CN214528976U - Heating device for be used for nucleic acid extraction - Google Patents

Abstract

The utility model relates to a nucleic acid draws technical field, and discloses a heating device for nucleic acid draws, this heating device for nucleic acid draws includes box and rotary mechanism, the heating plate is evenly installed to the box inner chamber, the heat insulating board is installed to the box inner chamber, the motor is installed to box inner chamber bottom, the motor output end rotates and is connected with first bull stick, first runner is installed to first bull stick lateral wall, first bull stick top runs through the heat insulating board and upwards extends and rotate and be connected with the second runner, rotary mechanism includes the third runner, the third runner is located the heat insulating board top and meshes with the second runner mutually, the third runner is kept away from heat insulating board one end fixedly connected with pivot, the carousel is installed to the pivot lateral wall, the utility model discloses compare current device and can make being heated of nucleic acid extract become more even, And the method is sufficient, and the result obtained in the experiment is more accurate.

Description

Heating device for be used for nucleic acid extraction

Technical Field

The utility model belongs to the technical field of nucleic acid extraction, specifically be a heating device for be used for nucleic acid extraction.

Background

The nucleic acid comprises two molecules of DNA and RNA, exists in a state of being combined with protein in cells, and comprises the following main steps of: lysing cells to remove proteins and biomacromolecules such as polysaccharides and lipids combined with nucleic acids, removing other unwanted nucleic acid molecules, precipitating nucleic acids, purifying nucleic acids, removing impurities such as salts and organic agents.

The extracted nucleic acid extracting solution needs to be placed at a certain temperature to ensure the quality, so the nucleic acid extracting solution needs to be heated, the existing heating device for extracting nucleic acid basically uses a heating plate, the nucleic acid extracting solution is difficult to be heated uniformly, the temperature difference exists between the center and the edge of the liquid, the accuracy of the experimental result is directly influenced, and the improvement is needed for the current situation.

SUMMERY OF THE UTILITY MODEL

To the above situation, for overcoming prior art's defect, the utility model provides a heating device for be used for nucleic acid extraction, the effectual current heating device that is used for nucleic acid extraction that has solved is difficult to make the nucleic acid extract be heated evenly, and there is the difference at liquid center and marginal temperature, can directly influence the problem of the accuracy of experimental result.

In order to achieve the above object, the utility model provides a following technical scheme: a heating device for nucleic acid extraction comprises a box body and a rotating mechanism, wherein the rotating mechanism is arranged in an inner cavity of the box body;

the heating plates are uniformly arranged in the inner cavity of the box body, the heat insulation plate is arranged in the inner cavity of the box body, the motor is arranged at the bottom of the inner cavity of the box body, the output end of the motor is rotatably connected with a first rotating rod, a first rotating wheel is arranged on the outer side wall of the first rotating rod, the first rotating wheel is positioned below the heat insulation plate, and the top end of the first rotating rod penetrates through the heat insulation plate to extend upwards and is rotatably connected with a second rotating wheel;

the rotating mechanism comprises a third rotating wheel, the third rotating wheel is positioned above the heat insulation plate and meshed with the second rotating wheel, one end of the third rotating wheel, which is far away from the heat insulation plate, is fixedly connected with a rotating shaft, the inner cavity of the rotating shaft is rotatably connected with a second rotating rod, the second rotating rod penetrates through the heat insulation plate and is connected with the bottom of the inner cavity of the box body, the outer side wall of the second rotating rod is respectively provided with a fourth rotating wheel and a fifth rotating wheel, the fourth rotating wheel is meshed with the first rotating wheel, the fifth rotating wheel is positioned above the rotating shaft, the outer side wall of the rotating shaft is provided with a rotating disc, the top of the rotating disc is uniformly provided with a fixed block, the outer side wall of the fixed block is rotatably connected with a sixth rotating wheel, the sixth rotating wheel is meshed with the fifth rotating wheel, one side, which is far away from the rotating shaft, of the fixed block is provided with a fixed plate, the top of the fixed plate is rotatably connected with a seventh rotating wheel, and the seventh rotating wheel is meshed with the sixth rotating wheel, and the top of the seventh rotating wheel is fixedly connected with a limiting ring.

Preferably, support columns are uniformly installed at the bottom of the inner cavity of the box body, and the tops of the support columns are connected with the heat insulation plate.

Preferably, a bearing is installed at the top of the heat insulation plate, and the third rotating wheel is rotatably connected to an inner ring of the bearing.

Preferably, the turntable is evenly provided with through holes, the inner ring of the limiting ring is provided with a fixed pipe, and the fixed pipe penetrates through the fixed plate and extends downwards into the through holes.

Preferably, the top of the fixing block is rotatably connected with a protection plate, and the protection plate is connected with the top of the fixing pipe when in a closed state.

Preferably, the inner ring of the limiting ring is uniformly provided with limiting blocks, and the limiting blocks are connected with the outer wall of the fixed pipe.

Compared with the prior art, the beneficial effects of the utility model are that: the nucleic acid extracting solution is driven to rotate in the box body through the turntable, and simultaneously, the nucleic acid extracting solution automatically rotates in the fixing tube, so that the nucleic acid extracting solution can be heated more uniformly and fully compared with the conventional device, and the result obtained in an experiment is more accurate;

when realizing that the extraction tube rotates along with the carousel, extraction tube self also rotates in the fixed tube to realize that the nucleic acid extract autogyration is in the extraction tube, make the heating become more even, abundant, the effect is better.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

In the drawings:

fig. 1 is a cross-sectional view of the present invention;

FIG. 2 is a schematic structural view of the present invention;

fig. 3 is a top view of the present invention;

in the figure: 100. a box body; 101. a heating plate; 102. a heat insulation plate; 103. a motor; 104. a first rotating lever; 105. a first runner; 106. a second runner; 107. a support pillar; 108. a bearing; 200. a rotation mechanism; 201. a third rotating wheel; 202. a rotating shaft; 203. a second rotating rod; 204. a fourth rotating wheel; 205. a fifth rotating wheel; 206. a turntable; 207. a fixed block; 208. a sixth rotating wheel; 209. a fixing plate; 210. a seventh rotating wheel; 211. a limiting ring; 212. a fixed tube; 213. a protection plate; 214. and a limiting block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1, a heating apparatus for nucleic acid extraction includes a case 100 and a rotating mechanism 200.

Referring to fig. 1, the rotating mechanism 200 is installed in the chamber of the box 100.

Referring to fig. 1-3, heating sheets 101 are uniformly installed in an inner cavity of a box body 100, a heat insulation plate 102 is installed in the inner cavity of the box body 100, a motor 103 is installed at the bottom of the inner cavity of the box body 100, an output end of the motor 103 is rotatably connected with a first rotating rod 104, a first rotating wheel 105 is installed on the outer side wall of the first rotating rod 104, the first rotating wheel 105 is located below the heat insulation plate 102, and the top end of the first rotating rod 104 penetrates through the heat insulation plate 102 to extend upwards and is rotatably connected with a second rotating wheel 106;

supporting columns 107 are uniformly arranged at the bottom of the inner cavity of the box body 100, the tops of the supporting columns 107 are connected with the heat insulation plate 102, a bearing 108 is arranged at the top of the heat insulation plate 102, and a third rotating wheel 201 is rotatably connected to the inner ring of the bearing 108;

the supporting columns 107 are arranged at the bottom of the inner cavity of the box body 100, so that the heat insulation plate 102 can be supported, the device is more stable in rotation, the bearing 108 is arranged at the top of the heat insulation plate 102, the third rotating wheel 201 is arranged and rotatably connected to the inner ring of the bearing 108, friction between the third rotating wheel 201 and the heat insulation plate 102 can be reduced when the rotating disc 206 rotates, and the device is effectively protected.

Referring to fig. 1-3, the rotating mechanism 200 includes a third rotating wheel 201, the third rotating wheel 201 is located above the heat-insulating plate 102 and engaged with the second rotating wheel 106, one end of the third rotating wheel 201 away from the heat-insulating plate 102 is fixedly connected with a rotating shaft 202, an inner cavity of the rotating shaft 202 is rotatably connected with a second rotating rod 203, the second rotating rod 203 penetrates through the heat-insulating plate 102 and is connected with the bottom of the inner cavity of the box body 100, the outer side wall of the second rotating rod 203 is respectively provided with a fourth rotating wheel 204 and a fifth rotating wheel 205, the fourth rotating wheel 204 is engaged with the first rotating wheel 105, the fifth rotating wheel 205 is located above the rotating shaft 202, the outer side wall of the rotating shaft 202 is provided with a rotating disc 206, the top of the rotating disc 206 is uniformly provided with a fixed block 207, the outer side wall of the fixed block 207 is rotatably connected with a sixth rotating wheel 208, the sixth rotating wheel 208 is engaged with the fifth rotating wheel 205, one side of the fixed block 207 away from the rotating shaft 202 is provided with a fixed plate 209, the top of the fixed plate 209 is rotatably connected with a seventh rotating wheel 210, the seventh rotating wheel 210 is meshed with the sixth rotating wheel 208, and the top of the seventh rotating wheel 210 is fixedly connected with a limiting ring 211;

through holes are uniformly formed in the turntable 206, a fixing tube 212 is mounted on the inner ring of the limiting ring 211, the fixing tube 212 penetrates through the fixing plate 209 and extends downwards into the through holes, the top of the fixing block 207 is rotatably connected with a protection plate 213, the protection plate 213 is connected with the top of the fixing tube 212 when in a closed state, limiting blocks 214 are uniformly mounted on the inner ring of the limiting ring 211, and the limiting blocks 214 are connected with the outer wall of the fixing tube 212;

through installing fixed pipe 212 at the spacing ring 211 inner circle, can assist the extraction tube that is equipped with the nucleic acid extract to lay, through installing stopper 214 at the spacing ring 211 inner circle, can inject the position of fixed pipe 212, make it more stable in the heating process, can not rock, through installing protection shield 213 at fixed block 207 top, can prevent that the nucleic acid extract in the fixed pipe 212 from spilling or having debris to get into the extraction tube and causing the pollution at rotatory in-process.

The working principle is as follows: firstly, an extraction tube filled with nucleic acid extracting solution is placed in a fixed tube 212, a protective plate 213 is rotated to cover the top of the fixed tube 212, so that a heating plate 101 works, a motor 103 is started, the motor 103 drives a first rotating rod 104 to rotate, the first rotating rod 104 drives a first rotating wheel 105 and a second rotating wheel 106 to rotate, the second rotating wheel 106 drives a third rotating wheel 201 to rotate in an inner ring of a bearing 108, the third rotating wheel 201 drives a rotating shaft 202 to rotate, the rotating shaft 202 drives a rotating disc 206 arranged on the outer side wall of the rotating shaft to rotate, the rotating disc 206 drives a fixed block 207 to rotate, the fixed block 207 drives a fixed plate 209 and a fixed tube 212 positioned in a through hole to rotate, the extraction tube rotates along with the rotating disc 206 in an inner cavity of a box body 100, so that the heating of the nucleic acid extracting solution is uniform, the fourth rotating wheel 204 is meshed with the first rotating wheel 105, and the fourth rotating wheel 204 drives a second rotating rod 203 to rotate, and then drive fifth runner 205 and rotate, fifth runner 205 drives sixth runner 208 on the fixed block 207 and rotates, sixth runner 208 drives seventh runner 210 and rotates, seventh runner 210 drives spacing ring 211 and is located the fixed tube 212 of spacing ring 211 inner circle and rotates, thereby when realizing that the extraction tube rotates along with carousel 206, extraction tube self also rotates in fixed tube 212, thereby realize that the nucleic acid extract is at the intraductal autogyration of extraction, make the heating become more even, abundant, the effect is better.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. A heating device for nucleic acid extraction, comprising a case (100) and a rotating mechanism (200), characterized in that: the rotating mechanism (200) is arranged in the inner cavity of the box body (100);

heating sheets (101) are uniformly installed in the inner cavity of the box body (100), a heat insulation plate (102) is installed in the inner cavity of the box body (100), a motor (103) is installed at the bottom of the inner cavity of the box body (100), the output end of the motor (103) is rotatably connected with a first rotating rod (104), a first rotating wheel (105) is installed on the outer side wall of the first rotating rod (104), the first rotating wheel (105) is located below the heat insulation plate (102), and the top end of the first rotating rod (104) penetrates through the heat insulation plate (102) to extend upwards and is rotatably connected with a second rotating wheel (106);

the rotating mechanism (200) comprises a third rotating wheel (201), the third rotating wheel (201) is located above the heat insulation plate (102) and is meshed with a second rotating wheel (106), one end of the heat insulation plate (102) is fixedly connected with a rotating shaft (202) by the third rotating wheel (201), a second rotating rod (203) is connected with the inner cavity of the rotating shaft (202) in a rotating mode, the second rotating rod (203) penetrates through the heat insulation plate (102) and is connected with the bottom of the inner cavity of the box body (100), a fourth rotating wheel (204) and a fifth rotating wheel (205) are installed on the outer side wall of the second rotating rod (203) respectively, the fourth rotating wheel (204) is meshed with a first rotating wheel (105), the fifth rotating wheel (205) is located above the rotating shaft (202), a rotating disc (206) is installed on the outer side wall of the rotating shaft (202), a fixing block (207) is evenly installed on the top of the rotating disc (206), and a sixth rotating wheel (208) is connected on the outer side wall of the fixing block (207), sixth runner (208) and fifth runner (205) mesh mutually, fixed block (207) are kept away from pivot (202) one side and are installed fixed plate (209), fixed plate (209) top is rotated and is connected with seventh runner (210), seventh runner (210) mesh mutually with sixth runner (208), seventh runner (210) top fixedly connected with spacing ring (211).

2. The heating device for nucleic acid extraction according to claim 1, wherein: supporting columns (107) are uniformly installed at the bottom of the inner cavity of the box body (100), and the tops of the supporting columns (107) are connected with the heat insulation plate (102).

3. The heating device for nucleic acid extraction according to claim 1, wherein: and a bearing (108) is installed at the top of the heat insulation plate (102), and the third rotating wheel (201) is rotatably connected to the inner ring of the bearing (108).

4. The heating device for nucleic acid extraction according to claim 1, wherein: through holes are uniformly formed in the turntable (206), a fixed pipe (212) is installed on the inner ring of the limiting ring (211), and the fixed pipe (212) penetrates through the fixed plate (209) and extends downwards into the through holes.

5. A heating apparatus for nucleic acid extraction according to claim 1 or 4, characterized in that: the top of the fixing block (207) is rotatably connected with a protection plate (213), and the protection plate (213) is connected with the top of the fixing pipe (212) when in a closed state.

6. A heating apparatus for nucleic acid extraction according to claim 1 or 4, characterized in that: and the inner ring of the limiting ring (211) is uniformly provided with a limiting block (214), and the limiting block (214) is connected with the outer wall of the fixed pipe (212).

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