CN221566091U - Intelligent cell pipetting workstation - Google Patents

Abstract

The utility model discloses an intelligent cell pipetting workstation which comprises an upper operation station, a centrifugal component and a feeding component, wherein the feeding component can receive a feeding culture tube, and the centrifugal component can centrifuge cell sap; the upper operation station comprises a pipetting component, a grabbing component and an operation table, wherein the pipetting component can absorb cell liquid into the centrifuge tube, the grabbing component can clamp the feeding culture tube on the feeding component to enter the upper operation station and be placed on the operation table, and the pipetting component can absorb culture liquid in the feeding culture tube to enter the centrifuge tube. The beneficial effects of the utility model are as follows: the whole machine has a more intelligent cell preparation process, replaces manual operation, and greatly improves accuracy and working efficiency; the built-in manipulator is matched with the sliding clamping piece, so that quick cell preparation is realized, and the standard requirement is met; the batch type whole-plate uncapping mechanism realizes the uncapping action of the quick whole-plate type sample tube by matching the sample box supporting plate and the manipulator, and greatly improves the efficiency of manually opening the upper cover of the sample tube.

Description

Intelligent cell pipetting workstation

Technical Field

The utility model relates to the technical field of bioengineering, in particular to an intelligent cell pipetting workstation.

Background

Along with the development of science and technology, bioengineering has higher standard requirements for cell culture work, and the high consistency and mass culture of cells are met.

The traditional cell culture equipment has low automation degree, such as cell culture bottle feeding and placing, cell culture by using a cell culture box, cell culture bottle labeling and other operations are all needed to be completed manually, and the corresponding working equipment is distributed at different positions, so that the working efficiency is low, and the labor intensity of staff is high; in the cell culture work, a plurality of culture mediums are required to be added into a cell culture bottle, and different culture mediums are required to be used in different culture processes, so that strict requirements are imposed on operators and operation processes, the operators should be well known about the use amount of the culture mediums required to be used in each process, and the quality and efficiency of the cell culture are seriously influenced because different poles of the experience and the manipulation of the operators easily cause the cell culture work to obtain extremely different results; the whole cell culture process needs to use various devices, needs a large floor area, and lacks automatic connection among various devices, so that operators are required to consume a large amount of time and heart blood, and the requirements of higher-efficiency cell culture cannot be met, and an intelligent cell pipetting workstation is urgently needed.

Disclosure of Invention

This section is intended to outline some aspects of embodiments of the utility model and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the utility model and in the title of the utility model, which may not be used to limit the scope of the utility model.

The present utility model has been made in view of the above-mentioned or existing problems occurring in the prior art.

Therefore, the utility model aims to provide an intelligent cell pipetting workstation which can be used for standardization and intelligent cell preparation, does not need manual operation and improves the quality and efficiency of cell culture.

In order to solve the technical problems, the utility model provides the following technical scheme: an intelligent cell pipetting workstation comprises an upper operation station, a centrifugal component and a feeding component,

The upper operation station is arranged above the centrifugal component and the feeding component,

The feeding component can receive the feeding culture tube, and the centrifugal component can centrifuge the cell sap;

The upper operation station comprises a pipetting component, a grabbing component and an operation table,

A centrifuge tube can be placed on the operating table, a pipetting component can absorb cell liquid into the centrifuge tube, a grabbing component can clamp a feeding culture tube on a feeding component to enter an upper operating station and be placed on the operating table,

The pipetting component can suck the culture solution in the feeding culture tube to enter the centrifuge tube;

The grabbing component can grab the centrifugal tube to enter the centrifugal component.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a sample box storage table assembly, a centrifuge tube storage table assembly, a culture solution storage table assembly and a culture bottle storage table assembly;

At least one group of centrifuge tubes are stored on the centrifuge tube storage table assembly, at least one group of freezing storage tubes are stored on the sample box storage table assembly, at least one group of feeding culture tubes are stored on the culture solution storage table assembly, and at least one group of culture bottles are stored on the culture bottle storage table assembly;

The sample box storage table assembly, the centrifuge tube storage table assembly, the culture solution storage table assembly and the culture bottle storage table assembly can be arranged in a autorotation or non-rotation fixed mode around the axis of the culture bottle storage table assembly;

The pipetting component can absorb the cell liquid in the freezing tube to enter the centrifuge tube and the culture bottle, and the pipetting component can absorb the complete culture medium in the feeding culture tube to enter the culture bottle.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the sample box storage table assembly comprises a sample box driving piece, a sample box supporting plate rotating shaft and a sample box;

The sample box driving piece drives the sample box supporting plate to rotate through the sample box supporting plate rotating shaft, at least one group of sample box position grooves are formed in the sample box supporting plate, sample boxes can be stored in the sample box position grooves, and freezing storage tubes can be stored in the sample boxes.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the centrifuge tube storage table assembly comprises a centrifuge tube turntable driving piece, a centrifuge tube turntable, an upper storage rack, a lower fixing seat and a centrifuge tube fixing piece;

the centrifuging tube carousel driving piece is connected with the centrifuging tube carousel, is provided with at least a set of storage rack and lower fixing base on the centrifuging tube carousel, and the centrifuging tube can be placed in last storage rack and lower fixing base, and the centrifuging tube mounting can be fixed the lower extreme of placing the centrifuging tube in lower fixing base.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the culture solution storage table assembly comprises a culture solution support plate, a culture solution support plate driving piece and a storage bottle hole site;

The culture solution supporting plate driving piece drives the culture solution supporting plate to rotate, at least one group of storage bottle hole sites are arranged on the culture solution supporting plate, and feeding culture tubes can be placed in the storage bottle hole sites.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the culture bottle storage table assembly comprises a culture bottle driving piece, a culture bottle supporting plate and a culture bottle storage groove;

The culture bottle driving piece is connected with a culture bottle supporting plate, at least one group of culture bottle storage grooves are formed in the culture bottle supporting plate, and culture bottles can be stored in the culture bottle storage grooves; the culture bottle driving piece can drive the culture bottle supporting plate to rotate.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the grabbing component comprises a robot arm piece and a sliding clamping piece;

The feeding culture tube on the feeding assembly can be grabbed by the manipulator piece, enters the upper operation station and is placed on the culture solution storage table assembly, and the sliding clamping piece can grab the grabbing centrifuge tube to enter the centrifugal assembly for centrifugation.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the robot arm piece comprises a robot arm;

The gripper head supporting frame is arranged at one side of the manipulator;

the first hand quick-change head, the second hand quick-change head and the third hand quick-change head are arranged on the hand-grip support frame.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a cover opening assembly, and the cover opening assembly can integrally open the freezing storage tube in the sample box storage table assembly.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the cover opening assembly comprises a vertical supporting seat, a first driving lifting mechanism, a second driving lifting mechanism, a cover opening rotating piece, a lifting fixing plate and an inductor; a cover opening butt joint hole;

The vertical supporting seat is provided with a first driving lifting mechanism and a second driving lifting mechanism, the first driving lifting mechanism can drive the cover opening rotating piece to lift, the second driving lifting mechanism can drive the lifting fixing plate to lift, the inductor is connected with the lifting fixing plate, and the cover opening butt joint hole is formed in the lifting fixing plate.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the cover opening rotating piece comprises a side fixing plate, a middle fixing plate, a driving wheel, a middle driven wheel, a conveyor belt, a conveying gear, a rotating shaft, a spring, connecting teeth and a cover opening rotating head;

The both sides of middle part fixed plate are connected with the side fixed plate, and the top of middle part fixed plate is provided with the roof, and the action wheel passes through the conveyer belt with middle part from the driving wheel to be connected, and the conveyer belt inboard is connected with the transfer gear meshing, and transfer gear is connected with the pivot, and the spring setting is in the outside of pivot, and the pivot lower extreme is connected with the swivel of uncapping through the connection tooth.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a guide rail supporting frame and a sliding clamping piece; the upper end inside the upper operation station is provided with a guide rail supporting frame, a slide rail is arranged on the guide rail supporting frame, and the pipetting component and the sliding clamping piece can move along the slide rail.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the pipetting assembly comprises a pipetting transverse sliding guide rail piece, a pipetting vertical guide rail piece, a second sliding block, a pipetting driving mechanism and a pipetting gun butt joint;

the liquid-transferring transverse sliding guide rail piece is in sliding connection with the sliding rail, the liquid-transferring vertical guide rail piece is in sliding connection with the liquid-transferring transverse sliding guide rail piece, the second sliding block is in vertical sliding connection with the liquid-transferring vertical guide rail piece, and the second sliding block is connected with the liquid-transferring driving mechanism.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the sliding clamping piece comprises a clamping transverse sliding guide rail piece, a clamping first vertical guide rail, a first driving piece, a first sliding block, a clamping second vertical guide rail, a second driving piece, a first clamping part, a sliding rail and a first connecting plate;

The clamping transverse sliding guide rail piece is in sliding connection with the sliding rail, the clamping first vertical guide rail is in sliding connection with the clamping transverse sliding guide rail piece, a first driving piece is arranged on the clamping first vertical guide rail and can drive the first sliding block to vertically slide along the clamping first vertical guide rail, the clamping second vertical guide rail is connected with the first sliding block, the first connecting plate is in vertical sliding connection with the second vertical guide rail, a second driving piece is arranged on the first connecting plate, and the second driving piece is connected with the first clamping part.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a centrifugal tube cell collection assembly, and the centrifugal tube cell collection assembly can collect cells after the centrifugal tube is centrifuged.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the centrifuge tube cell collection assembly comprises a centrifuge funnel fixing seat, a funnel driving piece, a funnel supporting guide rail, a funnel sliding block, a funnel and a fixed storage table;

The upper end of the centrifugal funnel fixing seat is connected with a funnel driving piece, the upper end of the funnel driving piece is provided with a funnel supporting guide rail, the funnel driving piece can drive a funnel sliding block to slide along the funnel supporting guide rail, the front end of the funnel sliding block is provided with a funnel, a fixed storage table is arranged below the funnel, and a centrifuge tube is arranged in the fixed storage table.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the centrifugal component comprises a centrifugal machine and a centrifugal machine supporting frame; the centrifuge support frame supports the centrifuge;

The centrifugal machine comprises a centrifugal shell, a centrifugal driving piece, a centrifugal turntable, a centrifugal rotating shaft, a centrifugal groove, a first photoelectric sensor and a second photoelectric sensor;

Be provided with centrifugal carousel in the centrifugal shell, centrifugal pivot, centrifugal groove, first photoelectric sensor to and second photoelectric sensor, centrifugal driving piece can drive centrifugal carousel and centrifugal pivot rotation, and centrifugal pivot's top sets up a set of centrifugal groove at least, can place the centrifuging tube in the centrifugal groove, and centrifugal shell's upper end has the opening, and opening and last operation station intercommunication.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the side of material loading subassembly is provided with the loading door.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the feeding assembly comprises a feeding moving base, a feeding driving piece, a moving track, a feeding moving plate, a feeding rotary disc driving piece, a feeding rotary disc and a bottle hole site;

a feeding driving piece is arranged on the feeding moving base, a moving track is arranged at the upper end of the feeding driving piece, the feeding driving piece can drive the feeding moving plate to slide along the moving track, the upper end of the feeding moving plate is connected with a feeding turntable driving piece, the feeding turntable driving piece can drive the feeding turntable to rotate, at least one group of storage bottle hole sites are arranged on the feeding turntable, and feeding culture tubes are placed in the storage bottle hole sites.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper end of the upper operation station is provided with a laminar flow hood.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a pipetting head assembly and a balancing weight assembly;

the side of the pipetting head component is provided with a balancing weight component.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the pipetting head assembly comprises a pipetting support frame and a pipetting head;

at least one group of pipetting heads are arranged on the pipetting support frame.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the balancing weight assembly comprises a balancing weight supporting frame and a balancing weight arranged on the balancing weight supporting frame, and at least one group of balancing weights are arranged on the balancing weight supporting frame.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the upper operation station also comprises a waste liquid and waste material collecting mechanism which collects waste water or waste materials.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the waste liquid and waste material collecting mechanism comprises a first waste liquid collecting port, a second waste liquid collecting port, a waste material opposite port, a waste liquid storage tank and a waste material storage tank;

The first waste liquid collection port, the second waste liquid collection port and the waste material opposite connection port are all arranged in the upper operation station, the waste liquid storage tank and the waste material storage tank are arranged below the upper operation station, the first waste liquid collection port and the second waste liquid collection port are discharged into the waste liquid storage tank through pipelines, and the waste material opposite connection port is discharged into the waste material storage tank through pipelines.

As a preferred embodiment of the intelligent cell pipetting workstation of the utility model, wherein: the two side walls of the upper operation station are respectively provided with a front door overturning mechanism and a rear sealing door mechanism.

The utility model has the beneficial effects that: 1. the whole machine has a more intelligent cell preparation process, replaces manual operation, and greatly improves accuracy and working efficiency; 2. the pipetting workstation is more intelligent in feeding and discharging, realizes rapid cell preparation by matching the built-in manipulator with the sliding clamping piece, and meets the standard requirements better; 3. the batch type whole-plate uncapping mechanism can realize the uncapping action of the rapid whole-plate type sample tube by matching the sample box supporting plate with the manipulator, and greatly improves the efficiency of manually opening the upper cover of the sample tube;

4. The feeding assembly is connected with the feeding culture tube in an opposite way through automation and the outside, intelligently connected with an external window in an opposite way, and improves the working efficiency; 5. the manipulator of the pipetting workstation is flexible and reliable, and is matched with a plurality of quick-change heads, so that the clamping part of the manipulator can be quickly replaced, different grabbing requirements can be adapted, the grabbing accuracy can be improved, and the stability of shaking culture bottles can be enhanced; 6. the pipetting assembly can be used for rapidly pipetting and discharging waste liquid.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is an overall schematic of an intelligent cell pipetting workstation.

FIG. 2 is a rear seal gate mechanism of an intelligent cell pipetting workstation.

FIG. 3 is a schematic diagram of the interior of the upper station of the intelligent cell pipetting workstation.

FIG. 4 is a schematic view of a slide gripper of an intelligent cell pipetting station.

FIG. 5 is a schematic of a pipetting assembly of the intelligent cell pipetting workstation.

FIG. 6 is a schematic diagram of the interior of the upper station of the intelligent cell pipetting workstation.

FIG. 7 is a schematic view of a rail support frame of an intelligent cell pipetting workstation.

FIG. 8 is a schematic view of a sample cartridge storage station assembly of an intelligent cell pipetting workstation.

FIG. 9 is a centrifuge tube storage station assembly of the intelligent cell pipetting workstation.

FIG. 10 is a schematic diagram of a flask storage stage assembly of an intelligent cell pipetting workstation.

FIG. 11 is a schematic view of a gripper head support of an intelligent cell pipetting workstation.

FIG. 12 is a waste liquid and waste collection mechanism of an intelligent cell pipetting workstation.

FIG. 13 is a pipetting head assembly of the intelligent cell pipetting workstation.

FIG. 14 is a schematic diagram of a centrifuge tube cell collection assembly of an intelligent cell pipetting workstation.

FIG. 15 is a schematic diagram of an uncapping assembly of an intelligent cell pipetting workstation.

FIG. 16 is a schematic view of the interior of the uncapping assembly of the intelligent cell pipetting station.

FIG. 17 is a rear view of an open cover assembly of the intelligent cell pipetting workstation.

FIG. 18 is a schematic diagram of an open cover rotor of an intelligent cell pipetting workstation.

FIG. 19 is a schematic view of a lifting fixture plate of an intelligent cell pipetting workstation.

FIG. 20 is a schematic diagram of a loading assembly of an intelligent cell pipetting workstation.

FIG. 21 is a schematic diagram of a centrifuge assembly of an intelligent cell pipetting workstation.

FIG. 22 is a schematic diagram of the interior of a centrifuge assembly of an intelligent cell pipetting workstation.

Reference numerals:

An upper operation station 1; a centrifugal assembly 6; a feeding assembly 2; a feeding culture tube; 208; a pipetting assembly, 101; a grasping assembly, 102; centrifuge tubes, 10405; a sample cartridge storage stage assembly 106; centrifuge tube storage rack assembly, 104; a culture solution storage table assembly 103; a flask storage stand assembly 107; a flask, 10705; a sample cartridge drive, 10601; a sample cartridge support plate, 10602; sample cartridge support plate shaft, 10603; a sample cartridge, 10605; centrifuge tube carousel drive, 10401; centrifuge tube carousel, 10402; an upper storage rack 10403; A lower fixed seat 10404; centrifuge tube fixtures, 10406; a culture fluid supporting plate 10301; a culture solution supporting plate driving member 10302; storing bottle hole sites, 10303; a flask driver, 10701; a flask support plate, 10703; a flask storage tank 10704; a robot arm 1021; a slide clamp 1022; a robot arm 102101; a gripper head support 102102; the first hand grip quick change head 102103; a second gripper quick change head 102104; a third hand grip quick change head 102105; an uncap assembly, 108; vertical supporting seat 10801; a first drive lift mechanism 10802; a second drive lifting mechanism 10803; cover opening rotor 10804; lifting a fixing plate 10805; a sensor 10806; uncapping the butt joint hole, 10807; a side fixing plate 1080401; a middle fixing plate 1080402; a driving wheel 1080403; a middle driven wheel 1080404; a conveyor belt 1080405; a transfer gear 1080406; a rotating shaft 1080407; a spring 1080408; a connecting tooth 1080409; cover opening swivel 1080410; A rail support 111; a slide clamp 1022; a slide rail 112; a pipetting lateral sliding rail member 10101; pipetting vertical guide rail piece 10102; a second slider 10103; a pipetting drive mechanism 10104; a pipette butt joint 10105; clamping the transverse sliding rail member 102201; clamping a first vertical rail 102202; a first driving member 102203; a first slider 102204; clamping a second vertical guide rail 102205; a second driving member 102207; a first clamp 102208; a slide rail 112; A first connection plate 102209; a centrifugal funnel holder 11301; a funnel drive 11302; funnel support rails 11303; funnel slider, 11304; a funnel 11305; a fixed storage table 11306; a centrifuge 61; a centrifuge support frame 62; centrifuge housing 6102; centrifugal drive 6103; centrifugal turntable 6104; centrifugal shaft 6105; centrifugal tank 6106; a first photosensor 6107; a second photosensor 6108; a feeding door 3; a feeding mobile base 201; a feeding driving piece 202; a moving track 203; a feeding moving plate 204; a loading turntable driving part 205; a loading turntable 206; storing bottle hole sites, 10303; a laminar flow hood 4; a pipetting head assembly 105; a counterweight assembly 114; pipetting support frame 10501; pipetting head, 10502; a counterweight support frame 11401; balancing weight 11402; a waste liquid and waste collection mechanism 115; a first waste collection port, 11501; a second waste collection port, 11502; waste-to-interface 11503; a waste liquid storage tank 11504; a waste holding tank 11505; a front roll-over door mechanism, 116; A rear seal door mechanism 117;

Detailed Description

In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model, but the present utility model may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present utility model is not limited to the specific embodiments disclosed below.

Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the utility model. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Example 1

Referring to fig. 1 to 6, in a first embodiment of the present utility model, an intelligent cell pipetting workstation is provided, which includes an upper operation station 1, a centrifugal component 6 and a feeding component 2, wherein the upper operation station 1 is disposed above the centrifugal component 6 and the feeding component 2, the upper operation station 1 includes a pipetting component 101, a grabbing component 102 and an operation table, a cell liquid can be sucked into a centrifugal tube 10405 through the pipetting component 101, a feeding culture tube 208 on the feeding component 2 can be clamped by the grabbing component 102, and is placed on the operation table after entering the upper operation station 1, a centrifugal tube 10405 can be grabbed into the centrifugal component 6 through the grabbing component 102, and cells can be separated from the centrifugal tube 10405 through the centrifugal component 6.

Specifically, comprises an upper operation station 1, a centrifugal assembly 6 and a feeding assembly 2,

The upper operating station 1 is arranged above the centrifugal component 6 and the feeding component 2,

The feeding component 2 can receive the feeding culture tube 208, and the centrifugal component 6 can centrifuge the cell sap;

The upper station 1 comprises a pipetting module 101, a gripping module 102 and a console,

A centrifuge tube 10405 can be placed on the operation table, the pipetting component 101 can suck cell liquid into the centrifuge tube 10405, the grabbing component 102 can clamp the feeding culture tube 208 on the feeding component 2 to enter the upper operation station 1 to be placed on the operation table,

The pipetting assembly 101 can suck the culture solution in the feeding culture tube 208 into a centrifuge tube 10405;

The gripper assembly 102 may grip the centrifuge tube 10405 into the centrifuge assembly 6.

In summary, according to the utility model, the pipetting assembly 101 is used for sucking the cell liquid and the culture liquid, the pipetting assembly 101 is used for placing the cell liquid and the culture liquid into the centrifuge tube 10405, the grabbing assembly 102 is used for clamping the feeding culture tube 208 on the feeding assembly 2, the feeding culture tube 208 enters the upper operation station 1 and is placed on the operation table, the grabbing assembly 102 is used for grabbing the centrifuge tube 10405, and the centrifuge assembly 6 is used for centrifuging the cells, so that intelligent cell preparation is realized, manual work is replaced, and accuracy and working efficiency are greatly improved.

Example 2

Referring to fig. 1 to 22, in the above embodiment, the intelligent cell pipetting workstation comprises an upper operation station 1, a centrifugal component 6 and a feeding component 2, wherein the upper operation station 1 is arranged above the centrifugal component 6 and the feeding component 2, the upper operation station 1 comprises a pipetting component 101, a grabbing component 102 and an operation table, cell liquid can be sucked into a centrifugal tube 10405 through the pipetting component 101, a feeding culture tube 208 on the feeding component 2 can be clamped by the grabbing component 102, the feeding culture tube 208 enters the upper operation station 1 and is placed on the operation table, the centrifugal tube 10405 can be grabbed into the centrifugal component 6 through the grabbing component 102, and cells can be separated from the centrifugal tube 10405 through the centrifugal component 6.

Specifically, comprises an upper operation station 1, a centrifugal assembly 6 and a feeding assembly 2,

The upper operating station 1 is arranged above the centrifugal component 6 and the feeding component 2,

The feeding component 2 can receive the feeding culture tube 208, and the centrifugal component 6 can centrifuge the cell sap;

The upper station 1 comprises a pipetting module 101, a gripping module 102 and a console,

A centrifuge tube 10405 can be placed on the operation table, the pipetting component 101 can suck cell liquid into the centrifuge tube 10405, the grabbing component 102 can clamp the feeding culture tube 208 on the feeding component 2 to enter the upper operation station 1 to be placed on the operation table,

The pipetting assembly 101 can suck the culture solution in the feeding culture tube 208 into a centrifuge tube 10405;

The gripper assembly 102 may grip the centrifuge tube 10405 into the centrifuge assembly 6.

Further, the upper operation station 1 further comprises a sample box storage table assembly 106, a centrifuge tube storage table assembly 104, a culture solution storage table assembly 103, and a culture bottle storage table assembly 107;

At least one group of centrifuge tubes 10405 are stored on the centrifuge tube storage table assembly 104, at least one group of freezing tubes are stored on the sample box storage table assembly 106, at least one group of feeding culture tubes 208 are stored on the culture solution storage table assembly 103, and at least one group of culture bottles 10705 are stored on the culture bottle storage table assembly 107;

the sample box storage table assembly 106, the centrifuge tube storage table assembly 104, the culture solution storage table assembly 103 and the culture bottle storage table assembly 107 can be arranged in a rotation or non-rotation fixed mode around the axis of the culture bottle storage table assembly;

Preferably, the centrifuge tube storage table assembly 104, the culture solution storage table assembly 103 and the culture flask storage table assembly 107 can be more convenient to grasp and place around the rotation of the axis, and the sample box storage table assembly 106 can transfer one whole freezing tube to the lower part of the cover opening assembly 108 by rotating, so that the freezing tube bottle cap can be conveniently opened for the whole freezing tube.

The pipetting assembly 101 can aspirate the cell liquid in the cryopreservation vessel into the centrifuge tube 10405 and the culture flask 10705, and the pipetting assembly 101 can aspirate the complete culture medium in the feeding culture vessel 208 into the culture flask 10705.

Preferably, the pipetting drive mechanism 10104 drives the pipetting head 10502 through the pipetting gun to the interface 10105 to draw 1ml of seed cells containing frozen solution from the frozen storage tube in the sample box storage table assembly 106, then place the seed cells into the centrifuge tube 10405, replace the pipetting head 10502 after each placement of the solution, then the pipetting drive mechanism 10104 drives the pipetting head 10502 through the pipetting gun to draw 9ml of complete culture medium from the feeding culture tube 208 on the culture liquid storage table assembly 103 to place the complete culture medium into the centrifuge tube 10405, then the second drive member 102207 drives the first clamping part 102208 to screw up the tube cover of the centrifuge tube 10405, and then place the centrifuge tube 10405 into the centrifuge 61 for centrifugation; after centrifugation is completed, the centrifuge tube 10405 in the centrifuge 61 is placed on the centrifuge tube turntable 10402 by the first clamping portion 102208, the bottom of the centrifuge tube 10405 is fixed by the centrifuge tube fixing member 10406, then the tube cover of the centrifuge tube 10405 is opened by driving the first clamping portion 102208 by the second driving member 102207, then the waste liquid is sucked from the centrifuge tube 10405 by the pipetting driving mechanism 10104 through the pipetting gun interface 10105 by driving the pipetting head 10502, collected into the waste liquid storage tank 11504 through the first waste liquid collecting opening 11501, and then 15ml of complete culture medium is sucked from the feeding culture tube 208 on the culture liquid storage table assembly 103 by the pipetting driving mechanism 10104 through the pipetting gun interface 10105 and placed into the centrifuge tube 10405 for suspension sedimentation.

Preferably, the pipetting drive mechanism 10104 drives the pipetting head 10502 through the pipetting gun to the interface 10105 to draw complete medium from the feed tube 208 on the culture medium storage table assembly 103 and place it in three culture bottles 10705, 30ml of complete medium is placed in each culture bottle 10705, then the pipetting drive mechanism 10104 drives the pipetting head 10502 through the pipetting gun to draw cell liquid from the cryopreservation tube in the sample box storage table assembly 106 and place it in three culture bottles 10705, 5ml is placed in each culture bottle 10705, and then the second drive member 102207 drives the first clamping portion 102208 to screw the caps of the culture bottles 10705 to wait for the next operation.

Preferably, the sample box storage table assembly 106 can store a plurality of groups of whole-plate freezing storage tubes, and cell liquid is placed in the freezing storage tubes; a plurality of groups of feeding culture tubes 208 are stored in the culture solution storage table assembly 103, and 4 kinds of liquid including complete culture medium, buffer solution, passage solution and frozen stock solution can be placed in the plurality of groups of feeding culture tubes 208;

Preferably, the culture fluid storage station assembly 103 and centrifuge tube storage station assembly 104 form the station described above.

Further, the cartridge storage table assembly 106 includes a cartridge drive 10601, a cartridge support plate 10602, a cartridge support plate shaft 10603, and a cartridge 10605;

the sample box driving piece 10601 drives the sample box supporting plate 10602 to rotate through the sample box supporting plate rotating shaft 10603, at least one group of sample box position grooves 10604 are formed in the sample box supporting plate 10602, sample boxes 10605 can be stored in the sample box position grooves 10604, and freezing pipes can be stored in the sample boxes 10605.

Preferably, the cartridge driving member 10601 drives the cartridge supporting plate shaft 10603 to rotate, and the cartridge supporting plate shaft 10603 drives the cartridge supporting plate 10602 to rotate, i.e. the cartridge 10605 rotates.

Preferably, the cartridge drive 10601, centrifuge tube carousel drive 10401, culture support plate drive 10302, and culture bottle drive 10701 may be motor driven, cylinder driven, or other prior art drive means.

Further, the centrifuge tube storage table assembly 104 comprises a centrifuge tube turntable drive 10401, a centrifuge tube turntable 10402, an upper storage rack 10403, a lower fixed seat 10404, and a centrifuge tube fixing 10406;

Centrifuge tube carousel drive piece 10401 is connected with centrifuge tube carousel 10402, is provided with at least a set of upper storage rack 10403 and lower fixing base 10404 on the centrifuge tube carousel 10402, and centrifuge tube 10405 can be placed in upper storage rack 10403 and lower fixing base 10404, and centrifuge tube fixing piece 10406 can be fixed the lower extreme of centrifuge tube 10405 placed in lower fixing base 10404.

Preferably, a weight 11402 may be placed in centrifuge tube turntable 10402 to maintain stability.

Preferably, can fix the bottom of single tube centrifuging tube 10405 through centrifuging tube mounting 10406, make things convenient for manipulator 102101 to open the tube cap of centrifuging tube 10405.

Preferably, centrifuge tube mount 10406 includes driving motor and centrifuge tube clamping jaw, and driving motor can drive the centrifuge tube clamping jaw and fix centrifuging tube 10405.

Further, the culture medium storing table assembly 103 comprises a culture medium supporting plate 10301, a culture medium supporting plate driving member 10302 and a storing bottle hole site 10303;

The culture medium supporting plate driving piece 10302 drives the culture medium supporting plate 10301 to rotate, at least one group of storage bottle hole sites 10303 are arranged on the culture medium supporting plate 10301, and the feeding culture tubes 208 can be placed in the storage bottle hole sites 10303.

Preferably, the manipulator 102101 is configured to complete the feeding process by grasping the feeding tube 208 of the feeding assembly 2 and placing it into the storage bottle hole 10303 on the culture fluid support plate 10301.

Preferably, the manipulator 102101 is composed of multiple rotational shafts, so that multi-angle and multi-directional rotation can be realized.

Further, the flask storage stand assembly 107 includes a flask drive 10701, a flask support plate 10703, and a flask storage slot 10704;

The culture bottle driving piece 10701 is connected with the culture bottle supporting plate 10703, at least one group of culture bottle storage grooves 10704 are formed in the culture bottle supporting plate 10703, and culture bottles 10705 can be stored in the culture bottle storage grooves 10704; the flask driving member 10701 may drive rotation of the flask supporting plate 10703.

Further, the gripper assembly 102 includes a robot 1021 and a slide gripper 1022;

the robot 1021 can grasp the feeding culture tube 208 on the feeding assembly 2, enter the upper operation station 1 and be placed on the culture solution storage table assembly 103, and the sliding clamping member 1022 can grasp the centrifuge tube 10405 and enter the centrifuge assembly 6 for centrifugation.

Further, the robot 1021 includes a robot 102101;

a gripper head support 102102 provided on one side of the robot 102101;

The first hand grip quick change head 102103, the second hand grip quick change head 102104, and the third hand grip quick change head 102105 are provided on the hand grip support 102102.

Preferably, the first hand-grip quick-change head 102103, the second hand-grip quick-change head 102104 and the third hand-grip quick-change head 102105 are different types of quick-change heads, so that the manipulator 102101 can be conveniently and quickly replaced, and the feeding culture tube 208, the centrifuge tube 10405 and the culture bottle 10705 can be conveniently grabbed.

Further, the upper operation station 1 further comprises an opening assembly 108, and the opening assembly 108 can open the freezing tube in the sample box storage table assembly 106 integrally.

Further, the lid opening assembly 108 includes a vertical support 10801, a first driving lifter 10802, a second driving lifter 10803, a lid opening rotator 10804, a lifting fixing plate 10805, and a sensor 10806; uncapping docking aperture 10807;

the vertical support 10801 is provided with a first driving lifting mechanism 10802 and a second driving lifting mechanism 10803, the first driving lifting mechanism 10802 can drive the cover opening rotating member 10804 to lift, the second driving lifting mechanism 10803 can drive the lifting fixing plate 10805 to lift, the sensor 10806 is connected with the lifting fixing plate 10805, and the lifting fixing plate 10805 is provided with a cover opening butt joint hole 10807.

Preferably, the first driving lifting mechanism 10802 and the second driving lifting mechanism 10803 may adopt a device that a motor drives a screw rod, the two screw rods may respectively drive the cover opening rotating member 10804 and the lifting fixing plate 10805 in threaded connection with the two screw rods to lift under the driving of the motor, and other lifting mechanisms in the prior art may be adopted, and the sensor 10806 may sense the position of the nut of the freezing tube, so as to control the second driving lifting mechanism 10803 to stop lifting.

Preferably, the second driving lifting mechanism 10803 can drive the lifting fixing plate 10805 to press the whole freezing tube.

Further, the cover opening rotator 10804 includes a side fixing plate 1080401, a middle fixing plate 1080402, a driving wheel 1080403, a middle driven wheel 1080404, a conveyor 1080405, a transfer gear 1080406, a rotation shaft 1080407, a spring 1080408, connection teeth 1080409, and a cover opening rotator 1080410;

The both sides of middle part fixed plate 1080402 are connected with side fixed plate 1080401, and the top of middle part fixed plate 1080402 is provided with the roof, and action wheel 1080403 passes through conveyer belt 1080405 with middle part from driving wheel 1080404 to be connected, and conveyer belt 1080405 inboard is connected with the meshing of transfer gear 1080406, and transfer gear 1080406 is connected with pivot 1080407, and spring 1080408 sets up in the outside of pivot 1080407, and pivot 1080407 lower extreme is connected with lid-opening swivel 1080410 through connecting tooth 1080409.

Preferably, the cover opening swivel 1080410 can be inserted into the cap of the cryopreservation vessel.

Preferably, the driving motor can drive the driving wheel 1080403 to rotate, so that the middle driven wheel 1080404 is driven to rotate by the conveying belt 1080405, meanwhile, the conveying belt 1080405 can drive the conveying gear 1080406 to rotate, the conveying gear 1080406 drives the rotating shaft 1080407 and the connecting teeth 1080409 to rotate, the connecting teeth 1080409 drive the cover opening rotating head 1080410 to rotate, and the cover opening rotating head 1080410 can drive the pipe cap of the freezing pipe to rotate, so that the pipe cap is opened.

Further, the upper operation station 1 further includes a rail supporting frame 111 and a sliding gripping member 1022; the upper end of the interior of the upper operation station 1 is provided with a guide rail supporting frame 111, the guide rail supporting frame 111 is provided with a slide rail 112, and the pipetting assembly 101 and the slide gripping member 1022 can move along the slide rail 112.

Further, the pipetting assembly 101 comprises a pipetting transverse sliding rail member 10101, a pipetting vertical rail member 10102, a second slider 10103, a pipetting drive mechanism 10104, and a pipetting gun interface 10105;

the pipetting horizontal sliding rail piece 10101 is in sliding connection with the sliding rail 112, the pipetting vertical rail piece 10102 is in sliding connection with the pipetting horizontal sliding rail piece 10101, the second slider 10103 is in vertical sliding connection with the pipetting vertical rail piece 10102, and the second slider 10103 is connected with the pipetting driving mechanism 10104.

Preferably, the pipetting drive mechanism 10104 and the pipetting head 10502 can effect movement of the XYZ axes.

Preferably, the motor can drive the horizontal sliding guide rail member 10101 to be in sliding connection with the sliding rail 112, the pipetting vertical guide rail member 10102 can be in sliding connection with the pipetting horizontal sliding guide rail member 10101, the second sliding block 10103 can be driven by the motor or other driving mechanisms to be in vertical sliding connection along the pipetting vertical guide rail member 10102, different TIP heads can be replaced through the pipetting gun butt joint opening 10105, the pipetting gun butt joint opening 10105 can be butt joint with the pipetting head 10502, and the pipetting head 10502 needs to be replaced after each time of liquid suction, so that the liquid is not polluted, and the situation that various liquids are mixed is avoided.

Further, the upper operation station 1 further comprises a pipetting head assembly 105 and a balancing weight assembly 114;

a weight block assembly 114 is provided laterally of the pipetting head assembly 105.

Further, the pipetting head assembly 105 includes a pipetting support 10501, and a pipetting head 10502;

At least one set of pipetting heads 10502 is provided on the pipetting support 10501.

Further, the counterweight assembly 114 includes a counterweight support 11401, and a counterweight 11402 disposed on the counterweight support 11401, wherein at least one set of counterweights 11402 is disposed on the counterweight support 11401.

Further, the sliding gripping member 1022 includes a gripping lateral sliding rail member 102201, a gripping first vertical rail 102202, a first driving member 102203, a first slider 102204, a gripping second vertical rail 102205, a second driving member 102207, a first gripping portion 102208, a sliding rail 112, and a first connecting plate 102209;

The clamping transverse sliding guide rail member 102201 is slidably connected with the sliding rail 112, the clamping first vertical guide rail 102202 is slidably connected with the clamping transverse sliding guide rail member 102201, a first driving member 102203 is arranged on the clamping first vertical guide rail 102202, the first driving member 102203 can drive the first sliding block 102204 to vertically slide along the clamping first vertical guide rail 102202, the clamping second vertical guide rail 102205 is connected with the first sliding block 102204, the first connecting plate 102209 is vertically slidably connected with the second vertical guide rail 102205, a second driving member 102207 is arranged on the first connecting plate 102209, and the second driving member 102207 is connected with the first clamping portion 102208.

Preferably, the second driving member can drive the first clamping portion 102208 to clamp the centrifuge tube 10405, and the second driving member can also drive the first clamping portion 102208 to rotate, so as to open the cap of the centrifuge tube 10405.

Preferably, the first driving member 102203 and the first driving member 102203 can be driven by a motor, or can be driven by other driving means, the driving motor or other driving mechanism can drive the first connecting plate 102209 to slide vertically along the second vertical rail 102205, and the second driving member 102207 can drive the first clamping portion 102208 to grab the centrifuge tube 10405.

Preferably, the second driving member 102207 and the first clamping portion 102208 can realize movement of XYZ axes.

Further, the upper operation station 1 further comprises a centrifugal tube cell collecting assembly 113, and the centrifugal tube cell collecting assembly 113 can collect cells after centrifugation of the centrifugal tube 10405.

Further, centrifuge tube cell collection assembly 113 comprises centrifuge funnel holder 11301, funnel drive 11302, funnel support rail 11303, funnel slider 11304, funnel 11305, and stationary storage table 11306;

The upper end of the centrifugal funnel fixing base 11301 is connected with a funnel driving piece 11302, a funnel supporting guide rail 11303 is arranged at the upper end of the funnel driving piece 11302, the funnel driving piece 11302 can drive a funnel sliding block 11304 to slide along the funnel supporting guide rail 11303, a funnel 11305 is arranged at the front end of the funnel sliding block 11304, a fixed storage table 11306 is arranged below the funnel 11305, and a centrifugal tube 10405 is arranged in the fixed storage table 11306.

Preferably, funnel 11305 can be advanced or retracted with funnel slider 11304, and multiple tubes of cells can be combined into one tube by funnel 11305.

Further, the centrifuge assembly 6 includes a centrifuge 61, and a centrifuge support frame 62; the centrifuge support frame 62 supports the centrifuge 61;

The centrifuge 61 includes a centrifuge housing 6102, a centrifuge driver 6103, a centrifuge rotor 6104, a centrifuge shaft 6105, a centrifuge tank 6106, a first photosensor 6107, and a second photosensor 6108;

The centrifugal shell 6102 is internally provided with a centrifugal rotary table 6104, a centrifugal rotary shaft 6105, a centrifugal tank 6106, a first photoelectric sensor 6107 and a second photoelectric sensor 6108, the centrifugal driving member 6103 can drive the centrifugal rotary table 6104 and the centrifugal rotary shaft 6105 to rotate, at least one group of centrifugal tanks 6106 are arranged above the centrifugal rotary shaft 6105, centrifugal tubes 10405 can be placed in the centrifugal tanks 6106, an opening is arranged at the upper end of the centrifugal shell 6102, and the opening is communicated with the upper operation station 1.

Preferably, centrifuge 61 can centrifuge tube 10405.

Preferably, a weight 11402 can be further disposed in the centrifugal groove 6106.

Further, the side of the feeding component 2 is provided with a feeding door 3.

Preferably, the loading door 3 can be opened, and the feeding assembly 2 can be supplemented by the loading door 3.

Further, the feeding assembly 2 comprises a feeding moving base 201, a feeding driving piece 202, a moving track 203, a feeding moving plate 204, a feeding rotary table driving piece 205, a feeding rotary table 206 and a bottle storing hole site 10303;

The feeding mobile base 201 is provided with a feeding driving piece 202, the upper end of the feeding driving piece 202 is provided with a mobile track 203, the feeding driving piece 202 can drive a feeding mobile plate 204 to slide along the mobile track 203, the upper end of the feeding mobile plate 204 is connected with a feeding rotary table driving piece 205, the feeding rotary table driving piece 205 can drive a feeding rotary table 206 to rotate, at least one group of storage bottle hole sites 10303 are arranged on the feeding rotary table 206, and feeding culture tubes 208 are placed in the storage bottle hole sites 10303.

Preferably, the feeding turntable 206 can supplement feeding for the purpose of rotation, and the manipulator 102101 can conveniently grip during rotation.

Further, the upper end of the upper operation station 1 is provided with a laminar flow hood 4.

Preferably, the air passes through the high-efficiency filter at a certain air speed through the laminar flow hood 4 to form a uniform flow layer, so that clean air is vertically and unidirectionally flowed, and the cleanliness reaching the process requirement in a working area is ensured.

Further, the upper operation station 1 further includes a waste liquid and waste material collecting mechanism 115, and the waste liquid and waste material collecting mechanism 115 collects waste water or waste material.

Further, the waste liquid and waste collection mechanism 115 includes a first waste liquid collection port 11501, a second waste liquid collection port 11502, a waste docking port 11503, a waste liquid storage tank 11504, and a waste storage tank 11505;

The first waste liquid collection port 11501, the second waste liquid collection port 11502 and the waste docking port 11503 are both disposed in the upper operation station 1, the waste liquid storage tank 11504 and the waste storage tank 11505 are disposed below the upper operation station 1, the first waste liquid collection port 11501 and the second waste liquid collection port 11502 are discharged into the waste liquid storage tank 11504 through a pipe, and the waste docking port 11503 is discharged into the waste storage tank 11505 through a pipe.

Further, the front door tilting mechanism 116 and the rear sealing door mechanism 117 are respectively provided on both side walls of the upper operation station 1.

Preferably, the front side door is openable by a front flap door mechanism 116 and the rear side door is openable by a rear seal door mechanism 117.

In summary, according to the utility model, the pipetting assembly 101 is used for sucking the cell liquid and the culture liquid in the freezing tube in the sample box storage table assembly 106, placing the cell liquid and the culture liquid in the centrifuge tube 10405, clamping the feeding culture tube 208 on the feeding assembly 2 by the manipulator 102101, entering the upper operation station 1, and grabbing the centrifuge tube 10405 by the sliding clamping member 1022, entering the centrifuge assembly 6 for centrifuging cells; the pipetting assembly 101 is used for sucking the cell liquid in the freezing tube in the sample box storage table assembly 106, placing the cell liquid in the culture bottle 10705, and the pipetting assembly 101 is used for sucking the culture medium of the feeding culture tube 208 in the culture liquid storage table assembly 103, and then the cell liquid is fed into the culture bottle 10705 to wait for the next cell culture; the device greatly improves the working efficiency.

It is important to note that the construction and arrangement of the utility model as shown in the various exemplary embodiments is illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters (e.g., temperature, pressure, etc.), mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter described in this application. For example, elements shown as integrally formed may be constructed of multiple parts or elements, the position of elements may be reversed or otherwise varied, and the nature or number of discrete elements or positions may be altered or varied. Accordingly, all such modifications are intended to be included within the scope of present utility model. The order or sequence of any process or method steps may be varied or re-sequenced according to alternative embodiments. In the claims, any means-plus-function clause is intended to cover the structures described herein as performing the recited function and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present utility models. Therefore, the utility model is not limited to the specific embodiments, but extends to various modifications that nevertheless fall within the scope of the appended claims.

Furthermore, in order to provide a concise description of the exemplary embodiments, all features of an actual implementation may not be described (i.e., those not associated with the best mode presently contemplated for carrying out the utility model, or those not associated with practicing the utility model).

It should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort might be complex and time consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure.

It should be noted that the above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present utility model may be modified or substituted without departing from the spirit and scope of the technical solution of the present utility model, which is intended to be covered in the scope of the claims of the present utility model.

Claims (26)

1. An intelligent cell pipetting workstation which is characterized in that: comprises an upper operation station (1), a centrifugal component (6) and a feeding component (2),

The upper operation station (1) is arranged above the centrifugal component (6) and the feeding component (2),

The feeding component (2) can receive a feeding culture tube (208), and the centrifugal component (6) can centrifuge cell sap;

The upper operation station (1) comprises a pipetting component (101), a grabbing component (102) and an operation table,

The centrifuge tube (10405) can be placed on the operating platform, the pipetting component (101) can suck cell sap into the centrifuge tube (10405), the grabbing component (102) can clamp the feeding culture tube (208) on the feeding component (2) to enter the upper operating station (1) to be placed on the operating platform,

The pipetting component (101) can suck the culture solution in the feeding culture tube (208) into a centrifuge tube (10405);

The grabbing component (102) can grab the centrifuge tube (10405) to enter the centrifugal component (6).

2. The intelligent cell pipetting workstation of claim 1 wherein: the upper operation station (1) further comprises a sample box storage table assembly (106), a centrifuge tube storage table assembly (104), a culture solution storage table assembly (103) and a culture bottle storage table assembly (107);

At least one group of centrifuge tubes (10405) are stored on the centrifuge tube storage table assembly (104), at least one group of freezing storage tubes are stored on the sample box storage table assembly (106), at least one group of feeding culture tubes (208) are stored on the culture solution storage table assembly (103), and at least one group of culture bottles (10705) are stored on the culture bottle storage table assembly (107);

The sample box storage table assembly (106), the centrifuge tube storage table assembly (104), the culture solution storage table assembly (103) and the culture bottle storage table assembly (107) can be all arranged in a autorotation or non-rotation fixed mode around the axis of the culture bottle storage table assembly;

The pipetting component (101) can suck cell sap in the freezing tube into the centrifuge tube (10405) and the culture bottle (10705), and the pipetting component (101) can suck complete culture medium in the feeding culture tube (208) into the culture bottle (10705).

3. The intelligent cell pipetting workstation of claim 2 wherein: the cartridge storage stage assembly (106) includes a cartridge drive (10601), a cartridge support plate (10602), a cartridge support plate spindle (10603), and a cartridge (10605);

Sample box driving piece (10601) drives through sample box backup pad pivot (10603) sample box backup pad (10602) rotates, set up a set of sample box standing groove (10604) at least on sample box backup pad (10602), can deposit sample box (10605) in sample box standing groove (10604), can deposit the cryopreservation pipe in sample box (10605).

4. The intelligent cell pipetting workstation of claim 2 wherein: the centrifuge tube storage table assembly (104) comprises a centrifuge tube turntable driving piece (10401), a centrifuge tube turntable (10402), an upper storage rack (10403), a lower fixing seat (10404) and a centrifuge tube fixing piece (10406);

centrifuge tube carousel driving piece (10401) with centrifuge tube carousel (10402) is connected, be provided with storage rack (10403) and lower fixing base (10404) on centrifuge tube carousel (10402) on at least a set of, centrifuging tube (10405) can be placed in storage rack (10403) and lower fixing base (10404), centrifuging tube fixing piece (10406) can be fixed the lower extreme of centrifuging tube (10405) of placing in lower fixing base (10404).

5. The intelligent cell pipetting workstation of claim 2 wherein: the culture solution storage table assembly (103) comprises a culture solution supporting plate (10301), a culture solution supporting plate driving piece (10302) and a storage bottle hole site (10303);

The culture solution supporting plate driving piece (10302) drives the culture solution supporting plate (10301) to rotate, at least one group of storage bottle hole sites (10303) are arranged on the culture solution supporting plate (10301), and a feeding culture tube (208) can be placed in the storage bottle hole sites (10303).

6. The intelligent cell pipetting workstation of claim 2 wherein: the culture bottle storage table assembly (107) comprises a culture bottle driving piece (10701), a culture bottle supporting plate (10703) and a culture bottle storage groove (10704);

The culture bottle driving piece (10701) is connected with the culture bottle supporting plate (10703), at least one group of culture bottle storage grooves (10704) are formed in the culture bottle supporting plate (10703), and culture bottles (10705) can be stored in the culture bottle storage grooves (10704); the culture bottle driving piece (10701) can drive the culture bottle supporting plate (10703) to rotate.

7. The intelligent cell pipetting workstation of claim 2 wherein: the grabbing assembly (102) comprises a robot arm (1021) and a sliding clamping piece (1022);

The manipulator piece (1021) can grab a feeding culture tube (208) on the feeding assembly (2) to enter the upper operation station (1) and be placed on the culture solution storage table assembly (103), and the sliding clamping piece (1022) can grab a grabbing centrifuge tube (10405) to enter the centrifuge assembly (6) for centrifugation.

8. The intelligent cell pipetting workstation of claim 7 wherein: the robot arm (1021) includes a robot arm (102101);

A gripper head support frame (102102) arranged on one side of the manipulator (102101);

The gripper head support frame (102102) is provided with a first gripper quick-change head (102103), a second gripper quick-change head (102104) and a third gripper quick-change head (102105).

9. The intelligent cell pipetting workstation of claim 2 wherein: the upper operation station (1) further comprises a cover opening assembly (108), and the cover opening assembly (108) can integrally cover the freezing storage tube in the sample box storage table assembly (106).

10. The intelligent cell pipetting workstation of claim 9 wherein: the cover opening assembly (108) comprises a vertical supporting seat (10801), a first driving lifting mechanism (10802), a second driving lifting mechanism (10803), a cover opening rotating piece (10804), a lifting fixing plate (10805) and a sensor (10806); a door-opening mating hole (10807);

Be provided with first drive elevating system (10802) and second drive elevating system (10803) on vertical support (10801), first drive elevating system (10802) can drive uncap rotor (10804) goes up and down, second drive elevating system (10803) can drive lift fixed plate (10805) goes up and down, inductor (10806) with lift fixed plate (10805) are connected, uncap butt joint hole (10807) have been seted up on lift fixed plate (10805).

11. The intelligent cell pipetting workstation of claim 10 wherein: the cover opening rotating piece (10804) comprises a side fixing plate (1080401), a middle fixing plate (1080402), a driving wheel (1080403), a middle driven wheel (1080404), a conveying belt (1080405), a conveying gear (1080406), a rotating shaft (1080407), a spring (1080408), connecting teeth (1080409) and a cover opening rotating head (1080410);

The utility model discloses a cover opening device, including middle part fixed plate (1080402), top fixed plate (1080402) and connecting gear (1080406), the both sides of middle part fixed plate (1080402) are connected with side fixed plate (1080401), the top of middle part fixed plate (1080402) is provided with the roof, action wheel (1080403) with middle part is from driving wheel (1080404) through conveyer belt (1080405) are connected, conveyer belt (1080405) inboard with conveying gear (1080406) meshing is connected, conveying gear (1080406) with pivot (1080407) are connected, spring (1080408) set up the outside of pivot (1080407), pivot (1080407) lower extreme through connecting tooth (1080409) with cover opening swivel (1080410) are connected.

12. The intelligent cell pipetting workstation of claim 2 wherein: the upper operation station (1) also comprises a guide rail supporting frame (111) and a sliding clamping piece (1022); the upper operation station (1) is provided with a guide rail support frame (111) at the upper end inside, a slide rail (112) is arranged on the guide rail support frame (111), and the pipetting component (101) and the sliding clamping piece (1022) can move along the slide rail (112).

13. The intelligent cell pipetting workstation of claim 12 wherein: the pipetting assembly (101) comprises a pipetting transverse sliding guide rail piece (10101), a pipetting vertical guide rail piece (10102), a second slider (10103), a pipetting driving mechanism (10104) and a pipetting gun opposite interface (10105);

The liquid-transferring transverse sliding guide rail piece (10101) is in sliding connection with the sliding rail (112), the liquid-transferring vertical guide rail piece (10102) is in sliding connection with the liquid-transferring transverse sliding guide rail piece (10101), the second sliding block (10103) is in vertical sliding connection with the liquid-transferring vertical guide rail piece (10102), and the second sliding block (10103) is connected with the liquid-transferring driving mechanism (10104).

14. The intelligent cell pipetting workstation of claim 7 wherein: the sliding clamping member (1022) comprises a clamping transverse sliding guide rail member (102201), a clamping first vertical guide rail (102202), a first driving member (102203), a first sliding block (102204), a clamping second vertical guide rail (102205), a second driving member (102207), a first clamping part (102208), a sliding rail (112) and a first connecting plate (102209);

The clamping transverse sliding guide rail piece (102201) is in sliding connection with the sliding rail (112), the clamping first vertical guide rail (102202) is in sliding connection with the clamping transverse sliding guide rail piece (102201), a first driving piece (102203) is arranged on the clamping first vertical guide rail (102202), the first driving piece (102203) can drive the first sliding block (102204) to vertically slide along the clamping first vertical guide rail (102202), the clamping second vertical guide rail (102205) is connected with the first sliding block (102204), the first connecting plate (102209) is in vertical sliding connection with the second vertical guide rail (102205), a second driving piece (102207) is arranged on the first connecting plate (102209), and the second driving piece (102207) is connected with the first clamping portion (102208).

15. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the upper operation station (1) further comprises a centrifugal tube cell collection assembly (113), and the centrifugal tube cell collection assembly (113) can collect cells after centrifugation of the centrifugal tube (10405).

16. The intelligent cell pipetting workstation of claim 15 wherein: the centrifuge tube cell collection assembly (113) comprises a centrifuge funnel fixing seat (11301), a funnel driving piece (11302), a funnel supporting guide rail (11303), a funnel sliding block (11304), a funnel (11305) and a fixed storage table (11306);

The centrifugal funnel fixing base (11301) upper end with funnel driving piece (11302) is connected, the upper end of funnel driving piece (11302) is provided with funnel supporting rail (11303), funnel driving piece (11302) can drive funnel slider (11304) slides along funnel supporting rail (11303), the front end of funnel slider (11304) is provided with funnel (11305), the below of funnel (11305) is provided with fixed depositing platform (11306), be provided with centrifuging tube (10405) in fixed depositing platform (11306).

17. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the centrifugal assembly (6) comprises a centrifugal machine (61) and a centrifugal machine supporting frame (62); the centrifuge support frame (62) supports the centrifuge (61);

The centrifuge (61) comprises a centrifuge housing (6102), a centrifuge driver (6103), a centrifuge rotor (6104), a centrifuge rotor (6105), a centrifuge bowl (6106), a first photosensor (6107), and a second photosensor (6108);

Be provided with centrifugal carousel (6104) in centrifugal shell (6102), centrifugal pivot (6105), centrifugal groove (6106), first photoelectric sensor (6107) to and second photoelectric sensor (6108), centrifugal driver (6103) can drive centrifugal carousel (6104) and centrifugal pivot (6105) rotate, the top of centrifugal pivot (6105) sets up a set of centrifugal groove (6106) at least, can place centrifuging tube (10405) in centrifugal groove (6106), the upper end of centrifugal shell (6102) has the opening, and the opening with go up operation station (1) intercommunication.

18. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the side of material loading subassembly (2) is provided with material loading door (3).

19. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the feeding assembly (2) comprises a feeding moving base (201), a feeding driving piece (202), a moving track (203), a feeding moving plate (204), a feeding rotary disc driving piece (205), a feeding rotary disc (206) and a bottle storage hole site (10303);

The feeding device is characterized in that a feeding driving piece (202) is arranged on the feeding moving base (201), a moving track (203) is arranged at the upper end of the feeding driving piece (202), the feeding driving piece (202) can drive the feeding moving plate (204) to slide along the moving track (203), the upper end of the feeding moving plate (204) is connected with a feeding turntable driving piece (205), the feeding turntable driving piece (205) can drive the feeding turntable (206) to rotate, at least one group of storage bottle hole sites (10303) are arranged on the feeding turntable (206), and feeding culture tubes (208) are placed in the storage bottle hole sites (10303).

20. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the upper end of the upper operation station (1) is provided with a laminar flow hood (4).

21. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the upper operation station (1) further comprises a pipetting head assembly (105) and a balancing weight assembly (114);

A balancing weight component (114) is arranged on the lateral side of the pipetting head component (105).

22. The intelligent cell pipetting workstation of claim 21 wherein: the pipetting head assembly (105) comprises a pipetting support (10501) and a pipetting head (10502);

At least one group of pipetting heads (10502) is arranged on the pipetting support frame (10501).

23. The intelligent cell pipetting workstation of claim 21 wherein: the balancing weight assembly (114) comprises a balancing weight supporting frame (11401) and a balancing weight (11402) arranged on the balancing weight supporting frame (11401), wherein at least one group of balancing weights (11402) are arranged on the balancing weight supporting frame (11401).

24. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the upper operation station (1) also comprises a waste liquid and waste material collecting mechanism (115), and the waste liquid and waste material collecting mechanism (115) is used for collecting waste water or waste materials.

25. The intelligent cell pipetting workstation of claim 24 wherein: the waste liquid and waste collection mechanism (115) comprises a first waste liquid collection port (11501), a second waste liquid collection port (11502), a waste material opposite port (11503), a waste liquid storage tank (11504), and a waste material storage tank (11505);

The first waste liquid collecting port (11501), the second waste liquid collecting port (11502) and the waste material pair interface (11503) are all arranged in the upper operation station (1), the waste liquid storage tank (11504) and the waste material storage tank (11505) are arranged below the upper operation station (1), the first waste liquid collecting port (11501) and the second waste liquid collecting port (11502) are discharged into the waste liquid storage tank (11504) through a pipeline, and the waste material pair interface (11503) is discharged into the waste material storage tank (11505) through a pipeline.

26. The intelligent cell pipetting workstation of claim 1 or 2 wherein: the two side walls of the upper operation station (1) are respectively provided with a front door overturning mechanism (116) and a rear sealing door mechanism (117).