CN215339904U - Centrifugal micro-fluidic chip divides liquid structure and centrifugal micro-fluidic chip - Google Patents
Centrifugal micro-fluidic chip divides liquid structure and centrifugal micro-fluidic chip Download PDFInfo
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- CN215339904U CN215339904U CN202023109886.3U CN202023109886U CN215339904U CN 215339904 U CN215339904 U CN 215339904U CN 202023109886 U CN202023109886 U CN 202023109886U CN 215339904 U CN215339904 U CN 215339904U
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- 239000007788 liquid Substances 0.000 title claims abstract description 126
- 238000000926 separation method Methods 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 47
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 230000004048 modification Effects 0.000 claims abstract description 6
- 238000012986 modification Methods 0.000 claims abstract description 6
- 230000036039 immunity Effects 0.000 claims description 10
- 239000011148 porous material Substances 0.000 claims description 10
- 238000011002 quantification Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 6
- 239000002699 waste material Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- 229920006267 polyester film Polymers 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
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Abstract
The utility model relates to a centrifugal micro-fluidic chip liquid separation structure and a centrifugal micro-fluidic chip. In a first centrifugal micro-fluidic chip divides liquid structure, divide the basin to connect first collecting vat through first siphon runner, divide the basin to connect the second collecting vat through second siphon runner, set up the gas pocket on the second collecting vat, first siphon runner is filled up to the priority when liquid gets into divides the basin. In the second centrifugal microfluidic chip liquid separation structure, a liquid separation groove is connected with a first collecting groove through a first siphon flow channel and is connected with a second collecting groove through a second siphon flow channel; the second collecting tank is connected with the first closed air hole tank through a flow passage; the first siphon runner and the second siphon runner are both subjected to hydrophilic modification. The utility model can realize the liquid separation of various liquid samples or reagents on the centrifugal microfluidic chip, and can be widely applied to the liquid separation of target biomolecule extracting solution and waste liquid in-vitro diagnosis and the liquid separation of sample waste liquid and detection liquid in the immune and biochemical detection processes.
Description
Technical Field
The utility model relates to the field of medical instruments, in particular to a liquid separating structure of a centrifugal micro-fluidic chip and the centrifugal micro-fluidic chip comprising the liquid separating structure.
Background
At present, centrifugal microfluidic chips are often applied to the field of POCT, and detection results can be conveniently and quickly output without the operation of professional technicians. The device is characterized in that microfluidic structures such as a liquid storage tank, a detection tank, a valve and the like are integrated on a disc-shaped chip, and the flow of microfluid is driven by centrifugal force, so that the detection and analysis of a sample are realized. The centrifugal microfluidic chip can complete the operations of pretreatment, uniform mixing, accurate volume quantification, detection and the like of a sample. In recent years, centrifugal microfluidic chips have been rapidly developed with the advantages of integration, multi-parallel detection, high throughput, low cost, automation and the like, and have been widely applied to the fields of biochemical detection, immunoassay, nucleic acid detection, biomolecule enrichment, food safety and the like.
At present, centrifugal microfluidic chip liquid separation structures reported in documents mainly use two schemes, 1. the chip liquid separation structure uses a Y-shaped flow channel and comprises a main flow channel and two branch flow channels, when a chip is centrifuged, inertial force is generated in the acceleration process of the chip, the direction of the inertial force is opposite to the rotation direction (clockwise and anticlockwise) of the chip, theoretically, the inertial force is only enough, and when the inertial force lasts for a certain time, the purpose of liquid separation can be achieved by changing the rotation direction of the chip; 2. the chip liquid separation structure is also suitable for Y-shaped runners, a drain valve or an active valve is arranged on each branch runner, and the purpose of liquid separation is achieved by controlling the opening and closing of the valves. However, both of these two liquid separation schemes have their own problems, and scheme 1 requires a sufficiently large inertial force, and the inertial force needs to be maintained in an acceleration state for a certain period of time, so that the chip needs to be equipped with a super high speed centrifuge, and when the amount of reagent to be separated is large, the separation cannot be completed when the super high speed centrifuge reaches the maximum rotation speed. Scheme 2 needs to set up the valve on the branch, but the trap has the threshold low, unstable problem, leads to the chip to divide the liquid repeatability not good, and the active valve needs additionally to increase the accessory on chip and the instrument that the chip used, makes chip manufacturing process more complicated, increases the use cost of chip, leads to being difficult to large-scale application.
SUMMERY OF THE UTILITY MODEL
The utility model provides a centrifugal micro-fluidic chip liquid separating structure and a centrifugal micro-fluidic chip comprising the same, which can be applied to separation of target biomolecule extracting solution and waste liquid in-vitro diagnosis and detection of various samples and reagents in different grooves in sequence in the process of immunity or molecule detection.
The utility model provides a liquid separating structure of a centrifugal micro-fluidic chip, which comprises a liquid separating groove, a first siphon flow channel, a second siphon flow channel, a first collecting groove, a second collecting groove and air holes, wherein the liquid separating groove is formed in the bottom of the liquid separating groove; the liquid separating tank is connected with the first collecting tank through a first siphon flow channel and is connected with the second collecting tank through a second siphon flow channel; the second collecting tank is provided with air holes; the first siphon runner and the second siphon runner are subjected to hydrophilic modification, and the hydrophilicity and the length of the first siphon runner and the second siphon runner are set so that the first siphon runner is filled with liquid preferentially when the liquid enters the liquid dividing tank.
Furthermore, the hydrophilicity of the first siphon flow channel is superior to that of the second siphon flow channel, and the length of the second siphon flow channel is longer than that of the first siphon flow channel. In short, the capillary force of the reagent in the first siphon flow channel is larger than that in the second siphon flow channel, so that the first siphon flow channel is filled with the liquid when the liquid enters the liquid dividing groove.
Furthermore, a quantitative pool and an overflow pool are contained in the first collecting tank, wherein the volume of the quantitative pool is smaller than the sample adding amount, so that the quantitative pool in the first collecting tank is filled with the reagent preferentially when the reagent enters the first collecting tank, and redundant reagent enters the overflow pool.
Furthermore, related reagents for immunity or molecular detection can be pre-embedded in the liquid separating groove, or related filter membranes or screens for target biomolecule enrichment can be pre-embedded; related reagents for immune or molecular detection can be pre-buried in the first collecting tank and the second collecting tank.
On the basis of the first liquid separation structure, the utility model also provides a second centrifugal micro-fluidic chip liquid separation structure which comprises a liquid separation groove, a first siphon flow channel, a second siphon flow channel, a first collecting groove, a second collecting groove and a first closed pore groove; the liquid separating tank is connected with the first collecting tank through a first siphon flow channel and is connected with the second collecting tank through a second siphon flow channel; the second collecting tank is connected with the first closed air hole tank through a flow passage; the first siphon runner and the second siphon runner are both subjected to hydrophilic modification.
Furthermore, a quantitative pool and an overflow pool are contained in the first collecting tank, wherein the volume of the quantitative pool is smaller than the sample adding amount, so that the quantitative pool is filled preferentially when the reagent enters the first collecting tank, and redundant samples enter the overflow pool.
Further, an Nth siphon flow channel, an Nth collecting tank and an N-1 th closed pore tank are correspondingly added on the basis of the second centrifugal micro-fluidic chip liquid separation structure, wherein N is an integer more than or equal to three; one end of the Nth siphon flow channel is connected with the liquid dividing groove, the other end of the Nth siphon flow channel is connected with the Nth collecting groove, and the Nth collecting groove is connected with the N-1 th sealed air hole groove. For example, a third siphon flow channel, a third collecting tank and a second closed pore tank can be added, so that the liquid separation of three reagents can be expanded, and by analogy, the siphon flow channel, the collecting tank and the closed pore tank are continuously added, so that the liquid separation of more than three reagents can be expanded.
Furthermore, related reagents for immunity or molecular detection can be pre-embedded in the liquid separating tank, or related filter membranes or screens for target biomolecule enrichment can be pre-embedded in the liquid separating tank, and related reagents for immunity or molecular detection can be pre-embedded in the first collecting tank and the second collecting tank.
The utility model also provides a centrifugal microfluidic chip which comprises the centrifugal microfluidic chip liquid-separating structure.
The centrifugal micro-fluidic chip liquid separation structure has the following advantages:
1. two or more reagents can enter different collecting tanks in sequence through the liquid separating structure on the centrifugal micro-fluidic chip
2. Different liquids cannot be mutually cross-polluted in the liquid separation process;
3. the chip does not need to be added with a valve, so that the whole structure is simple;
4. the liquid separating structure is formed on centrifugal chips with different functional requirements as a functional module, and can be applied to separation of target biomolecule extracting solution and waste liquid in-vitro diagnosis and detection of various samples and reagents in different grooves in sequence in the process of immunity or molecular detection.
Drawings
FIG. 1 is a schematic diagram of a first chip liquid separation structure.
FIG. 2 is a schematic diagram of a second chip liquid separation structure.
FIG. 3 is a schematic diagram of a liquid separation structure of a second chip.
FIG. 4 is a schematic structural view of a third siphon flow channel added on the basis of the second chip liquid separation structure.
FIG. 5 is a schematic view showing the addition of a sample to a sample tank.
FIG. 6 is a schematic diagram of the first centrifugation of the chip.
FIG. 7 is a schematic diagram of the second centrifugation of the chip.
FIG. 8 is a schematic diagram showing that a reagent is added into the liquid storage tank, and a needle is used to puncture the lower polyester film of the chip closed air hole tank to form air holes.
FIG. 9 shows a third centrifugation of the chip.
FIG. 10 shows a fourth centrifugation of the chip.
Detailed Description
The utility model is described in further detail below with reference to specific embodiments and the attached drawings.
Fig. 1 illustrates a first centrifugal microfluidic chip liquid-separating structure of the present invention, in which the chip includes a tightly bonded upper chip layer and a lower chip layer. The lower layer of the chip can be made of materials such as polyester film. The chip upper strata includes at least one detecting element, sets up a branch liquid structure in every detecting element, and this divides liquid structure to include: the device comprises a liquid dividing groove 11, a first siphon flow passage 12, a second siphon flow passage 13, a first collecting groove 14, a second collecting groove 15 and an air hole 16. The liquid separating tank 11 is connected with a first collecting tank 14 through a first siphon flow passage 12, and the liquid separating tank 11 is connected with a second collecting tank 15 through a second siphon flow passage 13. An air hole 16 is provided on the second collecting tank 15. The liquid separating tank 11 can be pre-embedded with relevant reagents for immunization or molecular detection or pre-embedded with relevant filter membranes or screens for target biomolecule enrichment. Relevant reagents for immune or molecular detection can be pre-buried in the collecting tanks (the first collecting tank 14 and the second collecting tank 15). The first siphon flow channel 12 and the second siphon flow channel 13 are both modified by hydrophilicity, and the hydrophilicity and the length of the first siphon flow channel 12 and the second siphon flow channel 13 are set so that the first siphon flow channel 12 is filled with liquid preferentially when the liquid enters the liquid dividing tank 11. In this embodiment, the hydrophilicity of the first siphon flow channel 12 is better than that of the second siphon flow channel 13, the length of the second siphon flow channel 13 is longer than that of the first siphon flow channel 12, in short, the capillary force of the reagent in the first siphon flow channel 12 is greater than that in the second siphon flow channel 13, so that the first siphon flow channel 12 is filled with the liquid when the liquid enters the liquid separating tank 11.
In this embodiment, the first collecting tank 13 contains a quantitative pool and an overflow pool, wherein the volume of the quantitative pool is smaller than the sample adding amount, so that the quantitative pool in the first collecting tank 13 is preferentially filled when the reagent enters the first collecting tank 13, and the redundant reagent enters the overflow pool.
The liquid separating tank 11 can be connected to a sample tank, a liquid storage tank and the like through a flow channel, is used for adding a sample or a reagent, and enters the liquid separating tank through the flow channel.
The step of adopting above-mentioned first centrifugal micro-fluidic chip to divide liquid structure to realize dividing liquid is as follows:
1) the chip is centrifuged for the first time, the reagent A enters the liquid separating tank 11, and after the centrifugation is stopped, the first siphon flow channel 12 is filled with the liquid A preferentially when the liquid A enters the liquid separating tank 11 due to the hydrophilicity and the length of the first siphon flow channel 12 and the second siphon flow channel 13;
2) the chip is centrifuged for the second time, the reagent A enters the first collecting tank 14 along the first siphon flow channel 12 and fills the quantitative pool in the tank, and the redundant sample enters the overflow pool;
3) centrifuging the chip for the third time, wherein a reagent B enters the liquid separating tank 11, because the waste liquid quantitative tank connected with the first siphon flow channel 12 is filled with liquid, the air cannot be discharged from the middle section of the first siphon flow channel 12, the first siphon flow channel 12 is changed into a normally closed valve, and the second collecting tank 15 connected with the second siphon flow channel 13 is filled with the reagent because the second siphon flow channel 13 normally works due to the air hole 16;
4) the chip is centrifuged a fourth time and reagent B is passed along second siphon flow path 13 to second collection tank 15.
Fig. 2 illustrates a second centrifugal microfluidic chip liquid separation structure of the present invention, wherein the chip comprises a bonded upper chip layer and a bonded lower chip layer. The lower layer of the chip can be made of materials such as polyester film. The chip upper strata includes at least one detecting element, sets up a branch liquid structure in every detecting element, and this divides liquid structure to include:
the device comprises a liquid dividing groove 21, a first siphon flow passage 22, a second siphon flow passage 23, a first collecting groove 24, a second collecting groove 25 and a first sealed air hole groove 26. The liquid separating tank 21 is connected to a first collecting tank 24 through a first siphon flow passage 22, and the liquid separating tank 21 is connected to a second collecting tank 25 through a second siphon flow passage 23. The second catch tank 25 is connected to the first closed-off vent tank 26 by a flow path. The liquid separating tank 21 can be pre-embedded with relevant reagents for immunization or molecular detection or pre-embedded with relevant filter membranes or screens for target biomolecule enrichment. Relevant reagents for immune or molecular detection can be pre-embedded in the collecting tanks (the first collecting tank 24 and the second collecting tank 25). Both the first siphon flow path 22 and the second siphon flow path 23 are hydrophilic-modified.
Fig. 3 is a schematic diagram of a second centrifugal microfluidic chip liquid separation structure for realizing liquid separation, a second collecting tank 25 connected to a second siphon flow channel 23 has no air hole, the second siphon flow channel 23 cannot be filled with liquid after the liquid enters a liquid separation tank 21, and only the first siphon flow channel 22 can be filled with liquid, 221 in fig. 3 is an air column, and the second siphon flow channel 23 functions as a normally-closed valve; the first closed pore groove 26 can be penetrated by a needle outside the chip, and when the first closed pore groove 26 is penetrated to become the air hole 261, the second siphon flow passage 23 can normally operate.
In this embodiment, the first collecting tank 24 contains a quantitative pool 241 and an overflow pool 242, wherein the volume of the quantitative pool is smaller than the sample volume, so that the quantitative pool 241 is preferentially filled when the reagent enters the first collecting tank 24, and the excess sample enters the overflow pool 242.
Fig. 4 is a schematic structural view of adding a third siphon flow channel on the basis of the second chip liquid separation structure. As shown in the figure, on the basis of the second centrifugal microfluidic chip liquid separation structure, a third siphon flow channel 27, a third collection tank 28 and a second closed pore tank 29 are correspondingly added, so that liquid separation of three reagents can be expanded, and by analogy, a siphon flow channel, a collection tank and a closed pore tank are continuously added, so that liquid separation of more than three reagents can be expanded.
The specific liquid separation steps for realizing liquid separation by adopting the liquid separation structure of the second centrifugal microfluidic chip are as follows:
1) the chip is centrifuged for the first time, the reagent A which is added into the sample tank 30 (as shown in FIG. 5) in advance enters the liquid separating tank 21, as shown in FIG. 6, after the centrifugation is stopped, because the second collecting tank 25 connected with the second siphon flow channel 23 has no air hole, the second siphon flow channel 23 is a normally closed valve at this time, and the sample can only fill the first siphon flow channel 22;
2) a second centrifugation is carried out, reagent a enters the first collection tank 23 along the first siphon flow path 22 and fills the dosing reservoir in the first collection tank 23, and the excess sample enters the overflow reservoir, as shown in fig. 7;
3) piercing the first closed vent groove 26 with a needle outside the chip to form a vent 261;
4) the chip is centrifuged for the third time, the reagent B which is added into the liquid storage tank 31 (shown in fig. 8) in advance enters the liquid dividing tank 21, as shown in fig. 9, because the waste liquid quantitative tank connected with the first siphon flow channel 22 is filled with liquid, the air can not be discharged from the middle section of the first siphon flow channel 22, the first siphon flow channel 22 becomes a normally closed valve, and one end of the second collecting tank 25 connected with the second siphon flow channel 23 forms an air hole after being punctured by the first airtight air hole tank 26, the second siphon flow channel 23 works normally, and the reagent fills the second siphon flow channel 23;
5) the chip is centrifuged a fourth time and the reagent flows along the second siphon flow path 23 to the second collection tank 25, as shown in fig. 10.
The particular embodiments of the present invention disclosed above are illustrative only and are not intended to be limiting, since various alternatives, modifications, and variations will be apparent to those skilled in the art without departing from the spirit and scope of the utility model. The utility model should not be limited to the disclosure of the embodiments in the present specification, but the scope of the utility model is defined by the appended claims.
Claims (9)
1. A centrifugal micro-fluidic chip liquid separation structure is characterized by comprising a liquid separation groove, a first siphon flow channel, a second siphon flow channel, a first collecting groove, a second collecting groove and air holes; the liquid separating tank is connected with the first collecting tank through a first siphon flow channel and is connected with the second collecting tank through a second siphon flow channel; the second collecting tank is provided with air holes; the first siphon runner and the second siphon runner are subjected to hydrophilic modification, and the hydrophilicity and the length of the first siphon runner and the second siphon runner are set so that the first siphon runner is filled with liquid preferentially when the liquid enters the liquid dividing tank.
2. The centrifugal microfluidic chip liquid separation structure of claim 1, wherein the first siphon flow channel has a hydrophilicity better than that of the second siphon flow channel, and the second siphon flow channel has a length longer than that of the first siphon flow channel.
3. The structure of claim 1, wherein the first collection well contains a quantification pool and an overflow pool, wherein the quantification pool has a volume smaller than the sample volume, such that the quantification pool in the first collection well is preferentially filled when the reagent enters the first collection well, and the excess reagent enters the overflow pool.
4. The liquid separation structure of the centrifugal microfluidic chip according to claim 1, wherein reagents related to immunity or molecular detection are pre-embedded in the liquid separation tank, or reagents related to immunity or molecular detection are pre-embedded in a filter membrane or a screen used for enriching target biomolecules, and the reagents related to immunity or molecular detection are pre-embedded in the first collection tank and the second collection tank.
5. A centrifugal micro-fluidic chip liquid separation structure is characterized by comprising a liquid separation groove, a first siphon flow channel, a second siphon flow channel, a first collecting groove, a second collecting groove and a first closed pore groove; the liquid separating tank is connected with the first collecting tank through a first siphon flow channel and is connected with the second collecting tank through a second siphon flow channel; the second collecting tank is connected with the first closed air hole tank through a flow passage; the first siphon runner and the second siphon runner are both subjected to hydrophilic modification.
6. The structure of claim 5, wherein the first collection well comprises a quantification chamber and an overflow chamber, wherein the quantification chamber has a volume smaller than the sample volume, such that the quantification chamber is preferentially filled with reagents when the reagents enter the first collection well, and excess samples enter the overflow chamber.
7. The centrifugal microfluidic chip liquid separation structure according to claim 5, further comprising an Nth siphon flow channel, an Nth collection tank, and an N-1 th closed pore tank, wherein N is an integer greater than or equal to three; one end of the Nth siphon flow channel is connected with the liquid dividing groove, the other end of the Nth siphon flow channel is connected with the Nth collecting groove, and the Nth collecting groove is connected with the N-1 th sealed air hole groove.
8. The liquid separation structure of the centrifugal microfluidic chip according to claim 5, wherein reagents related to immunity or molecular detection are pre-embedded in the liquid separation tank, or reagents related to immunity or molecular detection are pre-embedded in the first collection tank and the second collection tank, or a filter membrane or a screen related to target biomolecule enrichment is pre-embedded in the liquid separation tank.
9. A centrifugal microfluidic chip is characterized by comprising the centrifugal microfluidic chip liquid separating structure disclosed by any one of claims 1-8.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114660306A (en) * | 2020-12-22 | 2022-06-24 | 苏州含光微纳科技有限公司 | Centrifugal microfluidic chip liquid separation structure and liquid separation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114660306A (en) * | 2020-12-22 | 2022-06-24 | 苏州含光微纳科技有限公司 | Centrifugal microfluidic chip liquid separation structure and liquid separation method |
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