CN215313154U

CN215313154U - Cell experiment extraction element

Info

Publication number: CN215313154U
Application number: CN202120769860.7U
Authority: CN
Inventors: 衡建福
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2021-12-28
Anticipated expiration: 2031-04-15

Abstract

The utility model discloses a cell experiment extraction device which comprises a medium containing device, a top support type bottom blanking device, a centrifugal rotating device, an overhead rack and a driving device, wherein the medium containing device is arranged on the centrifugal rotating device, the top support type bottom blanking device is arranged on the medium containing device, the centrifugal rotating device is arranged on the overhead rack, and the driving device is arranged on the overhead rack. The utility model belongs to the technical field of cell separation and extraction, and particularly relates to a cell experiment extraction device, in particular to a cell experiment extraction device; according to the utility model, by discharging from the bottom of the centrifugal cup, empty space and time for transferring a large amount of media are saved, the extraction efficiency is improved, the process flow is simplified, and the possibility of cross contamination is reduced.

Description

Cell experiment extraction element

Technical Field

The utility model belongs to the technical field of cell separation and extraction, and particularly relates to a cell experiment extraction device.

Background

The differential centrifugation method is a common cell experiment extraction mode, different substances in a medium can be separated by using different centrifugation rotating speeds, the substances are extracted one by one in a multi-centrifugation mode, but the traditional operation steps are that after the centrifugation is finished, the precipitate is on the bottom layer, the supernatant needs to be led out, the precipitate at the bottom is reserved, then PBS buffer solution with the same volume is added into the supernatant, the second centrifugation is carried out at a higher rotating speed, and the medium is easy to generate cross contamination due to the step of pouring out the supernatant.

SUMMERY OF THE UTILITY MODEL

Aiming at the situation and overcoming the defects of the prior art, the utility model provides the cell experiment extraction device, through the mode of discharging from the bottom of the centrifugal cup, the vacancy and the time for transferring a large amount of media are saved, the extraction efficiency is improved, the process flow is simplified, and the probability of cross contamination is reduced.

The technical scheme adopted by the utility model is as follows: the utility model relates to a cell experiment extraction device, which comprises a medium containing device, a top support type bottom blanking device, a centrifugal rotating device, an overhead type frame and a driving device, wherein the medium containing device is arranged on the centrifugal rotating device; the medium containing device comprises a transparent centrifugal cup body and a centrifugal cup upper cover, the transparent centrifugal cup body is arranged on the centrifugal rotating device, a centrifugal cup bottom hole is formed in the transparent centrifugal cup body, a centrifugal cup annular mounting platform is arranged in the middle of the transparent centrifugal cup body, an upper cover bayonet is arranged on the centrifugal cup upper cover, and the centrifugal cup upper cover is clamped on the upper edge of the transparent centrifugal cup body through the upper cover bayonet.

Further, top supporting type bottom blanking device includes unloading sleeve, sealed dish and sealed extension spring, unloading sleeve block is located in the hole of centrifugation cup bottom, be equipped with sealed dish mesopore slide-spindle on the unloading sleeve, be equipped with sealed dish mesopore slide-spindle on the sealed dish, sealed dish slides through sealed dish mesopore slide-spindle and locates in the sleeve bottom hole, be equipped with sleeve top circular slot on the unloading sleeve, during sleeve top circular slot is located to sealed dish block, sealed dish annular equipartition is equipped with sealed dish slide-spindle side opening on sealed dish mesopore slide-spindle, the one end of sealed extension spring is located on the unloading sleeve, the other end of sealed extension spring is located on the sealed dish.

Further, centrifugal rotary device includes annular bearing frame, centrifugal bearing body, centrifugal cup mount table and the fixed lid of centrifugal cup, the annular bearing erects in the formula frame that erects, centrifugal bearing body block is located on the annular bearing frame, be equipped with mount table side draw-in groove on the centrifugal cup mount table, the centrifugal cup mount table is located on the centrifugal bearing body through mount table side draw-in groove block, be equipped with mount table bottom draw-in groove on the centrifugal cup mount table, transparent centrifugal cup body is located in mount table bottom draw-in groove through centrifugal cup annular mount table block, centrifugal cup fixed lid and centrifugal cup mount table bolted connection.

Further, the overhead type frame comprises an overhead type base, an overhead platform and a control panel, a base rib plate is arranged on the overhead type base, the overhead platform is arranged on the base rib plate, a platform round hole is formed in the overhead platform, the annular bearing is erected on the overhead platform, the transparent centrifugal cup body and the platform round hole are coaxially arranged, the control panel is arranged on the overhead platform, and a speed regulation knob is arranged on the control panel.

Further, drive arrangement includes driving motor, drive pulley, driven pulley and driving belt, driving motor locates on the built on stilts platform, the driving pulley block is located on driving motor, driving motor and control panel electric connection, the driven pulley block is located on the draw-in groove of mount table bottom, the driving belt block is located on the driving pulley, the driving belt block is located on the driven pulley.

The utility model with the structure has the following beneficial effects: this scheme cell experiment extraction element, through the mode of arranging the material from the bottom of centrifugal cup, left out the empty deficiency and the time that a large amount of media shifted, improved extraction efficiency, simplified process flow, reduced cross contamination's possibility, use outside sleeve pipe as opening device through hidden valve, leakproofness when can perfectly guarantee the unloading, the platform of design is maked somebody a mere figurehead in the cooperation, the cell experiment extraction element on the existing market step is loaded down with trivial details, inefficiency, easy cross contamination's problem has effectively been solved.

Drawings

FIG. 1 is a perspective view of a cell assay extraction device of the present invention;

FIG. 2 is an exploded view of a cell assay extraction device according to the present invention;

FIG. 3 is a front view of a cell assay extraction device of the present invention;

FIG. 4 is a top view of a cell assay extraction device of the present invention;

FIG. 5 is a cross-sectional view taken along section line A-A of FIG. 4;

fig. 6 is a partially enlarged view of a portion i in fig. 5.

Wherein, 1, a medium holding device, 2, a top support type bottom blanking device, 3, a centrifugal rotating device, 4, an overhead frame, 5, a driving device, 6, a transparent centrifugal cup body, 7, a centrifugal cup upper cover, 8, a centrifugal cup bottom hole, 9, a centrifugal cup annular mounting platform, 10, an upper cover bayonet, 11, a blanking sleeve, 12, a sealing disc, 13, a sealing tension spring, 14, a sleeve bottom hole, 15, a sleeve top circular groove, 16, a sealing disc middle hole sliding shaft, 17, a sealing disc sliding shaft side hole, 18, an annular bearing frame, 19, a centrifugal bearing body, 20, a centrifugal cup mounting platform, 21, a centrifugal cup fixing cover, 22, a mounting platform side clamping groove, 23, a mounting platform bottom clamping groove, 24, an overhead base, 25, an overhead platform, 26, a control panel, 27, a base rib plate, 28, a platform circular hole, 29, a speed regulation knob, 30, a driving motor, 31, and a driving belt pulley, 32. driven pulley, 33, drive belt.

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1-6, the cell experiment extraction device of the present invention comprises a medium holding device 1, a top-supported bottom discharging device 2, a centrifugal rotating device 3, an overhead frame 4 and a driving device 5, wherein the medium holding device 1 is arranged on the centrifugal rotating device 3, the top-supported bottom discharging device 2 is arranged on the medium holding device 1, the centrifugal rotating device 3 is arranged on the overhead frame 4, and the driving device 5 is arranged on the overhead frame 4; the medium containing device 1 comprises a transparent centrifugal cup body 6 and a centrifugal cup upper cover 7, the transparent centrifugal cup body 6 is arranged on the centrifugal rotating device 3, a centrifugal cup bottom hole 8 is formed in the transparent centrifugal cup body 6, a centrifugal cup annular mounting table 9 is arranged in the middle of the transparent centrifugal cup body 6, an upper cover bayonet 10 is formed in the centrifugal cup upper cover 7, and the centrifugal cup upper cover 7 is clamped on the upper edge of the transparent centrifugal cup body 6 through the upper cover bayonet 10.

Top supported bottom unloader 2 includes unloading sleeve 11, sealed dish 12 and sealed extension spring 13, the 11 blocks of unloading sleeve are located in centrifugal cup bottom hole 8, be equipped with sealed dish 12 mesopore slide-shaft 16 on the unloading sleeve 11, be equipped with sealed dish 12 mesopore slide-shaft 16 on the sealed dish 12, sealed dish 12 slides through sealed dish 12 mesopore slide-shaft 16 blocks and locates in sleeve bottom hole 14, be equipped with sleeve top circular slot 15 on the unloading sleeve 11, sealed dish 12 block is located in sleeve top circular slot 15, sealed dish 12 annular has all laid sealed dish 12 slide-shaft side opening 17 on sealed dish 12 mesopore slide-shaft 16, the one end of sealed extension spring 13 is located on the unloading sleeve 11, the other end of sealed extension spring 13 is located on sealed dish 12.

Centrifugal rotary device 3 includes annular bearing frame 18, centrifugal bearing body 19, centrifugal cup mount table 20 and the fixed lid 21 of centrifugal cup, annular bearing frame 18 is located on the overhead frame 4, centrifugal bearing body 19 block is located on annular bearing frame 18, be equipped with mount table side draw-in groove 22 on the centrifugal cup mount table 20, centrifugal cup mount table 20 is located on centrifugal bearing body 19 through mount table side draw-in groove 22 block, be equipped with mount table bottom draw-in groove 23 on the centrifugal cup mount table 20, transparent centrifugal cup body 6 is located in mount table bottom draw-in groove 23 through centrifugal cup annular mount table 9 block, centrifugal cup fixed lid 21 and centrifugal cup mount table 20 bolted connection.

The overhead type frame 4 comprises an overhead type base 24, an overhead platform 25 and a control panel 26, a base rib plate 27 is arranged on the overhead type base 24, the overhead platform 25 is arranged on the base rib plate 27, a platform round hole 28 is arranged on the overhead platform 25, the annular bearing frame 18 is arranged on the overhead platform 25, the transparent centrifugal cup body 6 and the platform round hole 28 are coaxially arranged, the control panel 26 is arranged on the overhead platform 25, and a speed regulation knob 29 is arranged on the control panel 26.

Drive arrangement 5 includes driving motor 30, drive pulley 31, driven pulley 32 and driving belt 33, driving motor 30 locates on the built on stilts platform 25, driving pulley 31 block is located on driving motor 30, driving motor 30 and control panel 26 electricity are connected, driven pulley 32 block is located on mount table bottom draw-in groove 23, driving belt 33 block is located on driving pulley 31, driving belt 33 block is located on driven pulley 32.

When the centrifugal cup is used, a user firstly pours a mixed medium into the transparent centrifugal cup body 6, then the transparent centrifugal cup body 6 and the centrifugal cup upper cover 7 are installed, then the speed regulation knob 29 is rotated to start the driving motor 30, the driving belt pulley 31 drives the driven belt pulley 32 to rotate under the support of the centrifugal bearing body 19 through the transmission belt 33, so as to drive the transparent centrifugal cup body 6 to rotate at a high speed, substances with different densities in the medium can be precipitated and layered when the transparent centrifugal cup body 6 rotates, the large substances are precipitated at the bottom when the transparent centrifugal cup body is rotated at a low speed, the small and medium substances are suspended in supernatant, after the separation is completed, the medium and medium substances are inserted into the sleeve bottom hole 14 through an external conduit, the hole sliding shaft 16 in the sealing disc 12 is jacked upwards, the sealing disc 12 slides out of the sleeve top circular groove 15, and the sliding shaft side hole 17 in the sealing disc 12 at the moment, the precipitate at the bottom of the transparent centrifugal cup body 6 can enter the hole sliding shaft 16 in the sealing disc 12 along the sleeve top circular groove 15 and the sliding shaft side hole 17 in the sealing disc 12, then the mixture flows into an external storage tank along an external conduit, after the external conduit is taken out, the sealing disc 12 rebounds under the tension of the sealing tension spring 13, the circular groove 15 at the top of the sleeve is sealed again, the PBS buffer solution with the same volume is added, then the second centrifugation is carried out, the speed of the second centrifugation is higher than that of the first centrifugation, the medium substances can be precipitated, the small substances can be suspended in the supernate, the substances in the medium can be separated out through different centrifugation rotating speeds, the whole working process of the utility model is completed, and the step is repeated when the mixture is used next time.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

The present invention and its embodiments have been described above, and the description is not intended to be limiting, and the drawings are only one embodiment of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the utility model as defined by the appended claims.

Claims

1. A cell experiment extraction element which characterized in that: the automatic feeding device comprises a medium containing device, a top support type bottom discharging device, a centrifugal rotating device, an overhead rack and a driving device, wherein the medium containing device is arranged on the centrifugal rotating device; the medium containing device comprises a transparent centrifugal cup body and a centrifugal cup upper cover, the transparent centrifugal cup body is arranged on the centrifugal rotating device, a centrifugal cup bottom hole is formed in the transparent centrifugal cup body, a centrifugal cup annular mounting platform is arranged in the middle of the transparent centrifugal cup body, an upper cover bayonet is arranged on the centrifugal cup upper cover, and the centrifugal cup upper cover is clamped on the upper edge of the transparent centrifugal cup body through the upper cover bayonet.

2. The extraction device for cell experiment as claimed in claim 1, wherein: top supported bottom unloader includes unloading sleeve, sealed dish and sealed extension spring, unloading sleeve block is located in the hole of centrifugation cup bottom, be equipped with sealed dish mesopore slide-spindle on the unloading sleeve, be equipped with sealed dish mesopore slide-spindle on the sealed dish, sealed dish slides through sealed dish mesopore slide-spindle and locates in the sleeve bottom hole, be equipped with sleeve top circular slot on the unloading sleeve, sealed dish block is located in the sleeve top circular slot, sealed dish annular equipartition is equipped with sealed dish slide-spindle side opening on sealed dish mesopore slide-spindle, the one end of sealed extension spring is located on the unloading sleeve, the other end of sealed extension spring is located on the sealed dish.

3. The extraction device for cell experiment as claimed in claim 2, wherein: the centrifugal rotating device comprises an annular bearing frame, a centrifugal bearing body, a centrifugal cup mounting table and a centrifugal cup fixing cover, the annular bearing frame is arranged on the overhead type rack, the centrifugal bearing body is clamped on the annular bearing frame, a mounting table side clamping groove is formed in the centrifugal cup mounting table, the centrifugal cup mounting table is clamped on the centrifugal bearing body through the mounting table side clamping groove, a mounting table bottom clamping groove is formed in the centrifugal cup mounting table, the transparent centrifugal cup body is arranged in the mounting table bottom clamping groove through the centrifugal cup annular mounting table, and the centrifugal cup fixing cover is connected with the centrifugal cup mounting table through bolts.

4. The extraction device for cell experiment as claimed in claim 3, wherein: the improved centrifugal cup is characterized in that the overhead type rack comprises an overhead type base, an overhead platform and a control panel, base rib plates are arranged on the overhead type base, the overhead platform is arranged on the base rib plates, platform round holes are formed in the overhead platform, the annular bearing is erected on the overhead platform, the transparent centrifugal cup body and the platform round holes are coaxially arranged, the control panel is arranged on the overhead platform, and a speed regulation knob is arranged on the control panel.

5. The extraction device for cell experiment as claimed in claim 4, wherein: the driving device comprises a driving motor, a driving belt pulley, a driven belt pulley and a transmission belt, the driving motor is arranged on the overhead platform, the driving belt pulley is clamped on the driving motor, the driving motor is electrically connected with the control panel, the driven belt pulley is clamped on a clamping groove at the bottom of the mounting table, the transmission belt is clamped on the driving belt pulley, and the transmission belt is clamped on the driven belt pulley.