CN216082487U - Low-cost fluorescence detection sensor - Google Patents
Low-cost fluorescence detection sensor Download PDFInfo
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- CN216082487U CN216082487U CN202122183712.XU CN202122183712U CN216082487U CN 216082487 U CN216082487 U CN 216082487U CN 202122183712 U CN202122183712 U CN 202122183712U CN 216082487 U CN216082487 U CN 216082487U
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Abstract
The utility model discloses a low-cost fluorescence detection sensor which comprises a main body, a side cover, shading cotton, an excitation light path device and a detection light path device, wherein the excitation light path device and the detection light path device are arranged on the main body; the detection light path device comprises a dustproof window sheet, a coupling lens, a second optical filter, a receiving lens, a photoelectric detector and a circuit board which are sequentially arranged from top to bottom; the side cover is connected with the main body through screws, the shading cotton is arranged on the inner side of the side cover, the light source plate is connected with the main body through screws, and the photoelectric detector is arranged on the circuit board; the fluorescence detection sensor has small volume, compact structure and easy operation; and the main structure can be formed by injection molding or CNC machining, so that the production cost is fully reduced, and the mass production is facilitated.
Description
Technical Field
The utility model belongs to the technical field of sensors, and particularly relates to a low-cost fluorescence detection sensor.
Background
In recent years, with the rapid development of related technologies such as biotechnology, microfluidics technology, artificial intelligence, and the like, the demand for fluorescence-induced detection technology in fields such as nucleic acid PCR detection, protein analysis, medical analysis, analytical chemistry, food safety, water quality analysis, environmental detection, and the like has been rising.
The induced fluorescence method is a method for exciting a fluorescent substance in a sample by incident light to emit fluorescence and performing photoelectric conversion by a photoelectric detector, and has the advantages of high detection sensitivity, simplicity in operation and the like. The new generation of analysis and test instrument will be more miniaturized, portable and automatic.
At present, in the fluorescent detection technology in the market, the stray light extinction processing of a detection instrument is poor, the signal noise is high, the size is large, the structure is complex, the assembly difficulty is high, the maintenance and the repair are inconvenient, the price is high, and the requirement of rapid field test is difficult to meet. To this end, we propose a low-cost fluorescence detection sensor to solve the above-mentioned problems in the background art.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a low-cost fluorescence detection sensor, which solves the above-mentioned problems of the prior art.
In order to achieve the purpose, the utility model provides the following technical scheme: a low-cost fluorescence detection sensor comprises a main body, a side cover, shading cotton, an excitation light path device and a detection light path device, wherein the excitation light path device and the detection light path device are arranged on the main body, and the excitation light path device comprises a light source plate, a collimating mirror, a light homogenizing plate, a first light filter, a dichroic mirror and an extinction triangular cone, wherein the light source plate, the collimating mirror, the light homogenizing plate, the first light filter, the dichroic mirror and the extinction triangular cone form an angle of 45 degrees with the light beam direction;
the detection light path device comprises a dustproof window sheet, a coupling lens, a second optical filter, a receiving lens, a photoelectric detector and a circuit board which are sequentially arranged from top to bottom;
the side cover is connected with the main body through screws, the shading cotton is arranged on the inner side of the side cover, the light source plate is connected with the main body through screws, and the photoelectric detector is arranged on the circuit board;
the main body and the side cover are both formed by injection molding or CNC machining.
The photoelectric detector is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM photoelectric tube or a CMOS detector.
The light source of the light source plate is an LED light source, laser or halogen tungsten bulb.
A manufacturing method of a low-cost fluorescence detection sensor specifically comprises the following steps:
step 1: a collimating lens in the excitation light path device is glued inside the main body, a light source plate is connected on the main body through screws, and a light homogenizing plate, a first light filter and a dichroic mirror forming an angle with the light beam direction are sequentially inserted into the main body;
step 2: a coupling lens in the detection light path device is glued in the main body, a dustproof window sheet is glued on the main body, a second optical filter is inserted into the main body, shading cotton is glued on a side cover, and then the side cover is connected on the main body through a screw;
and step 3: the receiving lens is glued inside the main body, and the circuit board is connected below the main body through screws.
Compared with the prior art, the utility model has the beneficial effects that: the low-cost fluorescence detection sensor integrates excitation light, light beam collimation, dodging, extinction, fluorescence collection, fluorescence detection and signal filtering, and is small in size, compact in structure and easy to operate; and the main structure can be formed by injection molding or CNC machining, so that the production cost is fully reduced, and the mass production is facilitated.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an exploded view of the present invention;
fig. 3 is a functional block diagram of the circuitry on the circuit board of the present invention.
In the figure: 1. a main body; 101. an extinction triangular cone; 2. a side cover; 201. shading cotton; 3. a light source plate; 4. a collimating mirror; 5. light homogenizing; 6. a first optical filter; 7. a dichroic mirror; 8. a dust-proof window sheet; 9. a coupling lens; 10. a second optical filter; 11. a receiving lens; 12. a photodetector; 13. a circuit board; 14. and (5) testing the sample to be tested.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model provides a low-cost fluorescence detection sensor as shown in figures 1-2, which comprises a main body 1, a side cover 2, shading cotton 201, an excitation light path device and a detection light path device, wherein the excitation light path device and the detection light path device are arranged on the main body 1, and the excitation light path device comprises a light source plate 3, a collimating mirror 4, a light homogenizing sheet 5, a first light filter 6, a dichroic mirror 7 forming an angle of 45 degrees with the light beam direction and an extinction triangular cone 101 which are sequentially arranged from left to right;
the detection light path device comprises a dustproof window sheet 8, a coupling lens 9, a second optical filter 10, a receiving lens 11, a photoelectric detector 12 and a circuit board 13 which are sequentially arranged from top to bottom;
the side cover 2 is connected with the main body 1 through screws, the shading cotton 201 is arranged on the inner side of the side cover 2, the light source plate 3 is connected with the main body 1 through screws, and the photoelectric detector 12 is arranged on the circuit board 13;
the main body 1 and the side cover 2 are both injection molded or CNC machined.
The light source of the light source plate 3 is an LED light source, laser or halogen tungsten bulb.
A manufacturing method of a low-cost fluorescence detection sensor specifically comprises the following steps:
step 1: a collimating lens 4 in the excitation light path device is glued inside the main body 1, a light source plate 3 is connected on the main body 1 through screws, and a light homogenizing sheet 5, a first optical filter 6 and a dichroic mirror 7 forming an angle of 45 degrees with the light beam direction are sequentially inserted into the main body 1;
step 2: a coupling lens 9 in the detection light path device is glued inside the main body 1, a dustproof window 8 is glued on the main body 1, a second optical filter 10 is inserted into the main body 1, a shading cotton 201 is glued on the side cover 2, and then the side cover 2 is connected on the main body 1 through screws;
and step 3: the receiving lens 11 is glued inside the body 1, and the circuit board 13 is screwed below the body 1.
Excitation light emitted by a light source of the light source plate 3 passes through the collimating lens 4, the light homogenizing sheet 5 and the first optical filter 6 to obtain uniformly collimated monochromatic excitation light, then passes through the dichroic mirror 7 forming an angle of 45 degrees with the light beam direction to obtain upward excitation light, and then reaches a sample to be measured 14 through the coupling lens 9 and the dustproof window sheet 8.
The sample 14 to be detected emits fluorescence under the action of the exciting light, and the fluorescence passes through the dustproof window 8 and the coupling lens 9, then passes through the dichroic mirror 7, the second optical filter 10 and the receiving lens 11 to reach the photoelectric detector 12, and then is subjected to signal processing through the circuit board 13.
Referring to fig. 3, the circuit board 13 is provided with a transimpedance amplifier circuit, a low-pass filter circuit, a secondary amplifier circuit, and a circuit interface. The output end of the photoelectric detector 12 is connected to the input end of the transimpedance amplification circuit, the output end of the transimpedance amplification circuit is connected to the input end of the low-pass filter circuit, the output end of the low-pass filter circuit is connected to the input end of the second-stage amplification circuit, and the output end of the second-stage amplification circuit is connected to the circuit interface.
Compared with the prior art, the utility model has the advantages of low cost, small volume, compact structure and strong universality.
The fluorescence detection sensor integrates excitation light, light beam collimation, dodging, extinction, fluorescence collection, fluorescence detection, filtering and signal shielding protection, and has the advantages of small volume, compact structure and easy operation;
an extinction triangular cone 101 is arranged in a main body 1 of the fluorescence detection sensor, so that stray light of an excitation beam and ambient stray light can be eliminated fully;
the fluorescence detection sensor has a compact structure, is beneficial to injection molding or CNC machining, and fully reduces the production cost;
the structure of the fluorescence detection sensor is modularized, and batch production, graded quality inspection and maintenance are facilitated.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the utility model.
Claims (6)
1. The utility model provides a low-cost fluorescence detection sensor, includes main part (1), side cap (2), shading cotton (201), the excitation light path device and the detection light path device that set up on main part (1), its characterized in that: the excitation light path device comprises a light source plate (3), a collimating mirror (4), a light homogenizing plate (5), a first optical filter (6), a dichroic mirror (7) forming an angle of 45 degrees with the light beam direction and an extinction triangular cone (101), which are sequentially arranged from left to right horizontally;
the detection light path device comprises a dustproof window sheet (8), a coupling lens (9), a second optical filter (10), a receiving lens (11), a photoelectric detector (12) and a circuit board (13), which are sequentially arranged from top to bottom;
the side cover (2) is connected with the main body (1) through screws, the shading cotton (201) is arranged on the inner side of the side cover (2), the light source board (3) is connected with the main body (1) through screws, and the photoelectric detector (12) is arranged on the circuit board (13).
2. A low cost fluorescence detection sensor according to claim 1, wherein: the main body (1) and the side cover (2) are both formed by injection molding or CNC machining.
3. A low cost fluorescence detection sensor according to claim 1, wherein: the photoelectric detector (12) is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM photoelectric tube or a CMOS detector.
4. A low cost fluorescence detection sensor according to claim 1, wherein: the light source of the light source plate (3) is an LED light source.
5. A low cost fluorescence detection sensor according to claim 1, wherein: the light source of the light source plate (3) is laser.
6. A low cost fluorescence detection sensor according to claim 1, wherein: the light source of the light source plate (3) is a halogen tungsten bulb.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122183712.XU CN216082487U (en) | 2021-09-10 | 2021-09-10 | Low-cost fluorescence detection sensor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122183712.XU CN216082487U (en) | 2021-09-10 | 2021-09-10 | Low-cost fluorescence detection sensor |
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| Publication Number | Publication Date |
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| CN216082487U true CN216082487U (en) | 2022-03-18 |
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| CN202122183712.XU Active CN216082487U (en) | 2021-09-10 | 2021-09-10 | Low-cost fluorescence detection sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113655043A (en) * | 2021-09-10 | 2021-11-16 | 波粒光子(苏州)精密光电科技有限公司 | Low-cost fluorescence detection sensor and manufacturing method thereof |
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2021
- 2021-09-10 CN CN202122183712.XU patent/CN216082487U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113655043A (en) * | 2021-09-10 | 2021-11-16 | 波粒光子(苏州)精密光电科技有限公司 | Low-cost fluorescence detection sensor and manufacturing method thereof |
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