CN219136803U - Sample is extracted and is shifted integrative equipment of detection - Google Patents
Sample is extracted and is shifted integrative equipment of detection Download PDFInfo
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- CN219136803U CN219136803U CN202223579744.2U CN202223579744U CN219136803U CN 219136803 U CN219136803 U CN 219136803U CN 202223579744 U CN202223579744 U CN 202223579744U CN 219136803 U CN219136803 U CN 219136803U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model provides sample extraction, transfer and detection integrated equipment, which comprises a machine case, and an extraction module, a pipetting tubulation module and a detection module which are arranged in the machine case; the extraction module is used for extracting a detected object; the detection module is used for detecting the solution in the multi-connected pipe; the pipetting module comprises a pipetting mechanism and a pipetting assembly, wherein the pipetting assembly is used for driving the pipetting mechanism to move, and the pipetting mechanism is used for transferring solution to the multi-connected pipe and sealing the multi-connected pipe cover on the multi-connected pipe. The device can accomplish the extraction of sample in proper order, the transfer tubulation and the detection of reaction liquid all processes, and can pick up the rifle head simultaneously and accomplish the transfer of reaction liquid, and pick up the closing cap that the multi-tube lid accomplished the multi-tube, has effectively simplified structural arrangement, has reduced equipment occupation volume, has reached miniaturized purpose of using, has reduced sample detection cost.
Description
Technical Field
The utility model relates to the technical field of nucleic acid detection equipment, in particular to sample extraction, transfer and detection integrated equipment.
Background
Nucleic acid is a biological macromolecular compound polymerized from many nucleotides, and is one of the most basic substances for life. Nucleic acids are widely found in all animal and plant cells and microorganisms, and nucleic acids in organisms are often combined with proteins to form nucleoproteins. With the popularization of gene detection, personalized medicine administration, prenatal diagnosis and the like, the limitation of the traditional DNA extraction method is more and more obvious today in the fields of the biological industry in pursuit of high throughput and automation. The magnetic bead method for extracting nucleic acid has been gaining attention because of its capability of realizing automatic extraction, mass operation, simple operation and short time.
The magnetic bead method generally adopts a magnetic rod and magnetic bead separation technology to extract nucleic acid, usually utilizes the mutual matching motion of a magnetic rod and a magnetic rod sleeve to realize the adsorption and transfer of the magnetic beads, and simultaneously utilizes the magnetic rod sleeve to repeatedly stir and fully mix a reaction liquid system, and then the purified nucleic acid is finally obtained through the steps of sample pyrolysis, nucleic acid adsorption to the magnetic beads, magnetic bead washing, nucleic acid elution and the like.
In the existing scheme, the nucleic acid extraction and the PCR fluorescent amplification are mostly divided into two instruments, so that the operation is not convenient enough, and the occupied space of equipment is larger. Although the integrated machine integrating nucleic acid extraction and PCR fluorescence amplification is provided, the integrated machine also has the problems of huge machine body size, complex structure, high cost and the like, and can only be applied to large hospitals, but for vast middle and small hospitals, due to limited sites and insufficient funds, the integrated machine lacks suitable nucleic acid extraction, PCR fluorescence amplification integrated equipment.
In the prior art, when the nucleic acid extraction is completed, the sample tube is taken out and then put into the carrying platform of the PCR mechanism, and the deep-hole plate is not directly put into the PCR mechanism for fluorescent amplification due to the higher efficiency of extracting the nucleic acid by adopting the deep-hole plate, so that the solution in the deep-hole plate is required to be transferred into the PCR mechanism in different modes for detection.
Disclosure of Invention
The purpose of the utility model is that: aiming at the defects in the background technology, the device capable of automatically completing nucleic acid extraction and transferring to multiple tubes to put PCR fluorescent amplification is provided, and the device has a simple structure, occupies a small volume and is suitable for small and medium-sized hospitals and the like.
In order to achieve the above object, the present utility model provides a sample extraction transfer detection integrated apparatus comprising:
the device comprises a case, an extraction module, a pipetting tubing module and a detection module which are arranged in the case;
the extraction module is used for extracting a detected object;
the pipetting tubing module comprises a pipetting tubing mechanism and a pipetting movement assembly, wherein the pipetting movement assembly is used for driving the pipetting tubing mechanism to move, and the pipetting tubing mechanism is used for transferring a solution to the multi-connected tube and sealing a multi-connected tube cover on the multi-connected tube;
the detection module is used for detecting the solution in the multi-connected pipe.
Further, the extraction module is used for processing the solution in the deep hole plate, the deep hole plate is installed and positioned through a deep hole plate carrier, and samples or processing liquid are filled in all deep holes of the deep hole plate;
the extraction module comprises a magnetic rod sleeve assembly and a magnetic rod assembly;
the magnetic rod sleeve assembly comprises a first mounting frame connected with a first driving assembly and a magnetic rod sleeve arranged on the first mounting frame, and the first driving assembly is used for driving the first mounting frame to move along the Z direction;
the magnetic rod assembly comprises a second mounting frame connected with a second driving assembly and a magnetic rod arranged on the second mounting frame, and the second driving assembly is used for driving the second mounting frame to move along the Z direction;
a first guide rail is arranged in the case, a first sliding block is arranged on the deep hole plate carrier, and the first sliding block is in sliding connection with the first guide rail; the deep hole plate carrier is provided with a first connecting block with a threaded hole, the first connecting block is in threaded fit with the first screw rod, and the first motor is in transmission connection with the first screw rod.
Further, the pipetting tubing module comprises a gun head material frame, a multi-tube cover charging plate and a multi-tube bearing platform, wherein the gun head material frame is used for charging the gun head, the multi-tube cover charging plate is used for charging the multi-tube cover, and the multi-tube bearing platform is used for charging the multi-tube.
Further, the multi-tube cover loading plate is provided with a plurality of cover loading holes of the multi-tube cover, and the multi-tube bearing platform is provided with a plurality of multi-tube loading holes.
Further, the pipetting tubulation mechanism comprises a connecting part and pipetting tubulation parts, each connecting part of the pipetting tubulation mechanism is connected with the pipetting tubulation parts and sequentially arranged, and a runner is arranged in the pipetting tubulation part corresponding to one connecting part and used for generating negative pressure and positive pressure.
Further, the pipetting motion assembly comprises a pipetting Z-direction assembly, a pipetting X-direction assembly and a pipetting Y-direction assembly, wherein the pipetting Z-direction assembly is used for driving the pipetting tubing mechanism to move along the Z direction, the pipetting X-direction assembly is used for driving the pipetting tubing mechanism to move along the X direction, and the pipetting Y-direction assembly is used for driving the pipetting tubing mechanism to move along the Y direction.
Further, the pipetting X-direction assembly comprises an X-direction mounting frame, a first synchronous belt mechanism and a second guide rail are arranged on the X-direction mounting frame, the pipetting Z-direction assembly is in sliding fit with the second guide rail through a second sliding block, and the pipetting Z-direction assembly is connected with the first synchronous belt mechanism through a second connecting block;
the pipetting Z-direction assembly comprises a mounting seat connected with a second sliding block and a second connecting block, the mounting seat is provided with a third guide rail and a second screw rod, the second screw rod is connected with a second motor, the pipetting tubing mechanism is in sliding fit with the third guide rail through the third sliding block, the pipetting tubing mechanism is provided with a third connecting block, and the third connecting block is provided with a threaded hole and is in threaded fit with the second screw rod;
the pipetting Y-direction assembly comprises a Y-direction installation frame, a second synchronous belt mechanism and a fourth guide rail are arranged on the Y-direction installation frame, the X-direction installation frame is in sliding fit with the fourth guide rail through a fourth sliding block, and the X-direction installation frame is connected with the second synchronous belt mechanism through a fourth connecting block.
Further, a first assembly hole is formed in the top end of the gun head, a second assembly hole is formed in the top end of the multi-joint pipe cover, and the connecting portion can be inserted into the first assembly hole or the second assembly hole to form interference fit.
Further, the pipetting module is further provided with a release mechanism connected with the pipetting tube and movable relative to the pipetting tube for releasing the gun head and the multi-tube cap from the connection.
Further, the detection module comprises a PCR amplification module, and the PCR amplification module performs amplification detection on the solution in the multi-connected tube.
The scheme of the utility model has the following beneficial effects:
the sample extraction, transfer and detection integrated equipment provided by the utility model can sequentially complete the extraction of samples, transfer tubing of reaction liquid and detection of all working procedures, and the transfer and execution assembly can simultaneously pick up the gun head to complete the transfer of the reaction liquid and pick up the multi-tube cover to complete the sealing cover of the multi-tube, and simultaneously adapt to the structures of the gun head and the multi-tube cover, so that all working procedures of the transfer tubing and the sealing cover can be completed only by arranging a single transfer tubing mechanism, the structural arrangement is effectively simplified, the occupied volume of the equipment is reduced, the purpose of miniaturized use is achieved, the sample detection cost is reduced, and the equipment is suitable for the conditions of limited sites, insufficient funds and the like of vast small and medium hospitals and has obvious market competitiveness;
in the utility model, each driving component in the case relates to the movement in the Z direction, the Y direction and the X direction, the collection degree of the whole equipment is higher, and the setting of the control steps is simpler;
other advantageous effects of the present utility model will be described in detail in the detailed description section which follows.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic diagram of a nucleic acid extraction module according to the present utility model;
FIG. 3 is a schematic diagram of a deep well plate and deep well plate drive assembly according to the present utility model;
FIG. 4 is a schematic view of a gun head material rack, a multi-tube cover loading plate and a multi-tube carrier of the present utility model;
FIG. 5 is a schematic diagram of a mobile execution assembly according to the present utility model;
FIG. 6 is a schematic view of a pipetting X-direction assembly and a pipetting Y-direction assembly according to the utility model;
FIG. 7 is a schematic view of a pipetting Z-directed assembly of the utility model;
FIG. 8 is a schematic view of a multi-gang lid loading plate of the present utility model;
FIG. 9 is a schematic view of a multi-pipe carrier of the present utility model.
[ reference numerals description ]
100-a case; 200-a nucleic acid extraction module; 210-a magnetic rod sleeve assembly; 211-a first drive assembly; 212-a first mount; 213-magnetic bar sleeve; 220-magnetic bar assembly; 221-a second drive assembly; 222-a second mount; 223-magnetic bar; 230-a deep-well plate drive assembly; 231-a first rail; 232-a first slider; 233-a first motor; 234-a first screw; 235-a first connection block; 240-deep well plate carriers; 241-deep well plate; 300-pipetting tubing module; 310-gun head material rack; 311-gun head; 3111-first mounting hole; 320-a multi-tube cap loading plate; 321-a multi-connected pipe cover; 3211-a second fitting hole; 322-cover hole; 330-a multi-connected pipe bearing platform; 331-a multi-connected pipe; 332-filling holes; 340-a mobile execution component; 341-pipetting a Z-direction assembly; 3411-mount; 3412-third guide rail; 3413-a second screw; 3414-a second motor; 3415-third slider; 3416-third connection block; 342-pipetting tubing mechanism; 3421-pipetting tubing; 3422-a connection; 343-pipetting an X-direction assembly; 3431-X direction mounting frame; 3432-a first timing belt mechanism; 3433-a second rail; 3434-a second slider; 3435-a second connection block; a 400-PCR amplification module; 344-pipetting Y-direction assembly; 3441-Y direction mounting rack; 3442-a second timing belt mechanism; 3443-fourth rail; 3444-fourth slider; 350-a waste gun head collecting module.
Detailed Description
In order to make the technical problems, technical solutions and advantages to be solved more apparent, the following detailed description will be given with reference to the accompanying drawings and specific embodiments. It will be apparent that the described embodiments are some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model. In addition, the technical features of the different embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, a locked connection, a removable connection, or an integral connection; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1 to 4, an embodiment of the present utility model provides a sample extraction transfer detection integrated apparatus, which includes a casing 100 and a plurality of functional modules disposed in the casing 100, and the functional modules include a nucleic acid extraction module 200, a pipetting module 300 and a PCR amplification module 400. The nucleic acid extraction module 200 performs nucleic acid extraction by using a magnetic bead method, the pipetting tubulation module 300 transfers the reaction solution containing nucleic acid in the deep hole plate 241 into the multi-connected tube 331 and seals the multi-connected tube 331, and transfers the sealed multi-connected tube 331 to the detection module, wherein the detection module 400 comprises a PCR amplification instrument, and the PCR amplification instrument performs amplification detection on the reaction solution in the multi-connected tube 331.
In this embodiment, the deep well plate 241 has a plurality of columns of deep wells, each column of deep wells containing a different liquid, such as a nucleic acid extraction solution, a wash solution, an eluent, etc., while each column of deep wells includes an equal number of, typically eight, wells for performing a nucleic acid extraction in groups of eight at a time. Nucleic acid extraction module 200 includes a magnetic rod sheath assembly 210 and a magnetic rod assembly 220. The deep hole plate 241 is mounted through the deep hole plate carrier 240, and the deep hole plate carrier 240 is provided with a slot for placing and positioning the deep hole plate 241, and each deep hole of the deep hole plate 241 is filled with a sample, a washing liquid, an eluent and the like of nucleic acid to be extracted. The magnetic rod sleeve assembly 210 and the magnetic rod assembly 220 extract nucleic acid from the sample in the deep hole plate 241 by a magnetic bead method, and when the nucleic acid extraction is completed on the sample, the deep hole plate 241 needs to be replaced, so that the deep hole plate 241 newly filled with the sample and the treatment fluid is replaced on the deep hole plate carrier 240 to prepare for the next round of extraction.
Referring again to fig. 2, the magnetic rod sleeve assembly 210 includes a first mounting frame 212 connected to a first driving assembly 211, and a magnetic rod sleeve 213 mounted on the first mounting frame 212, wherein the first driving assembly 211 is used for driving the first mounting frame 212 to move along the Z direction. The magnetic bar assembly 220 includes a second mounting frame 222 coupled to the second driving assembly 221, and a magnetic bar 223 mounted on the second mounting frame 222. The second driving component 221 is configured to drive the second mounting frame 222 to move along the Z direction, so as to drive the magnetic rod 223 on the second mounting frame 222 to move along the Z direction.
The first driving component 211 is used for driving the first mounting frame 212 and the magnetic rod sleeve 213 on the first mounting frame 212 to move along the Z direction (up and down), so that the magnetic rod sleeve is inserted into the deep hole plate 241 to extract a sample, and meanwhile, the sample is uniformly mixed in an oscillating mode. After the uniform mixing is completed, the second driving assembly 221 drives the second mounting frame 222 and the magnetic rods 223 to be inserted into the magnetic rod sleeve 213 in a one-to-one correspondence manner, so that the magnetic beads are adsorbed on the surface of the magnetic rod sleeve 213. And sequentially inserting the magnetic rod sleeve 213 adsorbed with the magnetic beads into the deep hole filled with the washing liquid and the deep hole filled with the eluting liquid, then washing and eluting, and finally retaining the nucleic acid eluted by the magnetic beads in the eluting liquid for amplification detection.
Therefore, the magnet bar sleeve assembly 210 and the magnet bar assembly 220 are required to be moved and switched to be above different deep holes, namely, the deep hole plate carrier 240 is also required to be controlled to translate relative to the magnet bar sleeve assembly 210 and the magnet bar assembly 220. Specifically, in this embodiment, the deep hole plate carrier 240 is connected to the deep hole plate driving assembly 230, and the deep hole plate driving assembly 230 is used to drive the deep hole plate carrier 240 to move along the X direction, so that the deep hole plate carrier 240 completes the adjustment of the X direction position. In addition, the deep well plate carrier 240 can also be moved close to the pipetting module 300 to facilitate transfer of the reaction fluids.
Wherein the deep-hole plate driving assembly 230 adopts a screw driving and guide rail guiding mode. Referring to fig. 3 again, in the embodiment, a first guide rail 231 is disposed in the chassis 100, a first slider 232 is disposed at the bottom end of the deep hole board carrier 240, and the first slider 232 is slidably connected with the first guide rail 231. Meanwhile, a first motor 233 and a first screw rod 234 are arranged in the case 100, a first connecting block 235 with a threaded hole is arranged in the deep hole plate carrier 240, the first connecting block 235 is in threaded fit with the first screw rod 234 through the threaded hole, the first motor 233 drives the first screw rod 234, and the position of the deep hole plate carrier 240 is adjusted, so that the purpose of switching the positions of deep holes is achieved.
It should be noted that, in this embodiment, in order to further improve the efficiency, the deep holes on the deep hole plate 241 are at least divided into two groups, and each group of deep holes is sequentially filled with a nucleic acid extracting solution, a washing solution, an eluent, etc., so that the nucleic acid extracting module 200 can simultaneously extract multiple rows of nucleic acids, thereby effectively improving the working efficiency.
Referring to fig. 4 and 5, in the present embodiment, the pipette module 300 includes a gun head rack 310, a multi-tube cap loading plate 320, a multi-tube stage 330, and a pipette tip assembly 340. Wherein, the gun head material frame 310, the multi-tube cover charging plate 320 and the multi-tube bearing platform 330 are sequentially arranged, the gun head 311 is arranged in the gun head material frame 310, and the gun head 311 is of a hollow tubular structure and is used for transferring reaction liquid.
It can be understood that the gun heads 311 are consumable materials, and each gun head 311 is disabled after the transfer of the reaction liquid is completed, so as to avoid the influence on the detection accuracy caused by the pollution of the next batch of reaction liquid during the next transfer. Therefore, the gun head 311 is detachably disposed on the moving and assembling component 340, and the moving and assembling component 340 takes a new gun head 311 from the gun head rack 310, moves to above the deep hole plate 241, sucks the reaction liquid, and moves to the multi-connected tube support 330 to transfer the reaction liquid into the multi-connected tubes 331 in a one-to-one correspondence. The gun head 311 is moved above the waste gun head collecting module 350 by the moving and loading executing assembly 340 after completing the transfer of the reaction liquid, and the waste gun head 311 is discharged and placed in the waste gun head collecting module 350.
In this embodiment, the pipetting performing assembly 340 comprises a pipetting Z-direction assembly 341 and a pipetting tubing mechanism 342. The pipetting Z-direction assembly 341 can drive the pipetting tubulation mechanism 342 to reciprocate along the Z-direction, so that the gun head 311 assembled on the pipetting tubulation mechanism 342 reciprocates along the Z-direction, the gun head 311 is convenient to extend into the deep hole plate 241 to suck or discharge reaction liquid into the multi-joint tube 331, and the gun head 311 is convenient to assemble and disassemble.
Meanwhile, the pipette mechanism 342 can generate negative pressure for sucking the solution in the deep well plate 241 by the gun head 311 or positive pressure for discharging the solution from the gun head 311 to the manifold 331. Therefore, when transferring the solution, the pipette tip 311 and the pipette tip 342 move downward in the Z direction, and the pipette tip 311 is inserted into the corresponding deep hole of the deep hole plate 241 (filled with the reaction solution), and the pipette tip 311 generates negative pressure, so that the reaction solution is sucked into the pipette tip 311. Then, the pipette and tubing mechanism 342 drives the gun head 311 to move upwards along the Z direction, so that the gun head 311 moves out of the deep hole plate 241 and moves transversely integrally, the pipette and tubing mechanism 342 and the gun head 311 synchronously move above the multi-connected tube 331 along the X direction and move downwards along the Z direction, so that the gun head 311 is inserted into the multi-connected tube 331, the pipette and tubing mechanism 342 generates positive pressure, the reaction liquid in the gun head 311 is discharged into the multi-connected tube 331, and the transfer of the reaction liquid is completed.
In this embodiment, the pipetting mechanism 342 includes a pipetting tube portion 3421 and a plurality of connection portions 3422, wherein the plurality of connection portions 3422 are connected with the pipetting tube portion 3421 and are sequentially arranged in the Y direction, and a plurality of flow channels are disposed in the pipetting tube portion 3421 and can be in one-to-one correspondence with the plurality of connection portions 3422, and one connection portion 3422 is used for being connected with one gun head 311. Therefore, the pipette mechanism 342 can transfer the reaction liquid in a plurality of deep holes at the same time, and the number of holes in each row of the deep hole plate 241 is also one-to-one. Of course, as a further improvement, two or more deep well plates 241 may be provided in the Y direction, and at this time, the number of the connecting portions 3422 is arranged in the Y direction at the same time in a corresponding number, enabling nucleic acid extraction to be performed on more deep well plates 241 at the same time.
As can be seen from the foregoing steps, the pipette tip mechanism 342 and the gun tip 311 are required to move in the X-direction, and therefore, the pipette tip X-direction assembly 343 is required to be provided, and the pipette tip 311 is required to move in the Y-direction, and therefore, the pipette tip Y-direction assembly 344 is required to be provided. Pipetting Y-direction assembly 344 is disposed in the Y-direction, pipetting X-direction assembly 343 is disposed in the X-direction and mounted on pipetting Y-direction assembly 344, and pipetting Z-direction assembly 341 is mounted on pipetting X-direction assembly 343.
Meanwhile, as shown in fig. 6, in this embodiment, the pipetting X-direction assembly 343 includes an X-direction mounting frame 3431, a first synchronous belt mechanism 3432 and a second guide rail 3433 are disposed on the X-direction mounting frame 3431, the pipetting Z-direction assembly 341 is slidably matched with the second guide rail 3433 through a second slide block 3434 connected with the pipetting X-direction assembly 341 and is connected with the first synchronous belt mechanism 3432 through a second connecting block 3434 connected with the pipetting Z-direction assembly 341, so that the pipetting Z-direction assembly 341 is guided and driven along the X-direction by the pipetting X-direction assembly 343.
The pipetting Y-direction assembly 344 comprises a Y-direction mounting frame 3441, a second synchronous belt mechanism 3442 and a fourth guide rail 3443 are arranged on the Y-direction mounting frame 3441, the X-direction mounting frame 3431 is in sliding fit with the fourth guide rail 3443 through a fourth sliding block 3444 connected with the Y-direction mounting frame 3431, and the X-direction mounting frame 3431 is connected with the second synchronous belt mechanism 3442 through a fourth connecting block connected with the X-direction mounting frame, so that the pipetting X-direction assembly 343 is guided and driven in the Y direction through the pipetting Y-direction assembly 344.
Meanwhile, as shown in fig. 7, the pipetting Z-direction assembly 341 includes a mounting seat 3411, where the mounting seat 3411 is disposed along the Z-direction and a third guide rail 3412 is disposed along the Z-direction in the present embodiment. Meanwhile, the mounting seat 3411 is further provided with a second screw rod 3413 along the Z direction, and the second screw rod 3413 is connected with the second motor 3414 to be driven to rotate by the second motor 3414. Correspondingly, the pipetting mechanism 342 is slidably matched with the third guide rail 3412 through the third slider 3415 connected with the pipetting mechanism 342, meanwhile, the pipetting mechanism 342 is also connected with the third connecting block 3416, the third connecting block 3416 is provided with a threaded hole and is in threaded fit with the second screw rod 3413, and therefore the second motor 3414 is used for controlling the position and movement of the pipetting mechanism 342 in the Z direction, and a plurality of actions are completed.
It will be appreciated that in other embodiments, the pipetting X-direction assembly 343, the pipetting Y-direction assembly 344 and the pipetting Z-direction assembly 341 can be driven in other ways, and are not particularly limited herein.
Referring to fig. 4 again, in the present embodiment, the connecting portion 3422 is engaged with the gun head 311 in a clamping manner, that is, the top end of the gun head 311 is provided with a first assembly hole 3111, the connecting portion 3422 has a columnar structure, and can be inserted into the first assembly hole 3111 of the gun head 311 to form an interference fit, so that the gun head 311 is stably connected with the connecting portion 3422. The disassembly of the gun head 311 is completed by external force. Thus, in this embodiment, the pipette module 300 further comprises a release mechanism connected to the pipette tubing section 3421 and movable relative to the pipette tubing section 3421 to apply an outward force to the gun head 311 or the like relative to the connection section 3422 to release the gun head 311 from the connection section 3422.
Referring to fig. 8, in the present embodiment, the multi-tube cap loading plate 320 is provided with a plurality of cap holes 322 of the multi-tube cap 321, which are in one-to-one correspondence with the number of connecting portions 3422 of the multi-tube 331 and the pipetting tube mechanism 342. Therefore, after the transfer of the reaction solution to the multi-manifold 331 is completed by the pipetting mechanism 342, the multi-manifold cap 321 is fetched above the multi-manifold cap loading plate 320, and finally the multi-manifold cap 321 is capped on the multi-manifold 331. The multiplex tube 331 can then be moved directly to a PCR amplification apparatus for amplification.
It will be appreciated that the pipette mechanism 342 is required to discard the assembled gun head 311 after completion of the transfer of the reaction solution and pick up the multi-tube cap 321 via the connection 3422. Therefore, in this embodiment, the top end of the multi-tube cover 321 is provided with the second assembly hole 3211, and the connecting portion 3422 can also be inserted into the second assembly hole 3211 on the multi-tube cover 321 to form an interference fit, so that the multi-tube cover 321 and the connecting portion 3422 are stably connected. When the multi-tube cap 321 is closed to the multi-tube 331, the releasing mechanism can also release the multi-tube cap 321 from the connecting portion 3422, thereby completing the cap-mounting operation of the multi-tube 331.
Referring to fig. 9, in the present embodiment, the multiple tube support 330 is provided with a plurality of tube holes 332 of the multiple tube 331, and the multiple tubes 331 are arranged in eight rows and are connected to each other, so that the multiple tube support 330 is also provided with eight rows of tube holes 332, so that the multiple tubes 331 can be stably placed in the tube holes 332. It should be noted that, each tube in the multiple tubes 331 is connected as a whole through a connection plate, and the depth of the tube holes 332 in this embodiment is matched with the depth of the multiple tubes 331 below the connection plate, so that after the multiple tubes 331 are positioned, the connection plate can be supported by the upper surface of the multiple tube bearing platform 330 to keep stable. When the multiplex 331 is filled with the reaction solution and covered, the whole multiplex 331 can be moved to the PCR amplification module 400 for amplification.
In general, with the sample extraction, transfer and detection integrated device provided in this embodiment, after the deep hole plate 241, the gun head 311, the multi-connected tube cover 321 and the multi-connected tube 331 are fed, the nucleic acid extraction assembly 200 sequentially completes magnetic bead extraction, washing and elution through the magnetic rod sleeve assembly 210 and the magnetic rod assembly 220 to obtain a nucleic acid reaction solution, and then transfers the reaction solution through the transfer execution assembly 340. After the pipette tip 311 is picked up from the gun tip material frame 310 and assembled, the gun tip 311 is driven to be inserted into a deep hole filled with the reaction liquid, the reaction liquid is adsorbed into the gun tip 311 under the action of negative pressure, and then the reaction liquid is moved to the multi-connecting tube 331 to generate positive pressure so that the reaction liquid is discharged to the multi-connecting tube 331. Then, the pipette tip 311 is removed by the pipette tip mechanism 342, and the pipette tip mechanism is moved to the position of the multi-tube cover 321 to pick up the multi-tube cover 321, and the multi-tube cover 321 is covered on the multi-tube 331, so that the filling of a group of multi-tube 331 is completed, and the next cycle is performed.
By adopting the sample extraction, transfer and detection integrated equipment provided by the embodiment, all procedures of nucleic acid extraction, transfer tubing of reaction liquid and PCR amplification can be sequentially completed, and the transfer and execution assembly 340 can pick up the gun head 311 to complete the transfer of the reaction liquid and pick up the multi-connected tube cover 321 to complete the sealing cover of the multi-connected tube 331, so that the structure of the gun head 311 and the multi-connected tube cover 321 is adapted. Therefore, all procedures of pipetting tubulation and sealing can be completed by only arranging a single pipetting tubulation mechanism 342, so that the structural arrangement is effectively simplified, the occupied volume of equipment is reduced, the purpose of miniaturized use is achieved, the nucleic acid detection cost is reduced, the pipetting tubulation and sealing device is suitable for situations of limited sites, insufficient funds and the like of vast middle and small hospitals, and has obvious market competitiveness.
While the foregoing is directed to the preferred embodiments of the present utility model, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present utility model, and such modifications and adaptations are intended to be comprehended within the scope of the present utility model.
Claims (10)
1. Sample draws and shifts integrative equipment of detection, its characterized in that: the sample extraction transfer detection integrated device comprises:
the device comprises a case, an extraction module, a pipetting tubing module and a detection module which are arranged in the case;
the extraction module is used for extracting a detected object;
the pipetting tubing module comprises a pipetting tubing mechanism and a pipetting movement assembly, wherein the pipetting movement assembly is used for driving the pipetting tubing mechanism to move, the pipetting tubing mechanism is used for transferring a solution to multiple tubes, and a multiple tube cover is covered on the multiple tubes;
the detection module is used for detecting the solution in the multi-connected pipe.
2. The integrated sample extraction, transfer and detection device according to claim 1, wherein the extraction module processes the solution in the deep well plate, the deep well plate is installed and positioned by a deep well plate carrier, and the sample or the processing solution is filled in each deep hole of the deep well plate;
the extraction module comprises a magnetic rod sleeve assembly and a magnetic rod assembly;
the magnetic rod sleeve assembly comprises a first mounting frame connected with a first driving assembly and a magnetic rod sleeve arranged on the first mounting frame, and the first driving assembly is used for driving the first mounting frame to move along the Z direction;
the magnetic rod assembly comprises a second mounting frame connected with a second driving assembly and a magnetic rod arranged on the second mounting frame, and the second driving assembly is used for driving the second mounting frame to move along the Z direction;
a first guide rail is arranged in the case, a first sliding block is arranged on the deep hole plate carrier, and the first sliding block is in sliding connection with the first guide rail; the deep hole plate carrier is provided with a first connecting block with a threaded hole, the first connecting block is in threaded fit with the first screw rod, and the first motor is in transmission connection with the first screw rod.
3. The integrated sample extraction and transfer testing device of claim 1, wherein the pipetting module comprises a gun head rack for loading gun heads, a multi-tube lid loading plate for loading the multi-tube lids, and a multi-tube stage for loading the multi-tubes.
4. The integrated sample extraction, transfer and detection device according to claim 3, wherein the multi-tube cover loading plate is provided with a plurality of cover loading holes of the multi-tube cover, and the multi-tube bearing platform is provided with a plurality of pipe loading holes of the multi-tube.
5. A sample extraction transfer test integrated apparatus according to claim 3, wherein the pipetting tubulation mechanism comprises a connection portion and pipetting tubulation portions, each connection portion of the pipetting tubulation mechanism is connected to the pipetting tubulation portions and arranged in sequence, and a flow channel is provided in the pipetting tubulation portion corresponding to one connection portion for generating negative pressure and positive pressure.
6. The integrated sample extraction transfer assay device of claim 1, wherein the pipetting motion assembly comprises a pipetting Z-direction assembly for driving the pipetting tubing mechanism to move in the Z-direction, a pipetting X-direction assembly for driving the pipetting tubing mechanism to move in the X-direction, and a pipetting Y-direction assembly for driving the pipetting tubing mechanism to move in the Y-direction.
7. The integrated sample extraction, transfer and detection device according to claim 6, wherein the pipetting X-direction assembly comprises an X-direction mounting frame, a first synchronous belt mechanism and a second guide rail are arranged on the X-direction mounting frame, the pipetting Z-direction assembly is in sliding fit with the second guide rail through a second sliding block, and the pipetting Z-direction assembly is connected with the first synchronous belt mechanism through a second connecting block;
the pipetting Z-direction assembly comprises a mounting seat connected with a second sliding block and a second connecting block, the mounting seat is provided with a third guide rail and a second screw rod, the second screw rod is connected with a second motor, the pipetting tubing mechanism is in sliding fit with the third guide rail through the third sliding block, the pipetting tubing mechanism is provided with a third connecting block, and the third connecting block is provided with a threaded hole and is in threaded fit with the second screw rod;
the pipetting Y-direction assembly comprises a Y-direction installation frame, a second synchronous belt mechanism and a fourth guide rail are arranged on the Y-direction installation frame, the X-direction installation frame is in sliding fit with the fourth guide rail through a fourth sliding block, and the X-direction installation frame is connected with the second synchronous belt mechanism through a fourth connecting block.
8. The integrated sample extraction, transfer and detection device of claim 5, wherein a first assembly hole is formed in the top end of the gun head, a second assembly hole is formed in the top end of the multi-tube cap, and the connecting portion can be inserted into the first assembly hole or the second assembly hole to form interference fit.
9. The integrated sample extraction transfer test apparatus of claim 8, wherein the pipetting module is further provided with a release mechanism coupled to the pipetting tubing and movable relative thereto to release the gun head and the multi-tube cap from the connection.
10. The integrated sample extraction transfer assay device of claim 1, wherein the assay module comprises a PCR amplification module that performs amplification assays on solutions within a multiplex tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223579744.2U CN219136803U (en) | 2022-12-31 | 2022-12-31 | Sample is extracted and is shifted integrative equipment of detection |
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| CN202223579744.2U CN219136803U (en) | 2022-12-31 | 2022-12-31 | Sample is extracted and is shifted integrative equipment of detection |
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| CN219136803U true CN219136803U (en) | 2023-06-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118909751A (en) * | 2024-07-22 | 2024-11-08 | 四川省轻工业研究设计院有限公司 | Treatment system for detecting microbial solution |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118909751A (en) * | 2024-07-22 | 2024-11-08 | 四川省轻工业研究设计院有限公司 | Treatment system for detecting microbial solution |
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