CN86108878A - Method for Extracting Nutrients from Pollen Using Enzyme - Google Patents
Method for Extracting Nutrients from Pollen Using Enzyme Download PDFInfo
- Publication number
- CN86108878A CN86108878A CN 86108878 CN86108878A CN86108878A CN 86108878 A CN86108878 A CN 86108878A CN 86108878 CN86108878 CN 86108878 CN 86108878 A CN86108878 A CN 86108878A CN 86108878 A CN86108878 A CN 86108878A
- Authority
- CN
- China
- Prior art keywords
- pollen
- enzyme
- protein
- human body
- amino acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 25
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims description 24
- 235000015097 nutrients Nutrition 0.000 title claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 150000001413 amino acids Chemical class 0.000 claims abstract description 9
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 5
- 229940088598 enzyme Drugs 0.000 claims description 23
- 239000004365 Protease Substances 0.000 claims description 20
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 235000019419 proteases Nutrition 0.000 claims description 9
- 108090000526 Papain Proteins 0.000 claims description 6
- 229940055729 papain Drugs 0.000 claims description 6
- 235000019834 papain Nutrition 0.000 claims description 6
- 108010004032 Bromelains Proteins 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 235000019835 bromelain Nutrition 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 235000013361 beverage Nutrition 0.000 abstract description 5
- 230000015271 coagulation Effects 0.000 abstract description 2
- 238000005345 coagulation Methods 0.000 abstract description 2
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 239000003205 fragrance Substances 0.000 abstract description 2
- 235000012041 food component Nutrition 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000035622 drinking Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LNSXRXFBSDRILE-UHFFFAOYSA-N Cucurbitacin Natural products CC(=O)OC(C)(C)C=CC(=O)C(C)(O)C1C(O)CC2(C)C3CC=C4C(C)(C)C(O)C(O)CC4(C)C3(C)C(=O)CC12C LNSXRXFBSDRILE-UHFFFAOYSA-N 0.000 description 1
- CVKKIVYBGGDJCR-SXDZHWHFSA-N Cucurbitacin B Natural products CC(=O)OC(C)(C)C=CC(=O)[C@@](C)(O)[C@@H]1[C@@H](O)C[C@]2(C)C3=CC[C@@H]4C(C)(C)C(=O)[C@H](O)C[C@@]4(C)[C@@H]3CC(=O)[C@@]12C CVKKIVYBGGDJCR-SXDZHWHFSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 150000001904 cucurbitacins Chemical class 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- PIGAXYFCLPQWOD-UHFFFAOYSA-N dihydrocucurbitacin I Natural products CC12C(=O)CC3(C)C(C(C)(O)C(=O)CCC(C)(O)C)C(O)CC3(C)C1CC=C1C2C=C(O)C(=O)C1(C)C PIGAXYFCLPQWOD-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000014594 pastries Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 229940109850 royal jelly Drugs 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
本发明是利用酶的催化作用，使花粉中的蛋白质转变为易被人体吸收的多种氨基酸。用本发明得到的花粉提取液容易过滤，无青草腥味，可以直接配制饮料，并保持花粉原有的清香风味和其他营养成份。不存在花粉提取液受热产生蛋白质凝固的现象。The invention uses the catalysis of enzymes to transform the protein in the pollen into various amino acids that are easily absorbed by the human body. The pollen extract obtained by the invention is easy to filter, has no grassy smell, can directly prepare beverages, and maintains the original fragrance, flavor and other nutritional components of the pollen. There is no phenomenon that the protein coagulation occurs when the pollen extract is heated.
Description
The present invention relates to a method for extracting nutrient components from pollen by using enzyme.
There are two types and four methods for extracting nutrients from pollen so far. The first type is the physical extraction of nutrients from pollen. It features that the molecular structure of original nutrients of pollen is kept unchanged. This type contains three methods: namely a mechanical wall-breaking warm water extraction method, a temperature difference extraction method and a solvent extraction method. The nutrient components of the pollen extract obtained by the method are mainly pollen protein which can be absorbed by human body only through the action of enzyme in the human body. The pollen extract is difficult to filter and has low product yield. If the beverage is prepared by using the protein, the phenomenon of protein solidification can be generated in the process of heating sterilization, and the appearance and the quality of the product are seriously influenced. Therefore, only pastry, food and cosmetics can be made with it. The second type is that the protein in pollen is converted into various amino acids which are easily absorbed by human body directly by means of biochemical reaction. The prior art for this type of process is a fermentation process. In the method, the reproduction and growth of microorganisms need to consume part of nutrients of pollen in the fermentation process, so that the yield is low. Meanwhile, the production period is long (the solid fermentation period is 10-20 days, and the liquid fermentation period is 4-5 days).
The task of the invention is to research a new method for extracting nutrient components from pollen. The method has short production period and high product yield, and the nutrient components of the extracting solution are various amino acids easy to be absorbed by human body.
The present invention features that the catalytic action of enzyme is utilized to convert the protein in pollen into amino acids easy to be absorbed by human body. The enzymes selected may be: enzyme preparations such as papain, bromelain, bacterial protease or mixed protease. The invention is to put the pollen material into a container with stirring, soak the pollen material with warm drinking tap water, fully wet the pollen cells, and adjust the pH value and temperature of the material in the container to meet the condition requirements of enzyme catalytic reaction. Adding enzyme preparation, stirring, and allowing the materials in the container to start enzyme catalysis reaction, so that the protein in pollen is gradually converted into various amino acids. Heating to kill enzyme after reaction (for some food needing enzyme preservation, other disinfection methods can be adopted, and enzyme killing is not needed). Filtering to remove the residue. The pollen extract with high amino acid content is obtained, and the amino acid content is shown in the attached table.
The present invention utilizes the catalytic action of enzyme to convert the protein in pollen into several amino acids easy to be absorbed by human body. The nutrient components of pollen are not lost in the process of enzyme catalysis reaction, so that the extraction method has high yield. The enzyme reaction speed is high (for this fermentation method), so the production cycle is short, generally about eight hours. The pollen extract obtained by the invention is easy to filter, can effectively remove the green smell of pollen, does not have the phenomenon of protein coagulation caused by heating of the pollen extract, and can be directly used for preparing beverages. The beverage can retain the original fragrance and flavor of pollen and other nutrients.
Example one
1. Adding pollen material into a container with stirring, and soaking in 50 deg.C drinking tap water for 2 hr. The proportion of the added water is 1: 4-5. After fully stirring, adding 40 ℃ drinking tap water until the ratio of pollen to water is 1: 8-10.
2. And adjusting the pH value of the material in the container to be about 6 and the temperature to be 40-50 ℃.
3. Adding the cucurbitacin. The enzyme addition amount is 0.1-0.2% of the amount of the pollen (dry basis).
4. Stirred well for 5 hours.
5. Heating to kill enzyme, heating the container to 100 deg.C, and stopping heating.
6. Filtering with a plate-frame filter while hot.
The nutrient components of the obtained pollen extract are shown in the data of the column of papain in the attached table. Such an extract can be used as: directly preparing pollen beverage, pollen, royal jelly, etc., adsorbing the extracted liquid with adsorbent (powdered sugar or wheat flour, etc.), drying and granulating to obtain solid powdered product, such as pollen extract, etc.
The obtained residue can be used for preparing cake food, etc.
Example 2
The operation method and the process conditions are the same as those of example 1. Only the enzyme species is added, and the papain is changed into the bromelain. The nutrient components of the obtained pollen extract are shown in the data of bromelain column in the attached table.
Example 3
The operation method and the process conditions are the same as those of example 1. Only the enzyme species added was changed from papain to bacterial protease. The nutrient contents of the obtained pollen extract are shown in the data of bacterial protease column in the attached table.
Example 4
The operation method and the process conditions are the same as those of example 1, and only the enzyme type is changed from papain to mixed protease (i.e., mixed enzyme of bromelain and bacterial protease). The nutrient contents of the obtained pollen extract are shown in the column of mixed protease in the attached table.
Attached table of the specification
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86108878 CN86108878A (en) | 1986-12-30 | 1986-12-30 | Method for Extracting Nutrients from Pollen Using Enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86108878 CN86108878A (en) | 1986-12-30 | 1986-12-30 | Method for Extracting Nutrients from Pollen Using Enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN86108878A true CN86108878A (en) | 1988-07-20 |
Family
ID=4804087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86108878 Pending CN86108878A (en) | 1986-12-30 | 1986-12-30 | Method for Extracting Nutrients from Pollen Using Enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN86108878A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN105214335A (en) * | 2015-10-16 | 2016-01-06 | 新疆于田瑰觅生物科技股份有限公司 | The method of pollen aminoacids solution is extracted in Flos Rosae Rugosas pollen |
-
1986
- 1986-12-30 CN CN 86108878 patent/CN86108878A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN102229973B (en) * | 2011-06-15 | 2013-05-01 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN105214335A (en) * | 2015-10-16 | 2016-01-06 | 新疆于田瑰觅生物科技股份有限公司 | The method of pollen aminoacids solution is extracted in Flos Rosae Rugosas pollen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |