CN222564934U - Synchronous separation system for cow milk protein components - Google Patents
Synchronous separation system for cow milk protein components Download PDFInfo
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- CN222564934U CN222564934U CN202420634123.XU CN202420634123U CN222564934U CN 222564934 U CN222564934 U CN 222564934U CN 202420634123 U CN202420634123 U CN 202420634123U CN 222564934 U CN222564934 U CN 222564934U
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Abstract
The utility model relates to the technical field of separation and purification of cow milk proteins, and provides a cow milk protein component synchronous separation system which comprises a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line. The membrane filtration and the chromatographic technique are combined, and the separation of different active proteins is realized by utilizing the molecular weight, the charged property, the hydrophobic property and the like of the proteins. The utility model can separate the main active proteins in the milk, the beta-casein, the alpha-lactalbumin, the immune globulin and the lactoferrin are sequentially separated and purified from the raw milk, and are dried or aseptically filled, different products are prepared while the natural nutrition components are maintained, and the separated milk can still be used for processing into sterilized milk or milk beverage.
Description
Technical Field
The utility model relates to the technical field of separation and purification of cow milk proteins, in particular to a synchronous separation system of cow milk protein components.
Background
Milk proteins are also known as cow milk proteins, and are a general term for a mixture of many proteins in milk. Mainly comprises casein and whey protein. Whey protein is water-soluble protein and consists of alpha-lactalbumin, beta-lactoglobulin and other components. Alpha-lactalbumin contains a large number of amino acids such as leucine and isoleucine which are essential to the human body but cannot be synthesized by the human body. The casein comprises alpha-casein, beta-casein, kappa-casein and other components, and has physiological effects on decayed tooth, preventing and treating osteoporosis and rickets, regulating blood pressure, treating iron deficiency anemia, magnesium deficiency neuritis and other diseases. However, in the related art, the separation method of cow milk proteins is generally aimed at separating a certain component of cow milk separately, and does not systematically realize the separation of various active proteins in cow milk.
Disclosure of utility model
The utility model provides a synchronous separation system for cow milk protein components, which is used for solving or improving the problem that the simultaneous separation of a plurality of active proteins in milk cannot be realized in the prior art.
The utility model provides a milk protein component synchronous separation system which comprises a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line, wherein the first cation exchange device is connected with the milk bin;
The milk bin, the fat separator and the first cation exchange device are sequentially connected, a first outlet of the first cation exchange device is connected with the first membrane filtration module, a first outlet of the first membrane filtration module is connected with the casein solution treatment branch, a second outlet of the first membrane filtration module is connected with the second membrane filtration module, a first outlet of the second membrane filtration module is connected with the second cation exchange device, a first outlet of the second cation exchange device is connected with the first anion exchange device, and a second outlet of the second cation exchange device is connected with the second anion exchange device;
The second outlet of the first cation exchange device, the second outlet of the second membrane filtration module, the first anion exchange device and the second anion exchange device are all connected with the purification and sterilization treatment branch, and the purification and sterilization treatment branch is connected with the product production line.
According to the synchronous separation system for the milk protein components, which is provided by the utility model, the synchronous separation system for the milk protein components further comprises a heat exchanger and a first material temporary storage tank, wherein a first outlet of the first cation exchange device is connected with the first membrane filtration module through the heat exchanger and the first material temporary storage tank.
According to the synchronous separation system for the milk protein components, which is provided by the utility model, the casein solution treatment branch comprises a second material temporary storage tank, a material melting device, a UHT sterilization machine and a filling machine which are sequentially connected, and a first outlet of the first membrane filtration module is connected with the second material temporary storage tank.
According to the synchronous separation system of the cow milk protein components, which is provided by the utility model, the synchronous separation system of the cow milk protein components further comprises a third material temporary storage tank, the second outlet of the first membrane filtration module is connected with the second membrane filtration module through the third material temporary storage tank, and/or,
And the second outlet of the second membrane filtration module is connected with the purification and sterilization treatment branch through the fourth material temporary storage tank.
According to the synchronous separation system for the cow milk protein components, which is provided by the utility model, the synchronous separation system for the cow milk protein components further comprises a fifth material temporary storage tank, and the first outlet of the second membrane filtration module is connected with the second cation exchange device through the fifth material temporary storage tank.
According to the synchronous separation system for the cow milk protein components, which is provided by the utility model, the synchronous separation system for the cow milk protein components further comprises a sixth material temporary storage tank and a seventh material temporary storage tank, wherein the first outlet of the second cation exchange device is connected with the first anion exchange device through the sixth material temporary storage tank, and the first anion exchange device is connected with the purification and sterilization treatment branch through the seventh material temporary storage tank.
According to the synchronous separation system for the cow milk protein components, which is provided by the utility model, the synchronous separation system for the cow milk protein components further comprises an eighth material temporary storage tank and a ninth material temporary storage tank, wherein the second outlet of the second cation exchange device is connected with the second anion exchange device through the eighth material temporary storage tank, and the second anion exchange device is connected with the purification and sterilization treatment branch through the ninth material temporary storage tank.
According to the synchronous separation system for the cow milk protein components, which is provided by the utility model, the synchronous separation system for the cow milk protein components further comprises a tenth material temporary storage tank, and the second outlet of the first cation exchange device is connected with the purification and sterilization treatment branch through the tenth material temporary storage tank.
According to the cow milk protein component synchronous separation system provided by the utility model, the purification and sterilization treatment branch comprises a concentration and purification device and a sterilization filter which are sequentially connected.
According to the milk protein component synchronous separation system provided by the utility model, the product production line comprises a freeze drying branch, a spray drying branch and a filling branch which are connected in parallel.
Compared with a single process for separating single active protein, the synchronous separation system for the cow milk protein components provided by the utility model combines membrane filtration with chromatographic technology, and realizes separation of different active proteins by utilizing the molecular weight, charged property, hydrophobic property and the like of the proteins. That is, in this embodiment, the main active proteins in milk can be separated, and β -casein, α -lactalbumin, immunoglobulin, lactoferrin are sequentially separated and purified from raw milk, and dried or aseptically filled, so that different products can be prepared while natural nutrients are maintained, and the separated milk can still be used for processing into sterilized milk or milk beverage.
Drawings
In order to more clearly illustrate the utility model or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a synchronous separation system for components of milk proteins.
Reference numerals:
1. Milk bin, 2 milk cleaning machine, 3 fat separator, 4 first cation exchanger, 5 heat exchanger, 6 first material temporary storage tank, 7 first membrane filtration module, 8 second material temporary storage tank, 9 material melting device, 10 UHT sterilization machine, 11 filling machine, 12 third material temporary storage tank, 13 second membrane filtration module, 14 fourth material temporary storage tank, 15 fifth material temporary storage tank, 16 second cation exchanger, 17 sixth material temporary storage tank, 18 first anion exchanger, 19 seventh material temporary storage tank, 20 eighth material temporary storage tank, 21 second anion exchanger, 22 ninth material temporary storage tank, 23 tenth material temporary storage tank, 24 purification and sterilization treatment branch, 25 product production line.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In recent years, with the improvement of living standard, development of functional foods using bioactive substances as natural materials has been attracting more attention. Cow milk is considered to be the best source of natural active substances, and active proteins in cow milk include lactoferrin, alpha-lactalbumin, beta-lactoglobulin, immunoglobulins, and the like.
Currently, these milk active proteins are not well utilized by humans. Most of the milk is UHT milk, and most of active substance components are damaged and lose the original efficacy due to high heating strength of the UHT milk. For example, the lactoferrin component in the raw milk is 120ml/L, the component in the finished product after processing is 0, the loss rate is 100%, the alpha lactalbumin and beta milk globule components in the raw milk are 1000mg/L and 3000mg/L respectively, the loss rate in the finished product after processing is 100mg/L and 300mg/L respectively, and the loss rate is more than 90%.
How to avoid heat loss of these milk proteins is a current problem faced by the dairy industry. With the popularization and application of membrane filtration and chromatographic separation technology in dairy products, various active proteins can be separated and purified. At present, whey protein in milk is separated by using a membrane filtration technology to prepare high-overflow-price whey protein powder, and the lactoferrin of cow milk is extracted by using a chromatographic separation technology and added into a normal-temperature product in a sterile adding mode. These methods are generally directed to certain components of cow's milk and do not systematically effect the separation of various active proteins in milk.
In order to solve the above problems, as shown in fig. 1, in an alternative embodiment, the synchronous separation system for cow's milk protein components of the embodiment of the present utility model comprises a milk bin 1, a fat separator 3, a first cation exchange device 4, a first membrane filtration module 7, a casein solution treatment branch, a second membrane filtration module 13, a second cation exchange device 16, a first anion exchange device 18, a second anion exchange device 21, a purification and sterilization treatment branch 24, and a product production line 25.
Milk bin 1, fat separator 3 and first cation exchange device 4 are connected in order, still can set up clean milk machine 2 between milk bin 1 and fat separator 3, the first export of first cation exchange device 4 is connected with first membrane filtration module 7, the first export of first membrane filtration module 7 is connected with casein solution treatment branch road, the second export of first membrane filtration module 7 is connected with second membrane filtration module 13, the first export of second membrane filtration module 13 is connected with second cation exchange device 16, the first export of second cation exchange device 16 is connected with first anion exchange device 18, the second export of second cation exchange device 16 is connected with second anion exchange device 21.
Wherein the second outlet of the first cation exchange device 4, the second outlet of the second membrane filtration module 13, the first anion exchange device 18 and the second anion exchange device 21 are all connected with a purification and sterilization treatment branch 24, and the purification and sterilization treatment branch 24 is connected with a product production line 25.
Fresh milk is collected and stored in the milk silo 1. According to actual needs, a stirrer and a cooling device can be arranged in the milk bin 1 so as to ensure the freshness and uniformity of milk. Subsequently, milk is pumped out of the milk bin 1 and is pretreated by the milk purifier 2 so as to remove larger particle impurities and insoluble substances in the milk, thereby ensuring the smooth proceeding of the subsequent treatment process. The pretreated milk then enters the fat separator 3. At this stage, the fat separator 3 separates the fat portion of the milk from the skim milk by physical centrifugation. Skim milk contains higher protein content and is the main raw material for separating protein components later.
Specifically, raw milk in the milk bin 1 enters the milk purifier 2 for pretreatment through a centrifugal pump and a flowmeter, the pretreated milk enters the fat separator 3 to become skim milk, and the skim milk enters the first cation exchange device 4 through the centrifugal pump and the flowmeter. After passing through the first cation exchange device 4, the lactoferrin in the milk is adsorbed on a chromatographic column, a chromatographic elution process is carried out to obtain a lactoferrin solution, the lactoferrin solution enters a product production line 25 through a purification and sterilization treatment branch 24, milk without the lactoferrin flows through the chromatographic column (flow through liquid) and then enters the first membrane filtration module 7, and the milk is divided into two parts through the first membrane filtration module 7, wherein one part is a trapped liquid casein solution, the other part is a permeate whey protein solution, and the whey protein solution is rich in beta-casein components. The casein solution enters a casein solution treatment branch and is used for preparing pure milk or milk beverage. The whey protein solution enters the second membrane filtration module 13. The second membrane filtration module 13 can separate the beta-casein component from the whey protein, the trapped liquid is beta-casein solution, the permeate is whey protein solution, the beta-casein solution enters the product production line 25 through the purification and sterilization treatment branch 24, the whey protein solution enters the second cation exchange device 16 through the pump and the flowmeter, the content of the protein is detected in real time through the ultraviolet protein sensor, the immunoglobulin in the whey protein is adsorbed on the cation chromatographic column, the alpha-lactalbumin and the beta-lactalbumin flow through, the alpha-lactalbumin and the beta-lactalbumin solution enter the first anion exchange device 18, the alpha-lactalbumin is adsorbed on the chromatographic column, and the beta-lactalbumin flows through. The eluted alpha-lactalbumin solution enters a product production line 25 through a purification and sterilization treatment branch 24, and the immunoglobulin solution enters a second anion exchange device 21, wherein the immunoglobulin is adsorbed on a chromatographic column, and other miscellaneous proteins flow through. The eluted immunoglobulin solution passes through purification and sterilization treatment branch 24 to product production line 25. Wherein the first anion exchange means 18 and the second anion exchange means 21 differ in the filler.
In the embodiment of the utility model, compared with the single process for separating single active protein, the membrane filtration and chromatographic technique are combined, and different active proteins are separated by utilizing the molecular weight, the charged property, the hydrophobic property and the like of the protein. That is, in this embodiment, the main active proteins in milk can be separated, and β -casein, α -lactalbumin, immunoglobulin, lactoferrin are sequentially separated and purified from raw milk, and dried or aseptically filled, so that different products can be prepared while natural nutrients are maintained, and the separated milk can still be used for processing into sterilized milk or milk beverage.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system of the milk protein components further comprises a heat exchanger 5 and a first temporary material storage tank 6, and the first outlet of the first cation exchange device 4 is connected with the first membrane filtration module 7 through the heat exchanger 5 and the first temporary material storage tank 6.
After the milk without lactoferrin flows through the chromatographic column (flow-through liquid), the milk is cooled to below 8 ℃ through the heat exchanger 5 and enters the first material temporary storage tank 6, the storage time in the first material temporary storage tank 6 is 24 hours, beta-casein in the milk can be dissociated from casein micelles after 24 hours of storage, and the material in the first material temporary storage tank 6 can be pumped to the first membrane filtration module 7.
In an alternative embodiment, as shown in fig. 1, the casein solution processing branch comprises a second material temporary storage tank 8, a melting device 9, a UHT sterilization machine 10 and a filling machine 11, which are connected in sequence, and a first outlet of the first membrane filtration module 7 is connected with the second material temporary storage tank 8.
Among them, the UHT sterilizer 10, that is, the ultra-high temperature sterilizer, is an apparatus for performing a continuous sterilization process of milk, juice beverage or the like. The working principle is that the product is heated to ultra-high temperature (usually 135-150 ℃) for a few seconds, so that bacteria are killed rapidly, and the sterilization effect is achieved.
The casein solution is stored in the second temporary storage tank 8, and the casein solution is used for processing pure milk or milk beverage. Specifically, the casein solution passes through a melting device 9, a UHT sterilization machine 10 and a filling machine 11 in sequence, and finally is aseptically canned into milk or milk beverage finished products.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system of the components of the milk protein further comprises a third material temporary storage tank 12, and the second outlet of the first membrane filtration module 7 is connected with the second membrane filtration module 13 through the third material temporary storage tank 12. The first membrane filtration module 7 may be MF membrane filtration with a pore size of 0.12 μm, and the second membrane filtration module 13 may be UF membrane filtration with a pore size of 20KDa. That is, the whey protein solution enters the third material holding tank 12 for storage.
In addition, the synchronous separation system of the cow milk protein components further comprises a fourth material temporary storage tank 14, and the second outlet of the second membrane filtration module 13 is connected with the purification and sterilization treatment branch 24 through the fourth material temporary storage tank 14. Wherein the beta-casein solution is fed into a fourth material holding tank 14 for storage.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system for bovine milk protein components further comprises a fifth temporary material storage tank 15, and the first outlet of the second membrane filtration module 13 is connected to the second cation exchange device 16 through the fifth temporary material storage tank 15.
The whey protein solution was stored in the fifth material temporary storage tank 15, and then was fed to the second cation exchange apparatus 16 via a pump and a flow meter. Wherein, the protein content is detected in real time by an ultraviolet protein sensor.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system for bovine milk protein components further comprises a sixth temporary material storage tank 17 and a seventh temporary material storage tank 19, wherein the first outlet of the second cation exchange device 16 is connected to the first anion exchange device 18 through the sixth temporary material storage tank 17, and the first anion exchange device 18 is connected to the purification and sterilization treatment branch 24 through the seventh temporary material storage tank 19.
After the whey protein solution enters the second cation exchange device 16, the α -lactalbumin and β -lactoglobulin flow through and are stored in the sixth temporary storage tank 17, and the α -lactalbumin and β -lactoglobulin solution in the sixth temporary storage tank 17 is pumped to the first anion exchange device 18, where the α -lactalbumin is adsorbed on the chromatographic column, and the β -lactoglobulin flows through. The eluted alpha-lactalbumin solution is stored in a seventh material temporary storage tank 19.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system for bovine milk protein components further comprises an eighth temporary material storage tank 20 and a ninth temporary material storage tank 22, wherein the second outlet of the second cation exchange device 16 is connected to the second anion exchange device 21 through the eighth temporary material storage tank 20, and the second anion exchange device 21 is connected to the purification and sterilization treatment branch 24 through the ninth temporary material storage tank 22.
After the whey protein solution enters the second cation exchange device 16, the immunoglobulin in the whey protein will be adsorbed on the cation chromatographic column, the eluted immunoglobulin is stored in the eighth temporary storage tank 20, the immunoglobulin solution stored in the eighth temporary storage tank 20 is pumped to the second anion exchange device 21, the immunoglobulin will be adsorbed on the chromatographic column, and other impurity proteins will flow through, and the eluted immunoglobulin is stored in the ninth temporary storage tank 22.
In an alternative embodiment, as shown in fig. 1, the synchronous separation system of the bovine milk protein components further comprises a tenth material temporary storage tank 23, and the second outlet of the first cation exchange apparatus 4 is connected with the purification and sterilization treatment branch 24 through the tenth material temporary storage tank 23.
It should be noted that the lactoferrin solution is stored in the tenth material temporary storage tank 23, and the lactoferrin solution in the tenth material temporary storage tank 23 may be pumped to the purification and sterilization treatment branch 24.
In an alternative embodiment, as shown in FIG. 1, the purification and sterilization process bypass 24 comprises a concentration and purification device and a sterilization filter connected in series.
It should be noted that the purification and sterilization treatment branch 24 can remove salts in each solution and filter bacteria in the solution to achieve commercial sterility of the solution
In an alternative embodiment, as shown in fig. 1, the product production line 25 includes a freeze drying leg, a spray drying leg, and a filling leg connected in parallel.
The beta-casein solution, alpha-lactalbumin solution, immunoglobulin solution and lactoferrin solution obtained from top to bottom respectively enter a product production line 25 through a purification and sterilization treatment branch 24, and the purification and sterilization treatment branch 24 can remove salt in each solution and filter bacteria in the solution so as to enable the solution to reach a commercial sterile state. The product production line 25 can be frozen into powder by a freeze drying device, can be sprayed into powder by a spray drying device, and can be made into liquid sterile package by a sterile filling machine.
It should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present utility model, and not for limiting the same, and although the present utility model has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the technical solution described in the above-mentioned embodiments may be modified or some technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiments of the present utility model.
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| CN202420634123.XU CN222564934U (en) | 2024-03-28 | 2024-03-28 | Synchronous separation system for cow milk protein components |
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| CN202420634123.XU CN222564934U (en) | 2024-03-28 | 2024-03-28 | Synchronous separation system for cow milk protein components |
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