CN222050010U - An observation device for detecting transgenic fluorescent protein - Google Patents

Abstract

The utility model discloses an observation device for detecting transgenic fluorescent protein, which comprises a control box, a control panel, an outer box body, a split type box door, an optical filter, a controller, a side radiator, a storage table, a three-band point light source, a rear radiator, a camera and a Z-direction lifting mechanism. The utility model integrally adopts an openable box body structure, and the observed object is closed after being placed in the box, so that the influence of natural light on the observed result can be greatly reduced; the three-band point light source can be used for detecting GFP, YFP, RFP and other fluorescent proteins, and meanwhile, the optical filter is replaced according to the corresponding light source type, so that the use is convenient, and the injury to observers is reduced; the multi-surface light source in the box body is beneficial to reducing the influence of local shadow on observation and reducing the color distortion degree.

Description

A viewing device for transgene fluorescent protein detects

Technical Field

The utility model belongs to the technical field of fluorescent proteins, and particularly relates to an observation device for detecting transgenic fluorescent proteins.

Background

Green fluorescent protein (Green fluorescent protein, GFP for short) is a protein consisting of about 238 amino acids, and was originally isolated from Victoria multi-tube light-emitting jellyfish. GFP can be excited by blue light to ultraviolet light, and has a main excitation peak at 395nm and a secondary excitation peak at 475nm, and the main emission peak emits green fluorescence at 509 nm. GFP is widely used as a molecular marker in cell biology and molecular biology for auxiliary screening due to its fluorescent properties.

When the transgenic fluorescent protein is detected, a fluorescent microscope or a laser flashlight is often used as an excitation light source to irradiate so as to observe and detect the green fluorescent protein, and the existing excitation light source has the following defects:

1. The fluorescence microscope is high in price and operation difficulty;

2. The laser flashlight needs to be observed in a handheld manner, and influences operation when experimental operation and result shooting are needed;

3. Fluorescent microscopes and laser flashlights can be influenced by natural light under indoor environment, and can be accurately observed in dark environment.

For this reason, it is necessary to develop an observation device for detection of transgenic fluorescent proteins capable of solving the above-mentioned problems.

Disclosure of utility model

The utility model aims to provide an observation device which integrates an excitation light source, an observation platform and a target wave band filtering function, is convenient to observe and easy to operate and is used for detecting transgenic fluorescent protein.

The utility model aims at realizing the purposes, the intelligent cabinet temperature control device comprises a control box, a control panel and an outer box body, wherein a controller is arranged in the control box, the control panel is arranged outside the control box, the outer box body is arranged on the control box, a split type box door is arranged on the front surface of the outer box body, a window is formed in the split type box door, a slot is formed in the window, a light filter is placed in the slot, a side radiator and a rear radiator are arranged in the outer box body and used for multi-surface heat dissipation, three-band point light sources are uniformly distributed on the side radiator and the rear radiator respectively, a light scattering sheet is covered on the surfaces of the three-band point light sources, a camera is arranged in the outer box body, a Z-direction lifting mechanism is arranged in the outer box body and drives a storage table to lift, and the controller is electrically connected with the control panel, the three-band point light sources, the camera and the Z-direction lifting mechanism respectively.

Preferably, the Z-direction lifting mechanism comprises a Z-direction idler wheel bracket, a Z-direction lifting synchronous belt, synchronous belt wheels I, synchronous belt wheels II, a Z-direction motor synchronous belt, a Z-direction lifting motor, a Z-axis motor bracket, Z-direction driving synchronous belt 20 belt seat bearings and a Z-direction driving shaft, wherein the bottom in the control box is fixedly provided with the Z-direction idler wheel bracket through bolts, two pairs of belt seat bearings are fixedly arranged in an upper cover at the top of the outer box body, each pair of belt seat bearings is provided with a Z-direction driving shaft, two end surfaces of each Z-direction driving shaft are respectively provided with a synchronous belt wheel I, synchronous belt wheels I corresponding to different Z-direction driving shafts are connected through the Z-direction driving synchronous belt, the synchronous belt wheels I are connected with belt wheels in the corresponding Z-direction idler wheel brackets through the Z-direction lifting synchronous belt, the Z-axis motor bracket is fixedly arranged in the upper cover, and the Z-axis motor bracket is provided with the Z-direction lifting motor which is connected with the synchronous belt wheels II through the Z-direction motor synchronous belt; the Z-direction lifting synchronous belt is connected with four corners of the object placing table, and the object placing table is lifted by the Z-direction lifting mechanism.

Preferably, the object placing table 11 includes an X-axis fixing plate, an X-axis motor bracket, an X-axis motor, an X-axis driven wheel frame, an X-axis synchronous pulley, an X-direction driving synchronous belt, an X-axis track locking plate, a Y-axis motor bracket, a Y-direction driving synchronous belt, a Y-axis track, a linear bearing, a Y-axis bottom plate, a rotating pallet, a Y-axis track locking plate, a Y-axis driven wheel frame, and a Y-axis synchronous pulley;

The X-axis synchronous belt is characterized in that two sides above the X-axis fixing plate are respectively provided with an X-axis track locking plate, two X-axis tracks are arranged on the two X-axis track locking plates in a crossing manner, two linear bearings are respectively fixed on the X-axis tracks, a Y-axis bottom plate is fixed on the linear bearings through screws, one side of the X-axis fixing plate is provided with an X-axis motor bracket, the X-axis motor bracket is provided with an X-axis motor, the other side of the X-axis fixing plate is provided with an X-axis driven wheel frame, an X-axis motor output shaft and the X-axis driven wheel frame are respectively provided with an X-axis synchronous belt pulley, the two X-axis synchronous belt pulleys are connected through an X-direction driving synchronous belt, and the bottom surface of the Y-axis bottom plate is fixedly connected with the X-direction driving synchronous belt; the whole object placing table 11 moves along the X-axis direction by the mechanism;

Two sides above the Y-axis bottom plate are respectively provided with a Y-axis track locking plate, two Y-axis tracks are arranged on the two Y-axis track locking plates in a straddling way, two linear bearings are respectively fixed on the Y-axis tracks, a rotary supporting plate is fixed on the linear bearings through screws, one side of the Y-axis bottom plate is provided with a Y-axis motor bracket, the Y-axis motor bracket is provided with a Y-axis motor, the other side of the Y-axis bottom plate is provided with a Y-axis driven wheel frame, an output shaft of the Y-axis motor and the Y-axis driven wheel frame are respectively provided with a Y-axis synchronous belt pulley, the two Y-axis synchronous belt pulleys are connected through a Y-direction driving synchronous belt, and the bottom surface of the rotary supporting plate is fixedly connected with the Y-direction driving synchronous belt; the whole object placing table moves along the Y-axis direction through the mechanism;

A rotary platform is arranged on the rotary supporting plate;

the X-axis motor, the Y-axis motor and the rotary platform are respectively and electrically connected with the controller.

Preferably, the rotary platform comprises a rotary motor, a rotary shaft sleeve, a platform, a cylindrical gear I and a cylindrical gear II, wherein the rotary motor is longitudinally arranged at the bottom of the rotary supporting plate, the cylindrical gear I is positioned on the rotary supporting plate, the output part of the rotary motor is connected with the cylindrical gear I, the rotary shaft sleeve is arranged in the center of the rotary supporting plate, the cylindrical gear II is sleeved on the rotary shaft sleeve, the cylindrical gear II is meshed with the cylindrical gear I, the platform is fixedly arranged on the cylindrical gear II, and the rotary motor is electrically connected with the controller; the platform is rotated by this mechanism.

The controller is control equipment well known to those skilled in the art, such as a singlechip, a PLC controller and the like, each control button for controlling Z-axis lifting, X-axis moving, Y-axis moving and rotating is arranged on the control panel, and each button sends forward and reverse signals to the corresponding controlled motor through the controller so as to complete control, and is provided with a switch of a lamp and a camera.

The three-band point light source adopts the LED lamp panel with three excitation wavelengths, and has the advantages of low price, pure light source and long service life compared with the mercury lamp light source; the type of light source (395 nm, 514 nm, 527 and nm) is added, and the fluorescent protein such as GFP, YFP, RFP can be detected.

Preferably, the three-band point light source is an LED lamp panel with adjustable brightness, and the wavelengths are 395 nm, 514 nm, 527 nm; the brightness-adjustable LED lamp panel is equipment well known to a person skilled in the art, such as a dimmer is arranged, and under the condition that the required brightness for observation is ensured, the influence of autofluorescence on observation can be reduced, and the eyesight of the observer is also protected.

Preferably, the filter is a 509 nm, 527 nm or 588nm filter, which can properly filter the autofluorescence and excitation light of the observation material, is more suitable for observing the emitted light of a target wave band, and can reduce the injury of ultraviolet light to observers.

The utility model has the beneficial effects that:

1. The utility model integrally adopts an openable box body structure, and the observed object is closed after being placed in the box, so that the influence of natural light on the observed result can be greatly reduced; the three-band point light source can be used for detecting GFP, YFP, RFP and other fluorescent proteins, and meanwhile, the optical filter is replaced according to the corresponding light source type, so that the use is convenient, and the injury to observers is reduced; the multi-surface light source in the box body is beneficial to reducing the influence of local shadow on observation and reducing the color distortion degree;

2. The object placing table has the functions of Z-axis lifting, X-axis moving, Y-axis moving and rotating, wherein a Z-axis driving and synchronizing mechanism is arranged in the upper cover, and the structure is ingenious and does not occupy the observation space; and the X-axis movement, the Y-axis movement and the rotating mechanism are integrated on the object placing table, so that the structure is compact and the occupied space is small.

Drawings

FIG. 1 is a schematic diagram of a front view structure of the present utility model;

FIG. 2 is a schematic cross-sectional view of A-A of FIG. 1;

FIG. 3 is a schematic top view of the internal structure of the upper cover;

FIG. 4 is a schematic perspective view of a placement table;

FIG. 5 is a schematic perspective view of the platform with the platform removed;

In the figure: 1-control box, 2-control panel, 3-outer box, 4-split box door, 5-filter, 6-upper cover, 7-Z-direction idler wheel bracket, 8-controller, 9-slot, 10-side radiator, 11-object placing table, 1101-X-axis fixing plate, 1102-X-axis motor bracket, 1103-X-axis motor, 1104-X-axis driven wheel frame, 1105-synchronous pulley, 1106-X-direction driving synchronous belt, 1107-X-axis track, 1108-X-axis track locking plate, 1109-Y-axis motor, 1110-Y-axis motor bracket, 1111-rotating shaft sleeve, 1112-platform, 1113-cylindrical gear I, 1114-cylindrical gear II, 1115-Y drive synchronous belt, 1116-Y track, 1117-linear bearing, 1118-Y base plate, 1119-rotary support plate, 1120-Y track locking plate, 1121-Y driven wheel frame, 1122-Y synchronous belt wheel, 1123-rotary motor, 12-three-band point light source, 13-rear radiator, 14-Z lifting synchronous belt, 15-camera, 1601-synchronous belt wheel I, 1602-synchronous belt wheel II, 17-Z motor synchronous belt, 18-Z lifting motor, 19-Z motor bracket, 20-Z drive synchronous belt, 21-belt seat bearing and 22-Z driving shaft.

Detailed Description

The utility model is further described below with reference to the drawings and examples, but is not limited in any way, and any changes or substitutions based on the teachings of the utility model are intended to fall within the scope of the utility model.

Example 1

As shown in fig. 1-5, the observation device for detecting transgenic fluorescent protein in this embodiment includes a control box 1, a control panel 2, and an outer box 3, wherein a controller 8 is disposed in the control box 1, the control panel 2 is disposed outside the control box 1, the outer box 3 is disposed on the control box 1, a split box door 4 is disposed on the front of the outer box 3, a window is disposed on the split box door 4, a slot 9 is disposed in the window, an optical filter 5 is disposed in the slot, a side radiator 10 and a rear radiator 13 are disposed in the outer box 3, three-band point light sources 12 are uniformly disposed on the side radiator 10 and the rear radiator 13, a light scattering sheet is covered on the surface of the three-band point light sources 12, a camera 15 is disposed in the inner portion of the outer box 3, a Z-direction lifting mechanism is disposed in the outer box 3, the Z-direction lifting mechanism drives a storage table 11 to lift, and the controller 8 is electrically connected with the control panel 2, the three-point light sources 12, the camera 15 and the Z-direction lifting mechanism respectively; the three-band point light source 12 is an LED lamp panel with adjustable brightness and has the wavelengths 395 nm, 514 nm and 527 nm; the filter 5 is 509 nm, 527 nm or 588 nm;

The Z-direction lifting mechanism comprises a Z-direction idler wheel support 7, a Z-direction lifting synchronous belt 14, synchronous belt wheels I1601, synchronous belt wheels II 1602, a Z-direction motor synchronous belt 17, a Z-direction lifting motor 18, a Z-direction motor support 19, a Z-direction driving synchronous belt 20, a belt seat bearing 21 and a Z-direction driving shaft 22, wherein the Z-direction idler wheel support 7 is fixed at the bottom in the control box 1 through bolts, two pairs of belt seat bearings 21 are fixed in an upper cover 6 at the top of the outer box 3, each pair of belt seat bearings 21 is provided with a Z-direction driving shaft 22, two end surfaces of each Z-direction driving shaft 22 are respectively provided with a synchronous belt wheel I1601, the synchronous belt wheels I1601 corresponding to different Z-direction driving shafts 22 are connected through the Z-direction driving synchronous belt 20, the synchronous belt wheels I1601 are connected with the corresponding belt wheels in the Z-direction idler wheel support 7 through the Z-direction lifting synchronous belt 14, the Z-direction motor support 19 is fixed in the upper cover 6, the Z-direction lifting motor support 19 is provided with the Z-direction lifting motor 18, and the Z-direction lifting motor 18 is connected with the synchronous belt wheels II 1601 through the Z-direction motor synchronous belt 17; the Z-direction lifting synchronous belt 14 is connected with four corners of the object placing table 11;

The object placing table 11 includes an X-axis fixing plate 1101, an X-axis motor bracket 1102, an X-axis motor 1103, an X-axis driven wheel frame 1104, an X-axis synchronous pulley 1105, an X-direction driving synchronous belt 1106, an X-axis track 1107, an X-axis track locking plate 1108, a Y-axis motor 1109, a Y-axis motor bracket 1110, a Y-direction driving synchronous belt 1115, a Y-axis track 1116, a linear bearing 1117, a Y-axis bottom plate 1118, a rotating pallet 1119, a Y-axis track locking plate 1120, a Y-axis driven wheel frame 1121, and a Y-axis synchronous pulley 1122;

Two sides above the X-axis fixing plate 1101 are respectively provided with an X-axis track locking plate 1108, two X-axis tracks 1107 are arranged on the two X-axis track locking plates 1108 in a straddling manner, two linear bearings 1117 are respectively fixed on the X-axis tracks 1107, a Y-axis bottom plate 1118 is fixed on the linear bearings 1117 through screws, one side of the X-axis fixing plate 1101 is provided with an X-axis motor bracket 1102, the X-axis motor bracket 1102 is provided with an X-axis motor 1103, the other side of the X-axis fixing plate 1101 is provided with an X-axis driven wheel frame 1104, an output shaft of the X-axis motor 1103 and the X-axis driven wheel frame 1104 are respectively provided with an X-axis synchronous pulley 1105, the two X-axis synchronous pulleys 1105 are connected through an X-direction driving synchronous belt 1106, and the bottom surface of the Y-axis bottom plate 1118 is fixedly connected with the X-direction driving synchronous belt 1106;

Two sides above the Y-axis bottom plate 1118 are respectively provided with a Y-axis track locking plate 1120, two Y-axis tracks 1116 are arranged on the two Y-axis track locking plates 1120 in a straddling way, two linear bearings 1117 are respectively fixed on the Y-axis tracks 1116, a rotary supporting plate 1119 is fixed on the linear bearings 1117 through screws, one side of the Y-axis bottom plate 1118 is provided with a Y-axis motor bracket 1110, the Y-axis motor bracket 1110 is provided with a Y-axis motor 1109, the other side of the Y-axis bottom plate 1118 is provided with a Y-axis driven wheel frame 1121, the output shaft of the Y-axis motor 1109 and the Y-axis driven wheel frame 1121 are respectively provided with a Y-axis synchronous pulley 1122, the two Y-axis synchronous pulleys 1122 are connected through a Y-direction driving synchronous belt 1115, and the bottom surface of the rotary supporting plate 1119 is fixedly connected with the Y-direction driving synchronous belt 1115;

a rotating platform is mounted on the rotating pallet 1119; the X-axis motor 1103, the Y-axis motor 1109 and the rotating platform are respectively and electrically connected with the controller; the rotary platform comprises a rotary motor 1123, a rotary shaft sleeve 1111, a platform 1112, a cylindrical gear I1113 and a cylindrical gear II 1114, wherein the rotary motor 1123 is longitudinally arranged at the bottom of the rotary supporting plate 1119, the cylindrical gear I1113 is positioned above the rotary supporting plate 1119, the output part of the rotary motor 1123 is connected with the cylindrical gear I1113, the rotary shaft sleeve 1111 is arranged in the center of the rotary supporting plate 1119, the cylindrical gear II 1114 is sleeved on the rotary shaft sleeve 1111, the cylindrical gear II 1114 is meshed with the cylindrical gear I1113, the platform 1112 is fixedly arranged on the cylindrical gear II 1114, and the rotary motor 1123 is electrically connected with a controller; the platform is rotated by this mechanism.

The working principle and the working process of the utility model are as follows: a light source with a proper wave band and an optical filter 5 are selected according to the requirement; opening the split type box door 4, placing the transgenic fluorescent protein sample to be observed on the platform 1112, and closing the split type box door 4; the Z-direction lifting motor 18 is started, and the Z-direction lifting motor 18 drives the Z-direction driving shaft 22 to rotate, so that the Z-direction driving synchronous belt 20 synchronously moves, and the object placing table 11 is lifted to a proper position convenient for observation; then, the X-axis motor 1103 and the Y-axis motor 1109 are started as required to drive the corresponding driving synchronous belt to move, so that the X-axis position and the Y-axis position of the platform 1112 move; in the process of observing the sample in the box through the optical filter 5, a rotating motor 1123 can be started, and the platform 1112 is driven to rotate through the cylindrical gear I1113 and the cylindrical gear II 1114, so that the multi-angle observation is convenient; in observation, the camera 15 can be started to shoot and record.

Claims (6)

1. The utility model provides an observation device for transgenic fluorescent protein detects, includes control box (1), control panel (2), outer box (3), its characterized in that be equipped with controller (8) in control box (1), control panel (2) are located outside control box (1), outer box (3) are located on control box (1), and outer box (3) openly are equipped with split type chamber door (4), have offered the window on split type chamber door (4), and the window is equipped with slot (9), has placed light filter (5) in the slot, and outer box (3) inside is equipped with side radiator (10), back radiator (13), and side radiator (10), back radiator (13) are gone up and have evenly been laid three wave band point light sources (12) respectively, and three wave band point light sources (12) surface covering have scattered light sheet, and outer box (3) are inside to be equipped with camera (15), are equipped with Z to elevating system in outer box (3), and Z is to elevating system drive and put thing platform (11) and go up and down, controller (8) are connected with control panel (2), three wave point light sources (12), Z to elevating system.

2. The observation device for detecting the transgenic fluorescent protein according to claim 1, wherein the Z-direction lifting mechanism comprises a Z-direction idler bracket (7), a Z-direction lifting synchronous belt (14), a synchronous pulley I (1601), a synchronous pulley II (1602), a Z-direction motor synchronous belt (17), a Z-direction lifting motor (18), a Z-direction motor bracket (19), a Z-direction driving synchronous belt (20), a belt seat bearing (21) and a Z-direction driving shaft (22), the bottom in the control box (1) is fixedly provided with the Z-direction idler bracket (7) through bolts, two pairs of belt seat bearings (21) are fixedly arranged in an upper cover (6) at the top of the outer box (3), each pair of belt seat bearings (21) is provided with a Z-direction driving shaft (22), two end surfaces of the Z-direction driving shaft (22) are respectively provided with a synchronous pulley I (1601), the synchronous pulleys I (1601) corresponding to the different Z-direction driving shafts (22) are connected through the Z-direction driving synchronous belt (20), the synchronous pulleys I (1601) are connected with the corresponding belt pulleys in the Z-direction idler bracket (7) through the Z-direction lifting synchronous belt motor (14), the Z-direction driving motor (19) is fixedly arranged in the Z-direction bracket (19), the Z-direction lifting motor (18) is connected with the synchronous pulley II (1602) through the Z-direction motor synchronous belt (17), and the Z-direction lifting synchronous belt (14) is connected with four corners of the object placing table (11).

3. The observation device for detecting the transgenic fluorescent protein according to claim 1, wherein the object placing table (11) comprises an X-axis fixing plate (1101), an X-axis motor bracket (1102), an X-axis motor (1103), an X-axis driven wheel frame (1104), an X-axis synchronous pulley (1105), an X-axis driving synchronous belt (1106), an X-axis rail (1107), an X-axis rail locking plate (1108), a Y-axis motor (1109), a Y-axis motor bracket (1110), a Y-axis driving synchronous belt (1115), a Y-axis rail (1116), a linear bearing (1117), a Y-axis bottom plate (1118), a rotary supporting plate (1119), a Y-axis rail locking plate (1120), a Y-axis driven wheel frame (1121) and a Y-axis synchronous pulley (1122);

Two sides above the X-axis fixing plate (1101) are respectively provided with an X-axis track locking plate (1108), two X-axis tracks (1107) are arranged on the two X-axis track locking plates (1108) in a straddling manner, two linear bearings (1117) are respectively fixed on the X-axis tracks (1107), a Y-axis bottom plate (1118) is fixed on the linear bearings (1117) through screws, one side of the X-axis fixing plate (1101) is provided with an X-axis motor bracket (1102), the X-axis motor bracket (1102) is provided with an X-axis motor (1103), the other side of the X-axis fixing plate (1101) is provided with an X-axis driven wheel frame (1104), an output shaft of the X-axis motor (1103) and the X-axis driven wheel frame (1104) are respectively provided with an X-axis synchronous pulley (1105), the two X-axis synchronous pulleys (1105) are connected through an X-axis driving synchronous belt (1106), and the bottom surface of the Y-axis bottom plate (1118) is fixedly connected with the X-axis driving synchronous belt (1106);

Two sides above the Y-axis bottom plate (1118) are respectively provided with a Y-axis track locking plate (1120), two Y-axis tracks (1116) are arranged on the two Y-axis track locking plates (1120) in a straddling way, two linear bearings (1117) are respectively fixed on the Y-axis tracks (1116), a rotary supporting plate (1119) is fixed on the linear bearings (1117) through screws, one side of the Y-axis bottom plate (1118) is provided with a Y-axis motor bracket (1110), the Y-axis motor bracket (1110) is provided with a Y-axis motor (1109), the other side of the Y-axis bottom plate (1118) is provided with a Y-axis driven wheel frame (1121), the output shaft of the Y-axis motor (1109) and the Y-axis driven wheel frame (1121) are respectively provided with a Y-axis synchronous pulley (1122), the two Y-axis synchronous pulleys (1122) are connected through a Y-direction driving synchronous belt (1115), and the bottom surface of the rotary supporting plate (1119) is fixedly connected with the Y-direction driving synchronous belt (1115);

a rotary platform is arranged on the rotary supporting plate (1119);

The X-axis motor (1103), the Y-axis motor (1109) and the rotary platform are respectively and electrically connected with the controller.

4. The observation device for detecting the transgenic fluorescent protein according to claim 3, wherein the rotary platform comprises a rotary motor (1123), a rotary shaft sleeve (1111), a platform (1112), a cylindrical gear I (1113) and a cylindrical gear II (1114), the rotary motor (1123) is longitudinally arranged at the bottom of the rotary supporting plate (1119), the cylindrical gear I (1113) is arranged above the rotary supporting plate (1119), an output part of the rotary motor (1123) is connected with the cylindrical gear I (1113), the rotary shaft sleeve (1111) is arranged in the center of the rotary supporting plate (1119), the cylindrical gear II (1114) is sleeved on the rotary shaft sleeve (1111), the cylindrical gear II (1114) is meshed with the cylindrical gear I (1113), the platform (1112) is fixedly arranged on the cylindrical gear II (1114), and the rotary motor (1123) is electrically connected with the controller.

5. The observation device for detecting the transgenic fluorescent protein according to claim 1, wherein the three-band point light source (12) is an LED lamp panel with adjustable brightness, and the wavelengths are 395 nm, 514 nm and 527 nm.

6. The viewing device for transgenic fluorescent protein detection according to claim 1, characterized in that the filter (5) is a 509 nm, 527 nm or 588nm filter.