CN217385520U - Stirring and detection integrated probe - Google Patents

Abstract

The utility model discloses a stirring and detection integrated probe, which comprises a probe and a limit rubber plug, wherein the limit rubber plug is round, the probe is of a cylindrical structure, the limit rubber plug is sleeved on the probe, a flat paddle is fixedly arranged at the lower end of the probe, the surface of the paddle is modified by 3-aminopropyl trimethyl silane, and the paddle is coupled with an antibody or an antigen required by immunodetection; when the upper end of the probe is grabbed and rotated, the limiting rubber plug and the paddle rotate along with the probe simultaneously; the utility model discloses with paddle and probe integration setting, the paddle can stir solution and make its misce bene, and the paddle that the surface is couplet has antibody or antigen also can react with solution as solid phase carrier, has not only simplified the test procedure, and the test result is more accurate moreover.

Description

Stirring and detection integrated probe

Technical Field

The utility model belongs to the field of the external diagnosis of medical instrument, a can realize detecting the solid carrier device that the antibody adsorbs and strengthen stirring ability, concretely relates to stirring and detection integral type probe.

Background

The immunodetection technique is a technique developed to detect a specific antigen using a known antibody or to detect a specific antibody using a known antigen, based on the principle of antigen-antibody specific binding. In the reaction process, the solution to be reacted needs to be stirred first to improve the reaction efficiency and reaction uniformity of the antigen and antibody, the solution is detected by using a solid phase carrier on a probe after being uniformly mixed, and test parameters are read out, wherein the solid phase carrier of the existing probe is generally in a columnar or rod-shaped structure. Reaction efficiency can be improved by sufficient stirring, make the detect reagent possess better detectivity, the method of mixing mainly has shock mixing method and stirring rake mixing method in the experiment at present, the relatively poor rate of utilization of mixing effect that vibrates the mixing method is not high, and stirring rake mixing method is even effectual, nevertheless need stretch into the solution with other stirring rake earlier during the experiment and fully stir the back, stretch into the solid phase carrier in the probe and react in the solution again, not only operate very loaded down with trivial details like this, and can not guarantee the stirring rake cleanliness factor in the experiment, cross contamination appears very easily, influence the experimental degree of accuracy.

Disclosure of Invention

To above-mentioned problem and technical demand, the utility model provides a stirring and detection integral type probe, the stirring rake that will be the mixing solution synthesizes to the solid carrier device of detection antibody adsorptivity in, reaches the simplification test procedure, improves the purpose of experimental accuracy.

The technical scheme of the utility model as follows: a stirring and detection integrated probe comprises a probe and a limit rubber plug, wherein the limit rubber plug is round and is of a cylindrical structure, the limit rubber plug is sleeved on the probe, a flat blade is fixedly arranged at the lower end of the probe, the surface of the blade is modified by 3-aminopropyl trimethyl silane, and an antibody or an antigen required by immunodetection is coupled; when the upper end of probe was snatched the rotation, spacing rubber buffer and paddle were rotatory along with the probe simultaneously. In this scheme, the paddle surface coupling of probe lower extreme detects required antibody or antigen, and the paddle stretches into reaction solution in, can take place to react with solution, reaches the purpose that detects, and the paddle rotation of platykurtic can stir solution simultaneously, can reach fine mixed solution's effect like this, can avoid using the phenomenon that other stirring rod takes place cross infection again.

Further, limiting rubber stopper includes the concentric reaction hole chock plug that sets up, places board and rubber sleeve, and the rubber sleeve cross section is the T type, and its upper end is equipped with annular spacing limit, and rubber sleeve cover is on the probe, places board and reaction hole chock plug and from up the cover outside the rubber sleeve down in proper order, places the diameter of board and is greater than the maximum diameter of reaction hole chock plug, places the board and is restricted between annular spacing limit and reaction hole chock plug. The limiting rubber stopper can protect the probe and limit the length of the probe extending into the solution, and when the probe is placed into a reaction container filled with the solution, the placing plate blocks the opening of the container, so that the blade is immersed into the solution but does not contact the bottom of the container; reaction hole stopper head inlays in the orifice, keeps being enough to rotatory clearance between the orifice, limits the probe in the axis position of container, like this when the probe drives the paddle and rotates the stirring, can avoid the paddle to touch the inner wall of container, protection paddle and reaction vessel.

Furthermore, the upper part of the reaction hole plug head is cylindrical, the lower part of the reaction hole plug head is inverted round table-shaped, and the taper of the round table-shaped is 10 degrees. The lower end of the reaction hole plug head is provided with a taper which can be better matched and inserted into the orifice of the reaction vessel.

Further, spacing rubber buffer can dismantle with the probe and be connected, and the mounted position of spacing rubber stopper on the probe can be adjusted from top to bottom. Because in the experiment of difference, reaction vessel's length and inside solution volume are different, consequently when experimental, the paddle is inconsistent apart from the distance in container drill way, and limit rubber stopper can come to limit the length that the paddle stretched into solution through installing different positions on the probe.

Furthermore, the paddle at the lower end of the probe is oblate, the probe is connected with the edge of the oblate paddle, and the circle center of the oblate paddle is located on the axis extension line of the probe.

Furthermore, the diameter range of the oblate paddle is 3.0mm-4.0 mm.

Further, the diameter range of the probe is 0.8mm-1.2mm, and the length range of the probe is 8mm-12 mm.

Furthermore, the probe and the paddle are integrally formed, and the manufacturing material of the probe and the paddle is silicon dioxide or polystyrene.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model integrates the paddle capable of stirring the solution to the tail end of the probe, the paddle is made of silicon dioxide material, the outer surface of the paddle is coupled with the antibody or antigen required by the reaction, the surface area of the paddle is large enough because the paddle is flat, and the antibody or antigen coupled on the paddle can meet the requirement of the reaction dosage; solution can be stirred in the paddle rotation, makes solution misce bene, and behind the misce bene, spacing rubber buffer can make the paddle keep unsettled state, and the paddle is static to be placed in solution, takes place the abundant reaction with solution, and whole process need not get and puts the probe, and the paddle does not contact with outside air, consequently can avoid cross infection by the at utmost, makes the experimental data accuracy higher.

Drawings

FIG. 1 is a three-dimensional structure diagram of a stirring and detecting integrated probe of the present invention;

FIG. 2 is a front view of an integrated stirring and detecting probe according to the present invention;

labeled in the figure as: probe 1, spacing rubber buffer 2, annular spacing limit 21, place board 22, reaction hole chock plug 23, paddle 3.

Detailed Description

The invention is further described with reference to the following figures and examples.

As shown in fig. 1-2 the utility model relates to a stirring and detection integral type probe, including probe 1 and spacing rubber buffer 2, spacing rubber plug sleeve 2 is established on probe 1, and the fixed paddle 3 that is equipped with the platykurtic of probe 1's lower extreme, and 3 surfaces of paddle are decorated through 3-aminopropyl trimethylsilane, the required antibody of coupling immunodetection or antigen. The limiting rubber plug 2 is detachably connected with the probe 1, and the mounting position of the limiting rubber plug 2 on the probe 1 can be adjusted up and down; when the upper end of probe 1 was snatched the rotation, spacing rubber buffer 2 and paddle 3 were followed probe 1 simultaneously and are rotated.

The limiting rubber plug 2 is circular, the probe 1 is of a cylindrical structure, the limiting rubber plug 2 comprises a reaction hole plug head 23, a placing plate 22 and a rubber sleeve (not shown in the figure) which are concentrically arranged, the cross section of the rubber sleeve is T-shaped, the upper end of the rubber sleeve is provided with an annular limiting edge 21, the rubber sleeve is sleeved on the probe 1, the placing plate 22 and the reaction hole plug head 23 are sequentially sleeved on the outer side of the rubber sleeve from bottom to top, the diameter of the placing plate 22 is larger than the maximum diameter of the reaction hole plug head 23, and the placing plate 22 is limited between the annular limiting edge 21 and the reaction hole plug head 23; the upper part of the reaction hole plug 23 is cylindrical, the lower part is inverted round table-shaped, and the taper of the round table-shaped is 10 degrees.

The probe 1 and the paddle 3 are integrally formed, and the manufacturing material of the probe is silicon dioxide or polystyrene. In a preferred embodiment, the blade 3 at the lower end of the probe 1 is oblate, the probe 1 is connected with the edge of the oblate blade, and the center of the oblate blade 3 is positioned on the axial extension line of the probe 1. Besides, the shape of the paddle 3 can also be flat oval, flat square, flat rectangular and other axisymmetric figures, and the symmetric axis of the paddle 3 is coincided with the axis of the probe 1 during connection.

The diameter range of the oblate type blade 3 is 3.0mm-4.5mm, and the preferable range is 3.5mm-4.0 mm; the diameter range of the probe 1 is 0.2mm-2.0mm, and the preferable range is 0.8mm-1.2 mm; the length of the probe 1 is in the range of 5mm to 25mm, preferably in the range of 8mm to 12 mm.

Test procedure using this probe: during a test, a sample is added into a reaction container, a probe 1 is grabbed by a probe gripper in an instrument, an antibody or an antigen corresponding to the sample is coupled to the surface of a paddle 3 at the lower end of the probe 1, the lower end of the probe 1 is placed into the reaction container, a limiting rubber plug 2 is covered on an orifice of the reaction container, a microspur is kept between a placing plate 22 and the orifice, the gripper grabs the probe 1 to rotate, the paddle 3 rotates and is stirred in a solution, the surface of the paddle 3 reacts with the solution while stirring, the probe 1 can be put down after being uniformly stirred, the placing plate 22 is placed on the orifice of the container, and the paddle 3 continuously reacts with the solution under a static state until the reaction is finished; if necessary, the gripper can also hold the probe 1 and continue to rotate and stir until the reaction time is over. After the reaction is finished, the probe gripper takes the probe 1 out of the reaction container, the reaction container is placed into a cleaning container for cleaning, the probe 1 is driven by the gripper to rotate to stir the cleaning solution, the probe is read after the cleaning is finished, and the detection result is calculated according to the signal value.

The above description is only for the preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes and substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (8)

1. The utility model provides a stirring and detection integral type probe which characterized in that: the probe comprises a round limiting rubber plug and a cylindrical limiting rubber plug, wherein the limiting rubber plug is sleeved on the probe, a flat blade is fixedly arranged at the lower end of the probe, the surface of the blade is modified by 3-aminopropyl trimethyl silane, and an antibody or an antigen required by immunodetection is coupled; when the upper end of probe was snatched the rotation, spacing rubber buffer and paddle were rotatory along with the probe simultaneously.

2. The integrated stirring and detecting probe as claimed in claim 1, wherein: the limiting rubber plug comprises a concentric reaction hole plug head, a placing plate and a rubber sleeve, the cross section of the rubber sleeve is T-shaped, the upper end of the rubber sleeve is provided with an annular limiting edge, the rubber sleeve is sleeved on the probe, the placing plate and the reaction hole plug head are sequentially sleeved outside the rubber sleeve from bottom to top, the diameter of the placing plate is larger than the maximum diameter of the reaction hole plug head, and the placing plate is limited between the annular limiting edge and the reaction hole plug head.

3. The integrated stirring and detecting probe as claimed in claim 2, wherein: the upper part of the reaction hole plug head is cylindrical, the lower part of the reaction hole plug head is inverted round table-shaped, and the taper of the round table-shaped is 10 degrees.

4. The integrated stirring and detecting probe as claimed in claim 2, wherein: spacing rubber buffer can dismantle with the probe and be connected, and the mounted position of spacing rubber stopper on the probe can be adjusted from top to bottom.

5. The integrated stirring and detecting probe as claimed in claim 1, wherein: the paddle at the lower end of the probe is oblate, the probe is connected with the edge of the oblate paddle, and the circle center of the oblate paddle is located on the axis extension line of the probe.

6. The integrated stirring and detecting probe as claimed in claim 5, wherein: the diameter range of the oblate type blade is 3.0mm-4.0 mm.

7. The integrated stirring and detecting probe as claimed in claim 1, wherein: the diameter range of the probe is 0.8mm-1.2mm, and the length range of the probe is 8mm-12 mm.

8. The integrated stirring and detecting probe as claimed in claim 1, wherein: the probe and the paddle are integrally formed, and the manufacturing material of the probe and the paddle is silicon dioxide or polystyrene.

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