CN85108913A - Microbial plant growth promoter and yield enhancer - Google Patents
Microbial plant growth promoter and yield enhancer Download PDFInfo
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- CN85108913A CN85108913A CN 85108913 CN85108913A CN85108913A CN 85108913 A CN85108913 A CN 85108913A CN 85108913 CN85108913 CN 85108913 CN 85108913 A CN85108913 A CN 85108913A CN 85108913 A CN85108913 A CN 85108913A
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Abstract
Disclose comprising of a kind of novelty a kind of bacterium and the microbial plant growth of algae mixture promote composition, and utilize its to promote a kind of method of plant growing.
Description
The present invention relates to promote the composition and the method for plant growing.More particularly, the present invention relates to microbial plant growth and promote compoistion and method of use.
Since the mankind begin cultivated plant at first, be concerned about the growth that promotes plant always and improved output.This is particularly important for having only the determinate growth boreal climate in season.
In the trial that promotes plant growing, all different approach had been sought.Using microbe reaches this result and extremely attracts people's attention always, particularly uses azotobacteria.Designed the whole bag of tricks plant roots is contacted with azotobacteria, comprised and this bacterium being transplanted in soil and with the compositions-treated seed that contains bacterium.
Except azotobacteria, it is believed that other microorganism of finding in the soil is also beneficial to plant growing.Although also do not know the mechanism that this beneficial effect is definite, existing people thinks that these microorganisms are decomposed into the complex component in the soil and can be the nutrient that plant assimilated.The condition that is of value to growth of microorganism in the jungle soil is considered to the partly cause that this area's thriftiness is grown.
Therefore that is the growth of improvement plant algae also has been used to improve soil condition.4,336, No. 051 United States Patent (USP) as Marguez has been described the soil improvement composition that contains agar type seaweeds.In the purpose that these compositions are specified, comprise the growth that produces useful enzyme and suppress malignant bacteria competitively.
Because current and foreseeable needs to the world food supply, there is continual interest in the ways and means that improves the plant production rate for development.
According to the present invention, a kind of microbial plant growth promotes composition to include the mixture of a kind of bacterium and a kind of algae.Also disclose by will promote comprising of plant growing amount a kind of bacterium and the composition of algae mixture put on plant or its environment, promote the method for plant growing.
It has surprisingly been found that bacterium and algae, or their accessory substance, according to the present invention in conjunction with and when using, have the effect that promotes growth after putting on plant or its environment.
Show that composition of the present invention is grown up strong and sturdy in early days for plant and plant products has important good effect.Further, use composition of the present invention to promote fruit tree growth, than this plant species of not using the said composition growth, can make the faster listing in advance of more fruit.This is for especially useful the cultivation under boreal climate, and there, results are very important earlier.
Bacterium used in the present invention is Escherichia (Escherichia) and bacillus (Bacillus) bacterium preferably, and bacillus subtilis (B.Subti-lis) is because its non-pathogenic is particularly desirable.The algae component of composition is that green alga (Chlorophyta) portion is more favourable, and Chlorococcum (Chlorococcales) purpose algae is better.The algae of Chlorella (Chlorella) is particularly desirable, has a liking for sugared chlorella (Chlorellasaccharophilia) for best.
The bacterium of the present composition and algae component, preferably preparation respectively in suitable nutrient medium under growth conditions combines them then, cultivates in nutrient medium to produce end product under growth conditions.Growth conditions comprises physiologically acceptable pH value and temperature.Contain assimilable carbon source, nitrogenous source and basic trace mineral, vitamin and growth factor in the nutrient medium.Various sugar can be used as suitable carbon source as lactose, glucose or natural carbohydrate such as syrup etc. and use.Inorganic ammonium salt is as ammonium nitrate, ammonium sulfate, ammonium phosphate etc. and egg white icing, as meat soup, tryptose
Soup, soybean meal etc. can provide nitrogenous source.The chlorate of mineral salt such as sodium, potassium, calcium, magnesium, molybdenum, sulphate, phosphate etc. can be used to provide desired trace mineral.In addition, basic growth factor is preferably provided by the commercially available prepared product that gets as vitamin, as yeast extract etc.The best nutritional medium of preparation bacterium and algae component is described in the following examples 1.
Long behind suitable cell density in bacterium and algal cultures, as for bacterial cultures, from about 2 * 10
6To 10 * 10
6, preferably about 4 * 10
6To about 6 * 10
6Individual cells/ml is for algal cultures, from about 10 * 10
6To about 20 * 10
6, preferably about 14 * 10
6To about 16 * 10
6Individual cells/ml mixes two kinds of cultures.In the mixture of gained, add other nutrient again, under growth conditions, cultivate this mixture.The most handy whey is as nutrient source, and those of ordinary skills can know clearly can use as the front and say other carbon source, nitrogenous source, mineral matter and growth factor being.Medium preferably also comprises the Sores-epinum (yucca (Yucca) extract obtains from California, USA Los Angeles Richter scale Brother Internat Corp.) of a stable quantity.The concentration of Soresepinum is with about 0.5% to about 2% for well, and is better between about 1% and about 1.5%, and be about 1.25% to be the best with concentration especially.In the training period, being preferably in the mixture also to add is enough to stablize this mixture and the unlikely lipase that makes its decomposition amount, and lipase concentration is with about 0.5% to about 2% for well, and from about 1% to about 1.5% is better, and be the equal by volume of about 1.25%(with concentration especially) be the best.In general, mixture is at about 15 ℃ to about 40 ℃, preferably cultivates about 1～4 week to about 25 ℃ temperature from about 20 ℃, preferably 2～3 week.
Carry out acidifying with a kind of non-toxic acid such as lactic acid or acetate and stop the growth of cell, can stablize the bacterium-algae composition of this gained like this.PH value drops to about 3～5 for well, and preferably about 4.0.The preservative that also will add anticorrosion amount is as Sodium Benzoate.Composition also contains the vitamin B-12 of stable quantity preferably.Particularly preferably, the amount that adds the vitamin B-12 in the composition is in from about 1.5 gram/gallons to the scope of about 5 gram/gallon compositions, and addition is with about 2 gram/gallon composition the bests.
The invention still further relates to and use this microbial plant growth to regulate a kind of method that composition increases plant growing.The better method of handling plant is for irrigating or spraying.Perhaps, also available said composition is handled seed, preferably seed is soaked in said composition about 2 to 24 hours prior to seeding.To serve as to promote the amount of growth to use said composition with one.This quantity can be along with different usings method, weather and soil condition, and related specific crop and changing.In general, every acre is used about 1 pound to 3 pounds, preferably every acre about 1.5 pounds to about 2 pounds.
In the application of solution cultivating method, said composition directly is added in this nutrient solution.About 0.5 to the 3.0 milliliter of microbial composite of the preferably per 80 gallons of nutrient mediums of the ratio of microbial composite and nutrient solution.
Microbial plant growth of the present invention is regulated composition can directly be sprayed onto (blade face application) on the leaves of plants, to improve plant growing and output.Use for the blade face, common every acre is used about 0.2 pound to about 1 pound, preferably every acre about 1/4 pound to about 1/3 pound.Preferably, mix, make composition be diluted to a suitable concentration, be applied on the blade face equably to be easy to said composition as water with said composition and an appropriate carriers.In order to promote the absorption of blade face for said composition, can one or more be a spot of by adding (as, up to by weight percentage about 5%) in agronomy industry for material being put on the nontoxic leaf surfaces activating agent of using usually on the plant leaf, or other nontoxic blade face bleeding agent or wetting agent.Suitable sorbefacient additives can comprise nontoxic anion, cation and non-ionic surfactant, humus or derivatives thereof and polyethylene glycol.Composition according to this this embodiment preferably includes some additional plant nutrients, as assimilable nitrogen (N), phosphorus (P) and potassium (K), for example, with 14: 7: 3 N-P-K ratio.Said composition preferably also comprise on a small quantity (as, by weight percentage 0.1～0.5%) preferably with the trace element that forms chelate as ethylenediamine tetra-acetic acid (EDTA), as molybdenum, selenium and boron.Can add other a spot of Soresepinum(as, by weight percentage 0.01～0.1%) in the said composition to increase stability.
To further specify the present invention with more following embodiment, but they are not limitation of the present invention.
According to follow procedure, prepare bacillus subtilis respectively and have a liking for the culture of sugared chlorella, combine them then and handled to produce end product.
The preparation of bacterial cultures component
The sample of bacillus subtilis (is obtained ATCC from Maryland State Luo Keweile U.S. typical case's culture collection center
#6461) join by containing percent by volume and be approximately 3.5% lactose, PH is 6.8 to 7.0 lauryl tryptose
Soup (Difco
#0241-02-7, Michigan Detroit city Di Fuke) in the enriched medium of forming.This mixture was cultivated 24 hours under 35 ℃ of positive and negative temperature of 1 ℃, to finish CO
2Displacement to lactose.The pH value of this mixture dyestuff (bud green laboratory bile, the Difco of PH sensitivity
#007-01-2) be adjusted between 6.5 to 6.8.This mixture was cultivated 24 hours under 35 ℃ of positive and negative temperature of 1 ℃ then.
The separation of this culture nutrient agar (Difco
#0001-02) finish, this medium is made up of the beef extract of 3 grams per liters, the peptone (Difco) of 5 grams per liters and the agar of 15 grams per liters, and PH is 6.8 to 7.0.This mixture was cultivated 24 to 48 hours in water-bath under 44.5 ℃ of positive and negative temperature of 0.1 ℃ then, until the number that reaches 5,000,000 cells/ml.
The preparation of algal cultures component
To have a liking for sugared chlorella sample and (obtain ATCC from Maryland, USA Luo Keweile typical case culture collection center
#30408) join algae
In the agar medium, this medium is by 15 to 20 grams per liter agar, 0.2 to 0.3 grams per liter sodium nitrate, 2 grams per liter calcium chloride, 0.5 grams per liter magnesium sulfate, 15 grams per liter potassium phosphates, 3.0 grams per liter sodium chloride, 7.0 to 8.0 grams per liters
Peptone, 0.2 grams per liter molybdenum trisulfate are formed, and at PH6.5 to 6.8 and 1 liter of deionized water balance.This mixture cultivated for 2～3 week in sunlight under 22 ℃ to 25 ℃ temperature.
Mixture is transferred to by above-mentioned algae
Agar medium is formed, but does not have in the special culture medium of molybdenum trisulfate 2～3 days, with the separation and Culture thing and reach 15,000,000/ milliliter number.This number is measured by microscopic counting.Then that culture is centrifugal, isolate the liquid part and also this liquid is partly discarded.
Microbial plant growth is regulated the preparation of composition (biological reagent)
Above-mentioned bacterium for preparing and algal cultures are mixed, and tale reaches 20,000,000/ milliliter (15,000,000/ milliliter algae and 5,000, bacterium of 000/ milliliter).9 gallons of 1 gallon of mixture addings are added with in the whey of 1 pint of Soresepinum, and under 20 ℃ to 22 ℃ temperature, cultivated for two to three weeks.Every day, four stir culture things and inspection were counted.(be about the cycle mid point) after 10 days and add 1 product defatting enzyme (Difco
#0431-63-3).At the cultivation cycle terminal point, pH value is adjusted to 4 positive and negative 0.2 and add Sodium Benzoate to 5ppm concentration with the lactic acid titration.Last per gallon adds the vitamin B-12 composition (Difco of 2 grams
#6300-15-7) to produce end product.This end product keeps in Dark Place under 65 °F at 55 °F.
Carrying out following experiment is to increase the ability of tomato growth in order to measure the microbial composite of describing among the top embodiment 1 (biological reagent).These experiments are carried out on two levels.A test utilizes the bag system to carry out in a commodity greenhouse, has used the mixture of bog moss and vermiculite in this bag system.Another test utilizes the growth room to carry out, and plant grows in water under a constant environment condition short period therein.In the growth room system, biological reagent adds in the nutrient solution every day; And in bag was cultivated, the biological reagent jede Woche added twice.
1, greenhouse
Seed is handled the back sowing with biological reagent.After one week, 50 percent seedling is sprouted.After rough leaf occurs, with the water pouring seedling of 1/4 ounce of fertilizer concentration of per gallon.This situation keeps a week, uses the water of 1/2 ounce of fertilizer concentration of per gallon then.After one month, seedling is moved into the bag culture systems.During the initial fortnight of growing in bag, the electrical conductivity of nutrient solution rises to 1.8 from 1.0.The pH value of nutrient solution remains on about 6.5.With the plant that biological reagent is handled, jede Woche accepts to contain in per 50 gallons for twice the nutrient solution of 1.5 milliliters of biological reagents.
Containing bog moss: the plant growing in the vermiculite mix bag demonstrates the extremely long-pending reaction that adds biological reagent for this.In Fig. 1, obviously improved by its growth rate of highly measuring by the plant that biological reagent is handled.Table 1 has shown the leaf that grows in plant in the biological reagent and the increase of stem fresh weight.Table 2 shows, in the plant that biological reagent was handled, ties in the average number of first bunch fruit highlyer, and the weight of each fruit is also bigger.These data show, than growing plants does not have the size that more fruit reaches listing sooner in the plant that biological reagent was handled in there being biological reagent.
2, growth room
In these experiments, the tomato seed is sprouted under constant water smoke in vermiculite.When the seedling rough leaf has 2 centimeter length, they are shifted out and wash its root from vermiculite.Seedling put into contain 750 milliliters of nutrient solutions (electrical conductivity 1.8 is in the jar of 1 liter of PH6.5) some.With a little pump air is pressed in the solution, makes the constant ventilation of root.Every day accepted biological reagent by the container of half, and second half is not.The nutrient solution level adds new soln during by needs and remains between 700～750 milliliters.Nutrient solution is changed once weekly.These seedlings are put into after the nutrient solution, and growth is about 21 days in the growth room.
Two batches of experiments have been carried out.Table 3 has comprised the data that drawn by first test.Second batch of test for data is included in table 4 and the table 5.Again as first group the test in situation, the result has shown positive biological reagent effect.The dry weight of leaf, stem and root is owing to using biological reagent to increase by 10～20%.Photosynthetic rate has risen 29%, and the leaf area has risen 12.5%.
Table 1, biological reagent are to the comparison of cauline leaf fresh weight effect.These data are to measure and represent the mean value of 10 plant at last.
Handle leaf fresh weight (gram) stem fresh weight (gram)
Bag is cultivated (+) biological reagent 227.2 102.3
Bag is cultivated (-) biological reagent 223.5 93.3
Table 2, biological reagent are to the effect of plant products.These data are to measure and represent the mean value of 10 plant at last.
Handle fruit number/bunch total fresh weight (gram) weight/fruit
Bag is cultivated (+) biological reagent 3.0 654 21.8
（+25%） （+53.8%） （+23.1%）
Bag is cultivated (-) biological reagent 2.4 425 17.7
Table 3, the effect (4 plant average) of biological reagent to growing in the tomato seedling in the growth room.
Handle photosynthetic rate (milligram CO
2/ hour) average plant leaf area (square centimeter)
Biological reagent 510(+51.7%) 242.19(+14%)
Contrast 336 209.45
Fresh weight
The blade root stem
Biological reagent 3.6(+0%) 3.70(+15% 0.46(+35%))
Contrast 3.6 0.3 3.17
Table 4, growth room's experiment.Biological reagent is to the effect of accumulation dry weight
Handle the base of leaf root
Biological reagent 0.28 gram 0.09 gram 0.06 gram
（+27.2%） （+12.5%） （+20%）
Contrast 0.22 gram 0.08 gram 0.05 gram
Table 5, growth room's experiment.Biological reagent is to the effect of photosynthesis and leaf area.
Handle photosynthetic rate (milligram CO
2/ hour) the average plant leaf area
Biological reagent 880(+41.9%) 274.1 square centimeter
（+12.5%）
Contrast 620 240.3 square centimeters
Carry out following experiment, be for illustrate resemble described in the embodiment 1 and the microbial composite (biological reagent) of preparation to the effect of tomato plant strain growth in nutrient film technology (NFT) the hydroponics growing system.
Program:
In these experiments, it is different that the amount (volume) of the microbial composite in the adding nutrient reservoir reaches the number of times that adds this biological reagent weekly.Adopted following processing mode: handle the 1-experimental session and add 2.4 milliliters of microbial composite/80 gallon nutrient solutions every day.
Processing 2-begins fortnight and adds 2.4 milliliters of microbial composite/80 gallon nutrient solutions, 1.2 milliliters of microbial composite/80 gallon nutrient solutions of twice adding of jede Woche in experimental session after this interior every day.
Processing 3-begins fortnight and adds 2.4 milliliters of microbial composite/80 gallon nutrient solutions interior every day, at 0.6 milliliter of microbial composite/80 gallon nutrient solution of twice adding of experimental session jede Woche after this.
Processing 4-begins fortnight and adds 0.6 milliliter of microbial composite/80 gallon nutrient solution interior every day, in fortnight after this, and 1.2 milliliters of microbial composite/80 gallon nutrient solutions of twice adding of jede Woche; In experimental session after this, 2.4 milliliters of microbial composite/80 gallon nutrient solutions of twice adding of jede Woche.
Handle the 5-contrast; Do not add microbial composite.
Each processing is made up of four 6 inches wide NFT groove and one 80 gallons nutrient solution reservoir.Each handles used cultural method all is similar, and then carries out cultivating in NFT the normal running program of commercially available tomato crop." Jumbo " kind is used in this whole research.Sow seed, after seedling is moved in the groove, allow its grow again 48 days.Begin to add microbial composite behind the fortnight.
The result:
The effect that various processing is nourished and grown to tomato can be found out in Fig. 2, Fig. 3 and Fig. 4 and Table A.Fig. 2 shows that after beginning to add microbial composite, each processing method does not in height have or few of difference.Yet under the different disposal mode only behind the fortnight, the plant that biological reagent was handled is significantly higher than check plant.This reaction also can find out from Table A, and in each situation of Table A, the fresh weight of the leaf of the plant that biological reagent was handled, root and stem all is greater than check plant.Compare with control group, as if it is big how much length of the plant roots that biological reagent was handled also wants.
Fig. 3 also points out, the average leaf area of the plant that biological reagent was handled under each situation is all wanted significantly greater than check plant.This figure shows that also the stimulation of handling 4 pairs of leaf growths is greater than other biological reagent processing.Processing method 4 and 1 diameter stem are tested the much bigger this point that also shown of processing method than other among Fig. 4.
Fig. 5 (A and B) shows, compares with control group, and handling 1,2 and 3 has stimulated the two the growth of total fruit number and total fruit weight.
Fig. 6 (A and B) has provided the data of the gross yield of initial adding microbial composite after about 12 weeks.As can be seen, processing method 1,2 and 3, especially processing method 2, and therefore reproductive development is faster and more early begin to produce the fruit that can go on the market.
Table B and table C have also comprised and have related to the data of effect of microbial composite to growth.Distance (table B) between these processing methods are tended to increase bunch.Microbial composite obviously promotes the early development of fruit, and especially for first output its compelling effect (Fig. 7 A) is arranged. Processing method 1 and 2 has greatly increased the output (Fig. 7 A) of first bunch of fruit that can go on the market.Yet, afterwards bunch in (2～6), handling 3 and 4 is (Fig. 7 B) that spread effect is arranged.As if use the processing 1 and 2 of relatively large biological reagent to stimulate the ability of producing in early days, and the 3 and 4 more a spot of uses that provide are provided, may be in whole growth promote in the cycle the to go on the market raising of gross yield.
The effect that Table A, microbial composite are nourished and grown to tomato (kind " Jumbo ").Data are collected after beginning to handle fortnight, and each data is the mean value of 4 plant.
Fresh weight (gram)
Processing base of leaf root root length (centimetre)
1 26.3 9.6 15.2 62.8（+54.67%）
2 23.6 8.4 12.2 43.8（+7.88%）
3 28.8 9.9 13.0 44.2（+8.86%）
4 31.5 11.2 12.2 52.7（+29.80%）
The 5(contrast) 17.4 5.7 7.6 40.6
Table B, microbial plant growth composition are to the influence of average distance between flowers
Distance between bunch (centimetre)
Bunch handle number
1234 5(contrast)
0-1 25.7 24.2 28.2 28.7 22.2
1-2 21.8 21.4 22.8 18.9 18.0
2-3 17.9 19.9 22.2 18.3 19.3
3-4 22.5 22.2 21.5 18.0 18.3
4-5 15.5 16.6 20.0 16.8 17.2
5-6 13.3 11.4 17.2 18.0 16.9
Table C, benefit from microbial composite and handle average in every bunch of each plant of influence
Spend number.
On average spend to count/bunch/plant
Bunch handle number
1234 5(contrast)
1 5.5 5.3 5.4 4.4 5.2
2 9.9 5.4 7.5 7.2 7.0
3 8.0 7.2 7.9 6.2 6.9
4 6.6 6.1 6.1 6.9 5.4
5 4.9 3.0 5.0 5.4 5.8
6 2.8 4.8 4.4 5.7 4.4
7 5.5 2.0 2.4 4.0 3.3
Amount to 43.2 33.8 38.7 39.8 38.0
Will be according to embodiment 1(preceding) microbial composite (biological reagent) that produces mixes with the vegetable manure of routine, this vegetable manure has carboxylic acid derivates, microelement chelate (reining in the star organic chemical reagent portion of W.R. W. R. Grace ﹠ Co of pausing from Massachusetts, United States obtains), Soresepinum and the water of 14: 7: 3 N-P-K ratio, a kind of humus, to produce following mixture, its percentage by weight for the blade face use is:
Mixture for the blade face use
Total nitrogen (60%) 14%
Phosphoric acid (32%) 7%
Solvable potash (45%) 3%
Humus (carboxylic acid derivates, 13%) 5%
Microelement chelate (30%) 0.5%
Soresepinum 0.05%
Inertia balance material (water) 20.45%
Amount to 100%
The carboxylic acid derivates of humus, be by with two parts of natural rough humus powder composition (Agro-Lig, Illinois, USA Si Keji, U.S. Ke Luode company) 2-with a technical-grade hydrochloric acid that comprises 85% percentage by weight and 15% percentage by weight right-mixture of anisyl ethene bromine mixes compositions-suspension mutually and obtains, humus powder composition wherein is to reach the organic substance exploited in his state of material-make in south from Leonard (Leonardite) shale-a kind of.Allow this suspension react about five week, solvolysis reaction take place around here, 2-is right-anisyl ethene bromine be converted to 2-right-anisyl ethene chlorine, and formed the precipitation of humus powder composition accessory substance.To precipitate product (carboxylic acid derivates) partly separates and as above-mentionedly mixes with biological reagent from liquid.
Shown that the mixture that uses for the blade face has influence to the growth of following plants kind: fragrant English fan (Viburnum odoratissimum), tobira (Pittosp-orum tobria), Chinese holly (Ilex cornuta) " Burfordii ".Once use blade face mixture (2 milliliters/3 gallons water) or tester (0 milliliter/3 gallons water) to spray the blade face at the duration of test jede Woche, be basically covered with blade.Result in table 1 has provided during 4 weeks, the result in table 2 has provided during 12 weeks.
Data show, when the blade face mixture is sprayed on the blade face, have caused than control group significant increase being arranged on plant height, plant weight and root length.
The influence that table 1, processing are all around grown to woody ornamentais
Feature
Heavy (gram) root of botanical name height (inch) plant long (inch)
Blade face mixture 6.8 1.5 36.8 1.1 4.9 0.5
Xiang Ying Opulus
Contrast 3.0 0.3 15.5 2.8 1.8 0.5
Blade face mixture 8.1 1.9 41.5 1.7 6.1 0.2
Tobira
Contrast 3.5 0.3 13.5 2.5 2.5 0.6
Blade face mixture 9.5 2.1 67.5 1.8 6.6 0.3
Chinese holly
Contrast 4.1 0.5 31.3 1.6 2.7 0.5
The processing in table 2,12 weeks is to the influence of woody ornamentais growth
Feature
Heavy (gram) root of botanical name height (inch) plant long (inch)
Blade face mixture 19.5 0.3 126.6 1.3 9.5 0.8
Xiang Ying Opulus
Contrast 6.2 0.5 21.5 1.5 2.5 0.5
Blade face mixture 24.5 0.5 128.3 1.2 11.8 0.3
Tobira
Contrast 6.5 0.3 21.5 0.8 3.5 0.5
Blade face mixture 18.5 2.5 125.5 1.2 12.5 0.5
Chinese holly
Contrast 7.5 0.5 40.8 1.1 4.2 0.3
Claims (32)
1, a kind of preparation microbial plant growth promotes method for compositions, and it is included in cultivates a kind of bacterial cultures in a kind of first nutrient medium under the growth conditions, its cell density is reached from about 2 * 10
6To about 10 * 10
6Individual cells/ml; Under growth conditions, in a kind of second nutrient medium, cultivate a kind of algal cultures, its cell density is reached with about 10 * 10
6To about 20 * 10
6Individual cells/ml is then with bacterium with algal cultures mixes and cultivating this mixture to about 40 ℃ temperature to produce end product at about 10 ℃ under the growth conditions in a kind of the 3rd nutrient medium; Described growth conditions comprises a kind of physiologically acceptable pH value and temperature.
2, the process of claim 1 wherein that the time that the 3rd nutrient medium is cultivated is to about 4 weeks from about 1.
3, by the method for claim 1, a kind of microbial plant growth of generation promotes composition.
4, the composition in the claim 3, wherein said algae are green alga (Chloro-phyta) portion.
5, the composition in the claim 4, wherein said bacterium are that bacillus (Bacillus) belongs to.
6, the composition in the claim 5, wherein said algae are Chlorococcum (Chlorococcales) purpose.
7, the composition in the claim 6, wherein said algae are that chlorella (Chlorella) belongs to.
8, the composition in the claim 7, wherein said bacterium are that bacillus subtilis (B.subtilis) is planted.
9, the composition in the claim 8, wherein said algae are to have a liking for sugared chlorella (C.saccharophilia) to plant.
10, the composition in the claim 3,4,5,6,7,8 or 9, bacterium wherein with every milliliter from about 4 * 10
6To about 6 * 10
6The concentration of individual cell exists, and algae with every milliliter from about 14 * 10
6To about 16 * 10
6The concentration of individual cell exists.
11, the composition in the claim 10, it further comprises Soresepinum, lipase and the vitamin B-12 of stable quantity.
12, the composition in the claim 11, Soresepinum wherein exists with the concentration of percent by volume from about 0.5% to about 2%, lipase exists with the concentration of percent by volume from about 0.5% to about 2%, and vitamin B-12 exists with the concentration of about 1.5 grams of per gallon to about 5 grams.
13, the composition in the claim 10 for the foliar spray use, comprise further being approximately 5% a kind of nontoxic material that up to percentage by weight this non-toxic material is to choose from the group that blade surface activating agent, bleeding agent and wetting agent constitute to plant.
14, the composition for the foliar spray use in the claim 11 further comprises a kind of nontoxic to the plant material up to percentage by weight about 5%, and this non-toxic material chooses from the group that blade surface activating agent, bleeding agent and wetting agent constitute.
15, the composition for the foliar spray use in the claim 12 further comprises a kind of nontoxic to the plant material up to percentage by weight about 5%, and this non-toxic material chooses from the group that blade surface activating agent, bleeding agent and wetting agent constitute.
16, the composition in the claim 13, non-toxic material wherein are humus or its a kind of derivative.
17, the composition in the claim 14, non-toxic material wherein are humus or its a kind of derivative.
18, the composition in the claim 15, non-toxic material wherein are humus or its a kind of derivative.
19, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in claim 3 or 9 is grown applies to described plant.
20, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 10 is grown applies to described plant.
21, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 11 is grown applies to described plant.
22, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 12 is grown applies to described plant.
23, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 13 is grown applies to described plant.
24, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 14 is grown applies to described plant.
25, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 15 is grown applies to described plant.
26, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 16 is grown applies to described plant.
27, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 17 is grown applies to described plant.
28, a kind of method that promotes plant growing comprises that the amount that a promotion of composition in the claim 18 is grown applies to described plant.
29, the method in the claim 19, wherein said composition with about 1 to about 3 pounds/acre amount by irrigating or spraying applies to described plant.
30, the method in the claim 20, wherein said composition applies to described plant with about 1.5 to about 2 pounds/acre amount.
31, the method in the claim 26, wherein said composition applies to described plant by spraying with about 0.2 to about 1 pound/acre amount.
32, the method in the claim 26, wherein said composition applies to described plant with about 1/4 to 1/3 pound/acre amount by spraying.
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|---|---|---|---|
| CN 85108913 CN85108913A (en) | 1985-11-04 | 1985-11-04 | Microbial plant growth promoter and yield enhancer |
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| CN1057563C (en) * | 1997-09-26 | 2000-10-18 | 北京英特莱幸诺生物技术有限责任公司 | Bacterial stain of bacillus subtilis and application thereof |
| CN103300067A (en) * | 2013-07-11 | 2013-09-18 | 唐睿 | Formula and preparation of conduction function type Niannianle nutritional regulator |
| CN103805535A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Isothermal layer bacillus, microbial agent and application of isothermal layer bacillus and microbial agent |
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| CN108293345A (en) * | 2017-01-12 | 2018-07-20 | 喜施倍全球股份有限公司 | Microbe soil enhances |
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- 1985-11-04 CN CN 85108913 patent/CN85108913A/en active Pending
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| CN1057563C (en) * | 1997-09-26 | 2000-10-18 | 北京英特莱幸诺生物技术有限责任公司 | Bacterial stain of bacillus subtilis and application thereof |
| CN103300067A (en) * | 2013-07-11 | 2013-09-18 | 唐睿 | Formula and preparation of conduction function type Niannianle nutritional regulator |
| CN105431397B (en) * | 2013-08-09 | 2020-08-25 | 朝日生物循环科技株式会社 | Method for cultivating crop using bacillus bacteria |
| CN105431397A (en) * | 2013-08-09 | 2016-03-23 | 朝日可尔必思健康株式会社 | Method for cultivating crops using bacteria of the genus Bacillus |
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