimethyl sulfide and triphenyIphosphine to give the desired aldehyde of Formula IIla.

The preferred cis-(4R,6S) aldehydes of Formula Illb may be prepared from the corresponding racemic acid of Formula VIII by conventional resolution methods such as fractional crystallization after the introduction of a suitable salt-forming group. The resulting mixture of diastereoisor.eric salts which is formed with an optically active salt-forming agent such as (IS,2R)-ephedrine and a-methyIbenzylamine is separated and the separated resolved salt is converted to a compound of Formula Illb.

Preferably, the salt-forming agent is (IS,2R)-ephedrine and the method of separation is by fractional crystallization. The resolution may be carried out in an inert organic solvent, and preferably, in a mixture of hydro carbon-alcohol solvents, e.g., hexane-methanol mixture, in which the resolved salt may crystallize from the solution. If it is desired, the acid of Formula Illb may be converted to a salt

-13-

vherein R is a metal cation or to a hydrolyzable ester 12

group wherein R is C^_^alkyl.

The preferred antihypercholesterolemic compounds . of Formulae I and II may be prepared from a compound of Formula Ilia or Illb by the general procedures described herein, in U.S. Patent Application Serial No. 018,542, filed February 25, 1987 and the corresponding ccntinuaticn-in-part

U.S. Patent Application Serial No. (CT-1SS8A),

filed (concurrently) by John J. Wright and Sing-Yuen

Sit, and in the U.S. Patent Application Serial No. 018,558, filed February 25, 1987 and the corresponding continuation-in-part U.S. Patent Application Serial No. -

(CT-1890A), filed concurrently by John J.

Wright, Sing-Yuen Sit, Neelakantan Balasubramanian and Peter J. Brown. The use of the aldehydes of Formula Ilia is shown in Reaction Scheme 2 and the use of the chiral aldehydes of Formula Illb is shown in Reaction Scheme 3.

-14-

Reaction Scheme 2

R5

Compound of rorinula II

-15-

BNSDOCID: <GB 2244706A_I_>

•Reaction Scheme 3

BNSDOCID: <GB 2244706A_I_>

1 n T / c

In Reaction Schemes 2 and 3,R,R,R,R,R, 0 12

and R are as previously defined and Z is

!!

— P— (0RiJ)2 or

0

or —P — R1^ X® in which

13 14

R is ^alkyl, R is phenyl which is unsubstituted or substituted by one or two C^_^alkyl or chloro substituents and X is bromo, chloro or iodo. The phosphonium salt of

Formula X and the phosphonate of Formula X is described herein, in U.S. Patent Application Serial No. 013,558, filed

February 25, 1987 and in the continuation-in-part U.S.

Patent Application Serial No. (CT-1890A), filed

(concurrently) by one of us and colleagues Sing-Yuen Sit,

Neelakantan Balasubramanian and Peter J. Brown. The reaction of a compound of Formula X with a compound of

Formula Ilia or Formula Illb to produce a compound of

12

Formula XI or XII, respectively, wherein R is C^^alkyl may be carried out in an inert organic solvent such as tetrahydrofuran and N,N-dimethylformamide in the presence of a strong base such as n-butyllithium at a temperature of about -50°C to about -78°C. When the reaction of a compound of Formula X is carried out with a compound of Formula Ilia

12

or Illb wherein R is hydrogen, it is preferred to use two equivalents of a strong base such as n-butyllithium. Alternatively, the salt of a compound of Formula Ilia or Illb may be prepared which is then treated with a compound -of Formula X and a strong base. The methods of addition,

-17-

BNSDOCID: <GB 2244706A

salt formation and ylide preparation are well-known to those skilled in the art. The tetrazole compounds of Formula XI or XII may be readily deprotected by well-known procedures such as mild acid, e.g., 0.2" HC1 and 0.5N HC1 in an inert organic solvent such as tetrahydrofuran to produce the erythro compounds of Formula la or the (3R,5S) compounds of Formula lb which may then be converted to the trans compounds of Formula II or (4R.6S) compounds of Formula II in a conventional manner we 11-known to those skilled in the art.

In a preferred embodiment of the invention, the compounds of Formula Ilia have the structure

Ilia o

9 10

in substantially the cis form wherein R and R each are 9 10

Cj^alkyl or R and R , taken together with the carbon atom

12

to which they are attached, is cyclohexyl; and R is hydrogen, C^_2alkyl or a metal cation.

-18-

BNSDOCID: <GB 2244706A_I_>

In a more preferred embodiment of the invention, the compounds of Formula Illb have the structure

9 10

in substantially the cis-(AR,6S) form vherein R and R

9 10

each are 2alkyl or ^ an^ R > taken together with the carbon atom to which they are attached, is cyclohexyl; and 12

R is hydrogen, C^_2alkyl or a metal cation.

In another preferred embodiment of the invention, the compounds of Formula VIII have the structure

VIII

in substantially the cis form vherein R and R each are

9 10

independently hydrogen, Chalky 1 or phenyl; R and R each

9 10

are 2alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclohexyl; and n is zero or 1.

-19-

In another more preferred embodiment of the invention, the compounds of Formula IX have the structure

V

r8 cr^o o

IX

7 8

in substantially the cis-(4R,6S) form wherein R and R each

9 10

are independently hydrogen, C^_^alkyl or phenyl; R and R

9 10

each are C^_^alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclohexyl; and n is zero or 1.

In another aspect, this invention provides novel intermediates of the formula r' ^R10

XI

11 W

1 4

in substantially the cis form wherein R and R each are independently hydrogen, halogen, C^_^alkyl, C^_^alkoxy or

2 3 5 6

trifluoromethy1; R , R , R and R each are independently

-20-

9 10

hydrogen, halogen, C^_^alkyl or C^_^alkoxy; R and R each

9 10

are C^_^alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclopentyl, cyclohexyl

12

or cycloheptyl; and R is hydrogen, ^alkyl or a metal cat ion.

In a preferred embodiment, this invention provides intermediates of Formula XI in substantially the cis form wherein R^, , R^, R^ and R^ each are independently

9 10

hydrogen, fluoro, chloro, methyl or methoxy; R and R each

9 10

are 2alkyl or R and R , taken together with the carbon

12

atom to which they are attached is cyclohexyl and R is hydrogen, C^_^alkyl or a metal cation.

In another preferred embodiment, this invention provides novel intermediates of the formula

-R6 R' ^R10

XII

V—-N

1 4

in substantially the cis - (_4R, 6S ) form wherein R and R each are independently hydrogen, halogen, C^_^alkyl, C^^alkoxy

-21-

BNSDOCID: <GB 2244706A_I_>

2 3 5 6

or trifluoromethy1; R , R , R and R each are independently

9 10

hydrogen, halogen, C^_^alkyl or C^_^alkoxy; R and R each

9 10

12

or cycloheptyl; and R is hydrogen, C^_^alkyl or a tr.etal cation.

*

In another more preferred embodiment, this invention provides intermediates of Formula XII in substantially the cis- (4R,6S) form wherein R^", R^, R^, R^, R^ and R^ each are independently hydrogen, fluoro, chloro, methyl or methoxy;

9 10 9 10

R and R each are C^_2&lky3. or R and R , taken together with the carbon atom to which they are attached, is

12

cyclohexyl and R is hydrogen, C^_^alkyl or a metal cation.

In still another aspect, this invention provides a process for the preparation of an aldehyde of the formula

RVl°

cr^o o

9 10

in substantially the cis form wherein R and R each are 9 10

^alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclopentyl, cyclohexyl or

12

cycloheptyl; and R is hydrogen, C^_^alkyl or a metal cation, comprising the steps of

-22-

reacting a dihydroxy compound of the formula

R8 OH OH 0 VI

7 8

in substantially the erythro form vherein R and R each are independently hydrogen, C^_^alkyl or phenyl which is optionally substituted by one or two C^_^alkyl, halogen, C^_^alkoxy or trif luoromethy 1; R^ is a hydrolyzable ester group; and n is zero or 1, in the presence of a small amount of acid with at least one equivalent of a compound of formula

.R10

cr "H3C

C„,OXoch,

0

9 10

wherein R and R are as defined above, to produce a compound of the formula cr^o

10

1^ .xAAxAv

. . „ VII

-23-

7 8 9 10 11

wherein R , R , R , R , R and n are as defined above;

(b) optionally hydrolyzing an ester of Formula VII to produce a compound of Formula VIII

crS?

] i r fi viii

?10

?B o.

7 8 9 10

wherein R , R , R and R are as defined above; and

(c) oxidizing a compound of Formula VII or a con-pound of Formula VIII to produce a compound of the formula ctSD 0

OR

Ilia

9 10 12

in substantially the cis form vherin R , R and R

are as defined above.

In a preferred embodiment, this invention provides a process for the preparation of an aldehyde of the formula

'I*" irN)

Illb

»ie

-24-

9 in in substantially the cis-(4R,6S) form wherein R and R

9 10

each are C^_^alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclopentyl,

12

cyclohexyl or cycloheptyl; and R is hydrogen, ^alkyl or a metal cation, comprising the steps of

(a) reacting a dihydroxy compound of the formula

1 8

in substantially the erythro form wherein R .and R each are independently hydrogen, C^_^alkyl or phenyl which is optionally substituted by one or two

^alkyl, halogen, C^_^alkoxy or trifluororr,ethyl ; R^ is a hydrolyzable ester group; and n is zero or 1, in the presence of a small amount of acid with at least one equivalent of a compound of formula

9 pio

T

or

CH.

>10

OCH,

-25-

9 10

wherein R and R are as defined above, to produce a compound of the formula

u 0 o

7 8 9 10 11 wherein R , R , R , R , R and n are as defined above;

hydrolyzing an ester of Forr.'jla VII to produce a compound of the formula rV ^10

VIII

rb tr o

V/®5

trS

wherein R^, R®, R^, R^ and n are as defined above:

resolving the acid of Formula VIII to produce a compound of the formula

R'R10

Rs cr^o o IX

-26-

7 8

in substantially the cis-(A R.SS) form vherein R , R , 9 10

R , R and n are as defined above; and

(d) oxidizing the acid of Formula IX and optionally preparing the ester thereof to produce a compound of the formula c ]_Q

in substantially the ci s-(4R,6 S) form wherein R', R 12

and R are as defined above.

In still a further aspect, this invention provides a process for the preparation of a compound of the formula

R5

"< 4

in substantially the trans form vherein R" and R each are independently hydrogen, halogen, C^_^alkyl, C^_^alkoxy, or

-27-

2 3 5

trifluoromethy1; and R , R , R

hydrogen, halogen, C^_^alkyl or steps of

5

and R each are independently

C^_^alkcxy, comprising Ike

(a) reacting a compound of the formula wherein R"*", , R^, R4, R^ ar.d are as defined above and Z is

0 /R14

II 12 Ik J0

— F— (OR ) ? —P — R 7^

\r»

13 14

wherein R is C^_^alkyl; R is phenyl which is unsubstituted or substituted by one of two C^_^alkyl or chloro substituents; and X is bromo, chloro or iodo with a compound of the formula

Ilia

-28-

BNSDOCID: <GB 2244?06A_I_>

9 10

in substantially the cis form wherein R and R each

9 10

are ^alkyl or R and R , taken together with the carbon atcm to which they are attached, is cyclopentyl,

12

cyclohexyl or cycloheptyl; and R is hydrogen,

^alkyl or a metal cation, to produce a compound of the formula

R5

12 3 4

in substantially the cis form vherein R , R , R , R ,

c ti q in to

R , R , R , R and R are as defined above;

reacting a compound of Formula XI with acid to produce a compound of the formula

R5

-29-

wherein r\ R^, R^ , R4, R^, R® and R^ are as defined above; and

12

(c) cyclizing a compound of Formula la wherein R is hydrogen, to produce a compound of formula

12 3 4

in substantially the trans form wherein R , R , R , R , R"* and R^ are as defined above.

In a preferred embodiment, this invention provides a process for the preparation of a conpound of the formula

-30-

BNSDOCID: <GB 2244706A_I_>

in substantially the trans - (4R ^ 6 S) form wherein and 4

R each are independently hydrogen, halogen, C., ^alkyl,

2 3 5 6

C^_^alkoxy, or trifluororr.ethy 1; and R , R , R and R

each are independently hydrogen, halogen, C^_^alkyl or

C^_^alkoxy, comprising the steps of reacting a compound of the formula r!

X

-Z

wherein R1, R2 , R"^ , R4 , R^ and R6 are as defined above and Z is o © 7R14

^13 / 14 .©

— P-(0R13)9 — P — R1

V4

"I -3 14

wherein R is C^^alkyl; R is phenyl which is unsubstituted or substituted by one or two C^_^aikyl or chloro substituents; and X is bromo, chloro or iodo with a compound of the formula

0

R' Dl°

X

Illb 16

-31-

Q

in substantially the cis - f 4R, 6 S ) forrr. wherein R and

R^ cach are C^_^alkyl or R^ and R^, taken together with the carbon atom to which they are attached, is

12

cyclopentyl, cyclohexyl or cycloheptyl; and R is hydrogen, C^_^alkyl or a metal cation, to produce a compound of the formula

1 2

in substantially the cis-(4R,6S) form wherein R , R , R"^, R4, R^, R^, R^, R*^ and R^ are as defined above;

reacting the compound of Forr.ula XII with acid to produce a compound of the formula R5

-32-

wherein r\ R^ , R^, R4, , R^ and R"''2 are as defined above; and

12

(c) cyclizing the compound of Formula lb wherein R is hydrogen, to produce a compound of formula

R5

1 ' 2

in substantially the t rans-(4R , 6 S) form wherein R , R ,

3 A 5 6

R , R , R and R are as defined above.

In Vivo Acute Cholesterol Biosynthesis Inhibition in Rats

Male Wistar rats (160-200 g, housed 2 per cage) were maintained on normal diet (Purina Rat Chow and water, ad libitum) for at least 7 days on a reversed lighting schedule (7:00 a.m. to 5:00 p.m. dark). Food was removed 15 hours prior to dosing. Compounds were administered at 8:00 a.m. by intragastric intubation using 0.5-1.0 mL of water or propylene glycol solutions of sodium salts, lactones, or

-33-

esters of the test compounds. Controls received equal volumes of the vehicle.

Thirty minutes after receiving the test substances,

rats were injected intraperitoneally with 0.9 mL of 0.9^

NaCl containing approximately 120 uCi per kg body weight of sodium [1-^"4C] acetate (1-3 mCi/mrnol) . After a 60 minute incorporation period, rats were sacrificed and liver and blood samples were obtained. Aliquots of plasma (1.0 mL)

obtained by centrifugation of heparin + EDTA-treated blood,

and aliquots of liver horr.cgenates (equivalent to 0.50 g liver wet weight) were taken for determination of radiolabeled 3-hydroxy sterols. Sterol isolation for the liver samples followed the r.ethcd of Kates in Techniques ir.

Lipidology, (M. Kates, ed.) pp. 349, 360-363, North Holland

Publ. Co., Amsterdam, 1972 while the plasma samples were directly saponified followed by isolation of the digitonin-

14

precipitable sterols. C-labelled sterols were quantified by liquid scintillation counting (efficiency corrected).

14

Mean percent inhibition of C incorporated into liver and into plasma cholesterol was calculated for groups of treated animals and compared to mean values for controls conducted simultaneously.

Therefore, the above test provides information oh the ability of test substances to suppress the de r.ovo biosynthesis of cholesterol in vivo in rats with oral

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dosing. For example, using the above test, the compound of Example 13 yielded a 50% Inhibitory Dose (ED^q) for both plasma and liver cholesterol, comparable to values obtained for mevinolin (lovastatin) using a similar procedure [Alberts, e_t ai. , Proc. Nat 1. Acad. Sci . , 77 , 3S57-3961 (1980)].

DESCRIPTION' OF SPECIFIC EMBODIMENTS

In the following examples, all temperatures are given in degrees Centigrade. Melting points were recorded on a

Thomas-Hoover capillary melting point apparatus and are uncorrected. Proton magnetic resonance (^"H NMR) spectra were recorded on a Bruker AM 300, Bruker VM 360 or Varian'

T-60 CW spectrometer, All spectra were determined in CDCl^,

DMSO-d,. or D~0 unless otherwise indicated and chemical D L

shifts are reported in 5 units downfield from the internal standard tetramethylsilane (TMS) and interproton coupling constants are reported in Hertz (Hz). Splitting patterns are designated as follows: s, singlet; d, doublet; t,

triplet; q, quartet; m, multiplet; br, broad peak; dd,.

doublet of doublet; and dq, doublet of quartet. Carbon-13

13

nuclear magnetic resonance ( C NKR) spectra were recorded on a Bruker AM 300 or Bruker VM 360 spectrometer and were broad band proton decoupled. All spectra were determined in CDCl^, DMSO-dg or D^O unless otherwise indicated with

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internal deuterium lock and chemical shifts are reported ia 6 units downfield from tetramethylsilane. Infrared (IR) spectra were determined on a Nicolet KX-1 FT spectrometer from 4000 cm ^ to 400 cm calibrated to 1601 cm absorption of a polystyrene film and are reported in reciprocal centimeters (cm *). Relative intensities are indicated as follows: s (strong), m (medium) and w (weak).

25

Optical rotations [a]p were determined on a Perkin-Elmer 241 polarimeter in CHCl^ at the concentrations indicated.

Analytical thin-layer chromatography (TLC) was carried out on precoated silica gel plates (60F=254) and visualised using UV light, iodine vapors and/or staining with one of the following reagents: (a) methanolic or ethanolic phosphomolybdic acid (2%) and heating; (b) reagent (a) followed by 2% cobalt sulphate in 5M and heating.

Column chromatography, also referred to as flash column chromatography, was performed in a glass column using finely divided silica gel (32-63 jam on silica gel-K) and pressures somewhat above atmospheric pressure with the indicated solvents. Ozonolysis reactions were done using a Velsbach ozonator style 1-23. All evaporations of solvents were performed under reduced pressure. As used herein, the term hexanes is a mixture of isomeric Cg hydrocarbons as specified by the American Chemical Society, and the term "inert" atmosphere is an argon or nitrogen atmosphere unless otherwise indicated.

-36-

Ex amp 1 e 1

Cis -2 , 2 - dime thy 1- 6 - (2- pheny 1 e t hc-r.y 1) - 1, 3 - di ox an a - 4- ace t i c acid methyl ester

Methyl 3 , 5 - dihydroxy-7-phenyl - 6 - en.oat e (98% diastereo-meric purity) (2.37 g, 9.48 rr.mol) was stirred with 2,2-dirr.ethoxypropane (20 rnL) and a catalytic amount of p-toluenesulfonic acid for 16 hours. The solution was partitioned between diethyl ether and dilute aqueous sodium bicarbonate solution. The organic layer was dried (Na^SO^) and concentrated under reduced pressure to afford a yellow solid. After reerystallizat ion from isopropyl ether, 1.70 g (62%) of the title compound was obtained as a white solid; m.p.=84-86.5°C.

Alternatively, 0.2 g of solid sodium carbonate can be added to the 2,2-dimethoxypropane solution and the solution stirred vigorously. The solid is filtered through a fluted filter paper. The excess 2,2-dimethoxypropane is removed under reduced pressure to afford a yellow solid which is recrystallized from isopropyl ether.

1H NMR (CDC13) 6 : 7.37-7.19 (5H, m), 6.59 (IH, d, J = 15.9 Hz), 6.14 (IH, dd, J = 15.9, 6.4 Hz), 4.57-4.35 (IH, m), 4.42-4.35 (IH, m), 3.68 (3H, s), 2.58 (IH, d, J = 15.6, 6.9 Hz), 2.14 (IH, dd, J = 15.6, 6.3 Hz), 1.74-1.61 (lH, m), 1.52 (3H, s), 1.43 (3H, s), 1.45-1.35 (IH, m).

-37-

Anal. Calcd. for ^17^22^4*

Found:

C, 70.32; H, C, 70.24; H,

7.63 7.69.

Example 2

Cis-2,2-dimethyl-6-f 2-phenylethenyl )-l,3-dioxane-4-acetic acid

A solution of 2,2-dimethyl-6-(2-phenylethenyl)-1,3-dioxar.e-4-acetic acid methyl ester (8.5 g, 29.3, r.r.ol) in IN NaOH (32 mL) and methanol (64 nL) was heated to reflux for 45 minutes. After evaporation under reduced pressure, the aqueous solution was washed once with diethyl ether and acidified with IN HCl (33 mL). The precipitate was collected and recrystallized from ethyl acetate/isopropyl ether to afford 7.2 g (90%) of the title compound as a colorless solid; m.p.=153-155°C.

XH NMR (CDCI3) 5 : 7.37-7.20 (5H, m), 6.60 (IH, d, J = 16.0 Hz), 6.14 (IH, dd, J = 16.0, 6.4 Hz), 4.59-4.54 (IH, m)t 4.43-4.35 (IH, m), 2.62 (IH, dd, J = 16.0, 7.2 Hz), 2.51 (IH, dd, J = 16.0, 5.3 Hz), 1.77-1.72 (IH, m), 1.54 (3H, s), 1.46 (3H, s), 1.50-1.36 (IH, m).

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Ana_l. Calcd. for C, 69.54; H, 7.30

Found: C, 69.20; H, 7.33.

Example 3

Resolution cf ci s - 2 , 2-dirr.ethyl- 6 - (2-pheny letheny 1) - 1, 3-dioxane-4-acetic acid

The racenic cis-2,2-dimethyl-6-(2-phenylethenyl)-1,3-

dioxane-4-acetic acid (0.31 g, 1.1 mmol) (prepared in

Example 2) was dissolved in a boiling solution of hexane/

ethanol containing (IS,2R)-ephedrine (0.2 g, 1.1 mmol).

The resulting solution was very slowly brought to room temperature to give 0.21 g (41.4%) of colorless chiral salt

(the usage of diastereomerically pure seed crystal is recommended during the resolution): m.p.=170-171°C.

The chiral acid was freed through an acidic workup

(as described in Example 4) and its enantiomeric purity was determined to be 100% by ^"H NMR using L-phenyltrifluoro-

25

methyl carbinol as a chiral solvent. =+5.45°(c=l,

CHC13).

-39-

Example 4

Cis-(4%,6S)-2 .2-dime thy 1- 6- forinyl- 1, 3-dioxane-4-acetic acid

The resolved salt of cis-2,2-dimethyl-6-(2-phenylethenyl)-1,3-dioxane-4-acetic acid and (IS,2R)-ephedrine (6.6 g, 14.9 mmol) (prepared in Example 3) was partitioned between 0.5N HC1 (30 mL) and diethyl ether. The ether layer was washed with brine, dried (MgS0^/Na2S0^), and concentrated under reduced pressure to afford 4.1 g (99.6%) of the free acid.

This acid was dissolved in dry methylene chloride (100 mL) and ozone was passed through this solution at -78°C until there was deep blue coloration. Excess czcne was removed by purging with nitrogen and the ozonide formed was decomposed by adding CH^SCH^ (5 mL) and warming the solution to room-temperature and allowed to stand for 16 hours. The solution was concentrated under reduced pressure and the residue was dissolved in isoamyl ether (ca 100 mL). The benzaldehyde which was formed during the ozonolysis was azeotroped together with isoamyl ether under reduced pressure to afford the title compound.

XH NMR (CDClj) 5 : 9.57 (IH, s), 4.40-4.30 (2H, m), 2.60 (IH, dd, J = 16.0, 7.0 Hz), 2.49 (IH, dd, J = 16.0, 6.0 Hz), 1.88-1.83 (IH, m) 1.49 (3H, s), 1.46 (3H, s), 1.42-1.31 (IH, m).

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Example 5

Dine thy 1 \ 3,3-bis(A-fluorophenyl)-2-(I-rriethyI-lH-te:razol-5-yl)-2-pi'cper,-l-yl] phosphonate

A slurry of 3,3-bis-(4-fluoropheny1)- 1-bromo-2-(1-ir.ethy 1-IH-t e t razol - 5-y 1) - 2-propane (1.17 g, 3.0 mmol) and trimethyl phosphite (0.41 g, 3.3 mmol) was heated at 100°C for 5 minutes. After cooling to ambient temperature, excess trimethylphosphite was removed jin vacuo to give a light yellow solid. This solid was recrystallized frcm ethyl-acetate/hexane mixture to give the title compound as a pure white solid; m.p. = 140- 141cC.

IR (KBr) v : 1604, 1511 cm"1;

max

XK NMR (CDC13) 6 : 7.7-6.8 (8H, m), 3.6 (3H, s), 3.5 (3H, s), 3.42 (3H, s), 3.2 (2H, d);

Anal. Calcd. for 9^2°3N4P:

C, 54.29; H, 4.56; N, 13.33 Found: C, 53.83; H, 4.48; N, 13.50.

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Example 6

Cis-(4R,6S)-6-r4,A-bisf 4-fluoropheny1) - 3 - (1-methyl- 1H-tetrazol-5-yl)-l, 3-butadienyl 1-2 . 2-dimethyl -1, 3-dioxane-4-acetic acid

The crude chiral acid prepared in Example 4 was dissolved in dry THF (50 mL) and the resulting solution was transferred to a 250 mL three-neck flask purged with nitrogen and equipped with a mechanical stirrer. After the solution was stirred vigorously and cooled to -78cC, n-BuLi (2.5M in hexane, 5.96 mL) was added dropwise. Toward the end of addition, the solution turned into a suspension of white solid-like gel.

A separate flask containing dimethyl [3 ,3-bis(4-fluoro-pheny1)-2-(1-methy1-IH-tetrazol-5-yl)-2-propen-l-yl] phosphonate (6.2 g, 14.7 mmol) (prepared in Example 5) in THF (50 mL) under a nitrogen atmosphere was cooled to -78°C and n-BuLi (2.5M in hexane, 5.96 mL) was added slowly. The resulting red-brown solution was stirred for 15 minutes at -78°C. This solution of phosphonate anion was transferred through a double ended needle to the above vigorously stirred suspension at -78°C containing the lithium salt of the chiral acid. After the addition, the resulting brown solution was stirred for 30 minutes at -78°C and 16 hours at ambient temperature. The THF solution was partitioned

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between 0.5N HCl and ethyl acetate. The organic phase was washed with brine (2x), dried (Na^SO^), and concentrated under reduced pressure. The residue was chromotagraphed on silica gel (66:33:1/diethyl ether:hexane:acetic acid) to afford 3.80 g (51.6% overall yield from the initial ephedrine salt; toluene was employed to azeotrope the residual acetic acid) of the title compound as a yellow foam. [a]D25=+106.1° (c=2.23, CHC13).

*H NMR (CDC13) 5 : 7.24-6.82 (8H, m), 6.62 (IH, d, J = 15.0 Hz), 5.32 (IH, dd, J = 15.0, 5.7 Hz), 4.42-4.37 (IH, m), 4.30-4.23 (IH, m), 3.51 (3H, s), 2.53 (IH, dd, J = 15.9, 7.0 Hz), 2.42 (IH, dd, J = 15.9, 5.6 Hz), 1.62-1.57 (IH, m) , 1.46 (3H, s), 1.33 (3H, s), 1.30-1.20 (IH, m) .

Example 7

Trans -(4R16S)-6-r4,4-bis(4-fluorophenyl)-3-fl-methvl-lH-tetrazol-5-yl)-1.3-butadienyl1 -tetrahydro-4-hydroxy-2H-pyran-2 - one

Cis-(4R,6S)-6-[4,4-bis(4-fluorophenyl) - 3 - (1-methyl-IH tetrazol-5yl)-l,3-but adienyl]-2,2-dimethyl-l,3-dioxane-4-acetic acid (3.7 g, 7.45 mmol) was dissolved in a solution of THF (90 mL) and 0.2N HCl (60 mL) and allowed to stand for 16 hours. The solution was partitioned between ethyl

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BNSDOCID: <GB 2244706A_l_>

acetate and water. The organic layer was washed with brine (2x), dried (Na2S0^), and concentrated under reduced pressure. The residue was dissolved in dry methylene chloride (60 mL) and stirred for 4 hours in the presence of l-cyclohexyl-3-(2-morpholinomethyl) carbcdiimide methc-p-toluenesulfonate (6.6 g, 15.6 mmol). The solution was concentrated under reduced pressure and the residue was partioned between ethyl acetate and water. The organic layer was dried (N'a^SO^) and concentrated under reduced pressure. The residue was purified by chromatography on silica gel (l:l/ethyl acetate:diethyl ether). After recrystallization from ethyl acetate-hexane, 1.33 g (40.1%) of the title compound was obtained as a white solid; m.p.= 172-173°C. [a]D25 = + 237.S° (c = 2.17, CHCl-j).

Example 8

Methyl 3-hvdroxy-5-oxo-6,8-decadienoate

To a cold (-30°C) solution of methyl acetoacetate (41.5 g, 357 mmol) in THF (500 mL) was added lithium diisopropylamide (476 mL, 1.5M solution in cyclohexane, 714 mmol). The resultant solution was stirred for 15 minutes at -30°C. After cooling to -78°C, 2,4-hexadienal (34.3 g, 357 mmol) was added and the solution stirred for 10 minutes at -78°C and for 16 hours at ambient temperature. The solution

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was concentrated under reduced pressure and the residual syrup was partitioned between IN HCl and ethyl acetate. The organic layer was washed with brine (2x), dried (Na^SO^), and concentrated. The residue was purified by chromatography on silica gel (diethyl ether:hexane/2:1) to afford 18.5 g (24.4%) of the title compound as an oil.

1H NMR for (E) (E) isomer (200MHz, CDC13) 5 : 6.3 (IH, dd, J = 14.7, 11.9Hz), 6.02 (IH, dd, J = 14.7, 11.9Hz), 5.75 (IH, dq, J = 14.7, 6.4Hz), 5.5 (IH, dd, J = 18.7, 6.4Hz), 4.74-4.5 (IH, m), 3.73 (3H, s), 3.51 (2H, s), 2.6 (2H, d, J = 5.8Hz), 1.77 (3H, d, J = 6.4Hz).

Example 9

Methyl 3,5-dihydroxy-6,8-decadienoate

To a cold (-15°C) solution of methyl 3-hydroxy-5-oxo-6,8-decadienoate (18.5 g, 86.9 mmol) in THF (300 mL) was added triethylborane (1M in THF, 113 mL, 113 mmol) and the solution was stirred for 20 minutes. After the mixture was cooled to -78°C, NaBH^ (6 g, 159 mmol) and methanol (37.5 mL) were added. The solution was vigorously stirred for 30 minutes at -78°C and at ambient temperature for 3 hours. The solvent was removed under reduced pressure and the residue was partitioned between IN HCl and ethyl acetate.

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The organic layer was dried (Na2S0^) and concentrated. The residue was purified by chromatography cn silica gel (diethyl ether:hexane/3:1) to afford 7.95 g (42.7%) of the title compound as a yellow oil.

KMR for (E) (E) isomer (360MHz, CDC13) 6 : 6.18 (IH, dd, J = 15.1, 10.4Hz), 6.00 (IH, dd, J = 15.1, 10.4Hz), 5.6 9 (IH, dq, J = 15.1, 7.0Hz), 5.52 (IH, dd, J = 15.1, 6.7Hz), 4.46-4.37 (IH, m), 4.29-4.22 (IH, m), 3.69 (3H, s), 2.60-2.42 (2H, m), 1.72 (3H, d, J = 7.0Hz), 1.74-1.57 (2H, m) .

Exar.pl e 10

i

Methyl cis-4 - (1, 3-pentadienvl) -1.5-dioxaspiror5. 51ur.de cane -2-acetate

Methyl 3,5-dihydroxy-6,8-decadienoate (7.6 g, 35.5 mmol) and p-toluenesulfonic acid (0.1 g) was added to cyclohexanone (10 g, 100 mmol) and stirred for 16 hours at ambient temperature. The yellow solution was loaded directly onto a silica gel column and the product eluted with diethyl etherrhexane (1:4). The appropriate fractions were combined to give 3.52 g (33.6%) of the title compound as a colorless oil.

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BNSDOCID: <GB_ 2244706A l_>

1H KMR for (E) (E) isomer (3 60MHz), CDC13) 5 : 6.16 (IH, dd, J = 15.1, 10.6Hz), 6.00 (IH, dd, J = 15.1, 10.6Hz), 5.71-5.65 (IH, dd, J = 15.1, 6.5Hz), 5.47 (IH, dd, J = 15.1, 6.4Hz), 4.44-4.39 (IH, m), 4.35-4.30 (IH, m), 3.66 (3H, s), 2.52 (IH, dd, J = 1.54, 7.9Hz), 2.30 (IH, dd, J = 15.4, 6,5Hz), 2.1-1.18 (12H, m), 1.72 (3H, d, J = 6.5Hz).

Anal. Calcd. for (-'i7^26^4: ^' ^9.36; 8.90

Found: C, 69.59; H, 9.16.

Example 11

Cis-4-(l,3-per.tadienyl)-l,5-dioxas?iror5.5lundecar.e-2-acetic acid ;

Methyl 4-(l,3-pentadieny-l)-l,5-dioxaspiro[5. 5 jundec ane -2-acetate (3.5 g, 12.4 mmol) was heated to reflux in a solution of IN NaOH (13 mL) and methanol (26 mL). Methanol was removed under reduced pressure and the remaining aqueous solution was acidified with IN HCl and extracted with diethyl ether. The organic layer was dried (Na2S0^) and concentrated. The residual solid was recrystallized from ethyl acetate/hexane to afford 2.0 g (55.9%) of the title compound as a colorless solid; m.p.=144-146.5°C.

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1H NMR (36CMHz, CDCl-j) 6 : 6.18 (IH, dd, J = 18.0, 12.5Hz), 5.72 (IH, dq, J = 18.0, 7.7Hz), 5.99 (IH, dd, J = 18.0, 12.5Hz), 5.48 (IH, dd, J = 18.0, 7.6Hz), 4.45-4.37 (IH, m), 4.37-4.25 (IH, m), 2.56 (IH, dd, J = IB.9, 8.8Hz), 2.48 (IH, dd, J = 18.9, 6.1Hz), 2.60-1.30 (12H, m), 1.73 (3H, d, J = 7.7Hz).

Anal. Calcd. for <-']_g^24^4: ^S .54; H, 8.62

Found: C, 68.36; H, 8.55.

Example 12

Cis-4-r4,4-bis(4 - fluoropheny1)- 3 -(1-nethvl-IH-tetrazol-5-yl)-1,3-butadienyll- 1. 5-dioxaspirof5.5lundecane-2 - acetic acid

A. 4-Formyl- l,5-dioxaspiror5.5]ur.decane-2-acetic acid

Ozone was passed through a solution of 4-(1,3-penta-dienyl)-!,5-dioxaspiro[5.5]undecane-2-acetic acid (570 mg, 2.0 mmol) in methylene chloride (25 mL) at -78°C. After the solution had attained a blue color, nitrogen was passed through the solution to remove the excr-ss ozone. Dimethyl, sulfide (0.5 mL) was added and the solution was concentrated under reduced pressure to afford the title compound as a viscous oil which was used without further purification in the subsequent step.

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BNSDOCID: <GB ' 224470AA |_>

NMR (601-iHz, CDC13) 6 : 9.5 7 (IH, s), 4.52-4.14 (2K, m), 2.60-2.31 (2H, m), 2.10-1.10 (12H, m).

B. Cis- 4 -f4.4 -bi s(4 -flu oropheny1)- 3 -(1-methy1-1H-tetrazol-5-yl)-l,3-butadicnyl]-l,5-dioxaspiro-f 5 . 5 1ur.de cane - 2 - acid

To a solution of dimethyl [3 , 3-bis(4-fluoropheny1)-2-(1 -methyl-lH-tetrazol-5-vl)-2-propenyl] phosphonate (1.7 g, 4 mmol) in THF (20 mL) at -78eC was added n-BuLi (1.6 mL, 4 mmol, 2.5M in hexane). The resultant brown-red solution was stirred for 30 minutes at -78°C. Using a double ended needle, this solution was transferred to a solution cont aining 4-formy1- l,5-dioxaspiro[5.5]undecane-2-acetic acid (prepared in Step A) in TKF (10 mL) and maintained at -78°C. After the transfer had been completed, the combined reaction mixture was stirred at -78°C for 1 hour and at ambient temperature for 4 hours. The solution was then partitioned between 0.5N HCl and ethyl acetate. The organic layer was washed with brine (2x), dried (Na^SO^), and concentrated under reduced pressure. The residue was purified by chromatography on silica gel (diethyl ether:hexane:acetic acid/50:20 :1) to afford 342 mg (31.9% overall yield) of the title compound as a yellow foam.

-49-

1H NMR (360MHz, CDCl3) b : 7.25-6.84 (8H, m), 6.66 (IH, d, J = 16.0Hz), 5.32 (IH, dd, J = 16.0, 5.10Hz), 4.45-4.25 (2H, m), 3.52 (3H, s), 2.56 (IH, dd, J = 16.0, 7.6Hz), 2.44 (IH, dd, J = 16.0, 5.1Hz), 1.89-1.17 (12H, m).

Example 13

Trans-6~r4,4-bis(4- £luoropheny1)-3-(l-methy l-lH-tet;.a2ol-5-vl)-1.3-butadieny11-tetrahvdro-4-hydroxy-2H-pyran-2-one

A mixture of 4-[4,4-bis(4-fluoropheny1)-3-(1-methy1-1H-tetrazol-5-yl)-l,3-butadienyl]-1,5-dioxaspiro[5.5]undecane-2-acetic acid (280 mg, 0.52 mmol) in 20 mL of THF/0.5N HCl (1:1) was allowed to stand at ambient temperature for 26 hours. The solution was partitioned between brine and ethyl acetate. The organic layer was washed with brine (2x), dried (Na^SO^) and concentrated. The resultant foam (126 mg) was dissolved in dry methylene chloride (10 mL) and treated with l-cyclohexyl-3-(2-morpholinomethyl) carbodiimide metho-p-toluenesulfonate (0.24 g). After 16 hours at ambient temperature, the solution was evaporated under reduced pressure and the residue was purified by silica gel chromatography using ethyl acetate as eluent. The appropriate fractions afforded 38 mg (16.670) of the title compound as a colorless oil which is a racemic mixture of the compound of Example 7.

-50-

Example 14

Methyl 2 , 2- dimethy 1 - 6- fomy 1 - 1, 3 -d i oxane-4-acetate

Cis- 2,2-dimethyl- 6 -(2-phenyletheny1)- 1,3-dioxane-4-acetic acid methyl ester (prepared in Example 1) was dissolved ia methanol (10 mL) and ozone was passed through the solution at -78°C until the color of the solution turned blue. The reaction mixture was purged with nitrogen to remove excess ozone then dimethyl sulfide was added and the temperature was allowed to warm up to room temperature. The reaction was evaporated i_n vacuo and the residual oil was purified by chromatography on silica gel using diethyl ether-hexane (3:1) as the eluent to afford the title compound.

NMR (360MHz, CDC13) 6 : 9.53 (IH, s), 4.40-4.23 (2H, m), 3.69 (3H, s), 2.53 (IH, dd, J = 15.8, 7.02 Hz), 2.37 (IH, dd, J = 15,8, 5.98 Hz), 1.85-1.76 (IH, m), 1.44 (3H, s), 1.40 (3H, s), 1.35-1.23 (IH, m).

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Example 15

3 , 3-5is ( 4-f luoropheny 1) -l-brcmo-2- (l-r.ethyl-lH-tetrazo3 -5-Yllzl -propene

A. 5-Ethyl-l-methyl-lH-tetrazole

To a slurry of 1,5-dimethyltetrazcle (4.9 g, 0.05 mole) in dry tetrahydrofuran (50 mL) was added 2.5M n-butyllithium in hexanes (20 mL, 0.05 Tr.ole) over a period of 15 minutes at -78°C under an inert atmosphere. This mixture was stirred for 3 0 minutes and a yellowish precipitate formed during this time. Kethyl iodide (3.7 mL, 0.06 mole) was then added over a period of 15 minutes.

After stirring for an additional 30 minutes, the clear reaction mixture was diluted with water and extracted with ethyl acetate (3 x 50 mL). The aqueous layer was washed with chloroform (2 x 25 mL), and the combined organic layer was dried over sodium sulfate and concentrated under reduced pressure to give an oil. The oil was purified by distillation to give 5.2 g (92%) of the title compound; b.p. = 89-90°C at 0.05 mm Hg.

NMR (CDC13) 6 : 4.05 (s, 3H), 2.86 (q, 2H),

1.41 (t, 3H);

13C NMR (CDC13) 6 : 156.0, 33.24, 16.75, 11.20.

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B . 1, l-Bis(4-fluorophenyl)-2- (1-methyl-lH-tetrazol-5-yl~)propanol

To a solution of 5-ethy1-1-methyl-IH-tetrazole (5.6 g, 0.05 mole) [prepared in Step A] in 60 mL of dry tetrahydrofuran was added 2. 5M n-butyllithiurn (20 mL, 0.05 mole) in hexane over 5 minutes at -78°C (bath temperature) under an inert atmosphere. The mixture was stirred for 30 minutes and a solution of 4,4'-dif1uorobensophenone (10.8 g, 0.5 mole) in 25 inL of dry tetrahydrofuran was added over 5 minutes. This mixture was stirred for an additional 2 hours while the bath temperature was slowly warmed to -20°C. The reaction was quenched with IN* HCl and extracted with ethyl acetate (3 x 50 mL) and chloroform (3 x 50 mL). The combined organic layer was dried over sodium sulfate and : concentrated under reduced pressure to give a white solid. The solid was purified by crystallization from ethanol-hexane to give 10.8 g (65%) of the title compound; m.p. = 160-161°C.

IR (KBr) v : 3400 cm"1;

v ' max

1H NMR (CDC13) 6 : 7.8-7.02 (m, 8H), 5.95 (s, IH), 4.65 (q, IH), 3.98 (s, 3H), 1.29 (d, 2H).

-53-

13c KMR (CDC13) 5 : 162.57, 162.37, 159.14, 156.71, 142.48, 140.54, 128.25, 128.13, 127.52, 127.42, 114.67, 114.41, 114.38, 78.56, 36.99, 33.43, 14.52.

Anal. Calcd. for

C, 61.81; H, 4.88; N, 16.96 Found: C, 61.79; H, 4.90; N, 17.09.

C. l.l-Bis(4- finorophenyl)- 2-(1-methyl-lH-tetrare1-5-yl)-l-prcpene

A slurry of l,l-bis(4-fluorophenyl)-2-(l-methyl-lH-tetrazol-5-yl)propanol (8.25 g, 0.025 mole) [prepared in Step B] and 100 mg of p-toluene sulfonic acid monohydrate in xylene (60 mL) was heated to reflux with a' Dean & Stark water collecting apparatus for a period of 12 hours. The reaction mixture was washed with IN NaCH (10 mL) while it was warm and with water (100 mL). Concentration of the organic layer gave off-white crystals of product. This was purified by recrystallization from ethanol-hexane to give 7,1 g (91%) of the title compound as white crystals; m.p. = 146-147°C.

IR (KBr) v ; 1575; 1500 cm"1.

ITiflA

XH NMR (CDC13) 6 : 7.42-6.85 (m, 8H), 3.53 (s, 3H), 2.14 (s, 3H);

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13C NMR (CDC13) 6 : 163.37, 163.08, 160.13, 155.61, 144.60, 145.34, 136.47, 136.42, 136.24, 136.19, 131.65, 131.54, 131.11, 131.01, 119.53, 115.51, 115.27, 115.22, 33.50, 21.20.

Anal. Calcd. for C,-,H., , F,*N, :

17 14 2 4

C, 65.37; H, 4.51; N, 17.94 Found: C, 65.64; H, 4.61; N, 18.09.

D . 3,3-Bis(4- f lu or o phenyl )-l-bromo-2- fl-methyl- 1H-tetrazol-5-yl)-2-propene

A slurry of 1,1-bis(4-fluoropheny1)-2-( 1-methy1-IH-1etrazol-5-y1)- 1-propene (61.46 g, 0.197 mole) [prepared in Step C] , N-bromosuccinimide (35.06 g, "0.197 . mole) and catalytic amount of azobis isobutyronitrile or benzoyl peroxide in carbon tetrachloride (1.2 liters) was heated to reflux in an inert atmosphere for a period of 2 hours. The reaction mixture was cooled to ambient temperature and the solid from the reaction was filtered. The filtrate was concentrated under reduced pressure and the solid obtained was recrystallized from toluene-hexane to give 72 g (93%) of the title compound as white crystals; m.p. = 159-160°C.

IR (KBr) v : 1600 cm"1.

v ' max

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*H NMR (CDC13) 5 : 7.5-7.1 (m, 8H), 4.44 (s, 2H), 3.53 (s, 3H).

13C NMR (CDC13) 6 : 163.94, 163.74, 160.60, 160.45, 143.42, 149.68, 135.20, 135.15, 134.69, 131.43, 131.31, 130.90, 130.80, 119.57, 115.94, 115.77, 115.65, 115.50.

Anal. Calcd. for C, -H, -,F„BrN, : 1/ 13 2 4

C, 52.19; H, 3.34; N, 14.32 Found: C, 52.58; H, 3.47; N, 14.49.

Example 16

rig-Bis (4 - fluoropheny 1) -2 - ( 1-methyl-IH-tetrazol-5-yl)-

1-propen-3-yl1triphenylphosphonium bromide

A slurry of 3 , 3-bis (4-f luorcphenyl) - l-brcmo-

2-(1-methyl-IH-tetrazol-5-yl)-2-propene (1.95 g, 0.005 mole) [prepared in Example 15, Step D] and triphenylphosphine (1.3 g, 0.005 mole) in cyclohexane (25 mL) was heated to reflux. The reaction mixture became a clear solution after 30 minutes and a white precipitate appeared after 1 hour. The mixture was heated for an additional 8 hours, cooled to ambient temperature and the solid was collected by filtration and washed with diethyl ether. This white powder was dried in vacuum at 50°C to give 3.0 g (92%) of the title compound; m.p. = 254-255°C.

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IR (KBr) v : 3450, 1600, 1500, 1425 cm"1. ^ ' max

1H NHR (DMSO-d6) 6 : 7.92-6.80 (m, 23H), 4.94 (6d, 2H), 3.83 (s, 3K);

13C NMR (DM S 0-d 6 ) 6 : 163.53 , 163.36, 160.28 ,

160.87, 154.04, 153.89, 152.76, 135.11, 134.79, 134.16,

133.68, 133.54, 130.53, 130.45, 130.35, 130.21, 130.07,

118.02, 116.89, 116.18, 115.89, 115.62, 115.32, 111.43, 111.39, 34.22, 28.88, 28.22.

Anal. Calcd. for C-^H2gBrF2N^P:

C, 64.31; H, 4.32; N, 8.57 Found: C, 64.02; H, 4.37; N, 8.89..

BNSDOCID: <GB 2244706A_I_>

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Claims

WHAT IS CLAIMED IS;

1. A compound of the formula in substantially the cis form wherein

1 4

R and R

R2, R3, R5 and R^

R9 and R10

,12

each are independently hydrogen, halogen, C^_^ alkyl, alkoxy or trifluorcnsthyl;

each are independently hydrogen, halogen,

C^_^ alkyl or alkoxy;

9 10

each are C^ ^ alkyl or R and R , taken together with the carbon atom to which they are attached, is cyclopentyl, cyclohexyl or cycloheptyl; and is hydrogen, C-^-4 alkyl or a metal cation.

2. A compound of claim 1 having the formula

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wherein

R^ and each are alkyl or R^ and R , taken together with the carbon stem to which they are attached, is cyclopentyl, cyclohexyl or cycloheptyl; and is hydrogen, alkyl or a metal cation.

9 10

3. A compound cf claim 2 wherein R and R each are 12

methyl; and R is hydrogen, alkyl or a metal cation.

12

4. The compound of claim 3 wherein R ^ is hydrogen.

9 10

5. A compound of claim 2 wherein R and R~ , taken together with the carbon atom to which they are attached, is

12

cyclohexyl; s.r.d R is hydrogen, ^ alkyl or a metal caticn

12

6. The comocur.d cf claim :> wherein ?v is rv-'drcgen.

7. A compound cf claim 1 having the formula

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in substantially the cis-(4P>,65) form wherein

R1 and R^ esch are independently hydrogen, halogen,

R2, R3, R5

alkyl, C]__4 alkoxy or triflucroir.ethy 1

and R^ each are independently hydrogen, halogen,

alkyl, or C^_^_ alkoxy;

R9 and Ri0 each are C]__4 alkyl or R9 and R10, taken together with the cr.rbon atcr, to which they are attached, is cyclopentyl, cyclohe;-:yl or cyclche-tyl; and R12 is hydrogen, alkyl or a r-.etal cation.

wherein

R9 and R10 each are alkyl or R9 and R10, taken together with the carbon atom to which they are attached, is cyclopentyl, cyclohexyl or cycloheptyl; and R12 is hydrogen, alkyl or a metal cation.

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9. A compound of claim 8 12

methyl; and R is hydrogen,

10. The compound of claim

11. A compound cf claim 8

together with the carbon atom to

12

cyclohexyl; and R - is hydrogen,

12. The compound of claim

9 10

wherein R and R each are

4 alkyl or a metal cation.

12

9 wherein R is hydrogen.

, . _ 9 , 10 , wherein R and R , tcKen which they are attached, is alkyl or a metal cation.

12. , .

11 vnerem R is hycrcgen.

13. A compound according to any one of ciairr.s 1 tc 12 substantially as hereinbefore describe::.

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Published 1991 at The Patent Office. Concept House, Cardiff Road. Newport. Gwent NP9 1RH. Further copies may be obtained from BNSDOCID <GB .Branch. Unit 6, Nine Mile Point. Cwmfellnfach. Cross Keys, Newport. NPl 7HZ. Prtated by Multiplex techniques ltd, St Maiy Cray. Kent.