CS216388B1 - Method of determination of the calcium concentration - Google Patents
Method of determination of the calcium concentration Download PDFInfo
- Publication number
- CS216388B1 CS216388B1 CS585780A CS585780A CS216388B1 CS 216388 B1 CS216388 B1 CS 216388B1 CS 585780 A CS585780 A CS 585780A CS 585780 A CS585780 A CS 585780A CS 216388 B1 CS216388 B1 CS 216388B1
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- calcium
- determination
- chromogen
- biological material
- working solution
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Vynález se týká způsobu stanovení vápníku, především v biologickém materiálu a ve vodě. Podstata způsobu stanovení vápníku podle vynálezu spočívá v tom, že se ke zkoumanému vzorku přidá 1-5 ml pracovního roztoku, tvořeného stejnámi díly chromogenu monoetanolaminového pufru. Chromogen obsahuje kresolftalexon a 8-hydroxichinolin rozpuštěný v kyselině chlorovodíkové. Reakcí pracovního roztoku s vápenatými ionty vznikne komplex kresolftalexonvápník, který se stanoví fotometricky. Znásob stanovení ,ie vhodný především pro stanovení vápníku v biologickém materiálu a ve vodě.The invention relates to a method for the determination of calcium, mainly in biological material and in the water. The nature of the calcium determination method The present invention is based on being examined of the sample, add 1-5 ml of working solution, of the same parts of the chromogen monoethanolamine buffer. Chromogen contains cresolftalexone and 8-hydroxichinoline dissolved in hydrochloric acid. Reaction of working solution with calcium ions will form a complex cresolphalexonvaporant, which is determined photometrically. Multiple determinations, ie especially suitable for the determination of calcium in biological material and in the water.
Description
Vynález se týká způšobu stanovení koncentrace vápníku, především v biologickém materiálu a ve vodš.The invention relates to a method for determining the concentration of calcium, in particular in biological material and water.
V současné době existuje velký počet metod pro stanovení vápníku, počínaje metodami klasickými přes metody fotometrické až k moderní instrumentální analýze, jež je reprezentována atomovou absorpční fotometrií.There are currently a large number of methods for the determination of calcium, ranging from classical methods to photometric methods to modern instrumental analysis, which is represented by atomic absorption photometry.
K fotometrickému stanovení vápníku se používá řada činidel, mezi než patří i kresolftalein-koraplexon.A number of reagents are used for the photometric determination of calcium, including cresolftalein-coraplexone.
IAND
Dosud používané fotometrické metody, jež jsou používány ke stanovení vápníku, mají řadu nevýhod. Především jde o nedostatečnou linearitu, nebot jsou linární do 3,0 mmol/l. Další nevýhodou těchto metod je nízká citlivoat a s tím související vysoká hodnota variačního koeficientu jako kritéria reprodukovatelnostl. Největším nedostatkem dosud používaných fotomerických metod je vysoká hodnota blanku, jež má za následek, že fotomerická měření na běžných fotometrech Spekol nelze provést.The photometric methods used so far, which are used to determine calcium, have a number of disadvantages. Above all it is insufficient linearity because they are linear up to 3.0 mmol / l. Another disadvantage of these methods is the low sensitivity and the associated high coefficient of variation as a reproducibility criterion. The biggest drawback of the photomeric methods used hitherto is the high blank value, which results in photomeric measurements on conventional Spekol photometers cannot be performed.
VIN
Uvedené nedostatky známého stavu techniky odstraňuje způaob stanovení podle vynálezu.These drawbacks of the prior art are overcome by the method of the invention.
Podstata způsobu stanovení vápníku podle vynálezu spočívá v tom, že se ke zkoumanému vzorku přidá 1 až 5 ml pracovního roztoku, tvořeného etejnými díly chromogenu, tvořenéhokresolftalein-komplexonera a 8-hydroxichinolinem, a monoetanolaninového pufru, čímž vzni kne komplex kresolftalein-komplexonu s vápníkem, kter?' se stanoví fotometričky.The principle of the calcium determination method according to the invention consists in adding to the sample to be examined 1 to 5 ml of a working solution consisting of equal parts of chromogen consisting of cresolphthalein-complexonera and 8-hydroxyquinoline and a monoethanolanine buffer to form knee cresolphthalein-complexone with calcium. which? ' the photometers are determined.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS585780A CS216388B1 (en) | 1980-08-27 | 1980-08-27 | Method of determination of the calcium concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS585780A CS216388B1 (en) | 1980-08-27 | 1980-08-27 | Method of determination of the calcium concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS216388B1 true CS216388B1 (en) | 1982-10-29 |
Family
ID=5404288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS585780A CS216388B1 (en) | 1980-08-27 | 1980-08-27 | Method of determination of the calcium concentration |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS216388B1 (en) |
-
1980
- 1980-08-27 CS CS585780A patent/CS216388B1/en unknown
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