CN214654977U - Sample reaction structure - Google Patents
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- CN214654977U CN214654977U CN202120717262.5U CN202120717262U CN214654977U CN 214654977 U CN214654977 U CN 214654977U CN 202120717262 U CN202120717262 U CN 202120717262U CN 214654977 U CN214654977 U CN 214654977U
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 25
- 239000012429 reaction media Substances 0.000 claims abstract description 11
- 238000001816 cooling Methods 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 230000001105 regulatory effect Effects 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 15
- 239000004065 semiconductor Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 19
- 239000000523 sample Substances 0.000 description 60
- 238000000034 method Methods 0.000 description 16
- 238000005516 engineering process Methods 0.000 description 7
- 230000007246 mechanism Effects 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 230000036632 reaction speed Effects 0.000 description 2
- 238000005452 bending Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
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- Bioinformatics & Cheminformatics (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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Abstract
The utility model provides a sample reaction structure has solved the technical problem that sample detection efficiency is low among the prior art. A sample reaction structure comprises a memory for storing a reaction medium and a temperature regulator for repeatedly heating and cooling a sample, wherein the memory is formed by folding a same bendable soft film, and the memory is supported after the reaction medium is stored in the memory so that the side wall of the memory is in contact with the temperature regulator. The scheme enables the storage to have larger contact area with the temperature regulator, and improves the heat exchange efficiency of the storage and the heat exchanger, thereby improving the detection efficiency of the sample.
Description
Technical Field
The present disclosure relates to the field of sample extraction technologies, and in particular, to a sample reaction structure.
Background
In the field of gene detection technology, frequent temperature increase and decrease operations are required to be performed on a sample, and the sample is generally circulated between 60 ℃ and 95 ℃ so that the sample can form good reactions with reagents, probes and the like, and the detection precision of the sample can be improved. Typically, the sample is placed in a test tube, and the test tube is subjected to repeated warming and cooling operations.
In the prior art, a test tube is used for storing a sample, and in the practical application process, the test tube has a thicker wall thickness, so that the required heating and cooling time is longer, and the test tube has the characteristic of high cost. In addition, among the prior art, because the test tube is circular, heating or cooling process to the test tube in, the area of contact of temperature regulator and test tube is little, further makes the sample rise or fall the operation of warm and need longer time, has reduced the detection efficiency of sample.
SUMMERY OF THE UTILITY MODEL
The utility model provides a sample reaction structure has solved the technical problem that sample detection efficiency is low among the prior art.
Some embodiments adopted to solve the above technical problems include:
a sample reaction structure comprises a memory for storing a reaction medium and a temperature regulator for repeatedly heating and cooling a sample, wherein the memory is formed by folding a same bendable soft film, and the memory is supported after the reaction medium is stored in the memory so that the side wall of the memory is in contact with the temperature regulator.
In the practical application process, the memory is made of a soft film, and the memory has a smaller wall thickness, so that the memory and the temperature regulator have higher heat exchange efficiency, and the detection efficiency of the sample is improved. In addition, the soft film has the bending capacity, and the sample enters the memory to enable the memory to generate certain deformation, so that the memory and the temperature regulator have larger contact area, the heat exchange efficiency of the memory and the heat exchanger is further improved, and the detection efficiency of the sample is further improved. In addition, the soft film has the advantages of low cost and easiness in processing, and the detection cost of the sample is reduced.
Preferably, the temperature controller includes a first temperature controller and a second temperature controller, the memory is located between the first temperature controller and the second temperature controller, and the memory is supported after the reaction medium is stored in the memory such that a sidewall of the memory is in contact with the first temperature controller and the second temperature controller, respectively.
In this scheme, temperature regulator heats up and cools down the operation to the memory simultaneously from the both sides of memory, has improved the efficiency of heating up and cooling down of memory greatly, and then has improved the detection efficiency of sample.
Preferably, a gap through which light passes is formed between the first temperature adjustment portion and the second temperature adjustment portion, and the memory includes a folded portion, at least a part of which is located in the gap.
In the scheme, the arrangement mode of the gap and the folding part can enable the detection unit of the related instrument to directly detect the memory, so that the structure of the reaction structure is simplified, and the use cost of the reaction structure is reduced.
Preferably, the accumulator includes a storage chamber, and a contact area of the accumulator with the first temperature adjustment portion and the second temperature adjustment portion is not smaller than a side surface area of the storage chamber having a maximum area.
In the scheme, the memory and the temperature regulator have larger contact area, and the detection efficiency of the sample is improved.
Preferably, the reservoir further comprises a seal forming a storage chamber, the seal being perpendicular to the fold.
In the scheme, the storage can be conveniently made to have a stable structure by arranging the edge sealing, the storage is easy to process, the manufacturing cost of the storage is reduced, the stability of the storage in the application process is improved, and a sample is not easy to leak.
Preferably, the sealing edge has a width not less than twice the thickness of the film from which the reservoir is made.
The width of banding has been injectd to this scheme, makes the memory have better stability, and the memory is difficult damaged.
Preferably, a temperature adjusting area is formed between the first temperature adjusting part and the second temperature adjusting part, and the sealing edge is located outside the temperature adjusting area.
This scheme makes the banding be located the temperature regulation district beyond, avoids the banding to be heated and damages, has improved the stability in the memory working process.
Preferably, the temperature regulator is a semiconductor temperature regulating sheet.
In the scheme, the semiconductor temperature adjusting sheet has good temperature adjusting capacity, the performance of the sample reaction structure is optimized, and the detection efficiency of the sample is improved.
Compared with the prior art, the sample reaction structure provided by the present disclosure has the following advantages:
1. the contact area between the memory and the temperature regulator is large, heat exchange between the memory and the temperature regulator is facilitated, and the detection efficiency of the sample is improved.
2. The storage is made of foldable soft films, the storage is easy to process, the manufacturing cost of the storage is reduced, and the use cost of the sample reactor is further reduced.
3. The storage is made of a soft film, so that the vacuum effect is easily formed in the storage, the sample is not easily polluted by air, and the detection precision of the sample is improved.
Drawings
For purposes of explanation, several embodiments of the disclosed technology are set forth in the following figures. The following drawings are incorporated herein and constitute a part of the detailed description. In some instances, well-known structures and components are shown in block diagram form in order to avoid obscuring the concepts of the disclosed subject technology.
FIG. 1 is a schematic diagram of a memory according to the present disclosure.
Fig. 2 is a schematic structural diagram of the present disclosure.
Fig. 3 is a schematic diagram of the application process of the present disclosure.
Shown in the figure:
1. memory 101, folded part, 102, banding, 2, temperature controller, 201, first temperature controller, 202, second temperature controller, 203, clearance.
Detailed Description
The specific embodiments illustrated below are intended as descriptions of various configurations of the presently disclosed subject technology and are not intended to represent the only configurations in which the presently disclosed subject technology may be practiced. Specific embodiments include specific details for the purpose of providing a thorough understanding of the presently disclosed subject matter technology. It will be apparent, however, to one skilled in the art that the disclosed subject matter technology is not limited to the specific details shown herein and may be practiced without these specific details.
Referring to fig. 1, 2 and 3, a sample reaction structure includes a memory 1 for storing a reaction medium and a temperature controller 2 for repeatedly heating and cooling a sample, wherein the memory 1 is formed by folding a same flexible film, and the memory 1 is supported after the reaction medium is stored in the memory 1 so that a sidewall of the memory 1 contacts the temperature controller 2.
The flexible film may be any flexible film in the prior art, wherein the flexible film should not chemically react with the sample and the reagent to prevent the sample from being contaminated because the storage 1 is used for storing the sample, and the sample is generally mixed in the reagent. In general, a PE film may be used as the soft film.
The temperature controller 2 is mainly used to circulate the sample between 60 degrees celsius and 95 degrees celsius, and therefore, the temperature controller 2 should have both cooling and heating capabilities. The temperature regulator 2 can adopt a semiconductor temperature regulating sheet, and the semiconductor temperature regulating sheet can be switched between refrigeration and heating by switching the polarity of current in the use process, so that the semiconductor temperature regulating sheet can meet the functional requirements of the temperature regulator 2.
In the practical application process, the flexible film is folded to form an open space, the space formed by the flexible film can be closed into a part to form a semi-closed space by utilizing the processes of heat sealing and the like, and the sample and the reagent are placed in the semi-closed space, at the moment, the flexible film can generate a certain amount of deformation due to the addition of the reagent and the sample, so that the memory 1 can be tightly attached to the temperature regulator 2, further, the contact area between the memory 1 and the temperature regulator 2 is increased, and the heat exchange between the temperature regulator 2 and the memory 1 is favorably finished.
Because the manufacturing cost of the soft membrane is lower, compared with the scheme of storing samples by adopting test tubes in the prior art, the scheme has the advantages of low cost and high reaction speed.
In some embodiments, the temperature regulator 2 includes a first temperature regulating portion 201 and a second temperature regulating portion 202, the memory 1 is located between the first temperature regulating portion 201 and the second temperature regulating portion 202, and the memory 1 is supported after a reaction medium is stored in the memory 1 so that the side walls of the memory 1 are in contact with the first temperature regulating portion 201 and the second temperature regulating portion 202, respectively.
In this embodiment, the temperature regulator 2 respectively from both sides to the operation of heating and cooling of memory 1, improved the heat exchange efficiency of temperature regulator 2 with memory 1 greatly, and then improved the reaction efficiency of sample.
A gap 203 for allowing light to pass is formed between the first temperature adjustment portion 201 and the second temperature adjustment portion 202, the memory 1 includes a folded portion 101, and at least a part of the folded portion 101 is located in the gap 203.
Generally, an optical instrument is needed in the detection process of the sample, and after the optical instrument inputs a light ray into the memory 1, the light ray is reflected by the sample and then is collected and analyzed by the corresponding optical instrument, so that the detection of the sample is realized. The gap 203 can be effectively matched with the work of an optical instrument, so that the structure of the sample reaction structure is simplified, and the use cost of the sample reaction structure is reduced.
The storage 1 includes a storage chamber, and a contact area of the storage 1 with the first temperature adjustment portion 201 and the second temperature adjustment portion 202 is not smaller than a side surface area of the storage chamber having a maximum area.
By the shape of the storage chamber being a cuboid, the area of two opposing sides of the cuboid is generally greater than the area of the other sides, e.g. the sides formed by the length and height directions of the storage chamber as shown in the figures are greater in area than the other sides. In practical use, the larger the contact area between the reservoir 1 and the temperature controller 2, the faster the reaction speed, and therefore, the side of the reservoir having a larger area should be in contact with the temperature controller 2.
In some embodiments, the reservoir 1 further comprises a seal 102 forming a storage chamber, the seal 102 being perpendicular to the fold 101.
The width of the sealing edge 102 is not less than twice the thickness of the film from which the reservoir 1 is made.
A temperature adjusting area is formed between the first temperature adjusting part 201 and the second temperature adjusting part 202, and the sealing edge 102 is located outside the temperature adjusting area.
The edge sealing 102 is a process of closing a part of the side edge of the storage cavity by adopting a heat sealing process, generally melts the soft film, then compresses the melted part of the soft film, and forms the edge sealing after the soft film is cooled.
Generally, the wider the seal 102, the better the sealing of the storage chamber, and therefore, the seal 102 should have a certain width, and in view of the cost, the width of the seal 102 is not less than twice the thickness of the flexible film of which the storage container 1 is made, which has better sealing performance and lower cost.
The hem seal 102 is formed using a heat sealing process, and thus, in order to provide better stability to the hem seal 102 during use, the hem seal 102 should not be located in a temperature regulated area to avoid damage to the hem seal 102.
The technical scheme provided by the disclosure can be applied to any sample detection equipment, such as a fluorescent quantitative PCR instrument and other instruments.
In use, the sample is placed in the storage chamber, and the reservoir 1 is provided with an opening communicating with the storage chamber, and the sample can be pushed into the storage chamber by squeezing. For example, a sample pushing mechanism pushes the sample into the storage chamber. Specifically, the storage chamber extends along the opening to form a channel through which the sample enters the storage chamber. The sample pushing mechanism is used for pushing the sample in the channel into the storage cavity. After the sample pushing mechanism pushes the sample into the storage cavity, the sample pushing mechanism is kept at the position so as to play a role in sealing the storage cavity and avoid the sample in the storage cavity from leaking.
The specific structure of the sample pushing mechanism is not limited and can be freely selected. The push mechanism seals the storage chamber optionally, and the storage chamber may be sealed or unsealed by other mechanisms.
The reaction in the present disclosure refers to the process of cycling the sample between high and low temperatures to achieve sample detection.
While the subject matter of the present disclosure and its corresponding details have been described above, it is to be understood that the above description is only illustrative of some embodiments of the subject matter of the present disclosure and that some of the details may be omitted from the detailed description.
In addition, in some of the embodiments disclosed above, there is a possibility that a plurality of embodiments may be combined and implemented, and various combinations are not listed at length. The implementation embodiments can be freely combined according to the requirements when the technical personnel in the field carry out the implementation so as to obtain better application experience.
Other configurations of details or figures may be derived by those skilled in the art in practicing the presently disclosed subject matter, as well as figures, and it will be apparent that such details are within the scope of the presently disclosed subject matter and are covered by the presently disclosed subject matter without departing from the presently disclosed subject matter.
Claims (8)
1. A sample reaction structure, characterized in that: the temperature controller comprises a memory (1) for storing a reaction medium and a temperature controller (2) for repeatedly heating and cooling a sample, wherein the memory (1) is formed by folding a same bendable flexible film, and the memory (1) is supported after the reaction medium is stored in the memory (1) so that the side wall of the memory (1) is in contact with the temperature controller (2).
2. The sample reaction structure according to claim 1, wherein: the temperature regulator (2) comprises a first temperature regulating part (201) and a second temperature regulating part (202), the memory (1) is positioned between the first temperature regulating part (201) and the second temperature regulating part (202), and after a reaction medium is stored in the memory (1), the memory (1) is supported to enable the side wall of the memory (1) to be respectively contacted with the first temperature regulating part (201) and the second temperature regulating part (202).
3. The sample reaction structure according to claim 2, wherein: a gap (203) allowing light to pass is formed between the first temperature adjustment part (201) and the second temperature adjustment part (202), the memory (1) comprises a folding part (101), and at least one part of the folding part (101) is located in the gap (203).
4. The sample reaction structure according to claim 3, wherein: the storage device (1) comprises a storage cavity, and the contact area of the storage device (1) with the first temperature adjusting part (201) and the second temperature adjusting part (202) is not smaller than the side surface area of the storage cavity with the largest area.
5. The sample reaction structure according to claim 4, wherein: the reservoir (1) further comprises a seal (102) forming a storage chamber, the seal (102) being perpendicular to the fold (101).
6. The sample reaction structure according to claim 5, wherein: the width of the sealing edge (102) is not less than twice the thickness of the soft film for manufacturing the memory (1).
7. The sample reaction structure according to claim 5, wherein: a temperature adjusting area is formed between the first temperature adjusting part (201) and the second temperature adjusting part (202), and the sealing edge (102) is positioned outside the temperature adjusting area.
8. The sample reaction structure according to any one of claims 1 to 7, wherein: the temperature regulator (2) is a semiconductor temperature regulating sheet.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120717262.5U CN214654977U (en) | 2021-04-08 | 2021-04-08 | Sample reaction structure |
| PCT/CN2021/134987 WO2022213631A1 (en) | 2021-04-08 | 2021-12-02 | Sample reaction structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120717262.5U CN214654977U (en) | 2021-04-08 | 2021-04-08 | Sample reaction structure |
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| CN214654977U true CN214654977U (en) | 2021-11-09 |
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| CN202120717262.5U Active CN214654977U (en) | 2021-04-08 | 2021-04-08 | Sample reaction structure |
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| WO (1) | WO2022213631A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022213631A1 (en) * | 2021-04-08 | 2022-10-13 | 埃妥生物科技(杭州)有限公司 | Sample reaction structure |
| CN117327571A (en) * | 2023-11-30 | 2024-01-02 | 广州国家实验室 | Carrier and application thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006038643A1 (en) * | 2004-10-06 | 2006-04-13 | Universal Bio Research Co., Ltd. | Reaction container and reaction controller |
| CN102452639A (en) * | 2010-11-01 | 2012-05-16 | 香港理工大学 | Bonding method of plastic microfluidic chip and plastic microfluidic chip |
| WO2013021958A1 (en) * | 2011-08-05 | 2013-02-14 | 株式会社 東芝 | Multiple nucleic acid amplification reaction instrument |
| HK1226023A1 (en) * | 2013-09-18 | 2017-09-22 | 加州理工学院 | System and method for movement and timing control |
| CN214654977U (en) * | 2021-04-08 | 2021-11-09 | 埃妥生物科技(杭州)有限公司 | Sample reaction structure |
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2021
- 2021-04-08 CN CN202120717262.5U patent/CN214654977U/en active Active
- 2021-12-02 WO PCT/CN2021/134987 patent/WO2022213631A1/en active Application Filing
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022213631A1 (en) * | 2021-04-08 | 2022-10-13 | 埃妥生物科技(杭州)有限公司 | Sample reaction structure |
| CN117327571A (en) * | 2023-11-30 | 2024-01-02 | 广州国家实验室 | Carrier and application thereof |
| CN117327571B (en) * | 2023-11-30 | 2024-05-14 | 广州国家实验室 | Carrier and application thereof |
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| Publication number | Publication date |
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| WO2022213631A1 (en) | 2022-10-13 |
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