CN214735762U - Centrifugal nuclear extraction and amplification integrated system - Google Patents
Centrifugal nuclear extraction and amplification integrated system Download PDFInfo
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- CN214735762U CN214735762U CN202022432598.5U CN202022432598U CN214735762U CN 214735762 U CN214735762 U CN 214735762U CN 202022432598 U CN202022432598 U CN 202022432598U CN 214735762 U CN214735762 U CN 214735762U
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Abstract
The utility model discloses a centrifugal nuclear amplification integrated system, which comprises a centrifugal nuclear amplification integrated instrument and a card box for nuclear amplification integrated reaction, wherein the card box comprises a nucleic acid processing area and a nucleic acid detection area, the nucleic acid processing area is communicated with the nucleic acid detection area through a channel, and a wax valve for plugging is arranged in the channel; the centrifugal nuclear extracting and amplifying integrated instrument is provided with a card box bearing mechanism, a centrifugal motion driving device, a temperature control reaction mechanism, an optical detection mechanism and a circuit control system; the system is simple to operate, small in size and high in automation degree; the system can run in a full-automatic flow only by manually adding a sample, and the instrument is operated without liquid in the whole process; the extract does not need to be transferred manually, so that cross contamination is avoided; reducing the risk of false positives in the results.
Description
Technical Field
The utility model relates to a biomedical gene analysis instrument technical field particularly, relates to a centrifugal nuclear is carried and is amplified integration system.
Background
Nucleic acid extraction and Polymerase Chain Reaction (PCR) are common analytical tools in molecular biology. Conventionally, traditional nucleic acid extraction and PCR are performed in separate instruments, and after sample pretreatment is completed, an experimenter needs to manually perform sample loading for check extraction, and after the check extraction is completed, a PCR experimental system is manually prepared and tested on a computer, so that the whole process is finished, at least half a day is needed, the analysis and test time is too long, and time and labor are wasted.
In addition, the separate procedures of the nuclear PCR are prone to cause cross contamination and environmental pollution, which brings false positive of the detection result and affects the result analysis.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned technical problem among the correlation technique, the utility model provides an amplification integration system is carried to centrifugal nuclear can overcome the above-mentioned not enough of prior art, can shorten analysis and detection time, has reduced cross contamination's possibility, and the testing result is more accurate.
In order to achieve the technical purpose, the technical scheme of the utility model is realized as follows: a centrifugal nuclear extraction and amplification integrated system and a detection method comprise a centrifugal nuclear extraction and amplification integrated instrument and a card box for carrying out a nuclear extraction and amplification integrated reaction, wherein the card box comprises a nucleic acid processing area and a nucleic acid detection area, the nucleic acid processing area is communicated with the nucleic acid detection area through a channel, and a wax valve for plugging is arranged in the channel;
the centrifugal nuclear lifting amplification integrated instrument is provided with a card box bearing mechanism, a centrifugal motion driving device, a temperature control reaction mechanism, an optical detection mechanism and a circuit control system.
Furthermore, a liquid storage end which is formed by injection molding of a chemical inert material is arranged on the card box, a channel connected with the liquid storage end and an opening are sealed by a membrane or a glass slide which is made of the inert material, and the sealing mode is adhesive, UV (ultraviolet) glue exposure or ultrasonic welding.
Furthermore, the number of the nucleic acid detection areas is one or more, a reaction reagent is pre-embedded in the nucleic acid detection areas, and the reaction reagent is in the form of dry powder, freeze-dried balls or a liquid reaction system.
Further, the cartridge further comprises a waste liquid region and a liquid injection region; the liquid injection area and the nucleic acid treatment area as well as the nucleic acid treatment area and the waste liquid area are communicated through a channel, and a wax valve for plugging is arranged in the channel; the number of the liquid injection areas is one or more, the nuclear extraction reaction reagent is pre-embedded in the liquid injection areas, and the nuclear extraction reaction reagent is in the form of dry powder, freeze-dried balls or a liquid reaction system.
Further, the card box can adopt the magnetic bead method or the nucleic acid extraction and purification of the centrifugal column method; the magnetic bead method nucleic acid extraction and purification mode is characterized in that a magnetic bead adsorption mechanism which is aligned with the magnetic beads in the nucleic acid processing area is arranged on the centrifugal nuclear extraction and amplification integrated instrument; the centrifugal column is arranged in the middle of the nucleic acid processing area.
Furthermore, the card box is fixed above the card box supporting mechanism, at least two card boxes are supported, and the two card boxes are arranged in an axisymmetric or centrosymmetric mode;
the temperature control reaction mechanism is positioned above the card box bearing mechanism, the nucleic acid detection area of the card box is in close contact with the temperature control reaction mechanism, and the optical detection mechanism is fixed outside the centrifugal motion driving device.
Further, the wax melting mechanism is adopted to melt wax in a contact manner or in a non-contact manner; the wax melting mechanism adopts contact type wax melting, and the upper surface of the card box supporting mechanism and the position of the card box wax valve are provided with temperature control points which are correspondingly contacted; in the non-contact wax melting mode, the wax melting mechanism is fixed on the outer side of the centrifugal mechanism and can be heated by infrared irradiation.
Further, the cartridge support mechanism performs a centrifugal rotational motion at a rotational speed set by a circuit control system, and each of the nucleic acid detection regions of the cartridge is sequentially subjected to optical signal detection by an optical detection mechanism.
In another aspect of the present invention, a centrifugal nuclear amplification integrated detection method is provided, which includes a fluorescence detection method and a color development detection method;
in the fluorescence detection method, the optical detection mechanism is connected with a detection light path through an optical fiber, the optical fiber is fixedly aligned to a nucleic acid detection area for one circle, when the nucleic acid detection area rotates through the path of the optical fiber, an excitation light source of the detection light path emits light to enter the nucleic acid detection area and enter a detection device, and the detection device is converted into an electric signal and transmitted to a circuit control system;
in the color development detection method, an excitation light source and a detection device are respectively arranged on the upper side and the lower side or the inner side and the outer side of the nucleic acid detection area, light emitted by the excitation light source enters the nucleic acid detection area, and light absorbed by a color development system enters the detection device on the other side of the excitation light source.
Further, in the fluorescence detection method, the excitation light source and the detection device are respectively arranged in different directions of a circumference where the nucleic acid detection region is located, the excitation light obliquely enters the nucleic acid detection region, and emitted light enters the other detection device through a filter;
in the color development detection method, an angle is formed between the excitation light source and the detection device, light emitted by the excitation light source enters the nucleic acid detection area, and light absorbed by the color development system enters the detection device;
in the fluorescence detection method, the optical detection mechanism detects at least one optical channel.
The utility model has the advantages that: the system is simple to operate, small in size and high in automation degree; the system can run in a full-automatic flow only by manually adding a sample, and the instrument is operated without liquid in the whole process; the extract does not need to be transferred manually, so that cross contamination is avoided; reducing the risk of false positives in the results; the extracted nucleic acid template is directly subjected to amplification reaction on the same card box, so that reaction liquid in different reaction card boxes can be centrifuged into related areas at one time, and the time for liquid transfer is reduced while the automatic operation is realized; the rotation mode enables the card box to pass through the PCR optical detection mechanism in turn, and reduces the scanning time or imaging time of the optical mechanism in the traditional mode. In addition, the system is particularly suitable for non-specific PCR laboratory environments such as emergency treatment, small and medium hospitals and the like, and has no professional requirements on operators.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of a cartridge according to an embodiment of the present invention
FIG. 2 is a schematic diagram of a cartridge according to an embodiment of the present invention, which employs a centrifugal column method;
FIG. 3 is a schematic diagram of a magnetic bead method for a cartridge according to an embodiment of the present invention;
FIG. 4 is a schematic cross-sectional view of a centrifugal nuclear lifting and amplification integrated instrument according to an embodiment of the present invention;
FIG. 5 is a schematic view of a fluorescence detection structure according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of a fluorescence detection structure according to an embodiment of the present invention;
FIG. 7 is a first schematic view of a structure of a transmission detection by a color development method according to an embodiment of the present invention;
FIG. 8 is a schematic diagram of a structure of a transmission detection by a color development method according to an embodiment of the present invention;
FIG. 9 is a first schematic view of a colorimetric reflectance measurement structure according to an embodiment of the present invention;
fig. 10 is a schematic diagram of a colorimetric reflectance detection structure according to an embodiment of the present invention.
In the figure: 10. a card box; 10-1, a waste liquid area; 10-2, an injection region; 10-3, a sample area; 10-4, a lytic binding domain; 10-5, a cleaning solution area; 10-6, an eluent zone; 20. a centrifugal nuclear extraction and amplification integrated instrument; 1. a nucleic acid treatment zone; 1-1, centrifugal column; 1-2, a magnet; 1-3, a magnet movement track; 2. a nucleic acid detection region; 3. a wax valve; 4. a card box supporting mechanism; 5. a centrifugal motion drive device; 6. a temperature control reaction mechanism; 7. an optical detection mechanism; 8. a temperature control reaction mechanism; 7-1 and optical fibers.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.
As shown in fig. 1-4, a centrifugal-type integrated nuclear amplification system according to an embodiment of the present invention includes a centrifugal-type integrated nuclear amplification apparatus 20 and a cartridge 10 for performing an integrated nuclear amplification reaction, wherein the cartridge 10 includes a nucleic acid processing region 1 and a nucleic acid detection region 2, the nucleic acid processing region 1 and the nucleic acid detection region 2 are communicated with each other through a channel, and a wax valve 3 for plugging is disposed in the channel;
the centrifugal nuclear lifting amplification integrated instrument 20 is provided with a card box supporting mechanism 4, a centrifugal motion driving device 5, a temperature control reaction mechanism 6, an optical detection mechanism 7 and a circuit control system.
The utility model discloses a concrete embodiment, be equipped with the stock solution end that adopts chemical inert material injection molding to form on the card box 10, the sealed diaphragm or the slide that adopts inert material of channel and opening that the stock solution end is connected, sealed mode is viscose, UV glues exposure or ultrasonic bonding.
In a specific embodiment of the present invention, the number of the nucleic acid detecting regions 2 is one or more, the nucleic acid detecting regions 2 are pre-embedded with a reaction reagent, and the reaction reagent is in the form of dry powder, lyophilized pellet or liquid reaction system.
In one embodiment of the present invention, the cartridge 10 further comprises a waste liquid region 10-1 and a liquid injection region 10-2; the liquid injection region 10-2 and the nucleic acid treatment region 1 and the waste liquid region 10-1 are communicated through a channel, and a wax valve 3 for plugging is arranged in the channel; the number of the liquid injection areas 10-2 is one or more, the liquid injection areas 10-2 are pre-embedded with nuclear extraction reaction reagents, and the nuclear extraction reaction reagents are in the forms of dry powder, freeze-dried spheres or liquid reaction systems.
In one embodiment of the present invention, the cartridge 10 can be used for nucleic acid extraction and purification by magnetic bead method or centrifugal column method; in the magnetic bead method nucleic acid extraction and purification mode, the centrifugal nuclear extraction and amplification integrated instrument 20 is provided with a magnetic bead adsorption mechanism which is aligned with the magnetic beads in the nucleic acid processing area 1; the centrifugal column nucleic acid extraction and purification mode is characterized in that a centrifugal column is added in the middle of the nucleic acid treatment area 1.
In a specific embodiment of the present invention, the cartridge 10 is fixed above the cartridge supporting mechanism 4, the cartridge supporting mechanism 4 at least supports two of the cartridges 10, and the two cartridges 10 are arranged in an axisymmetric or centrosymmetric manner;
the temperature control reaction mechanism 6 is positioned above the card box supporting mechanism 4, the nucleic acid detection zone 2 of the card box 10 is in close contact with the temperature control reaction mechanism 6, and the optical detection mechanism 7 is fixed outside the centrifugal motion driving device 5.
In a specific embodiment of the present invention, the device further comprises a wax melting mechanism, wherein the wax melting mechanism adopts contact type wax melting or non-contact type wax melting; the wax melting mechanism adopts contact type wax melting, and the position of the upper surface of the card box supporting mechanism 4 and the wax valve of the card box 10 is provided with a temperature control point which is correspondingly contacted; in the non-contact wax melting mode, the wax melting mechanism is fixed on the outer side of the centrifugal mechanism and can be heated by infrared irradiation.
In one embodiment of the present invention, the cartridge supporting mechanism 4 performs a centrifugal rotation motion at a rotation speed set by a circuit control system, and each of the nucleic acid detecting regions 1 of the cartridge 10 sequentially performs an optical signal detection by the optical detection mechanism 7.
As shown in FIGS. 5-10, in another aspect of the present invention, a centrifugal nuclear lifting and amplification integrated detection method is provided, which comprises a fluorescence detection method and a color development detection method;
in the fluorescence detection method, the optical detection mechanism 7 is connected with a detection light path through an optical fiber 7-1, the optical fiber 7-1 is fixed and aligned with one circle of the nucleic acid detection area 1, when the nucleic acid detection area 1 rotates through the path of the optical fiber 7-1, an excitation light source of the detection light path emits light to enter the nucleic acid detection area 1 and to enter a detection device, and the detection device converts the light into an electric signal and transmits the electric signal to a circuit control system;
in the color development detection method, an excitation light source and a detection device are respectively arranged on the upper side and the lower side or the inner side and the outer side of the nucleic acid detection area 1, light emitted by the excitation light source enters the nucleic acid detection area 1, and light absorbed by a color development system enters the detection device on the other side of the excitation light source.
In a specific embodiment of the present invention, in the fluorescence detection method, the excitation light source and the detection device are respectively disposed in different directions of a circumference where the nucleic acid detection region 1 is located, the excitation light is obliquely incident on the nucleic acid detection region 1, and the emitted light is incident on the detection device disposed on the other side through a filter;
in the color development detection method, the excitation light source and the detection device are arranged at an angle, light emitted by the excitation light source enters the nucleic acid detection region 1, and light absorbed by a color development system enters the detection device;
in the fluorescence detection method, the optical detection mechanism 7 performs detection of at least one optical channel.
The utility model discloses a specific embodiment, nucleic acid detection zone 2 is equipped with 4 detection ponds, and the nucleic acid after 1 processing in nucleic acid processing zone can be through the serial or parallel a plurality of nucleic acid detection ponds of entering of intercommunication channel, and with the system reaction of embedding in the detection pond in advance, can carry out the detection of many targets of single sample.
For the convenience of understanding the above technical solutions of the present invention, the above technical solutions of the present invention are explained in detail through specific use modes below.
The cartridge 10 is a carrier for an automated nucleic acid amplification reaction, and comprises a nucleic acid processing region 1, a nucleic acid detection region 2 and a waste liquid region 10-1, wherein the regions are communicated through a channel, and a wax valve is arranged in the channel for sealing.
The liquid injection zone 10-2 comprises a sample zone 10-3, a lysis binding zone 10-4, a cleaning liquid zone 10-5 and an eluent zone 10-6;
the centrifugal nuclear extracting and amplifying integrated instrument comprises a card box supporting mechanism 4, a centrifugal motion driving device 5, a temperature control reaction mechanism 6, an optical detection mechanism 7 and a circuit control system.
The pre-buried nucleic acid detect reagent of card box 10's nucleic acid detection zone adopts the fluorescence PCR amplification detection method, and card box 10 is equipped with 4 detection zones, and the nucleic acid of drawing through integrated system promptly can distribute 4 detection zones and carry out nucleic acid amplification and detection respectively.
The temperature control reaction mechanism 6 provides the temperature environment required by nucleic acid extraction and amplification reaction for the contacted nucleic acid detection area of the card box 10, and the optical detection mechanism 7 carries out excitation and fluorescence signal acquisition on the reaction system of the nucleic acid detection area through an excitation light source.
Card box bearing mechanism 4 comprises platform motor, transmission and platform supporting component, and transmission in this embodiment is band pulley and hold-in range, and the platform motor passes through transmission and drives card box bearing mechanism 4 and carry out circular motion.
The temperature control reaction mechanism 6 provides a temperature environment of 4-100 ℃ for PCR reaction, and comprises a PCR reaction pool adapter, a temperature sensor, a Peltier and a heat radiating fin. The Peltier provides a temperature rise and fall power source, the temperature sensor feeds back the PCR reaction pool adaptor in real time, and the circuit control system controls the temperature of the Peltier through PID control according to the feedback temperature of the temperature sensor so as to control the PCR reaction pool adaptor to rise and fall at high speed; the heat sink can conduct away heat generated by the Peltier.
The optical detection mechanism 7 can detect 4 groups of optical paths, each optical path is provided with an independent optical detection module, each group of optical detection modules is provided with an excitation light source, excitation light emitted by the excitation light source irradiates the nucleic acid detection area 2 of the card box 10 through the excitation light source via an excitation light filter, a collimating lens, a dichroic mirror and a converging lens, a reaction system in the reaction tank is excited to emit fluorescence, the excited fluorescence enters the dichroic mirror through the excitation light source via the converging lens in a collimation manner, and then enters the detector via the converging lens at the emission end and the excitation light filter to be collected; the 4 optical detection channels are provided with excitation light sources with different spectrums, filter sets and detection devices to form independent light paths. 4 sets of optical paths are arranged in parallel.
Adopting a centrifugal column method for nuclear extraction:
specifically, before reaction, a sample is added into the sample area 10-3, the wax valve of the nucleic acid processing area 1 is heated to communicate with the closed channel, the sample is transferred into the nucleic acid processing area 1 through centrifugal motion, the lysis binding area 10-4 pre-packaged with a lysis binding system enters the nucleic acid processing area 1 through centrifugal motion, and the mixing reaction with the sample is realized;
after the reaction, quickly centrifuging at a certain rotating speed, allowing the reaction solution to pass through a centrifugal column 1-1 to enable nucleic acid to be in the centrifugal column 1-1, and allowing waste liquid passing through the centrifugal column to enter a waste liquid area 10-1;
opening a wax valve 3 of a cleaning solution area 10-5 by hot melting, enabling the cleaning solution area 10-5 to enter a nucleic acid processing area 1 through a channel, quickly centrifuging, and enabling the cleaned waste liquid to enter a waste solution area 10-1;
the wax valve 3 of the eluent area 10-6 is opened by hot melting, the eluent area 10-6 enters the nucleic acid processing area 1 through a channel, the nucleic acid is quickly centrifuged, the eluted nucleic acid is positioned at the bottom of the nucleic acid processing area 1 after passing through the centrifugal column 1-1, the channel communicated with the waste liquid area 10-1 is positioned at the high position of the nucleic acid processing area 1 due to less eluent, the centrifugal rotating speed is controlled, and the eluted nucleic acid cannot enter the waste liquid area 10-1.
Opening a wax valve 3 between the nucleic acid processing area 1 and the nucleic acid detection area 2, rotating in the reverse direction, so that the nucleic acid passes through the communication channel and sequentially enters the nucleic acid detection area 2, and redundant nucleic acid enters the nucleic acid waste liquid area; nucleic acid mixes with 3 amplification system that presets in nucleic acid detection zone 2 respectively, carries out PCR reaction under the temperature effect of control by temperature change reaction mechanism 6, and in the amplification reaction process, through the cooperation operation of card box supporting mechanism 4 and optical detection mechanism 7, can realize the real-time detection to the amplification product fluorescence signal in nucleic acid detection zone 2, accomplish the overall process of nucleic acid extraction and amplification integration so far.
The magnetic bead method is adopted for the nuclear extraction process:
specifically, before reaction, a sample is added into the sample area 10-3, the wax valve of the nucleic acid processing area 1 is heated to communicate with the closed channel, the sample is transferred into the nucleic acid processing area 1 through centrifugal motion, the lysis binding area 10-4 pre-packaged with a lysis binding system enters the nucleic acid processing area 1 through centrifugal motion, and the mixing reaction with the sample is realized;
the lysis binding area 10-4 which is pre-packaged with the lysis binding system enters the nucleic acid processing area 1 through centrifugal movement to realize mixing reaction with the sample. The system can be mixed in a positive and negative rotation manner in the reaction process.
After the reaction, the reaction product is quickly centrifuged at a certain rotating speed, the nucleic acid adsorbed on the surfaces of the magnetic beads is magnetically adsorbed to one side of the nucleic acid treatment area 1 by a magnet 1-2 in a magnet movement track 1-3, and the waste liquid enters a waste liquid area 10-1 through a channel;
opening a wax valve 3 of a cleaning solution area 10-5 by hot melting, enabling the cleaning solution area 10-5 to enter a nucleic acid processing area 1 through a channel, uniformly mixing the nucleic acid processing area with the cleaning solution area, quickly centrifuging, and enabling the cleaned waste liquid to enter a waste liquid area 10-1;
opening a wax valve 3 of an eluent region 10-6 by hot melting, enabling the eluent region 10-6 to enter a nucleic acid processing region 1 through a channel, after a certain time, enabling eluted nucleic acid to be at the bottom of the nucleic acid processing region 1, opening the wax valve 3, rotating in the reverse direction, enabling magnetic beads in a system to be adsorbed by a magnet 1-2, enabling the nucleic acid to sequentially pass through a channel and enter a nucleic acid detection region 2, and enabling redundant nucleic acid to enter a nucleic acid waste liquid region;
nucleic acid mixes with the amplification system that presets in the nucleic acid detection zone 2 respectively, carries out PCR reaction under the temperature effect of control by temperature change reaction mechanism 6, and in the amplification reaction process, through the cooperation operation of card box supporting mechanism 4 and optical detection mechanism 7, can realize the real-time detection to the amplification product fluorescence signal in the nucleic acid detection zone 2, accomplish the overall process of nucleic acid extraction and amplification integration so far.
In conclusion, by means of the technical scheme of the utility model, the system is simple in operation, small in size and high in automation degree; the system can run in a full-automatic flow only by manually adding a sample, and the instrument is operated without liquid in the whole process; the extract does not need to be transferred manually, so that cross contamination is avoided; reducing the risk of false positives in the results;
the extracted nucleic acid template is directly subjected to amplification reaction on the same card box, so that reaction liquid in different reaction card boxes can be centrifuged into related areas at one time, and the time for liquid transfer is reduced while the automatic operation is realized; the rotation mode enables the card box to pass through the PCR optical detection mechanism in turn, and reduces the scanning time or imaging time of the optical mechanism in the traditional mode. In addition, the system is particularly suitable for non-specific PCR laboratory environments such as emergency treatment, small and medium-sized hospitals and the like, and has no professional requirements on operators.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A centrifugal nucleic acid amplification integrated system is characterized by comprising a centrifugal nucleic acid amplification integrated instrument (20) and a cartridge (10) for carrying out a nucleic acid amplification integrated reaction, wherein the cartridge (10) comprises a nucleic acid processing area (1) and a nucleic acid detection area (2), the nucleic acid processing area (1) is communicated with the nucleic acid detection area (2) through a channel, and a wax valve (3) for plugging is arranged in the channel;
the centrifugal nuclear lifting amplification integrated instrument (20) is provided with a card box supporting mechanism (4), a centrifugal motion driving device (5), a temperature control reaction mechanism (6), an optical detection mechanism (7) and a circuit control system.
2. The centrifugal nuclear lifting and amplification integrated system according to claim 1, wherein a liquid storage end made of a chemically inert material through injection molding is arranged on the cartridge (10), a channel connected with the liquid storage end and an opening are sealed by a membrane or a glass slide made of an inert material, and the sealing mode is adhesive, UV (ultraviolet) glue exposure or ultrasonic welding.
3. The centrifugal nuclear lifting and amplification integrated system according to claim 1, wherein the number of the nucleic acid detection areas (2) is one or more, the nucleic acid detection areas (2) are pre-embedded with a reaction reagent, and the reaction reagent is in the form of dry powder, freeze-dried balls or a liquid reaction system.
4. The centrifugal nuclear lifting and amplification integrated system according to claim 1, wherein the cartridge (10) further comprises a waste liquid area (10-1) and a liquid injection area (10-2); the liquid injection region (10-2) is communicated with the nucleic acid processing region (1) and the nucleic acid processing region (1) is communicated with the waste liquid region (10-1) through a channel, and a wax valve (3) for plugging is arranged in the channel; the number of the liquid injection areas (10-2) is one or more, the liquid injection areas (10-2) are pre-embedded with nuclear extraction reaction reagents, and the nuclear extraction reaction reagents are in the forms of dry powder, freeze-dried balls or liquid reaction systems.
5. The centrifugal nucleic acid extraction and amplification integrated system according to claim 1, wherein a magnetic bead adsorption mechanism aligned with the magnetic beads of the nucleic acid processing region (1) is provided on the centrifugal nucleic acid extraction and amplification integrated instrument (20); a centrifugal column is added in the middle of the nucleic acid processing area (1).
6. A centrifugal nuclear lifting and amplification integrated system according to claim 1, wherein the cartridge (10) is fixed above the cartridge support mechanism (4), the cartridge support mechanism (4) carries at least two cartridges (10), and the two cartridges (10) are arranged in an axisymmetric or centrosymmetric manner; temperature control reaction mechanism (6) are located card box supporting mechanism (4) top, card box (10) nucleic acid detection zone (2) with temperature control reaction mechanism (6) in close contact with, optics detection mechanism (7) are fixed in the centrifugal motion drive arrangement (5) outside.
7. The integrated centrifugal nuclear lifting and amplification system of claim 1, further comprising a wax melting mechanism, wherein the wax melting mechanism adopts contact wax melting or non-contact wax melting; the wax melting mechanism adopts contact type wax melting, and the positions of the upper surface of the card box supporting mechanism (4) and the wax valve (3) of the card box (10) are provided with temperature control points which are correspondingly contacted; in the non-contact wax melting mode, the wax melting mechanism is fixed on the outer side of the centrifugal mechanism and can be heated by infrared irradiation.
8. The centrifugal nucleic acid amplification integrated system according to claim 1, wherein the cartridge support mechanism (4) performs centrifugal rotation at a rotation speed set by an electronic control system, and each nucleic acid detection region (2) of the cartridge (10) is sequentially subjected to optical signal detection by the optical detection mechanism (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202022432598.5U CN214735762U (en) | 2020-10-28 | 2020-10-28 | Centrifugal nuclear extraction and amplification integrated system |
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| CN202022432598.5U CN214735762U (en) | 2020-10-28 | 2020-10-28 | Centrifugal nuclear extraction and amplification integrated system |
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| CN214735762U true CN214735762U (en) | 2021-11-16 |
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| CN202022432598.5U Active CN214735762U (en) | 2020-10-28 | 2020-10-28 | Centrifugal nuclear extraction and amplification integrated system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226350A (en) * | 2020-10-28 | 2021-01-15 | 北京贝泰科技有限公司 | Centrifugal nuclear extraction and amplification integrated system and detection method |
| CN115433669A (en) * | 2022-08-31 | 2022-12-06 | 深圳市合川医疗科技有限公司 | A microfluidic device for pathogen detection |
| WO2023160622A1 (en) * | 2022-02-25 | 2023-08-31 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis apparatus-based control method and molecular diagnosis apparatus |
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2020
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226350A (en) * | 2020-10-28 | 2021-01-15 | 北京贝泰科技有限公司 | Centrifugal nuclear extraction and amplification integrated system and detection method |
| CN112226350B (en) * | 2020-10-28 | 2024-06-25 | 北京贝泰科技有限公司 | Centrifugal type nuclear extraction and amplification integrated system and detection method |
| WO2023160622A1 (en) * | 2022-02-25 | 2023-08-31 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis apparatus-based control method and molecular diagnosis apparatus |
| CN115433669A (en) * | 2022-08-31 | 2022-12-06 | 深圳市合川医疗科技有限公司 | A microfluidic device for pathogen detection |
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