CN217931573U - Liquid equipartition detection unit suitable for direct centrifugal microfluidic chip - Google Patents
Liquid equipartition detection unit suitable for direct centrifugal microfluidic chip Download PDFInfo
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- CN217931573U CN217931573U CN202221431437.7U CN202221431437U CN217931573U CN 217931573 U CN217931573 U CN 217931573U CN 202221431437 U CN202221431437 U CN 202221431437U CN 217931573 U CN217931573 U CN 217931573U
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- 239000007788 liquid Substances 0.000 title claims abstract description 84
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 238000006243 chemical reaction Methods 0.000 claims abstract description 40
- 239000000758 substrate Substances 0.000 claims abstract description 34
- 238000003860 storage Methods 0.000 claims abstract description 13
- 230000007704 transition Effects 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 8
- 238000005452 bending Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000012935 Averaging Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000009827 uniform distribution Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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Abstract
The utility model discloses a liquid equipartition detection unit suitable for a direct centrifugal microfluidic chip, which comprises a liquid equipartition detection unit arranged on a circular substrate; the liquid equipartition detection unit comprises a gas storage chamber, a connecting channel, a quantitative pool, a capillary channel and a reaction pool; the quantitative pool and the reaction pool are arranged at intervals along the radial direction towards the direction far away from the circle center of the circular substrate and are communicated through the capillary channel; step valves or transition holes are arranged at any positions of the capillary channels; the gas storage tank is arranged on the side wall of the quantitative tank close to the circle center of the circular substrate; the two connecting channels are respectively positioned at two sides of the gas storage pool and communicated with the quantitative pool. The utility model discloses aim at solving that current centrifugal micro-fluidic chip's circular substrate structure is complicated, centrifugal operation needs multistage rotational speed, joins in marriage the inaccurate problem of liquid.
Description
Technical Field
The utility model relates to a little full analytic system especially relates to and is suitable for direct centrifugal micro-fluidic chip's liquid to equally divide detecting element.
Background
With the development of Micro Total Analysis Systems (μ -TAS), centrifugal microfluidic chips have been widely used in the detection fields of biology, chemistry, medicine, environment, food, etc. to provide a carrier for the reaction and detection of the sample to be detected and the reagent, with the advantages of high throughput, high integration, high automation, convenient carrying and low processing cost.
At present, the scheme of equally dividing liquid by adopting a centrifugal microfluidic analysis chip mainly comprises two schemes: one is directly carrying out a centrifugal uniform division method, and the other is carrying out a centrifugal uniform division method after primary uniform division.
The direct centrifugal uniform dividing method is that the liquid to be measured is added into the wave-shaped channel, and then the centrifugal operation is carried out, so that the liquid is uniformly divided into the reaction tanks through the capillary passive valves to be reacted. For example, the chinese patent publication No. CN103831140B discloses a "multi-index detection microfluidic chip", the liquid equipartition detection unit of the microfluidic chip consists of a wave-shaped main channel, a buffer pool and a reaction pool; when the device works, liquid is added into the wavy main channel, the liquid is forced into the reaction holes through centrifugal driving, and the buffer liquid pool is adopted to supplement the liquid to the reaction pool. Although the micro-fluidic chip realizes the uniform distribution operation of the liquid in the reaction tank, the reaction tank and the buffer tank are of an integral structure which is communicated, and the volume of the liquid which actually participates in the reaction is the sum of the volumes of the liquid in the buffer tank and the liquid in the reaction tank, so that errors still exist in the detection result of the structure. And when the centrifugal rotation is carried out, in the process that gas in the reaction tank is replaced into the wave-shaped main channel, a part of liquid in the wave crest of the wave-shaped main channel can be pushed into the adjacent channel, so that the liquid distribution in the liquid separating tank is inaccurate, and the uniformity of uniformly distributing the liquid in the buffer tank and the reaction tank cannot be ensured.
Disclosure of Invention
An object of the utility model is to provide a detecting element is equallyd divide to liquid that is suitable for direct centrifugal micro-fluidic chip realizes improving the material cost and the processing cost who divide equally the precision, reduce circular substrate that detect liquid.
In order to achieve the above purpose, the utility model can adopt the following technical proposal:
the liquid equipartition detection unit suitable for the direct centrifugal microfluidic chip comprises a liquid equipartition detection unit arranged on a circular substrate; the liquid equipartition detection unit comprises a gas storage chamber, a connecting channel, a quantitative pool, a capillary channel and a reaction pool; the quantitative pool and the reaction pool are arranged at intervals along the radial direction towards the direction far away from the circle center of the circular substrate and are communicated through the capillary channel; step valves or transition holes are arranged at any positions of the capillary channels; the gas storage tank is arranged on the side wall of the quantitative tank close to the circle center of the circular substrate; the two connecting channels are respectively positioned at two sides of the gas storage pool and communicated with the quantitative pool.
The utility model discloses aim at solving that current centrifugal micro-fluidic chip's circular substrate structure is complicated, centrifugal operation needs multistage rotational speed, joins in marriage the inaccurate problem of liquid.
Optionally, the volume of the quantitative pool is 0.8-1.2 times of the volume of the reaction pool, and the quantitative pool is used for realizing primary equalization and quantification of liquid volume.
It is found from many experiments that when the ratio of the longitudinal length to the transverse length of the dosing chamber is less than or equal to 1.2: 1, the dosing chamber is more easily filled with liquid.
Optionally, a step valve or a transition hole is arranged at any position of the capillary channel; the step valve or the transition hole is used for improving the reliability of the microfluidic analysis chip, reducing the length of a capillary channel, reducing the diameter size of the circular substrate and simultaneously avoiding the backflow of liquid.
Optionally, the radial included angle between the axis of the capillary channel and the round substrate is less than or equal to 70 degrees, so that the size of the microfluidic analysis chip can be reduced, and the cost can be reduced; of course, the capillary channel may also be curved or otherwise linear.
Optionally, two connecting channels in the liquid equal-distribution detection unit are respectively arranged in an extending manner towards the center of the circle of the circular substrate or away from the center of the circle of the circular substrate; when the two connecting channels in the liquid equipartition detection unit extend towards the circle center of the circular substrate, under the action of centrifugal force, liquid in the connecting channels can respectively flow into the quantitative tanks respectively connected with the connecting channels, so that liquid distribution inaccuracy caused by migration of the liquid in the quantitative tanks into the connecting channels is avoided; when two connecting channels in the liquid equal-dividing detection unit extend towards the direction away from the circle center, residual liquid in the bent section of the connecting channel separates two adjacent liquid equal-dividing detection units, and therefore, the situation that volatile gas enters the two adjacent liquid equal-dividing detection units during reaction to cause inaccurate detection results is avoided.
The utility model discloses set up the gas receiver in the ration pond, avoided in the reaction tank gas of displacement enter into interface channel, cause the interior liquid migration of interface channel to adjacent reaction tank, cause and join in marriage the liquid inaccuracy. Meanwhile, as the sectional dimension of the connecting channel is far smaller than that of the quantitative pool, the fluid resistance of the liquid flowing to the connecting channel in the rotary centrifugal process is increased, and the accuracy of liquid preparation is improved. The utility model discloses do not use siphon passageway and wave passageway, simple structure, the processing degree of difficulty is low, and has reduced supporting instrument cost.
Drawings
Fig. 1 is a schematic structural view of the liquid equal-dividing detecting unit of the present invention.
Fig. 2 is another schematic structural view of the liquid equal-dividing detecting unit of the present invention.
Fig. 3 is a schematic structural view of the step valve in fig. 1 modified with a transition hole.
Fig. 4 is a schematic view showing a structure in which the capillary passage of fig. 1 is obliquely arranged.
Fig. 5 is a schematic view of a top-view structure in which a plurality of liquid-averaging detecting units are arranged on the circular substrate.
Fig. 6 is a schematic bottom view of the structure of fig. 5.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that all the directional indicators (such as up, down, left, right, front, back \8230;) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
In the present application, unless expressly stated or limited otherwise, the terms "connected" and "secured" are to be construed broadly, and thus, for example, "secured" may be a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In addition, the meaning of "and/or" appearing throughout is to include three juxtapositions, exemplified by "A and/or B," including either the A or B arrangement, or both A and B satisfied arrangement. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
As shown in fig. 1, the liquid equal-dividing detecting unit of the present invention comprises an air storage chamber 1, a connecting channel 2, a quantifying pool 3, a capillary channel 4, a step valve 5 and a reaction pool 6; the quantitative pool 3 and the reaction pool 6 are arranged at intervals along the radial direction towards the direction far away from the circle center of the circular substrate and are communicated with each other through a capillary channel 4; the step valve 5 is positioned at any position of the capillary channel 4 and is used for improving the reliability of the microfluidic analysis chip, reducing the length of the capillary channel 4 and reducing the diameter size of the circular substrate; the gas storage tank 1 is arranged on the side wall of the quantitative tank 3 close to the circle center of the circular substrate and is of a side-opening concave cavity structure; the connecting channels 2 are two extending channels towards the circle center of the circular substrate, are respectively positioned at two sides of the gas storage tank 1 and are communicated with the quantitative tank 3.
Beneficially or exemplarily, as an embodiment, as shown in fig. 2, two connecting channels 2 are extended and disposed away from the center of the circular substrate, and a concave curved segment 7 is disposed on each of the two connecting channels 2, so that after the chip body is rotated and centrifuged, two adjacent liquid equal-division detecting units are separated by the liquid sealed in the connecting channels 2, so as to prevent the volatile gas from entering the two adjacent liquid equal-division detecting units during the reaction, thereby ensuring the accuracy of the detection result.
Beneficially or exemplarily, as an embodiment, as shown in fig. 3, the step valve 5 is changed into the transition hole 8, on one hand, gas-liquid exchange is facilitated, the sample injection pressure in the reaction tank 6 is reduced, and the rotation speed can be reduced; on the other hand, due to the height difference, bubbles generated at the bottoms of the transition holes 8 are uniformly distributed, and in the reaction process, the volume of the bubbles is gradually increased to isolate the capillary channel 4 from the reaction tank 6, so that the reliability of the sample detection result is guaranteed.
Beneficially or exemplarily, as an embodiment, as shown in fig. 4, the radial included angle α between the axis of the capillary channel 4 and the circular substrate 1 is less than or equal to 70 °, so that the size of the microfluidic analysis chip can be reduced, and the cost can be reduced; of course, the capillary channel 4 may also be curved or otherwise linear.
Advantageously or exemplarily, as an embodiment, the volume of the quantitative pool 3 is 0.8 to 1.2 times the volume of the reaction pool 6, so as to achieve the uniform distribution of the liquid; it is found from many experiments that when the ratio of the longitudinal length to the transverse length of the dosing chamber 3 is less than or equal to 1.2: 1, the dosing chamber is more easily filled with liquid.
Illustratively, as shown in fig. 5 and 6, a plurality of liquid-averaging detecting units are arranged circumferentially on a circular substrate 9. Three positioning holes 9.1 for connecting the rotating shaft are formed in the circular substrate 9; the circular substrate 9 is provided with a sample inlet 9.2, an exhaust hole 9.3 and an exhaust channel 9.4; the sample inlet hole 9.2 can be a cylindrical or conical hole and is used for realizing sample inlet operation of a sample; exhaust hole 9.3 is used for realizing that the atmospheric pressure in the detecting element is equallyd divide to each liquid in the application of sample in-process is invariable.
One end of a set first liquid equipartition detection unit is communicated with a sample inlet hole 9.2 through a sample inlet channel 9.5, and one connecting channel 2 at the other end is communicated with one connecting channel of an adjacent liquid equipartition detection unit; one connecting channel of the last liquid equipartition detection unit is communicated with an exhaust hole 9.3 through an exhaust channel 9.4, and the other connecting channel 2 is communicated with one connecting channel 2 of the adjacent liquid equipartition detection unit; and the rest liquid equipartition detection units are respectively communicated with the connecting channels 2 of the adjacent liquid equipartition detection units through the connecting channels 2.
During manufacturing, the reaction tank 6 is a through hole structure penetrating through the substrate 9, and the capillary channels 4 are all through groove structures penetrating through the substrate 9; the sample inlet hole 9.2, the exhaust hole 9.3, the exhaust channel 9.4, the quantitative pool 3 and the connecting channel 2 are arranged on the front surface of the substrate 9; the sample introduction channel 9.5 is arranged on the reverse side of the substrate 9; and bonding cover plates on the upper surface and the lower surface of the circular base plate 9 to manufacture the centrifugal microfluidic chip.
When the centrifugal microfluidic chip works, a 5ml pipette is used, a certain amount of sample is added into the quantitative pool 3 from the sample inlet hole 9.2 through the sample inlet channel 9.5, and liquid flows along the longitudinal and transverse side walls of the quantitative pool 3 under the action of tension in the process of flowing into the quantitative pool 3; when the liquid flows to the inlet of the next connecting channel 2 along the transverse side wall, the flowing of the liquid is subjected to resistance because of the sudden change of the wall surface structure at the joint of the transverse side surface of the quantitative pool 3 and the connecting channel 2, the liquid can continuously flow for a certain distance along the longitudinal wall surface of the quantitative pool 3 and then contacts the rear side wall surface of the quantitative pool 3, and under the action of tension, the liquid can quickly flow through the rear side wall surface and fill the quantitative pool 3.
Placing the centrifugal microfluidic chip added with the sample on a centrifugal detection platform, and rotating counterclockwise for 3s at the rotating speed of 800 r/min; the sectional area of the quantitative pool 3 is far larger than that of the connecting channel 2, so that the flow resistance of the liquid in the connecting channel 2 is increased, and the stability of the volume of the liquid in each adjacent quantitative pool is guaranteed.
The liquid in the quantitative pool 3 breaks through the step valve 5 and the capillary channel 4 in sequence under the driving of centrifugal force, and enters the reaction pool 6 coated with the reaction medicament for reaction; after liquid enters the reaction tank 6, gas in the reaction tank 6 is compressed instantly, and when the gas pressure in the reaction tank 6 is greater than the centrifugal pressure of the liquid, the gas in the reaction tank 6 is extruded to move in the reverse direction of the centrifugal direction, and then is displaced into the quantitative tank 3 and is collected in the gas storage chamber 1, so that the liquid in the connecting channel is prevented from flowing to the adjacent quantitative tank due to the fact that the gas enters the connecting channel 2, and the quantitative equal-division operation of the liquid is realized.
After the reaction is finished, the reaction results in the reaction tank are sequentially detected through related detection equipment, and the detection results are output.
The utility model discloses a noun explains:
the step valve is a valve structure which has no moving part and makes the size of the flowing position of fluid suddenly change in a micro-channel through a step structure, thereby realizing the retardation of a contact angle at a geometrical singular point and stopping capillary flow.
The capillary channel is a pipe with a small diameter and a small length, and when liquid passes through the capillary channel, pressure drop is generated along the direction of the capillary channel, so that the flow of the liquid is blocked.
Claims (6)
1. A liquid equipartition detection unit suitable for a direct centrifugal microfluidic chip comprises a liquid equipartition detection unit arranged on a circular substrate; the method is characterized in that:
the liquid equipartition detection unit comprises an air storage chamber, a connecting channel, a quantitative pool, a capillary channel and a reaction pool; the quantitative pool and the reaction pool are arranged at intervals along the radial direction towards the direction far away from the circle center of the circular substrate and are communicated through the capillary channel; the gas storage tank is arranged on the side wall of the quantitative tank close to the circle center of the circular substrate; the two connecting channels are respectively positioned at two sides of the gas storage pool and communicated with the quantitative pool.
2. The liquid equipartition detection unit for a direct centrifugal microfluidic chip according to claim 1, wherein: the ratio of the longitudinal length to the transverse length of the quantitative pool is less than or equal to 1.2: 1.
3. The liquid equipartition detection unit suitable for the direct centrifugal microfluidic chip according to claim 1 or 2, wherein: the volume of the quantitative pool is 0.8-1.2 times of the volume of the reaction pool.
4. The liquid equipartition detecting unit for a direct centrifugal microfluidic chip according to claim 1 or 2, wherein: step valves or transition holes are arranged at any positions of the capillary channels.
5. The liquid equipartition detection unit suitable for the direct centrifugal microfluidic chip according to claim 1 or 2, wherein: the radial included angle between the axis of the capillary channel and the round substrate is less than or equal to 70 degrees.
6. The liquid equipartition detection unit suitable for the direct centrifugal microfluidic chip according to claim 1 or 2, wherein: the liquid is equally divided into two connecting channels in the detection unit, the two connecting channels are respectively arranged towards the circle center direction of the circular substrate or away from the circle center direction of the circular substrate, and a bending section is arranged on the two connecting channels.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221431437.7U CN217931573U (en) | 2022-06-09 | 2022-06-09 | Liquid equipartition detection unit suitable for direct centrifugal microfluidic chip |
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| CN202221431437.7U CN217931573U (en) | 2022-06-09 | 2022-06-09 | Liquid equipartition detection unit suitable for direct centrifugal microfluidic chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023236787A1 (en) * | 2022-06-09 | 2023-12-14 | 安图实验仪器(郑州)有限公司 | Centrifugal microfluidic analysis chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023236787A1 (en) * | 2022-06-09 | 2023-12-14 | 安图实验仪器(郑州)有限公司 | Centrifugal microfluidic analysis chip |
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