CN215560280U

CN215560280U - Molecule detection consumable component

Info

Publication number: CN215560280U
Application number: CN202121438585.7U
Authority: CN
Inventors: 董贵兵; 陈婷婷
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-25
Filing date: 2021-06-25
Publication date: 2022-01-18
Anticipated expiration: 2031-06-25

Abstract

The utility model provides a molecule detection consumable component, which comprises a reaction tube, a sampling tube and a reagent tube, wherein the reaction tube is connected with the sampling tube; the top of the reaction tube is closed, the top surface is provided with a plurality of input ports, the top surface is fixedly provided with a plurality of connecting tubes corresponding to the input ports, the top of each input port is fixedly provided with a pointed cone, and each pointed cone is provided with a through hole communicated with the inside of the reaction tube; the bottom of the sampling tube is open and is blocked by the first film, the sampling tube is connected and matched with one of the connecting tubes, the sampling tube is pushed downwards, and the first film can be punctured by the pointed cone; the bottom of the reagent tube is opened and is blocked by a second thin film, the rest connecting tubes are connected with the reagent tube, the reagent tube is pushed downwards, and the second thin film can be punctured by a pointed cone; the device has the advantages of novel structure, simplicity in operation and convenience in use, the whole detection process is carried out in a sealed state, virus diffusion can be effectively prevented, and the detection accuracy and the detection safety are guaranteed.

Description

Molecule detection consumable component

Technical Field

The utility model relates to the field of detection consumables, in particular to a molecular detection consumable component.

Background

In the field of biomedical detection, the demand of molecular detection is increasingly expanding, for example, the detection of novel coronavirus nucleic acid is performed, a commonly used detection method is a fluorescent quantitative PCR method, the detection process is generally divided into two steps, nucleic acid amplification is performed firstly, then qualitative detection is performed, however, liquid transfer needs to be performed between the two steps, in the liquid transfer process, the problems of leakage or cross contamination of an amplification product may occur, the detection result is affected, and the safety needs to be improved.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model aims to provide a consumable component for molecular detection, which has the advantages of novel structure, simplicity in operation and convenience in use, the whole detection process is carried out in a sealed state, virus diffusion can be effectively prevented, and the detection accuracy and the detection safety are ensured.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a molecule detection consumable component, which comprises a reaction tube, a sampling tube and a reagent tube, wherein the reaction tube is connected with the sampling tube; the top of the reaction tube is closed, the top surface is provided with a plurality of input ports, the top surface is fixedly provided with a plurality of connecting tubes corresponding to the input ports, the top of each input port is fixedly provided with a pointed cone, and each pointed cone is provided with a through hole communicated with the inside of the reaction tube; the bottom of the sampling tube is open and is blocked by the first film, the sampling tube is connected and matched with one of the connecting tubes, the sampling tube is pushed downwards, and the first film can be punctured by the pointed cone; the bottom of the reagent tube is opened and is blocked by the second thin film, the rest connecting tubes are connected with the reagent tube, the reagent tube is pushed downwards, and the second thin film can be punctured by the pointed cone.

In a preferred technical scheme of the utility model, the pointed cone is of a thin-wall structure, the through holes are arranged on the side wall of the pointed cone, and the through holes are distributed in a circumferential array around the axis of the pointed cone.

In a preferred technical scheme of the utility model, the through hole extends along the inclined plane of the pointed cone, and the bottom end of the through hole is close to the bottom of the inclined plane of the pointed cone.

In a preferred technical scheme of the utility model, the sampling tube comprises a first tube body, the first film is arranged on the inner side of the bottom end of the first tube body, the cover body is arranged on the inner side of the top end of the first tube body, the first embedded grooves which are arranged along the radial direction are arranged on two sides of the opening at the top end of the first tube body, and the top surface of the cover body which is arranged is flush with the bottom of the first embedded groove.

In a preferred technical scheme of the utility model, the reagent tube comprises a second tube body, the top end of the second tube body is closed, the top surface of the second tube body is provided with a second embedded groove which is arranged along the radial direction, and the second film is arranged on the inner side of the bottom end of the second tube body.

In a preferred technical scheme of the utility model, the inner wall of the connecting pipe is provided with internal threads, the outer walls of the bottoms of the first pipe body and the second pipe body are provided with external threads, and the sampling pipe and the reagent pipe are connected and installed at the corresponding connecting pipe through threads.

In a preferred technical scheme of the utility model, the throwing port is of a hopper-shaped structure, and the diameter of the opening at the bottom end of the throwing port is smaller than that of the opening at the top end.

In a preferred technical scheme of the utility model, the reaction tube is of a bucket-shaped structure, and the bottom end of the reaction tube is blocked.

The utility model has the beneficial effects that:

the utility model provides a consumable component for molecular detection, which is novel in structure, wherein a sampling tube and a plurality of reagent tubes are arranged at the top of a reaction tube through connecting tubes, and when reaction is required, the sampling tube and the plurality of reagent tubes are sequentially rotated and pushed to puncture corresponding first thin films and second thin films, so that internal samples and reagents are subjected to mixed reaction; the whole detection process is carried out in a sealed state, virus diffusion can be effectively prevented, and the detection accuracy and the detection safety are guaranteed.

Drawings

FIG. 1 is a schematic structural diagram of a molecular assay consumable assembly according to an embodiment of the present invention;

FIG. 2 is a plan view of a reaction tube provided in an embodiment of the present invention;

FIG. 3 is a schematic diagram of a sampling tube provided in an embodiment of the present invention;

FIG. 4 is a top view of a coupon provided in an embodiment of the present invention;

FIG. 5 is a schematic diagram of the structure of a reagent vessel provided in an embodiment of the present invention;

FIG. 6 is a top view of a reagent tube provided in an embodiment of the present invention.

In the figure:

100. a reaction tube; 110. a throwing port; 120. taking over a pipe; 130. a pointed cone; 140. a through hole; 200 sampling tubes; 210. a film; 220. a first pipe body; 230. a cover body; 240. a first embedded groove; 300. a reagent tube; 310. a second film; 320. a second tube body; 330. a second embedded groove.

Detailed Description

The technical scheme of the utility model is further explained by the specific implementation mode in combination with the attached drawings.

As shown in fig. 1 to 6, the embodiment of the present invention discloses a consumable component for molecular assay, which includes a reaction tube 100, a sampling tube 200, and a reagent tube 300; the top of the reaction tube 100 is closed, the top surface is provided with a plurality of inlets 110, the top surface is fixedly provided with a plurality of connecting tubes 120 corresponding to the inlets 110, the top of the inlets 110 is fixedly provided with a pointed cone 130, and the pointed cone 130 is provided with a through hole 140 communicated with the interior of the reaction tube 100; the bottom of the sampling tube 200 is open and is sealed by the first film 210, the sampling tube 200 is connected and matched with one of the connecting tubes 120, the sampling tube 200 is pushed downwards, and the pointed cone 130 can puncture the first film 210; the bottom of the reagent tube 300 is open and sealed by the second film 310, the remaining adapter tubes 120 are connected with the reagent tube 300, and the reagent tube 300 is pushed downwards and the second film 310 can be punctured by the pointed cone 130.

The molecular detection consumable component is novel in structure, the sampling tube and the plurality of reagent tubes are arranged at the top of the reaction tube through the connecting tubes, and when a reaction is required, the sampling tube and the plurality of reagent tubes are sequentially rotated and pushed to puncture the corresponding first thin film and the second thin film, so that a sample and a medicament in the reaction tube are subjected to a mixed reaction; the whole operation is simple, the use is convenient, the whole detection process is carried out in a sealed state, the virus diffusion can be effectively prevented, and the detection accuracy and the detection safety are ensured; it should be noted that, the number of the reagent tubes is 3, and the reagent tubes are respectively used for storing the inhibiting solution, the lysis solution and the PCR reaction solution; more specifically, the first step is to advance the sampling tube, puncture the first membrane, and enter the sample solution in the sampling tube into the reaction tube; secondly, pushing a reagent tube with the inhibiting solution, and mixing and reacting the inhibiting solution with the sample solution after the inhibiting solution enters the reaction tube; a second step of forming a mixed solution A after the reaction is completed, and a third step of pushing a reagent tube with the lysate placed therein, wherein the lysate reacts with the mixed solution A after entering a reaction tube; and step four, a reagent tube for placing the PCR reaction solution is pushed, and the PCR reaction solution enters the reaction tube and then reacts with the B mixed solution.

Furthermore, the pointed cone 130 is a thin-walled structure, the through holes 140 are arranged on the side wall of the pointed cone 130, and the plurality of through holes 140 are distributed in a circumferential array around the axis of the pointed cone 130; the structural design can facilitate the arrangement of the through hole, so that the tube body is smoothly communicated with the inside of the reaction tube.

Further, the through hole 140 extends along the inclined plane of the pointed cone 130, and the bottom end of the through hole 140 is close to the bottom of the inclined plane of the pointed cone 130; the structure design can make the medicament in the sampling tube and the reagent tube smoothly flow into the reaction tube.

Further, the sampling tube 200 includes a first tube 220, the first film 210 is disposed at the inner side of the bottom end of the first tube 220, the cover 230 is mounted at the inner side of the top end of the first tube 220, the first insertion grooves 240 radially disposed are disposed at two sides of the top end opening of the first tube 220, and the top surface of the cover 230 after mounting is flush with the bottom of the first insertion groove 240; the sampling tube is used for placing detection solution, the cover body is arranged to be conveniently opened, and a sampling cotton swab and the solution can be conveniently and smoothly placed; the setting of first embedded groove can be used to adaptation automation equipment, provides the draw-in groove atress basis for the rotation of sampling tube.

Further, the reagent tube 300 includes a second tube 320, the top end of the second tube 320 is closed, the top surface of the second tube 320 is provided with a second insertion groove 330 arranged along the radial direction, and the second film 310 is arranged at the inner side of the bottom end of the second tube 320; the reagent tube is used for storing reaction reagents, the top end of the reagent tube is closed, the bottom end of the reagent tube is open, so that the reagents can be conveniently placed, and the reagent tube is plugged by a second film after the reagents are placed; the second embedded groove can be used for adapting to automatic equipment and providing a clamping groove stress foundation for rotation of the reagent tube; the reagent tube and the internal reagent may be prepared in advance directly in a factory, and may be used directly when the detection is required.

Further, the inner wall of the connection pipe 120 is provided with an internal thread, the outer walls of the bottoms of the first pipe 220 and the second pipe 320 are provided with an external thread, and the sampling pipe 200 and the reagent pipe 300 are installed at the corresponding connection pipe 120 in a threaded connection manner; can make first body and second body all carry out threaded connection cooperation with the takeover, convenient firm connection also can guarantee sealed condition.

Further, the inlet 110 is a bucket-shaped structure, and the diameter of the bottom opening of the inlet 110 is smaller than that of the top opening.

Further, the reaction tube 100 is a funnel-shaped structure, and the bottom end is plugged, so that the confluence and mixing of the sample solution and the detection reagent are facilitated.

While the utility model has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the utility model. The present invention is not to be limited by the specific embodiments disclosed herein, and other embodiments that fall within the scope of the claims of the present application are intended to be within the scope of the present invention.

Claims

1. A molecular detection consumable component, its characterized in that:

comprises a reaction tube, a sampling tube and a reagent tube;

the top of the reaction tube is closed, the top surface is provided with a plurality of input ports, the top surface is fixedly provided with a plurality of connecting tubes corresponding to the input ports, the top of each input port is fixedly provided with a pointed cone, and each pointed cone is provided with a through hole communicated with the inside of the reaction tube;

the bottom of the sampling tube is open and is blocked by the first film, the sampling tube is connected and matched with one of the connecting tubes, the sampling tube is pushed downwards, and the first film can be punctured by the pointed cone;

the bottom of the reagent tube is opened and is blocked by the second thin film, the rest connecting tubes are connected with the reagent tube, the reagent tube is pushed downwards, and the second thin film can be punctured by the pointed cone.

2. The molecular assay consumable assembly of claim 1, wherein:

the pointed cone body is of a thin-wall structure, the through holes are formed in the side wall of the pointed cone body, and the through holes are distributed in a circumferential array around the axis of the pointed cone body.

3. The molecular assay consumable assembly of claim 1, wherein:

the through hole extends along the inclined plane of the pointed cone, and the bottom end of the through hole is close to the bottom of the inclined plane of the pointed cone.

4. The molecular assay consumable assembly of claim 1, wherein:

the sampling tube comprises a first tube body, a first film is arranged on the inner side of the bottom end of the first tube body, a cover body is arranged on the inner side of the top end of the first tube body, first embedded grooves are formed in the two sides of an opening in the top end of the first tube body along the radial direction, and the top surface of the cover body after installation is flush with the bottom of the first embedded grooves.

5. The molecular assay consumable assembly of claim 4, wherein:

the reagent pipe comprises a second pipe body, the top end of the second pipe body is sealed, a second embedded groove which is arranged along the radial direction is formed in the top surface of the second pipe body, and the second film is arranged on the inner side of the bottom end of the second pipe body.

6. The molecular assay consumable assembly of claim 5, wherein:

the inner wall of the connecting pipe is provided with an internal thread, the outer walls of the bottoms of the first pipe body and the second pipe body are provided with external threads, and the sampling pipe and the reagent pipe are connected in a threaded manner and are arranged at the corresponding connecting pipe.

7. The molecular assay consumable assembly of claim 1, wherein:

the throwing-in opening is of a bucket-shaped structure, and the diameter of the opening at the bottom end of the throwing-in opening is smaller than that of the opening at the top end.

8. The molecular assay consumable assembly of claim 1, wherein:

the reaction tube is of a bucket-shaped structure, and the bottom end of the reaction tube is blocked.