CN216259216U - One-step chitosan oligosaccharide extraction device - Google Patents
One-step chitosan oligosaccharide extraction device Download PDFInfo
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- CN216259216U CN216259216U CN202122488310.0U CN202122488310U CN216259216U CN 216259216 U CN216259216 U CN 216259216U CN 202122488310 U CN202122488310 U CN 202122488310U CN 216259216 U CN216259216 U CN 216259216U
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Abstract
The utility model discloses a one-step method chitosan oligosaccharide extraction device, wherein a material tank and a water tank are arranged at the front end of a stirrer, and a discharge hole is formed at the rear end of the stirrer; discharge gate department is equipped with the transmission band, be equipped with microwave heater on the transmission band, discharge gate below is located to transmission band one end, the other end is connected with the import of reaction box, the reaction box passes through pipe connection in purification case, purification case is connected in agitating unit, the external suction filtration case of agitating unit, suction filtration case passes through pipe connection in microwave drying device to be equipped with centrifugal device on the pipeline between the two, microwave drying device's discharge gate department is equipped with the mesh sieve reducing mechanism. According to the utility model, the chitosan oligosaccharide molecules are subjected to electrophoresis in the gel through the purification box, so that the separation and purification are accelerated, the waste is reduced, the purity is higher, the process is simpler by adopting a flow process, and the preparation is carried out in a physical microwave degradation mode, so that the preparation efficiency is higher; the method has the characteristics of simple process, high extraction efficiency and high extraction purity.
Description
Technical Field
The utility model relates to the technical field of chitosan oligosaccharide extraction, in particular to a one-step chitosan oligosaccharide extraction device.
Background
Chitosan oligosaccharide is an upgrading product of chitin and chitosan products, is an oligosaccharide product with the polymerization degree of 2-20 obtained by degrading chitosan through a special biological enzyme technology, has the molecular weight of less than or equal to 3200Da, is a low molecular weight product with better water solubility, large functional action and high biological activity, is always an important field of application research of chitosan industry, has important research significance for the research of the bioactive structure-activity mechanism and the industrialized production of the chitosan oligosaccharide, the Niao chromatography is one of the most effective methods for separating the chitosan oligosaccharide, the main steps are that the solution to be purified is put on a gel column, then, pure water is used for elution, small-molecular chitosan oligosaccharide passes through the particles of the Ninito, and large-molecular impurities are excluded by gel particles.
At present, the preparation method of chitosan oligosaccharide mainly comprises a chemical degradation method and an enzymatic degradation method.
The chemical degradation method is that high molecular weight chitosan is added into a reactor, hydrochloric acid is added according to a corresponding proportion, the mixture is stirred, the concentration of the chitosan is controlled, enzyme reagent is added, the pH value of the reaction mixture is controlled within a certain range by the hydrochloric acid, the reaction is kept for 2 to 24 hours, the reaction is complete, and then chitosan oligosaccharide hydrochloride is obtained by filtration and purification.
The enzymolysis method achieves the purpose of degradation by utilizing the mode that certain enzymes are specifically cracked and the molecular structure is changed, the process comprises the processes of solidification, activation, acid washing, enzymolysis, precipitation, fractionation, washing, drying and the like, and no other reaction by-products are generated in the whole degradation process, so that the method becomes a method which is more versatile in production at present and has the defects that: complex working procedure, low degradation efficiency, long time, large investment, large energy consumption, high product cost and incapability of completely eliminating the problem of environmental pollution.
Therefore, it is an urgent need to solve the problem of providing an extraction apparatus having a simple process, high extraction efficiency, and high extraction purity.
SUMMERY OF THE UTILITY MODEL
In view of the above, the utility model provides a one-step method chitosan oligosaccharide extraction device which has simple process, high extraction efficiency and high extraction purity.
In order to achieve the above object, the present invention provides the following technical solutions, including: the device comprises a stirrer, a microwave heater, a reaction box, a reaction kettle, a suction filtration box and a microwave drying device; wherein, the front end of the stirrer is provided with a material tank and a water tank, and the rear end is provided with a discharge hole; discharge gate department is equipped with the transmission band, be equipped with microwave heater on the transmission band, discharge gate below is located to transmission band one end, and the other end is connected with the import of reaction box, the reaction box passes through pipe connection in purification case, purification case connection is in agitating unit, the external suction filtration case of agitating unit, suction filtration case passes through pipe connection in microwave drying device to be equipped with centrifugal device on the pipeline between the two, microwave drying device's discharge gate department is equipped with the mesh sieve reducing mechanism.
Preferably, in the above one-step method chitosan oligosaccharide extraction apparatus, a control valve is provided on a connecting pipeline between the material tank and the stirrer.
Preferably, in the above one-step chitosan oligosaccharide extraction device, the purification tank comprises: the device comprises a purification tank, a positive electrode socket, a negative electrode socket and a separation membrane; purification tank deck portion is equipped with the inlet pipe, and the bottom is equipped with the discharging pipe, and jar internal portion is equipped with positive electrode socket, negative electrode socket top is located to positive electrode socket, the separation membrane is located between positive electrode socket, the negative electrode socket, and gomphosis is on the purification jar.
Preferably, in the one-step chitosan oligosaccharide extraction device, the reaction between the chitosan oligosaccharide and alcohol and hydrogen peroxide is performed in the reaction tank.
According to the technical scheme, compared with the prior art, the utility model discloses the one-step chitosan oligosaccharide extraction device, chitosan oligosaccharide molecules are subjected to electrophoresis in gel through the purification box, the separation and purification are accelerated, the waste is reduced, the purity is higher, the process is simpler by adopting a process, and the preparation is carried out in a physical microwave degradation mode, so that the preparation efficiency is higher; the method has the characteristics of simple process, high extraction efficiency and high extraction purity.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of the present invention.
FIG. 2 is a schematic view of the structure of the purification box of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Please refer to fig. 1-2, which is a device for extracting chitosan oligosaccharide by one-step method disclosed in the present invention.
The utility model comprises the following steps: the device comprises a stirrer 1, a microwave heater 3, a reaction box 4, a reaction kettle, a suction filtration box 7 and a microwave drying device 9; wherein, the front end of the stirrer 1 is provided with a material tank 11 and a water tank 12, and the rear end is provided with a discharge hole; discharge gate department is equipped with transmission band 2, be equipped with microwave applicator 3 on transmission band 2, the discharge gate below is located to 2 one end of transmission band, and the other end is connected with the import of reaction box 4, reaction box 4 passes through pipe connection in purification case 5, purification case 5 is connected in agitating unit 6, 6 external suction box 7 of agitating unit, suction box 7 passes through pipe connection in microwave drying device 9 to be equipped with centrifugal device 8 on the pipeline between the two, the discharge gate department of microwave drying device 9 is equipped with sieve reducing mechanism 10.
In order to further optimize the technical scheme, control valves are arranged on connecting pipelines between the material tank 11 and the water tank 12 and the stirrer 1.
In order to further optimize the above solution, the purification tank 5 comprises: a purification tank 51, a positive electrode jack 55, a negative electrode jack 54, and a separation membrane 56; purification jar 51 top is equipped with inlet pipe 52, and the bottom is equipped with discharging pipe 53, and internal portion of jar is equipped with anodal electrode socket 55, negative pole electrode socket 54, anodal electrode socket 55 is located negative pole electrode socket 54 top, separation membrane 56 is located between anodal electrode socket 5, the negative pole electrode socket 54, and the gomphosis is on purification jar 51.
In order to further optimize the technical scheme, alcohol and hydrogen peroxide are reacted with the chitosan oligosaccharide in the reaction box 4.
In order to further optimize the technical scheme, when the device is used, the material tank 11 and the water tank 12 are opened by controlling the control valve, materials are conveyed to the stirrer 1 according to a certain proportion and are uniformly mixed by the stirrer 1, the uniformly mixed materials flow through the microwave heater 3 through the conveying belt 2 and are subjected to microwave radiation by the microwave heater 3, then a high molecular chitosan solution is degraded to form a low molecular weight chitosan solution, a low molecular weight chitosan feed liquid is conveyed to the reaction box 4 through the conveying belt 2 to perform chemical reaction, so that the concentration of the chitosan solution is reduced and fully dissolved, the chitosan solution is subjected to chemical reaction of alcohol and hydrogen peroxide and is precipitated to form a low molecular weight chitosan oligosaccharide mixture solution, then the chitosan solution is subjected to purification reaction by the purification box 5, positive and negative electrodes are additionally arranged at the upper and lower ends of the purification tank, the electrodes are electrified, and chitosan oligosaccharide molecules are subjected to electrophoresis in gel, the separation and purification are accelerated, the yield is improved, the chitosan oligosaccharide mixture solution with low molecular weight is fully dissolved by uniformly stirring through the soaking and stirring device 6, the alcohol and hydrogen peroxide are pumped out through the suction filtration pipe to form chitosan oligosaccharide with certain viscosity, the chitosan oligosaccharide is dried by the centrifugal device 8 to form blocky chitosan oligosaccharide, and the blocky chitosan oligosaccharide is dried by the microwave drying device 9, crushed and screened through the sieve crushing device 10 to form solid granular chitosan oligosaccharide with low molecular weight and easy solubility in water.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (3)
1. A one-step method chitosan oligosaccharide extraction device is characterized by comprising the following steps: the device comprises a stirrer, a microwave heater, a reaction box, a reaction kettle, a suction filtration box and a microwave drying device; wherein, the front end of the stirrer is provided with a material tank and a water tank, and the rear end is provided with a discharge hole; discharge gate department is equipped with the transmission band, be equipped with microwave heater on the transmission band, discharge gate below is located to transmission band one end, and the other end is connected with the import of reaction box, the reaction box passes through pipe connection in purification case, purification case connection is in agitating unit, the external suction filtration case of agitating unit, suction filtration case passes through pipe connection in microwave drying device to be equipped with centrifugal device on the pipeline between the two, microwave drying device's discharge gate department is equipped with the mesh sieve reducing mechanism.
2. The one-step method for extracting chitooligosaccharide extracting device according to claim 1, wherein the connecting pipeline between the material tank and the water tank and the stirrer is provided with a control valve.
3. The one-step method chitosan oligosaccharide extraction device of claim 1, wherein said purification box comprises: the device comprises a purification tank, a positive electrode socket, a negative electrode socket and a separation membrane; purification tank deck portion is equipped with the inlet pipe, and the bottom is equipped with the discharging pipe, and jar internal portion is equipped with positive electrode socket, negative electrode socket top is located to positive electrode socket, the separation membrane is located between positive electrode socket, the negative electrode socket, and gomphosis is on the purification jar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122488310.0U CN216259216U (en) | 2021-10-15 | 2021-10-15 | One-step chitosan oligosaccharide extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122488310.0U CN216259216U (en) | 2021-10-15 | 2021-10-15 | One-step chitosan oligosaccharide extraction device |
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| Publication Number | Publication Date |
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| CN216259216U true CN216259216U (en) | 2022-04-12 |
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| CN202122488310.0U Active CN216259216U (en) | 2021-10-15 | 2021-10-15 | One-step chitosan oligosaccharide extraction device |
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| CN (1) | CN216259216U (en) |
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2021
- 2021-10-15 CN CN202122488310.0U patent/CN216259216U/en active Active
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