CN2761164Y - Biological reactor - Google Patents
Biological reactor Download PDFInfo
- Publication number
- CN2761164Y CN2761164Y CN 200420121031 CN200420121031U CN2761164Y CN 2761164 Y CN2761164 Y CN 2761164Y CN 200420121031 CN200420121031 CN 200420121031 CN 200420121031 U CN200420121031 U CN 200420121031U CN 2761164 Y CN2761164 Y CN 2761164Y
- Authority
- CN
- China
- Prior art keywords
- tank
- culture tank
- main culture
- bioreactor
- constant temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 27
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000741 silica gel Substances 0.000 claims abstract description 17
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000004113 cell culture Methods 0.000 claims abstract description 6
- 229920001296 polysiloxane Polymers 0.000 claims abstract 5
- 239000007788 liquid Substances 0.000 claims description 20
- 210000004027 cell Anatomy 0.000 claims description 19
- 239000007789 gas Substances 0.000 claims description 15
- 239000006260 foam Substances 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 2
- 238000005485 electric heating Methods 0.000 claims 2
- 229910001220 stainless steel Inorganic materials 0.000 claims 2
- 239000010935 stainless steel Substances 0.000 claims 2
- CFOAUMXQOCBWNJ-UHFFFAOYSA-N [B].[Si] Chemical compound [B].[Si] CFOAUMXQOCBWNJ-UHFFFAOYSA-N 0.000 claims 1
- 210000005229 liver cell Anatomy 0.000 claims 1
- 231100000252 nontoxic Toxicity 0.000 claims 1
- 230000003000 nontoxic effect Effects 0.000 claims 1
- 238000005086 pumping Methods 0.000 claims 1
- 210000004185 liver Anatomy 0.000 abstract description 14
- 210000004369 blood Anatomy 0.000 abstract description 10
- 239000008280 blood Substances 0.000 abstract description 10
- 210000004748 cultured cell Anatomy 0.000 abstract description 9
- 238000002474 experimental method Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 abstract 1
- 239000000306 component Substances 0.000 description 38
- 239000012531 culture fluid Substances 0.000 description 23
- 210000003494 hepatocyte Anatomy 0.000 description 18
- 239000012528 membrane Substances 0.000 description 8
- 210000002381 plasma Anatomy 0.000 description 6
- 229910000831 Steel Inorganic materials 0.000 description 5
- 239000010959 steel Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 239000012510 hollow fiber Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 239000003570 air Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 2
- 235000012489 doughnuts Nutrition 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000005714 functional activity Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000005297 pyrex Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 1
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241001484259 Lacuna Species 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
一种生物反应器，涉及用于临床实验的杂化(或生物)人工肝体外支持系统生物反应器。主要由配气罐[1]、主培养罐[2]和从培养罐[3]和交换柱[4]组成。配气罐[1]与主培养罐[2]之间用硅胶导管相连，并装有蠕动泵[82]；主培养罐[2]与交换柱[4]之间用硅胶导管相连，并装有蠕动泵[84]；硅胶导管嵌入蠕动泵[81]、[82]泵头的管槽内。配气罐[1]与主培养罐[2]之间构成一个培养液的循环。主培养罐[2]与交换柱[4]之间形成另一个培养液的循环。在本实用新型中，患者的血液与被培养细胞没有直接接触。在培养过程中可根据需要增加或更换细胞培养罐。罐体由恒温水浴箱保持恒温。本实用新型生物反应器内细胞培养的环境可由计算机调控。
A bioreactor relates to a bioreactor for a hybrid (or biological) artificial liver support system in vitro used in clinical experiments. It is mainly composed of gas distribution tank [1], main culture tank [2], slave culture tank [3] and exchange column [4]. The gas distribution tank [1] is connected with the main culture tank [2] with a silica gel conduit and equipped with a peristaltic pump [82]; the main culture tank [2] is connected with the exchange column [4] with a silica gel conduit and equipped with a There is a peristaltic pump [84]; the silicone conduit is embedded in the tube grooves of the peristaltic pump [81], [82] pump heads. A circulation of culture solution is formed between the gas distribution tank [1] and the main culture tank [2]. Another circulation of the culture solution is formed between the main culture tank [2] and the exchange column [4]. In the present invention, the patient's blood does not directly contact the cultured cells. During the cultivation process, cell culture tanks can be added or replaced as needed. The tank is kept at a constant temperature by a constant temperature water bath. The environment for cell culture in the bioreactor of the utility model can be regulated by a computer.
Description
Technical field
This utility model relates to a kind of bioreactor, particularly a kind of hydridization (or biological) external back-up system bioreactor of artificial liver that is used for clinical experiment.
Background technology
At present to use more be hollow-fiber bioreactor being used for the external back-up system of the hydridization of clinical experiment (or biological) artificial liver.Its structure and principle are exactly hemodialyzer.Just the selection in membrane material and aperture is different.The doughnut of being made by semipermeable membrane is sealed among the column type container, has so just formed interior exocoel.Respectively among these two cavitys, carried out two-way mass exchange by the blood samples of patients of cultivating hepatocyte and flowing through by semipermeable membrane.
Hollow-fiber bioreactor since the restriction of its structure can to hold cell quantity less, be difficult to satisfy the treatment demand of hydridization (or biological) artificial liver.In incubation because cell distribution is inhomogeneous, reasons such as exchange area finite sum oxygen supply deficiency, cell is deposited on reactor bottom easily and forms agglomerate and not only influence the growth of cell but also be unfavorable for mass exchange.Dead cell can't in time be discharged bioreactor, forms the aggregation of pollutant.
Can't be in existing bioreactor system to being carried out on-line testing by the functional activity of cultured cell.And cultivate hepatocellular state is the effect that can directly have influence on hydridization (or biological) the external support effect of artificial liver.
The utility model content
The purpose of this utility model is the bioreactor that provides for hydridization (or biological) artificial liver and cell culture studies.It has avoided the shortcoming of hollow-fiber bioreactor, is beneficial to hepatocellular High Density Cultivation, and can be according to demand to being expanded by the total amount of cultured cell.For the dead cell that comes off from carrier, rely on isolated plant can discharge bioreactor in time.Control Computer is except carrying out the prosecution the conventional physical quantity of bioreactor, also by with realized cooperating of the online qualitative detection device of cell state being cultivated hepatocellular real-time detection in the culture tank.In case it is unusual to find to be cultivated the active appearance of hepatocyte function, just can remind operator that the operational factor of bioreactor is adjusted in time.Continued to worsen if cultivate hepatocellular state, this bioreactor has guaranteed to change in time, safely from structure is cultivated hepatocyte.So just this link of hepatocyte that can use from bioreactor provides safeguard as the curative effect of the external support of hydridization (biology) artificial liver.
The technical solution adopted in the utility model:
This utility model bioreactor places a bottom to be equipped with in the vertical chassis of deflecting roller.Constant water bath box is placed in the bottom of cabinet, distribution jar, main culture tank and place from culture tank in the temperature chamber of constant water bath box.Peristaltic pump and exchange column are placed in the middle part of cabinet.The cabinet internal upper part is placed Control Computer, is used for system's control.Deforming can is placed in the outer top of cabinet, is used to eliminate the foam that produces in the distribution jar when gas is dissolved in culture fluid.
Between distribution jar and the deforming can, between distribution jar and the main culture tank, main culture tank and from peristaltic pump and silica gel catheter are housed respectively between the culture tank, silica gel catheter embeds in the tube seat of Wriggling Pump Head, distribution jar and deforming can, distribution jar and main culture tank, distribution jar and main culture tank couple together respectively.When placing the peristaltic pump work at cabinet middle part, the rotation of pump head drivewheel compresses the roller of installing on the pump head again and again and loosens the interior silica gel catheter of pump head tube seat, make to produce negative pressure in the pipe, so just liquid draw (gas) body sends in streams to a place in the container of reception from out-put container.
In this utility model, constitute the circulation of a culture fluid between distribution jar and the culture tank, in this circulation, finish regulation and control culture fluid dissolved oxygen and acid-base value.Meanwhile, form the circulation of another culture fluid between culture tank and the exchange column, in this circulation, finish the exchange of culture fluid and blood samples of patients/blood plasma target substance.
On the distribution jar, be fixed with the rustless steel upper end cover, foam outlet joint, air inlet pipe integrated component, drawing liquid interface integrated component and gas-adding pipe integrated component be installed at this upper end cover upper edge circumference.Dissolved oxygen meter integrated component, thermometer boss integrated component are installed, are got dead cell interface integrated component, pH meter interface integrated component, feed liquor interface integrated component at the rustless steel upper end cover upper edge of main culture tank circumference.In main culture tank, lay hanging basket integrated component and following hanging basket integrated component.Place the carrier that is used for being cultivated the hepatocyte attaching in the space between upper and lower hanging basket integrated component, cultivated hepatocyte in this space, to survive.
Cultivated the suitable survival environment of hepatocyte foundation for giving, air, oxygen, carbon dioxide gas and nitrogen dissolve within the culture fluid according to demand under component computer control in the distribution jar.The foam of following collapse of bubbles and producing is admitted to deforming can by the latex pipeline that is connected between distribution jar and the deforming can.In deforming can, the air-flow that sprays in the core jet pipe is pushed foam to ring-like wall net.Gas in the lather collapse under the effect of pressure, foam overflows from removing the flow container top.Liquid after the lather collapse is compiling except that the flow container bottom, and is sent back to the distribution jar under peristaltic pump drives.Acid-base value suits and is full of the enter culture tank of the culture fluid of oxygen molecule in the peristaltic pump driving.
Being cultivated hepatocyte deposits in the main culture tank.And can determine whether needs are also being put into by the cultivation hepatocyte in culture tank according to total cell concentration demand of external support of artificial liver or experiment.When also putting into by the cultivation hepatocyte in culture tank, culture fluid is provided together by the culture fluid circulation line of main culture tank.
Temperature, dissolved oxygen and acidity-basicity sensor are placed within the main culture tank, monitor its variation in real time.The online qualitative detection device of cell state can be drawn quantitative culture fluid and analyze by the three way cock on being connected to culture tank and exchange column being connected silica gel catheter.In time the reflection culture tank is interior by the state of cultured cell, and test result is passed to component computer.Component computer will remind operator that the operational factor of bioreactor is adjusted in time.
Exchange column is selected the dialyser in the conventional blood cleaning equipment for use, and it is made up of hundreds and thousands of porous hollow capillary fiber pipes.Doughnut is and is arranged in parallel, and places in the column type gas-tight silo, forms two independently lacunas inside and outside the fiber pipe semipermeable membrane, i.e. tube cavity and pipe exocoel.When stream respectively by the liquid in inside and outside two chambeies in molecular weight of material during less than the molecular interception amount of semipermeable membrane, just can carry out two-way mass exchange.Contain the inner chamber that is conveyed into exchange column by the culture fluid of cultured cell product under the driving of peristaltic pump from culture tank, patient's blood/plasma is sent into exocoel.The molecular interception amount is at 50-100KD when the semipermeable membrane aperture is selected in 0.2 μ m.Can ensure like this by by the useful active substance of cultured cell synthesis secretion, can add in patient's the body, the biological synthetic functional of performance hydridization (biology) people merit liver as albumin, thrombin, Hepatopoietin and use thereof etc.Help the intravital metabolite of patient, toxicant simultaneously by being transformed and the metabolic detoxification function in culture tank by the picked-up of cultivation hepatocyte, conversion, metabolism, performance hydridization (biology) artificial liver.The macromolecular immune globulin of restriction of choosing in semipermeable membrane aperture sees through, can make on the one hand by the cultivation hepatocyte and avoid the immune influence of patient, can stop the foreign protein of being cultivated the hepatocyte generation to enter the blood samples of patients circulation on the other hand, avoid taking place allergy or rejection.
This utility model uses the thermostatic equipment of constant water bath box as distribution jar and culture tank, and the also available heating wire that has temperature controller is wrapped in the tank body outside and reaches the constant temperature purpose.When using constant water bath box, the tank body of distribution jar and culture tank can be made with disposable plastic that hepatocyte is had no side effect or Pyrex.When selecting for use the heating wire that has temperature controller to be wrapped in the tank body outside when reaching the constant temperature purpose, the tank body that distribution jar and culture tank can only adopt Pyrex to make.
This utility model can make operator in time grasp and be cultivated hepatocellular state.By the operational factor of this utility model bioreactor is adjusted, make by the hepatocellular functional activity of cultivation to be in the purpose that optimum state reaches the external support curative effect of guarantee hydridization (biology) artificial liver.
The beneficial effects of the utility model:
1, adopted the form of dividing jar on the structure, reduced because bubble eruption is cultivated hepatocellular influence to quilt.
2, the application of bicirculating circulation style of culture fluid and exchange column has been avoided being contacted with the direct of blood samples of patients/blood plasma by the cultivation hepatocyte, makes that being cultivated hepatocyte can avoid the immune influence of patient again when can obtaining better oxygen supply and nutrient substance.
3, the dead cell that comes off from carrier can be discharged culture tank in time, has avoided the aggregation of harmful substance in bioreactor.
4, can be adjusted easily as required by the total amount of cultured cell, amplify easily or dwindle.Particularly when patient's liver being carried out have the quilt of replacing to cultivate the hepatocyte demand in the external supporting process, easy to operate.
When this utility model was used for external hydridization (biology) artificial liver, it can bring into play artificial liver support and therapeutical effect better as core component.Particularly have when changing the biomaterial demand when Supporting Therapy's process, this minute the body structure handled easily.This utility model also can be used as breadboard research equipment, carries out cell culture experiments, cell carrier experiment and extracts cellular products.
Description of drawings
Fig. 1 is that system of the present utility model constitutes sketch map; Among the figure: 1 distribution jar; 2 main culture tank; 3 from culture tank; 4 exchange columns; 5 deforming cans; 6 waste liquid tanks; The online qualitative detection device of 7 cell states; 81,82,83,84 peristaltic pumps; 9 gases; 10 foams; 11 culture fluid; 12 blood samples of patients/blood plasma;
Fig. 2 is a distribution jar sketch map; Among the figure: 201. foam outlet joints: 202. feed tube integrated components; 203. liquid suction pipe integrated component; 204. return the liquid interface integrated component; 205. gas-adding pipe integrated component; 206. rustless steel upper end cover;
Fig. 3 is main culture tank sketch map; Among the figure: 301 dissolved oxygen meter interface integrated components; 302 thermometer boss integrated components; 303 get dead cell interface integrated component; 304pH meter interface integrated component; 305 feed liquor interface integrated components; Hanging basket integrated component on 306; 307 times hanging basket integrated components;
Fig. 4 is the cabinet scheme of installation; Among the figure: 401 place the constant water bath box position; 402 install the gas interface position; 403 place the peristaltic pump position; 404 place the exchange column position; 405 place computer location; 406 place the deforming can position; 407 place the waste liquid tank position; 408 folding supporting plates.
The specific embodiment
The utility model is described in further detail below in conjunction with the drawings and specific embodiments.
As shown in Figure 1, distribution jar 1, main culture tank 2, form this utility model bioreactors from culture tank 3 and exchange column 4.This utility model bioreactor places a bottom to be equipped with in the vertical chassis of deflecting roller, as shown in Figure 4.Among Fig. 4, the foot of cabinet is the position 401 of placing constant water bath box.Distribution jar 1, main culture tank 2 and place from culture tank 3 in the temperature chamber of constant water bath box. Peristaltic pump 81,82,83,84 is installed on the middle part of cabinet and places peristaltic pump place 403.Exchange column 4 is installed on the middle part of cabinet and places exchange column place 404.Computer place 405 is placed on the top that computer is placed in the cabinet.Deforming can 5 is placed in the outer top, top of cabinet and places deforming can place 406.
As shown in Figure 1, link to each other with silica gel catheter between distribution jar 1 and the deforming can 5.Deforming can 5 is used to eliminate the foam 10 that produces in the distribution jar when gas 9 is dissolved in culture fluid.After foam 10 broke in deforming can 5, the culture fluid 11 of formation was compiling except that flow container 5 bottoms, and was sent back to the distribution jar under peristaltic pump 81 drives.Link to each other with silica gel catheter between distribution jar 1 and the main culture tank 2, silica gel catheter embeds in the tube seat of peristaltic pump 82 pump heads.Main culture tank 2 with between culture tank 3, link to each other with silica gel catheter.Link to each other with silica gel catheter between main culture tank 2 and the exchange column 4, silica gel catheter embeds in the tube seat of peristaltic pump 84 pump heads.Link to each other with silica gel catheter between waste liquid tank 6 and the main culture tank 2, silica gel catheter embeds in the tube seat of peristaltic pump 83 pump heads.Waste liquid tank 6 is placed in Fig. 4 cabinet lower floor and places waste liquid tank place 407.The online qualitative detection device 7 of cell state is placed on the folding supporting plate 408 at cabinet middle part.
Fig. 2 is distribution jar 1 sketch map.Distribution jar 1 tank body is the hollow cylinder shape glass jar, fixing rustless steel upper end cover 206 on distribution jar 1.Foam outlet joint 201, feed tube integrated component 202, liquid suction pipe integrated component 203 are installed, are returned liquid interface integrated component 204, gas-adding pipe integrated component 205 at this end cap upper edge circumference.
Fig. 3 is the sketch map of main culture tank 2.Main culture tank 2 tank bodies are hollow cylinder shape glass jars, at the fixing rustless steel upper end cover of main culture tank 2 upper port.Pacify dissolved oxygen meter interface integrated component 301, thermometer boss integrated component 302, get dead cell interface integrated component 303, pH meter interface integrated component 304, feed liquor interface integrated component 305 at this upper end cover upper edge circumference.In main culture tank, put into hanging basket integrated component 307 down earlier; And then put into hanging basket integrated component 306.
Operation principle of the present utility model and process such as following.
As shown in Figure 1, constitute the circulation of a culture fluid between distribution jar 1 and the main culture tank 2, in this circulation, finish regulation and control culture fluid dissolved oxygen and acid-base value.Meanwhile, form the circulation of another culture fluid between main culture tank 2 and the exchange column 4.In this circulation, finish the exchange of culture fluid and blood samples of patients/blood plasma target substance.For the oxygen dissolved and the acid-base value of culture fluid in the regulator control system, air, oxygen, carbon dioxide gas and the nitrogen gas 9 after according to certain mixed is sent in the distribution jar 1.The foam of following bubble to break to produce 10 is admitted to deforming can 5 de-bubbles.Acid-base value culture fluid 11 suitable and that be full of oxygen molecule is admitted to culture tank 2 under peristaltic pump drives.Cultivated that hepatocyte is deposited in main culture tank 2 or in culture tank 3.The online qualitative detection device 7 of cell state can be connected three way cock on the silica gel catheter and draws quantitative culture fluid and analyze by being connected to main culture tank 2 with exchange column 4.In time the reflection culture tank is interior by the state of cultured cell, and test result is passed to component computer.
Component computer will be regulated and control the physical environment of bioreactor by actuator according to detected data.Contain a cavity that is conveyed into exchange column 4 by the culture fluid 11 of cultured cell product under the driving of peristaltic pump 84, patient's blood/plasma 12 is sent into the another one cavity.Carry out two-way mass exchange by semipermeable membrane here.The dead cell that comes off from carrier in the culture tank can enter waste liquid tank 6 avoids forming pollutant in culture tank aggregation under the driving of peristaltic pump 81.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420121031 CN2761164Y (en) | 2004-12-30 | 2004-12-30 | Biological reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420121031 CN2761164Y (en) | 2004-12-30 | 2004-12-30 | Biological reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2761164Y true CN2761164Y (en) | 2006-03-01 |
Family
ID=36094299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420121031 Expired - Fee Related CN2761164Y (en) | 2004-12-30 | 2004-12-30 | Biological reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2761164Y (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101837151A (en) * | 2010-03-19 | 2010-09-22 | 南方医科大学珠江医院 | Two-way filling type bioreactor control system and method |
| CN101724557B (en) * | 2009-12-11 | 2012-05-30 | 南开大学 | Polysaccharide composite stent perfusion type liver cell reactor system for use in medicament-induced hepatotoxicity evaluation |
| CN104147652A (en) * | 2014-08-01 | 2014-11-19 | 周平 | Bio-artificial liver system |
| CN104147651A (en) * | 2014-08-01 | 2014-11-19 | 周平 | Hemoperfusion bio-artificial liver system |
| CN105255731A (en) * | 2015-11-02 | 2016-01-20 | 南方医科大学珠江医院 | Circulation filling type cell culture system and bioreactor of circulation filling type cell culture system |
| CN106701542A (en) * | 2017-03-22 | 2017-05-24 | 洛阳师范学院 | Multifunctional culture device for bacteria biofilm |
| CN110106084A (en) * | 2019-05-17 | 2019-08-09 | 西南交通大学 | Cell culture apparatus and the method for making culture medium hydrogen-rich |
| CN110358683A (en) * | 2019-07-25 | 2019-10-22 | 中国科学院电工研究所 | A kind of bioreactor automatic control device |
-
2004
- 2004-12-30 CN CN 200420121031 patent/CN2761164Y/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724557B (en) * | 2009-12-11 | 2012-05-30 | 南开大学 | Polysaccharide composite stent perfusion type liver cell reactor system for use in medicament-induced hepatotoxicity evaluation |
| CN101837151A (en) * | 2010-03-19 | 2010-09-22 | 南方医科大学珠江医院 | Two-way filling type bioreactor control system and method |
| CN101837151B (en) * | 2010-03-19 | 2012-07-04 | 南方医科大学珠江医院 | Two-way filling type bioreactor control system and method |
| CN104147652A (en) * | 2014-08-01 | 2014-11-19 | 周平 | Bio-artificial liver system |
| CN104147651A (en) * | 2014-08-01 | 2014-11-19 | 周平 | Hemoperfusion bio-artificial liver system |
| CN104147652B (en) * | 2014-08-01 | 2017-04-12 | 武汉仝干医疗科技股份有限公司 | Bio-artificial liver system |
| CN105255731A (en) * | 2015-11-02 | 2016-01-20 | 南方医科大学珠江医院 | Circulation filling type cell culture system and bioreactor of circulation filling type cell culture system |
| CN106701542A (en) * | 2017-03-22 | 2017-05-24 | 洛阳师范学院 | Multifunctional culture device for bacteria biofilm |
| CN110106084A (en) * | 2019-05-17 | 2019-08-09 | 西南交通大学 | Cell culture apparatus and the method for making culture medium hydrogen-rich |
| CN110358683A (en) * | 2019-07-25 | 2019-10-22 | 中国科学院电工研究所 | A kind of bioreactor automatic control device |
| CN110358683B (en) * | 2019-07-25 | 2022-04-26 | 中国科学院电工研究所 | A bioreactor automatic control device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107904169B (en) | Modularized bioreactor suitable for culturing multiple cell types | |
| WO2019120135A1 (en) | Cell culture module and cell culture system | |
| CN102296029B (en) | Perfusion Bioreactor System | |
| CN201626959U (en) | Microperfusion Devices for Cell Culture | |
| CN104893974B (en) | Can online dynamic realtime monitoring portable expansion of stem cells culture device | |
| CN109055216A (en) | High-throughput 3D cell, class loading and organoid dynamic cultivation system | |
| JPH05184351A (en) | Cell culture apparatus | |
| JP2004510431A (en) | Automated bioculture and bioculture experiment system | |
| US20060033222A1 (en) | Devices for introducing a gas into a liquid and methods of using the same | |
| JP2001504692A (en) | Cell culture incubator | |
| WO2011090781A1 (en) | A single use cell culture bioreactor manifold system | |
| CN2761164Y (en) | Biological reactor | |
| CN101732770B (en) | Double-cavity liquid storage tank for biological artificial liver support system | |
| CN1840651A (en) | Safe and efficient continuous closed cell culture virus production/inactivation system | |
| CN106520552A (en) | Cell culture biological reactor | |
| CN1257262C (en) | Perfusion bioreactor system constructed in vitro with bilayer active skin | |
| WO2019120136A1 (en) | Cell culture module, culture solution module, cell culture chip, and cell culture case | |
| CN102783414A (en) | External circulation mist-type bioreactor for large-scale cultivation of plant hairy roots | |
| CN103131635A (en) | Cavity-type dynamic-filling bioreaction device | |
| CN107641594B (en) | Differential pressure type open pipeline bioreactor for microalgae culture | |
| CN102373151B (en) | Bioreactor and photo bio culture systems | |
| CN202626198U (en) | High-throughput cell culture apparatus easy for cell wall removal | |
| CN100497582C (en) | Bioreactor system for nerve stem cell to amplify in large scale and long time | |
| CN202543220U (en) | High-throughput cell culture incubator | |
| CN203280815U (en) | Non-bubble oxygen supply type bioartificial liver cell culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |