CN217600715U - A device for the enrichment growth and controllable harvest of Chlorella induced by interfacial carbon dioxide - Google Patents

Abstract

The utility model belongs to the technical field of little algae is cultivateed and is retrieved, a device that interfacial carbon dioxide induced chlorella enrichment growth and controllable gathering is disclosed. The device comprises a photosynthetic reactor, a liquid inlet tank, a gas supply device and an illumination incubator, wherein the photosynthetic reactor is arranged in the illumination incubator, and a light source and a heating device are arranged in the illumination incubator; the photosynthetic reactor comprises a transparent shell and a porous hydrophobic medium filled in the transparent shell, wherein the transparent shell is provided with an air inlet communicated with a gas supply device, a liquid inlet communicated with a liquid inlet groove and a liquid outlet, and the porous hydrophobic medium is filled in a transparent shell between the liquid inlet and the liquid outlet; the porous hydrophobic medium comprises at least one of a hollow fiber hydrophobic gas film, a flat plate type hydrophobic gas film and a hydrophobic porous silicone tube. The device can freely switch the chlorella enrichment growth and controllable harvesting mode, has reliable integral structure, simple operation and stable operation, and has good market application prospect.

Description

A device for the enrichment growth and controllable harvest of Chlorella induced by interfacial carbon dioxide

technical field

The utility model belongs to the technical field of microalgae cultivation and recovery, in particular to a device for inducing enrichment growth and controllable harvesting of chlorella by interface carbon dioxide.

Background technique

At present, CO ₂ storage and capture technologies mainly include physical adsorption, chemical absorption and biological capture. Among them, physical adsorption method and chemical absorption method are two widely used methods, but these two methods have relatively high cost and energy consumption, and require temperature and pressure swing operation or dosing of chemicals to achieve CO ₂ recovery and storage.

Bio-capture means that microorganisms such as microalgae use CO ₂ as the only carbon source during the growth process, so as to achieve CO ₂ capture and fixation. Algae can use _CO2 to grow rapidly and synthesize organic matter, and the generated biomass can be used to prepare chemical raw materials, which has important potential application value for _CO2 emission reduction and energy crisis solution. In addition, the culture medium required for algae growth can use domestic sewage, aquaculture wastewater, source separation urine, etc., to provide necessary nutrients such as nitrogen and phosphorus, and trace elements such as iron and magnesium for the growth of algae, and can also simultaneously achieve high-efficiency treatment of sewage.

Chlorella has a simple cell structure and is only composed of lipids, proteins and hydrocarbons. It is the algal species with the highest growth rate among microalgae, and uses a fast rate of photosynthesis. At present, suspension culture is one of the most widely studied and applied technical means for Chlorella to fix CO ₂ and treat wastewater. The reactors used in it can usually be divided into two types: open ponds and closed-loop cylindrical reactors. However, there are some problems in the suspension culture mode, such as the low concentration of chlorella cultured biomass, and the small shape of chlorella, which is difficult to separate and harvest. The current technology research and development has not simultaneously realized the enrichment growth and controllable harvest of microalgae, and the process also needs to set up high energy consumption units such as aeration, which reduces the economy of the overall system.

Utility model content

In view of the above problems existing in the prior art, the purpose of this utility model is to provide a device for the enrichment growth and controllable harvesting of chlorella induced by interface carbon dioxide, which can freely switch between the enrichment growth and controllable harvesting of chlorella Harvesting mode, the overall structure is reliable, the operation is simple, and the operation is stable.

For realizing the above-mentioned purpose, the technical scheme that the utility model adopts is:

A device for inducing enrichment growth and controllable harvesting of Chlorella by interface carbon dioxide, the device comprises a photosynthesis reactor, a liquid inlet tank, a gas supply device, and a lighting incubator, wherein the photosynthesis reactor is arranged in the lighting incubator , a light source and a heating device are arranged in the illumination incubator;

The photosynthesis reactor includes a transparent shell, a porous hydrophobic medium filled in the transparent shell, and the transparent shell is provided with an air inlet communicated with the gas supply device, and a liquid inlet and a liquid outlet communicated with the liquid inlet tank. The porous hydrophobic medium is filled in the transparent shell between the liquid inlet and the liquid outlet;

The porous hydrophobic medium includes at least one of a hollow fiber hydrophobic gas membrane, a flat hydrophobic gas membrane, and a hydrophobic porous silica gel tube.

Further, the pore size of the porous hydrophobic medium is 20-800 nm; the filling density of the hollow fiber hydrophobic air membrane is 1-5 pieces/10 mL; the filling density of the flat-plate hydrophobic air membrane is 1-5 cm ² /10 mL . Preferably, the hollow fiber hydrophobic gas membrane is filled in the photosynthesis reactor in a bundled form; the flat-plate hydrophobic gas membrane is filled in the photosynthesis reactor in a sheet-like form. Preferably, an optical fiber is arranged between the layers of the flat hydrophobic gas film as an auxiliary light source.

Further, the gas supply device includes a gas storage cylinder and an air inlet pipe connecting the gas storage cylinder and the air inlet of the photosynthesis reactor. It is further preferred that the air intake pipeline is a pressure resistant pipeline. Further preferably, the intake gas flow meter and the intake pressure gauge on the intake pipe are preferred.

Further, an air outlet and an air outlet pipe connected to the air outlet are also provided on the transparent casing, and an air outlet gas flow meter and an air outlet pressure gauge are arranged on the air outlet pipe.

Further, the liquid inlet tank is communicated with the liquid inlet through a liquid inlet pipe, and a peristaltic pump is arranged on the liquid inlet pipe.

Further, the transparent casing is made of high light-transmitting acrylic material to ensure that light can effectively enter the photosynthesis reactor.

Further, the device also includes a clean water tank communicated with the liquid outlet.

Compared with the prior art, the beneficial effects of the present utility model are:

The device of the utility model can freely switch the Chlorella enrichment growth mode and the controllable harvest mode, the overall structure of the device is reliable, the operation is simple, and the operation is stable, and the enrichment, concentration and separation processes can be realized without multi-step or multi-unit, which greatly saves money. The cost of chlorella cultivation and harvesting has a good market application prospect.

The device of the utility model uses a porous hydrophobic medium to construct an interface that can continuously dissolve CO ₂ , provides an inductive force for the enrichment and growth of Chlorella on the interface, and forms a thick Chlorella biofilm on the surface of the porous hydrophobic medium; The force is simple and controllable. By increasing the CO ₂ escape pressure, the chlorella biofilm can be stripped, and the controllable harvest of high-density chlorella biomass is completed.

Description of drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and are used to explain the present invention together with the embodiments of the present invention, and do not constitute a limitation to the present invention. In the attached image:

1 is a schematic diagram of the device structure of Embodiment 1;

FIG. 2 is a schematic diagram of the device structure of Embodiment 2. FIG.

Detailed ways

In order to facilitate understanding of the present utility model, the present utility model will be described more comprehensively and in detail below with reference to the accompanying drawings and preferred embodiments of the specification, but the protection scope of the present utility model is not limited to the following specific embodiments.

Unless otherwise defined, all technical terms used hereinafter have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the protection scope of the present invention.

Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or can be prepared by existing methods.

Example 1

Referring to FIG. 1 , the present embodiment discloses a device for the enrichment growth and controllable harvest of chlorella induced by interface carbon dioxide, including a photosynthesis reactor 1, a liquid inlet tank 7, a gas supply device, an illumination incubator 17, and a clean water tank 11. The photosynthesis reactor 1 is arranged in the illumination incubator 17, and the illumination incubator 17 is provided with a light source (not shown) and a heating device (not shown) for providing the photosynthesis reactor 1 with a light source and a heat source.

In this embodiment, the photosynthesis reactor 1 includes a transparent casing 2 made of high light-transmitting acrylic material, and a porous hydrophobic medium 3 filled in the transparent casing 2. The transparent casing 2 is provided with an air inlet 10 communicating with a gas supply device, and an air inlet 10 connected to the gas supply device. The liquid inlet 5, the air outlet 15 and the liquid outlet 18 communicated with the clean water tank 11 are connected to the liquid tank 7, and the porous hydrophobic medium 3 is filled in the transparent casing 2 between the air inlet 10 and the air outlet 15;

In this embodiment, the gas supply device includes a gas storage cylinder 8, an air inlet pipe connecting the gas storage cylinder 8 and the air inlet 10 of the photosynthesis reactor 1, the air inlet pipe is a pressure-resistant pipe, and the gas outlet is provided with a gas outlet pipe; The inlet gas flow meter 9 and the inlet gas pressure gauge 16 are set on the pipeline, and the outlet gas flow meter 13 and the outlet gas pressure gauge 14 are set on the gas outlet pipeline, which are used to monitor the gas supply amount of CO ₂ and prevent the excessive dissolution of CO ₂ on the growth of microalgae. adversely affect.

In this embodiment, the liquid inlet tank 7 is communicated with the liquid inlet port 5 through a liquid inlet pipe, and a peristaltic pump 6 is arranged on the liquid inlet pipe 6 .

In this embodiment, the porous hydrophobic medium is a hollow fiber hydrophobic gas membrane, which is filled into the photosynthesis reactor in a bundled form; preferably, the hollow fiber hydrophobic gas membrane is a hollow fiber hydrophobic hollow fiber made of polyvinylidene fluoride (PVDF) with an average surface pore size of 200 nm. Air film, packing density is 3 pieces/10 mL.

As one of the preferred solutions of this embodiment, its use example is as follows:

The size of the photosynthesis reactor is Π×4×41cm (ie: Π×radius square×height), and the effective volume is 170mL. The inoculated microalgae adopts Chlorella vulgaris, which was purchased from the Wild Organism Resource Bank of the Chinese Academy of Sciences. , wherein the inoculum of Chlorella accounts for 2/3 of the effective volume of the photosynthesis reactor;

The culture solution is continuously supplemented from the culture solution inlet 5 into the photosynthesis reactor by the water inlet tank 7 through the peristaltic pump 6. The culture solution used is artificial simulated urine diluted 10 times, and the pollutant index parameter is ammonia nitrogen (NH ₃ -N) =239.4 mg/L, nitrate nitrogen (NO ₃ -N) ≈ 0 mg/L, nitrite nitrogen (NO ₂ -N) ≈ 0 mg/L, chemical oxygen demand (COD) = 91.1 mg/L, Total Phosphorus (TP) = 44.4mg/L, pH = 8.1. The hydraulic retention time of the culture solution is 2 days, and the culture solution processed by the photosynthesis reactor is discharged from the culture solution outlet 18 to the clear water tank 11 .

During the enrichment growth stage of microalgae, the gas supply rate of CO ₂ was set to 40 m ³ /m ² /h, and the air pressure was set to 1 kPa. The light source and heating device set in the light incubator ensure that the culture temperature is 25°C, the light intensity is 6000 lux, and the light-dark time ratio is 14h:10h.

The growth cycle of microalgae is 10 days; after stable operation, the chlorella enriched at the interface can reach 81.0% and 85.5% of nitrogen and phosphorus removal in water _; The algal biomass was peeled off from the surface of the porous hydrophobic medium under the influence of air pressure, and the recovery concentration of biomass was 80 g/L, which greatly reduced the harvesting cost of microalgae.

Example 2

Referring to FIG. 2 , the present embodiment discloses a device for the enrichment and controllable harvest of chlorella induced by carbon dioxide at the interface, including a photosynthesis reactor 1 ′, a liquid inlet tank 7 ′, a gas supply device, and an illumination incubator 17 ′ , a clear water tank 11', the photosynthesis reactor 1' is arranged in the illumination incubator 17', and the illumination incubator 17' is provided with a light source (not shown) and a heating device (not shown) for providing the photosynthesis reactor 1. Light source and heat source.

In this embodiment, the photosynthesis reactor 1 ′ includes a transparent casing 2 ′ made of high light-transmitting acrylic material, a porous hydrophobic medium 3 ′ filled in the transparent casing 2 ′, and the transparent casing 2 ′ is provided with an air inlet that communicates with a gas supply device. The port 10', the liquid inlet 5' communicated with the liquid inlet tank 7' and the liquid outlet 18' communicated with the clean water tank 11', the porous hydrophobic medium 3' is filled between the air inlet 10' and the air outlet 15' inside the transparent shell 2';

In this embodiment, the gas supply device includes a gas storage cylinder 8', an air inlet pipe connecting the gas storage cylinder 8' and the air inlet 10' of the photosynthesis reactor 1', the air inlet pipe is a pressure-resistant pipe, and the air inlet pipe is The intake gas flow meter 9' and the intake gas pressure gauge 16' are provided to monitor the CO ₂ gas supply and prevent the excessive dissolution of CO ₂ from adversely affecting the growth of microalgae.

In this embodiment, the liquid inlet tank 7' is communicated with the liquid inlet 5' through the liquid inlet pipe, and a peristaltic pump 6' is provided on the 6' liquid inlet pipe.

In this embodiment, the porous hydrophobic medium is a flat hydrophobic gas membrane, which is filled into the photosynthesis reactor in a lamellar form; preferably, the flat hydrophobic gas membrane is a polyvinylidene fluoride (PVDF) flat hydrophobic membrane with an average surface pore size of 400 nm. Air film, the film packing density is 2 cm ² /10 mL.

As one of the preferred solutions of this embodiment, its use example is as follows:

The size of the photosynthesis reactor is 30×15×15 cm (ie: length×width×height), and the effective volume is 4000 mL. , wherein the inoculum of Chlorella accounts for 2/3 of the effective volume of the photosynthesis reactor;

The culture solution is continuously supplemented from the culture solution inlet 5 into the photosynthesis reactor by the water inlet tank 7 through the peristaltic pump 6, and the culture solution used is domestic sewage, and the pollutant index parameter is ammonia nitrogen (NH ₃ -N) = 44.6 mg/L, Nitrate nitrogen (NO ₃ -N) ≈ 0 mg/L, nitrite nitrogen (NO ₂ -N) = 1.1 mg/L, chemical oxygen demand (COD) = 130.0 mg/L, total phosphorus (TP) = 8.6 mg/L, pH = 8.1. The hydraulic retention time of the sewage is 12 hours, and the culture solution processed by the photosynthesis reactor is discharged from the culture solution outlet 18 to the clear water tank 11 .

During the enrichment growth stage of microalgae, the gas supply rate of CO ₂ was set to 30 m ³ /m ² /h, and the air pressure was set to 1.5 kPa. The light source and heating device set in the light incubator ensure that the culture temperature is 25 °C, the light intensity is 8000 lux, and the light-dark time ratio is 12 h: 12 h.

In the utility model, an optical fiber with a volume rate of 8% is added between the layers of the flat plate type hydrophobic gas film, and the light intensity is 4000 lux, thereby providing an auxiliary light source for the growth of chlorella that is shaded between the flat plates.

The growth cycle of microalgae is 12 days; after stable operation, the chlorella enriched at the interface can remove 87.0% and 92.5% of nitrogen and phosphorus in the water body _; The algal biomass was peeled off the surface of the porous hydrophobic medium under the influence of air pressure, and the recovery concentration of biomass was 90 g/L, which greatly reduced the harvesting cost of microalgae.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the scope of the present invention.

Claims (10)

1. An interfacial carbon dioxide induced chlorella enrichment growth and controllable harvesting device is characterized by comprising a photosynthetic reactor, a liquid inlet tank, a gas supply device and an illumination incubator, wherein the photosynthetic reactor is arranged in the illumination incubator, and a light source and a heating device are arranged in the illumination incubator;

the photosynthetic reactor comprises a transparent shell and a porous hydrophobic medium filled in the transparent shell, wherein the transparent shell is provided with an air inlet communicated with a gas supply device, a liquid inlet communicated with a liquid inlet groove and a liquid outlet, and the porous hydrophobic medium is filled in a transparent shell between the liquid inlet and the liquid outlet;

the porous hydrophobic medium comprises at least one of a hollow fiber hydrophobic gas film, a flat plate type hydrophobic gas film and a hydrophobic porous silicone tube.

2. The device according to claim 1, wherein the porous hydrophobic medium has a pore diameter of 20 to 800 nm; the filling density of the hollow fiber hydrophobic gas film is 1~5/10 mL; the filling density of the flat hydrophobic air film is 1 to 5 cm ² /10 mL。

3. The apparatus of claim 2, wherein the hollow fiber hydrophobic gas membrane is packed in bundle form in a photosynthesis reactor; the flat-plate hydrophobic gas film is filled in the photosynthetic reactor in a lamellar form.

4. The device according to claim 3, wherein optical fibers are arranged between layers of the flat hydrophobic gas film as auxiliary light sources.

5. The apparatus of claim 1, wherein the gas supply device comprises a gas cylinder, and a gas inlet pipe connecting the gas cylinder and the gas inlet of the photosynthesis reactor.

6. The apparatus of claim 5, wherein the inlet conduit is provided with an inlet gas flow meter and an inlet pressure gauge.

7. The device of claim 1, wherein the transparent shell is further provided with an air outlet and an air outlet pipeline connected with the air outlet, and the air outlet pipeline is provided with an air outlet gas flowmeter and an air outlet pressure gauge.

8. The apparatus of claim 1, wherein the liquid inlet tank is communicated with the liquid inlet through a liquid inlet pipeline, and a peristaltic pump is arranged on the liquid inlet pipeline.

9. The device of claim 1, wherein the transparent housing is a high light transmission acrylic material.

10. The apparatus of claim 1, further comprising a clear water tank in communication with the liquid outlet.

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