CN218741282U

CN218741282U - centrifugal filter

Info

Publication number: CN218741282U
Application number: CN202223034827.3U
Authority: CN
Inventors: 刘小平; 陈智明; 陈高明; 胡玉梅
Original assignee: Biocomma Ltd
Current assignee: Biocomma Ltd
Priority date: 2022-11-15
Filing date: 2022-11-15
Publication date: 2023-03-28
Anticipated expiration: 2032-11-15

Abstract

The utility model provides a centrifugal filtering device, which comprises a filter and a collecting pipe; the filter comprises a filter base body and a filter membrane, the filter base body comprises a sample inlet opening arranged at a first end of the filter base body, a filter cavity communicated with the sample inlet opening, and a flow guide hole arranged on the filter base body and at a second end corresponding to the first end, and a dead volume chamber is arranged at the position, close to the second end, of the filter cavity; the filter membrane is arranged at the second end of the filter base body, an included angle is formed between the filter membrane and the axis of the filter base body, and the included angle is larger than 0 degree and smaller than 90 degrees; the collecting tube has a collecting chamber and a collecting opening, the filter base being detachably connected to the collecting tube. Because filter membrane and water conservancy diversion hole set up the same end at filtering the base member, simplified the structure of filter, reduced the precision requirement of each spare part assembly of device, reduced the processing degree of difficulty to improve production efficiency, reduced product manufacturing cost.

Description

Centrifugal filter

Technical Field

The utility model relates to a sample filtering separation technical field, concretely relates to centrifugal filter device.

Background

Centrifugal filters can be used to separate biological substances such as abzymes, nucleic acids and proteins for concentration, desalination, purification and fractionation purposes, such as concentrating urine, serum, plasma and cerebrospinal fluid. A conventional centrifugal filter typically comprises a housing with a sample container, a filter with a dead volume chamber sealed in the housing, and a collection chamber for collecting concentrated sample or filtrate, which centrifugal filter, when subjected to a driving force, such as centrifugation, passes the filter into a collection tube, thereby obtaining filtrate and concentrate.

However, the filter has a small volume due to factors such as a use scene, and in order to meet the requirements of high sealing performance and high permeability of a filter channel, a plurality of parts are required to be precisely matched and installed to meet the use requirement, so that the production and processing difficulty is high, and the product cost is high. A centrifugal filter which is simple in structure and can realize centrifugal filtration is needed.

SUMMERY OF THE UTILITY MODEL

The utility model provides a centrifugal filter device, simple structure has reduced the production degree of difficulty and processing cost.

In one embodiment, the present invention provides a centrifugal filter apparatus comprising a filter and a collection tube;

the filter comprises a filtering base body and a filtering membrane, the filtering base body comprises a sample inlet opening arranged at a first end of the filtering base body, a filtering cavity communicated with the sample inlet opening, and a flow guide hole arranged on the filtering base body and at a second end corresponding to the first end, and a dead volume chamber is arranged at the position, close to the second end, of the filtering cavity; the filter membrane is arranged at the second end of the filter base body, an included angle is formed between the filter membrane and the axis of the filter base body, and the included angle is larger than 0 degree and smaller than 90 degrees;

the collecting tube has a collecting chamber and a collecting opening, the filter base body being detachably connected to the collecting tube.

In one embodiment, the filter base comprises a side wall and a bottom wall connected to the side wall, the bottom wall and the side wall enclose to form the filter cavity, and the side wall encloses at a first end to form the sample inlet opening; the bottom wall is provided with a first part and a second part connected with the first part, the first part is provided with flow guide holes, the filter membrane is arranged on the flow guide holes, the second part is not provided with flow guide holes, and the flow guide holes and the side wall enclose to form the dead volume chamber.

In one embodiment, the second portion is recessed away from the first end and encloses a dead volume chamber with the sidewall.

In one embodiment, the second portion extends along an extension of the first portion and encloses with the sidewall a dead volume chamber.

In one embodiment, the included angle is 30-60 °.

In one embodiment, the first end of the side wall is provided with a limiting part, the limiting part protrudes out of the outer surface of the side wall and is arranged around the side wall, and the limiting part is used for being clamped and connected with the collection opening of the collection pipe.

In one embodiment, the limiting part is an annular protruding structure, and the protruding structure is used for clamping the filter on the end face of the collecting opening of the collecting pipe.

In one embodiment, the limiting part comprises a plurality of protruding structures, the protruding structures are arranged along the circumferential direction of the side wall, and the protruding structures are used for clamping the filter on the collecting opening end face of the collecting pipe.

In one embodiment, the side of the collection opening is hinged to a cover.

In one embodiment, the filter can be detachably clamped with the collecting pipe optionally in a first posture or a second posture, when the filter is detachably clamped with the collecting pipe in the first posture, the flow guide hole extends into the collecting cavity, and sample liquid is filtered by the filter membrane under the centrifugal action and then is discharged into the collecting cavity through the flow guide hole; the filter with when the collecting pipe is with second gesture detachably joint, the access opening stretches into collect the chamber, remains after filtering sample liquid in the dead volume room is through under the centrifugal action access opening gets into collect the intracavity.

According to the centrifugal filter device in the embodiment, the centrifugal filter device comprises a filter and a collecting pipe, the filter is used for filtering sample liquid, the filter comprises a filter base body and a filter membrane, the filter base body comprises a sample inlet opening arranged at a first end of the filter base body, a filter cavity communicated with the sample inlet opening, and a flow guide hole arranged on the filter base body and at a second end corresponding to the first end, and a dead volume chamber is arranged at the position, close to the second end, of the filter cavity; the filter membrane is arranged at the second end of the filter base body, an included angle is formed between the filter membrane and the axis of the filter base body, and the included angle is larger than 0 degree and smaller than 90 degrees. The collecting tube is used for collecting the filtrate or the concentrate, and is provided with a collecting cavity and a collecting opening, and the filter base body is detachably connected with the collecting tube. Because filter membrane and water conservancy diversion hole set up in the same end of filtering the base member, simplified the structure of filter, reduced the precision requirement of each spare part assembly of device, reduced the processing degree of difficulty to improve production efficiency, reduced product manufacturing cost, improved the inseparable laminating degree in filter and collection chamber, can improve the stability of being connected of filter and collecting pipe.

Drawings

FIG. 1 is a schematic view of an embodiment of a centrifugal filter assembly;

FIG. 2 is an exploded view of a centrifugal filter apparatus according to an embodiment;

FIG. 3 is a schematic axial sectional view of a centrifugal filter device according to an embodiment;

FIG. 4 is a schematic axial sectional view of a filter according to an embodiment;

FIG. 5 is a schematic axial sectional view of a collector tube according to one embodiment;

FIG. 6 is an enlarged view of a portion A of FIG. 4;

FIG. 7 is a schematic view showing an axial sectional structure of a filter according to another embodiment;

FIG. 8 is a schematic axial sectional view of a centrifugal filter device according to another embodiment;

wherein: 1. the device comprises a filter, 11, a filter matrix, 111, a sample inlet opening, 112, a filter cavity, 1121, a dead volume chamber, 113, a diversion hole, 114, a side wall, 115, a bottom wall, 1151, a first part, 1152, a second part, 12, a filter membrane, 2, a collecting pipe, 21, a collecting opening, 22, a collecting cavity, 3, a limiting part, 4 and a cover body.

Detailed Description

The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

In addition, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments, and the operation steps involved in the embodiments may be sequentially exchanged or adjusted in a manner that can be obvious to those skilled in the art. Accordingly, the description and drawings are merely for clarity of description of certain embodiments and are not intended to necessarily refer to a required composition and/or order.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" as used herein includes both direct and indirect connections (couplings), unless otherwise specified.

This embodiment provides a centrifugal filter device that can be used to separate biological substances such as abzymes, nucleic acids, and proteins for the purposes of concentration, desalination, purification, and fractionation, such as concentrating urine, serum, plasma, and cerebrospinal fluid.

Referring to fig. 1, the device comprises a filter 1 and a collecting pipe 2, wherein the filter 1 and the collecting pipe 2 at least partially extend into the collecting pipe 2, the filter 1 is used for filtering and centrifuging a sample liquid to be filtered, and the collecting pipe 2 is used for collecting a filtered filtrate or a concentrated liquid (such as concentrated urine).

Referring to fig. 3, the filter 1 includes a filter base 11 and a filter membrane 12, the filter membrane 12 is disposed on the filter base 11, the filter base 11 includes a sample inlet 111 disposed at a first end of the filter base 11, a filter cavity 112 communicated with the sample inlet 111, and a flow guide hole 113 disposed on the filter base 11 and corresponding to a second end of the first end, and a dead volume chamber 1121 is disposed at a position of the filter cavity 112 near the second end; the sample liquid can enter the filter cavity 112 from the sample inlet 111 and is filtered by the filter membrane 12 to form a filtered liquid, the filtered liquid can be discharged from the flow guide hole 113 out of the filter cavity 112, and an unfiltered sample liquid (hereinafter, described as a concentrated liquid) remains in the dead volume 1121.

Further, the filtering base 11 is a closed shell structure with one end open, and is preferably a hollow cylinder.

Furthermore, the flow guide holes 113 are provided in a plurality, the flow guide holes 113 are distributed in an array manner, the flow guide holes 113 are in a cylindrical or conical structure or a horn-shaped structure, and the axis of the flow guide holes is parallel to the axis of the filter substrate 11, so that the filtrate is guided into the collection pipe 2. Of course, in other embodiments, the axis of the diversion hole 113 may be perpendicular to its centerline.

Referring to fig. 2 and 5, the collecting tube 2 has a collecting cavity 22 and a collecting opening 21, when in use, the filter 1 extends into the collecting cavity 22 from the collecting opening 21, when sample liquid is separated and filtered, the diversion hole 113 of the filter 1 extends into the collecting cavity 22, after the sample liquid is filtered by the filter membrane 12, filtrate can be drained from the diversion hole 113 to the collecting cavity 22, the concentrated liquid is collected in the dead volume chamber 1121, the filter 1 is detached from the collecting tube 2, and the concentrated liquid in the dead volume chamber 1121 is transferred to other experimental containers (such as test tubes) for storage, so as to achieve different experimental purposes.

Referring to fig. 4 and 6, in an embodiment, the filter base 11 includes a side wall 114 and a bottom wall 115 connected to the side wall 114, the bottom wall 115 and the side wall 114 enclose to form the filter cavity 112, and the side wall 114 encloses at a first end to form the sample inlet opening 111; the bottom wall 115 has a first portion 1151 and a second portion 1152 connected to the first portion 1151, the first portion 1151 is provided with a diversion hole 113, the filter membrane 12 is disposed on the diversion hole 113 for guiding the filtered sample liquid to the collection cavity 22, and the second portion 1152 is not provided with a diversion hole 113, which encloses the side wall 114 to form the dead volume chamber 1121.

Referring to fig. 3, in the embodiment, the second portion 1152 is recessed away from the first end, and encloses with the sidewall 114 to form a dead volume chamber 1121, the dead volume chamber 1121 may be a regular three-dimensional structure or an irregular three-dimensional structure protruding from the filtering base 11, and when the dead volume chamber is a regular three-dimensional structure, the axial cross section of the dead volume chamber may be a rectangle, a triangle, or a trapezoid.

Referring to fig. 7 and 8, in another embodiment, the second portion 1152 extends along an extension of the first portion 1151 and encloses the sidewall 114 to form a dead volume chamber 1121, or the first portion 1151 extends directly to the sidewall 114 and encloses the sidewall 114 to form the dead volume chamber 1121, and the dead volume chamber 1121 has a substantially triangular structure in axial cross section.

In order to reduce the processing difficulty of the filter 1, improve the processing efficiency, and achieve the reduction of the production cost of the product, different from the existing centrifugal filter, this embodiment simplifies the structure of the filter 1, and the dead volume chamber 1121, the filter membrane 12, and the diversion hole 113 are all located at the second end of the filter matrix 11, that is, the filter membrane 12 and the dead volume chamber 1121 are disposed at the same end of the filter matrix 2, so as to achieve the separation of the filtrate and the concentrated solution. The sample liquid enters the filter cavity 112 through the sample inlet 111, and is filtered through the filter membrane 12 positioned higher than the dead volume chamber 1121, the filtered filtrate is drained from the flow guide hole 113 to the collection cavity 22 for storage, and the target substance of the experiment is concentrated and gathered into a concentrated solution which is collected in the dead volume chamber 1121 for the subsequent experiment to be moved. Of course, when the filter 1 and the collection pipe 2 are installed in the embodiment, the side wall 114 can be closely attached to the inner wall of the collection chamber 22, and the connection stability of the collection pipe 2 and the filter 1 can be improved.

Further, an included angle α is formed between the axis of the filter membrane 12 and the axis of the filter cavity 112, as shown in fig. 4, and is a smaller included angle between the top of the filter membrane 12 and the axis, for example, when the filter membrane 12 is disposed on the left side of the dead volume chamber 1121, the included angle (acute angle) is formed between the left side of the filter membrane 12 and the axis, when the filter membrane 12 is disposed on the right side of the dead volume chamber 1121, the included angle α is greater than 0 ° and less than 90 °, due to the inclined disposition of the filter membrane 12, the dead volume chamber 1121 is disposed on the lowest end side of the filter membrane 12, an axial height difference exists between the filter membrane 12 and the dead volume chamber 1121, after the sample liquid is filtered by the filter membrane 12, the sample liquid flows from the diversion holes 113 to the collection cavity 22 by gravity, and the concentrated liquid flows from the inclined plane of the filter membrane 12 to the dead volume chamber 1121 which is disposed at a position lower than the filter membrane 12 and collects.

Further, in order to realize better filtering separation effect, the included angle alpha is 30-60 degrees. Preferably, the included angle α is 60 °.

In order to realize different experimental separation purposes and facilitate the collection of concentrated solution, the filter 1 can be detachably connected with the collecting pipe 2 in a first posture (forward installation) or a second posture (reverse installation), and when the filter 1 is detachably clamped with the collecting pipe 2 in the first posture, the guide hole 113 extends into the collecting cavity 22, so that the filtrate in the filter cavity 112 can be discharged into the collecting cavity 22 through the guide hole 113; filter 1 with when collecting pipe 2 with second posture detachably joint, sampling opening 111 stretches into collect chamber 22 to make the concentrate in dead volume room 1121 can follow sampling opening 111 discharges to in collecting chamber 21, made things convenient for the separation and the independent collection of the concentrate of filtrating, pollution and the waste brought when having avoided the pipettor to move and get the concentrate.

In order to avoid filter 1 falling into collecting pipe 2, the first end of lateral wall 114 is equipped with spacing portion 3, spacing portion 3 protrusion in the surface of lateral wall 114, and set up in lateral wall 114 is all around, spacing portion 3 be used for with collecting opening 21 block connection of collecting pipe 2. When filter 1 is connected with collecting pipe 2 with first gesture, 2 card of spacing portion are established on collecting opening 21 terminal surfaces of collecting pipe 3, can avoid filter 1 to fall into collecting pipe 3, have solved the filter 1 that leads to from this and have taken difficultly, the extravagant and contaminated problem of filtrating.

In this embodiment, referring to fig. 2, the limiting portion 3 is an annular protruding structure, the protruding structure is used to connect the filter 1 to the end surface of the collection opening 21 of the collection pipe 2, the end surface of the protruding structure abuts against the end surface of the collection opening 21 to limit the filter 1 from further extending into the collection pipe 2, and the protruding structure may be disposed between the diversion hole 113 and the first end of the filter substrate 11, i.e., disposed above the diversion hole 113 in this embodiment.

In another embodiment, the position-limiting portion 3 includes a plurality of protruding structures, the protruding structures are arranged along the circumferential direction of the side wall 114, and the protruding structures are used for clamping the filter 1 on the end surface of the collection opening 21 of the collection pipe 2.

In this embodiment, the side of the collecting opening 21 is hinged with the cover body 4, and after the filtrate or the concentrated solution enters the collecting pipe 3, the filtrate or the concentrated solution is stored in a sealed manner by the cover body 4, so that waste and pollution are avoided. Of course, in other embodiments, the collection opening 21 may be provided with an external thread, and be connected with a cover body provided with an internal thread, or the collection opening 21 may be provided with a plug.

In the centrifugal filter device in this embodiment, the filter membrane 12 and the dead volume 1121 of the filter 1 are both disposed at the second end, the dead volume 1121 is disposed at one side of the filter membrane 12, an included angle is formed between the axis of the filter membrane 12 and the axis of the filter substrate 11, when filtering and separating, the filter 1 is first connected to the collecting pipe 2 in a first posture, the limiting portion 3 is clamped on the end surface of the collecting opening 21 of the collecting pipe 2, the filter 1 can be prevented from dropping into the collecting pipe 2, a sample liquid to be filtered enters the filter cavity 112 from the sample inlet 111, is filtered by the filter membrane 12, and passes through the flow guide hole 113 and the collecting pipe 3, and a concentrated solution (such as a target substance which is not filtered or is filtered) is collected in the dead volume 1121 due to the existence of a height difference under the action of gravity, so that the filtering and separating of the sample liquid are achieved, when the filter 1 is mounted with the collecting pipe 2 in a second posture, the concentrated solution is guided to the collecting cavity 22 from the sample inlet 111, so that the purpose of different filtering and separating experiments (experiments of filtrates and concentrated solutions or experiments of different concentrated solutions) is achieved.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical personnel in the technical field of the utility model, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replace.

Claims

1. The centrifugal filtering device is characterized by comprising a filter and a collecting pipe;

2. The centrifugal filter device of claim 1, wherein the filter base includes a side wall and a bottom wall connected to the side wall, the bottom wall enclosing the side wall to form the filter cavity, the side wall enclosing the sample inlet opening at a first end; the bottom wall is provided with a first part and a second part connected with the first part, the first part is provided with a flow guide hole, the filter membrane is arranged on the flow guide hole, the second part is not provided with a flow guide hole, and the flow guide hole and the side wall enclose to form the dead volume chamber.

3. A centrifugal filter device according to claim 2 wherein the second portion is recessed away from the first end and encloses a dead volume chamber with the side wall.

4. A centrifugal filter device according to claim 2 wherein the second portion extends along the extension of the first portion and encloses a dead volume chamber with the side wall.

5. A centrifugal filter device according to claim 1 wherein the included angle is 30-60 °.

6. The centrifugal filter device of claim 2, wherein the first end of the sidewall is provided with a limiting portion protruding from the outer surface of the sidewall and disposed around the sidewall, the limiting portion being adapted to engage with the collection opening of the collection tube.

7. The centrifugal filter apparatus of claim 6 wherein said stop is an annular projection configured to snap the filter onto the collection opening end of said collection tube.

8. The centrifugal filter apparatus of claim 6, wherein said limiting portion comprises a plurality of raised structures arranged along a circumference of said sidewall, said raised structures being configured to retain said filter on said collection opening end surface of said collection tube.

9. A centrifugal filter device as claimed in claim 1, wherein the sides of the collection opening are hinged to a cover.

10. The centrifugal filter apparatus according to any one of claims 1 to 9 wherein said filter is removably engageable with said collection tube, optionally in a first position or a second position, and when said filter is removably engageable with said collection tube in said first position, said baffle hole extends into said collection chamber, and sample liquid is centrifuged through said filter membrane and discharged through said baffle hole into said collection chamber; the filter with when the collecting pipe was with second posture detachably joint, the access opening stretches into collect the chamber, remain after filtering sample liquid in the dead volume chamber is through under the centrifugal action the access opening gets into collect the intracavity.