CN216411292U - A volatile organic polluted soil biological toxicity test device - Google Patents

Abstract

The utility model provides a volatile organic polluted soil biological toxicity testing device. The device is composed of a soil sample bottle, a gas washing and testing device bottle, and a peristaltic pump. The top of the gas washing and testing device bottle passes through a conduit and the soil sample. The bottom of the bottle is connected, a peristaltic pump is provided on the conduit, and the conduit connected to the top of the soil sample bottle is inserted into the bottom of the bottle through the conduit socket of the bottle body of the gas washing and test device. The device siphons the solution containing volatile pollutants into the sponge through the capillary, maintains the wetness of the sponge, guarantees the wet environmental conditions required by the white buddha, and tests the avoidance behavior of the springtail. The device can simultaneously test the influence of volatile pollutants in air and aqueous solution on white character jumping, and has high sensitivity.

Description

A kind of volatile organic polluted soil biological toxicity test device

technical field

The utility model relates to a volatile organic polluted soil biological toxicity testing device, which belongs to the field of soil biological comprehensive toxicity testing methods aiming at soil habitat function.

Background technique

Volatile organic compounds, as a special kind of soil pollutants, are listed as potentially dangerous pollutants in the environment and should be controlled first because of their complexity and harmfulness. The pollution diagnosis of site soil can provide important basis for site management decision. At present, soil pollution diagnosis in my country is mainly based on pollutant concentration analysis, which has the disadvantages of high detection cost, preset detection indicators directly affecting the detection results, and inability to reflect the comprehensive toxicity of complex multi-component pollutants.

Using sensitive organisms to directly reflect the comprehensive toxic effects of contaminated sites can characterize the comprehensive toxic effects of soil samples on individual organisms and populations. However, the existing test methods are only suitable for non-volatile organic pollution and heavy metal pollution sites, not for volatile organic pollution soils.

Bai Futiao is a representative of sensitive organisms in soil ecosystems, and is widely used in environmental hazard assessment of pesticides and contaminated soil. Because the base of Baifujing's antennae has horns, which can be used to monitor chemicals in the air, and there is a pair of abdominal tubes on the ventral surface of the first segment of the abdomen, which can communicate fluids with the external environment. Therefore, Baifujing is a good object for monitoring volatile organic pollutants. ISO has developed a number of test methods based on Collembola and other Collembola species, but these methods are suitable for non-volatile organic pollution and heavy metal pollution sites, but not for volatile organic pollution soils.

Utility model content

In order to solve the above-mentioned deficiencies in the prior art, the utility model proposes a device for testing the biological toxicity of volatile organic polluted soil.

In order to achieve the above-mentioned purpose, the device is realized through the following technical solutions:

The device is composed of three parts: a soil sample bottle, a gas washing and testing device bottle, and a peristaltic pump.

The soil sample bottle is a cylindrical glass bottle with a stopper with upper and lower openings, the stopper is provided with a conduit hole, and the bottle is provided with a sieve plate.

The gas washing and testing device bottle is composed of an upper cap with a stopper and a bottle body, the bottle stopper is provided with a conduit hole, the upper cap is provided with a glass partition, the bottle body is provided with a conduit socket, and the inside of the bottle body is provided with glass. Partitions and capillaries.

The top of the scrubbing and testing device bottle is connected to the bottom of the soil sample bottle through a conduit, the conduit is provided with a peristaltic pump, and the conduit connected above the soil sample bottle is inserted into the bottom of the bottle through the conduit socket of the scrubbing and testing device bottle body. .

Specifically, the upper opening of the soil sample bottle is larger than the lower opening, and the sieve plate is located in the soil sample bottle near the bottom.

Specifically, the glass partition inside the bottle body of the washing and testing device is located on the upper part of the bottle body and parallel to the bottom of the bottle. The airway partition of the partition, the height of the airway partition is 20mm-30mm, the small holes are evenly distributed on the glass partition, the glass partition is divided into two areas by the transverse partition, a sponge is placed on one area, and capillary tubes are inserted into the sponge. Go down perpendicular to the underside through the small hole in the glass partition, and another area is coated with volatile-free oil-based glue.

Specifically, the glass partition in the upper lid of the bottle of the test device is parallel to the bottom of the bottle, and the bottom surface of the glass partition is coated with oily glue that does not contain volatile substances.

Specifically, the sponge is black and has a thickness of 5-10 mm.

The method of using the device of the utility model is as follows: the soil to be tested is filled on the sieve plate of the soil sample bottle; the deionized water is filled in the bottle of the washing and testing device as the washing solution, and the liquid level is lower than the glass partition in the bottle body; Tighten all the stoppers, start the peristaltic pump to pump the gas above the scrubbing and test device bottle into the bottom of the soil sample bottle, and then enter the scrubbing solution in the scrubbing and test device bottle from the top, wait until the sponge on the glass partition is wet, and stop peristalsis Pump, open the top of the bottle of the scrubbing and testing device, add lepidopteran larvae on the sponge, close the cover, start the peristaltic pump, time and record the number of lepidoptery jumping out of the sponge and the remaining number of springtails on the sponge.

The volatile organic polluted soil biotoxicity test device of the utility model has the following advantages: the solution containing volatile pollutants is siphoned into the sponge through the capillary, on the one hand, the sponge is kept moist to ensure the moist environmental conditions required for the baifujing; On the other hand, it is also a test solution for testing the avoidance behavior of springtails. The holes in zone 9A allow excess solution siphoned to the sponge to leak back into the wash liquid. It can simultaneously test the influence of volatile pollutants in air and aqueous solution on the white character jump with high sensitivity. Applying oily glue without volatile substances to some areas can prevent some white characters from jumping to avoid avoidance behavior. After jumping out of the sponge, the avoidance behavior will occur again due to the influence of volatile pollutants in the air and jump back to the sponge area.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the present utility model;

Fig. 2 is a schematic diagram of the upper cover of the bottle of the scrubbing and testing device;

Figure 3 is a bottom view of the upper cover of the bottle of the scrubbing and testing device;

Figure 4 is a schematic diagram of the bottle body of the scrubbing and testing device;

Figure 5 is a top view of the bottle body of the scrubbing and testing device;

In the picture: 1- soil sample bottle, 2- soil sample containing volatile pollutants, 3- frit, 4- silica gel stopper, 5- air guide, 6- peristaltic pump, 7- cover glass partition, 8- Sponge, 9-bottle body glass partition, 10-capillary tube, 11-gas washing solution, 12-gas washing and test device bottle, 9A-bottle body glass partition sponge area, 9B-bottle body glass partition glue area, 9C-sponge area It is separated from the glue area, and the airway partition of 9D-bottle glass partition.

Detailed ways

The specific embodiments of the present invention will be described below with reference to the accompanying drawings.

A volatile organic polluted soil biotoxicity testing device, as shown in FIG. 1 , is composed of three parts: a soil sample bottle 1 , a gas scrubbing and testing device bottle 12 , and a peristaltic pump 6 .

The soil sample bottle 1 is a cylindrical glass bottle with a stopper with upper and lower openings, the upper opening is larger than the lower opening, and a sieve plate 3 is arranged in the bottle.

As shown in Figures 2-5, the bottle 12 of the gas washing and testing device is composed of an upper cap with a stopper and a bottle body. The upper cap is provided with a glass partition 7, and the lower surface is coated with oily glue that does not contain volatile substances. The bottle body is provided with a conduit socket, and the inside of the bottle body is provided with a glass partition 9 and a capillary tube 10 . The glass partition 7 is in the shape of an excellent arc, the arc edge is fitted with the bottle body, and the straight edge is provided with an airway partition 9D perpendicular to the glass partition, and the height of the airway partition is 20mm-30mm. The small holes are evenly distributed on the glass partition, which is divided into two areas by the transverse partition 9C. A black sponge with a thickness of 5-10mm is placed on one area, and a capillary tube 10 is inserted into the sponge. Underside, another area is coated with volatile-free oil-based glue.

The top of the scrubbing and testing device bottle 12 is connected to the bottom of the soil sample bottle 1 through a conduit, the conduit is provided with a peristaltic pump 6, and the conduit 5 connected above the soil sample bottle 1 passes through the gas scrubbing and testing device bottle body. Insert into the bottom of the bottle.

When using the device, first fill the soil sample bottle 1 with a quantitative amount (eg, 500 g) of soil to be tested.

In the scrubbing and testing device bottle 12 , oily glue that does not contain volatile substances is applied to the lower surface of the upper cover glass partition 7 . Place a sponge in area A on the glass partition 9 of the bottle body, apply oily glue without volatile substances in area B, place a capillary tube on the bottom of the sponge, and fill the bottom of the bottle with deionized water as a washing solution. The liquid level is lower than the bottle body. Glass partition 9.

Start the peristaltic pump 6 so that the air flow enters the bottom of the soil sample bottle 1 from the bottom, and then enters the washing and testing device bottle 12 after the soil sample bottle 1 is discharged from the top. After the bottle 12 is installed, it is returned to the soil sample bottle 1 through the peristaltic pump to enter the next cycle.

After running for 2 hours, it was observed that the sponge placed in the area A on the glass partition 9 of the bottle body had been wetted through the bottle 12 of the scrubbing and testing device. Pause the peristaltic pump 6, open the top cover of the scrubbing and testing device bottle 12, gently add N (recommended 30) 9-12-day-old larvae on the sponge, and cover the scrubbing and testing device bottle 12 Put the cover on, start the peristaltic pump, and start timing. Observe for 15 minutes, and record the number N ₁ of white characters jumping out of the sponge.

Continue to run for 48h, and record the number N ₂ of the remaining springtails on the sponge.

The calculation of the evasion rate of Zerglings is as follows:

The volatile gas circulates in the closed system, which can make the volatile pollutants gradually distribute and balance in the water, soil and air in the system. The solution containing volatile pollutants siphoned into the sponge through the capillary tube, on the one hand, maintains the moistness of the sponge and guarantees the moist environmental conditions required for the baifu jumping, and on the other hand is also a test solution for testing the avoidance behavior of springtails. When the volatile pollutants in the solution and air flow have a toxic effect on the white rune, the white rune will evade and bounce to the upper area B on the glass partition 9 of the bottle body. White Rune Jumps that jump to this area will not be able to jump again due to the oily glue applied to this area. Using this device, the effect of volatile pollutants in air and aqueous solution on white character jumps can be tested at the same time, and the sensitivity is high.

The above is only a basic implementation form of the present invention according to the technical solution, and any improvement in a similar form proposed according to the present invention should be regarded as the scope of protection of the present invention.

Claims (5)

1. The device for testing the biotoxicity of the volatile organic contaminated soil is characterized by comprising a soil sample bottle, a gas washing and testing device bottle and a peristaltic pump;

the soil sample bottle is a cylindrical glass bottle with a plug and an upper opening and a lower opening, a guide pipe hole is formed in the plug, and a sieve plate is arranged in the bottle;

the gas washing and testing device bottle consists of a bottle plug and a bottle body, wherein the bottle plug is provided with a guide pipe hole, a glass partition is arranged in the upper cover, the bottle body is provided with a guide pipe socket, and the glass partition and a capillary pipe are arranged in the bottle body;

the upper part of the gas washing and testing device bottle is connected with the bottom of the soil sample bottle through a conduit, the conduit is connected with a peristaltic pump, and the conduit connected with the upper part of the soil sample bottle penetrates through a conduit socket of the body of the gas washing and testing device bottle and is inserted into the bottom of the bottle.

2. The apparatus of claim 1, wherein the soil sample bottle has a larger upper opening than a lower opening, and wherein the screen is positioned within the soil sample bottle near the bottom.

3. The device of claim 1, wherein the glass partition inside the bottle body of the washing and testing device is positioned on the upper portion of the bottle body and parallel to the bottom of the bottle, the glass partition is in a shape of a U-arc segment, an arc edge is attached to the bottle body, an air passage partition perpendicular to the glass partition is arranged on a straight line edge, the height of the air passage partition is 20mm-30mm, small holes are uniformly distributed in the glass partition, the glass partition is divided into two areas by a transverse partition, a sponge is placed in one area, a capillary tube is inserted into the sponge, the capillary tube penetrates through the small holes in the glass partition and is perpendicular to the bottom surface downwards, and the other area is coated with oily glue without volatile substances.

4. The device according to claim 1, characterized in that the glass partition in the upper lid of the bottle of the washing and testing device is semicircular and parallel to the bottom of the bottle, and the lower side of the glass partition is coated with oily glue without volatile substances.

5. A device according to claim 3, wherein the sponge is black and 5-10mm thick.

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