CN213924869U - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- CN213924869U CN213924869U CN202022922737.2U CN202022922737U CN213924869U CN 213924869 U CN213924869 U CN 213924869U CN 202022922737 U CN202022922737 U CN 202022922737U CN 213924869 U CN213924869 U CN 213924869U
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- heating
- incubator
- constant temperature
- glass
- observation device
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- 238000004113 cell culture Methods 0.000 title claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 100
- 238000010438 heat treatment Methods 0.000 claims abstract description 79
- 239000011521 glass Substances 0.000 claims abstract description 45
- 239000008236 heating water Substances 0.000 claims abstract description 11
- 238000011534 incubation Methods 0.000 claims description 15
- 238000005485 electric heating Methods 0.000 claims description 12
- 239000012930 cell culture fluid Substances 0.000 claims description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 6
- 235000017491 Bambusa tulda Nutrition 0.000 claims 6
- 241001330002 Bambuseae Species 0.000 claims 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 6
- 239000011425 bamboo Substances 0.000 claims 6
- 229920000297 Rayon Polymers 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- 239000006059 cover glass Substances 0.000 abstract description 5
- 230000037237 body shape Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 14
- 238000010367 cloning Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device, the inboard of water bath constant temperature incubator be provided with flexible culture box body shape assorted recess, be hollow column structure in the water bath constant temperature incubator, pack constant temperature heating water in the water bath constant temperature incubator, constant temperature heating water heats through the heating of circulation heating device, the upper end edge and the cultivation box go-between fixed connection of flexible culture box body, the lower extreme of flexible culture box body and the marginal fixed connection of glass cultivation box bottom plate, cell culture liquid tissue is located the flexible culture box body, fixed connection can be dismantled with the upper end of cultivateing the box go-between to glass cultivation box apron. The utility model discloses a set up the cultivation box of integral type, at the in-process of cultivateing and observing all need not take out the culture cell, and cultivate the box and act as cover glass and slide glass automatically, can not lead to the cell liveness to descend because of the difference in temperature during the observation yet to improved the experimental accuracy, makeed the cultivation observation result accurate.
Description
Technical Field
The utility model relates to a biological assay equipment especially relates to a cell culture device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched. Among the prior art, cell culture cultivates in the culture dish mostly, heating constant temperature needs go on in the incubator, and after the cultivation finishes, observe and need take out, arrange in on microscopical cover glass and the slide again, the device can contact with the air, also pollute easily, complex operation, it is not accurate to lead to detecting the observation result, and the cell difference in temperature that cover glass and slide and cultivateed is great, the activity of the cell has also been influenced to a certain extent, thereby lead to the verification degree of accuracy decline of culture result, therefore, there is the improvement space.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to solving the above problems and to providing a cell culture apparatus.
The utility model discloses a following technical scheme realizes above-mentioned purpose:
the utility model relates to a cell culture device, which comprises a water bath constant temperature incubator, constant temperature heating water, an incubator connecting ring, a flexible incubator box body, a glass incubator bottom plate, a glass incubator cover plate, cell culture fluid tissues and a circulating heating device, wherein the inner side of the water bath constant temperature incubator is provided with a groove matched with the shape of the flexible incubator box body, the water bath constant temperature incubator is internally provided with a hollow structure, the water bath constant temperature incubator is filled with the constant temperature heating water, the constant temperature heating water is heated by the circulating heating device, the upper end edge of the flexible incubator box body is fixedly connected with the incubator connecting ring, the lower end of the flexible incubator box body is fixedly connected with the edge of the glass incubator bottom plate, the cell culture fluid tissues are positioned in the flexible incubator box body, the glass incubator cover plate is detachably and fixedly connected with the upper end of the incubator connecting ring, the flexible culture box body and the glass culture box bottom plate are partially positioned in the groove of the water bath constant temperature culture box and can be taken out.
Further, the circulation heating device comprises a heating circulation water pump, a circulation water heating coil, a coil heating electric heating wire, a circulation water storage tank and a circulation water return pipe, the water inlet of the water bath constant temperature incubator is connected with the water outlet of the heating circulation water pump, the water outlet of the water bath constant temperature incubator is connected with the water inlet of the circulation water storage tank through the circulation water return pipe, the water outlet of the circulation water storage tank is connected with the water inlet of the circulation water heating coil, the water outlet of the circulation water heating coil is connected with the water inlet of the heating circulation water pump, and the coil heating electric heating wire is wound on the surface of the circulation water heating coil.
Furthermore, the circulation heating device comprises a heating temperature sensor, the signal output end of the heating temperature sensor is connected with the signal input end of a heating control system, and the control output end of the heating control system is also connected with the heating circulation water pump and the coil heating electric heating wire.
As an improvement, the circulating water heating coil and the coil heating electric heating wire are positioned in the heating coil heat preservation shell.
Preferably, the culture box connecting ring and the glass culture box cover plate are detachably and fixedly connected through glue.
As an improvement, the glass cultivation box is further provided with an observation device, the observation device is composed of an observation device base, an observation device light source, an observation device support barrel and an electron observation microscope, the observation device support barrel is of a cylindrical structure, the observation device base is fixedly arranged at the lower end of the observation device support barrel, the middle part of the observation device base is positioned at the lower end in the observation device support barrel and is provided with the observation device light source, the electron observation microscope is positioned above the observation device support barrel, and the glass cultivation box bottom plate and the glass cultivation box cover plate are positioned at the upper end of the observation device support barrel and are positioned below the electron observation microscope.
Preferably, the upper section in the observation device support barrel is provided with a uniform-light Fresnel lens.
As an improvement, a plurality of grooves are arranged on the water bath constant temperature incubator, and each groove can be internally provided with a culture box consisting of a culture box connecting ring, a flexible culture box body, a glass culture box bottom plate and a glass culture box cover plate.
The beneficial effects of the utility model reside in that:
the utility model relates to a cell culture device, compared with the prior art, the utility model discloses a set up the cultivation box of integral type, at the in-process of cultivateing and observing all need not take out the cultured cell, and cultivate the box and act as cover glass and slide glass automatically, can not lead to the cell liveness to descend because of the difference in temperature during the observation yet to improved the experimental accuracy, makeed the cultivation observation result accurate, convenient to use has the value of popularization and application.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic structural view of the observation device of the present invention;
FIG. 3 is a schematic top view of the culture cassette;
FIG. 4 is a schematic view of a structure of a plurality of cassettes.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings:
as shown in fig. 1-4: the utility model relates to a cell culture device, which comprises a water bath constant temperature incubator 1, constant temperature heating water 2, an incubation box connecting ring 3, a flexible incubation box body 4, a glass incubation box bottom plate 5, a glass incubation box cover plate 6, cell culture fluid tissues 7 and a circulating heating device, wherein the inner side of the water bath constant temperature incubator 1 is provided with a groove matched with the shape of the flexible incubation box body 4, the water bath constant temperature incubator 1 is internally provided with a hollow structure, the water bath constant temperature incubator 1 is filled with the constant temperature heating water 2, the constant temperature heating water 2 is heated by the circulating heating device, the upper end edge of the flexible incubation box body 4 is fixedly connected with the incubation box connecting ring 3, the lower end of the flexible incubation box body 4 is fixedly connected with the edge of the glass incubation box bottom plate 5, and the cell culture fluid tissues 7 are positioned in the flexible incubation box body 4, the glass culture box cover plate 6 is detachably and fixedly connected with the upper end of the culture box connecting ring 3, and the flexible culture box body 4 and the glass culture box bottom plate 5 are partially positioned in the groove of the water bath constant temperature culture box 1 and can be taken out.
Further, the circulation heating device comprises a heating circulation water pump 8, a circulation water heating coil 9, a coil heating electric heating wire 10, a circulation water storage tank 12 and a circulation water return pipe 13, the water inlet of the water bath constant temperature incubator 1 is connected with the water outlet of the heating circulation water pump 8, the water outlet of the water bath constant temperature incubator 1 is connected with the water inlet of the circulation water storage tank 12 through the circulation water return pipe 13, the water outlet of the circulation water storage tank 12 is connected with the water inlet of the circulation water heating coil 9, the water outlet of the circulation water heating coil 9 is connected with the water inlet of the heating circulation water pump 8, and the coil heating electric heating wire 10 is wound on the surface of the circulation water heating coil 9.
Further, the circulation heating device comprises a heating temperature sensor 14, a signal output end of the heating temperature sensor 14 is connected with a signal input end of a heating control system 15, and a control output end of the heating control system 15 is further connected with the heating circulation water pump 8 and the coil heating electric heating wire 10.
As an improvement, the circulating water heating coil 9 and the coil heating electric heating wire 10 are positioned in a heating coil heat preservation shell 11.
Preferably, the culture box connecting ring 3 and the glass culture box cover plate 6 are detachably and fixedly connected through glue.
As an improvement, an observation device is further provided, the observation device is composed of an observation device base 16, an observation device light source 17, an observation device support barrel 18 and an electron observation microscope 20, the observation device support barrel 18 is of a cylindrical structure, the observation device base 16 is fixedly arranged at the lower end of the observation device support barrel 18, the observation device light source 17 is arranged at the lower end of the middle part of the observation device base 16, which is positioned in the observation device support barrel 18, the electron observation microscope 20 is positioned above the observation device support barrel 18, and the glass culture box bottom plate 5 and the glass culture box cover plate 6 are positioned at the upper end of the observation device support barrel 18 and below the electron observation microscope 20.
Preferably, the upper section in the observation device bracket barrel 18 is provided with a uniform-light Fresnel lens 19.
As an improvement, a plurality of grooves are arranged on the water bath constant temperature incubator 1, and each groove can be internally provided with an incubator composed of an incubator connecting ring 3, a flexible incubator box body 4, a glass incubator bottom plate 5 and a glass incubator cover plate 6.
The utility model relates to a cell culture method, which comprises the following steps:
firstly, when cells need to be cultured, placing cell culture solution tissues 7 into a flexible culture box body 4, and bonding and sealing a glass culture box cover plate 6 and a culture box connecting ring 3;
placing a culture box consisting of a culture box connecting ring 3, a flexible culture box body 4, a glass culture box bottom plate 5 and a glass culture box cover plate 6 into a groove of the water bath constant temperature culture box 1;
pumping water in a circulating water storage tank 12 into a water bath constant temperature incubator 1 through a heating circulating water pump 8, heating the incubator at constant temperature, heating a circulating water heating coil 9 through a coil heating electric heating wire 10 in the pumping process, monitoring the temperature in the water bath constant temperature incubator 1 through a heating temperature sensor 14, and controlling the heating temperature of the coil heating electric heating wire 10 through monitoring temperature data of the heating temperature sensor 14 by a heating control system 15; the water in the water bath constant temperature incubator 1 returns to the circulating water storage tank 12 through the circulating water return pipe 13 for storage;
fourthly, directly taking out the culture box and entering an observation device for observation when the cell culture is finished;
due to the fact that the box body 4 of the flexible culture box of the culture box is flexible, the glass culture box bottom plate 5 and the glass culture box cover plate 6 are made of glass, the glass culture box bottom plate 5 is placed on the observation device support cylinder 18, the glass culture box bottom plate 5 is overlapped with the glass culture box cover plate 6, the effect of the cover glass and the glass slide is equivalent, and the redundant cell culture solution tissues 7 are located in the flexible culture box body 4;
sixthly, the observation device is powered on to emit light, and the light source is subjected to light equalization by the uniform light Fresnel lens 19, and at this time, observation is performed by the electron observation microscope 20.
The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A cell culture apparatus, characterized by: comprises a water bath constant temperature incubator (1), constant temperature heating water (2), an incubator connecting ring (3), a flexible incubator box body (4), a glass incubator bottom plate (5), a glass incubator cover plate (6), cell culture fluid tissues (7) and a circulating heating device, wherein a groove matched with the shape of the flexible incubator box body (4) is arranged on the inner side of the water bath constant temperature incubator (1), the water bath constant temperature incubator (1) is internally provided with a hollow structure, the constant temperature heating water (2) is filled in the water bath constant temperature incubator (1), the constant temperature heating water (2) is heated by the circulating heating device, the upper end edge of the flexible incubator box body (4) is fixedly connected with the incubator connecting ring (3), the lower end of the flexible incubator box body (4) is fixedly connected with the edge of the glass incubator bottom plate (5), cell culture solution tissue (7) are located in the flexible culture box body (4), the glass culture box cover plate (6) and the upper end of the culture box connecting ring (3) can be detachably and fixedly connected, and the parts of the flexible culture box body (4) and the glass culture box bottom plate (5) are located in the groove of the water bath constant-temperature culture box (1) and can be taken out.
2. The cell culture apparatus of claim 1, wherein: circulation heating device comprises heating circulating water pump (8), circulating water heating coil (9), coil heating wire (10), circulating water storage tank (12), circulating water wet return (13), the water inlet of water bath constant temperature incubator (1) with the delivery port of heating circulating water pump (8) is connected, the delivery port of water bath constant temperature incubator (1) pass through circulating water wet return (13) with the water inlet of circulating water storage tank (12) is connected, the outlet of circulating water storage tank (12) with the water inlet of circulating water heating coil (9) is connected, the outlet of circulating water heating coil (9) with the water inlet of heating circulating water pump (8) is connected, coil heating wire (10) coiling is in the surface of circulating water heating coil (9).
3. The cell culture apparatus of claim 2, wherein: the circulation heating device comprises a heating temperature sensor (14), the signal output end of the heating temperature sensor (14) is connected with the signal input end of a heating control system (15), and the control output end of the heating control system (15) is also connected with the heating circulation water pump (8) and the coil heating electric heating wire (10).
4. The cell culture apparatus of claim 2, wherein: the circulating water heating coil (9) and the coil heating electric heating wire (10) are positioned in the heating coil heat-insulating shell (11).
5. The cell culture apparatus of claim 1, wherein: the culture box connecting ring (3) and the glass culture box cover plate (6) are detachably and fixedly connected through viscose.
6. The cell culture apparatus of claim 1, wherein: still be provided with observation device, observation device comprises observation device base (16), observation device light source (17), observation device support section of thick bamboo (18) and electron observation microscope (20), observation device support section of thick bamboo (18) are cylindric structure, observation device base (16) fixed set up in the lower extreme of observation device support section of thick bamboo (18), the middle part of observation device base (16) is located lower extreme setting in observation device support section of thick bamboo (18) observation device light source (17), electron observation microscope (20) are located the top of observation device support section of thick bamboo (18), glass culture box bottom plate (5) with glass culture box apron (6) are located the upper end of observation device support section of thick bamboo (18) is and are located the below of electron observation microscope (20).
7. The cell culture apparatus of claim 6, wherein: the upper section in the observation device bracket barrel (18) is provided with a uniform-light Fresnel lens (19).
8. The cell culture apparatus of claim 1, wherein: the water bath constant temperature incubator (1) is provided with a plurality of grooves, and each groove can be internally provided with an incubation box consisting of an incubation box connecting ring (3), a flexible incubation box body (4), a glass incubation box bottom plate (5) and a glass incubation box cover plate (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022922737.2U CN213924869U (en) | 2020-12-08 | 2020-12-08 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022922737.2U CN213924869U (en) | 2020-12-08 | 2020-12-08 | Cell culture device |
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| Publication Number | Publication Date |
|---|---|
| CN213924869U true CN213924869U (en) | 2021-08-10 |
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| CN202022922737.2U Active CN213924869U (en) | 2020-12-08 | 2020-12-08 | Cell culture device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112322495A (en) * | 2020-12-08 | 2021-02-05 | 泉州伟业生物医学科技有限公司 | Cell culture device and cell culture method |
| CN113789258A (en) * | 2021-09-06 | 2021-12-14 | 上海塔望智能科技有限公司 | Cell culture device for observation |
-
2020
- 2020-12-08 CN CN202022922737.2U patent/CN213924869U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112322495A (en) * | 2020-12-08 | 2021-02-05 | 泉州伟业生物医学科技有限公司 | Cell culture device and cell culture method |
| CN112322495B (en) * | 2020-12-08 | 2025-03-18 | 泉州伟业生物医学科技有限公司 | A cell culture device and a cell culture method |
| CN113789258A (en) * | 2021-09-06 | 2021-12-14 | 上海塔望智能科技有限公司 | Cell culture device for observation |
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