CN2522423Y - Multi-pot gas-phase double dynamic solid-state fermentation apparatus - Google Patents
Multi-pot gas-phase double dynamic solid-state fermentation apparatus Download PDFInfo
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Abstract
本实用新型涉及的多罐体气相双动态固态发酵装置，包括2或多个卧式圆筒形罐体，每个罐体的前、后端均设有快开门结构，罐体上罐壁两端分别安装由进气阀控制的进气管，下罐壁两端分别安装由排气阀控制的排气管，罐体之间以管道连成一体以形成循环风道，在罐壁内部的上、下、左、右壁附近设有带有各自的冷凝水进口管和冷凝水出水管的冷却排管，最后一个罐体后部罐壁上连通用以强制罐内气体循环的离心式鼓风机；罐内设有轴向放置的罐内固定轨道，罐内固定轨道上安装带浅盘的活动式料盘架，可完成微生物纯种培养，容易放大，发酵效价高，无三废，适用生物农药、酶制剂、农用抗生素、单细胞蛋白等发酵生产。
The utility model relates to a multi-tank gas-phase dual-dynamic solid-state fermentation device, which includes 2 or more horizontal cylindrical tank bodies, each tank body is equipped with a quick-opening door structure at the front and rear ends, and the upper tank wall of the tank body has two The air inlet pipes controlled by the air inlet valves are respectively installed at both ends of the lower tank wall, and the exhaust pipes controlled by the exhaust valves are respectively installed at both ends of the lower tank wall. There are cooling pipes with respective condensate inlet pipes and condensate outlet pipes near the lower, left and right walls, and a centrifugal blower used to force gas circulation in the tank is connected to the tank wall at the rear of the last tank; The tank is equipped with an axially placed fixed track in the tank, and a movable tray rack with a shallow plate is installed on the fixed track in the tank, which can complete pure microbial culture, easy to enlarge, high fermentation titer, no three wastes, suitable for biological pesticides , enzyme preparations, agricultural antibiotics, single-cell protein and other fermentation production.
Description
Technical field
The utility model belongs to the fermentation industry technical field, particularly a kind of multiple tank body gas-phase double-dynamic solid fermentation device.
Technical background
Cooperated with chemical engineer by the microbiologist since 1945, the penicillin that Fu Laiming is found has successfully been pushed to since the suitability for industrialized production, and the liquid submerged fermentation technology has opened whole modern fermentation industry.Solid state fermentation lacks the engineering solution because of problems such as conveying, stirring, temperature, humidity, pH, oxygen supplys, does not reach the requirement of modern fermentation industry and out in the cold always.Key issue is not meet modern fermentation industry to require sophisticated solid-state fermenter.This has become a global problem.
Modern fermentation industry makes the liquid submerged fermentation technology occupy dominant position to the strict demand of purebred cultivation of microorganism and scale operation.Solid-state fermentation process and equipment are because of its easy microbiological contamination, and fermentation condition is restive, and reasons such as difficulty are amplified in industry, are considered to a kind of ancient and backward fermentation process always.But the plurality of advantages of solid state fermentation is brief as technical process, and raw material sources are extensive, cheap, and energy consumption is low, and is pollution-free etc., in the bio-reactor development, has powerful magnetism and vitality.This backward situation of solid state fermentation industry for a change since half a century, since especially the new subject of the seventies biochemical engineering proposes, has been no lack of the someone and has attempted to break through this road difficult problem, proposes multiple solution.B.K.Lonsanc reduces nine types with it: (1) rotary drum, and (2) tub formula, (3) add a cover disc type, and (4) vertically cultivate boxlike, and (5) tilt to inoculate boxlike, (6) tray formula, (7) conveyor type, (8) column type, (9) are hybrid.K.E.Aidou is divided into ten types with it, and is similar.If divide, then can be generalized into static and dynamic two big classes by the culture medium movement state.Static state is meant that substratum does not move, and makes mass transfer, heat transfer, oxygen supply difficulty; Temperature, humidity, pH are difficult to control.Being meant dynamically that substratum is in is interrupted or the movement continuously state, though mass transfer, heat transfer, oxygen supply have improvement greatly, mechanical part is unfavorable to aseptic technique, and material stirs the energy consumption height, destroys mycelium, and engineering is amplified difficulty etc.
People make great efforts to improve as koji tray, bent case, Quchi on the one hand to the conventional solid-state fermentor tank, mainly are to make strong physical work mechanize, strict ventilation condition; Actively develop the closed type mechanical stirring solid state fermentation device that is suitable for the purebred cultivation requirement of microorganism on the other hand.Wherein studying maximum is rotary drum, also has Multi-layer rotary top or rotary rake type, and it is tower to turn over root after many, conveyor type, mechanical lifting formula etc.All because of the transmission rig complexity, the strict seal difficulty, the dead angle such as is difficult for cleaning up at reason, still difficultly reaches the actual requirement that modern fermentation industry is amplified purebred cultivation and industry.
The used solid-state fermenter of existing production still is traditional tray formula and thick-layer ventilation pond formula device, also has directly substratum to be pressed into round pie or the square brick piece places in the room.The method that scale is bigger is the continuous shallow tray fermentation of tunnel kiln, is static types, can only be used for the spontaneous fermentation production process.From the theoretical angle of chemical reaction engineering " three biographies one are anti-", be reinforcing mass transfer, heat transfer, dynamically fermentation container should be a developing direction.Therefore the dynamic fermentation reactor of closed once was the domestic and international research focus.Especially the rotary drum solid-state fermentation reactor has a large amount of relevant research reports, and enters into the commerical test stage.All because of being difficult to prevent that living contaminants is unable to bear the long-term production test.The airtight dynamic fermentation reactor of other type all is confined to the laboratory exploratory stage.
Summary of the invention
The purpose of this utility model is to solve the problem of easily dying assorted bacterium, heat and mass and amplification difficulty that present solid-state fermentation installation exists, and provides the purebred cultivation of a kind of energy, transfer rate is fast and multiple tank body gas-phase double-dynamic solid fermentation device that easily amplify.
Embodiment of the present utility model is as follows:
The multiple tank body gas-phase double-dynamic solid fermentation device that the utility model provides, it is characterized in that: comprise 2 or a plurality of horizontal circle tube tank body 1, before each tank body 1, the rear end is equipped with and can opens fast, close jar quick opening structure 6 of door, the inlet pipe by intake valve 10 controls is installed at the last tank skin two ends of each tank body 1 respectively, the vapor pipe by vent valve 11 controls is installed at the following tank skin two ends of each tank body 1 respectively, fuse to form circulation air path with pipeline between the tank body, on tank skin inside, down, a left side, respectively be provided with cooling coil 8 near the right wall, each cooling coil 8 is provided with water of condensation inlet pipe and water of condensation rising pipe separately, is communicated with on last tank body 1 rear portion tank skin in order to force jar centrifugal blower 9 of interior gas circulation; Be provided with the jar internal fixing track 16 of axial placement in each tank body 1, on the jar internal fixing track 16 the movable charging tray frame 14 of making rolling movement thereon is installed, the confession that movable charging tray frame 14 is provided with a plurality of horizontal positioned holds the tray 15 of solid medium, and the movable charging tray frame 14 of each tank body 1 is a row or two rows; Described fast-open door panel structure 6 is the side set structure: be respectively equipped with on tank body front end and the cover facing surfaces and stagger mutually and close-fitting concavity side set 61 and the convex side set 611 that is concaveconvex shape, cover is carried out the bayonet-type locking by motor drive and tank body front end; Each tank body be provided with outward be positioned at tank body 1 within jar outer movable rail 17 that links to each other of axial jar internal fixing track 16, the other end of the outer movable rail 17 of a jar jar external fixation track 18 outer with being arranged on tank body is connected, and is arranged on the movable charging tray frame 14 turnover solid-state fermenters of mechanical haulage car 19 tractions on jar external fixation track 18.
The multiple tank body gas-phase double-dynamic solid fermentation device that the utility model provides is solid substances to be fermented to be inserted pressure pulsation and circulate in two dynamic environment of air to carry out solid state fermentation; Described pressure pulsation environment is: inject sterile air by the air inlet solenoid control that is contained on the fermentor tank tank body in the fermentor tank tank body, make its pressure-controlling at 1.5-3.5Kg/cm
2In the scope, when pressure reaches this set(ting)value, close the air inlet magnetic valve, keep this pressure 1-5min simultaneously, open the exhaust solenoid valve that is contained on the fermentor tank tank body then, pressure release in the tank body, keeping a jar internal pressure after the pressure release is 0.1-0.5Kg/cm
2, close exhaust solenoid valve, dimension is pressed 10-60 second; Afterwards, open the air inlet magnetic valve once more, repeat above-mentioned pressurization and pressure leak process, realize that just fermentation materials is in pulsating pressure and circulate solid state fermentation under the gas-phase double attitude control of air.
Basic thought of the present utility model is that the gaseous pressure people in the solid-state fermenter is pulsed for imposing the cycle, and the cycle of pulsation, amplitude and waveform will change with different fermentations object, fermentation time; Secondly, in the gas-phase double-dynamic solid fermentation jar, establish circulation, air channel and many group cooling coils, force jar interior gas phase air in turn by material and cooling coil, the heat in the material is taken away in pressure, and forced hot air and spiral coil cooling tube thermal exchange, reduce the temperature of fermentation materials, and guaranteed the uniformity and the controllability of leavening temperature and humidity.This gas-phase double attitude method and apparatus will cause triple effect: (1) quickens the metabolism of microorganism; (2) transmission of acceleration nutrition and meta-bolites outer room in cell walls; (3) the interior intersubjective heat and mass transport mode of gas phase of gas phase in the space and reactor will be transformed into the convection current diffusion by molecular diffusion between solid medium accumulation horizon endoparticle.
Microbial metabolism produces in the traditional solid-state heap fermentation, solid culture medium accumulation horizon heat and carbonic acid gas distribute, and mainly are to adopt thin layer, and machinery stirs or the bottom air-blasting method solves.And the thin layer fermentation is by shortening the stroke of static molecular diffusion in the space, reaching the heat radiation purpose.Machinery stirs or the bottom air blast is that variation is diffused as the convection current diffusion, but thalline is had very macrolesion.Adopt the operation of gas-phase double attitude also can be diffused as the convection current diffusion by variation, when gas phase main body increased pressure, gas can flow into the space, until reaching pressure equilibrium.When gas phase main body pressure reduced, the gaseous pressure in the space also can make pressure reduce thereupon flowing into the gas phase main body, reaches low-pressure balance; Simultaneously, force jar interior gas phase air, guaranteed the uniformity and the controllability of leavening temperature and humidity in turn by material and cooling coil.
For example, adopt the work of gas-phase double attitude fermentation products obtained therefrom enzyme alive higher 2～3 times than constant voltage products obtained therefrom enzyme to cellulase fermentations.Fermentation time is general to shorten 1/3rd.For example B.t fermentation is 3 days during constant voltage, is 2 days during transformation.To muscardine or green muscardine fungus, be generally 7 days, be 5 days during transformation.Riboflavin fermentation can foreshorten to 7 days from 12 days, or the like.The spore count alive of fermentation of white muscardine fungi, experiment can reach 80,000,000,000/gram, generally 50,000,000,000/gram more than.
The utlity model has following characteristics:
1. do not have solid layer machinery and stir device, reach the heat and mass transport requirement by thin layer, gas-phase double attitude and recirculation blower.
2. because no solid stirs mechanical transmission, the simple sealing easily of structure of reactor is convenient to industry and is amplified.
3. reactor is a pressure-vessel, and available pressure steam carries out strict slack tank or real jar of sterilization.Cleaning is convenient at no dead angle.
4. adopt the aseptic compressed air oxygen supply, reactor is a barotropic state in the fermenting process, so can strictness reach purebred solid-state culture requirement.
5. the gas-phase double attitude has the promotion microbial metabolism, strengthens the inside and outside mass transfer of cell, reduces the feedback inhibition of meta-bolites, thereby the function that shortens fermentation period, raising transformation efficiency is arranged.
6. the ring structure of reactor and recycle blower make temperature, humidity uniformity in the reactor.
7. in the reactor cooling coil is set, after recirculation blower cooperates, forces jar interior gas phase air in addition, reduce the temperature of fermentation materials, and be convenient to the regulation and control of reactor temperature and humidity in turn by material and cooling coil.
8. the cycle of gas-phase double attitude, amplitude and waveform are finished by the in good time automatic control system of air valve, can require to carry out the control of computer on-line optimization to oxygen supply and heating with fermenting process.
The essential distinction of the utility model and conventional solid-state fermentation has: (1) has realized proper purebred cultivation.Realized the scale suitability for industrialized production.(2) period stimulating makes the fermentation toxicity evaluation improve 3-5 doubly.(3) temperature in the fermenting process, humidity, pH are controlled.(4) the vacuum condensation drying does not only make toxicity evaluation reduce, and increases on the contrary. (5) three-waste free discharge.(6) though facility investment than conventional solid-state fermentation method height, but more much lower than liquid submerged fermentation.
This process for solid state fermentation thoroughly changes the popular idea of " solid fermentation is a kind of ancient and backward production method ".Existing fermentation engineering and biochemical engineering textbook will be rewritten, and it is more advanced than liquid submerged fermentation method in all many-sides.The modern fermentation industry history of technique spiral step of having passed by.
This gas-phase double-dynamic solid fermentation method has extensive applicability and dissemination, is a kind of novel process for solid state fermentation that is different from liquid submerged fermentation method.Realized that modern fermentation engineering is desired: purebred cultivation on the technology, the double goal of extensive industrialization on the engineering.This new and high technology also has initiative in the world, because it also is the world-famous puzzle of generally acknowledging in the world for a long time.
The research that the applicant adhered to solid-state fermentation technology and fermentation unit from 1984 always, 1999 be under the principle of design of " extraneous period stimulating enhanced biological reaction and the inside and outside rate of mass transfer of cell " of propulsion source, develop gas-phase double-dynamic solid fermentation method and apparatus of the present utility model in " four pass one anti-" nonlinear theory and with the normal force; And be tested to 800 liters of pilot scales from 0.5 liter, again to 25000 liters and 70000 liters of technical scales; Build up the exemplary factory of the extensive purebred cultivation Bacillus thuringiensis wettable powder of a solid state fermentation, demonstrated many economic targets that is better than the liquid submerged fermentation technology; Being the breakthrough to China's Bacillus thuringiensis study on the industrialization, also is the breakthrough to solid-state fermentation technology, also might be the breakthrough to " biological respinse engineering " new subject basic theory.
Schedule of operation is as follows: it is aseptic at first with steam slack tank (comprising movable charging tray frame) to be carried out sterilising treatment, cooling then, feed sterile air simultaneously, open quick operating valve fast by quick opening structure in during aseptic technique, pull out movable charging tray frame, solid state fermentation hypothallus after uniform distribution on the multilayer tray inserts bacterial classification, push in the tank body of fermentation unit at once, close quick operating valve, adjust temperature and humidity in the tank body, impose the control of gas-phase double attitude, can utilize heating system to implement the vacuum condensation drying after fermentation finishes.● working method
Main operation is to impose periodically pulsing with sterile air to pressing in the jar, and the change curve of tank pressure as shown in Figure 1; The pressurising of tank body gaseous pressure by sterile air with rush down pressure, cyclic swing between peak pressure and pneumatics; Cycle time is by the pressurising time, peak pressure steady time rushes down pressure time and pneumatics four sections compositions steady time, is in barotropic state in the tank body all the time.The peak pressure value is generally 1.5--3.5kg/cm
2, pneumatics is generally 0.1--0.5kg/cm
2, the pressurising time is longer, and curve rises mild, generally at 1～5 minute, is decided by the source of the gas ability; Rush down and press time requirement short as far as possible, generally within one minute, especially began for ten seconds, pressure drop rate is fast, makes the gas between the moist particle of solid medium expansion suddenly take place and the bed of material is taken place loosening; Peak pressure time and pneumatics time are controlled by artificial setting, and general peak pressure steady time is greater than pneumatics steady time, and change with fermentation time, generally at increased logarithmic phase, the change frequency height, lag period and stationary phase frequency little.Be generally cycle time 15 minutes to 150 minutes.Because tank pressure is simple to operate, control easily automatically.
Description of drawings
Accompanying drawing 1 is a structural representation of the present utility model;
Accompanying drawing 2 is that rectangle of the present utility model is every the longitudinal cross-section of tube synoptic diagram;
Accompanying drawing 3 be jar internal fixing track with a jar outer movable rail 17, jar external fixation track 18 between structural representation;
Accompanying drawing 4 is a tank body front end side set structural representation;
Accompanying drawing 5 is the side set structural representation of cover;
Wherein, Tank body 1 quick opening structure 6 intake valves 10
Water of condensation inlet pipe 811 water of condensation water inlet pipes 812 cooling coils 8
Movable charging tray frame 14 trays 15 centrifugal blowers 9
17 jars of external fixation tracks of the outer movable rail of jar, 18 mechanical haulage cars 19
611 jars of internal fixing tracks 16 of concavity side set 61 convex side sets
Embodiment
Further describe the utility model below in conjunction with drawings and Examples:
Embodiment 1: produce B.t biological pesticide wettable powder with solid-state fermentation installation solid state fermentation shown in Figure 1:
As shown in Figure 1, gas-phase double-dynamic solid fermentation device of the present utility model, comprise 2 or a plurality of horizontal circle tube tank body 1, before each tank body 1, the rear end is equipped with and can opens fast, close jar quick opening structure 6 of door, the inlet pipe by intake valve 10 controls is installed at the last tank skin two ends of each tank body 1 respectively, the vapor pipe by vent valve 11 controls is installed at the following tank skin two ends of each tank body 1 respectively, fuse to form circulation air path with pipeline between the tank body, on tank skin inside, down, a left side, respectively be provided with cooling coil 8 near the right wall, each cooling coil 8 is provided with water of condensation inlet pipe and water of condensation rising pipe separately, is communicated with on last tank body 1 rear portion tank skin in order to force jar centrifugal blower 9 of interior gas circulation; Be provided with the jar internal fixing track 16 of axial placement in each tank body 1, on the jar internal fixing track 16 the movable charging tray frame 14 of making rolling movement thereon is installed, the confession that movable charging tray frame 14 is provided with a plurality of horizontal positioned holds the tray 15 of solid medium, and the movable charging tray frame 14 of each tank body 1 is a row or two rows; Described fast-open door panel structure 6 is the side set structure: be respectively equipped with on tank body front end and the cover facing surfaces and stagger mutually and close-fitting concavity side set 61 and the convex side set 611 that is concaveconvex shape, cover is carried out the bayonet-type locking by motor drive and tank body front end; Each tank body be provided with outward be positioned at tank body 1 within jar outer movable rail 17 that links to each other of axial jar internal fixing track 16, the other end of the outer movable rail 17 of a jar jar external fixation track 18 outer with being arranged on tank body is connected, and is arranged on the movable charging tray frame 14 turnover solid-state fermenters of mechanical haulage car 19 tractions on jar external fixation track 18.
The bacterial classification that present embodiment uses: Bacillus thuringiensis (B.t Var Kurstaki); Fermented substrate: wheat bran, cotton seed powder cake, Semen Maydis powder, rice bran, corn steep liquor, unslaked lime, moisture 51-60%; Fermentating controling condition: 30 ℃ of temperature, humidity 95%, pressure cycle 10-60 minute, amplitude 0.5-3.0Kg/cm
2, waveform: quick pressure releasing and pressurising.
With gas-phase double-dynamic solid fermentation device of the present utility model, triangular flask fermentation unit and porcelain dish fermentation unit above-mentioned material is carried out the solid state fermentation simultaneous test:
Its concrete fermentation step is: solid substances that will be to be fermented is inserted the pressure pulsation of present embodiment and is circulated in the gas-phase double-dynamic solid fermentation device of air (two dynamic environment) and carries out solid state fermentation; In the fermentor tank tank body, inject sterile air by the air inlet solenoid control, make its pressure-controlling at 1.5-3.5Kg/cm
2In the scope, when pressure reaches this set(ting)value, close the air inlet magnetic valve, keep this pressure 1-5min simultaneously, open the exhaust solenoid valve that is contained on the fermentor tank tank body then, pressure release in the tank body, keeping a jar internal pressure after the pressure release is 0.1-0.5Kg/cm
2, close exhaust solenoid valve, dimension is pressed 10-60 second; Afterwards, open the air inlet magnetic valve once more, repeat above-mentioned pressurization and pressure leak process, realize that just fermentation materials is in pulsating pressure and circulate solid state fermentation under the gas-phase double attitude control of air.
Triangular flask fermentation unit and porcelain dish fermentation unit are traditional fermentation unit, are not given unnecessary details at this;
Bacillus thuringiensis Bacillus thuringiensis (B.t) sterilant is generally acknowledge in the world the most effective, also the most representative microbial ecological preparation, belong to the biological control category, be used to kill the alkaline stomach insects of 10 orders kind more than 500 such as lepidopteran, Diptera, Coleoptera, Orthoptera, store vegetables, fruit tree, cotton, tealeaves, tobacco, forest, corn, paddy rice, soybean, grain, and even fields such as mosquito, fly all have good pest control effect.Because it only has toxic action to alkaline stomach insect, thus harmless to the people, animal and the birds of acid stomach, environmentally safe, insect is difficult to form resistance, has been the most important microbial pesticide of emergence rapidly in the world since the eighties.
The World Health Organization examines already, and B.t is to the person poultry harmless, and is harmless to poultry, birds, fish.Never find both at home and abroad that in decades the B.t producers had intoxicating phenomenon.
China B.t quality ministerial standard has two series products, totally six specifications.A kind of is emulsion, and toxicity evaluation has 2000IU/ μ l, 4000IU/ μ l, 8000IU/ μ l Three Estate.Another kind is a wettable powder, and toxicity evaluation has 8000IU/mg, 16000IU/mg, 32000IU/mg.The wettable powder of world market is generally 16000IU/mg.Home products mostly is emulsion, toxicity evaluation 2000IU/ μ l.
Use gas-phase double-dynamic solid fermentation device of the present utility model, continuous 55 jars batches, average toxicity evaluation 16000IU/mg, the highest batch reaches 23000IU/mg.Compare with existing liquid submerged fermentation route, gas-phase double-dynamic solid fermentation is produced B.t biological pesticide wettable powder feasibility study conclusion.
1, the B.t wettable powder is that national environmental protection is badly in need of product, and pollution-free food production is acquired a special sense.The issue circular order of since last year national each province and city government, vegetables produce and ban use of chemical pesticide, for the B.t development provides new opportunity.
2, the gas-phase double-dynamic solid fermentation device is that China develops voluntarily, all has great initiative meaning at home and abroad.Compare with current both at home and abroad liquid submerged fermentation technology, facility investment has only 1/4th, and production cost has only 1/2.5th.For solid foundation has been established in the establishment and the development of the Ecological Industrial and the ecological agriculture.
3, China's liquid submerged fermentation method production B.t wettable powder technology does not reach a standard as yet, generally sells with emulsion, and preservation period has only three months, and the wettable powder preservation period can be more than 3 years.This solid-state fermentation installation will be started the new situation of China B.t wettable powder industrialization.
Its solid state fermentation comparative test result such as following table:
| The fermentation container type | Fermentation time (hour) | Spore count alive (hundred million/gram) | Toxicity evaluation (IU/mg) | Crystal separation rate (%) |
| The utility model solid-state fermenter | 42--48 | 250--400 | 12000--20000 | ＞80 |
| Triangular flask (contrast) | 72 | 100--150 | 4000--6000 | 30--50 |
| Porcelain dish (contrast) | 72 | 150--200 | 6000--8000 | 50 |
Embodiment 2: what present embodiment used is three tank structures of the present utility model, and other steps are with embodiment 1, the result such as the following table of the solid state fermentation simultaneous test of its " microorganism feed addictive with cured shape genus bacillus (Bacillus cereus) DM423 ":
| Fermentation process | Type of reactor | Fermentation time (hour) | (hundred million/gram (milliliter) of spore counts alive | Cost (unit/ton) |
| Liquid submerged fermentation | The standard mechanical jar | 48 | Hundred million/milliliter of 30--50 | 15000 |
| Solid state fermentation | The utility model solid-state fermenter | 48 | Hundred million/gram of 200--300 | 3000 |
| Porcelain dish | 48 | Hundred million/gram of 80--200 | Microbiological contamination is arranged |
Embodiment 3: device that present embodiment uses and step be with embodiment 1, to carry out the result of solid state fermentation of " production of muscardine biological pesticide " as follows:
Muscardine is a kind of entomogenous fungi of imperfect fungi, and host range is wide, and virulence is strong, to the toxicological harmless of people and animals forest crop, does not injure natural enemy, and is free from environmental pollution, is one of most widely used microbial pesticide.Beauveria is in the fungal pathogens of insect, and epidermis or pore by insect enter in the insect body, and insect is caused a disease and dead.Leafeating insect all there is insecticidal effect preferably, particularly injurious forest-insect is had special efficacy.Market outlook are wide.At present, China is first big country that produces and use beauvericin insecticide, uses about 500,000 hectares of area every year, is making significant contribution aspect control pine moth and the Pyrausta nubilalis (Hubern)..Abroad, generally adopt liquid fermenting to produce muscardine, its production cost height; Domestic is that tray is cultivated mostly, and industrial scale is little, and is seriously polluted, and the product instability is that seasonal on a small scale production the in indivedual forest farms used by oneself.China produces the muscardine preparation and mainly adopts solid-state cultivation of tray and liquid-solid two-phase method.Problems such as this law also exists complex process, the production cycle is long, cost is high, unstable product quality, its product also can not satisfy market demand far away.
The period stimulating solid-state fermenter that adopts us to develop is for many years produced muscardine, can guarantee pure-blood ferment, and the fermentation of easily amplification scale, and the potency ratio tray is cultivated and improved 2-3 doubly.The spore count alive of fermentation of white muscardine fungi, experiment can reach 80,000,000,000/gram, generally 50,000,000,000/gram more than.
Claims (4)
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| CN 02201134 CN2522423Y (en) | 2002-01-22 | 2002-01-22 | Multi-pot gas-phase double dynamic solid-state fermentation apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488301A (en) * | 2011-11-23 | 2012-06-13 | 中国水产科学研究院渔业机械仪器研究所 | Tandem compound type solid state fermentation device |
| CN104876660A (en) * | 2015-04-23 | 2015-09-02 | 浙江海洋学院 | Sludge composting system |
| EP3805358A1 (en) * | 2019-10-08 | 2021-04-14 | Green Spot Technologies SAS | Process and production system for large scale solid-state fermentation |
-
2002
- 2002-01-22 CN CN 02201134 patent/CN2522423Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488301A (en) * | 2011-11-23 | 2012-06-13 | 中国水产科学研究院渔业机械仪器研究所 | Tandem compound type solid state fermentation device |
| CN102488301B (en) * | 2011-11-23 | 2013-08-14 | 中国水产科学研究院渔业机械仪器研究所 | Tandem compound type solid state fermentation device |
| CN104876660A (en) * | 2015-04-23 | 2015-09-02 | 浙江海洋学院 | Sludge composting system |
| CN104876660B (en) * | 2015-04-23 | 2017-09-05 | 浙江海洋学院 | Sludge Composting System |
| EP3805358A1 (en) * | 2019-10-08 | 2021-04-14 | Green Spot Technologies SAS | Process and production system for large scale solid-state fermentation |
| WO2021069538A3 (en) * | 2019-10-08 | 2021-06-24 | Green Spot Technologies | Process and production system for large scale solid-state fermentation |
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