CN216350316U - Micro-spectrophotometer capable of quickly measuring multiple samples - Google Patents
Micro-spectrophotometer capable of quickly measuring multiple samples Download PDFInfo
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- CN216350316U CN216350316U CN202120171884.2U CN202120171884U CN216350316U CN 216350316 U CN216350316 U CN 216350316U CN 202120171884 U CN202120171884 U CN 202120171884U CN 216350316 U CN216350316 U CN 216350316U
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- 230000007246 mechanism Effects 0.000 claims abstract description 92
- 238000001514 detection method Methods 0.000 claims abstract description 72
- 239000013307 optical fiber Substances 0.000 claims abstract description 69
- 230000003287 optical effect Effects 0.000 claims abstract description 46
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 239000010453 quartz Substances 0.000 claims description 7
- 239000000835 fiber Substances 0.000 claims description 4
- 239000000377 silicon dioxide Substances 0.000 claims 2
- 239000000523 sample Substances 0.000 abstract description 91
- 238000002835 absorbance Methods 0.000 abstract description 15
- 239000012488 sample solution Substances 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 description 10
- 238000004140 cleaning Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000011481 absorbance measurement Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model relates to a micro spectrophotometer capable of rapidly measuring a plurality of samples, which solves the problems of small number of detection samples, complex operation, long detection completion time, complex equipment and the like in the prior art, and adopts the technical scheme that: the device comprises a detection mechanism and a sample feeding mechanism, wherein the detection mechanism comprises an optical path system and an analysis module matched with the optical path system, and a sample base on the sample feeding mechanism is matched with the optical path system. The method has the following effects: the detection of a plurality of samples (4, 8, 16 and the like in different quantities) can be realized by adopting one optical path system (namely one transmitting optical fiber and one receiving optical fiber), the absorbance of a plurality of sample solutions can be measured at the same time, and the detection efficiency is improved; only one optical path system is provided, and the detection optical path is fixed, so that the detection is more stable and the detection accuracy is improved.
Description
Technical Field
The utility model relates to the field of optical measurement, in particular to a micro-spectrophotometer capable of rapidly measuring a plurality of samples.
Background
The micro-spectrophotometer measures the concentration of a sample by detecting the absorbance, and the micro-sample with the volume of 0.5-2 ul is dropped on a detection platform by utilizing the surface tension characteristic of liquid, and the detection platform can automatically measure to obtain the absorbance A:
wherein, IRLight intensity through the sample, I0The intensity of light transmitted through the blank or reference sample.
According to Beer-Lambert's law, the concentration of the solution:
wherein A is the absorbance of the sample,
epsilon is the absorbance of the sample, each sample has a fixed absorbance,
p is the optical path length, 1cm is the industry standard.
In actual measurement, the range of absorbance A obtained by the instrument is only about 0-4Abs, and the range of detectable concentration is correspondingly small. Therefore, the actual measurement often needs to be performed after the sample is diluted to a certain degree.
In order to effectively measure a small amount of sample with high concentration, the absorbance can be adjusted by adjusting the optical path P, and according to Lambert's law, the absorbance A is proportional to the optical path:
by selecting a proper optical path, the precision of the absorbance A can be effectively controlled, so that the measurement of a high-concentration sample is realized.
At present, the existing micro-spectrophotometer can accurately measure the concentration of a small amount of samples, but can only complete the detection of one sample at a time, and the existing micro-spectrophotometer consumes time when the number of samples is large. For example, one of the U.S. patents (US 2009059225a1) discloses the following technical solutions: through 8 passageways, can the single operation accomplish 8 measurements of sample, although accelerated measurement efficiency, because there is the difference in different passageways, consequently the accuracy of detection reduces to some extent.
Disclosure of Invention
The utility model aims to solve the problems in the prior art and provides a micro-spectrophotometer capable of rapidly measuring a plurality of samples, which can realize the detection of a plurality of samples (which can be 4, 8, 16 and the like in different quantities) by adopting a light path system (namely one transmitting optical fiber and one receiving optical fiber), can approach to simultaneously measure the absorbance of a plurality of sample solutions and improve the detection efficiency; only one optical path system is provided, and the detection optical path is fixed, so that the detection is more stable and the detection accuracy is improved.
The technical purpose of the utility model is mainly solved by the following technical scheme: the micro spectrophotometer capable of rapidly measuring a plurality of samples is characterized by comprising a detection mechanism and a sample feeding mechanism, wherein the detection mechanism comprises an optical path system and an analysis module matched with the optical path system, and a sample base on the sample feeding mechanism is matched with the optical path system. The detection of a plurality of samples (4, 8, 16 and the like in different quantities) can be realized by adopting one optical path system (namely one transmitting optical fiber and one receiving optical fiber), the absorbance of a plurality of sample solutions can be measured at the same time, and the detection efficiency is improved; only one optical path system is provided, and the detection optical path is fixed, so that the detection is more stable and the detection accuracy is improved.
As a further improvement and supplement to the above technical solution, the present invention adopts the following technical measures: the optical path system comprises a light source, a transmitting optical fiber with a light inlet end matched with the light source, and a receiving optical fiber with a light inlet end matched with the light outlet end of the transmitting optical fiber, wherein the sample base is matched with the light outlet end of the transmitting optical fiber and the light inlet end of the receiving optical fiber. The optical path system has only one optical path: the light input end of the transmitting optical fiber receives light emitted by the light source, the light is transmitted in the transmitting optical fiber, light rays emitted from the light output end of the transmitting optical fiber irradiate a sample, then the light rays enter the receiving optical fiber through the light input end of the receiving optical fiber, finally the light rays irradiate the analysis module from the light output end of the receiving optical fiber, the analysis module analyzes light data, and finally the detection of the sample is completed.
The detection mechanism further comprises a detection support, the transmitting optical fiber and the receiving optical fiber are fixed on the detection support, and the transmitting optical fiber and the receiving optical fiber are arranged coaxially. The transmitting optical fiber and the receiving optical fiber (detection optical path) are fixed, so that the stability of data of the detection light can be improved, and the detection accuracy is improved.
The sample feeding mechanism comprises a transverse adjusting mechanism and a longitudinal adjusting mechanism which are matched with each other, and a sample support is arranged on the transverse adjusting mechanism or the longitudinal adjusting mechanism, the transverse adjusting mechanism and the longitudinal adjusting mechanism are matched with each other to adjust the transverse position and the longitudinal position of the sample support, and the sample base is arranged on the sample support. The position of the sample is automatically adjusted through the sample feeding mechanism, and the detection efficiency is improved. And the sample feeding mechanism is separated from the optical path system, so that the operations and treatments such as sample feeding, cleaning and the like are more convenient, the optical fiber is effectively protected, and the optical path is more stable.
The horizontal adjustment mechanism sets up on the vertical adjustment mechanism, horizontal adjustment mechanism includes transverse guide and horizontal actuating mechanism, the sample support sets up on the transverse guide, horizontal actuating mechanism is used for adjusting the horizontal position of sample support, vertical adjustment mechanism includes longitudinal rail and vertical actuating mechanism, transverse guide with the cooperation of longitudinal rail, vertical actuating mechanism is used for driving transverse guide's longitudinal position.
In order to improve the accuracy of the adjusting position, a first position sensor is arranged on the transverse adjusting mechanism and used for sensing the transverse position, and a second position sensor is arranged on the longitudinal adjusting mechanism and used for sensing the longitudinal position.
The transverse driving mechanism comprises a stepping motor and a screw rod arranged on an output shaft of the stepping motor, the transverse guide rail is a miniature linear guide rail, the longitudinal driving mechanism is a miniature stepping motor, the longitudinal guide rail is a linear bearing, and the transverse guide rail and the longitudinal guide rail are arranged perpendicularly. The screw rod and the stepping motor are adopted, so that the accuracy of adjusting the position can be improved.
The sample holder is provided with a strip-shaped groove, the sample base is strip-shaped, the sample base is arranged in the strip-shaped groove, the sample base comprises a base bottom plate and a base cover plate matched with the base bottom plate, the base bottom plate and the base cover plate are both transparent quartz plates, the base bottom plate and the base cover plate are of split structures, and the base bottom plate and the base cover plate are arranged in parallel. The sample base is embedded in the strip-shaped groove, so that the assembly is convenient and the assembly reliability is good. The sample base is formed by combining a base bottom plate and a base cover plate, so that the base cover plate can be conveniently opened and closed, and the sample can be conveniently cleaned and added.
The clearance between the lower surface of base apron and the upper surface of base bottom plate is 0.2mm, 0.5mm or 1.0 mm.
The transmitting optical fiber is a 0.2 mm-specification quartz optical fiber, and the receiving optical fiber is a 0.6 mm-specification quartz optical fiber.
The utility model has the following beneficial effects:
1. the absorbance of the solution of which a plurality of samples are measured can be approached simultaneously, so that the detection efficiency can be improved;
2. only one optical path system is provided, and the detection optical path is fixed, so that the detection is more stable and the detection accuracy can be improved;
3. the sample base is driven by a precise motor to adjust the position;
4. and by matching with different sample bases, the sample solution can be more conveniently and more conveniently detected.
5. Platform that the sample was placed and detection light path separation make things convenient for the application of sample more and carry out other processings, effectively protect optic fibre, and the optical distance is more stable.
Drawings
Fig. 1 is a schematic diagram of an axial structure of the present invention.
Fig. 2 is a schematic top view of the structure of fig. 1.
Fig. 3 is a schematic cross-sectional structure of the present invention.
Fig. 4 is a partially enlarged schematic view of fig. 3.
Fig. 5 is a schematic view showing the structure of the opened state of the sample base in the present invention.
Fig. 6 is a schematic view showing a closed state of the sample base in the present invention.
Detailed Description
The technical scheme of the utility model is further specifically described by the following embodiments and the accompanying drawings.
Example (b): herein, the lateral direction refers to a direction along the length of the sample base, and the longitudinal direction refers to a direction along the width of the sample base.
As shown in fig. 1-6, the microspectrophotometer capable of rapidly measuring a plurality of samples is characterized by comprising a detection mechanism and a sample feeding mechanism, wherein the detection mechanism comprises an optical path system and an analysis module 14 matched with the optical path system, and a sample base on the sample feeding mechanism is matched with the optical path system. The detection of a plurality of samples (4, 8, 16 and the like in different quantities) can be realized by adopting one optical path system (namely one transmitting optical fiber and one receiving optical fiber), the absorbance of a plurality of sample solutions can be measured at the same time, and the detection efficiency is improved; only one optical path system is provided, and the detection optical path is fixed, so that the detection is more stable and the detection accuracy is improved.
In general, the detection mechanism, the sample feeding mechanism and the analysis module are arranged on one platform 1 for convenient operation and convenient cooperation among the mechanisms and modules.
The sample base 5 is used for carrying a sample solution, the number of the carried samples can be modified as required, and is fixed on the sample holder 6. The sample support 6 moves along with the transverse adjusting mechanism and the longitudinal adjusting mechanism, and the sample base 5 can be accurately moved to any position through the matching action of the transverse adjusting mechanism and the longitudinal adjusting mechanism.
When the micro-spectrophotometer is used, a micro-spectrophotometer capable of rapidly measuring a plurality of samples is in an initial state, and after a proper sample base 5 is selected, the micro-spectrophotometer is placed into a sample support 6 and automatically fixed. Then, a proper program is selected in the operation system (the operation system is not shown in the figure), the sample feeding mechanism can accurately move each sample solution in the sample base 5 into the detection position of the detection mechanism in sequence to complete detection, then the sample base 5 returns to the initial position, and operations such as cleaning, sample solution replacement and the like are carried out at the initial position.
As shown in fig. 3 and 4, when the sample feeding mechanism feeds the first sample on the sample base 5 to the detection position, the light source receives a signal to generate a light beam, and the light beam sequentially passes through the emission optical fiber, the sample base and the receiving optical fiber and then is transmitted to the analysis module to complete one measurement.
As a further improvement and supplement to the above technical solution, the present invention adopts the following technical measures: the optical path system comprises a light source 2, a transmitting optical fiber 3 with a light inlet end matched with the light source, and a receiving optical fiber 4 with a light inlet end matched with a light outlet end of the transmitting optical fiber, wherein the sample base 5 is matched with the light outlet end of the transmitting optical fiber 3 and the light inlet end of the receiving optical fiber 4. The optical path system has only one optical path: the light input end of the transmitting optical fiber receives light emitted by the light source, the light is transmitted in the transmitting optical fiber, light rays emitted from the light output end of the transmitting optical fiber irradiate a sample, then the light rays enter the receiving optical fiber through the light input end of the receiving optical fiber, finally the light rays irradiate the analysis module from the light output end of the receiving optical fiber, the analysis module analyzes light data, and finally the detection of the sample is completed.
In order to improve the detection effect and accuracy, the light source 2 adopts a flash xenon lamp of Japan Kongson.
The detection mechanism further comprises a detection support 15, the transmitting optical fiber and the receiving optical fiber are fixed on the detection support, and the transmitting optical fiber and the receiving optical fiber are arranged coaxially. The transmitting optical fiber and the receiving optical fiber (detection optical path) are fixed, so that the stability of data of the detection light can be improved, and the detection accuracy is improved.
The transmitting optical fiber and the receiving optical fiber are fixed on the detection bracket to keep static and stable in state, and the deviation of the space state after each closing does not exist like the rotating arm structure in the prior art. Meanwhile, the light outgoing part and the light incoming part of the two optical fibers are kept on the same axis, so that the measurement error caused by the angular deviation of the light beams is avoided.
Further preferably: the transmitting optical fiber 3 is a 0.2mm quartz optical fiber, and the receiving optical fiber 4 is a 0.6mm quartz optical fiber.
As shown in figure 1, the detection bracket is inverted L-shaped, the lower end of the detection bracket is fixed on the platform 1, the analysis module is arranged in a cavity formed by the inverted L-shaped, the upper end of the detection bracket is provided with a U-shaped transverse groove 2-1, and the height of the U-shaped transverse groove 2-1 is adapted to the smooth passing of the sample bracket 6.
The sample feeding mechanism comprises a transverse adjusting mechanism and a longitudinal adjusting mechanism which are matched with each other, and a sample support 6 arranged on the transverse adjusting mechanism or the longitudinal adjusting mechanism, the transverse adjusting mechanism and the longitudinal adjusting mechanism are matched with each other to adjust the transverse position and the longitudinal position of the sample support, and the sample base 5 is arranged on the sample support 6. The position of the sample is automatically adjusted through the sample feeding mechanism, and the detection efficiency is improved. And the sample feeding mechanism is separated from the optical path system, so that the operations and treatments such as sample feeding, cleaning and the like are more convenient, the optical fiber is effectively protected, and the optical path is more stable.
The transverse adjusting mechanism is arranged on the longitudinal adjusting mechanism, or the longitudinal adjusting mechanism is arranged on the transverse adjusting mechanism. When the transverse adjusting mechanism is arranged on the longitudinal adjusting mechanism, the specific scheme is as follows:
the transverse adjusting mechanism comprises a transverse guide rail 7 and a transverse driving mechanism 8, the sample support 6 is arranged on the transverse guide rail 7, the transverse driving mechanism 8 is used for adjusting the transverse position of the sample support, the longitudinal adjusting mechanism comprises a longitudinal guide rail 9 and a longitudinal driving mechanism 10, the transverse guide rail is matched with the longitudinal guide rail, and the longitudinal driving mechanism is used for driving the longitudinal position of the transverse guide rail.
In order to improve the accuracy of the adjustment position, a first position sensor 11 is arranged on the transverse adjusting mechanism for sensing the transverse position, and a second position sensor 12 is arranged on the longitudinal adjusting mechanism for sensing the longitudinal position. Meanwhile, the position sensors are arranged in two directions to calibrate the displacement accuracy of the sample support, so that the sample is ensured to be in the positive center of a light path during detection, and the detection error is reduced.
The transverse driving mechanism comprises a stepping motor and a screw rod arranged on an output shaft of the stepping motor, the transverse guide rail is a miniature linear guide rail, the longitudinal driving mechanism is a miniature stepping motor, the longitudinal guide rail is a linear bearing, and the transverse guide rail and the longitudinal guide rail are arranged perpendicularly. The screw rod and the stepping motor are adopted, so that the accuracy of adjusting the position can be improved.
The sample holder is provided with a strip-shaped groove 6-1, the sample base is strip-shaped, the sample base 5 is arranged in the strip-shaped groove and is relatively fixed with the sample holder, the sample base comprises a base bottom plate 5-1 and a base cover plate 5-2 matched with the base bottom plate, the base bottom plate and the base cover plate are transparent quartz plates with high light transmittance, the base bottom plate and the base cover plate are of split structures, and the base bottom plate and the base cover plate are arranged in parallel. The sample base is embedded in the strip-shaped groove, so that the assembly is convenient and the assembly reliability is good. The sample base is formed by combining a base bottom plate and a base cover plate, so that the base cover plate can be freely opened and closed, and the sample base can be cleaned and added in an open state. The opening means includes rotating the base cover, etc., and the means for vertically moving the base cover is employed in this example.
As shown in fig. 6, a gap a between the lower surface of the base cover and the upper surface of the base bottom plate is 0.2mm, 0.5mm, or 1.0 mm. That is, the gap a between the base bottom plate and the base cover in the closed state is 0.2mm, 0.5mm, 1.0mm, or the like, and is not limited to the three dimensions illustrated. When the sample base is in a closed state, the sample solution dripped on the upper surface of the base bottom plate can be automatically adsorbed on the lower surface of the base cover plate, and the sample solution forms a cylinder-like liquid column 13 due to surface tension. At this time, the measurement optical path of the sample solution becomes the distance between the susceptor cover plate (8-1) and the susceptor base plate (8-2). The fixation of the optical path, although the measurement range of a single detection is smaller, the stability of the detection is relatively improved.
When the beam formed is much smaller than the sample liquid column 13, an accurate absorbance measurement can be performed. When the light beam I0After passing through the emitting optical fiber 3, the light enters the base cover plate 5-1 → the sample solution 13 → the base bottom plate 5-2, and is emitted to obtain a light beam IRAnd then transmitted by the receiving fiber 4 to the analysis module 14. Through a light beam I0And IRCalculating the absorbance of the obtained sample
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention. In the above embodiments, the present invention may be variously modified and changed. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The micro-spectrophotometer capable of rapidly measuring a plurality of samples is characterized by comprising a detection mechanism and a sample feeding mechanism, wherein the detection mechanism comprises an optical path system and an analysis module matched with the optical path system, a sample base on the sample feeding mechanism is matched with the optical path system, the optical path system comprises a light source, an emission optical fiber with a light inlet end matched with the light source, and a receiving optical fiber with a light inlet end matched with a light outlet end of the emission optical fiber, and the sample base is matched with the light outlet end of the emission optical fiber and the light inlet end of the receiving optical fiber; the sample feeding mechanism comprises a transverse adjusting mechanism and a longitudinal adjusting mechanism which are matched with each other, and a sample support is arranged on the transverse adjusting mechanism or the longitudinal adjusting mechanism, the transverse adjusting mechanism and the longitudinal adjusting mechanism are matched with each other to adjust the transverse position and the longitudinal position of the sample support, and the sample base is arranged on the sample support.
2. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 1, wherein the detection mechanism further comprises a detection support, the transmitting optical fiber and the receiving optical fiber are fixed on the detection support, and the transmitting optical fiber and the receiving optical fiber are coaxially arranged.
3. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 2, wherein the lateral adjustment mechanism is disposed on the longitudinal adjustment mechanism, the lateral adjustment mechanism comprises a lateral guide rail on which the sample holder is disposed and a lateral drive mechanism for adjusting the lateral position of the sample holder, the longitudinal adjustment mechanism comprises a longitudinal guide rail cooperating with the longitudinal guide rail and a longitudinal drive mechanism for driving the longitudinal position of the lateral guide rail.
4. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 3, wherein the lateral adjustment mechanism is provided with a first position sensor for sensing lateral position and the longitudinal adjustment mechanism is provided with a second position sensor for sensing longitudinal position.
5. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 4, wherein the lateral driving mechanism comprises a stepping motor and a lead screw arranged on the output shaft of the stepping motor, the lateral guide is a micro linear guide, the longitudinal driving mechanism is a micro stepping motor, the longitudinal guide is a linear bearing, and the lateral guide is arranged perpendicular to the longitudinal guide.
6. A micro-spectrophotometer according to any one of claims 2 to 5 and capable of rapidly measuring a plurality of samples, wherein the sample holder is provided with an elongated groove, the sample base is elongated, the sample base is provided in the elongated groove, the sample base comprises a base bottom plate and a base cover plate matched with the base bottom plate, the base bottom plate and the base cover plate are both transparent quartz plates, the base bottom plate and the base cover plate are of split structure, and the base bottom plate and the base cover plate are arranged in parallel.
7. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 6, wherein the gap between the lower surface of the base cover plate and the upper surface of the base bottom plate is 0.2mm, 0.5mm, or 1.0 mm.
8. A microspectrophotometer capable of rapidly measuring multiple samples according to claim 7, wherein the transmitting optical fiber is 0.2mm silica fiber and the receiving optical fiber is 0.6mm silica fiber.
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