CN221431942U - Closed-loop optogenetic stimulation system for animal behavior intervention - Google Patents
Closed-loop optogenetic stimulation system for animal behavior intervention Download PDFInfo
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- CN221431942U CN221431942U CN202322696636.1U CN202322696636U CN221431942U CN 221431942 U CN221431942 U CN 221431942U CN 202322696636 U CN202322696636 U CN 202322696636U CN 221431942 U CN221431942 U CN 221431942U
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Abstract
The utility model relates to the technical field of optogenetic equipment, in particular to a closed-loop optogenetic stimulation system for animal behavior intervention, which comprises the following components: the behavior experiment device is used for placing a target animal; the camera is arranged above the behavior experiment device and is used for acquiring tracking image data of the target animal; the controller is connected with the camera and used for outputting a light stimulation mode according to the tracking image data of the target animal acquired by the camera; the input end of the optogenetic stimulation device is connected with the controller, the output end of the optogenetic stimulation device is connected with a preset brain region of the target animal through an optical fiber, the optogenetic stimulation device is used for carrying out optical stimulation on the preset brain region of the target animal according to an optical stimulation mode output by the controller, and devices such as an electrode and the like are not required to be additionally implanted into the target animal, so that the closed-loop optogenetic stimulation system which is simple and easy to use, reduces the influence of various interferences and can realize animal behavior intervention of long-time intervention and monitoring is provided.
Description
Technical Field
The utility model relates to the technical field of optogenetic equipment, in particular to a closed-loop optogenetic stimulation system for animal behavior intervention.
Background
Deep brain stimulation (deep brain stimulation, DBS) has been used clinically for decades to treat related diseases of the nervous system. But brain states are unpredictable and there are large differences between individuals, for which a technique combining closed-loop optogenetics with deep brain stimulation has been developed. Closed-loop optogenetics is one of the optogenetic techniques used to control and regulate the neural activity or cellular function of an organism. The basic principle of closed-loop optogenetics is to connect a photoreceptive protein with an intracellular signal pathway, and activate or inhibit a specific intracellular signal pathway by stimulating the activity of the photoreceptive protein with light. Closed loop optogenetic techniques can be used to study the function and behavior of the nervous system, for example to study its role in behavior by controlling the activity of specific neurons, and it can also be used to improve and treat related diseases of the nervous system, such as hyperactivity, parkinson, anxiety, depression, and the like. The combination of closed loop optogenetics with deep brain stimulation is a more accurate and efficient therapeutic strategy that uses continuous feedback to update the stimulation protocol, avoiding excessive stimulation therapy. The synchronicity of neural activity is exploited in closed loop optogenetics to determine in real time how and when to perform optogenetics stimulation by monitoring index-guided stimulation in a closed loop feedback loop.
At present, most of the existing closed-loop optogenetic stimulation devices are used for implanting electrodes into brain tissues, electrode data is used as feedback signals, the feedback signals are converted into pulse stimulation output of experimental animals by adopting a complex processing circuit so as to realize closed-loop regulation of the optical stimulation, and researches on other ways for realizing the closed-loop regulation of the optical stimulation are less. However, the data acquisition and processing of the electrical signals are complex, a complex processing circuit is required, and the electrical signals are susceptible to various noises and interferences, such as electromagnetic interference, interference of behaviors such as animal movement, or cross interference of neuron activities. At the same time, prolonged use of electrodes implanted in brain tissue may result in drift or displacement between the electrodes and the brain tissue, resulting in reduced accuracy of the feedback signal.
Disclosure of utility model
Aiming at the technical problems, the utility model provides a closed-loop optogenetic stimulation system for animal behavior intervention, creatively combines the optogenetic technology with the animal behavior principle, and aims to reduce the complexity of a closed-loop optogenetic stimulation device and lighten the influence of various interferences.
The utility model adopts the following technical scheme: a closed loop optogenetic stimulation system for animal behavioral intervention comprising:
the behavior experiment device is used for placing a target animal;
The camera is arranged above the behavior experiment device and is used for acquiring tracking image data of the target animal;
The controller is connected with the camera and used for outputting a light stimulation mode according to the tracking image data of the target animal acquired by the camera;
The input end of the optogenetic stimulation device is connected with the controller, the output end of the optogenetic stimulation device is connected with a preset brain region of the target animal through an optical fiber, and the optogenetic stimulation device is used for performing optical stimulation on the preset brain region of the target animal according to an optical stimulation mode output by the controller.
Among them, the behavior experiment device is a common means for disease model research and clinical disease treatment, and most animal behavior experiments have recognized standards, for example, in an overhead maze experiment device, the activity time of an open arm and a closed arm reflects the conflict between the preference of an animal for a protection area and the inherent motivation for exploring a new environment, and if the animal is in the closed arm for a long time, the animal is generally expressed to generate a higher degree of fear; if the animal is in the open arm for a long period of time, it may be an overactive manifestation. The behavior experiment device can also adopt devices commonly used in animal behavior experiments such as open field, water maze and the like.
In the utility model, a closed feedback loop is formed between the camera, the controller, the optogenetic stimulation device and the target animal in the behavior experiment device. The utility model combines the optogenetic technology with the animal behavior theory, utilizes the synchronism of animal behaviors, namely animal individuals or groups show behavior patterns similar to or coordinated with neural activities under specific environments or situations, realizes the output of optical stimulation according to the tracking image data of the animal behaviors in a closed-loop feedback loop, does not need to additionally implant devices such as electrodes and the like into target animals, and provides a closed-loop optogenetic stimulation system which is simple and easy to use, reduces the influence of various interferences and can realize the intervention of animal behaviors for a long time and monitoring.
Preferably, the closed-loop optogenetic stimulation system comprises a digital converter, wherein the input end of the digital converter is connected with the controller, the output end of the digital converter is connected with the input end of the optogenetic stimulation device, and the digital converter is used for converting the light stimulation mode output by the controller into a corresponding modulation pattern, and the modulation pattern is used for controlling the light orientation;
The optogenetic stimulation device is used for performing optical stimulation on a preset brain region of the target animal according to the modulation pattern input by the digital converter.
Preferably, the optogenetic stimulation device comprises a light source, a light stimulation imaging component and an integrated optical fiber device, wherein the light stimulation imaging component is used for adjusting the reflecting direction of light according to a modulation pattern input by the digital converter and imaging the reflected light at the incident end of the integrated optical fiber device, and the emergent end of the integrated optical fiber device is connected with a preset brain region of a target animal.
Preferably, the optical stimulus imaging component comprises a micro lens array, the micro lens array is composed of a plurality of rotatable micro mirrors, pixels in the modulation pattern are in one-to-one correspondence with the plurality of rotatable micro mirrors, and the micro lens array is used for controlling the rotation angles of the plurality of rotatable micro mirrors according to the modulation pattern.
Preferably, the optical stimulus imaging assembly comprises a beam expansion collimation module, and the beam expansion collimation module is arranged between the light emitting end of the light source and the micro lens array.
Preferably, the optical stimulus imaging assembly comprises a beam expansion module and a micro module, wherein the micro module is connected with the incident end of the integrated optical fiber device, the beam expansion module is arranged between the micro lens array and the micro module, and the back focal plane of the beam expansion module is conjugated with the optical reflecting surface of the micro lens array.
Preferably, the beam expanding module comprises a first double-cemented lens, a second double-cemented lens and an adjustable pinhole diaphragm, wherein the back focal plane of the second double-cemented lens is conjugated with the optical reflecting surface of the micro-lens array, the first double-cemented lens is arranged between the micro-lens array and the second double-cemented lens, and the adjustable pinhole diaphragm is arranged between the first double-cemented lens and the second double-cemented lens.
Preferably, the closed-loop optogenetic stimulation system comprises a supporting frame, a supporting surface is arranged on the supporting frame, the optogenetic stimulation device is arranged on the supporting surface, the camera is fixedly arranged on the lower bottom surface of the supporting surface, and the behavior experiment device is arranged below the supporting surface.
Preferably, the closed-loop optogenetic stimulation system comprises a box body, a supporting plate is arranged in the middle of the box body, the optogenetic stimulation device is arranged on the supporting plate, the camera is fixedly arranged on the lower bottom surface of the supporting plate, and the behavior experiment device is arranged below the supporting plate.
The beneficial technical effects of the utility model at least comprise: the closed-loop optogenetic stimulation system for animal behavior intervention is characterized in that an optogenetic technology is combined with an animal behavior principle, synchronization of animal behaviors, namely animal individuals or groups show behavior patterns similar to or coordinated with neural activities under specific environments or situations, execution of the optostimulation is guided through tracking image data of the animal behaviors in a closed-loop feedback loop, devices such as electrodes and the like are not needed to be additionally implanted into a target animal, and the closed-loop optogenetic stimulation system for animal behavior intervention is simple and easy to use, reduces influence of various interferences and can realize long-time intervention and monitoring.
Other features and advantages of the present utility model will be disclosed in the following detailed description of the utility model and the accompanying drawings.
Drawings
The utility model is further described with reference to the accompanying drawings:
FIG. 1 is a schematic diagram of a closed-loop optogenetic stimulation system for animal behavioral intervention in accordance with an embodiment of the present utility model.
FIG. 2 is a schematic diagram of a closed-loop optogenetic stimulation system for animal behavioral intervention in accordance with an embodiment of the present utility model.
FIG. 3 is a schematic structural diagram of an optogenetic stimulation device according to an embodiment of the utility model.
Fig. 4a and 4b are schematic diagrams of modulation patterns and focusing imaging of the modulation patterns at the incident end of the integrated optical fiber device according to the embodiment of the present utility model.
FIG. 5 is a schematic diagram of a closed-loop optogenetic stimulation system for animal behavioral intervention in accordance with an embodiment of the utility model.
Detailed Description
The technical solutions of the embodiments of the present utility model will be explained and illustrated below with reference to the drawings of the embodiments of the present utility model, but the following embodiments are only preferred embodiments of the present utility model, and not all embodiments. Based on the examples in the implementation manner, other examples obtained by a person skilled in the art without making creative efforts fall within the protection scope of the present utility model.
In the following description, directional or positional relationships such as the terms "inner", "outer", "upper", "lower", "left", "right", etc., are presented for convenience in describing the embodiments and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the utility model.
An embodiment of the present application provides a closed-loop optogenetic stimulation system for animal behavioral intervention, referring to fig. 1, including:
the behavior experiment device 1 is used for placing a target animal;
A camera 2 disposed above the behavior experiment device 1 and used for acquiring tracking image data of the target animal;
A controller 3 connected to the camera 2 and configured to output a light stimulation pattern based on the tracked image data of the target animal acquired by the camera 2;
The input end of the optogenetic stimulation device 5 is connected with the controller 3, the output end of the optogenetic stimulation device 5 is connected with a preset brain region of the target animal through an optical fiber, and the optogenetic stimulation device 5 is used for performing optical stimulation on the preset brain region of the target animal according to an optical stimulation mode output by the controller 3.
Among them, the behavior experiment device 1 is a common means for disease model research and clinical disease treatment, and most animal behavior experiments have recognized standards, for example, in an overhead maze experiment device, the activity time of an open arm and a closed arm reflects the conflict between the preference of an animal for a protection area and the inherent motivation for exploring a new environment, and if the animal is in the closed arm for a long time, the animal is generally expressed to generate a higher degree of fear; if the animal is in the open arm for a long period of time, it may be an overactive manifestation. The behavior experiment device 1 can also adopt devices commonly used in animal behavior experiments such as open field, water maze and the like.
The camera 2 may be a normal camera 2, which is used for acquiring tracking image data of the target animal under the condition of sufficient light, or may be an infrared camera 2, which is used for acquiring tracking image data of the target animal under the dark condition, so long as acquisition of tracking image data of the target animal under different animal behavior experiment conditions can be realized, and position tracking is performed on the target animal, which is not limited in this implementation.
The use method of the output end of the optogenetic stimulation device 5 connected with the preset brain area of the target animal through the optical fiber comprises the following steps: the appropriate photoprotein, such as a photoinduced ion channel (e.g., channel rhodopsin) or a photoprotein modulator (e.g., optogenetic Switch), is selected, and the fiber is positioned to correspond to the target animal's pre-set brain region and is firmly anchored to the target animal's skull to ensure stable light stimulation. The optogenetic stimulation Device 5 may be a Fiber-Coupled Device (Fiber-Coupled Device) that couples the light beam of the light source 501 into an optical Fiber and transmits it to a preset brain region of the target animal for optical stimulation. The optogenetic stimulation device 5 may also be a device comprising a light source 501 and a light stimulation controller, the light source 501 may be a laser or LED for generating light of a suitable wavelength, the light stimulation controller may be a computer or other device for controlling the brightness, frequency and duration of the light source 501, and setting the appropriate light stimulation parameters according to the experimental requirements, and sending an appropriate light stimulation signal to activate or inhibit the light sensitive proteins in the preset brain area of the target animal.
In this embodiment, a closed feedback loop is formed between the camera 2, the controller 3, the optogenetic stimulation device 5 and the target animal in the behavioural experimental device 1. The embodiment combines the optogenetic technology with the animal behavioral principle, utilizes the synchronism of animal behaviors, namely animal individuals or groups show behavior patterns similar to or coordinated with neural activities under specific environments or situations, guides the execution of optical stimulation through tracking image data of animal behaviors in a closed loop feedback loop, does not need to implant devices such as electrodes and the like into target animals, and provides a closed loop optogenetic stimulation system which is simple and easy to use, reduces the influence of various interferences and can realize animal behavior intervention of long-time intervention and monitoring.
In one embodiment of the present disclosure, referring to fig. 2, the closed-loop optogenetic stimulation system includes a digitizer 4, an input end of the digitizer 4 is connected to the controller 3, an output end of the digitizer 4 is connected to an input end of the optogenetic stimulation device 5, and the digitizer 4 is configured to convert the optical stimulation pattern output by the controller 3 into a corresponding modulation pattern, where the modulation pattern is used to control the light direction;
The optogenetic stimulation device 5 is used for performing optical stimulation on a preset brain region of the target animal according to the modulation pattern input by the digital converter 4.
The optogenetic stimulation device 5 may be a grating projector (SPATIAL LIGHT Modulator), which divides the light beam into different areas by means of an incoming modulation pattern and transmits the light beam to a predetermined brain region of the target animal by means of an optical fiber. The optogenetic stimulation device 5 may also be an Optical scanning system (Optical SCANNING SYSTEM), a fiber bundle shaper (Fiber Bundle Shaper), or the like, which is not limited in this embodiment.
By using the digitizer 4 to generate a modulation pattern corresponding to the stimulation pattern and then input the modulation pattern into the optogenetic stimulation device 5, accurate splitting and directing of the light beam can be achieved, thereby more accurately controlling the position and pattern of the optical stimulation. Moreover, the modulation pattern converted by the digital converter 4 can flexibly adjust the stimulation area, the shape and the mode according to experimental requirements, and in particular, different optical patterns can be generated through programming so as to adapt to different experimental designs and research targets.
In one embodiment of the present disclosure, referring to fig. 3, the optogenetic stimulation apparatus 5 includes a light source 501, a light stimulus imaging component and an integrated optical fiber device 502, where the light stimulus imaging component is configured to adjust a reflection direction of light according to a modulation pattern input by the digitizer 4 and image the reflected light on an incident end of the integrated optical fiber device 502, and an exit end of the integrated optical fiber device 502 is connected to a preset brain area of a target animal, so as to implement optical fiber light output coupled to the incident end of the integrated optical fiber device 502 and corresponding to the preset brain area of the target animal.
The light source 501 may be a laser, an LED, a fluorescent lamp, or the like. Preferably, the present embodiment employs 473 nm lasers as the light sources 501. A laser is a special light source 501 that is capable of producing a highly focused, high intensity, monochromatic, coherent beam of light, converting energy into light energy by the process of stimulated radiation, and lasing in an optical resonant cavity.
It can be understood that the integrated optical fiber device 502 is composed of a plurality of optical fibers, the incident ends of the plurality of optical fibers are integrally coupled at one end, and the emergent ends of the plurality of optical fibers are connected with a preset brain region of the target animal, so as to realize multi-channel closed-loop optical stimulation.
The optical stimulus imaging component may be a Liquid crystal spatial light Modulator (lclm), which is an optical device based on Liquid crystal technology, and the refractive index distribution of the Liquid crystal may be adjusted according to an input signal, so as to implement phase modulation of light, and by loading a specific modulation pattern on the Liquid crystal spatial light Modulator, control of the shape and direction of a light beam may be implemented.
Compared with the prior art that a complex circuit is adopted to process electrode data to control a pulse stimulator to output light stimulation, the light stimulation imaging component is adopted to adjust light reflection and imaging based on a modulation pattern input by the digital converter, so that the optical fiber light emitting of the integrated optical fiber device 502 is realized to output light stimulation, the complex circuit and the use of the controller 3 can be reduced, the hardware configuration is simplified, and high-precision optical fiber light emitting and stimulation imaging can be realized through light reflection and imaging by virtue of the light stimulation imaging component, so that accurate positioning and fixed-point stimulation on a preset brain region are realized. Moreover, compared to light pulse stimulation, the use of reflected light for stimulation can reduce energy loss, in particular, the light can maintain higher energy efficiency during reflection and imaging, and can minimize energy loss, thereby improving the efficiency and sustainability of light stimulation to some extent.
In one embodiment of the present disclosure, referring to fig. 3, the optical stimulus imaging assembly includes a microlens array 512, where the microlens array 512 is composed of a plurality of rotatable micromirrors, the pixels in the modulation pattern are in one-to-one correspondence with the plurality of rotatable micromirrors, and the microlens array 512 is used for controlling the rotation angles of the plurality of rotatable micromirrors according to the modulation pattern so as to adjust the reflection direction of the light.
Wherein the micro lens array 512 is an optical device based on micro lens technology, and the rotation angle of the micro mirror can be controlled to control the reflection direction of light by loading a modulation pattern on the micro lens array 512, so as to realize the orientation and modulation of light. In terms of light stimulation, the microlens array 512 can be used to generate complex spatial light patterns for precise stimulation of specific neurons or cells in optogenetic or light stimulation experiments. Specifically, when light is irradiated onto the microlens array 512, the light can be selectively reflected to a specific direction, or reflected to other directions or completely turned off by controlling the rotation angle of the micro mirror. Alternatively, the rotation angle of several rotatable micro-mirrors in the micro-lens array 512 may be controlled by the following method:
1. Electronic control: each micromirror may be connected to a separate electronic control unit for controlling its rotation angle by transmitting electrical signals corresponding to the pixels in the modulation pattern. This can be accomplished by using electronics (such as simple drive circuitry and an electronic signal generator) to send a specific voltage or current signal to each micromirror to control its rotation.
2. Optical control: the optical signal is used to control the micromirror rotation angle in the microlens array 512. An optical element (e.g., an optical fiber) or a modulation pattern may be used to transmit a particular mode or beam onto the micro-mirror, thereby activating its rotation.
3. Mechanical control: the rotation angle of the micro-mirrors in the micro-lens array 512 is controlled by mechanical means such as a simple driver, motor or micro-actuator.
Illustratively, the microlens array 512 in this embodiment is used in the following manner: assuming that the 473 nm laser emits 24 ° of incident light, each micromirror cell has three stable states: +12° or +10° (on), -12° or-10 ° (off). It will be appreciated that loading different modulation patterns on microlens array 512 will result in different spots focused on the incident end of integrated fiber device 502. Specifically, the pixels in the modulation pattern are in one-to-one correspondence with the rotatable micromirrors, and the "black pixels" and "white pixels" in the modulation pattern may represent binary "1" and "0" states of the micromirror control signals, that is, the "on" and "off" states of the micromirrors respectively. The "black pixel" in the modulation pattern corresponds to a micro-mirror "on", and the micro-mirror deflects +12 degrees or +10 degrees based on the equilibrium position, and 24 ° of incident light is reflected and focused on the incident end of the integrated optical fiber device 502 along the optical axis direction, so as to form a bright light spot. The "white pixel" in the modulation pattern corresponds to one micro-mirror "off" and then the micro-mirror deflects-12 degrees or-10 degrees based on the equilibrium position, and the 24 incident light will not be focused on the incident end of the integrated fiber device 502 and therefore will appear as a dark pixel on the incident end of the integrated fiber device 502.
For example, a modulation pattern corresponding to one optical stimulus mode is loaded on the micro lens array 512, focused on the incident end of the integrated optical fiber device 502 to form two bright light spots, i.e. two optical stimulus points, corresponding to two optical fibers for emitting light, please refer to fig. 4a, another modulation pattern corresponding to another optical stimulus mode is loaded on the micro lens array 512, focused on the incident end of the integrated optical fiber device 502 to form one bright light spot, i.e. one optical stimulus point, corresponding to one optical fiber for emitting light, please refer to fig. 4b. Of course, if all the modulation patterns are "white pixels", all the micromirrors are in "off" state, and a completely dark image is displayed on the incident end of the integrated optical fiber device 502, which indicates that there is no light stimulation point, i.e. no light is emitted from the optical fiber, so that the current light stimulation mode is behavior intervention that does not perform light stimulation on the mouse.
In one embodiment of the present disclosure, referring to fig. 3, the optical stimulus imaging assembly includes a beam expansion collimating module 511, where the beam expansion collimating module 511 is disposed between the light emitting end of the light source 501 and the microlens array 512.
Alternatively, the expanded beam collimation module 511 may be the following:
1. Lens (Lens): lenses are one of the most common beam expanding and collimating devices, and by choosing the proper focal length and position of the lenses, the effect of expanding and collimating the beam from the light source 501 can be achieved.
2. Cylindrical lens (CYLINDRICAL LENS): the cylindrical lens is a lens of a special shape, and has a smaller radius of curvature in one direction and a larger radius of curvature in the other direction. A cylindrical lens may be used to expand the beam in one direction to achieve collimation.
3. Waveguide (Waveguide): a waveguide is a light guiding structure that can transmit a light beam from a light source 501 to a microlens array 512. The waveguide itself may have a specific geometry to achieve beam expansion and collimation of the light beam.
The beam expansion and collimation assembly used in this embodiment can diffuse the light beam emitted by the light source 501 to have a larger transverse dimension, which is helpful to cover a larger microlens array 512 area or increase the light intensity distribution of the light beam, and the beam expansion and collimation assembly can adjust and control the directionality and parallelism of the light beam, which is helpful to concentrate the light beam to the microlens array 512 area, and improve the focusing effect and transmission efficiency of the light beam. In summary, the beam spreading and collimating component spreads and collimates the light beam, so that the light of the light source 501 can be more uniformly distributed on the microlens array 512, and the problems of hot spots or uneven light intensity are avoided.
In one embodiment of the present disclosure, referring to fig. 3, the optical stimulus imaging assembly includes a beam expander module 513 and a micro module 514, the micro module 514 is connected to the incident end of the integrated optical fiber device 502, the beam expander module 513 is disposed between the micro module 514 and the micro lens array 512, and a back focal plane of the beam expander module 513 is conjugated to an optical reflecting surface of the micro lens array 512.
Wherein conjugation is an optical term used to describe the propagation of light in an optical system. When two optical elements or surfaces are conjugate, this means that light rays, after exiting one element or surface, will pass through to be focused onto the other element or surface. Wherein, since the beam expansion module 513 is disposed between the microlens array 512 and the micro module 514, it is understood that the optical reflection surface of the microlens array 512 coincides with the front focal surface of the beam expansion module 513, and the back focal surface of the beam expansion module 513 coincides with the front focal surface of the micro module 514. The micro-module may be a device composed of a lens and an objective lens, which is one of the common micro-devices, and focusing and adjusting of the reflected light may be achieved by selecting a proper focal distance and position between the lens and the objective lens.
It is understood that the optically reflective surface of microlens array 512 is the reflective surface when the micromirror is in the "on" state.
In this embodiment, after the light is reflected by the microlens array 512, the reflected light can be focused and adjusted by the diffusion of the beam expanding module 513 and the refraction and focusing of the micro module 514, so that the reflected light can enter the incident end of the integrated optical fiber device 502 at a proper position and angle to ensure effective optical coupling, form a final optical stimulation point, and make the corresponding optical fiber emit light to realize optical stimulation.
In one embodiment of the present disclosure, referring to fig. 3, the beam expanding module 513 includes a first doublet 521, a second doublet 523, and an adjustable pinhole diaphragm 522, the back focal plane of the second doublet 523 is conjugate with the optical reflection plane of the microlens array 512, the first doublet 521 is disposed between the microlens array 512 and the second doublet 523, and the adjustable pinhole diaphragm 522 is disposed between the first doublet 521 and the second doublet 523.
Wherein, the double cemented lens refers to an optical element formed by two lenses cemented together. The front focal plane of the first doublet 521 refers to the surface of the first doublet 521 facing the microlens array 512, and the front focal plane of the first doublet 521 coincides with the optical reflection surface of the microlens array 512. By selecting the appropriate focal length and position of the first and second doublet lenses 521 and 523, a post-expansion effect of reflecting the light beam from the microlens array 512 can be achieved.
Each micromirror in the microlens array 512 diffracts and focuses incident light, and the primary imaging light is focused through the microlens array 512 onto the front focal plane of the micromodule 514 to form an image that is refocused onto the incident end of the integrated fiber device 502. However, due to interference effects between the periodic structure of the microlens array 512 and the micromirrors, some additional diffracted light, i.e., reflected light of the excessive diffraction orders of the microlens array 512, may be generated, which may interfere with or degrade the quality and sharpness of the image, in particular, may cause blurring, halation, or other distortion of the image. Therefore, in order to reduce or block the reflected light of these unwanted diffraction orders, the present embodiment provides an adjustable pinhole diaphragm 522 between the first doublet 521 and the second doublet 523.
In one embodiment of the present disclosure, referring to fig. 1, the closed-loop optogenetic stimulation system includes a support frame 6, a support surface is disposed on the support frame 6, an optogenetic stimulation device 5 is disposed on the support surface, a camera is fixedly mounted on a lower bottom surface of the support surface, and a behavior experiment device 1 is disposed below the support surface.
The optogenetic stimulation device 5 is arranged above the behavior experiment device 1 through the supporting frame 6, so that the influence of the weight of the optical fiber connected with the brain of the target animal on the free movement of the target animal is reduced to a certain extent, and the phenomenon of optical fiber winding can be reduced.
In one embodiment of the present disclosure, referring to fig. 5, the closed-loop optogenetic stimulation system includes a case 7, a support plate 701 is disposed in the middle of the case 7, the optogenetic stimulation device 5 is disposed on the support plate 701, a camera is fixedly installed on the lower bottom surface of the support plate 701, and the behavior experiment device 1 is disposed under the support plate 701.
It will be appreciated that a gap or through hole is provided between the support plate 701 and the side of the housing 7 for passing the optical fibre. The closed-loop optogenetic stimulation system for animal behavior intervention provided by the embodiment can be used for animal behavior interference experiments in dark environments while achieving the effects of the embodiment, so that the application range of the system is expanded to a certain extent.
While the utility model has been described in terms of embodiments, it will be appreciated by those skilled in the art that the utility model is not limited thereto but rather includes the drawings and the description of the embodiments above. Any modifications which do not depart from the functional and structural principles of the present utility model are intended to be included within the scope of the appended claims.
Claims (9)
1. A closed loop optogenetic stimulation system for animal behavioral intervention comprising:
the behavior experiment device is used for placing a target animal;
The camera is arranged above the behavior experiment device and is used for acquiring tracking image data of the target animal;
The controller is connected with the camera and used for outputting a light stimulation mode according to the tracking image data of the target animal acquired by the camera;
The input end of the optogenetic stimulation device is connected with the controller, the output end of the optogenetic stimulation device is connected with a preset brain region of the target animal through an optical fiber, and the optogenetic stimulation device is used for performing optical stimulation on the preset brain region of the target animal according to an optical stimulation mode output by the controller.
2. A closed loop optogenetic stimulation system for animal behavioral intervention of claim 1 wherein,
The closed-loop optogenetic stimulation system comprises a digital converter, wherein the input end of the digital converter is connected with the controller, the output end of the digital converter is connected with the input end of the optogenetic stimulation device, the digital converter is used for converting the optical stimulation mode output by the controller into a corresponding modulation pattern, and the modulation pattern is used for controlling the orientation of light;
The optogenetic stimulation device is used for performing optical stimulation on a preset brain region of the target animal according to the modulation pattern input by the digital converter.
3. A closed loop optogenetic stimulation system for animal behavioral intervention of claim 2 wherein,
The light genetic stimulation device comprises a light source, a light stimulation imaging component and an integrated optical fiber device, wherein the light stimulation imaging component is used for adjusting the reflecting direction of light according to a modulation pattern input by the digital converter and imaging the reflected light at the incident end of the integrated optical fiber device, and the emergent end of the integrated optical fiber device is connected with a preset brain region of a target animal.
4. A closed loop optogenetic stimulation system for animal behavioral intervention of claim 3 wherein,
The optical stimulus imaging component comprises a micro lens array, wherein the micro lens array is composed of a plurality of rotatable micro reflectors, pixels in the modulation pattern are in one-to-one correspondence with the plurality of rotatable micro reflectors, and the micro lens array is used for controlling the rotation angles of the plurality of rotatable micro reflectors according to the modulation pattern.
5. A closed loop optogenetic stimulation system of animal behavioral intervention of claim 4 wherein,
The optical stimulus imaging component comprises a beam expansion collimation module, and the beam expansion collimation module is arranged between the light emitting end of the light source and the micro lens array.
6. A closed loop optogenetic stimulation system of animal behavioral intervention of claim 4 wherein,
The optical stimulus imaging assembly comprises a beam expansion module and a micro module, wherein the micro module is connected with the incident end of the integrated optical fiber device, the beam expansion module is arranged between the micro lens array and the micro module, and the back focal plane of the beam expansion module is conjugated with the optical reflecting surface of the micro lens array.
7. A closed loop optogenetic stimulation system of animal behavioral intervention of claim 6 wherein,
The beam expanding module comprises a first double-cemented lens, a second double-cemented lens and an adjustable pinhole diaphragm, wherein the back focal plane of the second double-cemented lens is conjugated with the optical reflecting surface of the micro-lens array, the first double-cemented lens is arranged between the micro-lens array and the second double-cemented lens, and the adjustable pinhole diaphragm is arranged between the first double-cemented lens and the second double-cemented lens.
8. A closed loop optogenetic stimulation system for animal behavioral intervention according to any one of claims 1-7,
The closed-loop optogenetic stimulation system comprises a supporting frame, a supporting surface is arranged on the supporting frame, the optogenetic stimulation device is arranged on the supporting surface, the camera is fixedly arranged on the lower bottom surface of the supporting surface, and the behavior experiment device is arranged below the supporting surface.
9. A closed loop optogenetic stimulation system for animal behavioral intervention according to any one of claims 1-7,
The closed-loop optogenetic stimulation system comprises a box body, a supporting plate is arranged in the middle of the box body, the optogenetic stimulation device is arranged on the supporting plate, the camera is fixedly arranged on the lower bottom surface of the supporting plate, and the behavior experiment device is arranged below the supporting plate.
Priority Applications (1)
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| CN120052830A (en) * | 2025-04-25 | 2025-05-30 | 中国人民解放军西部战区总医院 | Test device for brain science animal experiments based on optogenetic technology |
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