CN215688729U - Double-balloon fixable animal perfusion needle - Google Patents
Double-balloon fixable animal perfusion needle Download PDFInfo
- Publication number
- CN215688729U CN215688729U CN202022994910.XU CN202022994910U CN215688729U CN 215688729 U CN215688729 U CN 215688729U CN 202022994910 U CN202022994910 U CN 202022994910U CN 215688729 U CN215688729 U CN 215688729U
- Authority
- CN
- China
- Prior art keywords
- balloon
- needle
- perfusion
- double
- fixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000010412 perfusion Effects 0.000 title claims abstract description 77
- 241001465754 Metazoa Species 0.000 title claims abstract description 25
- 239000007924 injection Substances 0.000 claims abstract description 45
- 238000002347 injection Methods 0.000 claims abstract description 45
- 239000011229 interlayer Substances 0.000 claims abstract description 38
- 238000002955 isolation Methods 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 8
- 229920001296 polysiloxane Polymers 0.000 claims description 5
- 239000013013 elastic material Substances 0.000 claims description 3
- 238000002224 dissection Methods 0.000 claims 1
- 210000005240 left ventricle Anatomy 0.000 abstract description 12
- 230000002861 ventricular Effects 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 210000005242 cardiac chamber Anatomy 0.000 abstract description 5
- 238000001467 acupuncture Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 14
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 210000004204 blood vessel Anatomy 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000002872 contrast media Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 210000005241 right ventricle Anatomy 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001308 heart ventricle Anatomy 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000005245 right atrium Anatomy 0.000 description 1
- 210000005077 saccule Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
本实用新型提供了一种双球囊可固定动物灌注针，包括套管，套管内设置有穿刺针，套管外设置有一双球囊固定夹层；双球囊固定夹层包括两个可膨胀扩大的球囊，用以进行膨胀扩大并固定灌注针；套管固定于设置有空腔的套管底座，穿刺针连接于穿刺针底座，穿刺针和穿刺针底座通过隔离塞固定在套管底座内；套管底座的侧壁上还连接有一固定注射装置，固定注射装置与双球囊固定夹层连通。本实用新型提供的双球囊可固定动物灌注针，通过在灌注针上设置双球囊固定夹层，使灌注针主体固定在小鼠心脏心室壁上，可有效避免现有技术中灌注针在灌注过程中脱落，灌注针头刺破左心室内壁进入其他心腔，以及针刺部位针孔变大后导致心内压力不足，灌注剂泄露等情况。
The utility model provides a double-balloon fixable animal perfusion needle, which comprises a cannula, a puncture needle is arranged in the cannula, and a double-balloon fixing interlayer is arranged outside the casing; the double-balloon fixing interlayer comprises two expandable and expandable The balloon is used to expand and fix the perfusion needle; the cannula is fixed on the cannula base provided with the cavity, the puncture needle is connected to the puncture needle base, and the puncture needle and the puncture needle base are fixed in the cannula base through the isolation plug; A fixed injection device is also connected to the side wall of the casing base, and the fixed injection device is communicated with the double-balloon fixed interlayer. The double-balloons provided by the utility model can fix the animal perfusion needle. By arranging the double-balloon fixing interlayer on the perfusion needle, the main body of the perfusion needle is fixed on the ventricular wall of the mouse heart, which can effectively avoid the perfusion needle in the prior art. During the process, the perfusion needle pierced the inner wall of the left ventricle and entered other cardiac chambers, and the needle hole at the acupuncture site became larger, resulting in insufficient intracardiac pressure and leakage of perfusate.
Description
Technical Field
The utility model relates to the technical field of perfusion needles, in particular to a double-balloon fixable animal perfusion needle.
Background
In the field of medical scientific research, animal tissue can be well fixed by heart perfusion of animal specimens, the lightest autolysis degree and the best morphological and tissue antigen retention degree are ensured, and meanwhile, a certain experimental basis is provided for quantitative protein analysis, nucleic acid molecule detection and morphological observation of the microstructure of the tissue. In the conventional perfusion, a common syringe needle is used for puncturing from the left ventricle, then physiological saline or corrosive liquid (formalin and the like) or blood vessel perfusion agent is injected into the syringe for perfusion fixation or perfusion development, the perfusion liquid enters into all levels of arteries after entering an aorta through the left ventricle, and then returns to the right atrium through all levels of veins through capillary vessels of the whole body. During the perfusion process, the perfusion needle is very easy to fall off due to the heart movement or puncture the inner wall of the left ventricle, so that the perfusion liquid flows into the right ventricle to cause perfusion failure. Meanwhile, in some animal experiments, such as animal blood vessel perfusion experiments, due to the fact that the skin near a puncture needle hole is hardened due to formalin perfusion, the puncture needle hole is enlarged, pressure is reduced when contrast medium is injected, and the contrast medium leaks to cause perfusion failure of a small blood vessel at the far end. And the traditional perfusion mode has low perfusion success rate, needs two people to operate simultaneously, can only operate 1-2 animals at a time, has long experiment time consumption and low efficiency.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides the double-balloon fixable animal perfusion needle which is convenient and practical, can fix the perfusion needle on the wall of the left ventricle and is convenient for heart perfusion.
The utility model adopts the following technical scheme for solving the technical problems:
the utility model provides a double-balloon fixable animal perfusion needle, which comprises a sleeve, wherein a puncture needle is arranged in the sleeve, and a double-balloon fixed interlayer is arranged outside the sleeve;
the double-balloon fixing interlayer comprises two inflatable and expandable balloons for inflation and expansion and fixing the perfusion needle;
the sleeve is fixed on a sleeve base provided with a cavity and communicated with the cavity, the puncture needle is connected with the puncture needle base, and the puncture needle base are fixed in the sleeve base through an isolation plug; an isolation plug disposed in the cavity
The side wall of the sleeve base is also connected with a fixed injection device which is communicated with the double-balloon fixed interlayer.
Further, the double-balloon fixing interlayer comprises a first balloon close to the tip of the puncture needle and a second balloon far away from the tip of the puncture needle; the first sacculus and the second sacculus on the double-sacculus fixing interlayer are made of elastic materials.
Furthermore, the second balloon is connected with the first balloon through a connecting part, and one end of the first balloon, which is far away from the second balloon, is communicated with the fixed injection device through the bottom of the interlayer; the connecting portion and the bottom portion of the interlayer are not deformable.
Further, the fixed injection device comprises an injection part, and the material of the injection part is selected from one of rubber and silica gel.
Further, the material of the isolation plug is selected from one of rubber and silica gel.
Further, the fixed injection device is connected with the perfusion needle through the injection tube and is communicated with the double-balloon fixed interlayer.
Further, a closing clip is arranged on the injection tube.
Further, the distance between the two balloons is 0.1-5 mm.
Furthermore, one side of the puncture needle base is provided with a holding part for taking the puncture needle base and the puncture needle out of the cannula base and the cannula.
Furthermore, the holding part is provided with anti-skid convex points.
By adopting the technical scheme, compared with the prior art, the utility model has the following technical effects:
the double-balloon fixable animal perfusion needle provided by the utility model has the advantages that the double-balloon fixable interlayer is arranged on the perfusion needle, after the perfusion needle is penetrated into the wall of the left ventricle of the heart of a mouse, air or liquid is injected into the double-balloon fixable interlayer, so that the two balloons positioned inside and outside the wall of the ventricle are expanded and enlarged, the main body of the perfusion needle is fixed on the wall of the heart ventricle of the heart of the mouse, and the problems that the perfusion needle is easy to fall off in the perfusion process, or the perfusion penetrates the inner wall of the left ventricle of a needle head to enter other heart chambers, the intracardiac pressure is insufficient after a needle hole of a needle punching part is enlarged, and the perfusion fails due to the leakage of a perfusion agent in the prior art can be effectively avoided. Greatly improving the success rate of animal experiment perfusion.
Drawings
FIG. 1 is a schematic diagram of a double-balloon fixable animal injection needle according to an embodiment of the utility model;
FIG. 2 is a schematic view of the assembly of a closure clip and syringe in a double-balloon fixable animal injection needle provided in accordance with an embodiment of the present invention;
FIG. 3 is a schematic structural view of a double-balloon fixable animal injection needle (when the balloon is inflated) provided by an embodiment of the utility model;
FIG. 4 is a schematic view of a double-balloon fixable animal infusion needle provided in accordance with an embodiment of the present invention penetrating a wall of a mouse ventricle;
FIG. 5 is a schematic diagram of balloon inflation after a double-balloon fixable animal injection needle provided by an embodiment of the utility model is inserted into a ventricular wall of a mouse;
FIG. 6 is a schematic view of a double-balloon fixable animal perfusion needle provided in accordance with an embodiment of the present invention, taken out of a puncture needle and its base after the needle has penetrated into a mouse ventricle wall;
wherein the reference numerals are: 1-sleeve, 2-puncture needle, 3-double-balloon fixing interlayer, 31-first balloon, 32-second balloon, 33-connecting part, 34-interlayer bottom, 4-sleeve base, 5-puncture needle base, 51-holding part, 6-isolating plug, 7-fixing injection device, 71-injection part, 72-sealing clip and 8-injection tube.
Detailed Description
The utility model provides a double-balloon fixable animal perfusion needle.
The following detailed description of the present invention is provided in connection with the accompanying drawings for better understanding of the present invention, but not to limit the scope of the present invention.
As shown in figure 1, the utility model provides a double-balloon fixable animal perfusion needle, which comprises a sleeve 1, wherein a puncture needle 2 is arranged in the sleeve 1, and a double-balloon fixed interlayer 3 is arranged outside the sleeve 1;
the double-balloon fixing interlayer 3 comprises two inflatable and expandable balloons for inflating and expanding and fixing the perfusion needle;
the double-balloon fixing interlayer 3 comprises a second balloon 32 close to the tip of the puncture needle 2 and a first balloon 31 far away from the tip of the puncture needle 2; the first balloon 31 and the second balloon 32 on the double-balloon fixing interlayer 3 are made of elastic materials. The second balloon 32 is connected with the first balloon 31 through a connecting part 33, and one end of the first balloon 31 far away from the second balloon 32 is communicated with the fixed injection device 7 through an interlayer bottom part 34; the connection portion 32 and the interlayer base 34 are not deformable. The first balloon 31 and the second balloon 32 in the double balloon fixing interlayer 3 can be inflated and enlarged by injecting gas or liquid into the double balloon fixing interlayer 3, and the shapes of the connecting part 32 and the interlayer bottom part 34 are kept unchanged.
The sleeve 1 is fixed on a sleeve base 4 provided with a cavity and is communicated with the cavity; the puncture needle 2 is fixed on a puncture needle base 5, and the puncture needle base 5 is fixed in a sleeve base 4 through an isolation plug 6; the isolating plug 6 is arranged in the cavity of the sleeve base 4; one side on the puncture needle base 5 is provided with a holding part 51 for taking the puncture needle base 5 and the puncture needle 2 out of the cannula base 4 and the cannula 1, and the holding part 51 is provided with anti-skid convex points, so that the taking out of the puncture needle base 5 and the puncture needle 2 is easier through the anti-skid convex points.
The material of the isolation plug 6 is selected from one of rubber and silicone. The puncture needle 2 can be taken out from the sleeve 1 and the isolating plug 6 through the puncture needle base 5, and the puncture needle base 5 is also taken out from the sleeve base 4, at the moment, the isolating plug 6 can be automatically closed after the puncture needle 2 is pulled out because the isolating plug 6 is made of rubber or silica gel. After the puncture needle 2 is pulled out, normal saline or perfusate can be injected into the sleeve 1 through the isolating plug 6, and the isolating plug can be automatically closed after the completion of primary perfusion, so that the normal saline or the perfusate is prevented from flowing out of the isolating plug.
The automatic recovery of the seal by pulling out the needle after the rubber or silicone septum 6 has been pierced by the needle is well known in the art and will not be described herein.
The side wall of the sleeve base 4 is also connected with a fixed injection device 7, and the fixed injection device 7 is communicated with the double-balloon fixed interlayer 3.
As shown in fig. 3, the fixed injection device 7 includes an injection part 71, and the material of the injection part 71 is selected from one of rubber and silicone. The fixed injection device 7 is connected with the perfusion needle through an injection tube 8 and is communicated with the double-sacculus fixing interlayer 3. Gas or liquid can be injected through the injection part 71, and the gas or liquid flows into the double-balloon fixing interlayer 3 through the injection pipe 8, so that the first balloon 31 and the second balloon 32 are expanded, and the perfusion needle is fixed on the ventricular wall of the mouse.
As shown in FIG. 2, a closure clip 72 is provided on the syringe 8. The injection part 71 is made of one of rubber and silica gel, after gas or liquid is injected through the injection part 71, an injection needle for injecting the gas or the liquid is pulled out, the injection part 71 can be automatically closed, at the moment, the injection tube 8 can be squeezed into a thin gap of the closing clamp 72, the closing clamp 72 can close the injection tube 8, and the double-balloon fixing interlayer 3 is prevented from leaking air by matching with the material of the injection part 71.
In a preferred embodiment of the utility model, the distance between the two balloons is 0.1-5 mm. Namely, the length of the connecting part 33 between the first balloon 31 and the first balloon 32 is 0.1-5 mm, the distance is selected according to the body type of the perfused mouse and the thickness of the ventricular wall of the left ventricle, and the perfusion needle can be fixed on the ventricular wall after the double-balloon fixing interlayer 3 is expanded.
As shown in fig. 4 to 6, a method for using a double-balloon fixable animal perfusion needle according to an embodiment of the present invention includes:
firstly, according to the heart perfusion and blood vessel perfusion modeling standard of a mouse, splitting the thoracic cavity of the mouse, exposing the apex of the heart, finding the position of a left ventricle, inserting a double-balloon perfusion needle from the left ventricle through a puncture needle 2 and a sleeve 1, and stopping needle insertion after breakthrough feeling occurs;
then, injecting gas or liquid into the double balloon fixing interlayer 3 through the injection part 71 of the fixing injection device 7 to expand the first balloon 31 and the second balloon 32, and fixing the perfusion needle body on the ventricular wall of the mouse through the squeezing action of the first balloon 31 and the second balloon 32;
then, the puncture needle 2 is pulled out through the pinching part 51 of the puncture needle base 5, the cannula 1 and the double-balloon fixed interlayer are left in the left ventricle, the isolating plug 6 is automatically closed at the moment, the injector is inserted from the isolating plug 6, physiological saline is injected into the left ventricle through the cavity of the cannula base 4 and the cannula 1, meanwhile, the opening of the right auricle of the heart of the mouse is formed, the flow rate of the infusion apparatus is adjusted to be consistent with the blood pumping speed of the heart at the moment, the effluent liquid of the right auricle of the heart of the mouse is clarified and bright, the tail and the four limbs become white and lose the blood color, and the infusion of the physiological saline is considered to be finished;
and finally, pulling out the normal saline injector, replacing corrosive fixing liquid such as formalin and the like, inserting the corrosive fixing liquid into the isolating plug 6 for perfusion, and keeping the perfusion needle on the wall of the heart chamber of the mouse to ensure the stability of the circulating pressure of the mouse body until the materials are obtained.
The heart puncture hole can be well plugged by the extrusion of the double saccules, so that the perfusion needle cannot fall off from the wall of the heart chamber in the perfusion process; the perfusion needle is fixed on the ventricular wall by the double balloons in the perfusion process, so that the perfusion failure caused by the fact that the perfusion needle is further inserted into the inner ventricular wall of the heart of the mouse to enter the right ventricle without paying attention in the perfusion process is avoided; and the extrusion of two sacculums makes the heart chamber maintain higher pressure all the time, is difficult to appear stationary liquid refluence or reveal from the puncture hole, makes animal experiment perfusion's success rate and efficiency increase substantially.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications or alterations to this practice will occur to those skilled in the art and are intended to be within the scope of this invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022994910.XU CN215688729U (en) | 2020-12-14 | 2020-12-14 | Double-balloon fixable animal perfusion needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022994910.XU CN215688729U (en) | 2020-12-14 | 2020-12-14 | Double-balloon fixable animal perfusion needle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215688729U true CN215688729U (en) | 2022-02-01 |
Family
ID=79984973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022994910.XU Active CN215688729U (en) | 2020-12-14 | 2020-12-14 | Double-balloon fixable animal perfusion needle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215688729U (en) |
-
2020
- 2020-12-14 CN CN202022994910.XU patent/CN215688729U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104770361B (en) | Supplied-liver quickly-fixed perfusion device for liver transplant | |
| CN103055409B (en) | Transthoracic puncture aorta intubation tube suitable for minimally invasive cardiac surgery | |
| WO2022156747A1 (en) | Deep vein puncture assembly and deep vein puncture needle kit | |
| CN215688729U (en) | Double-balloon fixable animal perfusion needle | |
| CN206777388U (en) | The arteriopuncture sleeve pipe of end of tape sacculus | |
| CN203777464U (en) | Percutaneous puncture minimally-invasive cardiac surgery balloon blocking, cardioplegic solution perfusing and exhausting cannula | |
| JPH02307480A (en) | Intravenous atrial septal defect hole closing apparatus | |
| CN110339435A (en) | A triple trocar device and method for deep tissue puncture | |
| CN205924174U (en) | General type puncture drainage package | |
| CN207186907U (en) | Drainage tube | |
| CN203507281U (en) | Closed chest puncture and drainage tube | |
| CN203075346U (en) | Infant intra-arterial infusion syringe needle | |
| CN109481070A (en) | A kind of implantable internal carotid constant speed injection device and its operating method | |
| CN203724593U (en) | Pleuroperitoneal cavity puncturing drainage tube capable of conducting ultrafiltration on proteins | |
| CN210962068U (en) | Intracardiac package puncture drawing liquid device for branch of academic or vocational study | |
| CN210277306U (en) | Trocar for treating cystic cirrhosis | |
| CN211434381U (en) | Anti-blocking thoracic drainage tube for quick puncture and intubation | |
| CN212308873U (en) | Medical simulation doll with built-in infusion port | |
| CN208274819U (en) | A kind of Novel urethral catheter | |
| CN211157863U (en) | A triple trocar device for deep tissue puncture | |
| CN211356032U (en) | Common carotid artery implantation device and implantable internal carotid artery constant-speed injection device | |
| CN201906251U (en) | Pericardicentesis indwelling drainage device | |
| CN203017040U (en) | Bayonet type central venipuncture injection syringe | |
| CN203043288U (en) | Transthoracic puncture aorta intubation tube suitable for minimally invasive cardiac surgery | |
| CN205107968U (en) | Rat jugular vein indwelling infusion device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |