CN221166534U - Purification column capable of simultaneously removing DNA and impurities - Google Patents

Abstract

The utility model discloses a purification column capable of simultaneously removing DNA and impurities, which comprises a centrifuge tube and an adsorption column, wherein the upper end of the centrifuge tube is provided with an opening, the adsorption column is placed in the centrifuge tube, the top of the adsorption column is provided with a top cover, the lower end of the adsorption column is provided with a water outlet, and the inside of the adsorption column at the upper part of the water outlet is sequentially provided with a preparation ring, a filtering membrane and an adsorption membrane from top to bottom. The purification column does not need to separate supernatant liquid by using an organic reagent in the operation process, so that the problem that tissue fragments after centrifugation are carelessly sucked in the extraction process can be solved; in the operation process, DNase is not needed, and the whole kit can be stored and transported at normal temperature, so that the reagent cost is saved; the operation time for extracting RNA is shortened, and the operation is simpler.

Description

Purification column capable of simultaneously removing DNA and impurities

Technical Field

The utility model relates to the technical field of purification columns, in particular to a purification column capable of simultaneously removing DNA and impurities.

Background

The purification column can be used for extracting and purifying nucleic acid of plant, animal, microorganism and other tissue materials, and has simple operation, but in the process of extracting RNA by using the purification column, DNA hydrolase and organic reagent are generally needed, the DNA hydrolase needs to be transported at low temperature, and is inconvenient to assemble in the same kit, and chloroform and phenol which are usually extracted by the organic reagent are toxic and harmful substances, so that the physical health of operators can be endangered.

Disclosure of utility model

Aiming at the defects of the prior art, the utility model aims to provide a purification column capable of removing DNA and impurities simultaneously.

The utility model adopts the following technical scheme to realize the aim:

The purification column capable of simultaneously removing DNA and impurities in the technical scheme comprises a centrifuge tube and an adsorption column, wherein an opening is formed in the upper end of the centrifuge tube, the adsorption column is placed in the centrifuge tube, a top cover is arranged at the top of the adsorption column, a water outlet is formed in the lower end of the adsorption column, and a preparation ring, a filtering membrane and an adsorption membrane are sequentially arranged inside the adsorption column at the upper side of the water outlet from top to bottom.

In the above technical scheme, preferentially, the upper part of the adsorption column is provided with a circle of protrusions larger than the diameter of the adsorption column, the diameter of the protrusions is larger than that of the centrifuge tube, the top of the protrusions is provided with a top cover, and the length of the adsorption column is shorter than that of the centrifuge tube.

In the above technical solution, preferably, the filtering membrane is provided with a layer.

In the above technical solution, preferably, the adsorption film is provided with six layers.

In the above technical solution, preferably, the preparation ring is annular, and three protruding blocks are provided in the ring.

In the above technical solution, preferably, the filtering membrane is a cellulose filter paper or a glass fiber filter paper.

In the above technical solution, preferably, the adsorption film is a silicon matrix film.

In the above technical solution, preferably, the adsorption column between the adsorption film and the water outlet is funnel-shaped.

Compared with the prior art, the purification column capable of simultaneously removing DNA and impurities has the following beneficial effects:

The purification column does not need to separate supernatant liquid by using an organic reagent in the operation process, so that the problem that tissue fragments after centrifugation are carelessly sucked in the extraction process can be solved; in the operation process, DNase is not needed, and the whole kit can be stored and transported at normal temperature, so that the reagent cost is saved; the operation time for extracting RNA is shortened, and the operation is simpler.

Drawings

FIG. 1 is a schematic view of a centrifuge tube of the present utility model;

FIG. 2 is a schematic diagram of an adsorption column of the present utility model;

FIG. 3 is a schematic diagram of the overall structure of the purification column of the present utility model;

FIG. 4 is a schematic view of a preparation circle of the present utility model;

In the figure: 1-centrifuge tube, 2-adsorption column, 201-bulge, 202-top cover, 203-water outlet, 3-preparation ring, 301-bump, 4-filtering membrane, 5, and adsorption membrane.

Detailed Description

It should be noted that the positional or positional relationship indicated by the terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying positive importance. Merely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

The utility model is further illustrated by the following examples in conjunction with the accompanying drawings:

Referring to fig. 1 to 4, the purification column capable of simultaneously removing DNA and impurities provided by the embodiment of the utility model comprises a centrifuge tube 1 and an adsorption column 2, wherein an opening is arranged at the upper end of the centrifuge tube 1, the adsorption column 2 is placed in the centrifuge tube 1 from the opening, a top cover 202 is arranged at the top of the adsorption column 2, a water outlet 203 is arranged at the lower end of the adsorption column 2, a preparation ring 3, a filtering membrane 4 and an adsorption membrane 5 are sequentially arranged in the adsorption column 2 at the upper side of the water outlet 203 from top to bottom, the filtering membrane 4 is provided with a layer, the adsorption membrane 5 is provided with six layers, and the preparation ring 3 is used for fixing the positions of the filtering membrane 4 and the adsorption membrane 5 at the lower side, so that the position movement of the membrane due to the action of centrifugal force in the high-speed centrifugation process is avoided. Meanwhile, the preparation ring 3 also avoids that liquid directly leaks downwards from between the membrane and the side wall of the adsorption column 2 without passing through the filtering membrane 4 and the adsorption membrane 5, so that the removal effect of impurities and DNA in the liquid is poor. Wherein the filtering membrane 4 filters impurities in the liquid, and the adsorption membrane 5 is used for adsorbing DNA.

In order to implement the utility model better, referring to fig. 1, 2 and 3, in one embodiment, the upper part of the adsorption column 2 is provided with a circle of protrusions 201 with a diameter larger than that of the adsorption column 2, the diameter of the protrusions 201 is larger than that of the centrifuge tube 1, the top of the protrusions 201 is provided with a top cover 202, and the length of the adsorption column is shorter than that of the centrifuge tube. The protrusion 201 prevents the adsorption column 2 from falling into the centrifuge tube 1, and enables a distance to exist between the water outlet 203 at the lower end of the adsorption column 2 and the liquid flowing down in the centrifuge tube 1.

In order to better implement the utility model, referring to fig. 4, in one embodiment, the preparation ring 3 is annular, and three protrusions 301 are provided in the ring, and the protrusions 301 further press the filtering membrane 4 and the adsorption membrane 5 on the lower side.

To better practice the utility model, referring to fig. 3, in one embodiment, the filter membrane 4 is a cellulose filter paper or a glass fiber filter paper.

To better practice the utility model, referring to fig. 3, in one embodiment, the adsorption membrane 5 is a silicon matrix membrane.

To better practice the utility model, referring to fig. 2 and 3, in one embodiment, the adsorption column 2 between the adsorption membrane 5 and the water outlet 203 is funnel-shaped.

Based on the purification column capable of simultaneously removing DNA and impurities, the working principle is as follows:

Before sample purification, firstly, a lysate is used for cracking a sample, nucleic acid in the sample is released, an adsorption column 2 is placed into the centrifuge tube 1 from the upper end of the centrifuge tube 1, then the cracked liquid is injected from the upper end of the adsorption column 2, a top cover 202 is closed, then the whole purification column is placed into a centrifuge, centrifugation is carried out at 12000rpm for 2min, after centrifugation is finished, the purification column is taken out, the adsorption column 2 is taken out from the centrifuge tube 1, liquid in the centrifuge tube 1 is left, at the moment, DNA and impurities are trapped in the adsorption column 2 through a filtering membrane 4 and an adsorption membrane 5, then a binding liquid is added into the liquid, the binding liquid is used for providing proper salt ion concentration and pH for the next-step RNA adsorption, the binding liquid is transferred into the RNA adsorption column after being mixed evenly, centrifugation is carried out for 12000rpm again for 1min, waste liquid flowing out of the RNA adsorption column after the RNA adsorption column is removed, deproteinized liquid and deionized liquid are added into the RNA adsorption column for further cleaning the RNA adsorption column, eluent is added onto the RNA adsorption column, centrifugation is carried out again for 12000rpm for 1min, and RNA is obtained after elution.

In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

The above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the scope of the present utility model. It will be apparent that the described embodiments are merely some, but not all, embodiments of the utility model. Based on these embodiments, all other embodiments that may be obtained by one of ordinary skill in the art without inventive effort are within the scope of the utility model. Although the present utility model has been described in detail with reference to the above embodiments, those skilled in the art may still combine, add or delete features of the embodiments of the present utility model or make other adjustments according to circumstances without any conflict, so as to obtain different technical solutions without substantially departing from the spirit of the present utility model, which also falls within the scope of the present utility model.

Claims (8)

1. Can get rid of purification post of DNA and impurity simultaneously, a serial communication port, including centrifuging tube (1) and adsorption column (2), centrifuging tube (1) upper end is equipped with the opening, adsorption column (2) are placed in centrifuging tube (1), adsorption column (2) top is equipped with top cap (202), and the lower extreme is equipped with delivery port (203), inside from last preparation circle (3), filtration membrane (4) and adsorption membrane (5) of being equipped with down in proper order of adsorption column (2) of delivery port (203) upside.

2. The purification column capable of simultaneously removing DNA and impurities according to claim 1, wherein a circle of protrusions (201) larger than the diameter of the adsorption column (2) is arranged on the upper portion of the adsorption column (2), the diameter of each protrusion (201) is larger than that of the centrifuge tube (1), a top cover (202) is arranged on the top of each protrusion (201), and the length of the adsorption column (2) is shorter than that of the centrifuge tube (1).

3. Purification column for simultaneous removal of DNA and impurities according to claim 1, characterized in that the filter membrane (4) is provided with a layer.

4. Purification column for simultaneous removal of DNA and impurities according to claim 1, characterized in that the adsorption membrane (5) is provided with six layers.

5. Purification column for simultaneous removal of DNA and impurities according to claim 1, wherein the preparation ring (3) is ring-shaped and three projections (301) are provided in the ring.

6. Purification column for simultaneous removal of DNA and impurities according to claim 4, characterized in that the filter membrane (4) is a cellulose filter paper or a glass fiber filter paper.

7. Purification column for simultaneous removal of DNA and impurities according to claim 5, characterized in that the adsorption membrane (5) is a silicon matrix membrane.

8. Purification column for simultaneous removal of DNA and impurities according to claim 1, characterized in that the adsorption column (2) between the adsorption membrane (5) and the water outlet (203) is funnel-shaped.