CN222274571U - A sealed PCR test kit - Google Patents

Abstract

The utility model provides a PCR sealing detection box which comprises a PCR micro-fluidic chip main body and a chip supporting plate, wherein the PCR micro-fluidic chip main body comprises a substrate and a cover plate which are mutually attached, at least one reaction chamber is formed on the substrate, sample injection holes are respectively formed in the cover plate at the end positions corresponding to the reaction chambers, a groove is formed in the second surface of the chip supporting plate and is used as a chip reserved area, and the PCR micro-fluidic chip main body is embedded and fixed in the chip reserved area in a size-adaptive mode. The PCR sealing detection box provided by the utility model simplifies and automates sample loading and sealing of the PCR microfluidic chip, and is more suitable for being integrated into automatic PCR detection equipment.

Description

PCR sealing detection box

Technical Field

The utility model belongs to the technical field of biomedical detection, and particularly relates to a PCR (polymerase chain reaction) sealing detection box.

Background

At present, when pathogen detection is carried out, samples are sent to a laboratory and subjected to PCR (polymerase chain reaction) reaction by a professional technician by using a large instrument, and the processes of liquid preparation, sample addition, uniform mixing, machine loading and the like are required to be carried out manually, so that the work is complex, the requirements on detection environment and technicians are high, and sample pollution is easy to occur, so that the accuracy of results is reduced.

In addition, the usual PCR amplification carrier is generally a 96-well plate, and automatic film-coating sealing can be realized. However, when the number of samples is relatively small, a manual cover is required to close the PCR tube and the eight-connecting tube, and no good automatic cover sealing mode exists at present. When the microfluidic chip is used as a reaction carrier and a detection container, the microfluidic chip is currently provided with a plurality of sealing modes, namely (1) a cover is provided, manual sealing is needed, (2) a rubber belt-like article is pasted, a sample inlet is sealed, and (3) the integrated card box has a sealing function. The former two modes have high dependence on manual operation and cannot be suitable for automatic products. The third method is mostly used for detecting single sample, the detection flux is low, and the number of single detection targets is limited.

Therefore, there is a need for a PCR cartridge that can be integrated into an automated PCR detection device to achieve automatic sample addition, automatic sealing, and automatic detection.

Disclosure of utility model

In order to solve at least one of the above technical problems, the utility model provides a PCR sealing detection box, which is carried in PCR integrated automatic detection equipment, and a user can automatically complete the whole processes of sample sampling, nucleic acid extraction, reagent preparation, sample loading to a chip, sealing the chip, real-time fluorescence PCR detection and the like by the detection equipment only by putting a sample and consumable into a designated position of the detection equipment, so that the automatic detection of a plurality of samples can be realized once.

The PCR sealing detection box comprises a PCR micro-fluidic chip main body and a chip supporting plate, wherein the PCR micro-fluidic chip main body comprises a substrate and a cover plate which are mutually attached, at least one reaction chamber is formed on the substrate, sample injection holes are respectively formed in the position, opposite to the end parts of each reaction chamber, of the cover plate, a groove is formed in the second surface of the chip supporting plate and used as a chip reserved area, and the PCR micro-fluidic chip main body is embedded in the chip reserved area in a size-adaptive mode.

Further, at least one guide hole is formed in the first surface of the chip supporting plate, and the guide holes are aligned with the sample injection holes of the PCR microfluidic chip main body one by one.

Further, the chip supporting plate further comprises at least one sealant groove, wherein the at least one sealant groove is arranged between the at least one guide hole and the corresponding sample injection hole and is respectively communicated with the corresponding guide hole and the sample injection hole, and the sealant groove is used for accommodating sealant added from the guide hole so as to seal the sample injection hole.

Further, the chip supporting plate further comprises an observation window, and the observation window is arranged in a region, corresponding to the reaction chamber of the PCR micro-fluidic chip main body, of the first surface of the chip supporting plate.

Further, the chip supporting plate further comprises a supporting plate mounting position marking pad, and the marking pad is used for positioning the PCR sealing detection box and the PCR detection equipment when the PCR sealing detection box is loaded to the PCR detection equipment.

Further, the pallet installation position marking pad is positioned through magnetic force.

Furthermore, the at least one sealant groove is a communicated groove at one end of the at least one reaction chamber, and the mode that the lower part of each guide hole corresponds to an independent sealant groove is not adopted, so that the coverage area of sealant can be increased, the contact area between the sealant and the surface of the main body of the PCR microfluidic chip is increased, the sealant is sealed more firmly, and liquid leakage is avoided.

Further, a plurality of grooves are arranged on the chip supporting plate in an array mode to serve as chip reserved areas, and each chip reserved area is embedded with one PCR microfluidic chip main body.

Further, the depth of the groove of the chip reserved area is smaller than the thickness of the main body of the PCR micro-fluidic chip, so that after the main body of the PCR micro-fluidic chip is loaded into the groove from the second surface of the chip supporting plate, the bottom of the main body of the PCR micro-fluidic chip protrudes out from the second surface of the chip supporting plate, the bottom of the PCR sealing detection box is the bottom of the main body of the PCR micro-fluidic chip, the structure of the bottom which keeps flat is more beneficial to the cyclic heating in the PCR detection process, and a special heating plate which is suitable for the uneven bottom is not required to be additionally designed in the PCR detection equipment.

Compared with the prior art, the utility model has the beneficial effects that:

(1) The PCR sealing detection box provided by the utility model can be convenient for a user to package and use the main body of the PCR micro-fluidic chip, and is simultaneously suitable for a micro-fluidic chip with manual sample adding and automatic PCR detection. The PCR micro-fluidic chip can be used alone or in combination, can be used by a user for manually adding samples and then is arranged on a matched PCR instrument for detection, and can be used with full-automatic detection equipment, so that the problems of automatic sample adding, automatic sealing and automatic detection in POCT full-automatic instant detection are solved. The user only needs to put the PCR microfluidic chip into the chip supporting plate and put the chip supporting plate into the fixed clamping position of the PCR detection equipment, the whole process of sample sampling, nucleic acid extraction, reagent preparation, sample adding to the chip, chip sealing and real-time fluorescence PCR detection can be automatically completed by the detection equipment, the automatic detection of a plurality of samples can be realized once, and complicated work such as sample adding and sealing by a professional technician is reduced;

(2) The PCR sealing detection box provided by the utility model simplifies and automates the sample loading of the PCR microfluidic chip, and is more suitable for being integrated into automatic PCR detection equipment;

(3) According to the embodiment of the utility model, the groove is formed in the back surface (the second surface) of the chip supporting plate, so that the PCR microfluidic chip main body is embedded and fixed in the groove in a size-adaptive manner in a dispensing manner, the whole PCR sealing detection box is integrated, the bottom of the PCR sealing detection box is the bottom of the PCR microfluidic chip main body, the structure of the bottom which is kept flat is more beneficial to cyclic heating in the PCR detection process, namely, a special heating plate which is suitable for uneven bottom is not required to be additionally designed in the PCR detection equipment;

(4) According to the embodiment of the utility model, the guide hole is arranged on the chip supporting plate for the liquid-transferring gun head of the automatic liquid-transferring device, so that the error of the liquid-transferring gun head is corrected, the situation that the liquid-transferring gun head is not aligned with the sample inlet of the PCR microfluidic chip when the automatic liquid-transferring device is used for loading samples to the PCR microfluidic chip is avoided, and the stability of sample feeding and sample loading without leakage are ensured;

(5) According to the embodiment of the utility model, the hidden sealant groove is arranged in the chip supporting plate so as to store sealant, so that the sealing performance of the sealant after the sample is added into the PCR microfluidic chip is ensured to be stable, and the sealing effect is achieved;

(6) According to the embodiment of the utility model, the expandable PCR micro-fluidic chip array is designed on the chip supporting plate, so that a plurality of PCR micro-fluidic chips can be loaded simultaneously, and the high-throughput detection of the PCR micro-fluidic chips is realized.

Drawings

FIG. 1 is a schematic top view of a PCR sealing cartridge according to embodiment 1 of the present utility model;

FIG. 2 is a schematic cross-sectional view of a PCR sealing cartridge according to example 1 of the present utility model;

FIG. 3 is an exploded view showing the structure of a PCR sealing cartridge according to embodiment 1 of the present utility model;

FIG. 4 is a schematic view showing the back structure of a chip carrier of a PCR sealing cartridge according to embodiment 1 of the present utility model;

Fig. 5 is a schematic structural diagram of a PCR sealing detection cartridge with a microfluidic chip array according to embodiment 2 of the present utility model.

In the figure:

The device comprises a 1-PCR micro-fluidic chip main body, a 2-chip supporting plate, a 3-reaction chamber, a 4-sample injection hole, a 5-chip reserved area, a 7-guide hole, an 8-sealant groove, a 9-observation window and a 10-supporting plate mounting position marking pad.

Detailed Description

The utility model is further described below with reference to the accompanying drawings.

Example 1

The utility model provides a PCR sealing detection box, and FIGS. 1-2 are respectively schematic top view structure diagrams and schematic cross-sectional structure diagrams of the PCR sealing detection box of the embodiment 1 of the utility model. FIG. 3 is an exploded schematic view of the structure of a PCR sealing cartridge according to example 1 of the present utility model. As shown in fig. 1 to 3, the PCR sealing detection cartridge includes a PCR microfluidic chip body 1 and a chip carrier 2. The PCR microfluidic chip body 1 is formed by bonding (bonding, for example) a substrate (silicon substrate in this embodiment) and a cover plate (glass slide in this embodiment), and then cutting, a plurality of grooves are formed on the silicon substrate by etching to serve as reaction chambers 3, and sample injection holes 4 are formed on the glass slide at two ends opposite to each reaction chamber 3 by, for example, laser drilling. In this embodiment, the chip carrier 2 may be a PC (polycarbonate) or PE (polyethylene) polymer material. FIG. 4 is a schematic diagram showing the back surface structure of a chip carrier of a PCR sealing cartridge according to embodiment 1 of the present utility model. As shown in fig. 4, the second surface of the chip supporting plate 2 is provided with a groove as a chip reserved area 5, and the pcr microfluidic chip body 1 is embedded and fixed in the chip reserved area 5 in a size-adaptive manner. In this embodiment, the frame size of the chip reserved area 5 is adapted to the size of the PCR microfluidic chip body 1, so that the PCR microfluidic chip body 1 can be just embedded into the groove of the chip reserved area 5, and the microfluidic chip body 1 is fixed in the groove by the glue at the four corners of the groove. Preferably, the four corners of the groove of the chip reserved area 5 are designed to be round corners, so that the glue dispensing is more facilitated. The groove is formed in the back surface (the second surface) of the chip supporting plate 2 so as to be embedded into the PCR micro-fluidic chip main body 1, so that the whole PCR sealing detection box is integrated, the bottom of the PCR sealing detection box is the bottom of the PCR micro-fluidic chip main body 1, the structure of the flat bottom is kept more beneficial to cyclic heating in the PCR detection process, and special heating plates adapting to the uneven bottom are not needed to be additionally designed in PCR detection equipment.

As shown in fig. 1, 2 and 4, the front surface (first surface) of the chip supporting plate 2 is provided with a plurality of guide holes 7, and the guide holes 7 are aligned with the sample injection holes 4 of the PCR microfluidic chip main body 1 one by one in the direction perpendicular to the first and second surfaces of the chip supporting plate 2, that is, the number of the guide holes 7 corresponds to the number of the sample injection holes 4. A sealant groove 8 is further formed below the guide hole 7, namely between the guide hole 7 and the sample injection hole 4, and the sealant groove 8 is respectively communicated with the guide hole 7 and the sample injection hole 4 in the direction vertical to the first surface and the second surface of the chip supporting plate 2, so that sealant added from the guide hole 7 by a pipette tip of a pipette device is contained to seal the sample injection hole 4. In general, the diameter of the guide hole 7 is slightly larger than the size of a pipetting gun head of the pipetting device, so that the pipetting gun head is convenient to fix, the front end of the pipetting gun head can be aligned with the sample injection hole 4, the pipetting gun head can be vertically pressed above the sample injection hole 4, and sample addition is ensured to be free from liquid leakage. By arranging the guide hole 7 on the chip supporting plate 2 for the pipetting gun head of the automatic pipetting device, the error of the pipetting gun head is corrected, so that the situation that the pipetting gun head is not aligned with the sample inlet 4 of the PCR microfluidic chip when the automatic pipetting device is used for feeding samples to the PCR microfluidic chip main body 1 is avoided, and the sample feeding stability is ensured.

Preferably, as shown in fig. 4, the sealant groove 8 is designed as a communicating groove at one end of the parallel reaction chambers 3, instead of a separate sealant groove under each guide hole 7, so that the coverage area of the sealant can be increased, the contact area between the sealant and the surface of the PCR microfluidic chip main body 1 can be increased, the sealing of the sealant is firmer, and the leakage is avoided. According to the embodiment of the utility model, the hidden sealant groove 8 is arranged in the chip supporting plate 2 to store sealant, so that the sealing performance of the sealant after the sample is added into the PCR microfluidic chip main body 1 is ensured to be stable, and the sealing effect is achieved.

Further, as shown in fig. 2, an observation window 9 is further formed on the chip supporting plate 2, and the observation window 9 is formed on the front surface of the chip supporting plate 2 in a region corresponding to the reaction chamber 3 of the PCR microfluidic chip main body 1. That is, in this embodiment, the area of the back surface of the chip supporting plate 2 embedded with the PCR micro-fluidic chip main body 1 is hollowed out, and after the PCR micro-fluidic chip main body 1 is embedded into the chip supporting plate 2 and fixed by dispensing, the sample injection holes 4 on the PCR micro-fluidic chip main body 1 are aligned with the guide holes 7 of the chip supporting plate 2 one by one, and the area of the reaction chamber 3 is just located in the hollowed-out area (the observation window 9) to be exposed, so that fluorescence detection data can be conveniently obtained from the observation window 9.

Further, as shown in fig. 1, the chip pallet 2 further includes a pallet mounting position identification pad 10 for positioning with the PCR detection apparatus when the PCR sealing detection cartridge is loaded into the PCR detection apparatus. For example, in this embodiment, the pallet mounting position marking pads 10 are respectively disposed at four corners of the chip pallet 2, specifically, the pallet mounting position marking pads 10 can be positioned by magnetic force, and the pallet mounting position marking pads 10 are made of metal iron, for example, and are automatically positioned under the action of magnetic attraction with magnetic force sheets disposed at specific positions on the PCR detection device.

The working process of the PCR sealing detection box provided by the embodiment of the utility model is that firstly, the PCR sealing detection box (the observation window 9 on the chip supporting plate 2 is upward) is loaded on the adaptive PCR detection equipment, the main body of the PCR micro-fluidic chip is automatically fixed under the action of the adsorption force of the PCR detection equipment, and the heating positions of the main body 1 of the PCR micro-fluidic chip and the PCR detection equipment are completely attached, so that the heat conduction is convenient. When the magnetic force positioning is used, magnets at corresponding positions are reserved on the PCR detection equipment, and the support plate mounting position marking pad 10 can be adsorbed and aligned and positioned on the POCT all-in-one machine simultaneously through the magnetic force sheet 11. According to the program flow, the matched gun head is grabbed by a pipetting mechanical arm on the PCR detection equipment, the prepared PCR detection reagent is sucked, and the gun head is aligned to the sample injection hole 4 of the PCR micro-fluidic chip main body 1 through the gun head guide hole 7. Because some gun heads are bent and not completely vertical, position errors are formed between the gun heads and the sample injection holes 4, the errors can be corrected through the guide holes 7, pressure is applied through the pipettor, the front ends of the gun heads are tightly attached to the sample injection holes 4 of the PCR microfluidic chip main body 1, liquid in the gun heads is pushed to flow into the PCR microfluidic chip main body 1 through air pressure in the pipettor to finish sample injection, the gun heads are replaced after sample injection, the gun heads are used for dispensing glue to the lower sealant groove 8 through the guide holes 7 after sealant is sucked, the sample injection holes 4 of the PCR microfluidic chip main body 1 are sealed through ultraviolet curing sealant, the PCR amplification detection is started after the sealing is finished, fluorescent detection data are obtained through the observation window 9 on the chip supporting plate 2, and data analysis is finished, so that a detection result is obtained.

Example 2

Fig. 5 is a schematic structural diagram of a PCR sealing detection cartridge with a microfluidic chip array according to embodiment 2 of the present utility model. In the present embodiment, a plurality of PCR microfluidic chip bodies 1 are arranged in an array on a chip carrier 2. For example, as shown in fig. 5, four-way (2×2 array) and eight-way (4*2 array) are used, and in eight-way arrangement, a total of 8 3-channel PCR microfluidic chip bodies 1 can realize PCR detection of 24 samples.

It should be noted that the above-mentioned embodiments are merely preferred embodiments of the present utility model, and the present utility model is not limited thereto, but may be modified or substituted for some of the technical features thereof by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (9)

1. The PCR sealing detection box is characterized by comprising a PCR micro-fluidic chip main body (1) and a chip supporting plate (2), wherein the PCR micro-fluidic chip main body (1) comprises a substrate and a cover plate which are mutually attached, at least one reaction chamber (3) is formed on the substrate, sample injection holes (4) are respectively formed on the cover plate at the end positions corresponding to the reaction chambers (3), grooves are formed on the second surface of the chip supporting plate (2) and serve as chip reserved areas (5), and the PCR micro-fluidic chip main body (1) is embedded and fixed in the chip reserved areas (5) in a size-adaptive mode.

2. The PCR sealing detection cartridge as claimed in claim 1, wherein the first surface of the chip supporting plate (2) is provided with at least one guide hole (7), and the guide holes (7) are aligned with the sample injection holes (4) of the PCR microfluidic chip main body (1) one by one.

3. The PCR sealing cartridge of claim 2, wherein the chip carrier (2) further comprises at least one sealant groove (8), the at least one sealant groove (8) being provided between the at least one guide hole (7) and the corresponding sample injection hole (4) and being respectively communicated with the corresponding guide hole (7) and sample injection hole (4) for receiving sealant added from the guide hole (7) to seal the sample injection hole (4).

4. The PCR sealing cartridge as claimed in claim 2, wherein the chip carrier (2) further comprises a viewing window (9), the viewing window (9) being open at a region of the first surface of the chip carrier (2) corresponding to the reaction chamber (3) of the PCR microfluidic chip body (1).

5. The PCR sealing cartridge of claim 1, wherein the chip carrier (2) further comprises a carrier mounting location identification pad (10) for positioning with the PCR detection device when the PCR sealing cartridge is loaded into the PCR detection device.

6. The PCR sealing cartridge of claim 5, wherein the pallet mounting location indicator pad (10) is magnetically positioned.

7. A PCR sealing cartridge according to claim 3, characterized in that the at least one sealant groove (8) is a communicating groove at one end of the at least one reaction chamber (3).

8. The PCR sealing cartridge as claimed in claim 1, wherein a plurality of said recesses are arranged in an array on said chip carrier (2) as said chip reserved areas (5), one of said PCR microfluidic chip bodies (1) being embedded in each of said chip reserved areas (5).

9. The PCR sealing cartridge according to claim 1, wherein the depth of the recess of the chip pre-reserved area (5) is smaller than the thickness of the PCR microfluidic chip body (1).