CN219150967U - Tissue cleaning and preserving device for primary cell culture - Google Patents
Tissue cleaning and preserving device for primary cell culture Download PDFInfo
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- CN219150967U CN219150967U CN202320154627.7U CN202320154627U CN219150967U CN 219150967 U CN219150967 U CN 219150967U CN 202320154627 U CN202320154627 U CN 202320154627U CN 219150967 U CN219150967 U CN 219150967U
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- 238000004140 cleaning Methods 0.000 title claims abstract description 41
- 238000004113 cell culture Methods 0.000 title claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 129
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 238000005406 washing Methods 0.000 claims abstract description 33
- 239000002699 waste material Substances 0.000 claims abstract description 28
- 239000003761 preservation solution Substances 0.000 claims abstract description 27
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims description 9
- 239000012530 fluid Substances 0.000 abstract description 12
- 230000004071 biological effect Effects 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
- 230000000813 microbial effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002906 medical waste Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
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Abstract
The utility model provides a tissue cleaning and preserving device for primary cell culture, which relates to the technical field of cell culture and comprises a tissue storage test tube, a tissue preserving fluid test tube and a waste liquid storage test tube, wherein the tissue preserving fluid test tube and the waste liquid storage test tube are connected with the tissue storage test tube through pipelines; a tissue supporting net for attaching biological tissues is arranged in the tissue storage test tube; the tissue storage test tube is sealed by an embedded test tube cover and an outer test tube cover; the tissue preservation solution test tube is filled with tissue preservation solution, tissue washing liquid is arranged in the tissue preservation test tube, and the tissue washing liquid is used for submerging the tissue supporting net. The utility model carries out cleaning and preservation in the same device, so that the collection, cleaning and preservation operations of the isolated tissue sample are closed and standardized, the interference of the tissue sample acquisition process on the work of medical staff can be reduced, and the sterile state and biological activity of the isolated tissue can be fully ensured.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a tissue cleaning and preserving device for primary cell culture.
Background
The isolation and culture of primary cells from tissue samples is a widely used biological technique. After the tissue is isolated, the maintenance of the sterile state and biological activity of the tissue can fully ensure the success rate of the separation of the subsequent primary cells.
After the tissue for primary cell culture is isolated, components such as blood stain, mucus and potential pollutants on the tissue can be removed by timely and sufficient cleaning, so that the microbial pollution risk of the tissue and the interference of related impurities on subsequent experiments can be remarkably reduced. Meanwhile, providing proper storage conditions for the isolated tissues is also a key for guaranteeing the biological activity of the isolated tissues, so that the follow-up primary cell separation culture experiment can be successfully carried out. In actual operation, due to the complexity of the operation of medical personnel in the operation process, in the absence of standardized sampling, collecting, cleaning and preserving operation guidance, the isolated tissue sample can suffer from microbial contamination or biological activity damage due to the prolonged air exposure time and unsuitable storage conditions, thereby seriously affecting the subsequent primary cell culture process.
Chinese patent application number CN201822277426.8 discloses a tissue sample cleaning device, including: a cleaning container, wherein a screen is arranged in the cleaning container, a tissue sample is placed on the screen, and cleaning liquid is added in the cleaning container; the inflation device is connected with the cleaning container and is used for inputting gas into the cleaning liquid in the cleaning container; and the waste liquid recovery device is connected with the cleaning container, and waste liquid in the cleaning container is discharged into the waste liquid recovery device. When the tissue sample cleaning device is used, firstly, a tissue sample is placed on a screen in a cleaning container, then a certain amount of cleaning liquid is injected into the cleaning container, and the cleaning liquid is ensured to permeate the screen; then, gas is added into the cleaning liquid in the cleaning container through the inflation device, so that a gas-liquid stream is formed, and the tissue sample is rolled in the cleaning liquid, so that the effect of cleaning the tissue sample is achieved.
However, the device only has the function of cleaning, and can not better store the isolated tissue sample. And prior art washing and preservation are typically two procedures, requiring that the tissue sample be removed again after washing is completed and transferred to a preservation device, during which the tissue may be subject to microbial contamination or impaired biological activity due to air exposure.
Therefore, it is necessary to design a device which has good sealing performance, can perform standardized operation, and can simultaneously realize tissue cleaning and preservation.
Disclosure of Invention
In view of the above, an object of the present utility model is to provide a tissue washing and preserving device for primary cell culture.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
a tissue cleaning and preserving device for primary cell culture comprises a tissue storage tube, a tissue preserving fluid tube and a waste liquid storage tube, wherein the tissue preserving fluid tube and the waste liquid storage tube are connected with the tissue storage tube through a pipeline;
a tissue supporting net for attaching biological tissues is arranged in the tissue storage test tube;
the tissue storage test tube is sealed by an embedded test tube cover and an outer test tube cover;
the tissue preservation solution test tube is filled with tissue preservation solution, tissue washing liquid is arranged in the tissue preservation test tube, and the tissue washing liquid is used for submerging the tissue supporting net.
Preferably, the tissue storage test tube comprises an outer test tube and an embedded test tube, wherein a plurality of liquid through holes are distributed on the bottom surface of the embedded test tube, and the tissue supporting net is arranged inside the embedded test tube.
Preferably, the embedded test tube and the embedded test tube cover, the outer test tube and the outer test tube cover are all connected through screw thread sealing, the outer side surface of the embedded test tube cover is also provided with an outer screw thread, the outer test tube cover is provided with a through hole, the inner surface of the through hole is provided with an inner screw thread, and the embedded test tube cover and the outer test tube cover are connected through screw thread sealing.
Preferably, the side of the embedded test tube is provided with a vent hole.
Preferably, the distance between the tissue support net and the bottom surface of the embedded test tube is 5-10mm.
Preferably, the tissue preservation liquid test tube is communicated with the air inlet pump through a pipeline, and the waste liquid storage test tube is communicated with the air outlet pump through a pipeline.
Preferably, the pipelines are all provided with two-way connectors.
Preferably, the tissue preservation solution test tube and the waste liquid storage test tube are both provided with sealing rings.
Preferably, the upper surface of the embedded test tube cover is provided with a waterproof and breathable film.
Preferably, the mesh diameter of the tissue support net is less than or equal to 1mm.
Compared with the prior art, the utility model has the following advantages:
according to the utility model, through the arrangement of the tissue storage test tube, the tissue preservation solution test tube and the waste liquid storage test tube, the cleaning and preservation are designed in the same device, so that the problems of microbial pollution and biological activity damage caused by the prolonged exposure time of tissue air are avoided, and the collection, cleaning and preservation operation of the isolated tissue sample can be sealed and standardized. The device not only can reduce the interference of the tissue sample acquisition process to the work of medical staff, but also can fully ensure the sterile state and biological activity of the isolated tissue.
Drawings
FIG. 1 is a schematic diagram showing the structure of a tissue washing and preserving apparatus for primary cell culture;
FIG. 2 is a schematic view showing the structure of an outer tube cover in a tissue washing and preserving apparatus for primary cell culture;
FIG. 3 is a schematic view showing the structure of a built-in test tube cap in a tissue washing and preserving apparatus for primary cell culture;
FIG. 4 is a schematic view of the structure of a two-way connector in a tissue washing and preserving device for primary cell culture;
reference numerals:
1-tissue storage tube; 101-a tissue support mesh; 102-embedding a test tube; 103-outer tube;
104-an outer test tube cover; 1041-internal threads; 105-an embedded test tube cap; 1051-waterproof breathable film; 1052-external threads;
2-tissue preservation solution test tube; 201-tissue preservation solution test tube cover;
3-storing the waste liquid in a test tube; 301-storing a waste liquid test tube cover;
4-an air inlet pump; 401-an intake piston; 5-an air outlet pump; 501-an air outlet piston; 6-piping; 7-two-way connector.
Detailed Description
In order to make the purpose and technical solution of the embodiments of the present utility model more clear, the technical solution of the present utility model will be clearly and completely described below in connection with the embodiments of the present utility model.
In the description of the present application, it should be understood that terms used for indicating orientations or positional relationships are based on the orientation or positional relationships shown in the drawings, and are merely used for convenience in describing the present application and simplifying the description, and are not meant to indicate or imply that the device or element being referred to must have a particular orientation, be configured and operated in a particular orientation, and therefore should not be construed as limiting the present application.
Example 1
As shown in fig. 1 to 4, the present utility model provides a tissue washing and preserving apparatus for primary cell culture, comprising a tissue storage tube 1, a tissue preserving fluid tube 2 and a waste fluid storage tube 3, wherein the tissue storage tube 1 is used for washing and storing biological tissue samples, the tissue preserving fluid tube 2 is filled with sufficient tissue preserving fluid in advance, the tissue preserving fluid can be injected into the tissue storage tube 1 to provide good storage conditions, and the waste fluid storage tube 3 is used for receiving waste fluid generated after tissue washing. Wherein the tissue storage test tube 1, the tissue preservation solution test tube 2 and the waste liquid storage test tube 3 are communicated through a pipeline 6.
The tissue storage test tube 1 comprises an outer test tube 103 and an embedded test tube 102, wherein the outer test tube 103 and the embedded test tube 102 are coaxially arranged, the embedded test tube 102 is of a tubular structure, and a tissue supporting net 101 is embedded in the embedded test tube 102 and is used for receiving and attaching biological tissue samples; the mesh diameter of the tissue support net 101 is less than or equal to 1mm. The tissue support mesh 101 is spaced from the bottom surface of the embedded tube 102 by a distance of typically 5-10mm, preferably 10mm. The bottom surface of the embedded test tube 102 is uniformly distributed with a plurality of liquid through holes, the tissue storage test tube 1 is internally provided with tissue cleaning liquid, and the tissue cleaning liquid is used for overflowing the tissue supporting net 101. When tissue preservation liquid is introduced into the tissue storage tube 1, the tissue preservation liquid enters the embedded tube 102 through the liquid inlet; and the side of the embedded test tube 102 is provided with a vent hole, so that the air pressure in the embedded test tube 102 can be balanced in the ascending process of the tissue cleaning fluid.
The tissue preservation solution test tube 2 is filled with tissue preservation solution, the tissue preservation solution test tube 2 is communicated with the air inlet pump 4 through a pipeline 6, the air inlet pump 4 comprises an air inlet piston 401, and when the air inlet piston 401 is pressed down, the tissue preservation solution in the tissue preservation solution test tube 2 is injected into the tissue preservation solution test tube 1 as air in the air inlet pump 4 is compressed into the tissue preservation solution test tube 2; the waste liquid storage tube 3 is communicated with the air outlet pump 5 through a pipeline 6, the air outlet pump 5 comprises an air outlet piston 501, when the air outlet piston 501 is pulled up, the air pressure in the waste liquid storage tube 3 is reduced and is lower than the atmospheric pressure in the tissue storage tube 1, and at the moment, the tissue cleaning liquid of the tissue storage tube 1 is injected into the waste liquid storage tube 3. Tissue preservation solution test tube 2 seals through tissue preservation solution test tube cover 201, and waste liquid is deposited test tube 3 and is deposited test tube cover 301 through the waste liquid and seal, and all be provided with the sealing washer, guarantee the leakproofness, realized the full seal of whole device, fully guarantee the sterile condition of isolated tissue.
The pipelines 6 involved in the device are all rubber hoses, the joint of the rubber hoses and the bottle body is fixedly connected, and the pipelines 6 are connected by a double-pass connector 7. The inner diameter of the two-way connector 7 is the same as that of the pipeline 6, and the two ends of the two-way connector are expanded into a circular truncated cone shape and slightly larger than the inner diameter of the hose. After the collected tissue samples are cleaned in an operating room and stored in tissue preservation liquid, the collected tissue samples can be disconnected from front and rear pipelines through flexible pipeline design, so that the tissue samples are convenient to store.
The working principle of the tissue cleaning and preserving device for primary cell culture of the utility model is as follows:
the tissue preservation solution tube 2 is filled with the tissue preservation solution in advance, and a certain amount of tissue washing solution is injected into the tissue preservation solution tube 1 so that the liquid level is higher than the tissue supporting net 101.
The specific use method is as follows:
1. immediately unscrewing the tissue storage embedded test tube cover 105 after the medical staff draws materials, putting a tissue sample into the tissue storage embedded test tube 102, covering and screwing the tissue storage embedded test tube cover 105, attaching the tissue on the tissue supporting net 101 at the moment, and soaking the tissue in tissue cleaning liquid;
2. the medical staff pulls the air outlet piston 501 slowly, the liquid level of the tissue cleaning liquid in the tissue storage test tube 1 gradually drops due to the atmospheric pressure and slowly washes the tissue sample, and then the tissue sample gradually flows into the waste liquid storage test tube 3 until the tissue sample is emptied;
3. after the cleaning is finished, a medical staff pushes down the air inlet piston 401 to enable the tissue preservation liquid in the tissue preservation liquid test tube 2 to flow into the tissue storage outer test tube 103 and fully submerge the tissue sample in the tissue storage embedded test tube 102, so that a good storage environment is provided for the tissue sample;
4. the double-way connector 7 between the waste liquid storage test tube 3 and the tissue storage outer test tube 103 and between the tissue storage outer test tube 103 and the tissue preservation solution test tube 2 is respectively disconnected and sealed, so that the tissue storage test tube 1 is an independent and sealed part, and the rest of disconnected parts are treated by medical waste;
5. the sealed tissue storage tube 1 is properly placed in a tissue transport box and transported to a laboratory/company at 2-8 ℃ for primary cell separation.
Example two
The tissue washing and preserving apparatus for primary cell culture provided in this embodiment is similar to that in the first embodiment, but differs from the first embodiment in that:
the tissue storage tube 1 is also communicated with a tissue washing liquid tube, wherein the tissue washing liquid tube is filled with enough tissue washing liquid, and is also provided with an independent air inlet pump 4, after one washing is finished and waste liquid is discharged, the tissue washing liquid can be injected into the tissue storage tube 1 again through the air inlet pump 4, repeated washing is carried out for a plurality of times, and finally, the tissue preservation liquid in the tissue preservation liquid tube 2 is injected to preserve a tissue sample.
The foregoing is a description of embodiments of the utility model, which are specific and detailed, but are not to be construed as limiting the scope of the utility model. It should be noted that modifications and improvements, such as changing the moving structure, etc., may be made by those skilled in the art without departing from the spirit of the present utility model, and these obvious alternatives are intended to be within the scope of the present utility model.
Claims (10)
1. A tissue cleaning and preserving device for primary cell culture, characterized in that:
comprises a tissue storage test tube, a tissue preservation solution test tube and a waste liquid storage test tube, wherein the tissue preservation solution test tube and the waste liquid storage test tube are connected with the tissue storage test tube through a pipeline;
a tissue supporting net for attaching biological tissues is arranged in the tissue storage test tube;
the tissue storage test tube is sealed by an embedded test tube cover and an outer test tube cover;
the tissue preservation solution test tube is filled with tissue preservation solution, tissue washing liquid is arranged in the tissue preservation test tube, and the tissue washing liquid is used for submerging the tissue supporting net.
2. The tissue washing and preserving apparatus for primary cell culture according to claim 1, wherein: the tissue storage test tube comprises an outer test tube and an embedded test tube, a plurality of liquid through holes are distributed on the bottom surface of the embedded test tube, and the tissue support net is arranged inside the embedded test tube.
3. The tissue washing and preserving apparatus for primary cell culture according to claim 2, wherein: the embedded test tube with embedded test tube lid the outer test tube with outer test tube lid all passes through screw thread sealing connection, the lateral surface of embedded test tube lid still is equipped with the external screw thread, outer test tube lid exists a through-hole, the internal surface of through-hole is equipped with the internal thread, embedded test tube lid with pass through screw thread sealing connection between the outer test tube lid.
4. The tissue washing and preserving apparatus for primary cell culture according to claim 2, wherein: the side of embedded test tube is equipped with the air vent.
5. The tissue washing and preserving apparatus for primary cell culture according to claim 2, wherein: the distance between the tissue supporting net and the bottom surface of the embedded test tube is 5-10mm.
6. The tissue washing and preserving apparatus for primary cell culture according to claim 1, wherein: the tissue preservation liquid test tube is communicated with the air inlet pump through a pipeline, and the waste liquid storage test tube is communicated with the air outlet pump through a pipeline.
7. The tissue washing and preserving apparatus for primary cell culture according to any one of claims 1 to 6, wherein: and the pipelines are all provided with two-way connectors.
8. The tissue washing and preserving apparatus for primary cell culture according to claim 1, wherein: the tissue preservation solution test tube and the waste liquid storage test tube are both provided with sealing rings.
9. The tissue washing and preserving apparatus for primary cell culture according to claim 1, wherein: the upper surface of the embedded test tube cover is provided with a waterproof breathable film.
10. The tissue washing and preserving apparatus for primary cell culture according to claim 1, wherein: the mesh diameter of the tissue supporting net is less than or equal to 1mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320154627.7U CN219150967U (en) | 2023-01-17 | 2023-01-17 | Tissue cleaning and preserving device for primary cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320154627.7U CN219150967U (en) | 2023-01-17 | 2023-01-17 | Tissue cleaning and preserving device for primary cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219150967U true CN219150967U (en) | 2023-06-09 |
Family
ID=86638920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320154627.7U Active CN219150967U (en) | 2023-01-17 | 2023-01-17 | Tissue cleaning and preserving device for primary cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219150967U (en) |
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2023
- 2023-01-17 CN CN202320154627.7U patent/CN219150967U/en active Active
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