CN2634480Y - Solid phase support plate strip for preparing solidified emzyme combined matter - Google Patents
Solid phase support plate strip for preparing solidified emzyme combined matter Download PDFInfo
- Publication number
- CN2634480Y CN2634480Y CN 03250076 CN03250076U CN2634480Y CN 2634480 Y CN2634480 Y CN 2634480Y CN 03250076 CN03250076 CN 03250076 CN 03250076 U CN03250076 U CN 03250076U CN 2634480 Y CN2634480 Y CN 2634480Y
- Authority
- CN
- China
- Prior art keywords
- solid phase
- enzyme
- lath
- antigen
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000007790 solid phase Substances 0.000 title claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 27
- 108090000790 Enzymes Proteins 0.000 claims abstract description 27
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000000427 antigen Substances 0.000 abstract description 12
- 102000036639 antigens Human genes 0.000 abstract description 8
- 108091007433 antigens Proteins 0.000 abstract description 7
- 239000007791 liquid phase Substances 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 2
- 241001122786 Stylidium Species 0.000 abstract 1
- 238000012864 cross contamination Methods 0.000 abstract 1
- 239000000155 melt Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000010276 construction Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920005549 butyl rubber Polymers 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002631 hypothermal effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
Images
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- Enzymes And Modification Thereof (AREA)
Abstract
The utility model is a solid phase supporting lath used in solidified enzyme bonder preparation. The solid phase supporting lath is a porous lath comprising a plurality of small stylidiums (1), and the lath can also be formed by the small stylidums and the existing eight-hole, twelve-hole or sixteen-hole enzyme labeled lath. The lower part of the small stylidum is a through hole (2), and the upper part is a concave hole (3) separated from the lower hole. The solid phase supporting lath used in solidified enzyme bonder preparation of the utility model is applied; when the solidified enzyme labeled antigen or antibody bonder is put into the enzyme labeled lath of 8 holes per strip, 12 holes per strip or 16 holes per strip which is provided with detecting liquid phase samples, solidified enzyme antibody (antigen) bonder melts quickly and combines with solid phase antibody-antigen complexes specificity; the solid phase antibody (antigen)-antigen (antibody)-enzyme labeled antibody (antigen) complexes are generated; and then the enzyme substrate is added to develop. At that time the enzyme quantity on the solid phase carrier positively correlates with the quantity of the detected matter in the sample. The method not only simplifies operation, but also reduces operating error and cross contamination.
Description
Technical field
The utility model relates to the medication preparation apparatus, specifically is used to solidify the solid phase support lath of enzyme conjugates preparation.
Background technology
When doing enzyme linked immunosorbent assay, prior art all adopts the liquid phase enzyme conjugates, operating personnel can use micropipet or quantitative drop bottle adding to add in the micropore of sample, operating personnel's workload is big, complex operation, since operating personnel when squeezing drop bottle angle and the shake of hand all can cause added drop inaccurate, can cause the error and the cross pollution of application of sample, influence the accuracy of measurement result.The liquid phase enzyme conjugates requires to preserve 2～8 ℃ of conditions, and storage life generally is no more than 6 months; For the stability that makes enzyme conjugates improves a lot, the liquid phase enzyme conjugates adopts hypothermic lyophilization technology, be prepared into the enzyme conjugates of curing after, under equal condition, the enzyme conjugates of freeze-drying can be preserved non-inactivation more than a year.But when the freeze-drying prods of preparation liquid phase enzyme, lack a kind of solid support apparatus.
Summary of the invention
The purpose of this utility model provides a kind of required when the freeze-drying prods of preparation liquid phase enzyme, solid phase easy to use, that avoid polluting and supports lath.
The technical solution of the utility model is: the solid phase that is used to solidify the enzyme conjugates preparation supports the structure of lath to be: forms the porous lath by several paxillas, or the ELIAS strip of the 8 holes/bar of this cylinder and use in the market, 12 holes/bar, 16 holes/bar is used.The bottom is a through hole in this paxilla, and top is the shrinkage pool that cuts off with following hole.
Solid phase of the present utility model supports lath to can be made into 8/bar, 12/bar, 16/bar etc., or is used with the ELIAS strip of the 8 holes/bar that uses in the market, 12 holes/bar, 16 holes/bar.The ELIAS strip of solid support and 8 holes/bar, 12 holes/bar, 16 holes/bar can be used, it is the solid support of enzyme conjugates, can enzyme conjugates be assigned in each sample well uniformly, the lid of the enzyme mark micropore of available again and 8 holes/bar, 12 holes/bar, 16 holes/bar can be avoided the cross pollution between each sample well.
Use the solid phase support lath that is used to solidify the enzyme conjugates preparation of the present utility model, when the enzyme labelled antibody that will solidify or antigen bond are put into the ELIAS strip of 8 holes/bar of adding liquid phase sample to be measured, 12 holes/bar, 16 holes/bar, enzyme labelled antibody (antigen) bond that solidifies dissolves rapidly, combine with solid matrix antibody-antigen compound specificity, generate antibody (antigen)-antigen (antibody)-enzyme labelled antibody (antigen) compound of solid phase, add the zymolyte colour developing then; The amount positive correlation of examined object matter in enzyme amount that have on the solid phase carrier this moment and the sample; This method has promptly been simplified operation, can reduce operate miss and cross pollution again.
Description of drawings
Fig. 1 is a battened construction upward view of the present utility model;
Fig. 2 is a battened construction front view of the present utility model;
Fig. 3 is a battened construction vertical view of the present utility model.
Embodiment
Embodiment:
As shown in the figure, solid phase of the present utility model supports lath to form the porous lath by several paxillas 1, or the ELIAS strip of the 8 holes/bar of this paxilla and use in the market, 12 holes/bar, 16 holes/bar is used.The bottom is a through hole 2 in this paxilla, and top is the shrinkage pool 3 that cuts off with following hole.This solid phase supports that the lath selection requires certain degree of hardness to be arranged, as non-toxic plastic, butyl rubber and silica gel etc. for nontoxic, harmless.
Claims (1)
1, a kind of solid phase support lath that is used to solidify the enzyme conjugates preparation, it is characterized in that forming the porous lath by several paxillas (1), or this paxilla cooperates formation with the ELIAS strip of existing 8 holes/bar, 12 holes/bar, 16 holes/bar, the bottom is through hole (2) in this paxilla, and top is the shrinkage pool (3) that cuts off with following hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03250076 CN2634480Y (en) | 2003-08-21 | 2003-08-21 | Solid phase support plate strip for preparing solidified emzyme combined matter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03250076 CN2634480Y (en) | 2003-08-21 | 2003-08-21 | Solid phase support plate strip for preparing solidified emzyme combined matter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2634480Y true CN2634480Y (en) | 2004-08-18 |
Family
ID=34293779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03250076 Expired - Fee Related CN2634480Y (en) | 2003-08-21 | 2003-08-21 | Solid phase support plate strip for preparing solidified emzyme combined matter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2634480Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108139393A (en) * | 2016-09-19 | 2018-06-08 | 生物银行股份公司 | Perform biochemical analysis and the multi-function device and its inspection method of immune response inspection |
-
2003
- 2003-08-21 CN CN 03250076 patent/CN2634480Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108139393A (en) * | 2016-09-19 | 2018-06-08 | 生物银行股份公司 | Perform biochemical analysis and the multi-function device and its inspection method of immune response inspection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |