CN219977953U - A simple and easy nitrogen gas weathers device for laboratory LNP preparation - Google Patents
A simple and easy nitrogen gas weathers device for laboratory LNP preparation Download PDFInfo
- Publication number
- CN219977953U CN219977953U CN202321196088.XU CN202321196088U CN219977953U CN 219977953 U CN219977953 U CN 219977953U CN 202321196088 U CN202321196088 U CN 202321196088U CN 219977953 U CN219977953 U CN 219977953U
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- Prior art keywords
- hose
- laboratory
- lnp
- bottle
- nitrogen
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 126
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 229910001873 dinitrogen Inorganic materials 0.000 title description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241001411320 Eriogonum inflatum Species 0.000 claims abstract description 8
- 238000000861 blow drying Methods 0.000 claims abstract description 7
- 238000003825 pressing Methods 0.000 claims description 9
- 238000010521 absorption reaction Methods 0.000 claims description 8
- 238000009413 insulation Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims 1
- 238000007664 blowing Methods 0.000 abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 208000001034 Frostbite Diseases 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model relates to the field of sample preparation equipment for testing, in particular to a simple nitrogen blow-drying device for laboratory LNP manufacture, which comprises a liquid containing bottle for containing liquid nitrogen and a fixing seat for limiting the liquid containing bottle; the fixing seat is provided with a retaining groove for limiting and preserving heat of the liquid bottle; the liquid containing bottle is detachably connected with a bottle stopper, the bottle stopper is connected with a hose which can be inserted into a centrifuge tube containing a sample, and a water absorbing layer is arranged in the hose. The utility model has simple and convenient operation and improves the nitrogen blowing operation speed of the LNP sample.
Description
Technical Field
The utility model relates to the field of sample preparation equipment for testing, in particular to a simple nitrogen blow-drying device for laboratory LNP manufacture.
Background
Lipid Nanoparticles (LNP) are one of the important technologies in lipid carrier drug delivery systems, and LNP is capable of safely and effectively delivering nucleic acids, which has become an important advance in oligonucleotide-based therapeutic drugs. The preparation method of the LNP in the laboratory commonly used at present mainly comprises an injection method (organic reagent such as ethyl alcohol ether) and the like. In the injection method, the organic solvent needs to be removed by low-pressure evaporation, dialysis, nitrogen blowing, or the like. The preparation of small-volume LNP generally adopts nitrogen blowing method, and nitrogen blowing method is to use nitrogen in inert gas to purge at the top of liquid, and the gas-liquid balance above the liquid can be broken through the fast flow of nitrogen, so that the volatilization and concentration speed of liquid such as ethanol and the like is increased, and the purpose of fast concentrating samples is achieved. In each research laboratory, however, only liquid nitrogen for freezing and storing cells is generally provided, and nitrogen cylinders or nitrogen blowers are rarely provided and are expensive.
Based on the above-mentioned problem, publication number CN207894701U discloses a nitrogen blows appearance, including support, nitrogen bottle and the intake pipe that one end and nitrogen bottle are linked together, the intake pipe other end be equipped with a plurality of outlet ducts that are linked together, every outlet duct exit end all be connected with the nitrogen that enables a large amount of nitrogen gas and slowly flow out from the needle mouth and blow the needle, the support on be equipped with the jack that is used for inserting and establish the nitrogen and blow the needle, the jack below be provided with the base that is used for fixed container correspondingly. The method has the characteristics of simplicity, practicability, high efficiency and stability, and is suitable for concentrating samples stored in different experimental apparatuses.
However, the structure of the existing nitrogen blowing instrument is relatively large, because the nitrogen blowing operation needs to be performed on the LNP sample with small volume rapidly in the experimental environment, the existing nitrogen blowing instrument needs to insert the sample tube into the base below the needle opening when taking and placing, after the nitrogen blowing operation is completed, the nitrogen blowing needle is closed first and then taken out from the base, the operation is complex, the use speed is low, if the nitrogen blowing needle is not closed, the distance between the LNP sample and the nitrogen blowing needle is reduced when the sample tube is taken out from the base, the LNP sample with small volume in the sample tube is blown out, and waste is caused.
Disclosure of Invention
The utility model aims to provide a simple nitrogen blow-drying device for laboratory LNP manufacture, which solves the problems of complicated operation and low use speed of the existing device.
The simple nitrogen blow-drying device for laboratory LNP manufacture comprises a liquid bottle for placing liquid nitrogen and a fixing seat for limiting the liquid bottle;
the fixing seat is provided with a retaining groove for limiting and preserving heat of the liquid bottle;
the liquid bottle is characterized in that a bottle stopper is detachably connected to the liquid bottle, a hose capable of being inserted into a centrifuge tube containing a sample is connected to the bottle stopper, and a water absorption layer is arranged in the hose.
Preferably, the hose is provided with a limiting head capable of propping against the pipe orifice of the centrifuge tube, the radial extension length of the limiting head on the hose is larger than the difference value between the diameter of the hose and the diameter of the centrifuge tube, and the length from the limiting head to the end part of the hose inserted into the centrifuge tube is smaller than or equal to the length from the hose inserted into the centrifuge tube.
More preferably, the limit head is integrally formed on a threaded cap which is in threaded engagement with the hose.
Preferably, the depth of the retaining groove is two thirds of the height of the liquid containing bottle, and the thickness of the groove wall of the retaining groove is 5-10cm.
More preferably, the outer side wall of the fixing seat is provided with a thermal insulation layer, and the thickness of the thermal insulation layer is 0.2-1cm.
More preferably, a limiting layer is fixedly arranged on the inner wall of the retaining groove, and the limiting layer is made of fabric.
Preferably, the flexible pipe is provided with an elastic bag in an integrated manner, the elastic bag is provided with an elastic regulating bag in an integrated manner, a pressing ball for shielding the flexible pipe is arranged at the joint of the elastic bag and the air outlet side of the flexible pipe, an elastic rope for tensioning is connected between the pressing ball and the flexible pipe on the air outlet side, and a soft stay rope is connected between the pressing ball and the regulating bag.
More preferably, the pressing ball is internally provided with a cavity, the wall of the cavity is mesh-shaped, and the cavity is filled with moisture-absorbing paper.
Compared with the prior art, the beneficial effect of this scheme is:
when the LNP sample is subjected to nitrogen blowing, liquid nitrogen in the liquid containing bottle is converted into nitrogen at normal temperature and normal pressure, and the nitrogen is introduced into the centrifuge tube through the hose to perform nitrogen blowing operation on the LNP sample. When inserting the hose into the centrifuging tube, because the centrifuging tube size and gravity are all less, only need the one hand hold the centrifuging tube, then another hand inserts the hose into the centrifuging tube and can carry out nitrogen and blow, after accomplishing the operation, directly take away the hose from the centrifuging tube top can, easy operation is convenient, improves the nitrogen of LNP sample and blows operating speed.
Drawings
FIG. 1 is a front view of a first embodiment of a simplified nitrogen blow-dry apparatus for laboratory LNP fabrication in accordance with the present utility model;
FIG. 2 is a radial cross-sectional view of a hose placement limit head in a second embodiment of a simple nitrogen blow-dry apparatus for laboratory LNP fabrication in accordance with the present utility model;
fig. 3 is a cross-sectional view of the elastic bladder in a third embodiment of the simple nitrogen blow-drying apparatus for laboratory LNP manufacturing of the present utility model.
Detailed Description
Further details are provided below with reference to the specific embodiments.
Reference numerals in the drawings of the specification include: fixing base 1, liquid bottle 2, bottle plug 3, hose 4, centrifuging tube 5, screw cap 6, spacing head 7, elastic bag 8, regulation bag 9, stay cord 10, press ball 11, elastic rope 12.
Example 1
A simple nitrogen blow-dry apparatus for laboratory LNP fabrication as shown in fig. 1: the liquid nitrogen filling device comprises a liquid containing bottle 2 for containing liquid nitrogen and a fixing seat 1 for limiting the liquid containing bottle 2, wherein the liquid nitrogen in the liquid containing bottle 2 is filled according to requirements. The outer side wall of the fixing seat 1 is provided with the thermal insulation layer, the thickness of the thermal insulation layer is 0.2-1cm, the thermal insulation layer can be made of cotton cloth, and the thermal insulation layer can prevent frostbite caused by mistakenly touching the fixing seat 1 after the temperature of liquid nitrogen is conducted out.
The fixing base 1 is provided with a retaining groove for limiting and preserving the height of the liquid containing bottle 2, the depth of the retaining groove is two thirds of the height of the liquid containing bottle 2, the thickness of the groove wall of the retaining groove is 5-10cm, the fixing groove can limit the liquid containing bottle 2 and simultaneously reduce frostbite caused by liquid nitrogen temperature conduction when the fixing base 1 is touched by mistake, and the inner wall of the retaining groove is fixedly provided with a limiting layer which is made of fabrics such as cotton cloth.
The bottle stopper 3 is detachably connected to the liquid containing bottle 2, the hose 4 capable of being inserted into the centrifuge tube 5 containing the sample is connected to the bottle stopper 3, a water absorption layer is arranged in the hose 4, water absorption can be carried out by water absorption paper, the water absorption paper is directly inserted into the hose 4, and part of water in nitrogen can be absorbed by the water absorption layer.
The specific implementation process is as follows:
when the LNP sample is nitrogen-blown, liquid nitrogen in the liquid bottle 2 is converted into nitrogen at normal temperature and normal pressure, and the nitrogen is introduced into the centrifuge tube 5 through the hose 4 to perform the nitrogen-blowing operation of the LNP sample. When inserting centrifuging tube 5 with hose 4, because centrifuging tube 5 size and gravity are all less, only need the one hand hold centrifuging tube 5, then the other hand inserts hose 4 into centrifuging tube 5 and can carry out nitrogen and blow, after accomplishing the operation, directly take away hose 4 from centrifuging tube 5 top can, easy operation is convenient, improves the nitrogen of LNP sample and blows operating speed.
Example two
A simple nitrogen blow-drying device for LNP manufacturing in a laboratory is different from the first embodiment in that, as shown in FIG. 2, a limiting head 7 capable of propping against the pipe orifice of a centrifuge pipe 5 is arranged on a hose 4, the limiting head 7 is a fan-shaped piece with a central angle of 30-90 degrees, the extending length of the limiting head 7 in the radial direction of the hose 4 is larger than the difference value between the diameter of the hose 4 and the diameter of the centrifuge pipe 5, and the length from the limiting head 7 to the end part of the hose 4 inserted into the centrifuge pipe 5 is smaller than or equal to the length from the hose 4 inserted into the centrifuge pipe 5.
In another embodiment, the spacing head 7 is integrally formed on the threaded cap 6, and the threaded cap 6 is threadedly engaged with the hose 4. The length of the hose 4 extending into the centrifuge tube 5 is conveniently adjusted.
In order to insert the degree of centrifuging tube 5 on can being suitable for different centrifuging tubes 5 for hose 4, through screwing up spacing head 7 on the hose 4, can adjust the length that lets hose 4 support in centrifuging tube 5 mouth of pipe department when stretching into centrifuging tube 5, can carry out corresponding regulation according to centrifuging tube 5's size, application scope is wider.
Example III
A simple and easy nitrogen gas weathers device for LNP preparation in laboratory, with embodiment one, the difference lies in, as shown in FIG. 3, integrated into one piece has elastic bag 8 on the hose 4, integrated into one piece has elastic regulation bag 9 on the elastic bag 8, elastic bag 8 and regulation bag 9 use by the material that is pressed the back and automatic resilience reset make, regulation bag 9 is located the department that is close to hose 4 air inlet side, the butt ball 11 that shelters from hose 4 is installed to the junction of elastic bag 8 and hose 4 air outlet side, be connected with the elastic rope 12 that is used for taut between butt ball 11 and the hose 4 of air outlet side, be connected with soft stay cord 10 between butt ball 11 and the regulation bag 9, seted up the cavity in the butt ball 11, the chamber wall of cavity is the mesh form, the intussuseption of cavity is filled with the absorbent paper.
The setting of elastic bag 8 can hold and store a certain amount of nitrogen gas to pull the sword through pulling adjustment bag 9 and support and press ball 11, adjust the air output of hose 4, reduce the extravagant automatic nitrogen gas that overflows in the change centrifuging tube 5 in-process, and can also adsorb a small amount of moisture in the nitrogen gas through the moisture absorption paper that supports in pressing ball 11, guarantee the dryness of nitrogen gas.
The foregoing is merely exemplary embodiments of the present utility model, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present utility model, and these should also be considered as the scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the utility of the patent. The protection scope of the present utility model is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (8)
1. A simple nitrogen blow-drying device for laboratory LNP manufacture comprises a liquid bottle for placing liquid nitrogen and a fixing seat for limiting the liquid bottle; the method is characterized in that:
the fixing seat is provided with a retaining groove for limiting and preserving heat of the liquid bottle;
the liquid bottle is characterized in that a bottle stopper is detachably connected to the liquid bottle, a hose capable of being inserted into a centrifuge tube containing a sample is connected to the bottle stopper, and a water absorption layer is arranged in the hose.
2. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 1, wherein: be equipped with the spacing head that can support at the centrifuging tube orificial on the hose, the radial upward extension length of spacing head in the hose is greater than the difference of hose diameter and centrifuging tube diameter, the length that spacing head to hose inserted centrifuging tube tip is less than or equal to the length that the hose inserted the centrifuging tube.
3. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 2, wherein: the limiting head is integrally formed on the threaded cap, and the threaded cap is in threaded fit on the hose.
4. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 1, wherein: the depth of the retaining groove is two thirds of the height of the liquid containing bottle, and the thickness of the groove wall of the retaining groove is 5-10cm.
5. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 4, wherein: the outer side wall of the fixing seat is provided with a thermal insulation layer, and the thickness of the thermal insulation layer is 0.2-1cm.
6. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 5, wherein: and a limiting layer is fixedly arranged on the inner wall of the retention groove, and the limiting layer is made of fabric.
7. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 1, wherein: the flexible pipe is characterized in that an elastic bag is integrally formed on the flexible pipe, an elastic adjusting bag is integrally formed on the elastic bag, a pressing ball for shielding the flexible pipe is arranged at the joint of the elastic bag and the air outlet side of the flexible pipe, an elastic rope used for tensioning is connected between the pressing ball and the flexible pipe on the air outlet side, and a soft stay rope is connected between the pressing ball and the adjusting bag.
8. A simple nitrogen blow-dry apparatus for laboratory LNP manufacturing as in claim 7 wherein: the pressing ball is internally provided with a cavity, the wall of the cavity is mesh-shaped, and the cavity is filled with moisture-absorbing paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321196088.XU CN219977953U (en) | 2023-05-17 | 2023-05-17 | A simple and easy nitrogen gas weathers device for laboratory LNP preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321196088.XU CN219977953U (en) | 2023-05-17 | 2023-05-17 | A simple and easy nitrogen gas weathers device for laboratory LNP preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219977953U true CN219977953U (en) | 2023-11-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321196088.XU Active CN219977953U (en) | 2023-05-17 | 2023-05-17 | A simple and easy nitrogen gas weathers device for laboratory LNP preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219977953U (en) |
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2023
- 2023-05-17 CN CN202321196088.XU patent/CN219977953U/en active Active
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