[go: up one dir, main page]

CU23095A1 - Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado - Google Patents

Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Info

Publication number
CU23095A1
CU23095A1 CU20000247A CU20000247A CU23095A1 CU 23095 A1 CU23095 A1 CU 23095A1 CU 20000247 A CU20000247 A CU 20000247A CU 20000247 A CU20000247 A CU 20000247A CU 23095 A1 CU23095 A1 CU 23095A1
Authority
CU
Cuba
Prior art keywords
typification
microorganisms
reagent
quick
molecules
Prior art date
Application number
CU20000247A
Other languages
English (en)
Inventor
Diaz Oscar Arencibia
Canovas Lilia Lopez
Donderiz Hilda Teresa Clark
Orozco Esther Orozco
Acuna Maria Concepcion Ariosa
Alonso Axel Sanchez
Perez Regnar Gigato
Clarke David Higginson
Rojas Ana Maria Riveron
Original Assignee
Cnic Ct Nac Investigaciones
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cnic Ct Nac Investigaciones filed Critical Cnic Ct Nac Investigaciones
Priority to CU20000247A priority Critical patent/CU23095A1/es
Priority to DK01983428T priority patent/DK1350852T3/da
Priority to AU2002214939A priority patent/AU2002214939A1/en
Priority to DE60125832T priority patent/DE60125832T2/de
Priority to PT01983428T priority patent/PT1350852E/pt
Priority to AT01983428T priority patent/ATE350490T1/de
Priority to MXPA03004006A priority patent/MXPA03004006A/es
Priority to EP01983428A priority patent/EP1350852B1/en
Priority to JP2002542115A priority patent/JP3955817B2/ja
Priority to CNB018203035A priority patent/CN100532569C/zh
Priority to BRPI0115172-0A priority patent/BR0115172B1/pt
Priority to CA2428313A priority patent/CA2428313C/en
Priority to US10/415,950 priority patent/US7731828B2/en
Priority to ES01983428T priority patent/ES2276843T3/es
Priority to PCT/CU2001/000008 priority patent/WO2002038802A1/es
Priority to ZA200303472A priority patent/ZA200303472B/en
Priority to EC2003004577A priority patent/ECSP034577A/es
Priority to HK04106583.6A priority patent/HK1063822A1/xx
Publication of CU23095A1 publication Critical patent/CU23095A1/es
Priority to CY20071100340T priority patent/CY1106369T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Se provee un proceso para tipificar rápidamente levaduras, parásitos y bacterias. Comprende los siguientes pasos: a) Preparar, en 5 a 60 minutos, ADN intacto e inmovilizado mediante un método que emplea un juego de reactivos que solo contiene solución tampón, un detergente, un agente quelante y un agente que rompe los puentes de hidrógeno. b) Separar moléculas de ADN intactas o sus fragmentos de restricción usando miniequipos de electrofóresis de campos pulsantes de los sistemas CHEF (Contour Clamped Homogeneous Electric Field) y TAFE (Transversal Alternating Field Electrophoresis) en tiempos comprendidos entre 2.5 y 7 horas. c) Seleccionar las condiciones óptimas que se aplicarán en el miniCHEF empleando un método que simula a priori los patrones electroforéticos que se obtendrían en dichos geles. d) Brindar los tiempos de reorientación, velocidades de migración y tallas de las moléculas sin emplear marcadores de tallas pero valiéndose de un método que analiza distancias migradas.
CU20000247A 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado CU23095A1 (es)

Priority Applications (19)

Application Number Priority Date Filing Date Title
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
CNB018203035A CN100532569C (zh) 2000-11-07 2001-11-02 微生物快速分型方法及所用试剂盒
BRPI0115172-0A BR0115172B1 (pt) 2000-11-07 2001-11-02 Processo para a tipificação bacteriana rápida por meio de eletroforese de campo pulsado em gel
DE60125832T DE60125832T2 (de) 2000-11-07 2001-11-02 Verfahren zur schnellen Typisierung von Mikroorganismen durch Puls-Feld-Gelektrophorese, wobei die Proben in einer autoklavierbaren und flexiblen Form hergestellt werden
PT01983428T PT1350852E (pt) 2000-11-07 2001-11-02 Método para tipagem rápida de microrganismos e conjunto de reagentes utilizados
AT01983428T ATE350490T1 (de) 2000-11-07 2001-11-02 Verfahren zur schnellen typisierung von mikroorganismen durch puls-feld-gelektrophorese, wobei die proben in einer autoklavierbaren und flexiblen form hergestellt werden
MXPA03004006A MXPA03004006A (es) 2000-11-07 2001-11-02 Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado.
EP01983428A EP1350852B1 (en) 2000-11-07 2001-11-02 Method for rapid typification of microorganisms by puls field gel electrophoresis whereby the samples are prepared in a autoclavable and flexible mould
JP2002542115A JP3955817B2 (ja) 2000-11-07 2001-11-02 迅速微生物型分類法及び関連試薬キット
DK01983428T DK1350852T3 (da) 2000-11-07 2001-11-02 Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form
AU2002214939A AU2002214939A1 (en) 2000-11-07 2001-11-02 Method for rapid typification of microorganisms and set of reagents used
CA2428313A CA2428313C (en) 2000-11-07 2001-11-02 Method for rapid typification of microorganisms by pulse field gel electrophoresis (pfge) whereby the samples are prepared in an autoclave and flexible mould
US10/415,950 US7731828B2 (en) 2000-11-07 2001-11-02 Method for rapid typification of microorganisms and set of reagents used
ES01983428T ES2276843T3 (es) 2000-11-07 2001-11-02 Metodo para tipificar rapidamente microorganismos mediante electroforesis en gel de campo pulsado en el que las muestras se preparan en un molde flexible que se puede esterilizar en autoclave.
PCT/CU2001/000008 WO2002038802A1 (es) 2000-11-07 2001-11-02 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
ZA200303472A ZA200303472B (en) 2000-11-07 2003-05-06 Process for rapid microorganism typing and associated kit of regents.
EC2003004577A ECSP034577A (es) 2000-11-07 2003-05-07 Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado
HK04106583.6A HK1063822A1 (en) 2000-11-07 2004-09-01 Method for rapid typification of microorganisms and set of reagents used
CY20071100340T CY1106369T1 (el) 2000-11-07 2007-03-12 Μεθοδος ταχειας τυποποιησης μικροοργανισμων με ηλεκτροφορηση πηκτης παλλομενου πεδιου με την οποια τα δειγματα παρασκευαζονται σε ενα εγκλειομενο σε αυτοκλειστο και ευκαμπτο καλουπι

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Publications (1)

Publication Number Publication Date
CU23095A1 true CU23095A1 (es) 2005-11-18

Family

ID=40134841

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Country Status (19)

Country Link
US (1) US7731828B2 (es)
EP (1) EP1350852B1 (es)
JP (1) JP3955817B2 (es)
CN (1) CN100532569C (es)
AT (1) ATE350490T1 (es)
AU (1) AU2002214939A1 (es)
BR (1) BR0115172B1 (es)
CA (1) CA2428313C (es)
CU (1) CU23095A1 (es)
CY (1) CY1106369T1 (es)
DE (1) DE60125832T2 (es)
DK (1) DK1350852T3 (es)
EC (1) ECSP034577A (es)
ES (1) ES2276843T3 (es)
HK (1) HK1063822A1 (es)
MX (1) MXPA03004006A (es)
PT (1) PT1350852E (es)
WO (1) WO2002038802A1 (es)
ZA (1) ZA200303472B (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
CA2418582A1 (en) * 2003-02-07 2004-08-07 Dupont Canada Inc. A method for simulating and modeling the presence and growth of microbes, including pathogens and spoilage organisms through a food supply chain
US20080079726A1 (en) * 2006-07-03 2008-04-03 Wolfgang Geiger Visual display of process sequences
US20080044822A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US20090286694A1 (en) * 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20100056388A1 (en) * 2006-08-21 2010-03-04 Cnvgenes, Inc. Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
WO2012170560A2 (en) 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
AU2012273121B2 (en) 2011-06-19 2016-08-25 Abogen, Inc. Devices, solutions and methods for sample collection
CN103196980B (zh) * 2013-03-27 2014-10-08 中国水产科学研究院淡水渔业研究中心 鱼源链球菌脉冲场凝胶电泳快速分型方法
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
US9999856B2 (en) 2013-07-26 2018-06-19 General Electric Company Methods for electroelution of biomolecules
SG10201807736SA (en) * 2014-03-07 2018-10-30 Dna Genotek Inc Composition and method for stabilizing nucleic acids in biological samples
CN105372318B (zh) * 2014-08-28 2018-01-05 山东省肿瘤医院 将细胞原位电泳与流式分析术结合的细胞固定方法
CN104561326B (zh) * 2015-01-16 2017-02-22 天津医科大学 一种基于菌毛多样性的尿路感染大肠杆菌的分型方法
US11383240B2 (en) 2016-05-22 2022-07-12 Cornell University Single cell whole genome amplification via micropillar arrays under flow conditions
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
KR102388753B1 (ko) * 2020-07-20 2022-04-20 광주과학기술원 유전영동 힘을 이용한 미생물 검출 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707237A (en) * 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US4861448A (en) 1982-11-18 1989-08-29 The Trustees Of Columbia University In The City Of New York Electrophoretic methods employing gel inserts
US4473452A (en) 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
CA2052037A1 (en) * 1990-10-02 1992-04-03 Eric Lai Automatic control system for improved electrophoresis
FR2693209B1 (fr) * 1992-07-03 1994-09-30 Inst Nat Sante Rech Med Procédé de détermination de la taille en nucléotides de fragments d'ADN.
JP3866762B2 (ja) 1993-11-29 2007-01-10 ジェン−プローブ・インコーポレイテッド 広範な生物からの核酸抽出法
ATE294386T1 (de) * 1998-02-16 2005-05-15 Mallinckrodt Inc Behälter für ein elektroforetisches gel und zugehöriges verwendungsverfahren

Also Published As

Publication number Publication date
CA2428313A1 (en) 2002-05-16
CN100532569C (zh) 2009-08-26
US20040050700A1 (en) 2004-03-18
US7731828B2 (en) 2010-06-08
JP2004513375A (ja) 2004-04-30
DE60125832T2 (de) 2007-10-11
HK1063822A1 (en) 2005-01-14
CY1106369T1 (el) 2011-10-12
ES2276843T3 (es) 2007-07-01
BR0115172B1 (pt) 2014-10-21
CA2428313C (en) 2010-09-14
AU2002214939A1 (en) 2002-05-21
MXPA03004006A (es) 2004-04-02
EP1350852A1 (en) 2003-10-08
ZA200303472B (en) 2003-11-11
ECSP034577A (es) 2003-08-29
BR0115172A (pt) 2004-02-17
JP3955817B2 (ja) 2007-08-08
WO2002038802A1 (es) 2002-05-16
PT1350852E (pt) 2007-03-30
CN1479790A (zh) 2004-03-03
DK1350852T3 (da) 2007-05-07
EP1350852B1 (en) 2007-01-03
ATE350490T1 (de) 2007-01-15
DE60125832D1 (de) 2007-02-15

Similar Documents

Publication Publication Date Title
CU23095A1 (es) Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
ATE403740T1 (de) Verfahren, werkzeuge und reagenzien für zelltrennung
BR9911023A (pt) Método para separar magneticamente partìculas selecionadas, particularmente células biológicas e aparelho para separar magneticamente partìculas selecionadas
DK1055112T3 (da) Fremgangsmåde til en blanding
ATE151433T1 (de) Verfahren und kit zur reinigung von nukleinsäure
BR0200585A (pt) Reagente e processo para a identificação e contagem de células biológicas em uma amostra
DE69939945D1 (de) Verfahren zur isolierung von stammzellen
ATE40478T1 (de) Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit.
EP0871645A4 (en) FAS / AP01 RECEPTOR FUNCTION MODULATORS
ES2078007T3 (es) Procedimiento y medio de identificacion de bacterias del genero listeria.
DE69533144D1 (de) Methode zur zellyse
ES2144604T3 (es) Inmunosubstraccion electroforetica capilar para clasificar y determinar las proteinas m.
BRPI0415884A (pt) imobilização de biocatalizador
ATE397658T1 (de) Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren
DE3767209D1 (de) Reagenz zum erreichen einer transparenz in biologischen fluessigkeiten und seine analytischen anwendungen.
ES8800757A1 (es) Procedimiento para preparar un reactivo para valorar por hemaglutinacion anticuerpos dirigidos contra toxinas bacterianas
ES2193349T3 (es) Metodo para la produccion de rdspa alfa1.
Danielsen et al. Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies
DE602005026412D1 (de) Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten
TR199800702A2 (xx) Boya maddeleri içeren atık suların biyolojik işlemleri.
ZA866956B (en) Methods for utilizing cell lines which lack human class ii histocompatibility antigens
ATE295222T1 (de) Verfahren und vorrichtung zur elektrophoretischen trennung von partikeln, insbesondere von makromolekülen
SU833518A1 (ru) Способ химического обогащени флотационногофлюОРиТОВОгО КОНцЕНТРАТА
UA10528A (uk) Спосіб впливу hа призабійhу зоhу пласта