CN2751028Y - Suspended biochip - Google Patents
Suspended biochip Download PDFInfo
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- CN2751028Y CN2751028Y CN 200420045343 CN200420045343U CN2751028Y CN 2751028 Y CN2751028 Y CN 2751028Y CN 200420045343 CN200420045343 CN 200420045343 CN 200420045343 U CN200420045343 U CN 200420045343U CN 2751028 Y CN2751028 Y CN 2751028Y
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- biochip
- microballoon
- suspension
- microsphere
- probe
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Abstract
The utility model relates to a suspended biochip which comprises a polymer microsphere served as a carrier, a suspending medium for maintaining the suspended state of the microsphere, bioprobes and a container for accommodating a biochip, wherein various microspheres covered with bioprobes are mixed in the suspending medium according to certain scale, microspheres of different sizes are served as carriers of different bioprobes, and the size of the microsphere corresponds to the kind of the bioprobe. With the utility model, when a reading apparatus reads the suspended biochip, the kind of the bioprobe, or the antigen and antibody can be distinguished only by detecting the size of the microsphere. Compared with the method of detecting the marked color of the microsphere, the utility model has the advantages of simple and reliable operation, low requirement to the equipment and reduced machining and detecting cost.
Description
Technical field
The utility model relates to the biological technical field of a new generation, relates in particular to a kind of novel suspension biochip.
Background technology
Biochip is used for the detection of biological sample, this technology is born in the beginning of the eighties in last century, be integrated with thousands of the informational molecules relevant with life by micro fabrication in chip, it can carry out integrated to the various biochemical reaction process in life science and the medical science.Be compared to traditional biochemistry detection technology, its advantage is can test simultaneously in theory and analyze several even several thousand, several ten thousand kinds of different Biological indicators.The gene diagnosis and the fields such as antigen, antibody test that are widely used in gene sequencing, detection in Gene Mutation and polymorphism analysis, genomic library pattern analysis, disease.Be considered to new millennium one of the most promising biological technical field.
Biochip is divided into microarray formula and floated (claiming streaming again) two kinds by the carrier difference.Microarray formula biochip, briefly on the silicon chip that poly-lysine bag quilt is arranged of a thumbnail, probe biomolecule is arranged with the form of large scale array exactly, formation can interact with molecules of interest, the solid phase surface of Bank of Communications's reaction, exciting down mark fluorescent to present different fluorescence emission spectrums respectively according to the real reaction situation in the order of laser levies, CCD camera or laser confocal microscope are according to its wavelength and wave amplitude feature collection signal, Computer Analysis data results.What the most successful isocyanides formed certain commercial market at present is the DNA chip, is about to countless pre-designed oligonucleotide or cDNA and makes dot matrix on chip, with homologous nucleic acid molecular hybridization [3] in the sample.Allow the biomolecules of almost all kinds to make probe on the chip in fact in theory, such as albumen, sugar, lipid and other small molecules.Can measure simultaneously in theory and reach several ten thousand indexs, but the preparation more complicated of chip, the preparation cost height, the labeling process of sample is loaded down with trivial details, and signal sensitivity is low, the poor repeatability of test.Make this technology can only be used for scientific research, be difficult in clinical diagnosis, popularize.
The suspension biochip technology is called streaming technology again, is the significant improvement to microarray technology.In the prior art, its technical characterstic is: use the carrier of the microballoon of different colours as different bioprobes, the color of microballoon and the kind of bioprobe are corresponding one by one, microballoon is done the Two Colour Fluorescence mark of varying strength, make microballoon line up a line on the track of determining by liquid flow field during detection, order is passed through detecting area.Two restraint the laser cross-fires at detecting area, a branch of marker color that is used for surveying microballoon, and another bundle is used for measuring the fluorescence intensity of composition to be measured.This technology is compared with microarray formula technology, and advantage is to be easy to produce in enormous quantities, and measuring repeatability is good, and is highly sensitive.Shortcoming is that microballoon preparation is complicated, and reaction system is complicated, and background will be through repeatedly washing, and operation is difficult for, and simultaneously, is used to survey the equipment requirements height of the color of microballoon.
Summary of the invention
In order to overcome the deficiency of existing biochip and reading technique, a purpose of the present utility model is: a kind of novel suspension biochip is provided, not only is easy to produce in enormous quantities, measuring repeatability is good, and is highly sensitive, and the microballoon preparation process obtains simplifying.
The technical scheme that its technical problem that solves the utility model adopts is:
A kind of suspension biochip, comprise the polymer microsphere as carrier, the container that makes microballoon keep the suspension medium of suspended state, bioprobe and be used for containing described biochip, the described various microballoon that is coated with probe mixes in suspension medium by a certain percentage, described polymer microsphere has multiple, and the diameter rank of multiple polymer microsphere has nothing in common with each other; Described bioprobe has one or more, and other polymer microsphere bag of diameter class of the same race is by specific a kind of bioprobe.
The beneficial effects of the utility model are, suspension biochip since the microballoon that adopts different sizes as the carrier of different bioprobes or antigen, antibody, the kind of the size of microballoon and probe or antigen, antibody is corresponding one by one, make reading apparatus when reading, only can pick out the kind of probe or antigen, antibody by the size of surveying microballoon, method with respect to the marker color of surveying microballoon, not only simple and reliable, and the requirement of equipment greatly lowered, thereby also lowered processing and detected cost.Method by abundant diluted mixture liquid has replaced the method for background through repeatedly washing, and makes simple to operateization equally, has improved detection efficiency simultaneously.Therefore, can be widely used in the blood station to blood test, hospital makes a definite diagnosis patient and other various biological technical fields.Use the analyzing biochips human genome, can find out cancer, diabetes and cause the morbific gene of disease, will greatly improve the health of the people and health level by genetic defective.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is that No. 1 microballoon bag of the utility model suspension biochip is by reagent A synoptic diagram;
Fig. 2 is that No. 2 microballoon bags of the utility model suspension biochip are by reagent B synoptic diagram;
Fig. 3 is that No. 3 microballoon bags of the utility model suspension biochip are by reagent C synoptic diagram;
Fig. 4 is the synoptic diagram of the utility model suspension biochip.
The explanation of reference numeral:
The 21-1 microballoon; The 22-2 microballoon; The 23-3 microballoon; The 24-suspension medium.
Embodiment
As shown in Figure 1, 2, 3, be 1 ~ No. 3 microballoon bag by the reagent synoptic diagram, microballoon 21,22,23 diameters are different, for example: microballoon 21 diameter phi D1, microballoon 22 diameter phi D2, microballoon 23 diameter phi D3.The microballoon of different diameter wraps respectively by different types of probe, for example: microballoon 21 bags are by the A probe, and microballoon 22 bags are by the B probe; Probe microballoon 23 bags are by the C probe.Carry out classification by the microballoon that existing different sizes are mixed, can obtain the microballoon of different stage, each its diameter of other microballoon of level is in certain scope.Therefore, as long as the rank of microballoon has determined that the kind of the rank of microballoon and probe or antigen, antibody just can be corresponding one by one.
Preferably, described microsphere diameter size is in 2 ~ 300 micrometer ranges.
Described probe can be DNA, RNA, antigen, antibody, acceptor, part equal samples, for example: germs such as AIDS, gonorrhoea, hepatitis B, hepatitis A.
As shown in Figure 4, be the disclosed a kind of suspension biochip of the utility model, comprise polymer microsphere 21 ~ 23 as carrier, make microballoon keep the suspension medium 24 of suspended state, bioprobe or antigen, antibody and the container that is used for containing described biochip, described various probe or the antigen of being coated with, the microballoon of antibody mixes in suspension medium by a certain percentage, utility model point of the present utility model is, the microballoon that adopts different sizes is as different bioprobes or antigen, the carrier of antibody, the size of microballoon and probe or antigen, the kind of antibody is corresponding one by one.
In the described suspension biochip, can add the microballoon that is coated with different probe or antigen, antibody, also can be as required, only add the microballoon that is coated with probe of the same race or antigen, antibody.
Claims (5)
1, a kind of suspension biochip, comprise the polymer microsphere as carrier, the container that makes microballoon keep the suspension medium of suspended state, bioprobe and be used for containing described biochip, the described various microballoon that is coated with probe mixes in suspension medium by a certain percentage, it is characterized in that: described polymer microsphere has multiple, and the diameter rank of multiple polymer microsphere has nothing in common with each other; Described bioprobe has one or more, and other polymer microsphere bag of diameter class of the same race is by specific a kind of bioprobe.
2, suspension biochip according to claim 1 is characterized in that: described probe is DNA, RNA, antigen, antibody, acceptor, part equal samples.
3, suspension biochip according to claim 1 and 2 is characterized in that: in the described suspension biochip, described microballoon is coated with different types of probe.
4, suspension biochip according to claim 1 and 2 is characterized in that: in the described suspension biochip, described microballoon is coated with the probe of identical type.
5, suspension biochip according to claim 1 and 2 is characterized in that: described microsphere diameter size is in 2～300 micrometer ranges.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420045343 CN2751028Y (en) | 2004-04-29 | 2004-04-29 | Suspended biochip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420045343 CN2751028Y (en) | 2004-04-29 | 2004-04-29 | Suspended biochip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2751028Y true CN2751028Y (en) | 2006-01-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420045343 Expired - Fee Related CN2751028Y (en) | 2004-04-29 | 2004-04-29 | Suspended biochip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2751028Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645067A (en) * | 2016-12-15 | 2017-05-10 | 天津岳达科技有限公司 | Liquid biochip analyzer |
-
2004
- 2004-04-29 CN CN 200420045343 patent/CN2751028Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645067A (en) * | 2016-12-15 | 2017-05-10 | 天津岳达科技有限公司 | Liquid biochip analyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |