CN218910349U - Collector and cell sorting equipment - Google Patents

Abstract

The utility model relates to a collector and cell sorting equipment, the collector includes: the shell is provided with a mixing bin and a sample adding bin, and a filling hole communicated with the mixing bin is formed in the shell; the microfluidic chip is arranged on the shell; the collection container is detachably connected with the shell, and the target cell solution separated by the microfluidic chip can flow into the collection container; the inlet of the microfluidic chip, the sample adding bin and the collecting container are respectively and selectively communicated with the mixing bin through a communication structure. After the micro-fluidic chip discharges the target cell solution separated for the first time into the collecting container, the communicating structure enables the collecting container to be communicated with the mixing bin, negative pressure is input into the mixing bin through the filling hole, and the target cell solution in the collecting container is sucked into the mixing bin. And the communication structure enables the microfluidic chip to be communicated with the mixing bin, positive pressure is input into the mixing bin through the filling hole, so that target cell solution in the mixing bin is pressed into the microfluidic chip, and secondary separation is carried out through the microfluidic chip.

Description

Collector and cell sorting equipment

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a collector and cell sorting equipment.

Background

Cell sorting is a technique for separating target cells from a sample, and the cell sorting process is performed by a collector.

The collector comprises a microfluidic chip and a collecting container, and after the sample is injected into the microfluidic chip, the microfluidic chip can separate out target cells and collect the target cells through the collecting container. The patent with publication number CN 112159752A discloses a cell separation device, which comprises a sample input device, a microfluidic chip box and a sample collection device, wherein the sample collection device comprises a support, a sample collection bracket and a waste liquid collection box which are arranged in the support, and a sample collection pipe is arranged in the sample collection bracket. The sample input device is used for injecting a sample into the microfluidic chip of the microfluidic chip box. The sample collecting pipe is used for collecting target cells discharged by the microfluidic chip in the microfluidic chip box. The research result of the prior art shows that the background impurities can be removed better by carrying out secondary separation on the sample, and the solution of the target cells with higher purity can be obtained. However, in the above-mentioned cell separation device, in order to perform secondary separation of the solution of the target cells in the sample collection device, the sample collection tube needs to be first taken out from the sample collection rack, then the solution of the target cells in the sample collection tube is injected into the sample input device, and then the solution is injected into the microfluidic chip through the sample input device, so that the process of performing secondary separation on the sample is complex, resulting in low cell separation efficiency and possible pollution to the sample in the operation process.

Therefore, a collector is needed to solve the above technical problems.

Disclosure of Invention

It is an object of the present utility model to provide a harvester for harvesting a secondarily separated target cell solution.

To achieve the above object, a first aspect of the present utility model provides a collector, including:

the device comprises a shell, wherein a mixing bin and a sample adding bin are arranged in the shell, and a filling hole communicated with the mixing bin is formed in the shell;

the microfluidic chip is arranged on the shell;

a collection container detachably connected to the housing, into which the target cell solution separated by the microfluidic chip can flow;

the inlet of the microfluidic chip, the sample adding bin and the collecting container are respectively and selectively communicated with the mixing bin through a communication structure.

Optionally, the communication structure comprises a sample adding bin connecting hose, a chip connecting hose and a collecting container connecting hose;

the sample adding bin is communicated with the mixing bin through the sample adding bin connecting hose, the inlet of the microfluidic chip is communicated with the mixing bin through the chip connecting hose, and the collecting container is communicated with the mixing bin through the collecting container connecting hose;

the shell is provided with an avoidance port which enables the connecting hose of the sample loading bin, the connecting hose of the chip and the connecting hose of the collecting container to be at least partially exposed.

Optionally, a chip connecting hole is formed in the shell, one end of the chip connecting hole is communicated with the lower end of the mixing bin, and the other end of the chip connecting hole is connected with the chip connecting hose.

Optionally, a sample adding bin connecting hole and a collecting container connecting hole near the upper end of the sample adding bin are formed in the shell, the sample adding bin connecting hose is communicated with the mixing bin through the sample adding bin connecting hole, and the collecting container connecting hose is connected with the mixing bin through the collecting container connecting hole.

Optionally, the lateral wall of casing is last to be offered and is used for holding respectively loading storehouse coupling hose chip coupling hose with collecting vessel coupling hose's holding tank, one the holding tank with dodge the mouth intercommunication.

Optionally, a side wall of the shell comprises an inner plate and a cover plate, the accommodating groove is formed in one side, close to the cover plate, of the inner plate, and the avoidance opening penetrates through the cover plate.

Optionally, an accommodating groove is formed in the outer side wall of the shell, and the collecting container is arranged in the accommodating groove.

Optionally, the collector further comprises a cover body, the cover body can be covered on the collecting container, a clamping groove is formed in the outer side wall of the shell, and the cover body is clamped in the clamping groove.

Optionally, a plugging slot for fixing the collector is formed on the side wall of the shell.

It is another object of the present utility model to provide a cell sorting apparatus for collecting a secondarily separated target cell solution.

To achieve the object, the second aspect of the present utility model adopts the following technical scheme:

a cell sorting device comprises the collector.

From the above, according to the technical scheme provided by the utility model, after the microfluidic chip discharges the target cell solution separated for the first time into the collecting container, the collecting container is communicated with the mixing bin by the communication structure, negative pressure is input into the mixing bin through the filling hole, and the target cell solution in the collecting container is sucked into the mixing bin. And the communication structure enables the microfluidic chip to be communicated with the mixing bin, positive pressure is input into the mixing bin through the filling hole, so that target cell solution in the mixing bin is pressed into the microfluidic chip, and secondary separation is carried out through the microfluidic chip.

Drawings

FIG. 1 is a schematic diagram of a collector according to an embodiment of the present utility model;

FIG. 2 is a schematic view of the structure in a housing according to an embodiment of the present utility model;

FIG. 3 is a cross-sectional view of a harvester according to an embodiment of the utility model;

FIG. 4 is a schematic view of the structure of the collector with the cover plate and hose removed;

fig. 5 is a schematic structural diagram of another view angle of the collector according to the embodiment of the present utility model.

In the figure:

1. a housing; 11. a mixing bin; 12. a sample adding bin; 13. filling the hole; 14. a chip connection hole; 15. a sample adding bin connecting hole; 16. a collection container connection hole; 17. an inner plate; 171. a receiving groove; 18. a cover plate; 19. a waste liquid bin; 101. a clamping groove; 102. a plug-in groove; 103. a first seal ring; 104. a second seal ring; 105. an accommodating groove;

2. a microfluidic chip;

3. a collection container;

4. a communication structure; 41. the sample adding bin is connected with a hose; 42. the chip is connected with the hose; 43. the collecting container is connected with a hose;

5. an avoidance port; 6. an upper cover; 7. and a cover body.

Detailed Description

The technical scheme of the utility model is further described below by the specific embodiments with reference to the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the utility model and are not limiting thereof. It should be further noted that, for convenience of description, only some, but not all of the drawings related to the present utility model are shown.

In the present utility model, directional terms such as "upper", "lower", "left", "right", "inner" and "outer" are used for convenience of understanding, and thus do not limit the scope of the present utility model unless otherwise specified.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

The embodiment provides a collector for separating and collecting target cells, so as to improve the cell sorting efficiency and reduce the sample pollution probability.

As shown in fig. 1 and 2, the collector provided in this embodiment includes a housing 1, a microfluidic chip 2, and a collection container 3. A mixing bin 11 and a sample adding bin 12 are arranged in the shell 1, and a filling hole 13 communicated with the mixing bin 11 is formed in the shell 1. The microfluidic chip 2 is disposed in the housing 1, and the microfluidic chip 2 is used for separating target cells in a sample, and the specific structure and working principle of the microfluidic chip 2 are the prior art, so that details are not repeated here. The collection container 3 is detachably connected to the housing 1, and the target cell solution separated by the microfluidic chip 2 can flow into the collection container 3, and the collection container 3 can be a test tube or a centrifuge tube. The inlet of the microfluidic chip 2, the sample loading bin 12 and the collection container 3 are selectively communicated with the mixing bin 11 through the communication structure 4 respectively.

The filling hole 13 is communicated with the mixing bin 11, and the cell sorting equipment can inject the rinse solution and/or the diluent into the mixing bin 11 through the filling hole 13, and can also input positive pressure or negative pressure into the mixing bin 11 through the filling hole 13, namely, gas is filled into the mixing bin 11, so that the gas pressure in the mixing bin 11 is greater than the atmospheric pressure to form positive pressure, or the gas in the mixing bin 11 is extracted, so that the gas pressure in the mixing bin 11 is less than the atmospheric pressure to form negative pressure.

When the solution in the mixing bin 11 is required to be injected into the microfluidic chip 2, the mixing bin 11 is communicated with the microfluidic chip 2 through the communication structure 4, and positive pressure is input into the mixing bin 11 through the filling hole 13 so as to press the solution in the mixing bin 11 into the microfluidic chip 2. When the solution in the sample adding bin 12 or the collecting container 3 is required to be injected into the mixing bin 11, the mixing bin 11 is communicated with the sample adding bin 12 or the collecting container 3 through the communication structure 4, and negative pressure is input into the mixing bin 11 through the filling hole 13 so as to suck the solution in the sample adding bin 12 or the collecting container 3 into the mixing bin 11.

After the microfluidic chip 2 discharges the target cell solution separated for the first time into the collecting container 3, the communicating structure 4 enables the collecting container 3 to communicate with the mixing bin 11, negative pressure is input into the mixing bin 11 through the filling hole 13, and the target cell solution in the collecting container 3 is sucked into the mixing bin 11. Then, the communication structure 4 communicates the microfluidic chip 2 with the mixing bin 11, and positive pressure is input into the mixing bin 11 through the filling hole 13 so as to press the target cell solution in the mixing bin 11 into the microfluidic chip 2, thereby performing secondary separation through the microfluidic chip 2.

Meanwhile, the collector provided by the embodiment can provide samples for the mixing bin 11 through the communication structure and positive and negative press fit in the mixing bin 11, the samples can enter the microfluidic chip 2 after being diluted in the mixing bin 11, and the sample adding operation of the microfluidic chip 2 is realized, so that the microfluidic chip 2 is subjected to sample adding without an additional device, the structure of the sampler is simplified, and the cost of the sampler is reduced.

As shown in fig. 1, the communication structure 4 optionally includes a loading bay connection hose 41, a chip connection hose 42, and a collection container connection hose 43. The sample adding bin 12 is communicated with the mixing bin 11 through a sample adding bin connecting hose 41, the inlet of the microfluidic chip 2 is communicated with the mixing bin 11 through a chip connecting hose 42, and the collecting container 3 is communicated with the mixing bin 11 through a collecting container connecting hose 43. The shell 1 is provided with a avoiding opening 5 which exposes at least part of the sample loading bin connecting hose 41, the chip connecting hose 42 and the collecting container connecting hose 43. Optionally, a relief opening 5 corresponds to a hose for operating the respective hose. Specifically, the user can pass through the avoiding port 5 by hand or by a hand or the like on the cell sorting apparatus, and press the sample well connection hose 41, the chip connection hose 42, or the collection container connection hose 43, thereby preventing the solution from passing therethrough, and release the sample well connection hose 41, the chip connection hose 42, or the collection container connection hose 43, thereby allowing the solution to pass therethrough.

Alternatively, the loading bay connecting hose 41 may be in communication with the lower end of the loading bay 12, thereby minimizing or avoiding solution residue within the loading bay 12. The collection container connection hose 43 may be in communication with the lower end of the collection container 3 to minimize or avoid solution residue in the collection container 3.

In another embodiment, the communication structure 4 may include a pipeline and a valve connected to the pipeline, and the valve may control opening and closing of the pipeline. The tubing may be a rigid tube having a fixed shape, such as tubing made of plastic or metal. The valve is a manual valve, such as a rotary valve or a passage valve, and is positioned in the avoiding port 5, so that the valve can be opened and closed manually or by a clamping hand on the cell sorting equipment; or the valve is an automatic control valve, such as an electromagnetic valve, and the opening and the closing of the valve are controlled by a control system, so that the inlet of the microfluidic chip 2, the sample adding bin 12 and the collecting container 3 are respectively and selectively communicated with the mixing bin 11.

As shown in fig. 2, optionally, a waste liquid bin 19 may be further disposed in the housing 1, where the waste liquid bin 19 is in communication with the waste liquid outlet of the microfluidic chip 2, so as to discharge waste liquid into the waste liquid bin 19.

As shown in fig. 3, the microfluidic chip 2 may be located above the waste bin 19 and the collection container 3, thereby facilitating the discharge of the waste liquid and the target cell solution.

In order to communicate the chip connection hose 42 with the mixing chamber 11, optionally, a chip connection hole 14 is formed in the housing 1, one end of the chip connection hole 14 is communicated with the lower end of the mixing chamber 11, so that the solution in the mixing chamber 11 is drained, and the other end of the chip connection hole 14 is connected with the chip connection hose 42, so that the mixing chamber 11 is communicated with the microfluidic chip 6.

Optionally, the lower end of the mixing chamber 11 is tapered to avoid residual solution at the bottom of the mixing chamber 11.

Optionally, a first sealing ring 103 is connected to the inner wall of the filling hole 13, so that leakage is avoided when the mixing chamber 11 is ventilated or liquid is ventilated. The upper end of the waste liquid bin 19 is provided with an upper cover 6, and a second sealing ring 104 is arranged between the upper cover 6 and the waste liquid bin 19, so that positive pressure or negative pressure in the waste liquid bin 19 is maintained.

As shown in fig. 3 and 4, the casing 1 is provided with a loading chamber connecting hole 15 and a collecting container connecting hole 16, a loading chamber connecting hose 41 is communicated with the mixing chamber 11 through the loading chamber connecting hole 15, and a collecting container connecting hose 43 is connected with the mixing chamber 11 through the collecting container connecting hole 16. Alternatively, the loading chamber connecting hole 15 and the collecting container connecting hole 16 are both disposed near the upper end of the mixing chamber 11, and after the solution in the mixing chamber 11 is added, the loading chamber connecting hole 15 and the collecting container connecting hole 16 can be both located on the upper side of the liquid surface, thereby avoiding uncontrolled entry of the solution in the mixing chamber 11 into the loading chamber connecting hose 41 and the collecting container connecting hose 43.

Optionally, a filling hole 13 is also provided near the upper end of the mixing chamber 11, so that when a solution is provided in the mixing chamber 11, the gas above the solution can be evacuated or the pressure above the solution can be increased. Further, the filling hole 13, the sample well connecting hole 15 and the collection container connecting hole 16 are located at the same height.

As shown in fig. 4, the outer side wall of the housing 1 is provided with receiving grooves 171 for receiving the loading chamber connecting hose 41, the chip connecting hose 42 and the collecting container connecting hose 43, respectively, and one receiving groove 171 communicates with one avoiding port 5. The receiving groove 171 may limit the hose so as to prevent the hose from being bent and thus the solution from being impossible to circulate.

As shown in fig. 1 and 4, a side wall of the housing 1 includes an inner plate 17 and a cover plate 18, the accommodating groove 171 is formed on one side of the inner plate 17 close to the cover plate 18, the escape opening 5 penetrates the cover plate 18, the escape opening 5 may extend to the inner plate 17, and the escape opening 5 may or may not penetrate the inner plate 17, so long as the hose can be smoothly extended from the accommodating groove 171 to the escape opening 5. The cover 18 protects the hose from false touches. It will be appreciated that the inner plate 17 and the cover plate 18 are disposed along the wall thickness direction of the housing 1, and the inner plate 17 and the cover plate 18 may be connected by screws or by snap connection, and the specific connection manner of the inner plate 17 and the cover plate 18 is not particularly limited.

The chip connecting hole 14, the sample loading bin connecting hole 15 and the collection container connecting hole 16 may be formed in the inner plate 17.

The accommodating groove 171 and the avoiding opening 5 are formed in the same side wall of the housing 1, so that the cell sorting apparatus can conveniently control a plurality of hoses.

As shown in fig. 5, the opening of the loading bay 12 may be located at the upper end of the housing 1, so as to facilitate the loading of samples into the loading bay 12 by a user.

The housing 1 has an accommodating groove 105 formed in an outer side wall thereof, and the collection container 3 is disposed in the accommodating groove 105. Alternatively, the accommodating groove 105 is opened at the outer side of the outer sidewall so as to open the access accommodating groove 105. A receptacle may be provided in the receiving groove 105, into which the upper or lower end of the collection container 3 is inserted, thereby fixing the collection container 3. In other alternative embodiments, the collection container 3 may also be detachably connected in the receiving recess 105 by other means, without limitation.

The collector further comprises a cover body 7, the cover body 7 can be covered on the collecting container 3, a clamping groove 101 is formed in the outer side wall of the shell 1, and the cover body 7 is clamped in the clamping groove 101.

The side wall of the shell 1 is provided with a plugging groove 102 for fixing the collector. Alternatively, the insertion groove 102 extends in a vertical direction, and a guide structure may be further provided at a lower end of the insertion groove 102 to facilitate the protrusion to enter the insertion groove 102.

The working process of the collector provided by the embodiment is as follows:

1. sample adding: adding a sample into the sample adding bin 12, wherein the sample adding bin connecting hose 41, the chip connecting hose 42 and the collecting container connecting hose 43 are all in an open circuit state;

2. filling a rinse: filling the rinse solution into the mixing bin 11 through the filling hole 13;

3. rinsing the microfluidic chip 2: the chip connecting hose 42 is communicated with the microfluidic chip 2 and the mixing bin 11, positive pressure is input into the mixing bin 11 through the filling hole 13 so as to press the rinse solution in the mixing bin 11 into the microfluidic chip 2, and the solution in the microfluidic chip 2 is discharged to the waste liquid bin 19 or to the waste liquid bin 19 and the collecting container 3; the chip connection hose 42 is disconnected;

4. filling a diluent: filling the diluting liquid into the mixing bin 11 through the filling hole 13;

5. diluting the sample: the sample adding bin connecting hose 41 is communicated with the sample adding bin 12 and the mixing bin 11, and negative pressure is input into the mixing bin 11 through the filling hole 13 so that samples in the sample adding bin 12 are pumped into the mixing bin 11, and diluted sample solution is obtained; the connecting hose 41 of the sample loading bin is disconnected;

6. first separation of samples: the chip connecting hose 42 is communicated with the microfluidic chip 2 and the mixing bin 11, positive pressure is input into the mixing bin 11 through the filling hole 13, so that a sample solution in the mixing bin 11 is pressed into the microfluidic chip 2, the microfluidic chip 2 separates a sample solution, a target cell solution obtained by the first separation is discharged to the collecting container 3, and the rest solution is discharged to the waste liquid bin 19; the chip connection hose 42 is disconnected;

7. the first separated sample is returned to mixing chamber 11: the collection container connecting hose 43 is communicated with the collection container 3 and the mixing bin 11, negative pressure is input into the mixing bin 11 through the filling hole 13, so that the target cell solution in the collection container 3 flows back to the mixing bin 11 to prepare for the second separation of the sample; the collection container connection hose 43 is disconnected;

8. diluting the first separated sample: adding a diluent into the mixing bin 11 through the filling hole 13 to dilute the target cell solution in the mixing bin 11;

9. second separation of samples: the chip connecting hose 42 is communicated with the microfluidic chip 2 and the mixing bin 11, positive pressure is input into the mixing bin 11 through the filling hole 13, so that a sample solution in the mixing bin 11 is pressed into the microfluidic chip 2, the microfluidic chip 2 separates a sample solution, a target cell solution obtained by the second separation is discharged to the collecting container 3, and the rest solution is discharged to the waste liquid bin 19;

10. the collection container 3 is taken out, and the cover 7 is taken out and covered on the collection container 3.

The acquisition of the target cell of collector that this embodiment provided is through the pressure in the input mixing bin 11 to and the on-off control of communication structure 4, thereby realize the secondary separation of sample, simplified the operation flow greatly, improved separation efficiency, in the in-process of secondary separation, the sample can not leave the collector, thereby avoid the secondary pollution of sample.

The embodiment also provides a cell sorting apparatus including a collector. The cell sorting device may further comprise a device body, and the device body may further be provided with a plug block plugged into the plug slot 102, so that the collector is stably installed on the device body.

Optionally, the collector that this embodiment provided can be disposable consumptive material package, and the collector is as a overall structure, when carrying out cell separation, only need install this holistic collector on the equipment body can use, convenient operation, work efficiency is high. From the application of sample until the separation out the target cell solution through secondary separation all realize on the collector, to cell analysis equipment, there is not other material loss to need not dismantle a plurality of consumptive material packages, further improve work efficiency.

Optionally, the cell sorting apparatus further comprises a filling member insertable into the filling port, the filling member being adapted to fill the mixing chamber 11 with a rinse, a diluent or a gas or to aspirate at least part of the gas in the mixing chamber 11.

It can be understood that the number of the mixing bin 11, the sample adding bin 12, the microfluidic chip 2, the collecting container 3 and the waste liquid bin 19 provided in this embodiment may be one or more, and the number of the mixing bin 11, the sample adding bin 12, the microfluidic chip 2, the collecting container 3 and the waste liquid bin 19 may be equal or different, and may be selected as required, which is not particularly limited in this embodiment and is within the scope of the present utility model.

The embodiment also provides a cell collection method, which uses the collector or the cell sorting equipment, and the cell collection method comprises the following steps:

when the solution in the mixing bin 11 is required to be injected into the microfluidic chip 2, the mixing bin 11 is communicated with the microfluidic chip 2 through the communication structure 4, and positive pressure is input into the mixing bin 11 through the filling hole 13;

when the solution in the sample loading bin 12 or the collection container 3 is required to be injected into the mixing bin 11, the mixing bin 11 is communicated with the sample loading bin 12 or the collection container 3 through the communication structure 4, and negative pressure is input into the mixing bin 11 through the injection hole 13.

Alternatively, the amount of sample drawn in the sample compartment, the amount of solution drawn in the collection container 3, and the amount of solution drawn in the mixing compartment 11 may be controlled by time or flow rate, and are not limited herein.

The cell collection method provided by the embodiment realizes the secondary separation of the sample by inputting the pressure in the mixing bin 11 and controlling the on-off of the communication structure 4, greatly simplifies the operation flow, improves the separation efficiency, and prevents the sample from leaving the collector in the secondary separation process, thereby avoiding secondary pollution of the sample.

While the utility model has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the utility model and are intended to be within the scope of the utility model as claimed.

Claims (10)

1. A collector, comprising:

the device comprises a shell (1), wherein a mixing bin (11) and a sample adding bin (12) are arranged in the shell (1), and a filling hole (13) communicated with the mixing bin (11) is formed in the shell (1);

the micro-fluidic chip (2), the micro-fluidic chip (2) is arranged on the shell (1);

-a collection container (3), the collection container (3) being detachably connected to the housing (1), the target cell solution separated by the microfluidic chip (2) being able to flow into the collection container (3);

the inlet of the microfluidic chip (2), the sample adding bin (12) and the collecting container (3) are respectively and selectively communicated with the mixing bin (11) through a communication structure (4).

2. Collector according to claim 1, wherein the communication structure (4) comprises a loading bay connection hose (41), a chip connection hose (42) and a collection container connection hose (43);

the sample adding bin (12) is communicated with the mixing bin (11) through the sample adding bin connecting hose (41), the inlet of the microfluidic chip (2) is communicated with the mixing bin (11) through the chip connecting hose (42), and the collecting container (3) is communicated with the mixing bin (11) through the collecting container connecting hose (43);

the shell (1) is provided with an avoidance port (5) which enables the sample adding bin connecting hose (41), the chip connecting hose (42) and the collecting container connecting hose (43) to be at least partially exposed.

3. Collector according to claim 2, characterized in that the housing (1) is provided with a chip connecting hole (14), one end of the chip connecting hole (14) is communicated with the lower end of the mixing bin (11), and the other end of the chip connecting hole (14) is connected with the chip connecting hose (42).

4. Collector according to claim 2, characterized in that the housing (1) is provided with a loading bin connecting hole (15) and a collecting container connecting hole (16) near the upper end of the loading bin (12), the loading bin connecting hose (41) is communicated with the mixing bin (11) through the loading bin connecting hole (15), and the collecting container connecting hose (43) is connected with the mixing bin (11) through the collecting container connecting hole (16).

5. Collector according to claim 2, characterized in that the outer side wall of the housing (1) is provided with a containing groove (171) for containing the sample loading bin connecting hose (41), the chip connecting hose (42) and the collecting container connecting hose (43), respectively, and one containing groove (171) is communicated with one avoiding opening (5).

6. Collector according to claim 5, wherein a side wall of the housing (1) comprises an inner plate (17) and a cover plate (18), the accommodating groove (171) is formed on one side of the inner plate (17) close to the cover plate (18), and the avoidance opening (5) penetrates through the cover plate (18).

7. Collector according to claim 1, characterized in that the housing (1) is provided with a receiving recess (105) on its outer side wall, the collecting container (3) being arranged in the receiving recess (105).

8. The collector of claim 1, further comprising a cover body (7), wherein the cover body (7) can be covered on the collecting container (3), a clamping groove (101) is formed in the outer side wall of the shell (1), and the cover body (7) is clamped in the clamping groove (101).

9. Collector according to claim 1, characterized in that the side wall of the housing (1) is provided with a socket (102) for fixing the collector.

10. A cell sorting apparatus comprising a collector according to any one of claims 1 to 9.

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