Agglutination experiment board and board frame thereof
Technical Field
The utility model relates to the technical field of biological experimental tools for preventing and controlling animal diseases, in particular to an agglutination experimental plate and a plate frame thereof.
Background
The agglutination test is a common detection method for preventing, diagnosing and monitoring diseases by detection technicians, and the principle is that according to the specific binding characteristics of antigen and antibody, the interaction between a detected sample and a detection reagent component is caused to generate agglutination particles or flocculent precipitates, for example, the agglutination phenomenon occurs when the particle antigens such as bacteria, red blood cells and the like are directly combined with corresponding antibodies, and the detected sample is judged to be positive or negative through the generation of the agglutination particles or flocculent precipitates, so that the infection condition or the antibody level of the sampled animal is judged. Through the agglutination experiment, animal diseases can be rapidly, accurately and effectively identified, animal infection states or immune states can be judged, clinical diagnosis and epidemiological investigation and research are assisted, so that the animals can be treated in the later period, and diseases can be purified and the like.
Agglutination experiments are classified into a slide method, in which a glass plate is used as a main experimental tool, and a tube agglutination method, in which a test tube and a tube rack are used as a main experimental tool. The existing glass-type agglutination experiment flat products comprise glass plates, disposable agglutination experiment cards, 96-hole disposable agglutination experiment plates and the like. The glass plate is convenient to use, good in result observation and unobvious in liquid adsorption, but easy to damage, the inclined glass plate is easy to flow out, the glass plate is thick and heavy in material, and production is not seen in the market at present. The disposable agglutination test card has more problems in the actual use process, and is mainly characterized in that the test card is easy to bend and move so that the liquid is easy to flow or cause pollution, the test card is opaque, the liquid is easy to aggregate after sample addition, the observation is inconvenient, the liquid loss is large after sample addition due to the adsorption of the paper board, and the like. Although the 96-well disposable agglutination experimental plate has a high-throughput design, the 96-well reaction holes are too many, and resource waste is easily caused for detecting a small amount of samples. The test tube that test tube agglutination experiment used is mainly glass products, damages easily, and test tube and test-tube rack occupation space are big, also inconvenient image retention work such as shooing of later stage.
Therefore, it is necessary to design an experimental apparatus which is convenient, durable and convenient to observe as a reaction carrier for agglutination experiments, so as to solve the problems of heavy weight, easy damage, easy flowing or adsorption of liquid, inconvenient observation, resource waste and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides an agglutination experiment plate and a plate frame thereof, which solve the problems that the glass plate is easy to damage, the liquid is easy to flow out and is thick and heavy, the disposable agglutination experiment card is easy to bend and move to cause liquid flowing or pollution, the result is inconvenient to observe, the liquid loss after sample addition is large, the 96-hole disposable agglutination experiment plate is easy to cause resource waste, the test tube is easy to damage, the occupation space of the test tube and the test tube frame is large, the later photographing is inconvenient, and the like.
The utility model is realized by the following technical scheme that the agglutination experiment plate and the plate frame thereof comprise an agglutination experiment reaction plate and the plate frame. Further, the reaction plate is of a transparent whole body structure, two sides of the upper end of the reaction plate are provided with ear-shaped limiting and fixing devices, and the upper surface of each ear-shaped limiting and fixing device is a marking surface of a ground glass-shaped plane structure. Further, the reaction plate is provided with a plurality of reaction holes which are arranged in an array, and preferably, the reaction holes are of a hollow and concave vertical cuboid structure. Further, the grillage comprises grillage panel and strengthening rib, and the grillage panel is transparent material, and its upper end array arranges a plurality of spacing fixed slot, and four angles of grillage panel are connected by four strengthening ribs.
The utility model provides an agglutination experiment plate and a plate frame thereof, which have the following beneficial effects.
This agglutination test plate and grillage thereof through the vertical flat cuboid formula reaction hole of design cavity, can make things convenient for the mixture of experimental component, and the reaction result can very audio-visual large tracts of land observe, and further, the vertical flat reaction hole design can effectively block the loss or the outflow of experimental component liquid.
The agglutination experiment plate and the plate frame thereof can conveniently fix the agglutination experiment plate on the plate frame through the ear-type limiting and fixing devices on the two sides of the reaction plate and the limiting and fixing clamping grooves on the plate frame. Further, the grillage is rectangle three-dimensional structure, can firmly carry the agglutination experiment board and avoid empting to the space except that the strengthening rib is connected between the grillage panel is hollow out construction, can make things convenient for the observation of experimental result.
The agglutination experiment plate and the plate frame thereof are provided with a plurality of reaction holes, which is convenient for comparison and observation. Further, a plurality of agglutination experiment plates can be placed on the plate frame according to the quantity of the detected samples, so that a large number of sample detection requirements are met, and meanwhile, resource waste is reduced.
The agglutination experiment plate and the plate frame thereof are provided with marking surfaces on the surfaces of ear-type limiting and fixing devices at two ends of the reaction plate. Furthermore, the marking surface is of a ground glass-shaped plane structure, so that experimental marking can be conveniently carried out, and the marking symbol is not easy to wipe by the plane design.
The agglutination experiment plate and the plate frame thereof have certain spacing between the limiting fixing clamping grooves on the plate frame, and can meet the high-flux detection of agglutination experiments by being matched with the multichannel pipettor with adjustable spacing. Furthermore, a common pure-color control bottom plate can be additionally arranged in a reserved gap between two adjacent reaction plates, so that the observation of experimental results is more convenient.
The agglutination experiment plate and the plate frame thereof are made of novel materials which are transparent, wear-resistant and reusable, and can be reused by cleaning after being used, so that the experiment cost is reduced, and the resource waste is reduced.
Drawings
FIG. 1 is a schematic view of the overall structure of an agglutination assay plate and a plate rack thereof according to the present utility model;
FIG. 2 is a schematic perspective view of the whole structure of the reaction plate of the present utility model;
FIG. 3 is a schematic view of the overall structure of the pallet of the present utility model.
In the figure, a reaction plate, a plate frame, a lug type limiting and fixing device, a marking surface, a 103, a reaction hole, a 201, a reinforcing rib, a 202, a plate frame panel and a 203, wherein the limiting and fixing slot is formed in the plate frame.
Detailed description of the preferred embodiments.
The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to FIGS. 1 to 3, the utility model provides a technical scheme that an agglutination experiment plate and a plate frame thereof comprise a reaction plate (1) and a plate frame (2). Further, the reaction plate (1) is provided with a plurality of reaction holes (103) which are arranged in an array, and the reaction holes (103) are of a hollow and concave vertical cuboid structure. Further, the opening of the reaction hole (103) is of a flat rectangular structure and is flush with the upper surface of the reaction plate (1), so that the agglutination experimental result can be observed very conveniently, the agglutination condition is visually and three-dimensionally displayed in a large area, the comparison of a plurality of sample detection and experimental results is met, meanwhile, the liquid in the reaction hole (103) can be effectively prevented from flowing out, and the liquid loss of experimental components is reduced. Further, the sealing plate membrane is covered on the reaction plate (1) to prevent the experimental components in the reaction holes (103) from losing. Further, the two sides of the upper end of the reaction plate (1) are provided with the ear-type limiting and fixing devices (101), and the reaction plate (1) can be stably placed in the plate frame (2) and is not easy to fall through the ear-type limiting and fixing devices (101) and the limiting and fixing clamping grooves (203) on the plate frame (2). Further, the upper surface of the ear-type limiting and fixing device (101) is provided with a marking surface (102), and the marking surface (102) is of a ground glass-like plane structure, so that the marking of a detection person can be facilitated, and the detection person is not easy to wipe off.
Further, the grillage (2) is rectangular three-dimensional structure, and strengthening rib (201) are connected with grillage panel (2) with the vertical direction, and strengthening rib (201) surface flushes with grillage panel (202) surface that is connected, can make grillage (2) firmly place be difficult for empting on the test bench. Further, the plate frame (2) is provided with a plurality of limiting fixing clamping grooves (203), so that the placing requirements of a plurality of reaction plates can be met simultaneously, and a plurality of samples can be detected simultaneously. Furthermore, the space between the plate frame panels (202) except for the connection of the reinforcing ribs (202) is of a hollow structure, so that the experimental results of the reaction plate (1) can be conveniently observed and compared.
Further, the material of the agglutination test plate is a novel material which is transparent, abrasion-resistant and reusable, such as TPU, engineering plastics, resin, renewable polycarbonate and the like.
In the present utility model, the steps of using the agglutination assay plate and the plate rack thereof are as follows.
Firstly placing the plate frame on an experiment table, taking out the reaction plate, loading the reaction plate in the plate frame, sucking the detection reagent into the reaction hole, sucking the sample to be detected into the reaction hole, and uniformly stirring and mixing by using a suction head or a mixing device, or slightly shaking the plate frame to fully mix the detection reagent and the sample to be detected, and controlling the volume of liquid in the reaction hole according to different experiment specifications.
Then, a sealing plate film, a glass plate or other materials which are commonly used in laboratories and can be used for preventing liquid loss are covered on the upper surface of the reaction plate, and the reaction plate is stood until the time requirement specified by the experimental detection standard is met.
Finally, directly observing the agglutination test result, or shaking the plate frame to observe the agglutination condition, or taking out the reaction plate to observe the agglutination condition, or adding a solid-color baffle or light source on the back of the reaction plate, so as to more clearly observe the agglutination condition and shoot the test result image data.
If the samples to be detected are more, a plurality of plate frames can be used, and a plurality of reaction plates are placed on the plate frames, so that the requirement of detecting a large number of samples simultaneously can be met.
After the experiment is completed and the reaction plate is used, the experimental components in the reaction hole are treated according to the laboratory waste treatment regulation, and the reaction plate is washed or cleaned by ultrasonic waves and can be reused after being dried or dried.
It should be understood that while exemplary embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made hereto without departing from the spirit and scope of the utility model as defined by the appended claims and their equivalents.