CN222050011U - Fluorescent rapid detection chip - Google Patents

Abstract

The utility model relates to the technical field of fluorescence detection, and particularly discloses a fluorescence rapid detection chip, which comprises a shell, wherein an excitation light source is arranged on the shell, a dispersing mechanism for dividing laser into a plurality of groups is arranged on the excitation light source, and the dispersing mechanism is positioned in the shell; the dispersing mechanism comprises a plurality of groups of second connecting sleeves, fluorescent filters and sample dishes are arranged below the plurality of groups of second connecting sleeves, the plurality of groups of fluorescent filters are respectively aligned with the plurality of groups of sample dishes, and the fluorescent filters are positioned above the sample dishes; the method solves the technical problems that only one group of detection samples can be detected at the same time, and when different pathogens need to be detected on the same sample, a large amount of time is consumed, so that the detection efficiency is reduced.

Description

Fluorescent rapid detection chip

Technical Field

The utility model relates to the technical field of fluorescence detection, and particularly discloses a fluorescence rapid detection chip.

Background

The fluorescence detection generally needs an excitation light source, a fluorescence filter, a sample cabin and a fluorescence detector, the excitation light source is used for exciting fluorescent substances in a detection sample in the sample cabin, and the detection sample is placed in the fluorescence detector for detection, so that a detection result is obtained; the fluorescence detection method has wide application in the fields of biological medicine, environmental monitoring, food safety and the like. The method can observe cells or tissues in the biomedical field, can detect pathogens rapidly, and has higher accuracy and higher practicability than that of a macroscopic observation method.

The invention patent with the patent number of CN114878527A discloses a fluorescence detection system for a biochip, which comprises a bracket and an imaging acquisition component vertically arranged on the bracket, wherein a first optical filter is arranged below the imaging acquisition component; a light source assembly is arranged below the first optical filter, the light source assembly comprises a light source rotary table, the light source rotary table is coaxial with the imaging acquisition assembly, and a white light source is arranged in the center of the light source rotary table; the light source turntable is provided with a plurality of pairs of monochromatic light sources, each pair of monochromatic light sources is symmetrical about the center of the light source turntable, the light-emitting end of the monochromatic light source points to the position right above the white light source, the light-emitting end of the monochromatic light source is provided with a second optical filter, and a light homogenizing component is arranged between the monochromatic light source and the second optical filter; when detecting a detection sample, the existing fluorescence detection chip can rapidly switch a fluorescence filter and the detection sample, but can only detect one group of detection samples at the same time, and when detecting different pathogens of the same sample, a great deal of time is required to be consumed, so that the detection efficiency is reduced.

Disclosure of utility model

Therefore, the present utility model is directed to a fluorescent rapid detection chip, so as to solve the technical problem that a group of detection samples can be detected at the same time, and when detection of different pathogens is required to be performed on the same sample, a lot of time is required, thereby reducing the detection efficiency.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the fluorescent rapid detection chip comprises a shell, wherein an excitation light source is arranged on the shell, a dispersing mechanism for dividing laser into a plurality of groups is arranged on the excitation light source, and the dispersing mechanism is positioned in the shell; the dispersing mechanism comprises a plurality of groups of second connecting sleeves, fluorescent filters and sample dishes are arranged below the second connecting sleeves, the fluorescent filters are aligned with the sample dishes, and the fluorescent filters are located above the sample dishes. The laser is emitted by the excitation light source, the light source is dispersed into a plurality of beams by the dispersing mechanism, and a plurality of groups of sample dishes are simultaneously detected, so that the detection time is shortened, and the detection efficiency is improved.

Further, the dispersing mechanism comprises a first connecting sleeve, the first connecting sleeve is sleeved on the excitation light source, a plurality of light splitting blocks are arranged in the first connecting sleeve, a first mirror surface is clamped between every two adjacent light splitting blocks, a plurality of second connecting sleeves are fixedly connected to the first connecting sleeve and communicated with the first connecting sleeve, a plurality of second connecting sleeves are uniformly distributed in a ring shape, and a second mirror surface is arranged in the first connecting sleeve. The laser emitted from the excitation light source is dispersed into a plurality of groups of lasers in the first connecting sleeve, and the lasers are dispersed into the second connecting sleeve through the first mirror reflection, so that a plurality of groups of detection samples can be detected simultaneously, and the detection efficiency is improved.

Further, the first mirror surface and the second mirror surface are inclined at 45 degrees, and the first mirror surfaces of a plurality of groups are respectively parallel to the second mirror surfaces of a plurality of corresponding groups. The light beams are refracted through the first mirror surface and the second mirror surface, so that a certain distance is reserved between two groups of adjacent light beams, a plurality of groups of sample dishes are detected simultaneously, and the detection efficiency is improved.

Further, the bottom of the first connecting sleeve is provided with a fixed column, two groups of first connecting rods are fixedly connected to the fixed column, a fixed ring is arranged on the two groups of first connecting rods, and a plurality of groups of fluorescent filters are clamped on the fixed ring. The fluorescence filter is used for filtering part of wavelengths of the laser, so that fluorescent substances of specified pathogens in a detection sample can be excited, the purpose of detection is achieved, and meanwhile, the accuracy is guaranteed.

Further, a plurality of groups of second connecting rods are arranged on the fixing columns, light beams are arranged on the plurality of groups of second connecting rods, and the plurality of groups of light beams are respectively positioned between the plurality of groups of fluorescent filters and the sample vessel. The beam light cylinder is used for mode laser scattering, so that the detection result of adjacent detection samples is affected.

Further, the lower end of the fixed column is provided with a slidable movable ring, and a plurality of groups of sample dishes are placed on the movable ring; the fixed column bottom fixedly connected with link, fixedly connected with two sets of sliding blocks on the link, be provided with two sets of fixed blocks on the expansion ring, two sets of the sliding block slip clamps respectively two sets of on the fixed block, one of them end of fixed block is provided with first dog, and the other end is provided with the second dog. Through sliding block and link cooperation, make the expansion link can be in the casing internal sliding, area of contact between sliding block and the link is great, so can guarantee that the solid fixed ring can steadily slide, can not appear the condition of slope.

Further, a fixing frame is arranged in the shell, a fixing ring is arranged on the first connecting sleeve, and the fixing ring is clamped on the fixing frame in a sliding manner. The fixing frame is matched with the fixing ring to fix the dispersing mechanism in the shell.

Further, a sealing block is slidably clamped on the shell, a rectangular block is arranged on the sealing block, and the movable ring is clamped on the rectangular block. Through sealing block and rectangular block cooperation, can pull out the expansion ring from the casing to change the sample dish.

The working principle and the beneficial effects of the scheme are as follows:

When the fluorescent light detection device is used, an excitation light source is started firstly, the excitation light source emits laser, the laser is dispersed into a plurality of groups of light beams through a dispersing mechanism, the plurality of groups of light beams respectively pass through a plurality of groups of fluorescent filters, so that the wavelengths of the light beams are filtered, light with a specified wavelength is remained, the light beams pass through a beam light cylinder to irradiate on a sample dish, a detection sample is irradiated, fluorescent substances in the detection sample are excited, the irradiated detection sample is placed in a fluorescent detector, and whether the detection sample contains the pathogen is judged by detecting whether the detection sample contains the fluorescent light; the detection can be carried out on a plurality of groups of sample dishes simultaneously, so that the detection time is shortened, and the detection efficiency is improved.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model. The objects and other advantages of the utility model may be realized and obtained by means of the instrumentalities and combinations particularly pointed out in the specification.

Drawings

FIG. 1 is a schematic diagram of an embodiment;

FIG. 2 is a schematic diagram of the internal structure of an embodiment;

FIG. 3 is an exploded view of the internal structure of the embodiment;

Fig. 4 is a cross-sectional view of an embodiment.

The figures are marked as follows: the light source device comprises a body 1, an excitation light source 2, a first connecting sleeve 3, a fixed ring 4, a second connecting sleeve 5, a light splitting block 6, a first mirror 7, a second mirror 8, a fixed ring 9, a fluorescent filter 10, a fixed column 11, a first connecting rod 12, a second connecting rod 13, a light beam barrel 14, a movable ring 15, a fixed groove 16, a sample dish 17, a connecting frame 18, a sliding block 19, a limiting groove 20, a fixed block 21, a sliding groove 22, a limiting block 23, a first blocking block 24, a second blocking block 25, a sealing block 26, a rectangular block 27, a clamping groove 28, a handle 29 and a fixing frame 30.

Detailed Description

The following is a further detailed description of the embodiments:

Examples

As shown in fig. 1 to 4, a fluorescent rapid detection chip is disclosed, which comprises a shell 1, wherein an excitation light source 2 is clamped on the shell 1, a dispersing mechanism is arranged on the excitation light source 2, the dispersing mechanism comprises a first connecting sleeve 3, the first connecting sleeve 3 is rotatably connected with the shell 1, a plurality of groups of light blocks 6 are arranged in the first connecting sleeve 3, the groups of light blocks 6 are annularly arranged in an array manner, one end of the first connecting sleeve 3 is fixedly connected with a plurality of groups of second connecting sleeves 5, the second connecting sleeves 5 are annularly arranged around the first connecting sleeve 3, a first mirror surface 7 is arranged at the communication position of the first connecting sleeve 3 and the second connecting sleeve 5, the first mirror surface 7 is inclined at 45 degrees and clamped between two groups of adjacent light blocks 6, the second connecting sleeve 5 is L-shaped, a second mirror surface 8 is arranged at the corner of the second connecting sleeve 5, and the second mirror surface 8 is inclined at 45 degrees; the first mirror surface 7 is triangular, so that the first mirror surface 7 is conveniently clamped between two groups of adjacent light splitting blocks 6, the second mirror surface 8 is rectangular, and the second mirror surface 8 is conveniently clamped at the corner of the second connecting sleeve 5; the shell 1 is provided with a fixing frame 30, the first connecting sleeve 3 is fixedly connected with a fixing ring 4, and the fixing ring 4 is slidably clamped in the fixing frame 30 and is rotatably connected with the fixing frame 30. The excitation light source 2 is a common technical means for the person skilled in the art, and its structure and specific use are all techniques well known to the person skilled in the art.

The bottom end of the first connecting sleeve 3 is fixedly connected with a fixed column 11, a first connecting rod 12 is arranged on the fixed column 11, both ends of the first connecting rod 12 are fixed on the fixed ring 4, a plurality of groups of fluorescent filters 10 are clamped on the fixed ring 4, the plurality of groups of fluorescent filters 10 can respectively filter out fluorescent signals with different wavelengths, the plurality of groups of fluorescent filters 10 are respectively vertically aligned with the plurality of groups of second mirror surfaces 8, and the fluorescent filters 10 are positioned below the second connecting sleeve 5; the fixed column 11 is provided with a plurality of groups of second fixing frames 30 which are annularly arranged, the plurality of groups of second fixing frames 30 are respectively provided with a plurality of groups of light beams 14, the plurality of groups of light beams 14 are respectively vertically aligned with the plurality of groups of fluorescent filters 10, and the light beams 14 are positioned below the fluorescent filters 10. As shown in fig. 2

The bottom of the fixed column 11 is provided with a movable ring 15 which is in sliding connection, the movable ring 15 is provided with a plurality of groups of fixed grooves 16, sample dishes 17 are placed in the plurality of groups of fixed grooves 16, the sample dishes 17 contain detection samples, the plurality of groups of sample dishes 17 are respectively vertically aligned with the plurality of groups of light-beam cylinders 14, and the culture dishes are positioned below the light-beam cylinders 14; the fixed column 11 bottom fixedly connected with link 18, link 18 wherein both ends all are provided with slider 19, fixedly connected with two sets of fixed blocks 21 on the expansion ring 15, fixed slot 16 has been seted up on the fixed block 21, two sets of sliders 19 slip clamps respectively in sliding tray 22, both sides of sliding tray 22 all are provided with stopper 23, two sets of spacing groove 20 have all been seted up at the link 18 both ends, two sets of stopper 23 slip clamps respectively in two sets of corresponding spacing groove 20, fixed block 21 one end is provided with first block piece 24, the other end is provided with second block piece 25, first block piece 24 blocks sliding tray 22 one end, prevent slider 19 slip out fixed block 21 one end, second block piece 25 is used for preventing link 18 and fixed block 21 separation, but slider 19 can follow second block piece 25 one end and slide out fixed block 21, as shown in fig. 3.

The sealing block 26 is slidably clamped on the shell 1, a handle 29 is arranged on one side of the sealing block 26, the handle 29 is positioned outside the shell 1, a rectangular block 27 is arranged on the other side of the sealing block 26, the rectangular block 27 is positioned inside the shell 1, a clamping groove 28 is formed in the rectangular block 27, the cross section of the clamping groove 28 is in a fan shape, and the movable ring 15 is clamped in the clamping groove 28.

In the concrete implementation

When the fluorescent light detection device is used, the excitation light source 2 is started, the excitation light source 2 emits laser, the laser is divided into a plurality of beams by the plurality of component light blocks 6, the beams of laser are respectively deflected by 90 degrees through the first mirror 7 and are refracted to the second mirror 8, the laser is refracted by 90 degrees through the second mirror 8, the laser passes through the fluorescent filter 10, light with a certain wavelength is filtered, the laser passes through the fluorescent filter 10 and then passes through the beam light tube 14 to irradiate the sample dish 17, the irradiated sample is irradiated, the irradiated sample is placed into the fluorescent detector, and whether the sample contains the pathogen is judged by detecting whether the sample contains the pathogen or not; the beam light cylinder 14 can separate a plurality of laser beams and prevent the laser beams from scattering, thereby affecting the detection precision of the adjacent sample dishes 17; the fluorescent filters 10 are matched with the sample containers 17, so that a plurality of groups of fluorescent filters 10 can be used for filtering light with the same wavelength, and detection samples in a plurality of groups of sample containers 17 are different, so that the same pathogen detection is carried out on different detection samples; the sets of fluorescence filters 10 may also be configured to filter light of different wavelengths, with the same test sample in the sets of sample holders 17, and with different pathogens being detected on the same test sample. The laser emitted by the excitation light source 2 is divided into a plurality of beams by the dispersing mechanism, and the plurality of groups of sample dishes 17 are detected simultaneously, so that the detection time can be greatly reduced, and the detection efficiency is improved.

When the sample dish 17 needs to be replaced, the sealing block 26 is pulled out through the handle 29, the sealing block 26 drives the movable ring 15 to move through the rectangular block 27, the movable ring 15 drives the first blocking block 24 to move, the sliding block 19 slides in the sliding groove 22, meanwhile, the limiting block 23 slides in the limiting groove 20, the connecting frame 18 and the sliding block remain motionless until the second blocking block 25 contacts with the connecting frame 18, at the moment, part of the sliding block 19 is positioned outside the sliding groove 22, the movable ring 15 is completely positioned outside the shell 1, and a worker takes out the sample dish 17 from the fixed groove 16; when the sample dish 17 is required to be placed in the shell 1, the sample dish 17 is placed in the fixed groove 16, then the sealing block 26 is pushed to drive the movable ring 15 to move, the sliding block 19 is driven to slide in the sliding groove 22 until the sliding block 19 is contacted with the first blocking block 24, and at the moment, the plurality of groups of sample dishes 17 are aligned with the plurality of groups of light beams 14 respectively, so that the sample dish 17 is installed; the disassembly of the sample dish 17 is simple and convenient, and the staff can quickly replace the sample dish 17, so that the detection efficiency is improved.

The foregoing is merely exemplary embodiments of the present utility model, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present utility model, and these should also be considered as the scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the practical applicability of the present utility model.

Claims (8)

1. A fluorescence rapid detection chip, characterized in that: it comprises a housing, an excitation light source is disposed on the housing, a dispersion mechanism for dividing laser light into a plurality of groups is disposed on the excitation light source, and the dispersion mechanism is located inside the housing; The dispersing mechanism includes a plurality of groups of second connecting sleeves, and fluorescent filters and sample dishes are arranged below the plurality of groups of second connecting sleeves. The plurality of groups of fluorescent filters are respectively aligned with the plurality of groups of sample dishes, and the fluorescent filters are located above the sample dishes. 2. A fluorescence rapid detection chip according to claim 1, characterized in that: the dispersion mechanism includes a first connecting sleeve, the first connecting sleeve is sleeved on the excitation light source, a plurality of component light blocks are arranged in the first connecting sleeve, a first mirror is clamped between every two adjacent groups of the light splitting blocks, a plurality of groups of the second connecting sleeves are fixedly connected to the first connecting sleeve and are connected to the first connecting sleeve, a plurality of groups of the second connecting sleeves are evenly distributed in a ring shape, and a second mirror is arranged in the first connecting sleeve. 3. A fluorescence rapid detection chip according to claim 2, characterized in that: the first mirror surface and the second mirror surface are both inclined at 45 degrees, and several groups of the first mirror surfaces are parallel to several corresponding groups of the second mirror surfaces. 4. A fluorescence rapid detection chip according to claim 3, characterized in that: a fixing column is provided at the bottom end of the first connecting sleeve, two groups of first connecting rods are fixedly connected to the fixing column, and fixing rings are provided on the two groups of the first connecting rods, and several groups of the fluorescence filters are clamped on the fixing rings. 5. A fluorescence rapid detection chip according to claim 4, characterized in that: a plurality of groups of second connecting rods are arranged on the fixed column, a plurality of groups of second connecting rods are each provided with a beam tube, and a plurality of groups of beam tubes are respectively located between a plurality of groups of the fluorescence filters and the sample dish. 6. A fluorescence rapid detection chip according to claim 5, characterized in that: a slidable movable ring is provided at the lower end of the fixed column, and a plurality of groups of sample dishes are placed on the movable ring; The bottom end of the fixed column is fixedly connected to a connecting frame, and two groups of sliding blocks are fixedly connected to the connecting frame. Two groups of fixed blocks are arranged on the movable ring. The two groups of sliding blocks are respectively slidably mounted on the two groups of fixed blocks. A first stop block is arranged at one end of the fixed block, and a second stop block is arranged at the other end. 7. A fluorescence rapid detection chip according to claim 6, characterized in that: a fixing frame is arranged inside the shell, a fixing ring is arranged on the first connecting sleeve, and the fixing ring is slidably mounted on the fixing frame. 8. A fluorescence rapid detection chip according to claim 7, characterized in that: a sealing block is slidably mounted on the shell, a rectangular block is arranged on the sealing block, and the movable ring is mounted on the rectangular block.