CN216284937U - A high-efficiency dual-channel circularly polarized fluorescence spectroscopy measurement system - Google Patents

Abstract

The utility model relates to the field of sample optical detection, in particular to a high-efficiency dual-channel circularly polarized fluorescence spectrum measurement system, wherein a white light source, a sample to be measured, an objective lens, a dichroic mirror, a movable mirror, a quarter wave plate, a 50 : 50 beam splitter, Glan prism, high-pass filter, focusing mirror and Y-type fiber input end are arranged in a line in turn. When the microscopic phase is formed, the movable mirror two is moved into the system. When the circularly polarized fluorescence spectrum is measured, it can be The second moving mirror is moved out of the system, the continuous laser emitted by the excitation light source is depolarized into natural light by the depolarizer, the natural light is reflected by the dichroic mirror and focused by the objective lens to the tested sample, and the tested sample is excited to emit circularly polarized fluorescence, circularly polarized fluorescence The 1/4 wave plate is converted into linearly polarized fluorescence, and the linearly polarized fluorescence is incident to the fluorescence spectrum acquisition system through the circularly polarized fluorescence acquisition module. The utility model can collect the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence of the tested sample at the same time, which greatly improves the system efficiency.

Description

A high-efficiency dual-channel circularly polarized fluorescence spectroscopy measurement system

technical field

The utility model relates to the field of sample optical detection, in particular to a high-efficiency dual-channel circularly polarized fluorescence spectrum measurement system.

Background technique

The circularly polarized fluorescence spectrum measurement system is a process of collecting the circularly polarized fluorescent light intensity of the homogeneous radiation after exciting the characteristic spectrum of the sample molecule with the depolarized excitation light. Detection of circularly polarized fluorescence spectra can assist in analyzing the luminescent properties of materials.

In the reported circularly polarized fluorescence spectrum measurement system, the excitation light source can be selected from xenon lamp or LED light source. Excitation, it is impossible to focus the excitation light small enough to meet the measurement of circularly polarized fluorescence spectroscopy at a specific position of the unevenly distributed sample, and it cannot achieve the requirements of in-situ in-situ in multiple measurements. In order to solve the above problems, consider The laser light source is used as the excitation light source. Due to the good monochromaticity of the laser, a smaller focal diameter can be obtained during focusing, so that when the focal point is located at a specific position of the sample to be measured, the circularly polarized fluorescence spectrum measurement can be carried out on the area selectively. In conjunction with microscopic imaging technology, in-situ circularly polarized fluorescence spectroscopy measurement in multiple measurements can also be achieved.

In the polarization characteristic analysis of the circularly polarized fluorescence spectrum, the method of collecting the left-handed fluorescence spectrum and the right-handed fluorescence spectrum in the sample fluorescence is usually used successively, and the asymmetric coefficient of the spectrum is obtained by mathematical analysis. Right-handed fluorescence spectrum, the acquisition speed is slow.

Through the above analysis, it can be found that among the existing achievements, the measurement of high-efficiency circularly polarized fluorescence spectroscopy still needs to be solved.

Utility model content

The purpose of the utility model is to provide a high-efficiency circularly polarized fluorescence spectrum measurement system, which uses microscopic imaging to realize the microscopic imaging and focusing of the sample area before measurement, and realizes the circular polarization of the specific position of the unevenly distributed sample by this method. Fluorescence measurement, and provides a method that can simultaneously collect the left-handed fluorescence spectrum and the right-handed fluorescence spectrum of the sample to be tested, so as to improve the collection speed.

The technical scheme that the utility model adopts for realizing the above-mentioned purpose is:

A high-efficiency dual-channel circularly polarized fluorescence spectroscopy measurement system, comprising:

Laser light source generation system, dichroic mirror, objective lens, white light source, movable mirror 2, 1/4 wave plate, 50:50 beam splitter, circularly polarized fluorescence acquisition module 1, circularly polarized fluorescence acquisition module 2, Y type Optical fiber, spectrum acquisition system and microscope imaging system;

Among them: white light source, objective lens, dichroic mirror, movable mirror 2, 1/4 wave plate, 50:50 beam splitter and circularly polarized fluorescence acquisition module 1 are arranged in a line in turn;

The light source generating system is arranged on the incident light path of the dichroic mirror, and a space for placing the tested sample is arranged between the objective lens and the white light source;

A microscopic imaging system is provided on the reflected light path of the movable mirror 2;

The 50:50 beam splitter is provided with a first circularly polarized fluorescence collection module on the transmitted light path, and a second circularly polarized fluorescence collection module on the reflected light path. The transmitted light path and the reflected light path of the 50:50 beam splitter are perpendicular to each other. and the circularly polarized fluorescence acquisition module 1 and the circularly polarized fluorescence acquisition module 2 are connected to the spectrum acquisition system through a Y-type optical fiber.

When performing microscopic imaging on the sample to be tested, the movable mirror two is located between the dichroic mirror and the 1/4 wave plate, and the white light emitted by the white light source illuminates the sample to be tested, so that the sample to be tested passes through the objective lens, The dichroic mirror and the movable mirror are then imaged in a microscope imaging system.

When the circularly polarized fluorescence spectrum is measured on the sample to be tested, the movable mirror two is moved out of a line arrangement, and the natural excitation beam emitted by the laser light source generation system passes through the dichroic mirror and the objective lens in sequence on the sample to be measured, so that the measured sample is irradiated. After the sample is excited, a circularly polarized fluorescence beam is emitted, which passes through the objective lens, the dichroic mirror, and the 1/4 wave plate in turn, and is split into the circularly polarized fluorescence acquisition module 1 and the circularly polarized fluorescence acquisition module 2 by the 50:50 beam splitter. It is sent to the spectrum acquisition system through the Y-type fiber.

The laser light source generation system includes a continuous laser light source 1 and a reflector 1, a reflector 2, a reflector 3, and a depolarizer sequentially arranged on the optical path of the continuous laser beam emitted by the continuous laser light source 1, so that the continuous laser light source 1 emits The continuous laser beam is depolarized by the depolarizer to become a natural excitation beam and is injected into the dichroic mirror.

It also includes the second continuous laser light source and the first movable reflector. When the movable reflector one is set between the first reflector and the second reflector, the continuous laser beam emitted by the second continuous laser light source will pass through the movable reflector one and the second one in turn. The second mirror, the third mirror, and the depolarizer depolarize the natural excitation beam into the dichroic mirror.

The first circularly polarized fluorescence acquisition module and the second circularly polarized fluorescence acquisition module include a Glan prism, a high-pass filter and a focusing mirror, and the circularly polarized fluorescent light beams split by the 50:50 beam splitter sequentially pass through the Glan prism. , high-pass filter and focusing mirror into the Y-fiber.

The microscopic imaging system includes four reflecting mirrors, three focusing mirrors, a CCD camera, a second data transmission line, and a second computer, wherein the CCD camera is connected to the second computer through the second data transmission line, and the fourth reflecting mirror, focusing The mirror 3 and the CCD camera are sequentially arranged on the reflected light path of the movable mirror 2.

The spectrum acquisition system includes a CCD spectrometer, a first data transmission line, and a first computer that are connected in sequence, and the CCD spectrometer is respectively connected with a circularly polarized fluorescence acquisition module 1 and a circularly polarized fluorescence acquisition module 2 through a Y-type optical fiber.

The Y-type optical fiber includes a Y-type fiber input end 1, a Y-type fiber input fiber 1, a Y-type fiber input end 2, a Y-type fiber input fiber 2, a Y-type fiber input end fusion splicing point, a Y-type fiber output fiber, Y-type optical fiber output end, wherein, the circularly polarized fluorescence acquisition module one is connected to the Y-type optical fiber input end fusion point through the Y-type optical fiber input end one and the Y-type optical fiber input fiber one in turn; The second fiber input end and the second Y-type fiber input fiber are connected to the fusion point of the Y-type fiber input end, and the Y-type fiber input end fusion point is connected to the spectrum acquisition system through the Y-type fiber output fiber and the Y-type fiber output end in turn.

The sample to be tested is one of a spin-coated solid sample, a high-pressure diamond anvil module, and a sample with an ambient temperature lower than the set room temperature.

The utility model has the following beneficial effects and advantages:

1. The present utility model is provided with a movable mirror 1 and a movable mirror 2, and the movable mirror 1 can realize the function of switching the excitation wavelength. When the movable mirror 1 is moved into the system, the continuous laser light source 2 serves as the excitation source. The light source, when the movable mirror 2 is moved out of the system, the continuous laser light source 1 is used as the excitation light source, and the movable mirror 2 can realize the switching function between fluorescence spectrum acquisition and microscopic imaging. When the movable mirror 2 is moved into the system, The sample to be tested is imaged in the microscopic imaging system, which can perform microscopic imaging and micro-focusing of the sample. When the movable mirror 2 is moved out of the system, the sample to be tested is excited by the excitation light source, and the spectrum acquisition system detects the left-handed fluorescence of the sample. Spectra were collected with dextrorotatory fluorescence spectra.

2. The utility model is provided with Glan prism one and Glan prism two whose optical axes are perpendicular to each other, and the angle between the 1/4 wave plate optical axis and the Glan prism one and Glan prism two in the system is 45°. Therefore, the system can collect the left-handed fluorescence spectrum and the right-handed fluorescence spectrum of the tested sample at the same time, which improves the collection efficiency of the system and makes up for the inefficiency of the prior art that the left-handed fluorescence light level and the right-handed fluorescence spectrum must be collected successively.

Description of drawings

Fig. 1 is the structural representation of the present utility model;

Fig. 2a is the positional relationship between the optical axis of a Glan prism and the optical axis of a quarter wave plate of a circularly polarized fluorescence collection module of the present utility model;

Fig. 2b is the positional relationship between the optical axis of the second Glan prism and the optical axis of the 1/4 wave plate of the circularly polarized fluorescence collection module of the present utility model;

Figure 3a is a schematic diagram of the fiber arrangement of the Y-type fiber input end and the output end of the present utility model;

Figure 3b is a schematic diagram of the optical fiber arrangement of the Y-type optical fiber input end and the output end of the present utility model;

Figure 3c is a schematic diagram of the optical fiber arrangement of the Y-type optical fiber input end and the output end of the present utility model;

4 is a schematic diagram of spectrum collection of the CCD spectrometer of the present utility model;

5 is a schematic diagram of the circularly polarized fluorescence spectrum of the tested sample obtained by the utility model;

Among them, 1 is continuous laser light source one, 2 is continuous laser light source two, 3 is mirror one, 4 is movable mirror one, 5 is mirror two, 6 is mirror three, 7 is depolarizer, and 8 is Dichroic mirror, 9 is the objective lens, 10 is the sample to be tested, 11 is the white light source, 12 is the movable mirror 2, 13 is the 1/4 wave plate, 14 is the 50:50 beam splitter, 15 is the Glan prism One, 16 is high-pass filter one, 17 is focusing mirror one, 18 is Y-type fiber input end one, 19 is Y-type fiber input fiber one, 20 is Glan prism two, 21 is high-pass filter two, 22 is Focusing mirror two, 23 is the Y-type fiber input end two, 24 is the Y-type fiber input fiber two, 25 is the Y-type fiber input end fusion splicing point, 26 is the Y-type fiber output fiber, 27 is the Y-type fiber output end, 28 is the CCD spectrometer, 29 is the first data transmission line, 30 is the first computer, 31 is the reflecting mirror four, 32 is the focusing mirror three, 33 is the CCD camera, 34 is the second data transmission line, 35 is the second computer, 181 is the 221 is the enlarged schematic cross-section of the Y-type optical fiber input end 18, 221 is the enlarged schematic cross-sectional view of the Y-type optical fiber input end 22, 271 is the cross-sectional enlarged schematic diagram of the Y-type optical fiber output end, 281 is a concave mirror, and 282 is a beam splitter grating , 283 is a double-row CCD array, and 2831 is an enlarged schematic diagram of the CCD array.

Detailed ways

The present utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments.

As shown in Figure 1, the utility model includes 1, a continuous laser light source, 1, a continuous laser light source two, 3, a reflector, 4, a movable reflector, 5, a reflector two, 6, a reflector three, 7. Depolarizer, 8. Dichroic mirror, 9. Objective lens, 10. Test sample, 11. White light source, 12. Movable mirror 2, 13. 1/4 wave plate, 14, 50:50 minutes Beam mirror, circularly polarized fluorescence acquisition module 1, circularly polarized fluorescence acquisition module 2, Y-type optical fiber, spectrum acquisition system and microscopic imaging system, wherein white light source 11, sample to be tested 10, objective lens 9, dichroic mirror 8, can be The second movable mirror 12, the 1/4 wave plate 13, the 50:50 beam splitter 14 and the circularly polarized fluorescence acquisition module are arranged in a line, that is, they are arranged in sequence along the optical path. The second mirror 12 is moved into the system, and the white light emitted by the white light source 11 illuminates the tested sample 10. The tested sample 10 is imaged in the microscope imaging system after passing through the objective lens 9, the dichroic mirror 8 and the movable mirror 2 12. When the sample to be tested is measured by circularly polarized fluorescence spectrum, the movable mirror 12 is moved out of the system, and the movable mirror 1 is switched between the continuous laser light source 1 and the continuous laser light source 2 2 as required. When the movable mirror 1 is moved into the system, The continuous laser light source 22 is used as the excitation light source. When the movable mirror 1 is moved out of the system, the continuous laser light source 1 is used as the excitation light source. After the excitation light source is selected, the continuous laser beam emitted by the excitation light source passes through the mirror 2. 5 and reflecting mirror 3 6 are reflected into the depolarizer 7, and depolarized by the depolarizer 7 into a natural excitation beam with no polarization characteristics. The wavelength of the natural excitation beam is lower than the threshold wavelength of the dichroic mirror 8. The natural excitation beam is incident on the dichroic mirror 8. The dichroic mirror 8 reflects the natural excitation beam to the tested sample 10, and the tested sample 10 emits a circularly polarized fluorescent light beam after being excited by the natural excitation beam. The wavelength of the polarized fluorescent light beam is higher than the threshold wavelength of the dichroic mirror 8 and is transmitted through the dichroic mirror 8, and the circularly polarized fluorescent light beam transmitted through the dichroic mirror 8 is transmitted through the 1/4 wave plate 13 and converted into linearly polarized fluorescent light The linearly polarized fluorescent beam is incident on a 50:50 beam splitter 14, and the 50:50 beam splitter splits the linearly polarized fluorescent beam into a transmitted linearly polarized fluorescent beam and a reflected linearly polarized fluorescent beam with equal energy. The transmitted linearly polarized fluorescent light beam and the reflected linearly polarized fluorescent light beam are respectively incident on the circularly polarized fluorescence collection module 1 and the circularly polarized fluorescence collection module 2, and the circularly polarized fluorescence collection module 1 and the circularly polarized fluorescence collection module Module 2 separately collects the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence in the fluorescence beam of the sample 10 excited and radiated, and inputs the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence to the input end of the Y-type fiber The first 18 and the Y-type optical fiber input terminal 2 23, the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence are transmitted through the Y-type optical fiber, and then input to the spectrum acquisition system. Left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence The light spectrum is collected and analyzed, and finally the circularly polarized fluorescence spectrum information of the tested sample 10 is obtained. The sample to be tested (10) can be a spin-coated solid sample, a high-pressure module for a diamond anvil, or a vacuum low-temperature sample. Among them, the high pressure refers to the pressure of the environment where the sample to be tested is higher than the standard atmospheric pressure, and the environment is provided by the diamond anvil. Low temperature refers to an ambient temperature with a temperature lower than room temperature, which is provided by a vacuum chamber with a cooling rod.

As shown in Figures 2a-2b, when the sample under test is excited by 10 times the excitation light to radiate a circularly polarized fluorescent beam, the circularly polarized fluorescent beam consists of a left-handed circularly polarized beam and a right-handed circularly polarized beam. The light beam and the right-handed circularly polarized light beam have different optical loss rotation directions, so when the left-handed circularly polarized light beam and the right-handed circularly polarized light beam pass through the 1/4 wave plate 13, they are converted into two linearly polarized light beams whose polarization directions are perpendicular to each other , by adjusting the optical axis directions of Glan prism one 15 and Glan prism two 20, so that the optical axis directions of Glan prism one 15 and Glan prism two 20 are perpendicular to each other and the transmittance is the largest, at this time transmitted through Glan prism The linearly polarized light beams of the first 15 and the second Glan prism 20 correspond to the left-handed circularly polarized light speed and the right-handed circularly polarized light beam of the circularly polarized fluorescent light beam, respectively.

As shown in Figures 3a to 3c, the Y-shaped optical fiber input end 18 and the Y-shaped optical fiber input end 2 23 are both formed by splicing 7 optical fibers, the 7 optical fibers are arranged in a circle, and the Y-shaped optical fiber output end The head 27 is formed by splicing 14 optical fibers, the 14 optical fibers correspond to the 7 optical fibers of the Y-type optical fiber input end one 18 and the 7 optical fibers of the Y-type optical fiber input end two 23, and the 14 optical fibers are linear. Vertical arrangement, wherein the 7 optical fibers corresponding to the Y-type optical fiber input end one 18 are arranged on the upper part of the 14 optical fibers in the linear arrangement, and the 7 optical fibers corresponding to the Y-type optical fiber input end 2 23 are arranged in the above. The lower part of the 14 fibers arranged linearly.

As shown in FIG. 1 , after the linearly polarized fluorescent beam transmitted through the Glan prism-15 is transmitted through the high-pass filter 18, the excitation beam doped in the linearly polarized fluorescent beam is filtered out, and the gold Y-type fiber is focused by the focusing mirror 17 Input end one 18, the fiber arrangement in the Y-type fiber input end 18 is shown in Figure 3, the focused linearly polarized fluorescent beam can be coupled into the Y-type fiber input end 18 with high efficiency, and transmitted through the Glan prism The linearly polarized fluorescent light beams of the two 20 are incident into the Y-type fiber input end 23 in the same principle.

As shown in Figure 4, the Y-type optical fiber output end 27 is formed by splicing 14 optical fibers in a linear arrangement. The Y-type fiber is transmitted to the Y-type fiber output end 27, and the Y-type fiber output end 27 is coupled into the CCD spectrometer 28. The CCD spectrometer 28 is composed of a concave mirror 281, a beam splitter 282 and a double-row CCD array 283. The Y-type fiber output end 27 The divergent fluorescent light beam emitted by the concave mirror 281 is collimated and transmitted to the beam splitting grating 282. The beam splitting grating 282 splits the collimated fluorescent beam in the wavelength direction, and the split fluorescent beam is incident on the double-row CCD. Array 283, the double-row CCD array 283 detects the intensity of the fluorescent beam after the splitting, and converts the light intensity into an electrical signal through photoelectric conversion. The upper row array in the double row CCD array 283 is detected, and the fluorescent light beams output by the 7 fibers in the lower part of the Y-type fiber output end 27 are detected by the lower row array in the double row CCD array 283, so the double row CCD array 283 can At the same time, the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence in the circularly polarized fluorescence beam of the excitation radiation of the sample 10 to be tested are detected.

As shown in FIG. 1 , the CCD spectrometer 28 simultaneously collects the spectrum of the left-handed circularly polarized fluorescence and the right-handed circularly polarized fluorescence of the tested sample 10 . The optical signal and the right-handed circularly polarized fluorescence are converted into electrical signals, and the electrical signals are processed by the first computer 30 to obtain the left-handed circularly polarized fluorescence spectrum and the right-handed circularly polarized fluorescence spectrum of the tested sample 10 .

The working principle of the utility model is:

As shown in FIG. 1, during the microscopic imaging process, the movable mirror 2 12 is moved into the system, the white light source 11 illuminates the sample 10 to be tested, and the sample 10 to be tested passes through the objective lens 9, the dichroic mirror 8 and the movable reflector After the mirror 12 is imaged in a microscopic imaging system, the microscopic imaging system is composed of a mirror four 31, a focusing mirror three 32, a CCD camera 33, a second data transmission line 34 and a second computer. On the translation stage with three moving degrees of freedom, , Y and Z, during the microscopic imaging process, the degrees of freedom of the XYZ are adjusted by adjusting the adjustment knob of the translation stage, and the position of the sample 10 to be tested is adjusted accordingly. The sample 10 to be tested can form a clear and complete image on the CCD camera 33, and the second computer 35 displays the clear and complete image.

As shown in Figure 1, during the acquisition of the circularly polarized fluorescence spectrum, the movable mirror 2 and 12 times are moved out of the system, and the movable mirror 1 is moved into or out of the system to switch the excitation light source. In the system, the continuous laser light source 2 2 is used as the excitation light source of the system. When the movable mirror 1 is moved out of the system, the continuous laser light source 1 is used as the excitation light source of the system. The continuous laser light emitted by the excitation light source passes through the mirror 1 5 and After the reflection of the mirror 2 6, it is transmitted through the depolarizer 7, and the depolarizer depolarizes the continuous laser light into a natural light beam without polarization characteristics. Focusing on the sample to be tested 10, the sample 10 to be tested radiates circularly polarized fluorescence after being excited by the natural light beam, and the circularly polarized fluorescence is composed of left-handed circularly polarized fluorescence and right-handed circularly polarized fluorescence.

As shown in Fig. 2a to Fig. 2b, during the polarization conversion process of the circularly polarized fluorescence, the circularly polarized fluorescence is collimated by the objective lens 9 and then transmitted through the dichroic mirror 8, and then incident on the 1/4 wave plate 13 and the 1/4 wave The sheet 13 converts the circularly polarized fluorescence into linearly polarized fluorescence whose polarization directions are perpendicular to each other, and the linearly polarized fluorescence whose polarization directions are perpendicular to each other is split into a transmitted beam and a reflected beam with equal energy by a 50:50 beam splitter 14. The transmitted light beam and the reflected light beam are respectively incident on the left-hand circularly polarized fluorescence collection module and the right-handed circularly polarized fluorescence collection module, and the Glan prism 15 and the Glan prism 20 in the left-hand circularly polarized fluorescence collection module and the right-hand circularly polarized fluorescence collection module are respectively Transmission of the linearly polarized fluorescence whose polarization directions are perpendicular to each other, the linearly polarized fluorescence transmitted through the Glan prism 15 and the Glan prism 20 is filtered by the high-pass filter 16 and the high-pass filter 21, and the wavelength is greater than the high-pass filter 16 and the high-pass filter 21. The linearly polarized fluorescence of the threshold wavelength can be transmitted through the high-pass filter 16 and the high-pass filter 21, and the linearly polarized fluorescence transmitted through the high-pass filter 16 and the high-pass filter 21 is focused into the Y-type by focusing mirror one 17 and focusing mirror two 22. Fiber input end 18 and Y-shaped fiber input end 23 .

As shown in Figures 3a-3c, in the process of fluorescence collection and propagation, the Y-type optical fiber consists of two input optical fibers and one output optical fiber. The input optical fiber is formed by splicing seven optical fibers, and the seven optical fibers show The arrangement in which 6 optical fibers are arranged around the center 1 optical fiber, the output optical fiber is formed by splicing 14 optical fibers, the 14 optical fibers are respectively from the 2 input optical fibers, and the 14 optical fibers are perpendicular to the line During the fluorescence collection process, the linearly polarized fluorescence is focused into the Y-type fiber input end 18 and the Y-type fiber input end 23 through the focusing mirror 17 and the focusing mirror 2 22, and is focused into the Y-type fiber input end. The linearly polarized fluorescence of the head 18 and the Y-type fiber input end 23 is transmitted through the Y-type fiber and then output through the Y-type fiber output end 27, and the linearly polarized fluorescence output through the Y-type fiber output end 27 is coupled into the CCD spectrometer. 28.

As shown in FIG. 4 , in the process of spectrum collection and analysis, after the fluorescence output from the Y-shaped fiber output end 27 is coupled into the CCD spectrometer 28, the fluorescence is an angular divergent beam with the Y-shaped fiber output end 27 as the vertex, so The angularly divergent beam is collimated by the concave mirror 281 into a parallel beam and then transmitted to the beam splitting grating 282. The beam splitter 282 performs horizontal diffraction and splitting of the parallel beam in the wavelength direction, and the horizontal diffraction beam is transmitted to the double-row beam. The CCD array 283 and the double-row CCD array 283 convert the light intensity information of the diffracted beam into electrical signals. Since the 14 fibers in the Y-type fiber output end 27 are linearly and vertically arranged, after a series of After collimation and diffraction, the light beams incident on the double-row CCD array 283 are wavelength-split in the horizontal direction, and remain consistent with the vertical arrangement of the 14 fibers of the Y-type fiber output end 27 in the vertical direction.

As shown in FIG. 1, in the process of signal collection and processing, after the CCD spectrometer 28 converts the input fluorescent light into electrical signals, the electrical signals are transmitted to the first computer 30 through the first data transmission line 29, and the first computer 30 processes the The electrical signal is generated and visualized.

通过对所述非对称系数的分析，即可获得被测样品10的圆偏振荧光光谱特性。As shown in FIG. 5 , the bottom curve shows the linearly polarized fluorescence spectrum corresponding to the left-handed circularly polarized fluorescent beam of the tested sample 10 displayed by the software, wherein the abscissa is the wavelength and the ordinate is the linearly polarized fluorescence spectrum of the corresponding wavelength Relative light intensity, i.e. _IL , the middle curve is the difference between the relative light intensity of the left-handed circularly polarized fluorescent beam spectrum and the right-handed circularly polarized fluorescent beam spectral relative light intensity of the sample 10 to be tested, that is, Δ= _IL - _IR , the top curve is the asymmetry coefficient of the fluorescence spectrum of the tested sample, namely

By analyzing the asymmetric coefficient, the circularly polarized fluorescence spectral characteristics of the sample 10 to be tested can be obtained.

Before using the utility model to measure the circularly polarized fluorescence spectrum of the sample to be tested, it is necessary to use the standard sample to perform system calibration. When the utility model is used to measure the sample to be tested, after the microscopic imaging of the sample to be tested and the focusing of the selected area are completed, Then carry out the circularly polarized fluorescence spectrum measurement of the tested sample.

In this embodiment, the continuous laser light source 1 is preferably a continuous laser with a wavelength of 355 nm, the continuous laser light source 2 is preferably a continuous laser with a wavelength of 405 nm, the dichroic mirror 8 is preferably a critical wavelength of 410 nm, and the objective lens 9 is preferably a focal length of 200 nm. It is preferably a diamond anvil high-voltage module, the white light source 11 is preferably an LED white light source, and the high-pass filter 16 and the high-pass filter 21 are preferably a critical wavelength of 430 nm.

Claims (10)

1. a high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system, is characterized in that, comprising: Laser light source generation system, dichroic mirror (8), objective lens (9), white light source (11), movable mirror two (12), 1/4 wave plate (13), 50:50 beam splitter (14) ), circularly polarized fluorescence acquisition module 1, circularly polarized fluorescence acquisition module 2, Y-type optical fiber, spectrum acquisition system and microscopic imaging system; Among them: white light source (11), objective lens (9), dichroic mirror (8), movable mirror two (12), 1/4 wave plate (13), 50:50 beam splitter (14) and circular The polarized fluorescence acquisition modules are arranged one by one in a line; The light source generating system is arranged on the incident light path of the dichroic mirror (8), and a space for placing the sample to be tested (10) is arranged between the objective lens (9) and the white light source (11); A microscopic imaging system is provided on the reflected light path of the movable mirror two (12); The 50:50 beam splitter (14) is provided with a first circularly polarized fluorescence collection module on the transmitted light path, and a second circularly polarized fluorescence collection module on the reflected light path. The 50:50 beam splitter (14) has a transmitted light path and the reflected light path are perpendicular to each other; the first circularly polarized fluorescence collection module and the second circularly polarized fluorescence collection module are connected to the spectrum collection system through a Y-type optical fiber. 2. A kind of high-efficiency dual-pass circularly polarized fluorescence spectroscopy measurement system according to claim 1, characterized in that, when the sample to be tested is microscopically imaged, the movable mirror two (12) are located at the dichroic mirror Between (8) and the 1/4 wave plate (13), the white light emitted by the white light source (11) illuminates the sample to be tested (10), so that the sample to be tested passes through the objective lens (9) and the dichroic mirror (8) in sequence. , and the movable mirror two (12) are then imaged in the microscope imaging system. 3. A kind of high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system according to claim 1, is characterized in that, when the measured sample is measured by circularly polarized fluorescence spectrum, the movable mirror two (12) is moved out of one line to arrange , the natural excitation beam emitted by the laser light source generating system passes through the dichroic mirror (8) and the objective lens (9) in turn and irradiates the sample (10) under test, so that the sample (10) is excited and emits a circularly polarized fluorescent beam, Passing through the objective lens (9), the dichroic mirror (8), the 1/4 wave plate (13) in turn, and the beam splitting through the 50:50 beam splitter (14) to the circularly polarized fluorescence acquisition module 1 and the circularly polarized fluorescence acquisition module 2 , and then sent to the spectrum acquisition system through the Y-type fiber. 4. A high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system according to claim 1, wherein the laser light source generating system comprises a continuous laser light source one (1) and a continuous laser light source one (1) in turn. Mirror one (3), mirror two (5), mirror three (6), and depolarizer (7) on the optical path of the continuous laser beam emitted by the continuous laser light source make the continuous laser beam emitted by the continuous laser light source one (1) pass through. The depolarizer (7) depolarizes the natural excitation beam into the dichroic mirror (8). 5. A kind of high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system according to claim 4, is characterized in that, also comprises continuous laser light source two (2) and movable mirror one (4), when movable mirror one (4) (4) When set between the first reflector (3) and the second reflector (5), the continuous laser beam emitted by the continuous laser light source two (2) passes through the movable reflector one (4) and the second reflector (5) in turn. 5) The third mirror (6) and the depolarizer (7) depolarize the natural excitation light beam into the dichroic mirror (8). 6. a kind of high-efficiency dual-channel circularly polarized fluorescence spectrum measurement system according to claim 1, is characterized in that, described circularly polarized fluorescence acquisition module 1 and circularly polarized fluorescence acquisition module 2 both comprise Glan prism, high-pass filter and Focusing mirror, the circularly polarized fluorescent light beam split by the 50:50 beam splitter (14) sequentially passes through the Glan prism, the high-pass filter and the focusing mirror and then enters the Y-type optical fiber. 7. A high-efficiency dual-pass circularly polarized fluorescence spectroscopy measurement system according to claim 1, wherein the microscopic imaging system comprises four reflecting mirrors (31), three focusing mirrors (32), and a CCD camera (33). ), a second data transmission line (34), and a second computer (35), wherein the CCD camera (33) is connected with the second computer (35) through the second data transmission line (34), and the reflecting mirrors (31), The third focusing mirror (32) and the CCD camera (33) are sequentially arranged on the reflected light path of the second movable reflecting mirror (12). 8. A high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system according to claim 1, wherein the spectrum acquisition system comprises a CCD spectrometer (28), a first data transmission line (29), a first A computer (30), wherein the CCD spectrometer (28) is respectively connected to the first circularly polarized fluorescence collection module and the second circularly polarized fluorescence collection module through a Y-shaped optical fiber. 9. a kind of high-efficiency dual-channel circularly polarized fluorescence spectroscopy measurement system according to claim 1, is characterized in that, described Y-type optical fiber comprises Y-type optical fiber input end one (18), Y-type optical fiber input fiber one (19) ), Y-type fiber input end two (23), Y-type fiber input fiber two (24), Y-type fiber input end fusion splicing point (25), Y-type fiber output fiber (26), Y-type fiber output end ( 27), wherein the circularly polarized fluorescence acquisition module one is connected to the Y-type optical fiber input end fusion point (25) through the Y-type optical fiber input end one (18) and the Y-type optical fiber input fiber one (19) in turn; the circularly polarized fluorescence acquisition module 2. Connect the Y-type optical fiber input end fusion point (25) through the Y-type optical fiber input end two (23) and the Y-type optical fiber input optical fiber two (24) in turn, and the Y-type optical fiber input end fusion point (25) sequentially passes through the Y-type optical fiber. The output optical fiber (26) and the Y-type optical fiber output end (27) are connected to the spectrum acquisition system. 10. A high-efficiency dual-pass circularly polarized fluorescence spectrum measurement system according to claim 1, wherein the measured sample (10) is a spin-coated solid sample, a diamond-to-anvil high-voltage module, a One of the samples at room temperature and ambient temperature.

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