CN215828803U - A simple and portable cryopreserved cell recovery box - Google Patents
A simple and portable cryopreserved cell recovery box Download PDFInfo
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- CN215828803U CN215828803U CN202121419348.6U CN202121419348U CN215828803U CN 215828803 U CN215828803 U CN 215828803U CN 202121419348 U CN202121419348 U CN 202121419348U CN 215828803 U CN215828803 U CN 215828803U
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Abstract
本实用新型涉及一种新型冻存细胞复苏盒，包括冻存盒外壳，隔热保温板，可充电移动电池，电量显示屏，密封盒盖，手柄，加热管，水浴区，温度传感器，温度显示屏，温度指示灯，操作面板，电池联动开关，旋转冻存管架，管口弹性橡胶，旋转冻存管架转速调节按钮，水面刻度线，时间显示屏。该细胞复苏盒结构简单、便于携带、使用方便、成本低廉；既能进行精准温度调控，又能通过旋转冻存管架促进冻存细胞的快速融化，提高细胞存活率。可同时复苏多种不同规格冻存管的细胞，既提升实验效率，又节省实验时间，为进行细胞复苏的实验操作人员带来极大便利。
The utility model relates to a novel cryopreservation cell recovery box, comprising a cryopreservation box shell, a heat insulation board, a rechargeable mobile battery, a power display screen, a sealed box cover, a handle, a heating tube, a water bath area, a temperature sensor, and a temperature display. Screen, temperature indicator light, operation panel, battery linkage switch, rotary freezing tube rack, elastic rubber nozzle, rotary freezing tube rack speed adjustment button, water surface scale line, time display. The cell recovery box is simple in structure, easy to carry, easy to use, and low in cost; it can not only perform precise temperature control, but also promote the rapid thawing of cryopreserved cells by rotating the cryopreservation tube rack, thereby improving the cell survival rate. Cells in cryopreservation tubes of different specifications can be recovered at the same time, which not only improves the experimental efficiency, but also saves the experimental time, which brings great convenience to the experimental operators who carry out cell recovery.
Description
Technical Field
The utility model relates to the technical field of recovery of cryopreserved cells, in particular to a simple and portable recovery box for cryopreserved cells.
Background
Cell recovery refers to a process of thawing cells frozen in liquid nitrogen or in a freezer at 80 ℃ below zero, then culturing again, and restoring the cells to growth. Cell resuscitation has an important influence on cell viability, and different cell viability can influence the subsequent growth state of cells, further influence the sensitivity of the cells to drugs, and have an important influence on multiple fields such as life sciences, drug research and development, food sciences and the like.
At present, no simple portable device special for cell recovery exists in the market, warm water at 37 ℃ is prepared in a beaker for cell recovery in a laboratory, and laboratory personnel need to take out cells from a cell freezing area, fix the cells on a buoy or hold a freezing tube in the beaker by hand, melt the cells, centrifugally take out sediments in an aseptic operation room, and uniformly mix the sediments in a cell culture medium for culture. The whole resuscitation process has the following problems: 1) a common liquid nitrogen tank or a refrigerator at 80 ℃ below zero is usually adopted in part of laboratories and is intensively placed in a common laboratory area, the time taken between taking out cells is not fixed after warm water at 37 ℃ is prepared in the laboratory, and the beaker is not provided with a cup cover and any heat preservation device, is greatly influenced by the external temperature, particularly the water temperature in winter can be quickly reduced, so that the required melting time of the cells is prolonged, and the cell activity is reduced. 2) Some labs try to adopt the water bath to control temperature, but the water bath is generally great in size and weight, and the required time of rising temperature is longer, and need insert the electricity at any time and use, is difficult to remove it to the cell cryopreserving region and waits for in time to carry out the water bath. 3) Some experimenters shake through handheld cryopreserved pipe and unfreeze it fast, because liquid nitrogen temperature is extremely low about 196 ℃ below zero, the sealing membrane is very easily peeled off, the process of shaking probably leads to recovery water or the bacterium on hand to get into cryopreserved pipe, increases the contaminated risk of cell. Therefore, aiming at the practical problems faced by the existing cell recovery process, a novel simple portable cryopreservation cell recovery box is urgently needed to be designed.
Disclosure of Invention
The utility model aims to provide a novel laboratory cryopreserved cell resuscitation box which can accurately control temperature, is simple to operate and is convenient to carry.
In order to solve the above problems, the specific technical scheme of the utility model is as follows: a simple and portable cryopreservation cell recovery box is characterized in that a rotary cryopreservation pipe frame is arranged in the cell recovery box, a heating pipe is arranged below the bottom of the cell recovery box, a rechargeable mobile battery is arranged below the bottom of the cell recovery box, a sealing box cover is arranged at the upper part of the cell recovery box, an operation panel is arranged at the front part of the cell recovery box, and a temperature display screen, a temperature indicator lamp, a battery linkage switch, an electric quantity display screen and a rotary cryopreservation pipe frame rotating speed adjusting button are arranged on the operation panel;
the rotatory pipe support of depositing that freezes deposits the pipe support by freezing and deposits a tub fixed plate, freezes and deposits a tub fixed plate support, and support fixing device constitutes, freezes to deposit and is equipped with a plurality of test tube hole on the tub fixed plate.
Foretell simple and easy portable cryopreserved cell box, cell come back to life and be equipped with thermal-insulated heated board in the box outer wall.
The simple and portable cryopreserved cell resuscitation box is characterized in that the inner ring of the test tube hole is provided with tube orifice elastic rubber.
The simple and portable cryopreserved cell recovery box is characterized in that the inner wall of the cell recovery box is provided with water surface scale marks and a temperature sensor.
Foretell simple and easy portable cryopreserved cell recovery box, cell recovery box outer wall is equipped with the time display screen.
The simple and portable cryopreservation cell resuscitation box is characterized in that a handle is arranged above the sealing box cover.
The simple and portable cryopreserved cell recovery box can be further connected with an external power supply.
Novel cryopreserved cell resuscitating box inside contains intensification temperature regulating device, according to cryopreserved cell resuscitating requirement, heats water, can pull out the power after reaching the formulation temperature, removes at will, continues the power supply through built-in portable battery this moment, makes the heating pipe continue work and makes it keep constant temperature.
The simple portable frozen cell recovery box is not only connected with a working power supply, but also internally provided with a rechargeable mobile battery, after water is heated to 37 ℃, the recovery box can be moved at any time, and at the moment, the power is continuously supplied through the internal mobile battery, so that the heating pipe continuously works to achieve the purpose of constant temperature.
The simple portable cryopreservation cell resuscitation box is externally connected with an operation panel, displays set temperature and real-time temperature, and is in a heating or heat preservation state through an indicator lamp.
The simple portable cryopreservation pipe frame in the cryopreservation cell resuscitation box is made of elastic colloid materials around the cryopreservation pipe, can tightly fix the cryopreservation pipes with various specifications, and prevents water from splashing into the pipe orifice.
The top end of the simple portable cryopreserved cell recovery box is provided with the sealable box cover, and after the cryopreserved cells are placed in the cell recovery box, the box cover is sealed, so that the recovery box can be kept in a constant temperature state.
Simple and easy portable cryopreserved cell recovers the box and can be according to the experiment needs, recovers multicell cryopreserved cell simultaneously, holds cell recovery time according to the time display screen accuracy.
The simple and portable cell thawing box with the freezing storage pipe frame replaced can be used for a long time, and resources are fully utilized.
The utility model meets but is not limited to cell recovery, when a water bath device with other temperature is needed in an experiment, the temperature can be adjusted, and the rotary cryopreservation pipe frame is disassembled and replaced by pipe holes with other specifications, so that various experiment requirements are met.
The simple and portable cryopreserved cell resuscitation box is constant in temperature, simple and portable, convenient to use and low in cost.
The utility model has the following beneficial effects:
the recovery box is in a square shape, water can be heated up through an external power supply, then the box body can be moved at any time, power is continuously supplied through the internal mobile battery, and the purpose of keeping constant temperature by continuous work of the heating pipe is achieved. This novel cryopreserved cell resuscitating box can effectively solve when cryopreserved cell resuscitates, adverse effect that recovery water temperature change caused cell activity also enables water bath temperature through the design of rotatory cryopreserved pipe support and evenly, accelerates the cryopreserved cell to melt. Meanwhile, the inner ring of the cryopreservation pipe frame is designed by using elastic colloid, so that the cryopreservation pipes in different shapes can be fixed simultaneously, and the risk that resuscitation water enters the cryopreservation pipes along the pipe openings to increase cell contamination can be avoided. The cell thawing box for freezing preservation adopts a combined structure, has diversified integral functions, constant temperature, simple operation, convenient carrying and tight fixation, meets the thawing requirements of freezing preservation cells, and brings great convenience to cell thawing experimenters.
Drawings
FIG. 1 is a front view of a cryopreserved cell recovery cassette.
FIG. 2 is a sectional view of the structure of the cell thawing box for freezing.
FIG. 3 is a schematic view of a rotary cryopreservation tube rack.
FIG. 4 is the top view of the cover of the sealed box of the cell resuscitating box without cover.
The device comprises a freezing storage box shell, a heat insulation board, a chargeable mobile battery, an electric quantity display screen, a sealing box cover, a handle, a heating pipe, a water bath area, a temperature sensor, a temperature display screen, a temperature indicator lamp, an operation panel, a battery gang switch, a rotary freezing storage pipe frame, a freezing storage pipe fixing plate support, a support fixing device, an elastic rubber pipe orifice, a rotating speed adjusting button, a water surface scale mark, a time display screen, a rotating speed adjusting button, a water surface scale mark and a water surface scale mark, wherein the shell is 1, the heat insulation board is 2, the chargeable mobile battery is 3, the electric quantity display screen is 4, the sealing box cover is 5, the handle is 6, the heating pipe is 7, the water bath area is 8, the temperature sensor is 9, the temperature display screen is 10, the temperature indicator lamp is 11, the operation panel is 12, the battery gang switch is 13, the rotating speed adjusting button is 16, the rotating freezing storage pipe frame, the water surface scale mark is 14-1, the freezing storage pipe fixing plate support is 14-2, the support fixing device is a support fixing device, the elastic rubber pipe orifice elastic rubber is 15, the rotating speed adjusting button is 16, and the time display screen is 18.
Detailed Description
In order to better understand the inventive and practical solution, the design solution in this study will be described in detail and clearly with reference to the attached drawings. Fig. 1 and 2 are schematic structural diagrams of the utility model, and the cryopreservation cell resuscitation box not only meets the requirements of constant temperature and simplicity and portability, but also can be used for designing and fixing cryopreservation tubes of different specifications through elastic colloids at the inner ring of a cryopreservation tube frame. The method has the advantages of constant temperature, simple operation, portability and effective resuscitation.
Example 1
As shown in figures 1-4, a cell thawing box, a water bath area 8 is arranged in the cell thawing box 1, a rotary freezing pipe frame 14 is arranged in the cell thawing box 1, an operation panel 12 is provided with a rotary freezing pipe frame rotating speed adjusting button 16, and the rotary freezing pipe frame rotating speed adjusting button 16 is connected with the rotary freezing pipe frame 14 through a speed-adjustable direct current motor. The bottom in the cell recovery box 1 is provided with a heating pipe 7, a rechargeable mobile battery 3 is arranged below the bottom of the cell recovery box 1, the rechargeable mobile battery 3 is respectively connected with a battery gang switch 13 and an electric quantity display screen 4, the inner wall of the cell recovery box 1 is provided with a water surface scale mark 17 and a temperature sensor 9, and a temperature display screen 10 on an operation panel 12 is respectively connected with a temperature indicator lamp 11 and the temperature sensor 9. The upper part of the cell recovery box 1 is provided with a sealing box cover 5, and a handle 6 is arranged above the sealing box cover 5.
The rotary cryopreservation pipe frame 14 consists of a cryopreservation pipe fixing plate 14-1, a cryopreservation pipe fixing plate bracket 14-2 and a bracket fixing device 14-3, wherein a plurality of test tube holes are formed in the cryopreservation pipe fixing plate 14-1. The inner ring of the test tube hole is provided with a tube opening elastic rubber 15. The outer wall of the cell recovery box 1 is internally provided with a heat insulation board 2. The outer wall of the cell recovery box 1 is provided with a time display screen 18.
As shown in figure 1, when cells are to be resuscitated, firstly, a proper amount of water is added into a water bath area 8, an external power supply is plugged, the temperature is set to be 37 ℃, heating is started, and when the temperature reaches the set temperature, the power supply can be pulled out. And (3) turning on the battery linkage switch 13, and continuously supplying power through the built-in rechargeable mobile battery 3 to ensure that the heating pipe 7 continuously works to keep constant temperature. At the moment, the frozen cell recovery box can be moved to a liquid nitrogen or a refrigerator at minus 80 ℃, and frozen cells are prepared to be taken for water bath recovery. Placing the frozen cells in a frozen cell recovery box, pressing a rotating speed adjusting button 16 of the rotary freezing storage tube rack to make the rotary freezing storage rack 14 rotate to assist the dissolution, and then tightly fastening a sealing box cover 5 to keepThe water temperature is constant. After the cells are placed in the frozen cell recovery box, timing by a time display screen, moving the frozen cell recovery box to quickly return to a sterile cell operating room, observing whether the frozen cells are completely thawed or not when the time is 1min, centrifuging after thawing, discarding the supernatant, taking the precipitate, uniformly mixing the precipitate in a cell culture medium, placing the cell culture medium in CO at 37 ℃ and 5 percent2And (5) culturing in an incubator, and completing the recovery work of the cryopreserved cells.
The utility model meets but is not limited to cell recovery, when other water bath temperatures and devices are needed in the experiment, the temperature can be set, the rotary cryopreservation pipe frame is detached and replaced by pipe holes with other specifications, such as the hole diameters of 1.5, 2, 4 and 10mL EP pipes, and various experiment requirements are met.
The embodiments described in this specification are merely exemplary of the inventive concept and the scope of the present invention should not be considered limited to the embodiments shown, but includes equivalent technical means according to the concept of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121419348.6U CN215828803U (en) | 2021-06-24 | 2021-06-24 | A simple and portable cryopreserved cell recovery box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121419348.6U CN215828803U (en) | 2021-06-24 | 2021-06-24 | A simple and portable cryopreserved cell recovery box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215828803U true CN215828803U (en) | 2022-02-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121419348.6U Active CN215828803U (en) | 2021-06-24 | 2021-06-24 | A simple and portable cryopreserved cell recovery box |
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| Country | Link |
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| CN (1) | CN215828803U (en) |
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2021
- 2021-06-24 CN CN202121419348.6U patent/CN215828803U/en active Active
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