CS200010B1 - Chamber for potassium flameless measurement for example in the serum,blood and plasma - Google Patents

Description

The invention relates to the accurate determination of potassium in biological fluids during surgery. Normally in laboratory operation the potassium content is determined in serum, plasma - blood after separation of erythroats by centrifugation in undiluted solutions by flame photometer. More recently, ion-selective electrodes for the direct determination of potassium are introduced without diluting the sample. A number of instruments have been developed for this purpose, including the well-known ORION »SS - 30 analyzer, manufactured by Orion Biomedical, Inc., Cambridge, Mass. For potentiometric analysis, a valinomycin electrode is used herein to directly analyze potassium in blood or plasma (Jack H. Landensont Clinical Chemistry, Vol., 23, No, 10, 1977). Existing products are inexpensive, technically demanding.

The essence of the invention is the rapid and flameless determination of potassium in biological fluids in a closed system consisting of a cell with two exchangeable electrodes.

The design of the cell according to the invention is easy to manufacture and connect to any laboratory pH meter for measurement. The washing and mechanical cleaning of the chamber interior and the regeneration of the Ion bridge are greatly simplified.

In a two-stage conical base on rubber pads that alleviate displacement of the chamber during filling, a measuring chamber with a potassium ion selective electrode and a salt bridge is tightly inserted.

The surface of the ion bridge in the measuring proethor is sufficient to ensure perfect

200 010

200 010 contact with the measured biological fluid. The perpendicular position of the filling of the bridge column eliminates its failure. Any changes in the measured fluid can be easily observed in the transparent chamber material.

The measuring chamber uses direct contact of both electrodes with the measured medium to achieve a conductive connection. The main part of the salt bridge is easily replaceable. Both electrodes are housed in rubber sealing rings and secured by screw flanges which, after tightening, prevent leakage of fluid from the measuring rooms. The upper part of the salt bridge column is provided with a tap which is closed during measurement. By opening it, an easy and quick regeneration of the surface of the fry at the lower end of the salt bridge is achieved, with the liquid from the upper reservoir discharged by the stopcock. In the second branched portion of the salt bridge there is room for a commercial calomel electrode. The measured biological fluid ae is injected into the filling chamber from a syringe in the lower part of the chamber, where a needle with a syringe adapter is inserted and terminated in the center of the measuring space. The syringe remains connected to the container during measurement. This construct is suitable for measuring the amount of potassium in 1 ml of biological fluid. Mixing the fluid while filling the measuring space by compressing the plunger of the syringe is very effective. It is also not a problem that when washing the measuring chamber and regenerating the salt bridge, the area is slightly contaminated by a salt bridge solution, which is of different composition and therefore does not matter.

The replaceable bridge container described herein removes a number of disadvantages of said apparatuses in that the fluid compartment is formed by a roll that facilitates perfect mixing and a small blind retention space. The inner part is therefore well cleaned and dried. The bottom of the container is conical and the outlet opening is in the lowest part so that the flushed solution can be completely removed from the container.

Example of embodiment according to the drawing

In a two-stage conical pedestal provided with rubber pads 2 is a chamber 3 of transparent material with a vertical measurement space 5 with an injection port 6 in the lowest part, the chamber 3 in the bottom part having a recess for the selective electrode 7 on one side and a minimum distance on the opposite side a replaceable ion bridge 10 having a minimum surface area smaller than the membrane surface of the selective electrode 7, the replaceable ion bridge 10 being resiliently coupled to the ion bridge 15 ypsilon-branched, in which the upper branch of the vertical branch has a reservoir 16 with inlet valve 14 and second branch 8 The ion-selective electrode 7 is embedded in a sealing ring 8 compressed by a flange 9, which can be screw-like and also replaceable frit of the ion bridge 10 is housed in a seal 11 compressed naturally. ubou 12.

The workload is minimal and the device can be connected to a commonly used ρΗ-meter in a clinical laboratory.

The machine is easy to manufacture, easy to clean, and customized to suit the content. The whole measurement procedure can be monitored. The electrodes are well removable and perfectly sealed. Sealing flanges prevent the flow of the measured fluid. The ion bridge frit is perhaps3

200 010 no replaceable for parts of different permeability. The entire system is trouble-free and easy to operate, easy to maintain and trained staff. The placement of the selective electrode does not disturb the equilibrium of the whole device, which is very stable as a whole. This example does not exhaust all possibilities of design and use of the device.

Claims (2)

SUBJECT OF THE INVENTION Chamber for flameless measurement of potassium in biological fluids, characterized in that a two-step conical base (1) provided with rubber pads (2) is fitted with a chamber (3) of transparent material with a vertical space (5) for the measured fluid with injection an opening (6) in the lowest part, the chamber (3) at the bottom having a recess for the selective electrode (7) on one side and a replaceable ion bridge (10) frit having a minimum surface area smaller than the membrane area a selective electrode (7), wherein the replaceable ion bridge frit (10) is resiliently connected to the ion bridge (15) in a ypsilon-branched manner, at the top of the vertical branch being a reservoir (16) with an inlet tap (14) and 17) is mounted on a stand (18). 2. The cell of claim 1, wherein the ion-selective electrode (7) is housed in a sealing ring (8) compressed by the flange (9) and also the replaceable ion bridge frit (10) is housed in the sealing (11) compressed by the flange (9). 12).