CN221770394U - A device for alveolar lavage of rats and mice - Google Patents

Abstract

The utility model discloses a large and small mouse alveolus lavage device, which comprises: the fixing device is used for fixing the experimental rat or mouse; the irrigation and aspiration device is used for carrying out alveolar lavage on the experimental rat or mouse and collecting biological samples; the irrigation and suction device comprises an irrigation and suction device, a connecting hose and an air pipe plug; one end of the connecting hose is connected with a filling port of the filling and sucking device; the other end of the connecting hose is connected with the tracheal cannula head; the utility model can solve the problems of leakage and red blood cell pollution caused by the need of dissection, difficult fixation and easy puncture of lungs of the big and small mice in the traditional alveolus lavage operation of the big and small mice, low utilization rate of experimental samples and higher operation difficulty for novice, and has convenient operation and high efficiency.

Description

Alveolar lavage device for rats and mice

Technical Field

The utility model relates to the technical field of experimental animal sample collection, in particular to a large and small mouse alveolus lavage device.

Background

The enhancement of the research on respiratory diseases has important significance for guaranteeing the health of people in China.

As the most commonly used experimental animals, the large and small mice can effectively simulate the symptoms of human lung diseases by a modeling means, and alveolar lavage and biological sample collection of the model mice are important links for researching respiratory diseases.

When large and small mice are used for pulmonary disease related research, the traditional method often needs to perform alveolar lavage of the large and small mice to collect biological samples so as to detect related indexes; existing alveolar lavage methods often require the animal to be sacrificed and dissected, and the needle inserted into the mouse's trachea and fixed prior to alveolar lavage.

The traditional operation method has complicated operation and low success rate for novice experimenters, is easy to generate the problems of unstable fixation, difficult extraction of liquid, easy pollution of liquid and the like, and seriously influences the progress of related disease research.

Disclosure of utility model

The utility model aims to provide a large and small mouse alveolus lavage device which is used for solving the problems in the background technology.

In order to achieve the above object, the present utility model provides the following solutions:

a large, mouse alveolar lavage device comprising:

the fixing device is used for fixing the experimental rat or mouse;

The irrigation and aspiration device is used for carrying out alveolar lavage on the experimental rat or mouse and collecting biological samples;

the irrigation and suction device comprises an irrigation and suction device, a connecting hose and an air pipe plug;

one end of the connecting hose is connected with a filling port of the filling and sucking device;

The other end of the connecting hose is connected with the tracheal cannula head.

Further, the fixing device comprises a fixing plate and a head fixing rod;

The head fixing rod is detachably arranged on the front side wall of the fixing plate;

The front side wall of the fixing plate is correspondingly provided with two groups of four-limb fixing assemblies, the two groups of four-limb fixing assemblies are respectively arranged on two sides of the head fixing rod, and each group of four-limb fixing assemblies comprises a plurality of four-limb fixing buckles which are vertically arranged.

Further, the fixing device further comprises a fixing seat, and the fixing plate is vertically arranged on the fixing seat;

The head fixing rod is U-shaped.

Further, a strip-shaped groove is formed in the front side wall of the fixing plate, and the groove is located between the two groups of four-limb fixing assemblies.

Further, a tracheal sealing plug is sleeved on the side wall of the tracheal cannula head.

Further, the tracheal cannula head is a curved metal lavage needle.

Further, the air tube sealing plug is a rubber plug with a hole in the middle.

Compared with the prior art, the utility model has the beneficial effects that:

The utility model can effectively solve the problems of leakage and red blood cell pollution caused by the fact that the traditional alveolar lavage operation of the big and small mice needs to be performed on the big and small mice, is difficult to fix and easy to puncture the lung, has low utilization rate of experimental samples and higher operation difficulty for novice, and has convenient operation and high efficiency.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of a fixing device according to an embodiment of the present utility model;

fig. 2 is a schematic structural diagram of a sucking device according to an embodiment of the present utility model.

Reference numerals illustrate:

1. A fixing plate; 2. a head fixing rod; 3. fixing buckles for four limbs; 4. a groove; 5. a irrigator; 6. a connecting hose; 7. a tracheal cannula head; 8. the trachea is closed tightly.

Detailed Description

The following describes in further detail the embodiments of the present utility model with reference to the drawings and examples. The following examples are illustrative of the utility model and are not intended to limit the scope of the utility model.

In the description of the present application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.

The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.

The present utility model will be described in further detail with reference to the accompanying drawings for a better understanding of the objects, structures and functions of the present utility model.

Referring to fig. 1-2, a large and small mouse alveolar lavage device according to an embodiment of the present application is described, including:

the fixing device is used for fixing the experimental rat or mouse;

And the lavage device is used for performing alveolar lavage on the experimental rat or mouse and collecting a biological sample.

Specifically, as shown in fig. 1, the fixing device includes a fixing plate 1 and a head fixing rod 2;

The head fixing rod 2 is detachably mounted on the front side wall of the fixing plate 1;

the front side wall of the fixing plate 1 is correspondingly provided with two groups of four-limb fixing assemblies, the two groups of four-limb fixing assemblies are respectively arranged on two sides of the head fixing rod 2, and each group of four-limb fixing assemblies comprises a plurality of four-limb fixing buckles 3 which are vertically arranged.

It should be noted that, the fixing device further includes a fixing seat, and the fixing plate 1 is vertically disposed on the fixing seat, so that the fixing plate 1 can be ensured to be in a vertical state.

It should be noted that, the head fixing rod 2 is U-shaped, so as to be convenient for fixing the adaptation rat or mouse.

In addition, offer rectangular recess 4 on the preceding lateral wall of fixed plate 1, recess 4 is located between two sets of four limbs fixed subassembly, can be convenient for hold rat or mouse.

Specifically, after the rat or the mouse is anesthetized or killed, the mouth of the rat or the mouse is opened, the incisors of the rat or the mouse are hung on the head fixing rod 2, and the four limbs of the rat or the mouse are fixed by the four limbs fixing buckles 3 at proper positions according to the size of the rat or the mouse, so that the rat or the mouse is more convenient, quicker and firmer to fix.

Specifically, as shown in fig. 2, the irrigation and aspiration device includes an irrigation and aspiration device 5, a connection hose 6, and a tracheal cannula head 7;

one end of the connecting hose 6 is connected with a filling port of the filling and sucking device 5; the other end of the connecting hose 6 is connected with a tracheal cannula head 7;

the side wall of the tracheal cannula head 7 is sleeved with a tracheal sealing plug 8.

It should be noted that, the syringe 5 may be a 1mL or 2mL syringe, the length of the connection hose 6 is about 5cm, the tracheal cannula head 7 may be a curved metal gastric lavage needle, the tracheal sealing plug 8 is a rubber plug with a hole in the middle, and the tracheal cannula head 7 is passed through the hole and adjusted to a proper position.

Specifically, the irrigation and aspiration device 5 is matched with the connecting hose 6 and the tracheal cannula head 7, the tracheal cannula head 7 can be inserted into a rat or mouse trachea, under the connecting action of the connecting hose 6, the irrigation and aspiration device 5 is used for injection and aspiration, and the tracheal sealing plug 8 can ensure the air tightness in the irrigation and aspiration process.

Specifically, the method for using the large and small mouse alveolus lavage device is as follows:

S1, anaesthetizing a rat or a mouse, opening the mouth of the rat or the mouse, hanging the incisors of the rat or the mouse on a head fixing rod 2, and selecting an extremity fixing buckle 3 at a proper position according to the size of the rat or the mouse to fix the extremities of the rat or the mouse;

S2, inserting the tracheal cannula head 7 into a rat or mouse trachea, and ensuring air tightness through a tracheal sealing plug 8;

S3, pushing the lavage device 5, and pushing lavage liquid in the lavage device 5 into the lung of the rat or the mouse;

s4, pulling the perfusion and suction device 5 to pump out alveolar liquid;

s5, repeatedly performing the steps of S3 and S4, and collecting alveolar lavage fluid;

s6, taking out the tracheal cannula head 7 and putting the rat or the mouse back.

The principles and embodiments of the present utility model have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present utility model and the core ideas thereof; also, it is within the scope of the present utility model to be modified by those of ordinary skill in the art in light of the present teachings. In summary, the present description should not be construed as limiting the utility model.

Claims (7)

1. A large and small mouse alveolar lavage device, comprising:

the fixing device is used for fixing the experimental rat or mouse;

The irrigation and aspiration device is used for carrying out alveolar lavage on the experimental rat or mouse and collecting biological samples;

the irrigation and suction device comprises an irrigation and suction device, a connecting hose and an air pipe plug;

one end of the connecting hose is connected with a filling port of the filling and sucking device;

The other end of the connecting hose is connected with the tracheal cannula head.

2. The alveolar lavage device of claim 1 wherein said fixation device comprises a fixation plate and a head fixation rod;

The head fixing rod is detachably arranged on the front side wall of the fixing plate;

The front side wall of the fixing plate is correspondingly provided with two groups of four-limb fixing assemblies, the two groups of four-limb fixing assemblies are respectively arranged on two sides of the head fixing rod, and each group of four-limb fixing assemblies comprises a plurality of four-limb fixing buckles which are vertically arranged.

3. The alveolar lavage device of claim 2 wherein said fixation device further comprises a fixation base, said fixation plate being vertically disposed on said fixation base;

The head fixing rod is U-shaped.

4. The device of claim 2, wherein the front side wall of the fixing plate is provided with an elongated groove, and the groove is located between the two groups of limb fixing components.

5. The alveolar lavage device for large and small mice according to claim 1, wherein a tracheal sealing plug is sleeved on the side wall of the tracheal cannula head.

6. A large and small mouse alveolar lavage device as claimed in claim 1 wherein said tracheal cannula head is a curved metal lavage needle.

7. The alveolar lavage device of claim 5 wherein said tracheal sealing plug is a rubber plug with a hole in the middle.