CN219065487U - Flow channel chip and detection device with same - Google Patents

Abstract

The utility model provides a runner chip and a detection device with the same, wherein the runner chip comprises a glass slide, a cover glass and a bonding layer, the bonding layer is provided with a through runner contour opening, the inner side wall of the runner contour opening, the upper surface of the glass slide and the lower surface of the cover glass enclose a runner cavity, and the runner cavity comprises a liquid inlet channel, a dyeing cavity and a liquid outlet channel in the length direction of the glass slide; the slide glass or the cover glass is provided with a liquid inlet communicated with the inlet of the liquid inlet channel in a penetrating way, a plurality of outlets of the liquid inlet channel are distributed at intervals along the width direction of the slide glass and are communicated with the inlet of the dyeing cavity, a plurality of inlets of the liquid outlet channel are distributed at intervals along the width direction of the slide glass and are communicated with the outlet of the dyeing cavity, and a liquid outlet communicated with the outlet of the liquid outlet channel is arranged on the slide glass or the cover glass in a penetrating way. Through the technical scheme that this application provided, can solve the extravagant and automatic problem of fluorescent staining reagent of runner chip among the correlation technique.

Description

Flow channel chip and detection device with same

Technical Field

The utility model relates to the technical field of microfluidics, in particular to a flow channel chip and a detection device with the same.

Background

Fluorescence microscopy is one of the key technologies in the life sciences field, and proteomics methods combine fluorescence microscopy with complex protocols, and can visually observe protein components of a sample in millions of pixels to obtain various protein compositions of an organism and relations among the protein compositions. Robust, repeatable data generation of proteomics methods is achieved by automatically performing and precisely controlling temperature, reagent application, and image acquisition parameters over days or weeks of iterative chemistry and imaging cycles.

In the related art, the runner chip includes slide glass, coverslip and erect the tie coat between slide glass and coverslip, and the tie coat has the runner profile opening of running through the design, places the sample on the slide glass, and runner chamber is enclosed jointly to runner profile open-ended inside wall, slide glass's upper surface and coverslip's lower surface, and the sample is located runner intracavity, runs through on the coverslip to be provided with the inlet and the liquid outlet that are linked together with the runner chamber for the runner chamber is the confined fluid chamber that has input and output port, uses immunohistochemical staining etc. technique processing sample on the slide glass.

However, the size of the flow channel cavity determines the amount of the fluorescent staining reagent used, and since the flow channel cavity of the flow channel chip in the related art has a simple square structure, the surface area of the sample is much smaller than that of the flow channel cavity, so that the flow channel chip in the related art has a problem of wasting the fluorescent staining reagent.

Disclosure of Invention

The utility model provides a flow channel chip and a detection device with the same, which are used for solving the problem of waste of fluorescent staining reagent of the flow channel chip in the related technology.

According to an aspect of the present utility model, there is provided a flow channel chip including: a glass slide; a cover glass, which is covered on the glass slide; the adhesive layer is arranged between the glass slide and the cover glass, the adhesive layer is provided with a runner contour opening which is arranged in a penetrating manner, the inner side wall of the runner contour opening, the upper surface of the glass slide and the lower surface of the cover glass jointly enclose a runner cavity, and the runner cavity comprises a liquid inlet channel, a dyeing cavity and a liquid outlet channel which are communicated in sequence in the length direction of the glass slide; the slide glass or the cover glass is provided with a liquid inlet communicated with the inlet of the liquid inlet channel in a penetrating manner, the liquid inlet channel comprises a plurality of outlets which are arranged at intervals along the width direction of the slide glass, the outlets of the liquid inlet channel are communicated with the inlet of the dyeing cavity, the liquid outlet channel comprises a plurality of inlets which are arranged at intervals along the width direction of the slide glass, the inlets of the liquid outlet channel are communicated with the outlet of the dyeing cavity, and the slide glass or the cover glass is provided with a liquid outlet communicated with the outlet of the liquid outlet channel in a penetrating manner.

Further, the liquid inlet channel and the liquid outlet channel are both in a tree structure.

Further, along the length direction of the glass slide, the liquid inlet channel comprises N liquid inlet channel layers which are sequentially communicated; wherein N is a positive integer greater than or equal to 2, and the Nth liquid inlet flow channel layer is provided with 2 ^N-1 The Nth inlet flow channel layer is provided with 2 ^N And a plurality of outlets.

Further, along the length direction of the glass slide, the liquid outlet channel comprises N liquid outlet channel layers which are sequentially communicated; wherein N is a positive integer greater than or equal to 2, and the Nth liquid outlet channel layer is provided with 2 ^N-1 The Nth liquid outlet channel layer has 2 ^N And a plurality of inlets.

Further, the pipe diameters of the branch flow passages of the same level of the tree structure are the same.

Further, the flow channel cavity is of a central symmetry structure.

Further, the runner cavity further comprises a liquid inlet cavity and a liquid outlet cavity, the liquid inlet is communicated with the inlet of the liquid inlet channel through the liquid inlet cavity, and the liquid outlet is communicated with the outlet of the liquid outlet channel through the liquid outlet cavity.

Further, a plurality of liquid inlets are arranged on the glass slide at intervals along the width direction of the glass slide, and a plurality of liquid outlets are arranged on the glass slide at intervals along the width direction of the glass slide; and/or the liquid inlet cavity and the liquid outlet cavity are bar-shaped structures extending along the width direction of the glass slide.

Further, the runner chip further comprises a positioning plate, a positioning opening is formed in the positioning plate, and the glass slide, the cover glass and the bonding layer are embedded in the positioning opening; the locating plate is provided with a locating hole, and the locating hole is positioned at the edge of the locating opening.

According to another aspect of the present utility model, there is provided a detection device including a fluid storage member, a flow channel chip, a negative pressure member, and a waste liquid recovery member, wherein a liquid inlet of the flow channel chip is connected to an outlet of the fluid storage member, the negative pressure member is connected to a liquid outlet of the flow channel chip, an inlet of the waste liquid recovery member is connected to a liquid outlet of the flow channel chip, and the flow channel chip is provided as described above.

By applying the technical scheme of the utility model, the flow channel chip comprises a glass slide, a cover glass and a bonding layer, and as the inner side wall of the flow channel outline opening, the upper surface of the glass slide and the lower surface of the cover glass enclose a flow channel cavity together, the flow channel cavity comprises a liquid inlet channel, a dyeing cavity and a liquid outlet channel which are sequentially communicated in the length direction of the glass slide, a sample is placed on the glass slide, the sample is exposed in the dyeing cavity, a fluorescent dyeing reagent is added to the inlet of the liquid inlet channel through the liquid inlet of the glass slide or the cover glass, the fluorescent dyeing reagent sequentially passes through the liquid inlet channel, the dyeing cavity and the liquid outlet channel, and then the fluorescent dyeing operation on the section of the sample in the dyeing cavity is completed, and the sample is discharged from the flow channel cavity through the liquid outlet on the glass slide or the cover glass. Therefore, through designing runner profile open-ended shape, on the length direction of slide glass for the runner chamber is including feed liquor passageway, dyeing chamber and the drain channel of intercommunication in proper order, exposes the sample in the dyeing intracavity, under the prerequisite of guaranteeing sample and fluorescent dye fully contact, utilizes feed liquor passageway and the drain channel of both sides, reduces the area in runner chamber, thereby reduces fluorescent dye reagent's use amount, practices thrift reagent use cost.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model. In the drawings:

fig. 1 shows an exploded view of a flow channel chip according to a first embodiment of the present utility model;

fig. 2 is a schematic structural diagram of an adhesive layer of a runner chip and a runner cavity according to a first embodiment of the present utility model;

fig. 3 is a schematic structural diagram of a positioning plate of a flow channel chip according to a first embodiment of the present utility model;

FIG. 4 is a schematic view showing a process of placing a sample of a flow channel chip onto a slide according to a first embodiment of the present utility model;

fig. 5 shows a schematic diagram of a detection device according to a second embodiment of the present utility model.

Wherein the above figures include the following reference numerals:

10. a glass slide; 11. a liquid inlet; 12. a liquid outlet;

20. a cover slip;

30. a bonding layer; 31. the contour of the runner is opened;

40. a flow channel cavity; 41. a liquid inlet channel; 411. a liquid inlet channel layer; 42. a dyeing chamber; 43. a liquid outlet channel; 431. a liquid outlet channel layer; 44. a liquid inlet cavity; 45. a liquid outlet cavity;

50. a positioning plate; 51. positioning the opening; 52. positioning holes;

60. a sample; 71. a fluid storage member; 711. a fluid tank; 712. a liquid inlet piece; 72. a flow channel chip; 73. a negative pressure member; 74. a waste liquid recovery member; 741. a recovery member; 742. a waste liquid tank.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1 to 4, a first embodiment of the present utility model provides a runner chip, the runner chip includes a slide glass 10, a cover glass 20, and an adhesive layer 30, the cover glass 20 is covered on the slide glass 10, the adhesive layer 30 is disposed between the slide glass 10 and the cover glass 20, the adhesive layer 30 has a runner contour opening 31 disposed therethrough, an inner sidewall of the runner contour opening 31, an upper surface of the slide glass 10, and a lower surface of the cover glass 20 collectively define a runner cavity 40, in a length direction of the slide glass 10, the runner cavity 40 includes a liquid inlet 41, a dyeing cavity 42, and a liquid outlet 43 that are sequentially connected, wherein the slide glass 10 or the cover glass 20 is disposed therethrough with a liquid inlet 11 that is connected with an inlet of the liquid inlet 41, the liquid inlet 41 includes a plurality of outlets that are disposed at intervals along a width direction of the slide glass 10, the liquid outlet 43 includes a plurality of inlets that are disposed at intervals along a width direction of the slide glass 42, the liquid outlet 43 is connected with an outlet of the dyeing cavity 42, and the slide glass 10 or the cover glass 20 is disposed with a liquid outlet 43 that is connected with an outlet of the slide glass 12.

The flow channel chip of this embodiment includes a slide glass 10, a cover glass 20 and a bonding layer 30, the inner side wall of a flow channel outline opening 31, the upper surface of the slide glass 10 and the lower surface of the cover glass 20 together enclose a flow channel cavity 40, in the length direction of the slide glass 10, the flow channel cavity 40 includes a liquid inlet channel 41, a staining cavity 42 and a liquid outlet channel 43 which are sequentially communicated, a sample 60 is placed on the slide glass 10, the sample 60 is exposed in the staining cavity 42, a fluorescent staining reagent is added to the inlet of the liquid inlet channel 41 through a liquid inlet on the slide glass 10 or the cover glass 20, the fluorescent staining reagent sequentially passes through the liquid inlet channel 41, the staining cavity 42 and the liquid outlet channel 43, and the fluorescent staining operation on the section of the sample 60 in the staining cavity 42 is completed, and then the sample 60 is discharged from the flow channel cavity 40 through the liquid outlet on the slide glass 10 or the cover glass 20. Therefore, by designing the shape of the channel profile opening 31, in the length direction of the slide glass 10, the channel cavity 40 includes the liquid inlet channel 41, the staining cavity 42 and the liquid outlet channel 43 which are sequentially communicated, the sample 60 is exposed in the staining cavity 42, and the area of the channel cavity 40 is reduced by utilizing the liquid inlet channel 41 and the liquid outlet channel 43 at two sides on the premise of ensuring that the sample 60 and the fluorescent staining agent are fully contacted, so that the use amount of the fluorescent staining agent is reduced, and the use cost of the reagent is saved.

Wherein the slide 10 and coverslip 20 are bonded with an adhesive layer 30 such that the flow channel chamber 40 is a closed fluid chamber with input and output ports. The reagent flow within staining cavity 42 may be laminar and highly uniform across the surface of sample 60 to achieve a gradient-free staining.

Moreover, since the plurality of outlets of the liquid inlet channel 41 are arranged at intervals along the width direction of the glass slide 10, when the fluorescent staining reagent flows into the staining cavity 42 from the liquid inlet channel 41, the fluorescent staining reagent is prevented from accumulating at the inlet of the staining cavity 42, the liquid at the inlet of the staining cavity 42 is prevented from accumulating, the plurality of inlets of the liquid outlet channel 43 are arranged at intervals along the width direction of the glass slide 10, when the fluorescent staining reagent flows into the liquid outlet channel 43 from the staining cavity 42, the fluorescent staining reagent is prevented from accumulating at the outlet of the staining cavity 42, on one hand, bubbles are prevented from being generated in the staining cavity 42, the liquid at the outlet of the flow channel cavity 40 is prevented from accumulating, so that the bubbles in the flow channel cavity 40 can be fully discharged, the later imaging observation is facilitated, on the other hand, the fluorescent staining reagent at the inlet of the staining cavity 42 is uniformly distributed along the width direction of the glass slide 10, the flow rate of the fluorescent staining reagent at the outlet of the staining cavity 42 is further uniform, the liquid exchange is more thorough, and the staining is more uniform.

Wherein the sample 60 comprises a biological tissue or cell sample 60.

Specifically, the thickness of the adhesive layer 30 may be selected according to the thickness of the actual sample 60, the size of the area of the staining cavity 42 may be changed according to the size of the sample 60, and the staining cavity 42 with a proper area size is beneficial to save reagent cost, and the sample 60 is placed on the slide 10 at a position corresponding to the staining cavity 42, so that the sample 60 is completely exposed to the staining cavity 42, so as to ensure that the sample 60 is stained after the reagent enters the staining cavity 42.

In this embodiment, the outer dimensions of the glass slide 10, the cover glass 20 and the adhesive layer 30 are 25mm x 75mm, the adhesive layer 30 is made of double faced adhesive tape, and the cover glass is made of a high-transmittance glass slide, so as to ensure good imaging effect after the sample 60 is dyed.

As shown in fig. 2, the liquid inlet channel 41 and the liquid outlet channel 43 are both tree-shaped structures. The fluorescent dyeing reagent is split for multiple times through the liquid inlet channel 41 with the tree structure, and the fluorescent dyeing reagent is converged for multiple times through the liquid outlet channel 43 with the tree structure, so that the reagent flow rate at the inlet of the liquid inlet channel 41 is uniformly distributed to multiple outlets of the liquid inlet channel 41, the reagent flow rate at the outlet of the liquid outlet channel 43 is uniformly distributed to multiple inlets of the liquid outlet channel 43, the reagent flow rate among multiple outlets of the liquid inlet channel 41 is uniformly distributed in the width direction of the glass slide 10, the reagent flow rate among multiple inlets of the liquid outlet channel 43 is uniformly distributed, the flow rate distribution of the fluorescent dyeing reagent in the dyeing cavity 42 is uniform, the liquid exchange is more thorough, and the dyeing is more uniform.

It should be noted that the tree structure refers to a multi-layer branched structure, and each layer includes a main runner and a plurality of branched runners formed by branching the main runner, where the main runner of each layer is a branched runner of the previous layer.

As shown in fig. 2, along the length direction of the slide glass 10, the liquid inlet channel 41 includes N liquid inlet channel layers 411 that are sequentially connected, where N is a positive integer greater than or equal to 2, and the nth liquid inlet channel layer 411 has 2 ^N-1 The nth inlet channel layer 411 has 2 ^N And a plurality of outlets. That is, in the present embodiment, along the length direction of the slide glass 10, from the inlet of the liquid inlet channel 41 to the outlet of the liquid inlet channel 41, the liquid inlet channel 41 is branched into two channels by one channel, and is branched into four channels by two channels by one channel, and so on, from 2 ^N-1 The flow passage is divided into two branches into 2 ^N And a strip flow channel.

By adopting the above split-into-split manner, the reagent flow rate at each inlet of each liquid inlet channel layer 411 is uniformly distributed to two outlets formed by splitting the inlet, so that the reagent flow rate at the inlet of the liquid inlet channel 41 is uniformly distributed to a plurality of outlets of the liquid inlet channel 41, and the reagent flow rate between a plurality of outlets of the liquid inlet channel 41 is further uniformly distributed in the width direction of the glass slide 10, so that the flow rate distribution of fluorescent staining reagent in the staining cavity 42 is uniform, the liquid exchange is more thorough, and the staining is more uniform.

As shown in fig. 2, along the length direction of the slide glass 10, the liquid outlet channel 43 includes N liquid outlet channel layers 431 sequentially connected, where N is a positive integer greater than or equal to 2, and the nth liquid outlet channel layer 431 has 2 ^N-1 The nth liquid outlet channel layer 431 has 2 ^N And a plurality of inlets. That is, in the present embodiment, along the length direction of the slide glass 10, from the outlet of the liquid outlet channel 43 to the inlet of the liquid outlet channel 43, the liquid outlet channel 43 is branched into two channels by one channel, and is branched into four channels by two channels by one channel, and so on, from 2 ^N-1 The flow passage is divided into two branches into 2 ^N And a strip flow channel.

By adopting the above split-split manner, the reagent flow rate at each outlet of each liquid outlet channel layer 431 is uniformly distributed to two inlets formed by splitting the outlet, so that the reagent flow rate at the outlet of the liquid outlet channel 43 is uniformly distributed to a plurality of inlets of the liquid outlet channel 43, and the reagent flow rate between the plurality of inlets of the liquid outlet channel 43 is further uniformly distributed in the width direction of the glass slide 10, so that the flow rate distribution of the fluorescent staining reagent in the staining cavity 42 is uniform, the liquid exchange is more thorough, and the staining is more uniform.

In this embodiment, the branch flow passages of the same level of the tree structure have the same pipe diameter. Because the pipe diameters of the branch flow passages of the same level of the tree structure are the same, the flow rates of a plurality of branch flow passages formed by the branching of the same main flow passage of the tree structure are the same, the flow rates of the reagents at a plurality of outlets of the liquid inlet channel 41 are the same, the flow rates of the reagents at a plurality of inlets of the liquid outlet channel 43 are the same, the flow rates of the reagents at the inlets of the dyeing cavity 42 are kept consistent in the width direction of the glass slide 10, the flow rates of the reagents at the outlets of the dyeing cavity 42 are kept consistent in the width direction of the glass slide 10, the flow rate distribution of fluorescent dyeing reagents in the dyeing cavity 42 is even, the liquid exchange is more thorough, and the dyeing is more uniform.

As shown in fig. 2, the flow channel chamber 40 has a central symmetrical structure. Because the flow channel cavity 40 is of a central symmetry structure, the flow velocity of the outlets of the liquid inlet channel 41 is the same as the flow velocity of the outlets of the liquid outlet channel 43, and the number of the outlets of the liquid inlet channel 41 and the number of the inlets of the liquid outlet channel 43 are the same, so that the fluorescent staining reagent in the staining cavity 42 flows along the length direction of the glass slide, the flow velocity distribution of the fluorescent staining reagent in the staining cavity 42 is uniform, the liquid exchange is more thorough, and the staining is more uniform.

As shown in fig. 2, the flow channel cavity 40 further includes a liquid inlet cavity 44 and a liquid outlet cavity 45, the liquid inlet 11 is communicated with the inlet of the liquid inlet channel 41 through the liquid inlet cavity 44, and the liquid outlet 12 is communicated with the outlet of the liquid outlet channel 43 through the liquid outlet cavity 45. When the reagent is injected into the flow channel cavity through the liquid inlet 11, the reagent is buffered by the liquid inlet cavity 44, so that the reagent can stably enter the liquid inlet channel 41, and when the reagent in the flow channel cavity 40 is discharged outside through the liquid outlet 12, the reagent is buffered by the liquid outlet cavity 45, so that the reagent can be stably discharged, and the flow rate of the reagent in the dyeing cavity 42 is stable.

As shown in fig. 4, a plurality of liquid inlets 11 are provided on the slide glass 10 at intervals in the width direction thereof, and a plurality of liquid outlets 12 are provided on the slide glass 10 at intervals in the width direction thereof. The liquid inlet 11 is used for inputting reagent, and the liquid outlet 12 is used for discharging reagent. The arrangement of 4 liquid inlets 11 and 4 liquid outlets 12 shown in fig. 4 is to be compatible with some specific equipment interfaces, and in practice, other numbers of liquid inlets 11 and liquid outlets 12 can be arranged as required.

In this embodiment, the multiple liquid inlets 11 are the same in size, and the multiple liquid outlets 12 are the same in size, so that when different reagents are injected through different liquid inlets 11, the number of different reagent sequences is reduced, reagent cost is saved, and the flow channel cavity 40 is also suitable for multiple staining.

As shown in fig. 2, the liquid inlet chamber 44 and the liquid outlet chamber 45 are each in a bar-shaped structure extending in the width direction of the slide 10. By adopting the strip-shaped structure, when the liquid inlet cavity 44 and the liquid outlet cavity 45 are utilized to buffer the reagent, the buffer effect can be improved, and when the types of the reagents injected by the liquid inlets 11 are different, the liquid inlet cavity 44 of the strip-shaped structure can be utilized to mix different reagents, so that the uniformly mixed reagents enter the liquid inlet channel 41, and the dyeing effect of the reagents in the dyeing cavity 42 is improved. It should be noted that the plurality of liquid inlets 11 may be filled with the same reagent.

As shown in fig. 3, the runner chip further includes a positioning plate 50, a positioning opening 51 is provided on the positioning plate 50, the slide glass 10, the cover glass 20 and the adhesive layer 30 are all embedded in the positioning opening 51, a positioning hole 52 is provided on the positioning plate 50, and the positioning hole 52 is located at the edge of the positioning opening 51. The slide glass 10, the cover glass 20 and the bonding layer 30 are embedded in the positioning opening 51, and then the positioning hole 52 on the positioning plate 50 is aligned with the positioning bulge on the detection device, so that the positioning and the installation of the runner chip on the detection device are completed, the liquid inlet and the liquid outlet of the runner chip can respectively correspond to the corresponding hole positions of the liquid inlet piece 712 and the recycling piece 741 of the detection device, liquid leakage is prevented, and the tightness of the runner cavity 40 is enhanced.

Specifically, in the assembly process of the flow channel chip 72, the sample 60 is adhered to the slide glass 10, one side of the adhesive layer 30 is adhered to the slide glass 10, the cover glass 20 is adhered to the other side of the adhesive layer 30, and the three adhered pieces are clamped into the positioning openings 51 of the positioning plate 50 to complete the assembly.

As shown in fig. 1 to 5, a second embodiment of the present utility model provides a detection device, which includes a fluid storage member 71, a flow channel chip 72, a negative pressure member 73 and a waste liquid recovery member 74, wherein a liquid inlet 11 of the flow channel chip 72 is connected to an outlet of the fluid storage member 71, the negative pressure member 73 is connected to a liquid outlet 12 of the flow channel chip 72, an inlet of the waste liquid recovery member 74 is connected to the liquid outlet 12 of the flow channel chip 72, and the flow channel chip 72 is provided as described above. Therefore, the detection device provided by the embodiment can also design the shape of the runner outline opening 31, in the length direction of the glass slide 10, so that the runner cavity 40 comprises the liquid inlet channel 41, the staining cavity 42 and the liquid outlet channel 43 which are sequentially communicated, the sample 60 is exposed in the staining cavity 42, and the area of the runner cavity 40 is reduced by utilizing the liquid inlet channel 41 and the liquid outlet channel 43 on two sides on the premise that the sample 60 and the fluorescent staining agent are fully contacted, so that the use amount of the fluorescent staining agent is reduced, and the use cost of the agent is saved.

As shown in fig. 5, the negative pressure member 73 communicates with the liquid outlet 12 of the flow channel chip 72. Because the negative pressure piece 73 is communicated with the liquid outlet 12 of the runner chip 72, the pressure of the liquid outlet 12 of the runner chip 72 is smaller than the pressure of the liquid inlet 11 of the runner chip 72 to form a pressure difference, so that the negative pressure piece 73 provides negative pressure driving for the runner chip 72 to drive the reagent in the runner cavity 40 to flow from the liquid inlet 11 to the liquid outlet 12.

As shown in fig. 5, the inlet of the waste liquid recovery member 74 communicates with the liquid outlet 12 of the flow channel chip 72. The waste liquid recovery piece 74 comprises a waste liquid tank 742 and a recovery piece 741, the liquid outlet 12 is communicated with the inlet of the recovery piece 741, the outlet of the recovery piece 741 is communicated with the waste liquid tank 742, and the recovery piece 741 is used for recovering the reagent in the liquid outlet 12 into the waste liquid tank 742, so that pollution caused by waste liquid is avoided.

Specifically, the negative pressure member 73 communicates with the recovery member 741, and the recovery member 741 communicates with the liquid outlet 12.

As shown in fig. 5, the fluid storage member 71 includes a fluid tank 711 and a liquid inlet member 712, the fluid tank 711 is communicated with the inlet of the liquid inlet member 712, the outlet of the liquid inlet member 712 is communicated with the liquid inlet port 11, the fluid tank 711 is used to store reagents such as fluorescent dye and buffer solution, and the liquid inlet member 712 is used to inject the reagents in the fluid tank 711 into the liquid inlet port 11.

In this embodiment, the operation steps of the detection device are as follows:

(1) Applying negative pressure driving to the recovery member 741 by the negative pressure member 73, and introducing the reagent from the fluid tank 711 into the liquid inlet member 712;

(2) Reagents enter the flow channel cavity 40 of the flow channel chip 72 from the liquid inlet 11 of the flow channel chip 72 from the liquid inlet 712, and the reagents dye the sample 60;

(3) The reagent flows out from the liquid outlet 12 of the flow channel chip 72 to the recovery piece 741;

(4) The reagent flows from the recovery piece 741 to the waste liquid tank 742, and one operation of the detecting device is completed.

In this embodiment, the detection device is a sequencer, and by customizing a fluorescent staining protocol for the sequencer, the fluorescent staining protocol includes image acquisition, temperature control, reagent exchange and stable positioning, so as to implement iterative indirect fluorescent staining imaging of a complex multi-day continuous workflow under the unattended condition, and finally construct a high-level multipath diagram of cell types and pathological features of animals or plants after a plurality of cycles of staining, imaging and antibody elution.

The technical scheme provided by the embodiment has the following beneficial effects:

(1) By designing the shape of the runner contour opening 31, the runner cavity 40 comprises a liquid inlet channel 41, a dyeing cavity 42 and a liquid outlet channel 43 which are sequentially communicated in the length direction of the glass slide 10, the sample 60 is exposed in the dyeing cavity 42, and the area of the runner cavity 40 is reduced by utilizing the liquid inlet channel 41 and the liquid outlet channel 43 on two sides on the premise of ensuring that the sample 60 and the fluorescent dye are fully contacted, so that the use amount of the fluorescent dye is reduced, and the use cost of the reagent is saved;

(2) The adoption of the tree structure divided into two parts ensures that the reagent flow rates among a plurality of outlets of the liquid inlet channel 41 are uniformly distributed in the width direction of the glass slide 10, and the reagent flow rates among a plurality of inlets of the liquid outlet channel 43 are uniformly distributed, so that the flow rate distribution of fluorescent staining reagent in the staining cavity 42 is uniform, the liquid exchange is more thorough, and the staining is more uniform;

(3) The liquid inlets 11 and the liquid outlets 12 are adopted, so that the number of the liquid inlets 11 and the liquid outlets 12 can be set according to the requirements of being compatible with certain specific equipment interfaces, and the equipment compatibility is improved;

(4) Since the outlets of the liquid inlet channel 41 are arranged at intervals along the width direction of the glass slide 10, and the inlets of the liquid outlet channel 43 are arranged at intervals along the width direction of the glass slide 10, the flow velocity of the fluorescent staining reagent in the staining cavity 42 is distributed uniformly, the flow field is more stable, the liquid exchange is more thorough, and the staining is more uniform.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present utility model unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

In the description of the present utility model, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, merely to facilitate description of the present utility model and simplify the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of protection of the present utility model; the orientation word "inner and outer" refers to inner and outer relative to the contour of the respective component itself.

Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

In addition, the terms "first", "second", etc. are used to define the components, and are only for convenience of distinguishing the corresponding components, and the terms have no special meaning unless otherwise stated, and therefore should not be construed as limiting the scope of the present utility model.

The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (10)

1. A flow channel chip, characterized in that the flow channel chip comprises:

a slide (10);

a cover glass (20) which is arranged on the glass slide (10) in a covering way;

the adhesive layer (30) is arranged between the glass slide (10) and the cover glass (20), the adhesive layer (30) is provided with a runner outline opening (31) which is arranged in a penetrating manner, the inner side wall of the runner outline opening (31), the upper surface of the glass slide (10) and the lower surface of the cover glass (20) jointly enclose a runner cavity (40), and the runner cavity (40) comprises a liquid inlet channel (41), a dyeing cavity (42) and a liquid outlet channel (43) which are communicated in sequence in the length direction of the glass slide (10);

the glass slide device comprises a glass slide (10) and a cover glass (20), wherein a liquid inlet (11) communicated with an inlet of the liquid inlet channel (41) is formed in the glass slide (10) or the cover glass (20) in a penetrating mode, the liquid inlet channel (41) comprises a plurality of outlets which are arranged at intervals in the width direction of the glass slide (10), the outlets of the liquid inlet channel (41) are communicated with an inlet of a dyeing cavity (42), the liquid outlet channel (43) comprises a plurality of inlets which are arranged at intervals in the width direction of the glass slide (10), the inlets of the liquid outlet channel (43) are communicated with an outlet of the dyeing cavity (42), and the glass slide (10) or the cover glass (20) is provided with a liquid outlet (12) which is communicated with an outlet of the liquid outlet channel (43) in a penetrating mode.

2. The flow channel chip according to claim 1, wherein the liquid inlet channel (41) and the liquid outlet channel (43) are both tree-like structures.

3. The flow channel chip according to claim 2, wherein the liquid inlet channel (41) includes N liquid inlet flow channel layers (411) which are sequentially communicated in a length direction of the slide glass (10);

wherein N is a positive integer greater than or equal to 2, and the Nth liquid inlet flow channel layer (411) has 2 ^N-1 The Nth inlet channel layer (411) has 2 ^N And a plurality of outlets.

4. The flow channel chip according to claim 2, wherein the liquid outlet channel (43) includes N liquid outlet channel layers (431) that are sequentially communicated in a length direction of the slide glass (10);

wherein N is a positive integer greater than or equal to 2, and the Nth liquid outlet channel layer (431) has 2 ^N-1 The Nth liquid outlet channel layer (431) has 2 ^N And a plurality of inlets.

5. The flow channel chip according to claim 2, wherein the branch flow channels of the same level of the tree structure have the same pipe diameter.

6. The flow channel chip according to any one of claims 1 to 5, characterized in that the flow channel cavity (40) is of a centrosymmetric structure.

7. The flow channel chip according to any one of claims 1 to 5, wherein the flow channel cavity (40) further comprises a liquid inlet cavity (44) and a liquid outlet cavity (45), the liquid inlet (11) is communicated with an inlet of the liquid inlet channel (41) through the liquid inlet cavity (44), and the liquid outlet (12) is communicated with an outlet of the liquid outlet channel (43) through the liquid outlet cavity (45).

8. The flow channel chip as claimed in claim 7, wherein,

a plurality of liquid inlets (11) are formed in the glass slide (10) at intervals along the width direction of the glass slide, and a plurality of liquid outlets (12) are formed in the glass slide (10) at intervals along the width direction of the glass slide; and/or the number of the groups of groups,

the liquid inlet cavity (44) and the liquid outlet cavity (45) are both strip-shaped structures extending along the width direction of the glass slide (10).

9. The flow channel chip as claimed in claim 7, wherein,

the runner chip further comprises a positioning plate (50), a positioning opening (51) is formed in the positioning plate (50), and the glass slide (10), the cover glass (20) and the bonding layer (30) are embedded in the positioning opening (51);

the locating plate (50) is provided with a locating hole (52), and the locating hole (52) is positioned at the edge of the locating opening (51).

10. A detection device, characterized in that the detection device comprises a fluid storage part (71), a runner chip (72), a negative pressure part (73) and a waste liquid recovery part (74), wherein a liquid inlet (11) of the runner chip (72) is communicated with an outlet of the fluid storage part (71), the negative pressure part (73) is communicated with a liquid outlet (12) of the runner chip (72), an inlet of the waste liquid recovery part (74) is communicated with the liquid outlet (12) of the runner chip (72), and the runner chip (72) is the runner chip according to any one of claims 1 to 9.

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