CN2472816Y

CN2472816Y - Multiple sectioned centrifugal tube

Info

Publication number: CN2472816Y
Application number: CN 01212825
Authority: CN
Inventors: 张建国; 皇甫留杰; 付坚
Original assignee: ZHONGMIN GENE ENGINEERING Co LTD WUHAN
Current assignee: Shenzhen Sanjiu Gene Engineering Co., Ltd.
Priority date: 2001-03-22
Filing date: 2001-03-22
Publication date: 2002-01-23
Anticipated expiration: 2011-03-22

Abstract

The utility model discloses a multiple sectioned centrifugal tube, which consists of a tube body, a tube cover, a sealing board and a protective film. A partition running through the tube cavity is arranged in the tube cavity of the common centrifugal tube body to divide the tube cavity into a plurality of non-communicating sections and divides the tube cavity into 2 to 6 sections, thereby making one tube with multiple functions and equivalent conditions. Consequently, multiple groups of PCR amplification reactions can be done against the same background in the same tube, and the tube is also applicable to the preservation, delivery and reaction of the micro-metering multilateral system under the same conditions. Furthermore, one tube with multiple functions improves the efficiency, simplifies the amplification process and prevents pollution effectively.

Description

Multi-division centrifugal tube

The utility model belongs to the reaction centrifuge tube in a kind of bioengineering gene field, is provided with the centrifuge tube of separation in especially a kind of tube chamber.

In the prior art, subregion not in the employed common centrifuge tube tube chamber in the genetic engineering field, can only put into one group of sample in the every common centrifuge tube reacts or/and separate, many group samples must be put into a plurality of centrifuge tubes and react or/and separate, this is for the test of little metering of the molecular level in the gene field, be easy to generate especially and pollute and pollution mutual interference mutually mutually, and, when requiring many group samples under same background, to finish, for example many group PCR (Polymerase chain reaction, when polymerase chain reaction) primer amplification reacts in a plurality of centrifuge tubes, its temperature, time, experimental conditions etc. all are difficult to control in full accordly.

The purpose of this utility model is at the limitation of common centrifuge tube in the prior art and deficiency, and the centrifuge tube that is provided with separation in a kind of tube chamber is provided, make many group biochemical reactions can be in equality of temperature, simultaneously, finish with under the condition, and will pollute may be reduced to minimum.

In order to solve above-mentioned task; the utility model is to realize like this; it mainly is made up of body and pipe lid; in the tube chamber of common centrifuge tube body, be provided with the partition that tube chamber is divided into the perforation tube chamber in several mutual disconnected zones; sealing plate is round pie; be placed on the partition on body top; the diaphragm that be thin discs shape consistent with the sealing plate diameter is placed on the sealing plate; the lower edge of outer that body is suitable for reading and pipe cover cavity is provided with screw thread or the elastic annular bayonet socket band that cooperatively interacts, and the top of pipe cover cavity is provided with the boss that can compress sealing plate.

Subregion is 2～6 in the tube chamber; have the corresponding to circular hole of number of regions and position that several and tube chamber are separated on the sealing plate; the diameter of sealing plate is consistent with body internal diameter suitable for reading; the thickness of sealing plate is more than or equal to the edge suitable for reading of body and the difference in height between the partition, and diaphragm and sealing plate can adopt hot pressing or adhesive bond.

Body, Guan Gai, sealing plate are made by the transparent polypropylene of white, and diaphragm is made by the transparent polyethylene from high pressure process of white, also can make of transparent, high pressure resistant, free of contamination other material.

The utility model simplicity of design has solved the limitation of common centrifuge tube dexterously, makes one tube multipurpose, the condition equalization, can be with finishing many group pcr amplification reactions under the background in pipe, to multiple system can be in equality of temperature, simultaneously, finish biochemical reaction with under the condition, reduce multiple reacting phase mutual interference.The setting of sealing plate plays and concentrates the effective antipollution effect of sealing, is applicable to that also little metering multi-primers is with preservation under the condition, transportation and pcr amplification.One tube multipurpose has improved efficient, easy amplification technology, and saved a large amount of common centrifuge tubes.

Below in conjunction with accompanying drawing the specific embodiment of the present utility model is described in further detail:

Accompanying drawing 1 is a multi-division centrifugal tube assembling schematic diagram;

Accompanying drawing 2 is triple division centrifugal tube body schematic diagrames;

Accompanying drawing 3 is multi-division centrifugal tube pipe lid schematic diagrames;

Accompanying drawing 4 is sealing plate schematic diagrames.

Figure acceptance of the bid keep the score state as follows: 1---body; 2---the pipe lid; 3---sealing plate; 4---diaphragm;

5---cut off; 6---boss; 7---circular hole.

In accompanying drawing 1～4; body 1 covers 2 with pipe and is combined by screw thread; cut off 5 inner chambers and be separated into a plurality of mutual disconnected zones body 1; it is lower slightly than the upper edge of body 1 up and down to cut off 5 perforation tube chambers; sealing plate 3 diameters are consistent with body internal diameter suitable for reading; thickness is consistent with difference in height between partition and the body upper edge; when using the utility model; after placing the multiple reaction mixture of little metering (comprising the PCR reaction system) in a plurality of zones respectively; sealing plate 3 is pressed into body top to be placed on the partition 5; diaphragm 4 diameters are consistent with the diameter of sealing plate 3, are positioned on the sealing plate lid upper tube cap 2; threaded together; the boss that pipe covers 2 inner chamber tops compresses sealing plate under the control of threaded adjusting structure, and after reaction was finished, the rifle point of will sampling stabbed out diaphragm 4 respectively and enters body inner each district sampling respectively.

Claims

1; a kind of multi-division centrifugal tube; mainly by body; the pipe lid is formed; it is characterized in that: in the tube chamber of common centrifuge tube body, be provided with the partition (5) that tube chamber is divided into the perforation tube chamber in several mutual disconnected zones; sealing plate (3) is round pie; be placed on the partition on body (1) top; the diaphragm that be thin discs shape (4) consistent with the sealing plate diameter is placed on the sealing plate; the lower edge of outer that body (1) is suitable for reading and Guan Gai (2) inner chamber is provided with screw thread or the elastic annular bayonet socket band that cooperatively interacts, and the top of pipe lid (2) inner chamber is provided with the boss (6) that can compress sealing plate.

2, multi-division centrifugal tube according to claim 1; it is characterized in that: subregion is 2～6 in the tube chamber; have the corresponding to circular hole of number of regions and position (7) that several and tube chamber are separated on the sealing plate (3); the diameter of sealing plate (3) is consistent with body (1) internal diameter suitable for reading; the thickness of sealing plate is more than or equal to the edge suitable for reading of body and the difference in height between the partition, and diaphragm and sealing plate can adopt hot pressing or adhesive bond.

3, multi-division centrifugal tube according to claim 1 and 2; it is characterized in that: body (1), pipe lid (2), sealing plate (3) are made by the transparent polypropylene of white; diaphragm (4) is made by the transparent polyethylene from high pressure process of white, also can make of transparent, high pressure resistant, free of contamination other material.