CN221810384U - A bionic organ chip of anterior chamber tissue - Google Patents

Abstract

The utility model relates to the technical field of human disease scientific research detection, and discloses an anterior chamber tissue bionic organ chip, including a bottom plate and an upper cover plate, the upper cover plate is fixedly connected to the top of the bottom plate, the bottom plate is provided with a bionic area inside, the upper surface of the bottom plate is provided with a microfluidic flow channel, and the upper surface of the bionic area is provided with a detection area; the development of the anterior chamber tissue bionic organ chip can simulate and construct a three-dimensional human organ model in vitro, has physiological functions close to the human level, and can accurately control multiple system parameters at the same time, and has broad development prospects in the fields of disease simulation, new drug research and development, and precision medicine. The research and development of this organ chip can not only avoid ethical issues, but also show great advantages in accuracy, throughput and cost. Therefore, both economic and social benefits have good prospects.

Description

A bionic organ chip of anterior chamber tissue

Technical Field

The utility model relates to the technical field of human disease scientific research detection, in particular to an anterior chamber tissue bionic organ chip.

Background Art

Microfluidics chip technology integrates the basic operating units of sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes onto a micrometer-scale chip to automatically complete the entire analysis process. Microfluidics is a technology that uses microchannels to process or manipulate tiny fluids. It is widely used in various test-related fields such as cell culture, cell stimulation, cell analysis, nucleic acid extraction, nucleic acid amplification, biochemical testing, immunoassay, and environmental monitoring.

Microfluidics are tiny devices designed to contain and manipulate small amounts of fluid (measured in microliters) in channels that are one-hundredth the width of a human hair. The microfluidic device designed in the current study is divided into multiple chambers to achieve the separation and manipulation of different substances within the device. Multi-chamber microfluidic devices provide a controllable environment to study various biological and chemical phenomena. This newly developed device can co-culture different cells and simulate the activities, mechanisms and physiological responses of the human eye, etc.

In the past, most studies on the physiological structure of the eye and the pathological mechanisms of diseases have used in vitro cell culture or animal models. However, since the in vitro cell environment is not completely consistent with that in vivo and there are large differences between different species, these studies are subject to certain limitations in clinical translation.

Utility Model Content

1. Technical issues to be resolved

In view of the shortcomings of the prior art, the utility model provides a bionic organ chip of anterior chamber tissue, which is designed to simulate the anterior chamber tissue of the eye in a microfluidic chip, thereby providing an effective research platform for studying and revealing its biological nature. The bionic material can induce eye cells to achieve a specific spatial arrangement, provide fluid conditions, mechanical stimulation and chemical stimulation similar to the in vivo environment, thereby simulating a more realistic physiological and even pathological environment in vitro, solving the problem that the in vitro cell environment is not completely consistent with the in vivo environment and there are large differences between different species.

(II) Technical solution

To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a bionic organ chip of anterior chamber tissue, comprising a bottom plate and an upper cover plate, wherein the upper cover plate is fixedly connected to the top of the bottom plate, a bionic area is provided inside the bottom plate, a microfluidic channel is provided on the upper surface of the bottom plate, and a detection area is provided on the upper surface of the bionic area.

Preferably, the bottom plate is made of PS material with a thickness of 30mm-40mm, and the upper cover plate is made of PMMA or COC material with a thickness of 20mm-30mm and good light transmittance.

Preferably, the upper cover plate comprises: a plate body, and the upper surface of the plate body is provided with a drug injection inlet, a liquid recovery outlet, a tear injection inlet, a tear injection outlet, an aqueous humor injection inlet and an aqueous humor injection outlet.

Preferably, the bionic area comprises:

A corneal biomimetic area is provided on the upper surface of the bottom plate;

The anterior chamber bionic area is opened on the upper surface of the bottom plate.

Preferably, the microfluidic channel comprises:

A drug injection channel, one end of which is connected to the drug injection inlet, and the other end of which is connected to the bionic area;

A liquid recovery channel, one end of which is connected to the bionic area, and the other end of which is connected to the liquid recovery outlet;

A tear injection inlet flow channel, one end of which is connected to the tear injection inlet, and the other end of which is connected to the bionic area;

A tear injection outlet flow channel, one end of which is connected to the tear injection outlet, and the other end of which is connected to the bionic area;

An aqueous humor injection inlet flow channel, one end of which is connected to the aqueous humor injection inlet, and the other end of which is connected to the bionic area;

An aqueous humor injection outlet flow channel has one end connected to the aqueous humor injection outlet and the other end connected to the bionic area.

Preferably, the detection area comprises:

A detection electrode is arranged between the corneal biomimetic area and the anterior chamber biomimetic area;

The electrode wiring is arranged at the wiring terminal of the detection electrode.

(III) Beneficial effects

Compared with the prior art, the utility model provides an anterior chamber tissue bionic organ chip, which has the following beneficial effects:

The development of the anterior chamber tissue bionic organ chip can simulate and construct a three-dimensional human organ model in vitro, with physiological functions close to the human level, and can accurately control multiple system parameters. It has broad development prospects in the fields of disease simulation, new drug development, and precision medicine. The development of this organ chip can not only avoid ethical issues, but also show great advantages in accuracy, throughput, and cost. Therefore, both economic and social benefits have good prospects.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 is a schematic diagram of the structure of the utility model;

Figure 2 is a schematic diagram of the structure of the upper cover plate in the utility model;

FIG3 is a cross-sectional view of the bottom layer plate of the utility model;

FIG. 4 is a diagram showing the connection relationship between the bionic area, the microfluidic channel and the detection area in the present invention.

In the figure:

10. Bottom plate;

20. Upper cover plate; 21. Plate body; 22. Drug injection inlet; 23. Liquid recovery outlet; 24. Tear injection inlet; 25. Tear injection outlet; 26. Aqueous humor injection inlet; 27. Aqueous humor injection outlet;

30. Bionic area; 31. Corneal bionic area; 32. Anterior chamber bionic area;

40. Microfluidic channel; 41. Drug injection channel; 42. Liquid recovery channel; 43. Tear injection inlet channel; 44. Tear injection outlet channel; 45. Aqueous humor injection inlet channel; 46. Aqueous humor injection outlet channel;

50. Detection area; 51. Detection electrode; 52. Electrode wiring.

DETAILED DESCRIPTION

The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

Embodiment 1

A bionic organ chip of anterior chamber tissue comprises a bottom plate 10 and an upper cover plate 20. The upper cover plate 20 is fixedly connected to the top of the bottom plate 10. A bionic area 30 is provided inside the bottom plate 10. A microfluidic channel 40 is provided on the upper surface of the bottom plate 10. A detection area 50 is provided on the upper surface of the bionic area 30.

In this embodiment, specifically, the bottom plate 10 is made of PS material with a thickness of 30 mm-40 mm, and the upper cover plate 20 is made of PMMA or COC material with a thickness of 20 mm-30 mm and good light transmittance and colorless.

In this embodiment, specifically, the upper cover plate 20 includes: a plate body 21, and the upper surface of the plate body 21 is provided with a drug injection inlet 22, a liquid recovery outlet 23, a tear injection inlet 24, a tear injection outlet 25, an aqueous humor injection inlet 26 and an aqueous humor injection outlet 27.

In this embodiment, specifically, the bionic area 30 includes:

The corneal biomimetic area 31 is provided on the upper surface of the bottom plate 10;

The anterior chamber bionic area 32 is provided on the upper surface of the bottom plate 10 .

In this embodiment, specifically, the microfluidic channel 40 includes:

A drug injection channel 41, one end of which is connected to the drug injection inlet 22, and the other end of which is connected to the bionic area 30;

A liquid recovery channel 42, one end of which is connected to the bionic area 30, and the other end of which is connected to the liquid recovery outlet 23;

A tear injection inlet channel 43, one end of which is connected to the tear injection inlet 24, and the other end of which is connected to the bionic area 30;

A tear injection outlet channel 44, one end of which is connected to the tear injection outlet 25, and the other end of which is connected to the bionic area 30;

An aqueous humor injection inlet flow channel 45, one end of which is connected to the aqueous humor injection inlet 26, and the other end of which is connected to the bionic area 30;

One end of the aqueous humor injection outlet channel 46 is connected to the aqueous humor injection outlet 27 , and the other end is connected to the bionic area 30 .

In this embodiment, specifically, the detection area 50 includes:

The detection electrode 51 is arranged between the corneal biomimetic area 31 and the anterior chamber biomimetic area 32;

The electrode connection 52 is provided at the connection end of the detection electrode 51 .

In this embodiment, the following steps are included:

Step 1: Distinguish the various functional areas and requirements of the chip through relevant designs;

Step 2: Prepare various components of the chip;

Step 3: Modify the relevant area in the area of the corresponding chip;

Step 4: Add the sample to the microfluidic chip and test the results.

In this embodiment, compared with traditional tissue and organ culture, the bionic chip has the following advantages:

Small sample volume, saving raw materials and reagents;

Accurately controlling the rate of aqueous humor metabolism can better reflect the diffusion of drugs, etc.

Referring to Fig. 1-4, the upper cover plate 20: the upper cover plate 20 is colorless and transparent, with good light transmittance; the thickness is 20mm-30mm; the material is preferably PMMA or COC; each area of the upper cover plate has a circular sample injection hole;

Bottom plate 10: The bottom layer is PS or other materials, with a thickness of 30 mm -40 mm; the bottom surface is engraved with a microfluidic channel 40, and the functions are as follows:

Drug injection channel 41: simulates dripping of eye drops through an external injection pump and maintains the concentration to a therapeutic level;

The tear injection inlet channel 43 and the tear injection outlet channel 44 are used to constantly simulate the production of tears on the ocular surface through an external injection pump;

Corneal bionic area 31 and anterior chamber bionic area 32: a layer of corneal cells is cultured on a polymer semipermeable membrane by in-situ culture technology, mainly simulating the diffusion of drugs into the anterior chamber of the eye;

Detection area 50: has a pre-buried gold film (detection electrode 51 and electrode wiring 52), and through the modification technology, the diffusion concentration of the drug can be monitored in real time;

Aqueous humor injection inlet channel 45 and aqueous humor injection outlet channel 46: through an external injection pump, the production of aqueous humor is constantly simulated;

Liquid recovery channel 42: recovers some required aqueous humor simulation solution.

Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims (3)

1. The anterior chamber tissue bionic organ chip comprises a bottom plate (10) and an upper cover plate (20), wherein the upper cover plate (20) comprises: the utility model provides a plate body (21), drug injection entry (22), liquid recovery export (23), tear injection entry (24), tear injection export (25), aqueous humor injection entry (26) and aqueous humor injection export (27) have been seted up to the upper surface of plate body (21), upper cover plate (20) fixed connection in the top of bottom plate (10), its characterized in that: the inside of bottom plate (10) is equipped with bionical district (30), bionical district (30) include: the cornea bionic area (31) is arranged on the upper surface of the bottom plate (10); the anterior chamber bionic area (32) is arranged on the upper surface of the bottom plate (10), a microfluidic flow channel (40) is arranged on the upper surface of the bottom plate (10), a detection area (50) is arranged on the upper surface of the bionic area (30), and the detection area (50) comprises: a detection electrode (51) arranged between the cornea bionic region (31) and the anterior chamber bionic region (32); and an electrode connection (52) provided at a terminal of the detection electrode (51).

2. The anterior chamber tissue biomimetic organ-chip of claim 1, wherein: the bottom plate (10) is made of PS material with the thickness of 30-40 mm, and the upper cover plate (20) is made of PMMA or COC material with the thickness of 20-30 mm and good colorless and transparent light transmittance.

3. The anterior chamber tissue biomimetic organ-chip of claim 1, wherein: the microfluidic flow channel (40) comprises:

A liquid medicine injection flow passage (41), one end of which is connected with the medicine injection inlet (22) and the other end of which is connected with the bionic area (30);

A liquid recovery flow channel (42), one end of which is connected with the bionic area (30) and the other end of which is connected with the liquid recovery outlet (23);

A tear injection inlet flow channel (43) with one end connected to the tear injection inlet (24) and the other end connected to the bionic region (30);

a tear injection outlet flow passage (44) with one end connected to the tear injection outlet (25) and the other end connected to the bionic region (30);

An aqueous humor injection inlet runner (45), one end of which is connected with the aqueous humor injection inlet (26) and the other end of which is connected with the bionic area (30);

Fang Shuizhu jet outlet flow channels (46), one end of which is connected with the aqueous humor jet outlet (27), and the other end of which is connected with the bionic area (30).